Traditional Chinese medicine compound microecological preparation for invigorating spleen to promote digestion and preparation process thereof

Abstract

The invention is applicable to the technical field of compound preparations, and provides a traditional Chinese medicine compound microecological preparation capable of invigorating spleen to promote digestion and a preparation process thereof. Atractylodes macrocephala koidz, immature bitter orange, elecampane, katsumadai seed, endothelium corneum gigeriae galli, hawthorn and pericarpium citri reticulatae are used as core components, lactic acid bacteria and saccharomycetes are combined to form a traditional Chinese medicine spleen and stomach regulating and micro-ecological absorption promoting synergistic system, the spleen and stomach digestion function can be improved, meanwhile, intestinal flora is directly regulated, and digestion and absorption are promoted; traditional Chinese medicine is extracted through two times of decoction, traditional Chinese medicine liquid is sterilized, and the traditional Chinese medicine liquid is subjected to segmented fermentation through lactic acid bacteria and saccharomycetes with precisely controlled viable count, so that synergistic conversion of effective components of traditional Chinese medicine and probiotics can be effectively promoted, absorption is more facilitated, and the traditional Chinese medicine is more suitable for long-term spleen and stomach function conditioning.

Description

Technical Field

[0001] The present invention belongs to the technical field of compound preparations, and in particular relates to a spleen-strengthening and digestion-promoting traditional Chinese medicine compound microecological preparation and a preparation process thereof. Background Art

[0002] Weak spleen and stomach, indigestion, and abdominal distension due to food accumulation are common digestive system problems, especially in children and adults with irregular diets. Modern medicine believes that these symptoms are closely related to factors such as insufficient gastrointestinal motility and an imbalance in the intestinal flora. First, weakened gastrointestinal motility leads to food retention; second, a decrease in beneficial intestinal bacteria and a proliferation of harmful bacteria cause an imbalance in the flora, affecting food decomposition and nutrient absorption.

[0003] Traditional Chinese medicine often uses Chinese herbs that strengthen the spleen and digestion (such as Atractylodes macrocephala, Hawthorn, etc.) to regulate spleen and stomach function and improve symptoms; while modern medicine uses the supplementation of specific probiotics (such as lactic acid bacteria) to directly regulate the balance of intestinal flora and promote food decomposition and nutrient absorption.

[0004] Research has found that supplementing with specific probiotics only treats the symptoms, not the root cause, and cannot achieve sustained improvement. Existing preparations are unable to synergize the two to address the problems of insufficient digestive function and malabsorption. Therefore, to address the above issues, a traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion and its preparation process are proposed. Summary of the Invention

[0005] The present invention provides a traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion and a preparation process thereof, aiming to solve the problems of insufficient digestive function and malabsorption.

[0006] The present invention is achieved by providing a traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, the preparation comprising traditional Chinese medicine components and microecological fermentation bacteria;

[0007] The Chinese medicinal components include the following raw materials in parts by weight: 15-18 parts of Atractylodes macrocephala, 10-12 parts of Citrus aurantium immaturus, 8-10 parts of Aucklandia lappa, 6-8 parts of Alpinia officinalis, 10-12 parts of Gallus gallus domestica, 10-12 parts of Hawthorn, and 10-12 parts of Tangerine peel;

[0008] The microecological fermentation bacteria include the following bacteria with the following viable bacteria ratio: lactic acid bacteria 2×10 8 -2.5×10 8 CFU / g, yeast 1×10 7 -1.3×10 7 CFU / g.

[0009] Preferably, the preparation further comprises excipients, wherein the excipients include sucrose powder and dextrin, and the mass ratio of sucrose powder to dextrin is 3:1-2:1.

[0010] Preferably, the mass ratio of the traditional Chinese medicine components, the microecological fermentation strains, and the excipients is 60:35:5-75:15:10.

[0011] Preferably, the raw materials of the Chinese medicinal components are all processed products, and Atractylodes macrocephala, Citrus aurantium immaturus, costus root, cardamom, chicken's gizzard lining, hawthorn, and tangerine peel are all stir-fried, and chicken's gizzard lining is processed by vinegar roasting.

[0012] A preparation process of a traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, comprising the following steps:

[0013] Mix Atractylodes macrocephala, Immature Citrus aurantium, Aucklandia lappa, Cardamomum villosum, Chicken's Gizzard Stone, Hawthorn, and Tangerine Peel according to weight, and grind into a fine powder of 40-80 mesh;

[0014] Add 4-5 times the amount of pure water by weight to the fine powder and soak for 30 minutes, then boil for 1-2 hours and filter to obtain a filtrate;

[0015] The filtrate is concentrated under reduced pressure to a relative density of 1.2-1.3 to obtain a Chinese medicine concentrate;

[0016] Lactic acid bacteria and yeast were inoculated into LB medium respectively and activated at 37°C and 200 rpm for 12 h.

[0017] The activated bacteria were transferred into the fermentation medium at an inoculum volume of 5% and cultured at a constant temperature of 37°C until the bacterial concentration was ≥1×10 8 CFU / ml, seed solution was obtained;

[0018] Add seed liquid to the traditional Chinese medicine concentrate and auxiliary materials according to the mass ratio, ferment at 30℃ for 8-12 hours, and stir every 4 hours;

[0019] When the total number of viable bacteria is ≥1×10 8 CFU / ml, heating at 60°C-70°C for 20-30 minutes to terminate the fermentation, and concentrating under reduced pressure to a water content of ≤20% to obtain a fermentation product;

[0020] The fermentation product is prepared into granules or powders by spray drying.

[0021] Preferably, the method further comprises: adding 3-4 times the weight of the medicinal residues with pure water, boiling for 1-1.5 hours, filtering and mixing the two filtrates.

[0022] Preferably, the fermentation medium comprises the following components: 10 g / L peptone, 5 g / L yeast extract, and 10 g / L glucose, and the pH of the fermentation medium is 6.5-7.0.

[0023] Preferably, the method further comprises: mixing the Chinese medicine concentrate with the auxiliary materials according to a mass ratio, sterilizing the mixture at 100° C. for 20 minutes, and then cooling the mixture to 30° C.

[0024] Preferably, the conditions for reduced pressure concentration are a pressure of -0.08 to -0.1 MPa and a temperature of 60-70°C.

[0025] Preferably, the granules or powders are low-temperature dried at a temperature of 45-50° C., and the moisture content of the granules or powders after drying is ≤5%.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] 1. The present invention uses Atractylodes macrocephala, Citrus aurantium immaturus, costus root, cardamom, chicken's gizzard lining, hawthorn, and dried tangerine peel as core components, combined with lactic acid bacteria and yeast, to form a synergistic system of traditional Chinese medicine regulating the spleen and stomach and promoting microecological absorption, which can improve the digestive function of the spleen and stomach, while directly regulating the intestinal flora and promoting digestion and absorption.

[0028] 2. The present invention extracts Chinese medicine through two decoctions, sterilizes the Chinese medicine liquid, and ferments the Chinese medicine liquid in stages by lactic acid bacteria and yeast with precisely controlled live bacteria counts. This can effectively promote the synergistic transformation of the effective ingredients of Chinese medicine and probiotics, is more conducive to absorption, and is more suitable for long-term spleen and stomach function conditioning. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be described in further detail. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0030] Control Example

[0031] A traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, comprising traditional Chinese medicine components and excipients;

[0032] The Chinese medicinal components include the following raw materials in parts by weight: 15 parts of Atractylodes macrocephala, 10 parts of Citrus aurantium immaturus, 8 parts of Aucklandia lappa, 6 parts of Alpinia officinalis, 10 parts of Gallus gallus domestica, 10 parts of Hawthorn, and 10 parts of Tangerine peel;

[0033] The auxiliary materials include sucrose powder and dextrin, and the mass ratio of sucrose powder to dextrin is 3:2.

[0034] Among them, the mass ratio of the traditional Chinese medicine components and excipients is 75:25.

[0035] The preparation process of the above-mentioned Chinese herbal compound microecological preparation for strengthening the spleen and promoting digestion comprises the following steps:

[0036] S1. Mix Atractylodes macrocephala, Immature Citrus aurantium, Aucklandia lappa, Cardamomum villosum, Chicken's Gizzard Stone, Hawthorn, and Tangerine Peel according to weight, and grind into a fine powder of 40-80 mesh;

[0037] S2. Add 10 times the amount of pure water by weight to the fine powder and soak for 30 minutes, boil for 1-2 hours and filter to obtain a filtrate;

[0038] S3. Add 8 times the amount of pure water to the medicinal residue according to the weight of the medicinal residue, boil for 1-1.5 hours, filter and mix the two filtrates.

[0039] S4, concentrating the filtrate under reduced pressure to a relative density of 1.2-1.3 to obtain a Chinese medicine concentrate;

[0040] The conditions for reduced pressure concentration are pressure -0.08 to -0.1 MPa and temperature 60-70°C;

[0041] S5. Mix the Chinese medicine concentrate with the excipients according to the mass ratio, sterilize at 100° C. for 20 min, cool to 30° C. and concentrate under reduced pressure to a water content of ≤20%;

[0042] The conditions for reduced pressure concentration are -0.08 MPa and 65°C;

[0043] S6. Prepare granules or powders by spray drying, and perform low-temperature drying on the granules or powders at a temperature of 45° C., wherein the moisture content of the granules or powders after drying is ≤5%.

[0044] Example 1

[0045] A traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, comprising traditional Chinese medicine components, microecological fermentation strains and excipients;

[0046] The Chinese medicinal components include the following raw materials in parts by weight: 15 parts of Atractylodes macrocephala, 10 parts of Citrus aurantium immaturus, 8 parts of Aucklandia lappa, 6 parts of Alpinia officinalis, 10 parts of Gallus gallus domestica, 10 parts of Hawthorn, and 10 parts of Tangerine peel;

[0047] Microecological fermentation bacteria include the following live bacteria: lactic acid bacteria 2×10 8 CFU / g, yeast 1×10 7 CFU / g;

[0048] The auxiliary materials include sucrose powder and dextrin, and the mass ratio of sucrose powder to dextrin is 3:2.

[0049] The mass ratio of the traditional Chinese medicine components, the microecological fermentation strains and the auxiliary materials is 60:35:5.

[0050] The raw materials of the Chinese medicinal components are all processed products. Atractylodes macrocephala, immature bitter orange, costus root, cardamom, chicken's gizzard lining, hawthorn, and tangerine peel are all fried, and chicken's gizzard lining is processed by vinegar roasting.

[0051] The preparation process of the above-mentioned Chinese herbal compound microecological preparation for strengthening the spleen and promoting digestion comprises the following steps:

[0052] S1. Preparation of Chinese medicine extract

[0053] Mix Atractylodes macrocephala, Immature Citrus aurantium, Aucklandia lappa, Cardamomum villosum, Chicken's Gizzard Stone, Hawthorn, and Tangerine Peel according to weight, and grind into 40-mesh fine powder;

[0054] Add 4 times the amount of pure water by weight to the fine powder and soak for 30 minutes, then boil for 1.5 hours and filter to obtain a filtrate;

[0055] Add 3 times the amount of pure water to the residue by weight, boil for 1.5 hours, filter and mix the two filtrates;

[0056] The filtrate was concentrated under reduced pressure to a relative density of 1.2 to obtain a Chinese medicine concentrate, wherein the conditions for the reduced pressure concentration were a pressure of -0.1 MPa and a temperature of 70°C;

[0057] S2. Preparation of probiotic fermentation broth

[0058] Lactic acid bacteria and yeast were inoculated into LB medium respectively and activated at 37°C and 200 rpm for 12 h.

[0059] The activated bacteria were transferred into the fermentation medium at an inoculum volume of 5% and cultured at a constant temperature of 37°C until the bacterial concentration was ≥1×10 8 CFU / ml, seed solution was obtained;

[0060] The fermentation medium contains 10 g / L peptone, 5 g / L yeast extract, and 10 g / L glucose, and the pH of the fermentation medium is 6.5.

[0061] S3. Compound fermentation

[0062] The Chinese medicine concentrate was mixed with the excipients according to the mass ratio, sterilized at 100°C for 20 minutes, and then cooled to 30°C;

[0063] Add seed liquid to the traditional Chinese medicine concentrate and auxiliary materials according to the mass ratio, ferment at 30℃ for 12 hours, and stir every 4 hours;

[0064] When the total number of viable bacteria is ≥1×10 8 CFU / ml, heating at 60°C for 20 min to terminate the fermentation, and concentrating under reduced pressure to a water content of ≤20% to obtain a fermentation product, wherein the conditions for the reduced pressure concentration are a pressure of -0.1 MPa and a temperature of 60°C;

[0065] S4. Prepare finished product

[0066] The fermentation product is prepared into granules or powder by spray drying, and the granules or powder are low-temperature dried at a temperature of 45° C., and the moisture content of the dried granules or powder is ≤5%.

[0067] Example 2

[0068] A traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, comprising traditional Chinese medicine components, microecological fermentation strains and excipients;

[0069] The Chinese medicinal components include the following raw materials in parts by weight: 18 parts of Atractylodes macrocephala, 12 parts of Citrus aurantium immaturus, 10 parts of costus root, 8 parts of cardamom, 12 parts of chicken's gizzard lining, 12 parts of hawthorn, and 12 parts of dried tangerine peel. The raw materials of the Chinese medicinal components are processed according to the preparation method in Example 1.

[0070] Microecological fermentation bacteria include the following live bacteria: lactic acid bacteria 2.5×10 8 CFU / g, yeast 1.3×10 7 CFU / g;

[0071] The auxiliary materials include sucrose powder and dextrin, and the mass ratio of sucrose powder to dextrin is 3:1.

[0072] The mass ratio of the traditional Chinese medicine components, microecological fermentation strains and auxiliary materials is 75:15:10.

[0073] The preparation process of the above-mentioned Chinese herbal compound microecological preparation for strengthening the spleen and promoting digestion comprises the following steps:

[0074] S1. Preparation of Chinese medicine extract

[0075] Mix Atractylodes macrocephala, Immature Citrus aurantium, Aucklandia lappa, Cardamomum villosum, Chicken's Gizzard Stone, Hawthorn Fruit, and Tangerine Peel according to weight, and grind into 80-mesh fine powder;

[0076] Add 5 times the amount of pure water by weight to the fine powder and soak for 30 minutes, then boil for 2 hours and filter to obtain a filtrate;

[0077] Add 4 times the weight of the medicinal residue to the pure water, boil for 1.5 hours, filter and mix the two filtrates;

[0078] The filtrate was concentrated under reduced pressure to a relative density of 1.3 to obtain a Chinese medicine concentrate, wherein the conditions for the reduced pressure concentration were a pressure of -0.08 MPa and a temperature of 70°C;

[0079] S2. Preparation of probiotic fermentation broth

[0080] Lactic acid bacteria and yeast were inoculated into LB medium respectively and activated at 37°C and 200 rpm for 12 h.

[0081] The activated bacteria were transferred into the fermentation medium at an inoculum volume of 5% and cultured at a constant temperature of 37°C until the bacterial concentration was ≥1×10 8 CFU / ml, seed solution was obtained;

[0082] The fermentation medium contains 10 g / L peptone, 5 g / L yeast extract, and 10 g / L glucose, and the pH of the fermentation medium is 7.0.

[0083] S3. Compound fermentation

[0084] The Chinese medicine concentrate was mixed with the excipients according to the mass ratio, sterilized at 100°C for 20 minutes, and then cooled to 30°C;

[0085] Add seed liquid to the traditional Chinese medicine concentrate and auxiliary materials according to the mass ratio, ferment at 30℃ for 8 hours, and stir every 4 hours;

[0086] When the total number of viable bacteria is ≥1×10 8 CFU / ml, heating at 70°C for 30 min to terminate the fermentation, and concentrating under reduced pressure to a water content of ≤20% to obtain a fermentation product, the conditions for the reduced pressure concentration being a pressure of -0.08 MPa and a temperature of 60°C;

[0087] S4. Prepare finished product

[0088] The fermentation product is prepared into granules or powder by spray drying, and the granules or powder are low-temperature dried at a temperature of 50° C., and the moisture content of the dried granules or powder is ≤5%.

[0089] Example 3

[0090] A traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, comprising traditional Chinese medicine components, microecological fermentation strains and excipients;

[0091] The Chinese medicinal components include the following raw materials in parts by weight: 16 parts of Atractylodes macrocephala, 12 parts of Immature Citrus aurantium, 9 parts of Aucklandia lappa, 7 parts of Cardamomum villosum, 11 parts of Gallus gallus domestica, 10 parts of Crataegus pinnatifida, and 10 parts of Tangerine peel. The raw materials of the Chinese medicinal components are processed according to the preparation method in Example 1.

[0092] Microecological fermentation bacteria include the following live bacteria: lactic acid bacteria 2×10 8 CFU / g, yeast 1×10 7 CFU / g;

[0093] The auxiliary materials include sucrose powder and dextrin, and the mass ratio of sucrose powder to dextrin is 2:1.

[0094] The mass ratio of the traditional Chinese medicine components, the microecological fermentation strains and the auxiliary materials is 60:35:5.

[0095] The preparation process of the above-mentioned traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion is carried out according to the steps of Example 2.

[0096] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A Chinese herbal compound microecological preparation for strengthening the spleen and promoting digestion, characterized in that: The preparation comprises traditional Chinese medicine components and microecological fermentation bacteria; The Chinese medicinal components include the following raw materials in parts by weight: 15-18 parts of Atractylodes macrocephala, 10-12 parts of Citrus aurantium immaturus, 8-10 parts of Aucklandia lappa, 6-8 parts of Alpinia officinalis, 10-12 parts of Gallus gallus domestica, 10-12 parts of Hawthorn, and 10-12 parts of Tangerine peel; The microecological fermentation bacteria include the following bacteria with the following viable bacteria ratio: lactic acid bacteria 2×10 8 -2.5×10 8 CFU / g, yeast 1×10 7 -1.3×10 7 CFU / g.

2. A spleen-strengthening and digestion-promoting Chinese medicinal compound microecological preparation according to claim 1, characterized in that: The preparation further includes auxiliary materials, which include sucrose powder and dextrin. The mass ratio of sucrose powder to dextrin is 3:1-2:

1.

3. A spleen-strengthening and digestion-promoting Chinese medicinal compound microecological preparation according to claim 2, characterized in that: The mass ratio of the traditional Chinese medicine components, the microecological fermentation strains and the auxiliary materials is 60:35:5-75:15:

10.

4. A spleen-strengthening and digestion-promoting Chinese herbal compound microecological preparation according to claim 1, characterized in that: The raw materials of the Chinese medicinal components are all processed products. Atractylodes macrocephala, immature bitter orange, costus root, cardamom, chicken's gizzard lining, hawthorn, and tangerine peel are all fried, and chicken's gizzard lining is processed by vinegar roasting.

5. A preparation process of a traditional Chinese medicine compound microecological preparation for strengthening the spleen and promoting digestion, characterized in that: The following steps are involved: S1. Mix Atractylodes macrocephala, Immature Citrus aurantium, Aucklandia lappa, Cardamomum villosum, Chicken's Gizzard Stone, Hawthorn, and Tangerine Peel according to weight, and grind into a fine powder of 40-80 mesh; S2. Add 4-5 times the amount of pure water by weight to the fine powder and soak for 30 minutes, boil for 1-2 hours and filter to obtain a filtrate; S3, concentrating the filtrate under reduced pressure to a relative density of 1.2-1.3 to obtain a Chinese medicine concentrate; S4. Lactic acid bacteria and yeast were inoculated into LB medium respectively and activated at 37°C and 200 rpm for 12 h. S5. Transfer the activated bacteria into the fermentation medium at an inoculum volume of 5% and culture at a constant temperature of 37°C until the bacterial concentration is ≥1×10 8 CFU / ml, seed solution was obtained; S7. Add the seed solution to the traditional Chinese medicine concentrate and auxiliary materials according to the mass ratio, ferment at 30°C for 8-12 hours, and stir every 4 hours; S8, when the total number of viable bacteria is ≥1×10 8 CFU / ml, heating at 60°C-70°C for 20-30 minutes to terminate the fermentation, and concentrating under reduced pressure to a water content of ≤20% to obtain a fermentation product; S9. The fermentation product is prepared into granules or powder by spray drying.

6. The preparation process of a spleen-strengthening and digestion-promoting traditional Chinese medicine compound microecological preparation according to claim 5, characterized in that: Also includes: According to the weight of the medicinal residue, add 3-4 times the amount of pure water to the medicinal residue, boil for 1-1.5 hours, filter and mix the two filtrates.

7. The preparation process of a spleen-strengthening and digestion-promoting traditional Chinese medicine compound proecological preparation according to claim 5, characterized in that: The fermentation medium comprises the following components: 10 g / L peptone, 5 g / L yeast extract, and 10 g / L glucose. The pH of the fermentation medium is 6.5-7.

0.

8. The preparation process of a spleen-strengthening and digestion-promoting traditional Chinese medicine compound microecological preparation according to claim 5, characterized in that: Also includes: The Chinese medicine concentrate and the auxiliary materials were mixed according to the mass ratio, sterilized at 100°C for 20 minutes, and then cooled to 30°C.

9. The preparation process of a spleen-strengthening and digestion-promoting traditional Chinese medicine compound proecological preparation according to claim 5, characterized in that: The conditions for reduced pressure concentration are pressure -0.08 to -0.1 MPa and temperature 60-70°C.

10. The preparation process of a spleen-strengthening and digestion-promoting traditional Chinese medicine compound proecological preparation according to claim 9, characterized in that: The granules or powders are low-temperature dried at a temperature of 45-50° C., and the moisture content of the dried granules or powders is ≤5%.