Amino acid liquid fermented feed as well as preparation method and application thereof

Abstract

The invention relates to the technical field of fermented feed, and particularly discloses amino acid liquid fermented feed as well as a preparation method and application thereof, and the technical key points are as follows: the amino acid liquid fermented feed is prepared by mixing and fermenting dried amino acid liquid and rice bran according to a ratio of 1: 1; the preparation method comprises the following steps: mixing the dried amino acid liquid and the rice bran according to a ratio of 1: 1, controlling the water content of the mixture to be 37.5%, controlling the inoculum size of microorganisms to be 12.2%, controlling the fermentation temperature to be 30 DEG C, and fermenting for 15 days to obtain the amino acid liquid fermented feed. The amino acid liquid fermented feed disclosed by the invention is applied to preparation of a feed for fattening cattle. The amino acid liquid fermented feed can replace soybean meal, has no influence on the growth performance, nutrient apparent digestibility, blood biochemical indexes and rumen flora diversity of the fattening cattle, and provides scientific reference for application of the amino acid liquid waste fermented feed in feeding of the fattening cattle.

Description

Technical Field

[0001] The present invention relates to the technical field of fermented feed, and in particular to an amino acid liquid fermented feed and a preparation method and application thereof. Background Art

[0002] The implementation of soybean meal reduction and substitution has significant implications for reducing costs, enhancing livestock industry competitiveness, and protecting the environment. The "Three-Year Action Plan for Reducing and Substituting Soybean Meal for Feed" explicitly states that unconventional protein raw materials should be efficiently and safely utilized as feed using processes such as bio-fermentation, high-temperature processing, and enzymatic hydrolysis, along with technologies such as enzyme enhancement and nutrient metabolism regulation. This will expand the comprehensive supply of alternative protein feed resources.

[0003] Amino acid liquid is a by-product of the production of acid-hydrolyzed amino acids. Due to the limitations of the processing and extraction process, the amino acid liquid contains rich free amino acids and NH4Cl. In the early stage, the inventors of this application used amino acid liquid and rice bran meal for mixed fermentation to produce amino acid liquid fermented feed with a crude protein content of 48%, a free amino acid content of 22%, and an NH4Cl content of 4-6%. The microbial fermentation technology effectively increased the true protein content and improved the palatability, but its high NH4Cl content still limits the use of this raw material. Furthermore, ruminants, due to their unique rumen function, can convert non-protein nitrogen into bacterial protein, provide protein resources for the body, and make the utilization of non-protein nitrogen resources possible. Therefore, the use of non-protein nitrogen resources in ruminants can replace part of the soybean meal raw materials, thereby reducing the cost of feed.

[0004] To this end, the inventors of the present application provide an amino acid liquid fermented feed and a preparation method and application thereof to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to solve the technical problems raised in the above background technology and provide an amino acid liquid fermented feed and its preparation method and application.

[0006] The above-mentioned purpose of the present invention is achieved like this:

[0007] On one hand, the solution of the present invention provides an amino acid liquid fermented feed, which is prepared by mixing and fermenting dried amino acid liquid and rice bran in a ratio of 1:1.

[0008] Another aspect of the present invention provides a method for preparing an amino acid liquid fermented feed, the method comprising: mixing dried amino acid liquid and rice bran in a ratio of 1:1, wherein the water content of the mixture is controlled at 37.5%, the inoculation amount of the microorganism is 12.2%, the fermentation temperature is 30°C, and the fermentation is carried out for 15 days to obtain the amino acid liquid fermented feed.

[0009] Another aspect of the present invention further provides the use of the above-mentioned amino acid liquid fermented feed or the preparation method of the amino acid liquid fermented feed in preparing feed for fattening cattle.

[0010] Furthermore, the amino acid liquid fermented feed is used to replace soybean meal raw material.

[0011] Furthermore, the amino acid liquid fermented feed replaces soybean meal in an amount of 10%-30%.

[0012] Furthermore, the amino acid liquid fermented feed replaces soybean meal by 20%.

[0013] The difficulty and significance of the technical problem solved by the present invention are:

[0014] With the growing development of the animal husbandry industry, the amount of feed consumed annually by the animal husbandry industry has increased year by year, and the consumption of protein feed (soybean meal) is even greater. According to the Three-Year Action Plan for Reducing and Substituting Soybean Meal for Feed, the technology of low-protein amino acid balanced diet is promoted. By supplementing essential amino acids (such as L-lysine and L-tryptophan), the amount of soybean meal is reduced. The goal of achieving a "soybean-free diet" is of great significance. After early research and exploration, the inventors of this application designed an amino acid liquid fermented feed to replace soybean meal in different proportions, and confirmed that it has no negative impact on the growth performance, apparent digestibility of nutrients, and rumen environment of fattening cattle, and can improve growth performance and feed utilization, providing scientific reference significance for the application of amino acid waste liquid fermented feed in the feeding of fattening cattle.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. The amino acid liquid fermented feed of the present invention can replace soybean meal and has no effect on the growth performance, apparent nutrient digestibility, blood biochemical indicators and rumen flora diversity of fattening cattle, providing a scientific reference for the application of amino acid waste liquid fermented feed in the feeding of fattening cattle;

[0017] 2. The amino acid waste liquid fermented feed of the present invention has no negative effects in the actual feeding process of fattening cattle, and can improve their growth performance and save costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is the rumen bacteria dilution curve in the embodiment of the present invention;

[0019] Figure 2 is a Venn diagram in an embodiment of the present invention;

[0020] Figure 3 It is the PCoA based on weighted Unifrac distance in the embodiment of the present invention. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0022] The implementation of the present invention is described in detail below with reference to specific embodiments.

[0023] Reference Figure 1-Figure 3 As shown, the following are preferred embodiments provided by the present invention.

[0024] Example: The embodiment of the present invention provides an amino acid liquid fermented feed and its preparation method and application, which aims to replace soybean meal with the amino acid liquid fermented feed of the present invention without negative impact on fattening cattle, and improve their growth performance and feed utilization.

[0025] In an embodiment of the present invention, the preparation method of the amino acid liquid fermented feed is: drying the amino acid liquid and rice bran in a ratio of 1:1, wherein the water content of the mixture is controlled at 37.5%, the inoculation amount of the microorganism is 12.2%, the fermentation temperature is 30°C, and the fermentation is carried out for 15 days to obtain the amino acid liquid fermented feed.

[0026] The following is a specific test of the embodiment of the present invention:

[0027] The purpose of this experiment is to verify the effects of replacing soybean meal with different proportions of amino acid liquid fermented feed on the growth performance, apparent nutrient digestibility, blood biochemical indicators and rumen flora diversity of fattening cattle. The details are as follows:

[0028] 1. Materials and Methods

[0029] 1.1 Test Materials

[0030] Amino acid liquid was purchased from Wuhan Duxinglong Agricultural Technology Co., Ltd., and rice bran was purchased from Wuhan Liangxinxin Planting Professional Cooperative. The fermentation conditions for the fermented feed were as follows: a 1:1 mixture of dried amino acid liquid and rice bran was used, with a moisture content of 37.5%, an inoculum size of 12.2%, a fermentation temperature of 30°C, and fermentation for 15 days. Animal-derived information for the fermented feed is shown in Table 1. The nutritional composition of the dried amino acid liquid is shown in Table 2.

[0031] Table 1 Detection of animal origin of amino acid liquid fermented feed

[0032]

[0033] Table 2 Amino acid composition of drying amino acid waste liquid (%)

[0034]

[0035]

[0036] 1.2 Experimental Design

[0037] This experiment was conducted at the Jingniu Agricultural and Animal Husbandry Cooperative in Jingshan, Hubei Province from January to March 2024. Forty Simmental fattening cattle in good condition, similar in weight and age were randomly divided into four groups, with 10 cattle in each group (10 replicates per group, 1 cattle per replicate). The proportion of fermented feed replacing soybean meal in the four groups' diets was CON (0), T10 (10% soybean meal replacement group), T20 (20% soybean meal replacement group), and T30 (30% soybean meal replacement group), respectively. The feed was fully mixed using a feed mixing machine (TMR) and fed, with ample water provided. The experimental period was 70 days (10 days for pre-feeding and 60 days for main feeding). The diets were formulated in accordance with the "Standards for Beef Cattle Feeding" (NY / T 815-2004). Details of the feed formula and nutrient content of each group are shown in Table 3.

[0038] Table 3 Feed composition and nutritional level (air-dried basis %)

[0039]

[0040]

[0041] in:

[0042] 1) The premix provided the following perkilogram of diets per kilogram of feed: Fe mg, Cu mg, Zn mg, Mn mg, I mg, Co mg, Se mg, VA IU, VD IU, VE IU.

[0043] 2) Net energy for maintenance and net energy for weight gain were calculated with reference to the Table of Feed Composition and Nutritional Value in China (33rd edition 2022), while the others were measured values.

[0044] 1.3 Feeding and Management

[0045] The experiment was conducted at Hubei Liangyou Jinniu Animal Husbandry Technology Co., Ltd. The pens were disinfected before the experiment began. Four groups of experimental cattle were fed a total mixed feed (TMR) in separate pens at 9:00 AM and 4:00 PM daily. Water was changed frequently to ensure clean water for the fattening cattle.

[0046] 1.4. Measurement indicators and methods

[0047] 1.4.1 Growth performance

[0048] At the beginning and end of the experiment, the experimental cattle were weighed on an empty stomach, and the initial and final weights were recorded. Feed intake was also recorded. Based on this, average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F / G) were calculated. The specific calculation formula is as follows:

[0049] Average daily feed intake = (dry matter mass fed - dry matter mass remaining) / number of experimental days;

[0050] Average daily weight gain = (final weight - initial weight) / number of test days;

[0051] Feed-to-weight ratio = average daily feed intake / average daily weight gain.

[0052] 1.4.2 Nutrient Apparent Digestibility

[0053] On the last three days of the experiment, 100g of fresh feces was collected from each cow each time, 20mL of prepared 10% concentrated sulfuric acid was added, and the fecal samples were mixed evenly to fix nitrogen. After the experiment, the fecal samples taken from each cow for three days were mixed evenly, and 150g was randomly selected and stored in a refrigerator at -20℃ for testing. The diet for the last three days was collected by the quartering method. After the experiment, the diet collected for three days was mixed evenly and stored in a refrigerator at -20℃ for testing. The apparent digestibility of nutrients in the feed was determined using hydrochloric acid insoluble ash (AIA) as an indicator. The determination of DM, CP, EE, Ash, NDF, ADF, and OM was carried out in accordance with the "Feed Analysis and Feed Quality Testing Technology" (Zhang Liying, 2021). The digestion test was determined using the endogenous indicator acid insoluble ash method, and the determination method was in accordance with GB / T23742-2009. The calculation formula for the apparent digestibility of a nutrient is as follows:

[0054] Apparent digestibility of a nutrient in the diet (%) = (amount of the nutrient ingested - amount of the nutrient in feces) / amount of the nutrient ingested × 100%

[0055] 1.4.3 Serum biochemistry

[0056] On the 30th day when the experiment officially started, 5 cows were randomly selected from each group for tail root blood collection at 9:00. 20 ml of blood was collected from each selected cow and placed in two 10-ml blood collection tubes respectively. After standing, the supernatant was aspirated by centrifugation and sub-packed into 1.5-ml EP tubes, and stored at -20 °C for further measurement. Total protein (TP), globulin (GLB), albumin (ALB), ammonia (AN), urea nitrogen (BUN), blood glucose (GLU), aspartate aminotransferase (AST), alanine aminotransferase (ALTT), alkaline phosphatase (ALPP), total cholesterol (TC), triglyceride (TG), insulin (INS), and insulin-like growth factor (IGF-1) in the serum were measured.

[0057] 1.5. Data analysis

[0058] The data was used to establish a database in Excel 2016, and analyzed by one-way analysis of variance (One-way ANOVA) and multiple comparisons by LSD method in SPSS 27.0 software. P < 0.05 indicates significant difference, P < 0.01 indicates extremely significant difference, and 0.05 < P < 0.10 indicates a trend. The results were expressed as mean and standard error.

[0059] 2. Analysis of experimental results

[0060] 2.1. Effects of fermented feed replacing different proportions of soybean meal on the growth performance of fattening cattle

[0061] As can be seen from Table 4, there were no significant differences in the final weight, daily weight gain, and feed-to-weight ratio of fattening cattle when fermented feed replaced soybean meal (P > 0.05). However, compared with the CON group, the final weight and daily weight gain of the T10 and T20 replacement groups increased, and the feed-to-meat ratio of the three replacement groups decreased. Among them, the cost per kilogram of weight gain in the T20 group was the lowest, at 12.09 yuan, which was 0.89 yuan lower than that of the soybean meal group.

[0062] Table Effects of fermented feed replacing different proportions of soybean meal on the growth performance of fattening cattle

[0063]

[0064] 2.2. Effects of fermented feed replacing different proportions of soybean meal on the apparent digestibility of nutrients in fattening cattle

[0065] As can be seen from Table 5 below, there were no significant differences in the apparent digestibility of CP, EE, NDF, and ADF in fattening cattle when different proportions of fermented feed were used to replace soybean meal (P > 0.05). However, compared with the CON group, there was an increasing trend in the apparent digestibility of NDF and ADF in the fermented feed replacement groups.

[0066] Table 5 Effects of fermented feed replacing different proportions of soybean meal on the apparent digestibility of nutrients in fattening cattle

[0067]

[0068] 2.3 Effects of fermented feed replacing soybean meal at different proportions on serum biochemical parameters of fattening cattle

[0069] As shown in Table 6, with the increase of fermented feed replacement amount, the AST content gradually increased. The AST in the T30 group was extremely significantly lower than that in the CON group and the T10 group (P<0.01), and significantly lower than that in the T20 group (P<0.05). The triglyceride concentration in the T10 group was extremely significantly higher than that in the CON group, the T20 group, and the T30 group (P<0.01). There were no significant differences in TP, ALB, GLB, ALT, AST / ALT, ALP, UN, TC, GLU, INS, and IGF-1 among the treatment groups (P>0.05).

[0070] Table 6 Effects of fermented feed replacing soybean meal in different proportions on serum biochemical indices of fattening cattle

[0071]

[0072]

[0073] 2.4 Effects of fermented feed replacing soybean meal at different ratios on the richness and diversity of rumen microbiota in fattening cattle

[0074] Depend on Figure 1 As shown in the figure, with the continuous increase in sequencing depth, the dilution curves of the four groups of rumen bacteria tended to be stable, indicating that the sample size can truly reflect the structure of the experimental rumen bacterial fluid.

[0075] Depend on Figure 2 As shown in the figure, a total of 29,678 ASVs were obtained in the four experimental groups. The numbers of ASVs in the CON group, T10 group, T20 group, and T30 group were 11,445, 9,195, 8,723, and 7,594, respectively, showing the order of CON group > T10 group > T20 group > T30 group; the total number of ASVs in the CON group, T10 group, T20 group, and T30 group was 1,158, accounting for 10.12%, 12.59%, 13.28%, and 15.25% of the number of ASVs in each group, respectively.

[0076] As shown in Table 7, the results of the α-diversity analysis of microorganisms showed that the Coverage index of different treatment groups exceeded 99%; there were no significant differences in the Chao1 index and the Observed species index, Shannon index, SimPson index, Faith's PD index, and Pielou's index (P>0.05); compared with the CON group, the number of observed species tended to decrease (0.05<P<0.1).

[0077] Table 7 Effects of fermented feed replacing soybean meal at different ratios on the alpha diversity of rumen microbiota in fattening cattle

[0078]

[0079] Beta diversity research is a comparative study of biodiversity in different habitats or microbial populations to explore the consistency or difference in the population composition of samples in different groups. The distance between samples in the principal coordinate analysis (PCoA) diagram represents the degree of similarity between the two, and the closer the distance, the greater the similarity. Figure 3 As shown in the PCOA diagram based on the weighted-Unifrac distance, in low-dimensional space, the closer the distance between two sample points, the higher their similarity. The sample points of the CON group and the T10, T20, and T30 groups clustered closely (P>0.05), indicating that there were no significant differences in the microbial community structures of the CON, T10, T20, and T30 groups.

[0080] Effects of replacing soybean meal with fermented feed in different proportions on the performance of fattening cattle: Research has shown that microbial fermentation can improve the quality and palatability of animal roughage and improve animal production performance. Fermented miscellaneous meal has been used in different proportions to replace soybean meal in feeding fattening cattle. Fermented miscellaneous meal has no negative impact on growth performance and apparent nutrient digestibility in fattening cattle, and has improved production performance and saved costs. Furthermore, research has found that replacing soybean meal with fermented feed did not significantly affect animal growth performance, but both final weight and average daily gain increased, and the feed-to-weight ratio decreased. Consistent with these results, the experimental results of the above examples of this application showed that replacing soybean meal with fermented amino acid liquid feed did not significantly affect the final weight, average daily gain, and feed-to-weight ratio of the experimental groups, but all showed a trend of improvement. Final weight increased by 1.68%, 1.21%, and 0.34%, average daily gain increased by 10.08%, 8.19%, and 2.73%, and feed-to-weight ratio decreased by 5.79%, 11.55%, and 5.50%, respectively. The above experiments show that replacing soybean meal with amino acid liquid fermented feed has no negative impact on the growth performance and apparent digestibility of nutrients of fattening cattle and can improve production performance to a certain extent.

[0081] Moreover, in the above-mentioned experiment of the present application, soybean meal was calculated at 3600 yuan / ton and fermented feed was calculated at 2000 yuan / ton. The production cost of 1kg of beef using T10, T20, and T30 replacement groups was 0.85 yuan, 0.89 yuan, and 0.37 yuan lower than that of the control group. Based on the fattening weight gain of 450kg of feeder cattle, the cost per head of cattle can be reduced by 382.5 yuan, 399.4 yuan, and 174.0 yuan, respectively. This shows that using the amino acid liquid fermented feed obtained by the present invention to replace soybean meal can effectively reduce the amount of soybean meal used and save costs.

[0082] Regarding the effect of fermented feed replacing soybean meal in different proportions on the apparent digestibility of nutrients in fattening cattle: The apparent digestibility of nutrients is an indicator of the digestibility of feed. This study showed that the use of amino acid liquid fermented feed to replace soybean meal in the fattening cattle diet did not significantly affect the digestibility of dietary crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF). However, compared with the CON group, both NDF and ADF showed an upward trend, with NDF increasing by 10.26%, 14.37%, and 11.71%, respectively, and ADF increasing by 7.90%, 15.58%, and 14.73%, respectively. Xu Zixuan et al. (2021) found that adding 7% microbial fermented feed to dairy cows during in vitro fermentation had no significant effect on NDF and ADF, but it could increase NDF and ADF by 3.76% and 1.98%, respectively, compared with the non-added group. Existing research has found that adding a complex enzyme preparation to Huyang sheep does not significantly affect the apparent digestibility of various nutrients, but can increase NDF and ADF by 12.82% and 7.22%, respectively. This is consistent with the research results of the present invention, suggesting that the mixed fermentation of amino acid solution and rice bran reduces the crude fiber content in the feed, while beneficial bacteria increase the crude protein level in the feed, improving the animal's palatability and enhancing the overall nutritional value of the feed, thereby providing more energy and nutrients for animal growth.

[0083] Regarding the effects of fermented feed replacing soybean meal at varying proportions on serum biochemical indices in fattening cattle: Blood circulation transports nutrients from the digestive system to the body's cells, tissues, and organs. Serum biochemical indices are often used to assess the physiological state of animals. Under normal conditions, NPN entering the rumen is broken down into ammonia. Most of the ammonia is utilized by rumen microorganisms, while a small amount enters the bloodstream through the rumen wall. Free ammonia that enters the bloodstream can then flow into the liver, where it is converted into urea, which is 40 times less toxic than ammonia, through the ornithine cycle. Serum TP, composed of ALB and GLB, reflects the animal's immune capacity and is closely linked to protein metabolism. BUN is an important indicator of nitrogen content in animals. Its level is affected by protein (CP) intake and breakdown, accurately reflecting the level of protein catabolism and its impact on liver function in mammals. It is also related to BUN levels and rumen ammonia levels in mammals. In the above experiment of the present invention, there was no significant difference in serum TP, ALB, GLB, and BUN among the treated groups, indicating that replacing soybean meal in the diet with amino acid liquid fermented feed had no significant effect on the body's protein metabolism and nutritional level.

[0084] AST and ALT are two key transaminases in the body. They play a vital role in the protein metabolism process and are important indicators for measuring the healthy function of the liver. When abnormalities occur, AST and ALT in the serum will rise sharply. Existing studies have shown that both AST and ALT tend to decline after adding fermented feed. The reason is that fermented feed helps reduce inflammatory factors in the liver, thereby reducing the level of transaminases released into the blood. The present invention's study found that as the replacement ratio of amino acid liquid fermented feed increases, AST tends to decrease, and the AST in the T30 group is significantly lower than that in other control groups. There are no significant changes in ALT and ALP, indicating that the use of fermented amino acid liquid feed is more conducive to alleviating liver damage in fattening cattle. Another study found that fermented total mixed diets can significantly increase the triglycerides of Tibetan sheep. It is speculated that fermented feed can accelerate the absorption of fat by animals. In addition, the addition of yeast and Bacillus subtilis to the feed can increase the triglycerides of fattening cattle. The TG of the T10 group in the above experiment of the present invention was extremely significantly higher than that of the CON group. Triglycerides are the main form of energy storage in animals. Increased triglycerides are associated with improved growth performance of fattening cattle, indicating that fermented feed is more conducive to growth and production performance.

[0085] Regarding the effects of fermented feed replacing soybean meal at different ratios on the rumen microbiome of fattening cattle: Ruminant microbes are crucial to the digestive process of ruminants and are quite complex, primarily comprising bacteria, archaea, protozoa, and fungi. The species and abundance of rumen microbes are closely related to the animal breed, feed composition, and feeding environment. The Coverage Index for the different treatment groups in the present study exceeded 99%, demonstrating that the sequencing results accurately reflect the species and structural diversity of the rumen microbiome of fattening cattle. The Chao1 and Observed_species indices reflect community richness; higher values ​​indicate higher community richness; the Shannon and Simpson indices reflect species diversity; higher values ​​indicate higher community diversity. Beta diversity primarily analyzes the differences and similarities between microbial communities in different treatment groups. Principal coordinates analysis (PCoA) is generally used to examine differences between individuals or groups. The results of the above-mentioned experiments showed no differences in the alpha diversity index and beta diversity among the four treatments, indicating that replacing soybean meal at different ratios with fermented feed had no significant effect on the diversity of the rumen microbiome of fattening cattle. However, compared with the CON group, the number of observed species tended to decrease with the increase of the amount of fermented feed replacing soybean meal (0.05<P<0.1), suggesting that the use of amino acid fermented feed would reduce the number of species in the rumen to a certain extent and affect rumen fermentation.

[0086] In summary, the above-described embodiments and test results of the present invention demonstrate that the amino acid waste liquor fermented feed of the present invention has no negative effects in actual feeding of fattening cattle, and can improve growth performance and save costs. Furthermore, overall, replacing soybean meal with 20% amino acid fermented liquor in feed has the best performance and economic benefits for fattening cattle.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An amino acid liquid fermented feed, characterized in that: The amino acid liquid fermented feed is prepared by mixing and fermenting dried amino acid liquid and rice bran in a ratio of 1:

1.

2. The method for preparing an amino acid liquid fermented feed according to claim 1, characterized in that: The method comprises the following steps: mixing dried amino acid liquid and rice bran in a ratio of 1:1, wherein the water content of the mixture is controlled at 37.5%, the inoculation amount of microorganisms is 12.2%, the fermentation temperature is 30°C, and the fermentation is carried out for 15 days to prepare amino acid liquid fermented feed.

3. Use of the amino acid liquid fermented feed according to claim 1 or the preparation method of the amino acid liquid fermented feed according to claim 2 in preparing feed for fattening cattle.

4. The use according to claim 1, characterized in that The amino acid liquid fermented feed is used to replace soybean meal raw material.

5. The use according to claim 4, characterized in that The amino acid liquid fermented feed replaces soybean meal in an amount of 10%-30%.

6. The use according to claim 5, characterized in that The amino acid liquid fermented feed replaces soybean meal by 20%.

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