Application of traditional Chinese medicine composition in preparation of medicine for relieving myelosuppression after chemotherapy

Abstract

The invention provides application of a traditional Chinese medicine composition in preparation of a medicine for relieving myelosuppression after chemotherapy, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises 18-30 parts of deer heart blood powder, 10-15 parts of ginseng, 4-10 parts of radix polygonati officinalis, 4-10 parts of semen ziziphi spinosae, 18-25 parts of semen coicis, 4-10 parts of fructus citri, 8-15 parts of poria cocos and 8-15 parts of radix puerariae, can relieve the bone marrow injury form after chemotherapy, promotes peripheral blood circulation and immune cell recovery, and shows the effect of relieving bone marrow suppression after chemotherapy. The traditional Chinese medicine composition is free of toxic and side effects, easy to absorb, high in safety after long-term use and free of obvious drug resistance, and has a good prospect in preparation of drugs for conditioning bone marrow functions after chemotherapy.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to an application of a traditional Chinese medicine composition in preparing a medicine for alleviating bone marrow suppression after chemotherapy. Background Art

[0002] Chemotherapy, also often referred to as chemotherapy, is a therapeutic strategy used to kill tumor cells. It works by inhibiting tumor growth or eliminating tumors through the systemic or partial use of chemicals. Chemotherapy targets all cells in the human body, including tumor cells and normal cells. Although it has a significant effect in eliminating cancer cells, it also causes considerable damage to the body's normal cells, especially bone marrow cells. Bone marrow suppression is one of the common side effects of chemotherapy, which is manifested as a decline in bone marrow hematopoietic function and a significant decrease in the number of peripheral blood cells such as white blood cells, red blood cells and platelets, leading to adverse consequences such as decreased immunity, increased risk of infection, and anemia. Therefore, how to effectively regulate bone marrow function and promote the recovery of the immune system after chemotherapy has become a clinical problem that needs to be solved urgently.

[0003] Traditional Chinese Medicine (TCM) is gaining increasing recognition for its role in disease prevention. Its multi-component, multi-target nature gives it unique potential for the treatment and recovery of numerous illnesses. Numerous TCM formulas have been reported to exhibit synergistic effects in the treatment of major diseases and play a central role in recovery. Therefore, the search for effective therapeutics for post-chemotherapy bone marrow function, from a TCM perspective, holds great promise. Summary of the Invention

[0004] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a Chinese medicine composition for use in the preparation of a drug for alleviating bone marrow suppression after chemotherapy. The Chinese medicine composition can alleviate the morphology of bone marrow damage after chemotherapy, promote peripheral blood circulation and immune cell recovery, and show the efficacy of alleviating bone marrow suppression after chemotherapy.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The present invention provides an application of a traditional Chinese medicine composition in preparing a medicine for alleviating bone marrow suppression after chemotherapy. The traditional Chinese medicine composition comprises, by weight, 18 to 30 parts of deer heart blood powder, 10 to 15 parts of ginseng, 4 to 10 parts of polygonatum, 4 to 10 parts of spinach seeds, 18 to 25 parts of coix seeds, 4 to 10 parts of citron, 8 to 15 parts of poria cocos, and 8 to 15 parts of kudzu root.

[0007] Preferably, the Chinese medicine composition comprises, by mass, 30 parts of deer heart blood powder, 12 parts of ginseng, 6 parts of polygonatum, 6 parts of jujube seeds, 20 parts of coix seeds, 6 parts of citron, 10 parts of poria cocos and 10 parts of kudzu root.

[0008] Preferably, the Chinese medicine composition improves bone marrow damage caused by chemotherapy.

[0009] Preferably, the Chinese medicine composition reduces bone marrow cell vacuolation.

[0010] Preferably, the Chinese medicine composition improves erythropenia caused by chemotherapy.

[0011] Preferably, the Chinese medicine composition improves lymphocytopenia induced by chemotherapy.

[0012] Preferably, the lymphocytes include T cells, B cells and NK cells.

[0013] Preferably, the preparation method of the traditional Chinese medicine composition comprises the following steps:

[0014] Ginseng, polygonatum, jujube seeds, coix seeds, citron, poria and kudzu root are mixed, the obtained mixture is decocted with water, and the decoction is concentrated to 1 / 3 of the original volume to obtain a medicinal material extract; the medicinal material extract is freeze-dried to obtain a medicinal material extract freeze-dried powder, which is then mixed with deer heart blood powder and sterilized to obtain the traditional Chinese medicine composition.

[0015] Preferably, in the medicine, the content of the traditional Chinese medicine composition is 60 to 100 wt%.

[0016] Preferably, the dosage forms of the drug include capsules, oral liquids, granules, tablets and pills.

[0017] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0018] The present invention uses a Chinese medicine composition composed of Chinese medicinal raw materials with medicinal and edible properties to prepare a drug for alleviating bone marrow suppression after chemotherapy. The Chinese medicine composition, by virtue of its own multi-component and multi-target characteristics, can improve the morphology of bone marrow damage after chemotherapy, promote peripheral blood circulation and immune cell recovery, and show the efficacy of alleviating bone marrow suppression after chemotherapy. In addition, the Chinese medicine composition is systemically regulated, does not rely on human blood products or hormone drugs, has no toxic side effects, is easily absorbed, has high safety for long-term use, and does not have obvious drug resistance. It can be used as a dietary supplement or health food raw material to alleviate bone marrow damage after chemotherapy, promote the recovery of immune system damage, and alleviate bone marrow suppression after chemotherapy, and is suitable for promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 : Color comparison of femur and tibia of mice in each group;

[0020] Figure 2 : HE staining of femurs of mice in each group;

[0021] Figure 3 : Red blood cell counts in peripheral blood of mice in each group;

[0022] Figure 4 : Lymphocyte counts in peripheral blood of mice in each group;

[0023] Figure 5 : The proportion of various types of lymphocytes in the peripheral blood of mice in each group. DETAILED DESCRIPTION

[0024] The present invention provides a use of a traditional Chinese medicine composition in preparing a drug for alleviating post-chemotherapy bone marrow suppression, the traditional Chinese medicine composition comprising, by weight, 18-30 parts of deer heart blood powder, 10-15 parts of ginseng, 4-10 parts of polygonatum, 4-10 parts of jujube seeds, 18-25 parts of coix seeds, 4-10 parts of citron, 8-15 parts of poria, and 8-15 parts of kudzu root. Preferably, the traditional Chinese medicine composition comprises, by weight, 30 parts of deer heart blood powder, 12 parts of ginseng, 6 parts of polygonatum, 6 parts of jujube seeds, 20 parts of coix seeds, 6 parts of citron, 10 parts of poria, and 10 parts of kudzu root.

[0025] Deer heart blood is drawn from the heart of a slaughtered sika deer or red deer. Deer heart blood powder is made from this raw material. Modern clinical research shows that deer heart blood can treat palpitations, insomnia, forgetfulness, falls, and anti-aging. It can also be used to treat rheumatism and rheumatoid arthritis.

[0026] Ginseng is the root of Panax ginseng (Camey), a plant of the Araliaceae family. It has a sweet, slightly bitter flavor and a slightly warm nature. It enters the lung, spleen, and heart meridians. It has the effects of tonifying vital energy, tonifying the spleen and lungs, promoting fluid production, and calming the mind and improving intelligence. It is primarily used to treat qi deficiency with impatience, weak pulse, spleen deficiency, sinking of qi in the middle, wheezing and coughing due to lung deficiency, shortness of breath and fatigue, thirst due to loss of fluid, polydipsia due to deficiency-heat, insomnia and forgetfulness, palpitations, blood deficiency, chlorosis, impotence, and cold uterus.

[0027] Polygonatum odoratum (Mill.) Druce is the rhizome of Polygonatum odoratum (Mill.) Druce, a plant of the Liliaceae family. It is sweet and neutral in nature, entering the Lung and Stomach meridians. It nourishes yin and moistens dryness, promotes fluid production and quenches thirst. It is primarily used to treat dry-heat cough, thirst due to loss of fluid, yin deficiency caused by external infection, headache, and fever.

[0028] Ziziphus jujuba seeds are the mature seeds of Ziziphus jujuba Mill. var. spinosa (Bunge) Huex H.F. Chou, a plant of the Rhamnaceae family. They are neutral in nature, sweet and sour in flavor. They enter the Liver, Gallbladder, and Heart meridians. They nourish the liver, calm the mind, astringe sweat, and promote fluid production. They belong to the category of tranquilizers, a type of heart-nourishing and tranquilizing medicine. They are used to treat insomnia, palpitations, excessive dreams, excessive sweating due to physical weakness, and thirst caused by loss of body fluids.

[0029] Job's tears, the mature seed kernel of Coix lacryma-jobi L. var. mayuen (Roman.) Stapf, a plant of the Poaceae family, is cool in nature, with a sweet, bland flavor. It enters the spleen, stomach, and lung meridians. It strengthens the spleen and eliminates dampness, relieves numbness and stops diarrhea, and clears heat and discharges pus. It belongs to the diuretic and stranguria-relieving category of diuretics. It is used to treat edema caused by water retention, dysuria, stranguria, beriberi-induced edema, spleen deficiency diarrhea, dampness-induced stiffness and muscle spasms, difficulty flexing and extending muscles, or weakness, lung abscesses, coughing up pus and sputum, and intestinal abscesses.

[0030] Citron is the mature fruit of Citrus wilsonii Tanaka. (also known as Citrus medic L.) in the Rutaceae family. It is warm in nature, with a pungent, bitter, and sour flavor. It enters the Liver, Spleen, and Lung meridians. It soothes the liver, regulates Qi, relieves fullness, and resolves phlegm. It is a Qi-regulating herb used to treat liver-stomach Qi stagnation, chest and rib pain, abdominal distension, vomiting, belching, and cough with excessive phlegm.

[0031] Poria cocos, the sclerotium of the Polyporaceae fungus Poria cocos (Schw.) Wolf., is neutral in nature, with a sweet and bland taste. It enters the Heart, Lung, Spleen, and Kidney meridians. It promotes diuresis and eliminates dampness, strengthens the spleen, and calms the mind. It belongs to the diuretic and anti-edema category of diuretics and dampness-eliminating drugs. It is used to treat edema, oliguria, phlegm and fluid retention, dizziness and palpitations, spleen deficiency, poor appetite, loose stools and diarrhea, restlessness, palpitations, and insomnia.

[0032] Pueraria root is the dried root of Pueraria lobata (Willd.) Ohwi, a plant of the genus Pueraria in the Leguminosae family. It has a sweet and pungent flavor and a cooling nature. It enters the spleen and stomach meridians. It relieves muscle tension, reduces fever, clears rashes, promotes fluid production and quenches thirst, and promotes yang and relieves diarrhea. It is primarily used to treat superficial symptoms of fever, stiff neck and back pain, measles that has not cleared up, thirst caused by febrile illness, polydipsia due to yin deficiency, febrile diarrhea due to heatiness, and diarrhea due to spleen deficiency.

[0033] The Chinese medicine composition of the present invention uses deer heart blood powder and ginseng as main drugs (monarch drugs), which can greatly replenish deficiency, restore pulse and strengthen deficiency, benefit essence and blood, and strengthen tendons and bones; Polygonatum odoratum and Ziziphus jujuba seeds as assistant drugs, which can nourish yin and moisten dryness, promote the production of body fluid and quench thirst, and calm the mind and tranquilize the spirit; Coix lachryma-jobi, Citron, Poria cocos and Pueraria root can invigorate the spleen and eliminate dampness, regulate qi and harmonize the middle, soothe the liver and relieve depression. The whole prescription has the effects of invigorating qi and blood, calming the mind and tranquilizing the spirit, invigorating spleen and eliminating dampness, soothing the liver and relieving depression, and is suitable for people with deficiency syndromes such as deficiency of yin and yang, deficiency of qi and blood, deficiency of spleen and stomach qi and blood deficiency. The prescription of the Chinese medicine composition of the present invention has the characteristics of multiple components and multiple targets, can play the effects of invigorating qi and blood, regulating qi and harmonizing the middle, invigorating spleen and eliminating dampness, can be applied to the side effects caused by chemotherapy, can improve the morphology of bone marrow damage after chemotherapy, promote peripheral blood circulation and immune cell recovery, and show the effect of alleviating bone marrow suppression after chemotherapy.

[0034] The present invention has been verified by mouse experiments and found that the Chinese medicine composition prescription can restore the normal physiological state of the femur and tibia after chemotherapy. The color of the femur and tibia is close to normal dark pink, and the bone marrow cell vacuolation phenomenon in the femur is greatly reduced. The bone marrow cells are restored to a dense and uniform arrangement state, which effectively improves the bone marrow damage after chemotherapy; the Chinese medicine composition prescription can increase the number of red blood cells and lymphocytes (T cells, B cells, NK cells) in the peripheral blood after chemotherapy damage, promote peripheral blood circulation and immune cell recovery. In summary, the Chinese medicine composition prescription of the present invention shows the effect of alleviating bone marrow suppression after chemotherapy. The chemotherapy of the present invention is preferably cyclophosphamide administration treatment.

[0035] The preparation method of the traditional Chinese medicine composition of the present invention comprises the following steps: mixing ginseng, polygonatum, jujube seed, coix seed, citron, poria and kudzu root, decocting the obtained mixture with water, and concentrating the decoction to 1 / 3 of the original volume to obtain a medicinal material extract; freeze-drying the medicinal material extract to obtain a medicinal material extract freeze-dried powder, mixing the freeze-dried powder with deer heart blood powder, and sterilizing to obtain the traditional Chinese medicine composition.

[0036] The amount of water added for decoction according to the present invention is 8 to 10 times the mass of the mixture, preferably 9 times.

[0037] The decoction is preferably performed twice, more preferably for 1 to 2 hours each time. Alternatively, ginseng, polygonatum, jujube seeds, coix seeds, citron, poria, and kudzu root are mixed in proportion, the mixture is decocted with water, filtered to obtain a decoction, the residue is again decocted with an equal amount of water, filtered again to obtain a decoction, and the decoctions are combined.

[0038] The present invention concentrates the decoction to 1 / 3 of the original volume, which can make the interaction between the effective ingredients more stable and reduce the denaturation or precipitation of the ingredients caused by excessive concentration.

[0039] The medicinal material extract of the present invention is preferably freeze-dried at -40°C.

[0040] The deer heart blood powder of the present invention is preferably prepared by drying deer heart blood. As an optional embodiment, the present invention uses a freeze dryer to dry the deer heart blood stock solution to prepare the deer heart blood powder; the freeze drying is preferably performed at -40°C. As another optional embodiment, the present invention mixes the deer heart blood stock solution with a protective agent to form a uniform liquid, sprays it into a drying tower at a certain pressure (2-3 MPa), and controls the inlet air temperature at 180-200°C to quickly dry the deer heart blood into powder; the protective agent and deer heart blood are preferably mixed at a mass (g) / volume (mL) ratio of 1% to 3% to form a uniform liquid, and the protective agent includes mannitol, trehalose, and sucrose.

[0041] The present invention does not limit the specific sterilization method.

[0042] In the medicine of the present invention, the content of the Chinese medicine composition is 60-100wt%, preferably 80-98wt%. The medicine of the present invention may further include medically acceptable carrier excipients to ensure that the prepared preparation is convenient for preparation and clinical application. Further preferably, the excipients of the present invention include but are not limited to at least one of a diluent, a wetting agent, a binder, a lubricant, a colorant, and a coating agent.

[0043] The dosage forms of the medicine described in the present invention include capsules, oral liquids, granules, tablets, and pills. The dosage forms of the medicine described in the present invention are not limited thereto, and other achievable dosage forms are within the scope of protection of the present invention. As an optional embodiment, the present invention accurately proportions the processed plant medicinal material extract, deer heart blood powder, and excipients (such as maltodextrin, microcrystalline cellulose, magnesium stearate, etc.) according to the formula requirements. A V-shaped mixer is required during the mixing process to ensure that the raw materials and excipients are evenly distributed; the mixed raw materials are granulated and granulated using a one-step granulation drying device; the prepared granules are dried to remove excess moisture (the temperature needs to be controlled below 80°C during the drying process to avoid deformation or cracking of the granules, and a hot air circulation oven is used for drying); the dried granules are sterilized to kill the microorganisms and bacteria therein to obtain granules. As another optional embodiment, the present invention fills the sterilized granules into the capsule shell; hard capsule filling equipment is required during the filling process, and the dosage and weight difference of the capsules need to be controlled to ensure the accuracy and consistency of the product.

[0044] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0045] The experimental data were processed using SPSS 21.0 software and expressed as x ± s. P < 0.05 indicated a significant difference, P < 0.01 indicated a relatively significant difference, and P < 0.001 indicated an extremely significant difference.

[0046] In the following examples, unless otherwise specified, all methods are conventional.

[0047] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0048] Example 1

[0049] Forty-eight 8-10-week-old C57BL / 6 mice were provided by the Animal Research Center, Faculty of Health Sciences, University of Macau. The mice were randomly divided into six groups of eight: a blank control group (Control), a model control group (Model), a positive control group (Positive), and three experimental groups treated with different concentrations of the traditional Chinese medicine composition (low, medium, and high dose groups: DBF-L, DBF-M, and DBF-H).

[0050] The Chinese medicinal composition used in the experiment consists of (parts by weight): 30 parts of deer heart blood powder, 12 parts of ginseng, 6 parts of polygonatum, 6 parts of jujube seeds, 20 parts of coix, 6 parts of citron, 10 parts of poria, and 10 parts of kudzu root. The preparation method is as follows: ginseng, polygonatum, jujube seeds, coix, citron, poria, and kudzu root are mixed, the mixture is decocted with 9 times the weight of water, and filtered to obtain a decoction. The residue is again decocted with an equal amount of water, and filtered again to obtain a decoction. The decoctions are combined; the decoction is concentrated to 1 / 3 of its original volume to obtain a medicinal material extract; the deer heart blood stock solution is freeze-dried using a freeze dryer (-40°C) to obtain deer heart blood powder; the medicinal material extract and deer heart blood powder are mixed and sterilized to obtain the Chinese medicinal composition.

[0051] The Chinese herbal composition and positive control drug used in the experimental groups were dissolved in PBS. Mice were gavaged with 0.25 ml of the corresponding drug for 14 consecutive days. The blank and model control groups were gavaged with PBS daily, while the positive control group was gavaged with 300 mg / kg of caffeic acid daily. The low, medium, and high dose groups of the Chinese herbal composition were gavaged with 250 mg / kg, 500 mg / kg, and 1000 mg / kg of the Chinese herbal composition daily, respectively.

[0052] One week after gavage, all mice in the blank control group received daily intraperitoneal injections of 100 mg / kg of cyclophosphamide to induce myelosuppression for three consecutive days (Days 8, 9, and 10). The experiment ended on Day 15, at which point blood, femurs, and tibias were collected for testing.

[0053] At the end of the experiment, the femur and tibia of the mice were collected and their color changes were observed. Figure 1 The results showed that the femurs and tibias of mice in the blank control group were dark red, a normal physiological state; while the femurs and tibias of mice in the model control group were abnormally light pink after chemotherapy. The femurs and tibias of mice treated with the positive drug and the Chinese herbal composition returned to a near-normal dark pink color, indicating that the Chinese herbal composition, like the positive drug, has a positive effect in ameliorating bone marrow damage after chemotherapy.

[0054] The femur of one side of the mouse was fixed, decalcified, paraffin-embedded and sliced, and HE staining was performed. The effects of each group on the bone marrow morphology in the femur of the mouse were observed. The results are as follows Figure 2The experimental results showed that the bone marrow cells in the femurs of mice in the blank control group were densely and evenly arranged, while the bone marrow in the femurs of mice in the model control group showed numerous vacuoles. In the positive control group and the low-dose group of the Chinese medicine composition, the vacuoles in the femoral bone marrow cells were significantly reduced. In the medium- and high-dose groups of the Chinese medicine composition, the bone marrow cells in the femurs returned to a dense and even arrangement, indicating that the Chinese medicine composition can help alleviate bone marrow damage after chemotherapy.

[0055] On the day after the experiment, peripheral venous blood was obtained from the mice, and the red blood cells in the peripheral venous blood were counted using a hematology analyzer. Figure 3 As shown, the results showed that the red blood cell (RBC) count in the peripheral blood of mice in the model control group was significantly reduced compared with the blank control group, and the count in the high-dose group of the Chinese medicine composition was significantly increased compared with the model control group, indicating that the high dose of the Chinese medicine composition helps to improve the erythropenia caused by chemotherapy.

[0056] On the day after the experiment, peripheral venous blood was obtained from mice, and lymphocytes in the peripheral venous blood were counted using a hematology analyzer. Figure 4 The results showed that the lymphocyte count in the peripheral blood of mice in the model control group was significantly lower than that in the blank control group; the lymphocyte count in the peripheral blood of mice in the positive control group was significantly higher than that in the model control group; and the lymphocyte count in the blood of mice in the Chinese medicine composition test group was higher than that in the model control group, with the high-dose group achieving a significant difference, indicating that a high dose of the Chinese medicine composition can help improve chemotherapy-induced lymphocytopenia.

[0057] On the day of the experiment, peripheral venous blood was obtained from mice, and various types of lymphocytes in the peripheral blood were detected by flow cytometry: T cells, B cells, and NK cells. Figure 5 As shown. The results showed that the number of T lymphocytes in the peripheral blood of mice in the model control group was significantly reduced compared with the blank control group, and the proportion of T lymphocytes in the peripheral blood of mice in the positive control group and the Chinese medicine composition test group was significantly increased compared with the model control group. The number of B lymphocytes in the peripheral blood of mice in the model control group was significantly reduced compared with the blank control group, and the number of B lymphocytes in the peripheral blood of mice in the positive control group and the Chinese medicine composition test group was increased compared with the model control group, among which the increase level in the Chinese medicine composition test group reached statistical difference. The number of NK lymphocytes in the peripheral blood of mice in the model control group was significantly reduced compared with the blank control group, and the number of NK lymphocytes in the peripheral blood of mice in the positive control group and the Chinese medicine composition test group was increased compared with the model control group, among which the increase level in the Chinese medicine composition test group reached statistical difference.

[0058] In summary, the above results reveal that the Chinese medicine composition can alleviate the morphology of bone marrow damage after chemotherapy and promote the recovery of peripheral blood circulating immune cells, thereby showing the efficacy of alleviating bone marrow suppression after chemotherapy.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Use of a Chinese medicine composition in the preparation of a medicament for alleviating bone marrow suppression after chemotherapy, characterized in that: The Chinese medicine composition comprises, by weight, 18 to 30 parts of deer heart blood powder, 10 to 15 parts of ginseng, 4 to 10 parts of polygonatum, 4 to 10 parts of jujube seeds, 18 to 25 parts of coix seeds, 4 to 10 parts of citron, 8 to 15 parts of poria cocos and 8 to 15 parts of kudzu root.

2. The use according to claim 1, characterized in that The Chinese medicine composition comprises, by weight, 30 parts of deer heart blood powder, 12 parts of ginseng, 6 parts of polygonatum, 6 parts of jujube seeds, 20 parts of coix seeds, 6 parts of citron, 10 parts of poria cocos and 10 parts of kudzu root.

3. The use according to claim 1, characterized in that The traditional Chinese medicine composition improves bone marrow damage caused by chemotherapy.

4. The use according to claim 3, characterized in that The traditional Chinese medicine composition reduces bone marrow cell vacuolation.

5. The use according to claim 1, characterized in that The traditional Chinese medicine composition improves erythropenia caused by chemotherapy.

6. The use according to claim 1, characterized in that The traditional Chinese medicine composition improves lymphocytopenia caused by chemotherapy.

7. The use according to claim 6, characterized in that The lymphocytes include T cells, B cells and NK cells.

8. The use according to claim 1, characterized in that The preparation method of the Chinese medicine composition comprises the following steps: Ginseng, polygonatum, jujube seeds, coix seeds, citron, poria and kudzu root are mixed, the obtained mixture is decocted with water, and the decoction is concentrated to 1 / 3 of the original volume to obtain a medicinal material extract; the medicinal material extract is freeze-dried to obtain a medicinal material extract freeze-dried powder, which is then mixed with deer heart blood powder and sterilized to obtain the traditional Chinese medicine composition.

9. The use according to claim 1, characterized in that In the medicine, the content of the traditional Chinese medicine composition is 60 to 100 wt%.

10. The use according to claim 9, characterized in that The dosage forms of the drug include capsules, oral liquids, granules, tablets and pills.

Images