Production method and application of fulvic acid with content of 95%

Abstract

The invention discloses a production method and application of a fulvic acid culture medium with the content reaching 95%. The culture medium takes weathered coal as a raw material and comprises 30-70 g of humic acid, 4-30 g of fulvic acid, 2-20 g of ulmic acid and 2-20 g of humic acid. The ash content is 4-15%, the ratio of C to N is (25-30): 1, the organic matter is 40-80%, and the N.P.K content is 3-4%; the formula comprises 40-70% of humic acid, 6-30% of fulvic acid, 3-15% of ulmic acid and 3-15% of black humic acid. During production, 800-1000 g of raw materials and 400-500 g of sterile water are stirred, 1-2 g of stock seeds are added, the pH value is adjusted to be 6-7, the temperature of an incubator is controlled to be 48-60 DEG C, the humidity is controlled to be 75-85 DEG C, a proper amount of oxygen is added, and shading fermentation is conducted for 48-72 h. The obtained enzyme bacteria contain various organic acids, various amino acids, various oxygen-containing functional groups, various life elements and the like, the viability of beneficial bacteria reaches 90-260 billion / g and is far beyond the standard of 0.2 billion / g of the national standard, meanwhile, the bacteria can also be used for producing third-level bacterial manure which is suitable for field and gardening cultivation, and the effect is extremely good. After application, vicious circle of chemical fertilizer and pesticide can be converted into ecological virtuous circle, and technical progress is highlighted.

Description

Technical Field

[0001] The present invention relates to a production method and application of a composite microbial flora, and in particular to a composite flora culture medium formula capable of obtaining high-yield fulvic acid, and is particularly suitable for a production method and application of fulvic acid with a content of up to 95%. Background Art

[0002] Japanese scholars have isolated, purified, screened, identified and experimented with 24 types of beneficial bacteria from three types of microorganisms: bacteria in the soil (Becteria B), yeasts (Yest Y) in fungi, molds (filamentous fungi) (Mold M) and actinomycetes (Actinomyces A). They have then made a composite bacterial community in a certain proportion. This composite microbial community is enzyme bacteria.

[0003] There are two types of enzyme stock products: one is a food enzyme, trade name B.Y.M.-KOSD; the other is an agricultural enzyme, also known as agricultural enzyme, trade name B.Y.M.-FOOD. The concept of the present invention is precisely the production and application of the latter agricultural enzyme.

[0004] Enzymes possess strong aerobic fermentation capabilities. They can break down organic matter, pesticide residues, and fertilizers. They prevent soil salinization, dissolve solidified nutrients in the soil, increase soil permeability and water and fertilizer retention, kill soil pathogens, increase the content of beneficial bacteria, improve soil structure, and enhance crop quality and yield.

[0005] In December 1996, a Japanese company registered its microbial fertilizer with the Chinese Ministry of Agriculture, showing that its effective viable bacteria count was 65 million per gram, its enzyme activity was 639 units per gram (stock bacteria), and its enzyme-enhancing bacteria content was 104. 5 Ten thousand per gram, but it is expensive. Is it possible to find a culture medium that can increase the content of enzyme bacteria per gram while reducing the cost so that Chinese agriculture can afford it? The national standard for bio-fertilizers also requires the content of beneficial bacteria to be 20 million per gram. The present invention solves this technical problem through a new formula, opening a new page for high-yield, high-efficiency and practical enzyme bacteria. Summary of the Invention

[0006] The purpose of the present invention is to obtain a 95% fulvic acid content, and to find a better composite bacterial agent for the development of mineral-source humic acid bioengineering. On the basis of introduction, digestion and absorption, starting from the innovation of the fulvic acid culture medium, the new culture medium obtained can make the active bacteria per gram of beneficial bacteria reach 26 billion, far exceeding the national standard of 20 million / gram.

[0007] Another purpose of the present invention is to obtain an excellent and efficient production type of fulvic acid, expand the production of bacterial fertilizer, make the beneficial bacteria activity of 90-260 billion / gram, become the leader of fulvic acid fertilizer products, and after application, it can transform the vicious cycle of chemical fertilizers and pesticides into a benign ecological cycle, highlighting technological progress.

[0008] The present invention reforms the traditional culture medium for producing fulvic acid by using raw materials such as sugars and grain starch, and adopts an organic combination of multiple organic acids, multiple amino acids, multiple oxygen-containing functional groups, and multiple life elements, adding appropriate water, controlling temperature, humidity, pH value, and providing an appropriate amount of oxygen, and undergoing solid fermentation in an incubator, thereby achieving the special effect of high yield of fulvic acid and realizing the innovation of the present invention.

[0009] The object of the present invention is achieved as follows: a production method and application of a fulvic acid culture medium with a content of 95%, wherein the culture medium uses weathered coal as raw material, and its components are: weathered coal containing 30-70g of humic acid, 4-30g of fulvic acid, 2-20g of palmitic acid, and 2-20g of humic acid; 4-15% ash, 25-30:1 C / N ratio, 40-80% organic matter, and 3-4% NPK; the formula contains: 40-70% humic acid, 6-30% of fulvic acid, 3-15% of palmitic acid, and 3-15% of humic acid; during production, 800-1000 grams of raw materials are stirred with 400-500 grams of sterile water, 1-2g of original seed is added, the pH value is adjusted to 6-7, the incubator temperature is controlled at 48-60°C, the humidity is 75-85°C, oxygen is appropriate, and light-shielding fermentation is carried out for 48-72 hours.

[0010] The above step provides a method for producing 95% fulvic acid. The optimal culture medium uses weathered coal as raw material and comprises the following components: 65-68g humic acid, 5-8g fulvic acid, 3-15g palmitic acid, and 3-15g humic acid; 5-10% ash, 28-30:1 C / N ratio, 50-80% organic matter, and 3.5-4% NPK; the formula contains: 50-70% humic acid, 8-15% fulvic acid, 6-8% palmitic acid, and 6-8% humic acid; during production, 800-1000g of the raw material is stirred with 400-500g of sterile water, 1-2g of the original seed is added, the pH is adjusted to 7.0-7.2, the incubator temperature is controlled at 50-60°C, the humidity is 75-80°C, and oxygen is adequate, and the mixture is fermented in a dark environment for 50-70 hours.

[0011] The above method for producing 95% fulvic acid comprises an optimal culture medium using weathered coal as raw material, comprising: 68g humic acid, 8g fulvic acid, 4-10g palmitic acid, and 4-10g humic acid; 4-6% ash, a C / N ratio of 30:1, 60-80% organic matter, and 3-4% N, P, and K; the formula comprising: 60-70% humic acid, 8-9% fulvic acid, 6-8% palmitic acid, and 6-8% humic acid; during production, 800-1000g of the raw material is stirred with 400-500g of sterile water, 2g of the original seed is added, the pH is adjusted to 7.2, the incubator temperature is controlled at 50-60°C, the humidity at 75-80°C, and an appropriate amount of oxygen is provided, and the mixture is fermented in a darkened state for 50-65 hours.

[0012] In the production method of a fulvic acid with a content of 95%, the particle size of the refined raw material of the culture medium is 100-200 mesh and the moisture content is 8-11%.

[0013] The production method of a fulvic acid content of 95% in the above step is to mix the culture medium raw material and the original seed in a ratio of 100:2, place a layer of the original seed at intervals of 3-5 cm, put it into the fermentation box for solid fermentation, and fill the volume with materials to 2 / 3.

[0014] The obtained culture medium with a 95% fulvic acid content contains the following amino acids: aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, lysine, arginine, and tryptophan, which can make the activity of beneficial bacteria of fulvic acid 9 billion to 26 billion per gram.

[0015] The obtained culture medium with a fulvic acid content of 95% contains: total acid, carboxylic acid, phenolic carboxyl, total hydroxyl, alcoholic hydroxyl, total carboxyl, quinone, and non-carboxylic acid oxygen-containing functional groups, which can make the activity of beneficial bacteria of fulvic acid 90-260 billion / gram.

[0016] The obtained culture medium with a 95% fulvic acid content contains the following life elements: C, H, O, N, S, P, SI, AI, Fe, Ca, Mg, Na, K, Zn, Mn, Cu, and Ti, which can make the activity of beneficial bacteria of fulvic acid 9 billion to 26 billion per gram.

[0017] The present invention discloses a production and application of a fulvic acid with a content of 95%. The added original species and the raw material weathered coal contain a formula of humic acid, fulvic acid, palmitic acid and fulvic acid, which can also be used to produce tertiary bacteria strains of fulvic acid, and can also be directly used to produce fulvic acid organic fertilizers and organic and inorganic compound fertilizers and various special fertilizers.

[0018] The quality of so-called weathered coal is primarily evaluated by measuring its humic acid content. Humic acid, a complex, high-molecular-weight aromatic hydroxycarboxylic acid, is a hydrophilic, reversible colloid capable of both agglomeration and peptization, with a large internal surface area and strong adsorption capacity. Its molecular structure contains oxygen-containing reactive groups such as carboxyl and hydroxyl groups, as well as nitrogen rings such as pyridine, indole, and pyrrole, resulting in strong ion exchange and metal ion chelation capabilities. The presence of quinone and semiquinone groups in its structure also imparts a certain degree of redox activity. Therefore, humic acid products possess multiple functions, including ion exchange, chelation, surface adsorption, agglomeration, and peptization.

[0019] Fulvic acid contains more than 15 kinds of amino acids and more than 16 kinds of plant life elements. Fulvic acid and humic acid contain more than 8 kinds of plant life elements.

[0020] The product describes a method for producing 95% fulvic acid and the original bacteria used therein. The trade name is B·Y·M-FOOD, produced by Japan Pana Co., Ltd., and is used within the shelf life.

[0021] The technical parameters of the analysis report of the Central Laboratory of Xinjiang Academy of Agricultural Sciences support the results of the present invention.

[0022] Fulvic acid contains amino acids and life elements. Table 1.

[0023] Fulvic acid contains amino acids % Fulvic acid contains life elements % Aspartic acid 0.053 C 6.88 Threonine 0.026 H 2.41 Serine 0.031 N 0.18 glutamate 0.119 S 15.05 Glycine P 4.85 Alanine 0.059 SI 0.10 Cystine AI 1.45 Valine 0.045 Fe 0.91 Methionine 0.064 Ca 0.17 Isoleucine 0.025 Mg 0.98 Leucine 0.030 Na 5.95 Tyrosine 0.015 K 0.25 Lysine 0.028 Zn 0.02 Arginine 0.017 Mn 0.04 Tryptophan 0.007 Cu 0.01 Total g / 100g sample 0.52 Ti 0.01 The technical parameters in the analysis report (Table 2) of the Institute of Physical and Chemical Engineering of the Chinese Academy of Sciences support the results of the present invention.

[0024]

[0025] The above determination list fully demonstrates the scientific validity of the concept and technical route of the present invention. It is precisely because of the new formula and new process that the production bacteria contain a variety of amino acids, life elements, and oxygen-containing functional groups, achieving the inventive effect of obtaining a high yield of fulvic acid. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Symbols in the figure: 1 - Refined Raw Materials 2 - Quality Inspection 3 - Smash 4 - Sieve 5- Add water and stir 6- Add original species 7 - Fermentation 8-Drying packaging The accompanying figure of the specification is a process flow chart of the present invention, as shown in the figure: the culture medium ① raw material weathered coal 800 grams; ② qualified quality inspection; ③ crushing; ④ sieving, the particle size is 100-200 mesh, and the moisture content is 8-11%; ⑤ adding 400 grams of sterile water and stirring evenly; ⑥ adding 2.0g of original seed; pH value 6-7; the raw material and original seed are prepared in a ratio of 100:2, and a layer of original seed is placed at intervals of 3-5 cm, and then put into the fermentation box ⑦ solid fermentation, and the volume of the filling material is 2 / 3; controlling the incubator temperature at 50°C, humidity at 72-73°C, oxygen in an appropriate amount, and solid fermentation for 50-60 hours under light shielding; ⑧ drying, quality inspection and packaging. DETAILED DESCRIPTION

[0027] The present invention is further described below in conjunction with an embodiment. A method for producing 95% fulvic acid and its application, the embodiment is as follows: Example

[0028] Formula and process of humic acid bio-organic fertilizer: (Implementation standard: NY884-2004) Raw materials: ① Weathered coal, lignite, peat: 85-88g. Contains 30-70% humic acid, 4-30% fulvic acid, 2-30% humic acid and fulvic acid, with an organic matter content of 35% or more; pH 5.5-8.0; ② Bentonite: 10-15%; Contains SIO2, Al2O3, and Fe2O3; ③ Rare earth (mineral powder) and medical stone: 2-4%; Rare earth (mineral powder) and medical stone: 2-4%; Earth element Rex09, index 0.04-0.16%; ④ Water spray granulation, water dosage 15-20%, granulation is sufficient; ⑤ Fulvic acid grade 3 (solid or liquid), addition ratio 100:1-2; liquid inoculant is added with water according to the instructions and granulated using a water spray granulator (granulation moisture <30%; product moisture ≤10%); solid inoculant can be directly mixed evenly into the raw materials. ⑥ Bio-organic fertilizer particle size 3-5mm; ⑦ pH 5.5-8.

[0029] Continue with the previous step of production: Place the prepared culture medium into a sterilized fermentation box, fill it to 2 / 3 and add appropriate amount of oxygen; the fermentation box temperature is 60℃; fermentation time is 48-72 hours, shaded and fermented; pH value is 5-8 to prevent contamination by miscellaneous bacteria. Example

[0030] Formula and process for producing the third grade fulvic acid: 800-1000g of weathered coal, crushed and passed through a 100-mesh sieve, is mixed with 400-500g of sterile water and 2.0g of the original seed. The weathered coal contains 68g of humic acid, 8g of fulvic acid, 4-10g of palmitic acid, and 4-10g of fulvic acid; ash content is 4-10%; C / N ratio is 30:1; organic matter content is 60-80%; and NPK content is 3-4%. The raw material and original seed are placed in a ratio of 100:2, with the original seed layer spaced 3-5cm apart. The mixture is then placed in a solid fermentation box, with the material filling 2 / 3 of the volume. The pH value is 7.2; the incubator temperature is controlled at 48-60℃, the humidity is 75-85℃, the oxygen is appropriate, and the solid fermentation is carried out in a dark environment for 48-60 hours.

[0031] Example 3 Formula and process of making organic, inorganic and compound fertilizers with fulvic acid (BB fertilizer): Organic raw materials: 30-50% fulvic acid bio-fertilizer; 50-70% inorganic fertilizer mixed and prepared.

[0032] Inorganic raw materials: urea (46-0-0) 230-240 kg; diammonium phosphate (18-46-0) 375-385 kg; potassium chloride (0-0-60) 29-300 kg; the ratio of N, P20s, and KO is 19-19-19.

[0033] The formula for producing special fertilizers takes into account soil classification, planting varieties, per-acre yield requirements, soil testing and formula. This experiment was successfully applied to field cotton, grapes and horticultural cultivation.

Claims

1. A method for producing 95% fulvic acid, characterized in that: The culture medium uses weathered coal as raw material and comprises the following components: 30-70 g of humic acid, 4-30 g of fulvic acid, 2-20 g of palmitic acid, and 2-20 g of humic acid; 4-15% ash, 25-30:1 C / N ratio, 40-80% organic matter, and 3-4% NPK; the formula comprises: 40-70% humic acid, 6-30% fulvic acid, 3-15% palmitic acid, and 3-15% humic acid; during production, 800-1000 g of the raw material is stirred with 400-500 g of sterile water, 1-2 g of the original seed is added, the pH value is adjusted to 6-7, the incubator temperature is controlled at 48-60°C, the humidity is controlled at 75-85°C, an appropriate amount of oxygen is provided, and the mixture is fermented under shade for 48-72 hours.

2. The method for producing 95% fulvic acid according to claim 1, characterized in that: Youjia's culture medium is made from weathered coal and contains 65-68g of humic acid, 5-8g of fulvic acid, 3-15g of palmitic acid, and 3-15g of humic acid; 5-10% ash, a C / N ratio of 28-30:1, 50-80% organic matter, and 3.5-4% NPK. The formula contains 50-70% humic acid, 8-15% fulvic acid, 6-8% palmitic acid, and 6-8% humic acid. During production, 800-1000g of the raw material is stirred with 400-500g of sterile water, 1-2g of the original seed is added, the pH is adjusted to 7.0-7.2, and the incubator temperature is controlled at 50-60°C, the humidity at 75-80°C, and oxygen is maintained. Fermentation is performed in the dark for 50-70 hours.

3. The method for producing 95% fulvic acid according to claim 1, characterized in that: The optimal culture medium uses weathered coal as raw material, and its components include: 68g humic acid, 8g fulvic acid, 4-10g palmitic acid, and 4-10g humic acid; 4-6% ash, 30:1 C / N ratio, 60-80% organic matter, and 3-4% NPK; the formula contains: 60-70% humic acid, 8-9% fulvic acid, 7-8% palmitic acid, and 7-8% humic acid; during production, 800-1000g of the raw materials are stirred with 400-500g of sterile water, 2g of the original seed is added, the pH value is adjusted to 7.2, the incubator temperature is controlled at 50°C, the humidity is 80°C, the oxygen is appropriate, and the light is shielded and fermented for 50-65 hours.

4. The method for producing 95% fulvic acid according to claim 1, characterized in that: The particle size of the culture medium raw materials is 100-200 mesh and the moisture content is 8-11%.

5. The method for producing 95% fulvic acid according to claim 1, characterized in that: Mix the culture medium raw materials and the original seed at a ratio of 100:2, place a layer of the original seed at intervals of 3-5 cm, and put it into the fermentation box for solid fermentation, filling the volume with materials to 2 / 3.

6. A method for producing fulvic acid with a content of 95% according to claims 1, 4 and 5, characterized in that: The culture medium contains amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, lysine, arginine, and tryptophan, which can increase the activity of beneficial bacteria in the enzyme bacteria to 9-26 billion / gram.

7. The method for producing fulvic acid with a content of 95% according to claims 1, 4 and 5, characterized in that: The culture medium contains: total acid, carboxylic acid, phenolic carboxyl, total hydroxyl, 1 alcohol hydroxyl, total carboxyl, quinone, and non-carboxylic acid oxygen-containing functional groups, which can make the beneficial bacterial activity of enzyme bacteria 90-260 billion / gram.

8. The method for producing fulvic acid with a content of 95% according to claims 1, 4 and 5, characterized in that: The culture medium contains the following life elements: C, H, O, N, S, P, SI, AI, Fe, Ca, Mg, Na, K, Zn, Mn, Cu, and Ti, which can make the beneficial bacteria activity of enzyme bacteria 9-26 billion / gram.

9. The production and use of a 95% fulvic acid according to claims 1, 4 and 5, characterized in that: The added original species, the formula of weathered coal containing humic acid, fulvic acid, palmitic acid and black humic acid in the raw materials and the solid fermentation process can also be used to produce tertiary strains of enzyme bacteria, and can also be directly used to produce enzyme bacteria organic fertilizers and organic, inorganic compound fertilizers and various special fertilizers.

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