Liquorice seedling substrate and preparation method and application thereof

Abstract

The invention provides a liquorice seedling substrate and a preparation method and application thereof, and belongs to the technical field of crop cultivation. The invention provides a liquorice seedling raising substrate. The liquorice seedling raising substrate comprises turf, anaerobic fermentation rotten vegetable residues and platycladus orientalis fruit shell powder, the particle size of the platycladus orientalis shell powder is less than or equal to 0.45 mm; the preparation method of the platycladus orientalis fruit shell powder comprises the following steps: separating mature platycladus orientalis fruits to obtain shells; and drying and crushing the shells to obtain the platycladus orientalis shell powder. The liquorice seedling substrate can promote the growth of liquorice, improve the quality of the liquorice and the like. The result of the embodiment shows that the liquorice seedling culture substrate can improve the germination rate of liquorice, increase the plant height, stem diameter, root weight and root thickness of liquorice seedlings, and facilitate obtaining of strong liquorice seedlings.

Description

Technical Field

[0001] The invention belongs to the technical field of crop cultivation, and particularly relates to a liquorice seedling raising substrate, a preparation method and an application thereof. Background Art

[0002] Organic ecological soilless cultivation technology uses a substrate instead of natural soil. Instead of irrigating plant roots with traditional nutrient solutions, it uses organic solid fertilizers and direct water to irrigate crops. Organic substrates are made from organic materials such as crop straw, mushroom residue, peat, sawdust, and livestock and poultry manure, which are fermented or treated at high temperatures and then mixed in a specific proportion to form a relatively stable, fully nutritious cultivation substrate with a buffering effect.

[0003] At present, several commonly used seedling substrates at home and abroad include: rock wool, vermiculite, peat, slag ash, mushroom residue, perlite, fine sand, carbonized rice husk, sawdust, coconut shells, reed dust, shavings, corn cobs, rice bran, cottonseed hulls, tofu dregs and other organic, inorganic materials and microbial preparations. Among these matrix raw materials, after being compounded in a certain proportion, they can provide a good growth environment for seedlings.

[0004] Licorice is a perennial herb in the Leguminosae family. Its roots or rhizomes are used medicinally as a tonic in traditional Chinese medicine, boasting numerous benefits, including clearing heat and detoxifying, and relieving coughs and expectoration. Demand for licorice is currently increasing. Artificial cultivation of licorice typically involves direct seeding, a method that can result in low plant yields. Using soil as a substrate for licorice seedlings consumes a high percentage of the soil, and soil contamination by pesticides and heavy metals also severely impacts the yield and quality of licorice seedlings.

[0005] During the special project "Research on the Ecological Adaptability of Licorice in Saline-Alkali Land in Lanzhou New District," a large number of licorice seedlings were required to study the growth of the test licorice under different salt stress conditions indoors. However, during the cultivation of the licorice seedlings, it was discovered that simply applying existing soilless seedling cultivation methods often failed to produce robust licorice seedlings or seedlings. Therefore, providing a licorice seedling cultivation medium suitable for the growth of licorice seedlings is of great significance for the artificial cultivation of licorice. Summary of the Invention

[0006] In view of the defects in the prior art, the object of the present invention is to provide a licorice seedling growing matrix that can promote the growth of licorice seedlings and reduce the incidence of diseases and insect pests.

[0007] The purpose of the present invention is achieved through the following technical solutions: The present invention provides a licorice seedling growing matrix, which comprises, by volume, 8 to 10 parts of peat, 0.5 to 1 part of anaerobic fermentation of vegetable residue, and 1 to 4 parts of arborvitae cone shell powder; The particle size of the arborvitae cone shell powder is ≤0.45 mm; The preparation method of the oriental arborvitae cone shell powder comprises the following steps: separating shells from mature oriental arborvitae fruits; and drying and crushing the shells to obtain the oriental arborvitae cone shell powder.

[0008] Preferably, the licorice seedling growing matrix comprises 9-10 parts of peat, 0.7-1 parts of anaerobic fermentation of vegetable residues and 3-3.3 parts of Platycladus orientalis cone shell powder, calculated by volume.

[0009] Preferably, the licorice seedling growing matrix comprises 10 parts of peat, 1 part of anaerobic fermented vegetable residue and 3.3 parts of Platycladus orientalis cone shell powder, calculated by volume.

[0010] The present invention provides a method for preparing the licorice seedling culture medium described in the above technical solution, comprising: The peat, anaerobic fermentation of vegetable residue and Platycladus orientalis cone shell powder are mixed to obtain a licorice seedling medium.

[0011] The present invention provides the use of the licorice seedling substrate described in the above technical solution or the licorice seedling substrate prepared by the preparation method described in the above technical solution in any one or both of the following (1) and (2): (1) Licorice seedling cultivation; (2) Improve the quality of licorice seedlings.

[0012] Preferably, the licorice root includes any one or two or more of Glycyrrhiza inflata, Glycyrrhiza uralensis No. 1, Glycyrrhiza spinulosa and Glycyrrhiza glabra.

[0013] The present invention provides a method for raising licorice seedlings, comprising: The licorice seedling raising matrix described in the above technical solution or the licorice seedling raising matrix prepared by the preparation method described in the above technical solution is used as a cultivation matrix to cultivate licorice.

[0014] Preferably, the culture temperature is 7-30°C.

[0015] Preferably, during the cultivation, the licorice seeds are sown on the licorice seedling substrate and then covered with vermiculite.

[0016] Preferably, the thickness of the vermiculite covering is 1 to 2 cm.

[0017] Beneficial effects of the present invention: The invention provides a licorice seedling cultivation matrix, which comprises, by volume, 8-10 parts of peat, 0.5-1 part of anaerobic fermentation vegetable residue, and 1-4 parts of oriental arborvitae cone shell powder; the particle size of the oriental arborvitae cone shell powder is ≤0.45 mm; and the preparation method of the oriental arborvitae cone shell powder comprises the following steps: separating shells from mature oriental arborvitae fruits; and drying and crushing the shells to obtain the oriental arborvitae cone shell powder. The peat in the licorice seedling culture medium provided by the present invention is weakly acidic, light in weight, good in drainage and air permeability, strong in water retention capacity, and high in organic matter content, and can provide sufficient nutrients and a suitable growth environment for the growth of plants such as licorice, thereby being beneficial to promoting the growth of plants such as licorice; the anaerobic fermentation of vegetable residues is rich in organic matter and microorganisms, can improve soil structure, increase soil fertility, and promote plant growth; the arborvitae cone shell powder with a particle size of ≤0.45 mm is rich in various minerals and trace elements, can meet the growth requirements of plants such as licorice, has good water retention and air permeability, can improve soil structure, increase soil fertility, is beneficial to the growth and development of the root system, contains some natural antibacterial components, can inhibit the growth of pathogens and pests in the soil, reduce the occurrence of diseases and pests, improve the stress resistance of crops, and increase yield and quality. The licorice seedling medium provided by the present invention uses peat, anaerobic fermentation of vegetable residue and arborvitae cone shell powder as raw materials of the licorice seedling medium, which can synergistically promote the growth of licorice, significantly improve the germination rate, germination potential, plant height, stem thickness, root weight and root length of licorice, improve the quality of licorice, and improve the survival rate of seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 This is a commercial information diagram of peat used in the embodiments of the present invention; Figure 2 Collecting diagrams for the arborvitae cones; Figure 3 The figure shows the preparation process of Platycladus orientalis cone shell powder with a particle size of ≤0.45 mm; Figure 4 This is a diagram showing the specific preparation process of the licorice seedling medium; Figure 5 This is a diagram showing the preparation of the licorice seedling medium in the effect verification experiment of Application Example 1; Figure 6 This is an overall picture of the growth of licorice during the planting process of Application Example 1. DETAILED DESCRIPTION

[0020] The invention provides a licorice seedling cultivation matrix, which comprises, by volume, 8-10 parts of peat, 0.5-1 part of anaerobic fermentation vegetable residue, and 1-4 parts of oriental arborvitae cone shell powder; the particle size of the oriental arborvitae cone shell powder is ≤0.45 mm; and the preparation method of the oriental arborvitae cone shell powder comprises the following steps: separating shells from mature oriental arborvitae fruits; and drying and crushing the shells to obtain the oriental arborvitae cone shell powder.

[0021] As an optional embodiment of the present invention, the licorice seedling matrix includes 8 to 10 parts of peat by volume, or 8, 9 or 10 parts. As an optional embodiment of the present invention, when the peat is used as the raw material for the licorice seedling matrix, the particle size of the peat can be ≤2 mm, or 0.075 to 2 mm, or 0.45 to 1 mm. The present invention selects peat as the raw material for the licorice seedling matrix. Peat is weakly acidic, has good drainage and air permeability, strong water retention capacity, and high organic matter content. It can provide sufficient nutrition and suitable growth conditions for the growth of plants such as licorice, thereby helping to promote the growth of plants such as licorice.

[0022] As an optional embodiment of the present invention, the licorice seedling culture matrix includes 0.5 to 1 parts of anaerobic fermentation tail vegetable residues, or 0.5, 0.6, 0.7, 0.8, 0.9 or 1 parts, based on the volume of peat. The present invention has no special restrictions on the preparation method of the anaerobic fermentation tail vegetable residues, and the method of conventional anaerobic fermentation of tail vegetables in this field can be used. As an optional embodiment of the present invention, the preparation method of the anaerobic fermentation tail vegetable residues refers to the patent publication "A method for preparing organic fertilizer using vegetable tail vegetables, straw and animal excrement", and the patent publication number is the preparation method of anaerobic fermentation tail vegetable residues recorded in CN117567213A. The specific preparation method of the anaerobic fermentation tail vegetable residues is as follows: after collecting the tail vegetables, the tail vegetables are crushed to obtain crushed tail vegetables; the crushed tail vegetables are transferred to an anaerobic fermentation tank for anaerobic fermentation to obtain a fermentation product; the fermentation product is dried to obtain anaerobic fermentation tail vegetable residues. As an optional embodiment of the present invention, the tail vegetables include baby cabbage, lettuce, celery, broccoli, cabbage and Chinese cabbage tail vegetables; the mass ratio of the baby cabbage, lettuce, celery, broccoli, cabbage and Chinese cabbage tail vegetables can be 1:1:1:1:1:1:1. After obtaining the mixed tail vegetables, the present invention crushes the mixed tail vegetables. The present invention has no special restrictions on the method of crushing, and the conventional crushing method of the present invention can be adopted. As an optional embodiment of the present invention, the particle size of the crushed tail vegetables can be 3~5mm, or 3, 4 or 5mm. After obtaining the crushed tail vegetables, the crushed tail vegetables are transferred to an anaerobic fermentation tank, and anaerobic fermentation is carried out for 20 days under natural conditions to obtain a fermentation product. During the anaerobic fermentation process, the present invention mainly relies on the enzymes secreted by the hydrolytic bacteria carried in the tail vegetables to decompose the macromolecular organic matter such as cellulose, protein, fat, etc. in the tail vegetables into small molecular organic matter such as monosaccharides, amino acids, fatty acids, etc.

[0023] After obtaining the fermentation product, the present invention preferably performs solid-liquid separation on the fermentation product, and then dries the fermentation product, vegetable biogas residue, obtained by solid-liquid separation, to obtain anaerobic fermentation vegetable biogas residue. The present invention does not specifically limit the solid-liquid separation method, and any conventional solid-liquid separation method in the field can be adopted. After solid-liquid separation, the present invention takes the fermentation product, vegetable biogas residue and dries it. The present invention does not specifically limit the drying method, and any conventional drying method in the field can be adopted. As an optional embodiment of the present invention, the drying method can be sun-drying; the drying can be dried to a moisture content of ≤15%. After drying is completed, the present invention obtains anaerobic fermentation vegetable biogas residue. In the present invention, the anaerobic fermentation vegetable biogas residue is rich in organic matter and microorganisms, which can improve soil structure, increase soil fertility, and promote plant growth.

[0024] As an optional embodiment of the present invention, the licorice seedling medium includes 1 to 4 parts of Platycladus orientalis cone shell powder, or 3 to 3.3 parts, based on the volume of peat. In the present invention, the particle size of the Platycladus orientalis cone shell powder is ≤0.45 mm. As an optional embodiment of the present invention, the particle size of the Platycladus orientalis cone shell powder ranges from 0.075 to 0.45, and can be 0.1 to 0.45. In an embodiment of the present invention, the particle size of the Platycladus orientalis cone shell powder is ≤0.45 mm, wherein the mass ratio of Platycladus orientalis cone shell powder with a particle size <0.1 mm to Platycladus orientalis cone shell powder with a particle size of 0.1 to 0.45 mm is 7:4. In the present invention, the arborvitae cone shell powder has a high organic matter content, contains ingredients such as volatile oils, flavonoids and polysaccharides, has bactericidal and antiviral effects, can be used to prevent and control crop diseases and pests, protect crop health, contains substances that promote plant growth, such as cedarol, and can be used as a raw material for licorice seedling medium, which is beneficial to promoting licorice growth and improving the quality of licorice.

[0025] In the present invention, the method for preparing the arborvitae cone shell powder comprises: isolating the shells from mature arborvitae cones; and drying and crushing the shells to obtain the arborvitae cone shell powder. As an optional embodiment of the present invention, the method for preparing the arborvitae cone shell powder may include: collecting mature arborvitae cones to obtain arborvitae cones; after removing the arborvitae cone shells, drying, crushing, and sieving the shells to obtain the arborvitae cone shell powder. The present invention does not specifically limit the source of the arborvitae cones; any conventional arborvitae cones in the art may be used. The arborvitae cones of the present invention may be obtained from arborvitae trees planted on campus. The criteria for determining the maturity of arborvitae cones in the present invention may be: the arborvitae cone shells are slightly cracked or the color changes from green to brown. After collecting mature arborvitae cones, the present invention preferably removes the arborvitae cone shells. The present invention does not specifically limit the method for removing the arborvitae cone shells; any conventional method in the art may be used. As an optional embodiment of the present invention, the method for obtaining the arborvitae cone shells can be to air-dry the arborvitae cones, then beat them with a willow stick to thresh and remove the seeds, leaving the shells, thereby obtaining the arborvitae cone shells. After obtaining the arborvitae cone shells, the present invention preferably performs drying on the arborvitae cone shells. The present invention does not specifically limit the drying method, and conventional drying methods in the art can be used. As an optional embodiment of the present invention, the drying method can be shade drying; the shade drying can be performed to a moisture content of ≤15%, or to a moisture content of 7.5% to 15%, or to 15%, 14%, 13%, 12%, 11%, 10%, 9%, 8%, or 7.5%. After drying, the present invention preferably performs pulverization on the dried arborvitae cone shells. The present invention does not specifically limit the pulverization method, and conventional pulverization methods in the art can be used. After obtaining the pulverized arborvitae cone shells, the present invention performs sieving on the arborvitae cone shells. As an optional embodiment of the present invention, the sieve can have a pore size of 40 mesh. The present invention preferably obtains the arborvitae cone shell powder with a particle size of ≤0.45 mm by sieving. In an embodiment of the present invention, the arborvitae cone shell powder with a particle size of ≤0.45 mm is crushed to a rate of 92.3%.

[0026] The licorice seedling matrix provided by the present invention combines peat, anaerobic fermentation of vegetable residues and arborvitae cone shell powder with a particle size of ≤0.45mm. The three are used together as the raw materials of the licorice seedling matrix. The obtained licorice seedling matrix is ​​rich in various minerals and trace elements, which is beneficial to promoting plant growth; it has good water retention and air permeability, can improve soil structure, increase soil fertility, and is beneficial to the growth and development of the root system; it contains some natural antibacterial ingredients, which can inhibit the growth of pathogens and pests in the soil and reduce the occurrence of diseases and insect pests. The licorice seedling matrix provided by the present invention can promote the root development of licorice, improve the stress resistance of licorice, and increase the yield and quality of licorice. The results of the examples show that the licorice seedling matrix has the effect of promoting the growth of licorice seedlings, can increase the germination rate of licorice seeds, can increase the plant height and stem thickness of licorice seedlings, improve the quality of licorice seedlings, etc., and thus is conducive to cultivating strong licorice seedlings.

[0027] The present invention provides a method for preparing the licorice seedling medium described in the above technical solution, comprising: mixing peat, anaerobic fermentation of vegetable waste residue, and arborvitae cone shell powder to obtain the licorice seedling medium. The present invention does not particularly limit the mixing method, and any conventional mixing method in the art can be used. The preparation method of the licorice seedling medium provided by the present invention is simple and easy to perform, and is conducive to the widespread use of the licorice seedling medium.

[0028] The present invention provides the use of the licorice seedling matrix described in the above technical solution or the licorice seedling matrix prepared by the preparation method described in the above technical solution in any one or both of the following (1) and (2): (1) culturing licorice seedlings; (2) improving the quality of licorice seedlings. As an optional embodiment of the present invention, the licorice includes any one or more of Glycyrrhiza uralensis, Glycyrrhiza uralensis No. 1, Glycyrrhiza uralensis and Glycyrrhiza glabra. The licorice seedling matrix provided by the present invention can be used for culturing licorice seedlings, increasing the plant height and stem diameter, root weight and root diameter of licorice seedlings, and increasing the growth rate of licorice, thereby facilitating the improvement of the quality of licorice seedlings and providing a basis and basis for large-scale cultivation of licorice.

[0029] The present invention provides a method for raising licorice seedlings, comprising: using the licorice seedling matrix described in the above technical solution or the licorice seedling matrix prepared by the preparation method described in the above technical solution as a cultivation matrix, and cultivating licorice seeds. As an optional embodiment of the present invention, when cultivating, the licorice seeds are sown on top of the licorice seedling matrix, and then covered with vermiculite; the thickness of the covering can be 1 to 2 cm, or can be 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2.0 cm. The present invention adopts the licorice seedling matrix to cover the seeds with vermiculite during the planting of licorice, mainly because vermiculite has good water retention and air permeability, and after covering, a stable humidity environment can be provided for seed germination, while avoiding matrix compaction, which is conducive to seed breaking through the soil; vermiculite can also regulate the microenvironment, fix the seeds, etc., to prevent seed displacement during watering or moving, and ensure sowing uniformity. In the present invention, the thickness of the vermiculite covering can ensure that the seeds emerge and grow normally. Relative to the thickness of the vermiculite covering provided by the present invention, if it is too thick and greater than 2.0 cm, the seeds will germinate slowly; if it is too thin and less than 1.0 cm, the seeds will easily emerge with a cap or the radicle will be exposed outside the matrix, which is not conducive to seedling growth. As an optional embodiment of the present invention, the temperature of the culture can be 7~30 ° C, or 10~28 ° C, or 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 ° C. In the planting process of licorice in the embodiment of the present invention, licorice is planted under room temperature conditions, and the minimum temperature of the room temperature conditions in a day is ≥7 ° C, and the maximum temperature is ≤30 ° C.

[0030] In order to further illustrate the present invention, the technical solution provided by the present invention is described in detail below with reference to the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0031] The peat used in the following examples of the present invention was purchased from Shandong Dengjian Agricultural Technology Co., Ltd. The product name is: peat, a granular cultivation medium, an organic mineral formed by the accumulation of dead and rotten plants on the ground. The relevant product information of peat is shown in the figure. Figure 1 shown.

[0032] The licorice variety seeds in the following examples were all from the "Licorice Biological Breeding Research Institute, Jiuquan City, Gansu Province".

[0033] Example 1 A licorice seedling medium comprises, by volume, 10 parts of peat, 1 part of anaerobic fermented vegetable residue, and 3 parts of oriental arborvitae cone shell powder; wherein the oriental arborvitae cone shell powder has a particle size of 0.45 mm or less.

[0034] The method for preparing anaerobic fermentation waste residues comprises collecting waste vegetables from baby cabbage, lettuce, celery, broccoli, cabbage, and Chinese cabbage, and mixing the various waste vegetables in a mass ratio of 1:1:1:1:1:1:1 to obtain a mixed waste residue; crushing the mixed waste residue into 3-5 mm particles, transferring the mixed waste residue to an anaerobic fermentation tank, and using the hydrolytic bacteria carried by the mixed waste residue to undergo anaerobic fermentation for 20 days under natural conditions to obtain a fermentation product; and passing the fermentation product through a solid-liquid separation device for solid-liquid separation to obtain fermentation product waste residue sludge having a moisture content of 65%. After obtaining the fermentation product waste residue sludge with a moisture content of 65%, it is dried under natural conditions to a moisture content of ≤15%, thereby obtaining anaerobic fermentation waste residue.

[0035] The preparation method of Platycladus orientalis cone shell powder is as follows: collect Platycladus orientalis cones after they are mature. Figure 2 The standard for judging the maturity of Platycladus orientalis cones is: the shell of Platycladus orientalis cones is slightly cracked or the color changes from green to brown. After collecting the mature Platycladus orientalis cones, dry them in the shade and beat them with a willow stick, thresh and remove the seeds, leaving the shell to obtain the Platycladus orientalis cone shell. Dry the Platycladus orientalis cone shell in the shade to a moisture content of 7.5%, crush it with a grinder, pass it through a 40-mesh sieve, and take the sieve to obtain Platycladus orientalis cone shell powder with a particle size of ≤0.45mm. See the preparation process diagram of Platycladus orientalis cone shell powder with a particle size of ≤0.45mm for details. Figure 3 The particle size of the arborvitae cone shell powder is ≤0.45 mm, wherein the mass ratio of the arborvitae cone shell powder with a particle size of <0.1 mm to the arborvitae cone shell powder with a particle size of 0.1-0.45 mm is 7:4. The arborvitae cone shell obtained by the above-mentioned pulverization has a fine powder rate of ≤0.45 mm of 92.3%.

[0036] Before preparing the licorice seedling medium, the peat was crushed to obtain peat with a particle size of ≤2 mm. Among the peat used here, the peat <0.45 mm accounted for 67.3%, the peat of 0.45~0.5 mm accounted for 31.2%, the peat of 0.5~1 mm accounted for 1.0%, and the peat of 1~2 mm accounted for 0.5%.

[0037] The preparation method of licorice seedling medium is as follows: peat, anaerobic fermentation of vegetable residue and arborvitae cone shell powder are mixed according to volume ratio to obtain licorice seedling medium. The specific preparation process is shown in the figure. Figure 4 shown.

[0038] Example 2 A licorice seedling growing matrix comprises, by volume, 10 parts of peat, 1 part of anaerobic fermented vegetable residue and 3.3 parts of arborvitae cone shell powder.

[0039] The source of the licorice seedling substrate raw materials or the raw material preparation method and the preparation method of the licorice seedling substrate are the same as those in Example 1.

[0040] The test report of the licorice seedling culture medium prepared in Example 2 is shown in Table 1.

[0041] Table 1 Nutrient element test results of the licorice seedling medium prepared in Example 2

[0042] Comparative Example 1 A licorice seedling growing matrix comprises the following components by volume: 10 parts of peat and 1 part of anaerobic fermented vegetable residue.

[0043] The source of the licorice seedling substrate raw materials or the raw material preparation method and the preparation method of the licorice seedling substrate are the same as those in Example 1.

[0044] Comparative Example 2 A licorice seedling growing matrix comprises, by volume, 10 parts of peat, 1 part of anaerobic fermented vegetable residue, and 2.2 parts of arborvitae cone shell powder.

[0045] The preparation method of the Platycladus orientalis cone shell powder is the same as that of Example 2, except that after obtaining the Platycladus orientalis cone shell powder, the Platycladus orientalis cone shell powder with a particle size of 0.45-0.50 mm is used as the raw material of the licorice seedling medium.

[0046] The sources of other raw materials of the licorice seedling substrate or the raw material preparation method and the preparation method of the licorice seedling substrate are the same as those in Example 1.

[0047] Comparative Example 3 A licorice seedling growing matrix comprises, by volume, 2 parts of peat, 1 part of vermiculite and 1 part of perlite.

[0048] The preparation method of the licorice seedling medium is as follows: peat, vermiculite and perlite are mixed according to volume parts to obtain the licorice seedling medium.

[0049] Application Example 1 The licorice seedlings were cultured using the licorice seedling growing substrates of Example 2 and Comparative Examples 1 to 3.

[0050] The experiment was conducted in a semi-intelligent greenhouse on the campus of Lanzhou Modern Vocational College.

[0051] The experiment will start on September 30, 2024.

[0052] Experimental methods: Pretreatment of licorice seedling substrate: Use a fine-meshed watering can or sprayer to pre-wet the licorice seedling substrates of Example 2 and Comparative Examples 1 to 3 to the extent that they can be formed into a ball when held by hand and fall apart when dropped to the ground.

[0053] Filling licorice seedling matrix: Fill the licorice seedling matrix of Example 2 and Comparative Examples 1 to 3 into 50-hole hole trays respectively, and gently press the licorice seedling matrix with both hands to make each hole filled with licorice seedling matrix full and evenly filled, and then scrape off the excess matrix with a scraper. Figure 5 shown.

[0054] The sowing depth is 1~2cm. Place a hole tray of the same size directly above the hole tray filled with substrate. Make sure to align it up and down and left and right when drilling, otherwise the sowing hole will be off-center. If used to plant licorice seeds, the licorice seeds should be placed in the middle of the hole.

[0055] Sowing: Sow the Glycyrrhiza uralensis ( Glycyrrhiza inflate Bat.), Ganyu Licorice No. 1, Glycyrrhiza uralensis ( Glycyrrhiza pallidiflora Maxim.) and Glycyrrhiza glabra ( Glycyrrhiza glabra Four licorice seeds (L.) were planted in the different licorice seedling growing media described above, serving as experimental groups. Licorice seeds were manually sown in the center of each hole. Three replicates were performed per experiment, with nine holes per licorice variety.

[0056] Covering: Cover the licorice seeds with vermiculite. The thickness of the vermiculite is 1~2cm.

[0057] Watering: Put water into the sprayer and irrigate the substrate thoroughly so that water seeps out of the drainage holes at the bottom of each hole. Covering: Finally, cover the hole tray tightly with plastic film to keep warm and moisturize.

[0058] The growth of licorice during planting Figure 6 shown.

[0059] On November 11, 2024, the growth of licorice seedlings in each experimental group was tested. The test results of the licorice seedling height in each experimental group are shown in Table 2.

[0060] Table 2 Test results of licorice seedling height in each experimental group

[0061] As shown in Table 2, the licorice seedling matrix of Example 2 can more significantly promote the growth of the four licorice seedlings, increase the height of the licorice seedlings, and improve the growth quality of the licorice seedlings compared to the licorice seedling matrices of Comparative Examples 1 and 2. By comparing the results of Example 2 and Comparative Example 1, it can be concluded that adding arborvitae cone shell powder to the licorice seedling matrix can significantly increase the height of the licorice seedlings. By comparing the results of Example 2 and Comparative Example 2, it can be concluded that adding arborvitae cone shell powder with a particle size of ≤0.45mm to the licorice seedling matrix can significantly increase the height of the licorice seedlings, while adding arborvitae cone shell powder with a particle size of 0.45~0.5mm to the licorice seedling matrix not only cannot promote the growth of the licorice seedlings, but has an adverse effect on the growth of the licorice seedlings relative to Comparative Example 1. In summary, adding peat and anaerobic fermentation tail vegetable residue to the licorice seedling matrix while adding arborvitae cone shell powder with a particle size of ≤0.45mm is beneficial to promoting the growth of licorice seedlings.

[0062] During the growth of licorice, the growth of licorice in each experimental group was observed, and the observation results are shown in Table 3.

[0063] Table 3 Growth of licorice in each experimental group during its growth process

[0064] As shown in Table 3, the licorice seedling culture medium provided in Examples 1 and 2 of the present invention can not only promote the growth of licorice seedlings, but also significantly reduce the damage caused by diseases and insect pests to licorice seedlings and improve the quality of licorice seedlings.

[0065] On January 7, 2025, the root weight and root diameter of the licorice seedlings cultured in the licorice seedling substrate of Example 2 and Comparative Examples 1-2 were tested, and the results are shown in Tables 4-5.

[0066] Table 4 Root weight test results of licorice seedlings cultured in licorice seedling medium of Example 2 and Comparative Examples 1-2

[0067] Note: The vegetable residue in the table refers to the vegetable residue from anaerobic fermentation, the same below.

[0068] Table 5 Average root diameter test results of Glycyrrhiza uralensis seedlings cultured in Example 2 and Comparative Examples 1-2

[0069] As shown in Tables 4 and 5, the licorice seedling culture medium provided by the present invention can significantly increase the root weight of licorice seedlings compared with Comparative Example 1 and Comparative Example 2, and can also significantly increase the root diameter of licorice.

[0070] Application Examples 2 to 5 are experiments from the same batch, and the licorice, Knight No. 2 cabbage, and "Malachite Green" lettuce seeds used are from the same batch of seeds.

[0071] Application Example 2 Use licorice seedling medium to grow seedlings.

[0072] The experiment was conducted in a semi-intelligent greenhouse on the campus of Lanzhou Modern Vocational College.

[0073] The experiment will start on June 19, 2025.

[0074] The composition of the licorice seedling medium is as follows: 10 parts of peat, 1 part of anaerobic fermented vegetable residue and 3.3 parts of oriental arborvitae cone shell powder. The source of the licorice seedling medium raw materials and the preparation method of the raw materials and the preparation method of the licorice seedling medium are the same as those in Example 1.

[0075] Experimental methods: Pretreatment of licorice seedling medium: Use a fine-meshed watering can or sprayer to pre-wet the licorice seedling medium until it can be formed into a ball when held in the hand and will fall apart when dropped to the ground.

[0076] Filling the licorice seedling matrix: Fill the licorice seedling matrix into the hole tray respectively, press the licorice seedling matrix lightly with both hands to make the licorice seedling matrix filled in each hole full and evenly distributed, and then scrape off the excess matrix with a scraper.

[0077] Sow seeds at a depth of 1-2 cm. Place a plug tray of the same size directly above the one filled with substrate. Make sure to align the holes vertically and horizontally, otherwise the holes will be off-center. For licorice seeds, cabbage seeds, and lettuce seeds, place the seeds exactly in the center of the holes. The cabbage used is Knight No. 2, and the lettuce is "Malachite Green."

[0078] Sowing: Plant seeds of Ganyu Licorice No. 1 (Ganyu), Glycyrrhiza Glabra (Glycyrrhiza Glabra), Cavalier No. 2 Chinese Cabbage, and "Malachite Green" Lettuce in the aforementioned licorice seedling medium. Manually sow seeds in the center of each hole. Use a 12-hole tray, planting two seeds per hole.

[0079] Covering: Cover the licorice seeds with vermiculite. The thickness of the vermiculite is 1~2cm.

[0080] Watering: Put water into the sprayer and irrigate the substrate thoroughly so that water seeps out of the drainage holes at the bottom of each hole. Covering: Finally, cover the hole tray tightly with plastic film to keep warm and moisturize.

[0081] After the experiment started, the germination of seedlings in each experimental group was counted every day from June 19 to July 1. The germination rate and germination potential were counted on July 2. The results are shown in Table 6.

[0082] Table 6 Effects of licorice seedling medium on germination rate and germination potential

[0083] It can be seen from Table 6 that the licorice seedling medium can promote the growth of licorice seedlings to a certain extent and improve the germination rate and germination potential of licorice seedlings, but it is not conducive to the growth of Knight No. 2 cabbage and "Malachite Green" lettuce. Knight No. 2 cabbage and "Malachite Green" lettuce can hardly germinate in this seedling medium.

[0084] Application Example 3 Use licorice seedling medium to grow seedlings.

[0085] The experiment was conducted in a semi-intelligent greenhouse on the campus of Lanzhou Modern Vocational College.

[0086] The experiment will start on June 19, 2025.

[0087] Composition of the seedling medium: by volume, the licorice seedling medium comprises: 10 parts of peat and 3.3 parts of orientalis cone shell powder. The source of the licorice seedling medium raw materials and the preparation method of the raw materials and the preparation method of the licorice seedling medium are the same as those in Example 1.

[0088] The experimental method is the same as that of Application Example 2.

[0089] After the experiment started, the germination of seedlings in each experimental group was counted every day from June 19 to July 1. The germination rate and germination potential were counted on July 2. The results are shown in Table 7.

[0090] Table 7 Effects of licorice seedling medium on germination rate and germination potential

[0091] Application Example 4 Use seedling medium to grow seedlings.

[0092] The experiment was conducted in a semi-intelligent greenhouse on the campus of Lanzhou Modern Vocational College.

[0093] The experiment will start on June 19, 2025.

[0094] The composition of the seedling medium: peat. The source of the licorice seedling medium raw material is the same as that in Example 1.

[0095] The seeds of Knight No. 2 cabbage, "Malachite Green" lettuce, Glycyrrhiza uralensis (abbreviated as Zhangguo), Ganyu Glycyrrhiza uralensis No. 1 (abbreviated as Ganguo), Glycyrrhiza uralensis (abbreviated as Ciliaceus), Glycyrrhiza glabra (abbreviated as Guangguo) and Glycyrrhiza uralensis (abbreviated as Ural) were grown using peat seedling medium.

[0096] The experimental method is the same as that of Application Example 2.

[0097] After the experiment started, the germination of seedlings in each experimental group was counted every day from June 19 to July 1. The germination rate and germination potential were counted on July 2. The results are shown in Table 8.

[0098] Table 8 Effects of seedling medium on germination rate and germination potential

[0099] Application Example 5 Use seedling medium to grow seedlings.

[0100] The experiment was conducted in a semi-intelligent greenhouse on the campus of Lanzhou Modern Vocational College.

[0101] The experiment will start on June 19, 2025.

[0102] Composition of the licorice seedling matrix: The licorice seedling matrix is ​​composed of peat, vermiculite and perlite in a volume ratio of 2:1:1. The source of the licorice seedling matrix raw materials and the preparation method of the licorice seedling matrix are the same as those in Example 1.

[0103] The seeds of Glycyrrhiza uralensis (abbreviated as Zhangguo), Ganyu Glycyrrhiza uralensis No. 1 (abbreviated as Ganguo), Glycyrrhiza uralensis (abbreviated as Ciguo), Glycyrrhiza glabra (abbreviated as Guangguo) and Glycyrrhiza uralensis (abbreviated as Ural) were respectively grown using the above-mentioned seedling medium.

[0104] The experimental method is the same as that of Application Example 2.

[0105] After the experiment started, the germination of seedlings in each experimental group was counted every day from June 19 to July 1. The germination rate and germination potential were counted on July 2. The results are shown in Table 9.

[0106] Table 9 Effects of seedling medium on germination rate and germination potential

[0107] Tables 6-9 show that the licorice seedling medium provided by the present invention is not suitable for growing seedlings of Cavalier No. 2 Chinese cabbage and "Malachite Green" lettuce, and cannot germinate them. It is speculated that certain substances contained in the arborvitae cone shell powder are not conducive to seed germination and seedling cultivation of Cavalier No. 2 Chinese cabbage and "Malachite Green" lettuce. However, the licorice seedling medium provided by the present invention can significantly increase the germination rate and germination potential of different licorice varieties, promoting the growth of licorice seedlings.

[0108] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A licorice seedling medium, characterized in that By volume, it comprises 8-10 parts of peat, 0.5-1 part of anaerobic fermented vegetable residue and 1-4 parts of oriental arborvitae cone shell powder; The particle size of the arborvitae cone shell powder is ≤0.45 mm; The preparation method of the oriental arborvitae cone shell powder comprises the following steps: separating shells from mature oriental arborvitae fruits; and drying and crushing the shells to obtain the oriental arborvitae cone shell powder.

2. The licorice seedling raising matrix according to claim 1, wherein The licorice seedling culture medium comprises 9-10 parts of peat, 0.7-1 part of anaerobic fermentation of vegetable residue and 3-3.3 parts of arborvitae cone shell powder by volume.

3. The licorice seedling raising matrix according to claim 1 or 2, characterized in that The licorice seedling culture medium comprises 10 parts of peat, 1 part of anaerobic fermented vegetable residue and 3.3 parts of arborvitae cone shell powder by volume.

4. A method for preparing the licorice seedling culture medium according to any one of claims 1 to 3, characterized in that: include: The peat, anaerobic fermentation of vegetable residue and Platycladus orientalis cone shell powder are mixed to obtain a licorice seedling medium.

5. Use of the licorice seedling substrate according to any one of claims 1 to 3 or the licorice seedling substrate prepared by the preparation method according to claim 4 in any one or both of the following (1) and (2): (1) Licorice seedling cultivation; (2) Improve the quality of licorice seedlings.

6. The application according to claim 5, characterized in that The licorice includes any one or two or more of Glycyrrhiza uralensis, Glycyrrhiza uralensis No. 1, Glycyrrhiza uralensis and Glycyrrhiza glabra.

7. A method for raising licorice seedlings, characterized in that: include: The licorice seedling raising substrate according to any one of claims 1 to 3 or the licorice seedling raising substrate prepared by the preparation method according to claim 4 is used as a cultivation substrate to cultivate licorice.

8. The seedling raising method according to claim 7, wherein: The culture temperature is 7-30°C.

9. The seedling raising method according to claim 7, wherein: During cultivation, licorice seeds are sown on top of the licorice seedling substrate and then covered with vermiculite.

10. The seedling raising method according to claim 9, characterized in that: The thickness of the vermiculite covering is 1~2cm.

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