Sustained-release composition with function of assisting in regulating blood sugar and preparation method of sustained-release composition

Abstract

The invention discloses a slow-release composition with an auxiliary blood sugar regulation function and a preparation method thereof, and belongs to the technical field of functional foods. The composition carefully selects a plurality of natural sugar-resistant components, a composite fiber component and a slow-release auxiliary agent, wherein the natural sugar-resistant components comprise sugarcane polyphenol powder, a mulberry leaf extract, stevia rebaudiana polyphenol, a white kidney bean extract and lemon fruit concentrated powder; the composite fiber component is prepared from oat beta-glucan, citrus fiber and composite probiotics; the slow-release auxiliary agent is prepared from oxidized modified sodium alginate (SA), chitosan (CTS) and hydroxypropyl methyl cellulose (HPMC). By adjusting the ratio of the sugarcane polyphenol powder, the mulberry leaf extract, the white kidney bean extract and the like to the sodium alginate and the chitosan and combining a specific preparation process (such as a freeze-drying technology and the like), the slow release time of a sugar-resistant component in a body is regulated and controlled, the rising of postprandial blood sugar of a human body is controlled in cooperation with multiple channels of the composite fibers, and meanwhile the auxiliary weight reduction effect is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and in particular to a sustained-release composition with the function of assisting in regulating blood sugar and a preparation method thereof. Background Art

[0002] With the acceleration of the pace of life and changes in eating habits, the risk of chronic recurrence of obesity has increased due to excessive food intake and a sedentary lifestyle. Daily intake of high-fat, high-glycemic index foods can easily lead to a sharp increase in blood glucose concentration and insulin levels after meals. The sudden increase in insulin secretion may increase the risk of fat deposition by promoting lipid synthesis. In the current field of nutrition research, how to maintain metabolic homeostasis by regulating dietary blood glucose response while ensuring adequate nutrient intake and having a weight loss effect has become a core issue of common concern to academia and health consumers. The following problems still exist in the current field of nutrition research:

[0003] 1. The sugar-blocking components are released quickly, resulting in a short duration of sugar control and blood sugar metabolism regulation, requiring frequent consumption.

[0004] 2. Currently, most common functional foods for controlling blood sugar and reducing weight control energy intake by prolonging satiety, or add certain functional ingredients to inhibit blood sugar rise and carbohydrate absorption. The actual effect of controlling blood sugar and reducing weight is limited.

[0005] The sustained-release composition for assisting blood sugar regulation comprises a variety of natural glucose-blocking ingredients, a composite fiber component, and a sustained-release aid. The natural glucose-blocking ingredients include sugarcane polyphenol powder, mulberry leaf extract, and stevia polyphenols; the sustained-release aids include SA and CTS. These ingredients, combined with the composite fiber component (oat β-glucan, citrus fiber, and a composite probiotic), synergize to control postprandial blood sugar spikes while also achieving weight loss. Summary of the Invention

[0006] In order to solve the problems mentioned in the above background technology, the purpose of the present invention is to provide a sustained-release composition with the function of assisting in regulating blood sugar and a preparation method thereof. The composition can regulate the sustained-release time of the sugar-blocking component in the body, coordinate the composite fiber multi-pathway to control the rise of blood sugar in the human body after a meal, and at the same time have an auxiliary weight loss effect.

[0007] Composition: Natural sugar-blocking ingredients include sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenols, white kidney bean extract, etc.

[0008] Sustained-release adjuvants include oxidatively modified sodium alginate (SA), chitosan (CTS), and hydroxypropyl methylcellulose (HPMC).

[0009] The complex fiber components include oat beta-glucan, citrus fiber, complex probiotics, etc.

[0010] In order to achieve the above object, the present invention adopts the following technical solutions:

[0011] A sustained-release composition with the function of assisting in regulating blood sugar, comprising the following components in parts by weight:

[0012] 5-20 parts of sugarcane polyphenol powder, 10-30 parts of mulberry leaf extract, 4-10 parts of stevia polyphenols, 15-40 parts of white kidney bean extract, 2-8 parts of lemon fruit concentrate powder, 2-8 parts of oat beta-glucan, 0.3-3 parts of citrus fiber, 0.1-2 parts of compound probiotics, and 10-20 parts of sustained-release aids.

[0013] Preferably, the weight ratio of the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, lemon fruit concentrate powder, and sustained-release adjuvant is (7-15): (10-25): (4-7): (18-25): (2-6): (12-18).

[0014] Preferably, the weight ratio of the oat β-glucan to the citrus fiber is (4-8): (0.5-2).

[0015] The compound probiotics include Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020 and Lactobacillus plantarum.

[0016] Preferably, the weight ratio of the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, lemon fruit concentrate powder, and sustained-release adjuvant is 15:15:4:20:5:18.

[0017] Preferably, the weight ratio of the oat β-glucan to the citrus fiber is 3:1.

[0018] Preferably, the preparation is composed of the following components in parts by weight: 7-15 parts of sugarcane polyphenol powder, 10-25 parts of mulberry leaf extract, 4-7 parts of stevia polyphenols, 18-25 parts of white kidney bean extract, 2-6 parts of lemon fruit concentrate powder, 12-18 parts of sustained-release aid, 4-8 parts of oat beta-glucan, 0.3-2 parts of citrus fiber, and 1-2 parts of compound probiotics.

[0019] Preferably, the preparation is composed of the following components in parts by weight: 15 parts of sugarcane polyphenol powder, 25 parts of mulberry leaf extract, 6 parts of stevia polyphenols, 18 parts of white kidney bean extract, 18 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 8 parts of oat beta-glucan, 2 parts of citrus fiber, and 2 parts of compound probiotics.

[0020] Preferably, the number of viable bacteria of Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020 and Lactobacillus plantarum in the composite probiotics is 3*10 6 -2.5*10 10 cfu / g.

[0021] Preferably, the sustained-release adjuvant is composed of the following components in parts by weight: 6-10 parts of SA, 6-10 parts of CTS, and 3-5 parts of HPMC.

[0022] The method for preparing the sustained-release composition for assisting in regulating blood sugar comprises the following steps:

[0023] S1. Sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder are mixed in the aforementioned weight portions, ultrafinely ground to a particle size of ≤50 μm, and mixed with oxidatively modified SA and CTS in a weight ratio of (2-8):(2-5). A 1-3 ratio HPMC-50% ethanol solution is sprayed on the mixture, dried for 30-60 minutes, and citric acid is added to adjust the pH to 3.0-5.5. The temperature is controlled at 35-45°C, and freeze-dried to prepare particles to obtain a sustained-release complex.

[0024] S2. Add the oat β-glucan, citrus fiber, and compound probiotics in the prescribed amount to the complex obtained in step S1, and mix well to obtain the sustained-release composition for regulating blood sugar.

[0025] Modified polysaccharide composite carrier:

[0026] Oxidatively modified sodium alginate is prepared using a sodium periodate selective oxidation method. A 2% aqueous solution of sodium alginate is then added with 0.1-0.3 mol / L sodium periodate (molar ratio 1:0.2). The solution is then allowed to react in the dark for 24 hours. This reaction specifically cleaves the C2-C3 bond, generating oxidatively modified sodium alginate (OSA) with aldehyde groups. The aldehyde content can be controlled by the amount of oxidant (5-50%). The aldehyde groups of OSA can form Schiff base covalent bonds with the amino groups of chitosan, significantly enhancing the network stability. The modified gel exhibits a 40% reduction in swelling in simulated gastric fluid, while delayed release in intestinal fluid is ≥2 hours.

[0027] Chitosan-Carboxymethylated Dual Modification (N,O-CMCS): Chitosan is reacted with chloroacetic acid under alkaline conditions to introduce carboxymethyl groups at both the N and O positions. The reaction is controlled at pH 9, temperature 40-50°C, and time 5-6 hours, achieving a degree of substitution greater than 0.8. Carboxymethylation lowers the isoelectric point of chitosan to less than pH 5.0, ensuring solubility in gastric acid and preventing premature disintegration of the carrier. Furthermore, the added carboxyl groups enhance ionic crosslinking with sodium alginate.

[0028] The sustained-release complex microcapsule particles formed by the above process have a thin core-shell structure, the sugar-blocking component is in close contact with CTS / SA, and the outer layer of HPMC provides physical protection. In vitro tests show that the release rate of the microcapsule particles in simulated gastric fluid for 2 hours is <15%, and the release rate in intestinal fluid for 4 hours is 85%, showing an ideal biphasic release characteristic.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] 1. The composition of the present invention is compounded with natural sugar-blocking components and sustained-release adjuvants in a specific ratio. Through a specific preparation process, the sustained-release time of the sugar-blocking components in the body can be regulated. At the same time, the composite fiber multi-pathway controls the fluctuation of blood sugar after a meal, covering the peak period of blood sugar and the entire metabolic process, and plays an excellent role in controlling blood sugar. In addition, the composition provided by the present invention is targeted at overweight people and has a better weight loss effect.

[0031] 2. The sodium alginate (SA)-chitosan (CTS)-hydroxypropyl methylcellulose (HPMC) system constructs a multi-layered, multi-mechanism composite physical barrier system. SA cross-links with Ca2+ to form a primary stable gel core structure. CS forms a dense polyelectrolyte complex with SA, enhancing network strength and density. HPMC physically entangles and fills the network pores, forming a gel layer that extends the diffusion path. Furthermore, the pH-responsiveness of SA and CS, combined with the pH-independence of HPMC, synergizes under varying gastrointestinal pH conditions to optimize sustained-release behavior.

[0032] 3. Sugarcane Polyphenols Powder + White Kidney Bean Extract + Lemon Fruit Concentrate: Sugarcane polyphenols powder and white kidney bean extract contain α-amylase and α-glucosidase inhibitors, respectively, which prevent the breakdown of starch and disaccharides into small molecule sugars in the mouth and small intestine. The bioflavonoids in lemon fruit concentrate naturally increase endogenous GLP-1. The former and the latter reduce glucose production and absorption, while the latter further slows the digestion and absorption of carbohydrates in the small intestine by delaying gastric emptying, giving the former and the latter more time to work and reducing the amount of glucose that needs to be processed per unit time.

[0033] 4. Mulberry leaf extract (DNJ) + stevia polyphenols: Mulberry leaf extract contains the alkaloid DNJ (1-deoxynojirimycin), which has an inhibitory effect on the activity of disaccharide-degrading enzymes and can inhibit the absorption of disaccharides by the small intestine; its alkaloids fagomine and mulberry leaf polysaccharides can promote the secretion of insulin by β cells, and insulin can promote the cell's utilization of sugar and liver glycogen synthesis; stevia polyphenols enhance the activity of antioxidant enzymes through the Nrf2 / HO-1 pathway, protect pancreatic cells, and at the same time improve the glucose utilization rate of peripheral tissues; it plays a role in source blocking and terminal enhancement in blood sugar regulation.

[0034] 5. Oat β-glucan + citrus fiber: Oat β-glucan forms a viscous gel with water in the body, slowing gastric emptying while promoting GLP-1 secretion by L cells in the terminal ileum, enhancing insulin sensitivity, and inhibiting glucagon release. Citrus fiber increases chyme volume through its high water retention, prolonging intestinal transit time, and synergistically delaying glucose absorption with oat β-glucan.

[0035] Compound probiotics (such as Bifidobacterium lactis): regulate intestinal flora, control blood sugar homeostasis, focus on anti-inflammation and pancreatic islet protection, etc. Through the functional complementarity of strains, a full-pathway intervention covering "sugar absorption-insulin secretion-inflammation inhibition" is formed.

[0036] 6. The above compound components achieve multi-target coverage, acting respectively on digestive enzymes (amylase, glucosidase), gastrointestinal motility (gastric emptying), key incretin (GLP-1), and pancreatic hormones (insulin, glucagon), covering multiple key links from food intake to glucose utilization by cells, forming a more comprehensive blood sugar regulation network. DETAILED DESCRIPTION

[0037] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0038] Preparation Example 1

[0039] Slowly add 2.00g of sodium alginate to 98.00g of deionized water while stirring thoroughly. The stirring speed should not be too fast to avoid excessive bubbles. Continue stirring until the sodium alginate is completely dissolved to form a uniform solution.

[0040] In a light-proof environment, 0.1 mol / L sodium periodate solution was slowly added dropwise to a 2% sodium alginate aqueous solution. After the addition was completed, the reaction vessel was placed in a constant temperature water bath at 25°C, kept in the dark, and stirred at 200 rpm for 24 hours. After the reaction was carried out for 24 hours, 5 mL of ethylene glycol was added to the reaction solution and stirred for 15 minutes to terminate the oxidation reaction.

[0041] The reaction solution was transferred to a dialysis bag with a molecular weight cutoff of 8000Da and dialyzed with deionized water for 72 hours, with the deionized water replaced every 8 hours to remove impurities and small molecules in the reaction solution. After the dialysis was completed, the solution was freeze-dried to obtain a white to light yellow oxidatively modified sodium alginate solid.

[0042] Preparation Example 2

[0043] Disperse 5.00 g of chitosan in 100 mL of isopropanol and stir evenly. Slowly add 20 mL of an aqueous solution containing 10 g of NaOH in an ice-water bath and continue stirring for 30 minutes to fully alkalize the chitosan to obtain an alkaline chitosan solution.

[0044] Dissolve 16.35 g of chloroacetic acid in 50 mL of isopropanol and slowly add it dropwise to the alkaline chitosan solution. After the addition is complete, raise the reaction temperature to 45°C and stir at this constant temperature for 5 hours. During this time, use 10% NaOH solution to maintain pH = 9;

[0045] After the reaction, cool to room temperature and neutralize with glacial acetic acid to pH 7. Pour the reaction solution into 500 mL of anhydrous ethanol and stir to precipitate. Wash the precipitate three times with 80% ethanol and once with anhydrous ethanol to remove any remaining impurities. Dry under vacuum at 60°C to constant weight to obtain a white to light yellow carboxymethylated double-modified chitosan solid.

[0046] Example 1

[0047] A method for preparing a sustained-release composition having the function of assisting in regulating blood sugar comprises the following steps:

[0048] S1. Prepare the following by weight: 16 parts of sugarcane polyphenol powder, 24 parts of mulberry leaf extract, 6 parts of stevia polyphenols, 16 parts of white kidney bean extract, 15 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 3 parts of oat β-glucan, 3 parts of citrus fiber, and 1 part of compound probiotics;

[0049] The sustained-release adjuvant is composed of the following components in parts by weight: 7 parts of SA in Preparation Example 1, 7 parts of CTS in Preparation Example 2, and 4 parts of HPMC;

[0050] The number of viable bacteria in the composite probiotics is 2.5*10 10 cfu / g Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, Lactobacillus plantarum;

[0051] S2. After mixing the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder in the above formula, ultrafinely grind them to a particle size of ≤50 μm, mix them with 7 parts of oxidatively modified SA in Preparation Example 1 and 7 parts of CTS in Preparation Example 2, spray them with a 1-3 ratio of HPMC-50% ethanol solution, dry them for 45 minutes, add citric acid, adjust the pH to 3.0, control the temperature at 40°C, and use freeze-drying technology to prepare particles to obtain a sustained-release complex, which is evenly mixed with the oat β-glucan, citrus fiber, and composite probiotics in the above formula to obtain the sustained-release composition for regulating blood sugar.

[0052] Example 2

[0053] A method for preparing a sustained-release composition having the function of assisting in regulating blood sugar comprises the following steps:

[0054] S1. Prepare the following by weight: 5 parts of sugarcane polyphenol powder, 10 parts of mulberry leaf extract, 4 parts of stevia polyphenols, 15 parts of white kidney bean extract, 12 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 3 parts of oat β-glucan, 2 parts of citrus fiber, and 1 part of compound probiotics;

[0055] The sustained-release adjuvant is composed of the following components in parts by weight: 6 parts of SA in Preparation Example 1, 7 parts of CTS in Preparation Example 2, and 3 parts of HPMC;

[0056] The number of viable bacteria in the composite probiotics is 9*10 7 cfu / g Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, Lactobacillus plantarum;

[0057] S2. After mixing the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder in the above formula, ultrafinely grind them to a particle size of ≤50 μm, mix them with 6 parts of oxidatively modified SA in Preparation Example 1 and 7 parts of CTS in Preparation Example 2, spray them with a 1-3 ratio of HPMC-50% ethanol solution, dry them for 30 minutes, add citric acid, adjust the pH to 5.5, control the temperature at 45°C, and use freeze-drying technology to prepare particles to obtain a sustained-release complex, which is evenly mixed with the oat β-glucan, citrus fiber, and composite probiotics in the above formula to obtain the sustained-release composition for regulating blood sugar.

[0058] Example 3

[0059] A method for preparing a sustained-release composition having the function of assisting in regulating blood sugar comprises the following steps:

[0060] S1. Prepare the following by weight: 18 parts of sugarcane polyphenol powder, 25 parts of mulberry leaf extract, 9 parts of stevia polyphenols, 16 parts of white kidney bean extract, 15 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 4 parts of oat β-glucan, 1 part of citrus fiber, and 1 part of compound probiotics;

[0061] The sustained-release adjuvant is composed of the following components in parts by weight: 8 parts of SA in Preparation Example 1, 8 parts of CTS in Preparation Example 2, and 4 parts of HPMC;

[0062] The number of viable bacteria in the composite probiotics is 1.5*10 10 cfu / g Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, Lactobacillus plantarum;

[0063] S2. After mixing the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder in the above formula, ultrafinely grind them to a particle size of ≤50 μm, mix them with 8 parts of oxidatively modified SA in Preparation Example 1 and 8 parts of CTS in Preparation Example 2, spray them with a 1-3 ratio of HPMC-50% ethanol solution, dry them for 50 minutes, add citric acid, adjust the pH to 4.0, control the temperature at 40°C, and use freeze-drying technology to prepare particles to obtain a sustained-release complex, which is evenly mixed with the oat β-glucan, citrus fiber, and composite probiotics in the above formula to obtain the sustained-release composition for regulating blood sugar.

[0064] Example 4

[0065] A method for preparing a sustained-release composition having the function of assisting in regulating blood sugar comprises the following steps:

[0066] S1. Prepare the following by weight: 18 parts of sugarcane polyphenol powder, 26 parts of mulberry leaf extract, 8 parts of stevia polyphenols, 17 parts of white kidney bean extract, 18 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 3 parts of oat β-glucan, 1 part of citrus fiber, and 1 part of compound probiotics;

[0067] The sustained-release adjuvant is composed of the following components in parts by weight: 9 parts of SA in Preparation Example 1, 7 parts of CTS in Preparation Example 2, and 5 parts of HPMC;

[0068] The number of viable bacteria in the composite probiotics is 3*10 8 cfu / g Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, Lactobacillus plantarum;

[0069] S2. After mixing the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder in the above formula, ultrafinely grind them to a particle size of ≤50 μm, mix them with 9 parts of oxidatively modified SA in Preparation Example 1 and 7 parts of CTS in Preparation Example 2, spray them with a 1-3 ratio of HPMC-50% ethanol solution, dry them for 60 minutes, add citric acid, adjust the pH to 3.0, control the temperature at 45°C, and use freeze-drying technology to prepare particles to obtain a sustained-release complex, which is evenly mixed with the oat β-glucan, citrus fiber, and composite probiotics in the above formula to obtain the sustained-release composition for regulating blood sugar.

[0070] Example 5

[0071] A method for preparing a sustained-release composition having the function of assisting in regulating blood sugar comprises the following steps:

[0072] S1. Prepare the following by weight: 19 parts of sugarcane polyphenol powder, 25 parts of mulberry leaf extract, 8 parts of stevia polyphenols, 30 parts of white kidney bean extract, 18 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 3 parts of oat β-glucan, 2 parts of citrus fiber, and 1.5 parts of compound probiotics;

[0073] The sustained-release adjuvant is composed of the following components in parts by weight: 7 parts of SA in Preparation Example 1, 9 parts of CTS in Preparation Example 2, and 4 parts of HPMC;

[0074] The number of viable bacteria in the composite probiotics is 2.5*10 9 cfu / g Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, Lactobacillus plantarum;

[0075] S2. After mixing the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder in the above formula, ultrafinely grind them to a particle size of ≤50 μm, mix them with 7 parts of oxidatively modified SA in Preparation Example 1 and 9 parts of CTS in Preparation Example 2, spray them with a 1-3 ratio of HPMC-50% ethanol solution, dry them for 45 minutes, add citric acid, adjust the pH to 4.5, control the temperature at 45°C, and use freeze-drying technology to prepare particles to obtain a sustained-release complex, which is evenly mixed with the oat β-glucan, citrus fiber, and composite probiotics in the above formula to obtain the sustained-release composition for regulating blood sugar.

[0076] Example 6

[0077] A method for preparing a sustained-release composition having the function of assisting in regulating blood sugar comprises the following steps:

[0078] S1. Prepare the following by weight: 12 parts of sugarcane polyphenol powder, 15 parts of mulberry leaf extract, 7 parts of stevia polyphenols, 16 parts of white kidney bean extract, 18 parts of sustained-release aid, 6 parts of lemon fruit concentrate powder, 3 parts of oat β-glucan, 1 part of citrus fiber, and 1.5 parts of compound probiotics;

[0079] The sustained-release adjuvant is composed of the following components in parts by weight: 8 parts of SA in Preparation Example 1, 7 parts of CTS in Preparation Example 2, and 5 parts of HPMC;

[0080] The number of viable bacteria in the composite probiotics is 1.5*10 9 cfu / g Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, Lactobacillus plantarum;

[0081] S2. After mixing the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder in the above formula, ultrafinely grind them to a particle size of ≤50 μm, mix them with 8 parts of oxidatively modified SA in Preparation Example 1 and 7 parts of CTS in Preparation Example 2, spray them with a 1-3 ratio of HPMC-50% ethanol solution, dry them for 60 minutes, add citric acid, adjust the pH to 5.5, control the temperature at 45°C, and use freeze-drying technology to prepare particles to obtain a sustained-release complex, which is evenly mixed with the oat β-glucan, citrus fiber, and composite probiotics in the above formula to obtain the sustained-release composition for regulating blood sugar.

[0082] Comparative Example 1

[0083] The difference between this comparative example and Example 1 is that the sustained-release adjuvant is composed of the following components in parts by weight: 7 parts of SA in Preparation Example 1 and 4 parts of HPMC.

[0084] Comparative Example 2

[0085] The difference between this comparative example and Example 1 is:

[0086] The sustained-release adjuvant is composed of the following components in parts by weight: 7 parts of CTS in Preparation Example 2 and 4 parts of HPMC.

[0087] Comparative Example 3

[0088] The difference between this comparative example and Example 1 is that the sustained-release adjuvant is composed of the following components in parts by weight: 7 parts of common commercially available SA, 7 parts of CTS in Preparation Example 2, and 4 parts of HPMC. SA is purchased from Anhui Weimao Biotechnology Co., Ltd. and has a CAS number of 9005-38-3.

[0089] Comparative Example 4

[0090] The difference between this comparative example and Example 1 is that the sustained-release adjuvant is composed of the following components in parts by weight: 7 parts of SA in Preparation Example 1, 7 parts of common commercially available CTS, and 4 parts of HPMC. CTS is purchased from Henan Bangrun Chemical Products Co., Ltd. and has a CAS number of 9012-76-4.

[0091] Comparative Example 5

[0092] The difference between this comparative example and Example 1 is that: the sustained-release aid is composed of the following components in parts by weight: ordinary commercially available SA 7 parts, ordinary commercially available CTS 7 parts, HPMC 4 parts;

[0093] SA was purchased from Anhui Weimao Biotechnology Co., Ltd., CAS No.: 9005-38-3;

[0094] CTS was purchased from Henan Bangrun Chemical Products Co., Ltd., with the CAS number being 9012-76-4.

[0095] Experimental Example 1 (Delayed Blood Glucose Level)

[0096] Test Method

[0097] Twelve healthy adults were selected for each group, including 6 men and 6 women, aged 23-35 years, with a BMI of 18.8-23.0 kg / m 2 , no history of diabetes (or impaired glucose tolerance), no other metabolic diseases, digestive system diseases, endocrine system diseases and mental illnesses, etc.; no history of allergy or intolerance to the food to be tested, normal oral glucose tolerance test, regular diet, no recent gastrointestinal diseases or taking any medications, and passed the physical examination. Before the experiment, the subjects were trained and signed an informed consent form. Stop eating and drinking after 22:00 the night before the experiment, and monitor postprandial blood sugar fluctuations. The control group: ate the reference food white bread for breakfast;

[0098] Experimental group: Consume one portion of the composition provided in each embodiment and comparative example within 30 minutes before breakfast;

[0099] Comparison was made between the consumption and non-consumption of the compositions of the embodiments of the present invention. A blood glucose response curve was prepared with time as the abscissa and the blood glucose value minus the fasting blood glucose value at each time point as the ordinate. The area under the curve (IAUC) above the fasting blood glucose level was calculated. The inhibition rate = (reference food IAUC - example IAUC) / reference food IAUC + 100%.

[0100] Blood glucose test uses Roche blood glucose meter, the specific operation of measuring fingertip blood glucose is as follows:

[0101] (1) Collect blood from the fingertip, discard the first drop, and measure fasting blood glucose twice with an interval of 5 min and record the results;

[0102] (2) Consume 50 mL of water;

[0103] (3) 5 minutes later, eat a slice of white bread (calculated as 50 g carbohydrate intake) and 200 mL of water (consume within 5 minutes);

[0104] (4) Collect blood from fingertips to test blood glucose and record the results (15 min, 30 min, 45 min, 60 min, 90 min, and 120 min after a meal).

[0105] The average inhibition rates of the examples and comparative examples were statistically analyzed, and the results are shown in Table 1:

[0106] Table 1

[0107] Test items Average inhibition rate (%) Example 1 65% Example 2 63% Example 3 62% Example 4 64% Example 5 65% Example 6 61% Comparative Example 1 38% Comparative Example 2 35% Comparative Example 3 45% Comparative Example 4 42% Comparative Example 5 38% control group 5%

[0108] Experimental Example 2 (Weight Loss Effect)

[0109] Test Method

[0110] Volunteer experiment: Each group selected 10 healthy adult males, aged 23-35 years old, with a BMI range of 25-30 kg / m 2 The lipid content ranged from 23% to 35%, and the volunteers had no history of diabetes, other metabolic diseases, digestive system diseases, endocrine system diseases, and neurologic diseases. They had a regular diet and had no recent gastrointestinal diseases or medications. Volunteers were trained before the experiment. A unified diet was maintained for one week before the experiment, and the control group followed a unified diet. The experimental groups consumed the compositions prepared in Examples 1 to 6 and Comparative Examples 1 to 5, respectively. The experimental period was 4 weeks, and they followed a unified diet. At lunch, 10 g of the composition was taken by diluting it with water and drinking it within 10 minutes.

[0111] The weight and body fat changes of each embodiment and comparative example were statistically analyzed, and the results are shown in Table 2:

[0112] Table 2

[0113] Test items Body weight change (% change from day 1 relative to vehicle) Body fat change (% change from day 1 relative to vehicle) Example 1 25% 45% Example 2 23% 46% Example 3 22% 45% Example 4 24% 43% Example 5 25% 45% Example 6 21% 47% Comparative Example 1 13% 28% Comparative Example 2 15% 25% Comparative Example 3 18% 32% Comparative Example 4 19% 31% Comparative Example 5 9% 18% control group 0.5% 1.5%

[0114] In summary: the sustained-release composition prepared in Examples 1-6 of the present invention can realize the regulation of the sustained-release time of the sugar-blocking component in the body; at the same time, the composite fiber multi-pathway controls the fluctuation of blood sugar after a meal in the human body, covers the peak period of blood sugar and the entire metabolic process, and plays an excellent role in controlling blood sugar; in addition, the composition provided by the present invention is targeted at overweight people and has a better weight loss effect.

[0115] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A sustained-release composition with the function of assisting in regulating blood sugar, characterized in that: The composition is as follows in parts by weight: 5-20 parts of sugarcane polyphenol powder, 10-30 parts of mulberry leaf extract, 4-10 parts of stevia polyphenols, 15-40 parts of white kidney bean extract, 2-8 parts of lemon fruit concentrate powder, 2-8 parts of oat beta-glucan, 0.3-3 parts of citrus fiber, 0.1-2 parts of compound probiotics, and 10-20 parts of sustained-release aids.

2. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 1, characterized in that: The weight ratio of the sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, lemon fruit concentrated powder and sustained-release adjuvant is (7-15): (10-25): (4-7): (18-25): (2-6): (12-18).

3. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 1, characterized in that: The weight ratio of the oat beta-glucan to the citrus fiber is (4-8): (0.5-2).

4. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 1, characterized in that: The compound probiotics include Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020 and Lactobacillus plantarum.

5. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 1, characterized in that: The number of viable bacteria in the composite probiotics, including Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus rhamnosus GM-020, and Lactobacillus plantarum, is 3*10 6 -2.5*10 10 cfu / g.

6. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 1, characterized in that: The sustained-release adjuvant comprises oxidatively modified sodium alginate (SA), carboxymethylated double-modified chitosan (CTS), and hydroxypropyl methylcellulose (HPMC).

7. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 6, characterized in that: The sustained-release adjuvant is composed of the following components in parts by weight: 6-10 parts of SA, 6-10 parts of CTS, and 3-5 parts of HPMC.

8. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 6, characterized in that: The oxidative modification of sodium alginate adopts a sodium periodate selective oxidation method, which specifically includes the following steps: Prepare a 2% aqueous solution of sodium alginate, add 0.1-0.3 mol / L sodium periodate solution at a molar ratio of 1:0.2, and react in the dark for 20-24 hours.

9. The sustained-release composition having the function of assisting in regulating blood sugar according to claim 1, characterized in that: The preparation method of the carboxymethylated double-modified chitosan comprises the following steps: Chitosan reacts with chloroacetic acid under alkaline conditions, and carboxymethyl groups are introduced at the N and O positions. The reaction pH is controlled at 9, the temperature is 40-50°C, the time is 5-6h, and the degree of substitution reaches >0.

8.

10. A method for preparing the sustained-release composition having the function of assisting in regulating blood sugar according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. After mixing sugarcane polyphenol powder, mulberry leaf extract, stevia polyphenol, white kidney bean extract, and lemon fruit concentrate powder according to the weight parts, ultrafine grinding is performed to a particle size of ≤50 μm, and mixing with the weight parts of oxidatively modified SA and CTS, spraying the weight parts of HPMC-50% ethanol solution, drying for 30-60 minutes, adding citric acid to adjust the pH to 3.0-5.5, controlling the temperature at 35-45° C., and using freeze-drying technology to prepare particles to obtain a sustained-release complex; S2. Add the oat β-glucan, citrus fiber, and compound probiotics in the prescribed amount to the complex obtained in step S1, and mix well to obtain the sustained-release composition for regulating blood sugar.