Sustainable clean water ecological perch culture method

Abstract

The invention relates to a sustainable clean water ecological weever culture method. The method comprises the following steps: S1, dry pond pretreatment; s2, steady-state environment adjustment: injecting clear water into the culture pond and putting a first microbial agent to reduce the pH value of the water body in the culture pond; then perennial submerged plants are planted, and steady-state environment adjustment is completed; wherein the planting density of the perennial submerged plants is not lower than 50 plants per square meter, and the planting area is not lower than 70% of the area of the aquaculture pond; s3, stocking weever fries and polyculture animals; s4, water quality regulation and disease prevention; s5, fishing and continuous breeding: fishing after breeding for a certain time; after fishing is completed, the steps S3-S4 are repeated, and continuous breeding is conducted. The technical problems that in the prior art, in the high-quality perch breeding process, the self-cleaning and stability maintaining capacity of a pond is poor, dependence on external intervention is high, the water quality is unstable, and the breeding cost is too high are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and in particular to a sustainable clear water ecological sea bass aquaculture method. Background Art

[0002] In recent years, with the increasing demand for a higher quality of life, the market competitiveness of high-quality commercial seabass has also increased. In the cultivation of high-quality seabass, water quality and stability are undoubtedly the most fundamental and influential factor. In existing technologies, seabass ponds often rely on frequent water changes and various external interventions, such as chemical agents, to maintain the basic water quality required for high-quality aquaculture, resulting in high aquaculture costs. Furthermore, while maintaining water quality within the aquaculture ponds, this approach also produces a large amount of aquaculture wastewater. To avoid environmental impact, this often requires expensive and energy-intensive factory-level equipment to filter and purify it before it can be safely discharged or recycled back into the ponds. This results in high costs for seabass aquaculture. (For example, while factory-scale recirculating water systems can produce high-quality seabass at high stocking densities, they require a variety of factory-level facilities and related interventions, resulting in extremely high costs and investment.) Furthermore, the use of chemical methods and other water purification methods can, to a certain extent, hinder the further improvement of seabass quality and the realization of its ecological benefits, leading to a decrease in its value. Although some technologies have attempted to introduce ecological elements to reduce breeding costs and further increase the value of sea bass, the effects are relatively poor. The self-purification and stability maintenance capabilities of the ponds are not ideal, and they are unable to effectively handle breeding metabolites (leftover bait, feces, etc.). It is difficult to maintain good water quality in a long-term and stable manner, especially in terms of water transparency and pH. It is even more difficult to achieve an ideal breeding state. Not only does the breeding process still require a lot of external intervention to maintain water quality, but it can only be bred at extremely low densities (generally around 300 fish per mu). Otherwise, the ecology is likely to collapse, which leads to a relatively high cost of ecological breeding. Summary of the Invention

[0003] (1) Technical issues to be solved

[0004] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a sustainable clear water ecological sea bass farming method, which solves the technical problems existing in the prior art in the process of high-quality sea bass farming, such as poor self-purification and stabilization ability of the pond, high dependence on external intervention, unstable water quality and excessively high farming costs.

[0005] (2) Technical solution

[0006] In order to achieve the above objectives, the main technical solutions adopted by the present invention include:

[0007] The present invention provides a sustainable clear water ecological seabass breeding method, comprising the following steps:

[0008] S1: Dry pond pretreatment: Add strong alkaline disinfectant to the dry pond to clean the pond;

[0009] S2: Steady-state environmental regulation: clean water is injected into the aquaculture pond and a first microbial agent is introduced to lower the pH of the water in the aquaculture pond; when the pH drops to a predetermined range, perennial submerged plants are planted in the aquaculture pond. When the water quality parameters in the aquaculture pond stabilize within the target range, steady-state environmental regulation is completed; the planting density of perennial submerged plants shall be no less than 50 plants per square meter, and the planting area shall be no less than 70% of the aquaculture pond area;

[0010] S3: Stocking sea bass fry and mixed culture animals: After the steady-state environment is adjusted, sea bass fry and mixed culture animals are stocked in the breeding pond, including shellfish and filter-feeding fish; the stocking density of sea bass fry is controlled at 500-1500 per mu; the amount of shellfish stocked does not exceed 200kg / mu; the amount of filter-feeding fish stocked does not exceed 60 per mu;

[0011] S4: Water quality control and disease prevention: Regularly add a second microbial agent to the aquaculture pond to inhibit the growth of algae and other bacteria;

[0012] S5: Harvesting and continuous breeding: Harvesting is carried out after a certain period of breeding; after the harvesting is completed, S3-S4 are repeated for continuous breeding.

[0013] Optionally, in S2, the dosage of the first microbial agent is 1-2 kg / mu; in parts by weight, the first microbial agent comprises 50-60 parts of lactic acid bacteria agent, 12-14 parts of yeast agent, 40-45 parts of Bacillus subtilis agent and 8-13 parts of Bacillus licheniformis agent.

[0014] Optionally, in S2, before the first microbial agent is put into the breeding pond, it needs to be expanded: lactic acid bacteria and yeast are expanded by sealed fermentation, the expansion time is 3-4 days, and the expansion multiple is 50-500 times; Bacillus subtilis and Bacillus licheniformis are expanded by open aeration, the expansion time is 10-12 hours, and the expansion multiple is 10-50 times.

[0015] Optionally, in S2, after the first microbial agent is added to the breeding pond, 500-1500 parts by weight of an expander are added to the breeding pond every 3-9 days until steady-state environmental adjustment is completed.

[0016] Optionally, in S2, when the pH of the water in the breeding pond drops to within the range of 7.0-8.0, perennial submerged plants are planted; the perennial submerged plants include Vallisneria, Water Chestnut and Foxtail Algae; the planting density of perennial submerged plants is 80-120 plants / square meter, and the planting area accounts for 80%-100% of the breeding pond area; among the planted perennial submerged plants, the number of Vallisneria accounts for 80%-90% of the total planting number, the number of Water Chestnut accounts for 10%-20%, and the number of Foxtail Algae accounts for 0%-10%.

[0017] Optionally, in S2, before planting perennial submerged plants, the water depth in the breeding pond shall not exceed 50 cm; after a certain period of planting, clean water shall be injected into the breeding pond again to keep the water depth within the range of 1.5-2.0 m; the water quality parameters are stabilized to the target range when: the pH of the water in the breeding pond is stable in the range of 7.0-8.5 in the morning and the transparency is ≥120 cm, the dissolved oxygen is ≥6 mg / L, and the chemical oxygen demand is ≤20 mg / L, and the steady-state environmental regulation is completed.

[0018] Optionally, in S3, the stocking density of sea bass fry is controlled at 500-1000 per mu; shellfish include ridge snails and river clams; the stocking density of ridge snails is 50-80 kg / mu, with a diameter of not less than 3 cm; the stocking density of river clams is 30-50 kg / mu, with a single weight between 0.5-0.75 catties; the weight of a single filter-feeding fish is in the range of 0.25-0.75 catties; filter-feeding fish include silver carp and bighead carp; the stocking density of silver carp is 10-20 tails / mu, and the stocking density of silver carp is 10-15 tails / mu.

[0019] Optionally, in S4, a second microbial agent is added once every 3-4 weeks, and the addition amount of the second microbial agent is 3-5 kg / mu; calculated by weight, the second microbial agent contains 40-70 parts of photosynthetic bacteria agent, 30-40 parts of nitrifying bacteria agent, and 10-20 parts of Bacillus subtilis agent.

[0020] Optionally, in S1, during the pretreatment of the dry pond, the bottom of the aquaculture pond is first deep-turned, and then quicklime is evenly sprinkled at a rate of 35-45 kg / mu; and then the pond is exposed to the sun for no less than 10 days.

[0021] Optionally, in S2, the planting time of perennial submerged plants is in early or mid-March in spring; in S3, the release of sea bass fry is in April-May or September-November; in S5, the breeding time of sea bass fry is 13-15 months; among them, when the sea bass fry are released in April-May, the length of the sea bass fry is between 5-7cm; when the sea bass fry are released in September-November, the length of the sea bass fry is 15-20cm.

[0022] (3) Beneficial effects

[0023] The beneficial effects of the present invention are as follows: Compared with the prior art, the sustainable clear water ecological sea bass breeding method of the present invention constructs a highly self-purifying closed ecological cycle through the combination of steady-state environmental regulation, appropriate stocking density and strict exogenous material input. It can decompose organic waste such as feces in the pond into nutrients through a stable microbial flora, and absorb nutrients such as nitrogen and phosphorus efficiently and quickly through high-density submerged plants. On this basis, filter-feeding fish and shellfish are used to accurately regulate the plankton community, further reducing the accumulation of pollutants such as nitrogen, phosphorus, and organic matter in the breeding pond, so that the water in the breeding pond can always be kept in a clear water state during the breeding process. The combined effect of the microbial flora and submerged plants can also effectively prevent eutrophication of the water body and inhibit the growth of miscellaneous bacteria and algae while maintaining the stability of clear water quality indicators, ensuring that the transparency of the water body in the breeding pond is stably maintained at more than 150 cm, achieving zero tailwater discharge during the breeding process, completely eliminating environmental pollution to the surrounding waters and significantly reducing breeding costs.

[0024] At the same time, the water quality of the clear water ecological sea bass breeding model of the present invention is stable and the breeding density is not high. In addition, due to the influence of the microbial flora, the sea bass of the present invention is basically free of disease during the breeding process, and the use of chemical preparations, hormones, antibiotics and other drugs in the breeding process can be avoided. While reducing the breeding cost, the safety of the obtained sea bass products is fundamentally guaranteed, and green, safe and non-toxic high-quality commercial sea bass is obtained. DETAILED DESCRIPTION

[0025] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below through specific implementation methods.

[0026] The present invention provides a sustainable clear water ecological seabass breeding method, comprising the following steps:

[0027] S1: Dry pond pretreatment: Add strong alkaline disinfectant to the dry pond of the aquaculture pond to clean the pond. The aquaculture pond is fully disinfected with strong alkaline disinfectant to kill pathogens, wild fish and other organisms that may destroy the ecological balance of the pond, and create an alkaline environment for the subsequent cultivation of bacterial flora.

[0028] S2: Steady-state environment regulation: clean water is injected into the aquaculture pond and a first microbial agent is added to lower the pH of the water in the aquaculture pond. Among them, the first microbial agent is mainly composed of acid-producing bacteria that can decompose organic matter and produce acid, supplemented by decomposing bacteria that can stably decompose organic matter in the aquaculture pond (such as dried perennial submerged plants, leftover bait, feces, dead algae and other organic matter). The organic acid produced by the acid-producing bacteria is used to neutralize the alkaline environment in the pond, and at the same time, it is used together with the decomposing bacteria to decompose the organic matter accumulated in the aquaculture pond. While improving the bottom quality and water pH of the aquaculture pond, the fertility of the soil is enhanced, the growth rate and survival rate of subsequent perennial submerged plants are accelerated, making them more lush and improving their ability to purify water quality. In addition, the microorganisms in the first microbial agent reproduce and iterate in the alkaline environment created by the clear pond, and can select a bacterial community with strong tolerance to alkaline environment in the aquaculture pond, which can normally produce acid or metabolize in an alkaline environment, thereby enhancing its ability to regulate the steady-state environment in the subsequent aquaculture pond. Of course, you can also choose strains that are resistant to alkaline environments from the beginning, but you still need to further select and breed them in an alkaline pond environment to make them more adaptable to the pond and form a stable bacterial community in the bottom mud. Moreover, when the bacteria in these first microbial agents form a stable bacterial community in the breeding pond, they can also inhibit the growth of miscellaneous bacteria to a great extent, especially acid-producing bacteria, whose acid-producing ability often gives them a stronger ability to inhibit the growth of miscellaneous bacteria. In addition, after a large area of ​​submerged plants is subsequently planted, their vigorous growth will also cause the water body to be too alkaline, especially in summer or at noon, the pH in the pond can even reach 10 or above, and the acid-producing bacteria can further avoid neutralizing the alkaline environment and prevent the pH in the breeding pond from becoming unstable.

[0029] When the pH drops to a predetermined range, it means that a relatively stable bacterial community has been formed in the breeding pond and the water quality will not have a significant impact on the planting, rooting and growth of perennial submerged plants. Perennial submerged plants can be planted in the breeding pond. The planting density of perennial submerged plants shall not be less than 50 plants per square meter, and the planting area shall not be less than 70% of the area of ​​the breeding pond. Among them, in order to ensure the subsequent purification ability of perennial submerged plants in the breeding pond, a larger area of ​​submerged plants needs to be planted. If the density of perennial submerged plants is too low, the water quality cannot be stably purified, resulting in a decrease in the ecological stability of the breeding pond, and eutrophication of the water body may occur. The water quality is prone to large fluctuations or can only remain stable at a lower breeding density, and the breeding cost may be high.

[0030] When the water quality in the breeding pond stabilizes to the target range, it means that the purification capacity of the perennial submerged plants is sufficient, and the bacterial community in the first microbial agent has cooperated with the perennial submerged plants to form a relatively stable environment in the breeding pond, completing steady-state environmental regulation.

[0031] S3: Stocking sea bass fry and mixed culture animals: After the steady-state environment is adjusted, sea bass fry and mixed culture animals are stocked in the breeding pond. The mixed culture animals include shellfish and filter-feeding fish. Among them, the stocking density of sea bass fry is controlled at 500-1000 per mu (it should be noted that compared with the stocking density of 3000-6000 sea bass in conventional earth ponds and the higher stocking density of cement ponds or factory farming, the present invention still belongs to low-density farming, and the sea bass has enough space for activities). The amount of shellfish released does not exceed 200kg / mu. The amount of filter-feeding fish released does not exceed 60 per mu. Under the above-mentioned breeding density and release amount, the breeding pond that has been adjusted to a steady-state environment can maintain good water quality for a long time without external intervention. At the same time, the breeding density of 500-1000 sea bass per mu can also effectively spread and reduce breeding costs. The addition of mixed culture animals can further spread costs while supplementing the ecological niche, so that the final breeding cost of the present invention is lower. Increasing the stocking density of sea bass or the number of mixed-breeding animals, while not necessarily leading to ecological collapse within the pond, could potentially reduce the pond's ecological stability and make it difficult to maintain high water quality over the long term. This could necessitate increased manual intervention for regulation, leading to higher aquaculture costs. If sea bass are stocked at too high a density, the amount of feed and feces produced will also be high, ultimately leading to elevated nitrogen and phosphorus levels or low dissolved oxygen in the water. These conditions require additional microbial inoculants, reduced feeding, or the installation of more aerators, leading to increased costs. While reducing the stocking density and the number of mixed-breeding animals can further improve pond environmental stability, the costs will be spread across fewer individuals, leading to a certain increase in aquaculture costs. During post-stocking and rearing, sea bass should be regularly fed antibiotic- and hormone-free feed at a daily rate of 2-3% of the average sea bass body weight.

[0032] S4: Water Quality Control and Disease Prevention: A second microbial agent is regularly added to the aquaculture pond to inhibit the growth of algae and other bacteria. Because the water quality in the aquaculture ponds of the present invention is good, the water is clear and pollution-free, and the primary bacterial flora is composed of bacteria from the first microbial agent, the aquaculture ponds are essentially disease-free, eliminating the need for hormones or chemicals, significantly reducing aquaculture inputs and improving the quality and value of the seabass. However, given the instability of the aquaculture process, and for safety and stability reasons, a certain amount of the second microbial agent needs to be regularly added to the aquaculture ponds to further inhibit the growth of bacteria and algae in the water, particularly cyanobacteria and green algae.

[0033] S5: Fishing and continuous breeding: Fishing is carried out after a certain period of breeding. After the fishing is completed, S3-S4 are repeated for continuous breeding. Among them, since the breeding method of the present invention can always maintain good water quality during the breeding process, and the submerged plants planted therein are perennial plants, after one breeding, the breeding pond does not need to be fully disinfected and the bottom is changed and can be directly used for the next breeding. There is no tail water discharge in the whole breeding process, and no tail water treatment is required. While being environmentally friendly, it can also further reduce the breeding cost to a certain extent.

[0034] The sustainable clean water ecological sea bass breeding method of the present invention constructs a highly self-purifying closed ecological cycle through steady-state environmental regulation, appropriate stocking density and strict exogenous material input. It can decompose organic waste such as feces in the pond into nutrients through microbial flora, and efficiently absorb nutrients such as nitrogen and phosphorus through submerged plants. On this basis, it uses filter-feeding fish and shellfish to accurately regulate the plankton community, further reducing the accumulation of pollutants such as nitrogen, phosphorus, and organic matter in the breeding pond, so that the water in the breeding pond can always be kept in a clear water state during the breeding process. The combined effect of microbial flora and submerged plants can also effectively prevent eutrophication of the water body and inhibit the growth of miscellaneous bacteria and algae while maintaining a stable clean water environment, ensuring that the transparency of the water body in the breeding pond is stably maintained at more than 150 cm, achieving zero tailwater discharge during the breeding process, completely eliminating environmental pollution to the surrounding waters and significantly reducing breeding costs.

[0035] At the same time, the water quality of the clear water ecological sea bass breeding model of the present invention is stable and the breeding density is not high. In addition, due to the influence of the microbial flora, the sea bass of the present invention is basically free of disease during the breeding process, and the use of chemical preparations, hormones, antibiotics and other drugs in the breeding process can be avoided. While reducing the breeding cost, the safety of the obtained sea bass products is fundamentally guaranteed, and green, safe and non-toxic high-quality sea bass with quality exceeding conventional food safety standards is obtained.

[0036] Moreover, the clean water (high dissolved oxygen, low density, and excellent water quality) ecological environment of the present invention can also significantly improve the freshness and nutritional value of sea bass meat. The sea bass cultured therein has strong mobility, firm muscle development, evenly distributed fat, and no earthy smell. It is a commercial sea bass with both excellent safety and top flavor quality.

[0037] Preferably, pond construction is required before S1. This includes the construction of a breeding pond and a water purification pool. The purification pool is equipped with several pumps connected to the breeding pond via pipelines to supply water when needed. The breeding pond is an earthen pond with a flat bottom, loam, and a silt thickness of 5-12 cm.

[0038] More preferably, the water purification pond is also an earthen pond with a water depth of 1.5-1.8 meters. Perennial submerged plants are also planted within the water purification pond, with the planting area covering 100%. Of these, Vallisneria serrata accounts for 90%-100% of the total planting area, Potamogeton crispus accounts for 0%-10%, and Myriophyllum serrata accounts for 0%-10%. The purification pond needs to be completed within four months before the completion of the aquaculture pond. After filling, the perennial submerged plants within it are used for purification. Microbial agents can also be used for regulation when necessary. Ultimately, the water in the purification pond must be crystal clear and the water quality indicators must be better than Class III water standards.

[0039] Preferably, in S1, during the dry pond pretreatment process: first, the bottom of the dry aquaculture pond is deep-plowed, and then 35-45 kg / mu of quicklime is evenly sprinkled thereon. After the sprinkling is completed, the pond bottom is exposed to sunlight for more than 10 days to ensure effective disinfection. After disinfection, clean water from the water purification tank can be introduced, and the first microbial agent method can be used to further control the environment. In addition to quicklime, a strong alkaline disinfectant such as sodium carbonate solution can also be used to provide an alkaline basic environment while ensuring effective disinfection.

[0040] Preferably, in S2, the addition amount of the first microbial agent is 1-6kg / mu, preferably 1-2kg / mu. By weight, the first microbial agent comprises 50-60 parts of lactic acid bacteria agent, 12-14 parts of yeast agent, 40-45 parts of bacillus subtilis agent and 8-13 parts of bacillus licheniformis agent. Wherein, lactic acid bacteria and yeast are all facultative anaerobic bacteria (some are also anaerobic bacteria), and bacillus subtilis and bacillus licheniformis are all strict aerobic bacteria. When the first microbial agent is put into aquaculture pond and breeds after a certain time, lactic acid bacteria and yeast are mainly enriched in sediment, and are bred in large quantities with sediment as culture medium, forming a stable flora that can exist for a long time, and bacillus subtilis and bacillus licheniformis are mainly enriched in the surface of water body and sediment. In the subsequent breeding process, Bacillus subtilis and Bacillus licheniformis can initially decompose the large molecular organic waste (leftover bait, feces, dead algae, etc.) deposited on the surface of water bodies and bottom mud (mainly deposited on the surface of bottom mud), and decompose them into smaller molecular organic nutrients. At this time, a large number of lactic acid bacteria and yeasts in the bottom mud can quickly decompose and consume the small molecular organic matter. The two are directly connected to avoid excessive distribution of small molecular nutrients in the water body, leading to eutrophication of the water body, and then causing harmful bacteria and algae to multiply in the water body.

[0041] At the same time, since a large amount of lactic acid bacteria is put into the present invention, the lactic acid bacteria can serve as the dominant bacterial community in the bottom mud and produce a large amount of acid in the subsequent reproduction process, which can not only effectively stabilize the pH value of the water body and avoid factors such as excessive alkalinity of the water body caused by excessive perennial submerged plants, but also the large amount of lactic acid produced by the anaerobic metabolism of the lactic acid bacteria can be combined with the metabolic product alcohol of the yeast to effectively inhibit the large-scale reproduction of other miscellaneous bacteria in the bottom mud and the water body, avoid the bottom mud with a large amount of organic nutrients precipitated from becoming a breeding ground for harmful bacteria, avoid the harmful bacteria from producing harmful substances through anaerobic fermentation, ensure the stability of water quality, and reduce diseases.

[0042] At the same time, it should be noted that, because the water in the breeding pond is highly alkaline before the first microbial agent is added to the breeding pond, the alkaline environment will neutralize the lactic acid secreted by the lactic acid bacteria in the first time, thereby causing their reproduction rate to be slower, while Bacillus subtilis and Bacillus licheniformis are more active in an alkaline environment. If the dosage of lactic acid bacteria is reduced or a higher proportion of Bacillus subtilis or Bacillus licheniformis is used, the reproduction of lactic acid bacteria may be inhibited by the alkaline environment and the large-scale reproduction of Bacillus subtilis and Bacillus licheniformis, and the lactic acid bacteria will not be able to reproduce in large quantities in the bottom mud and form a stable lactic acid bacteria community. The alkaline environment in the breeding pond cannot be changed by acid production, and the steady-state environment regulation cannot be played, thereby leading to the failure of steady-state environment regulation.

[0043] After the steady-state environmental adjustment is completed, these four bacterial communities can form a three-level cascade reaction of "decomposition-inhibition-transformation", realizing the rapid degradation of organic matter, inhibition of harmful bacteria and in-situ transformation of nutrients, significantly improving the redox state of the bottom mud, avoiding the accumulation of organic waste and secondary pollution after fermentation, and making the yellow-brown mud depth on the bottom mud surface reach more than 4 cm, the dry mud COD is less than 15 mg / kg, and the redox potential is greater than 0 mV, which can greatly increase the growth rate of perennial submerged plants.

[0044] Preferably, the first microbial agent is a lactic acid bacteria comprising at least one of Lactobacillus plantarum, Lactobacillus cocci, and Pediococcus acidilactici. Preferably, Lactobacillus plantarum or Lactobacillus cocci (need to undergo biosafety identification). More preferably, Lactobacillus plantarum is dispersed in the water body and can also preferably maintain the intestinal environment of seabass, reduce the risk of seabass suffering from bacterial diseases (such as enteritis, gill rot, etc.), increase the survival rate of aquaculture, improve the digestion and absorption of seabass, increase the growth rate of seabass, and reduce the feed conversion ratio.

[0045] Preferably, in S2, before the first microbial agent is put into the breeding pond, it is also necessary to expand the culture: wherein, lactic acid bacteria and yeast are expanded by sealed fermentation, the expansion time is 3-4 days and the expansion multiple is 50-500 times. Bacillus subtilis and Bacillus licheniformis are expanded by open aeration, the expansion time is 10-12 hours and the expansion multiple is 10-50 times. By weight, the expansion ratio of lactic acid bacteria is lactic acid bacteria agent: expansion agent: water = 1: 1-12: 35-45. The expansion ratio of yeast is yeast agent: expansion agent: water = 1: 8-12: 150-250. The expansion ratio of Bacillus subtilis and Bacillus licheniformis is Bacillus subtilis\Bacillus licheniformis agent: expansion agent: water = 1: 1-3: 15-25. Among them, because the water in the breeding pond is highly alkaline before the first microbial agent is put into the breeding pond, in order to avoid the problems such as the alkaline environment causing strong inhibition of lactic acid bacteria and yeasts and too slow reproduction rate, it is necessary to carry out expansion culture for a long time in advance, increase the total amount of these two bacteria species put into the breeding pond, and avoid the situation where their reproduction rate is slow in an alkaline environment, which leads to too long a time for steady-state environmental adjustment, and misses the time window for planting submerged plants or releasing sea bass fry. At the same time, a higher expansion multiple and the number after expansion can further enhance the ability of lactic acid bacteria to occupy a dominant position after entering the ecological pond, ensuring the stable regulation of the pond environment. In addition, expansion culture can also activate the bacteria in the corresponding bacterial agent, ensuring that they can quickly adapt to the environment and start activities after being put into the ecological pond.

[0046] For lactic acid bacteria, yeast, Bacillus subtilis, and Bacillus licheniformis, commercially available agents should be selected, as long as the number of viable bacteria in the agent meets the national standard. Of course, if conditions permit, microbial agents with higher standards can also be selected.

[0047] Preferably, the expansion agent is a natural expansion agent such as brown sugar, corn flour, malt extract, bran, or a commercial composite expansion agent, preferably brown sugar.

[0048] Preferably, in S2, after the first microbial agent is placed in the aquaculture pond, 500-1500 parts of an expander are added to the aquaculture pond every 3-9 days until the steady-state environment is regulated. By placing the expander in the aquaculture pond, sufficient nutrition is provided when the flora in the aquaculture pond is still unstable, ensuring that the lactic acid bacteria and yeast that have not yet been enriched in the mud can obtain sufficient nutrition when competing with Bacillus subtilis and Bacillus licheniformis, thereby accelerating the formation of a stable flora in the aquaculture pond. At the same time, sufficient nutrition can also accelerate the reproduction speed of the flora, ensure the acid production of the lactic acid bacteria, and accelerate the speed of steady-state environment regulation.

[0049] Preferably, in S2, before adding the first microbial agent, 100-200 g / mu of a chemical oxidizing oxygenator is introduced into the aquaculture pond to increase the oxygen concentration in the water and to disinfect the water in the aquaculture pond to a certain extent, thereby ensuring the subsequent expansion and regulation of the steady-state environment. The chemical oxidizing oxygenator includes potassium superphosphate, sodium percarbonate, or calcium peroxide, preferably potassium superphosphate.

[0050] Preferably, in S2, when the pH of the water in the breeding pond drops to 7.0-8.5, preferably within the range of 7.0-8.0, it indicates that a stable bacterial community has been formed in the breeding pond, lactic acid bacteria can produce a large amount of acid and adjust the pH, and perennial submerged plants can be planted.

[0051] Preferably, the perennial submerged plants include Vallisneria, Water Chestnut, and Myriophyllum. The planting density of perennial submerged plants is 80-120 plants per square meter, and the planting area accounts for 80%-100% of the aquaculture pond area. The number of Vallisneria accounts for 80%-90% of the total planting number, Water Chestnut accounts for 10%-20%, and Myriophyllum accounts for 0%-10%. Among them, by planting a variety of perennial submerged plants, different types of pollutants (or nutrients) can be better absorbed, and the water quality can be more comprehensively purified.

[0052] However, it should be noted that further increasing the planting density will better purify the water quality, but it may also cause the water in the breeding pond to be hypoxic at night or on cloudy days or the pH to be too high during the day, which will have a negative impact on sea bass farming.

[0053] More preferably, after the perennial submerged plants are planted, 500-2000g / mu·m water depth yeast peptide fertilizer and / or perennial submerged plant rooting powder is added to the water body to promote the rooting growth of the perennial submerged plants.

[0054] Preferably, in S2, before planting perennial submerged plants, the water injection depth does not exceed 50 cm to ensure that lactic acid bacteria and yeasts can effectively accumulate in the bottom mud and avoid hypoxia caused by excessive density of Bacillus subtilis and Bacillus licheniformis. After a certain period of planting, clean water is injected into the breeding pond again to maintain the water depth within the range of 1.2-2.0 m, preferably within the range of 1.5-2.0 m, and more preferably within the range of 1.5-1.8 m.

[0055] Preferably, steady-state environmental regulation is completed when the pH of the water in the aquaculture pond in the morning (around 6-10 am, preferably 7-8 am) is stable in the range of 6-9, preferably in the range of 7.0-8.5, and the transparency is ≥120 cm, the dissolved oxygen is ≥6 mg / L, and the chemical oxygen demand is ≤20 mg / L.

[0056] More preferably, the water level in the aquaculture pond is maintained within the range of 30-40 cm before and within one month after the planting of the perennial submerged plants in the aquaculture pond.

[0057] Preferably, in S2, the perennial submerged plants are planted by cuttings. The planting time of the perennial submerged plants is in early or mid-March in spring to ensure that the submerged plants have sufficient growth time and work together with the microbial flora to achieve steady-state environmental regulation.

[0058] More preferably, when water transparency ≥150cm, pH is 7.5-8.0, dissolved oxygen ≥6mg / L, ammonia nitrogen ≤0.1mg / L, total phosphorus ≤0.05mg / L, total nitrogen ≤1mg / L, nitrite ≤0.01mg / L, chemical oxygen demand ≤20mg / L, complete steady-state environment regulation, can carry out the breeding of seabass fry.In whole breeding process of the present invention, these numerical values ​​may produce fluctuation with the factors such as adding of sunshine or feed, but all can when not relying on external environment regulation, by the ecology in breeding pond, carry out rapid self-regulation, water quality is maintained within the above-mentioned scope, keep clear water ecology.Cultivating under clear water (high dissolved oxygen, low density, excellent water quality) ecological environment can significantly promote seabass fish meat deliciousness and nutritional value, improve the value of seabass.

[0059] The stocked sea bass fry should preferably be free of surface injuries, healthy and disease-free, of uniform size, and with a clear lateral line. Before entering the pond, the sea bass fry should be disinfected with 0.1-0.2ppm compound iodine for 10-15 minutes. After entering the pond, spray one to two times with vitamin C at a concentration of 150-250g / mu.

[0060] Preferably, in S3, filter-feeding fish of appropriate individual size should be selected for release to avoid being preyed upon by sea bass. At the same time, when releasing shellfish, especially herbivorous shellfish, the density of their release cannot be too high to avoid damaging submerged plants. Shellfish include ridge snails and river clams. The release density of ridge snails is 50-80kg / mu, and the diameter is not less than 3cm. The release density of river clams is 30-50kg / mu, and the weight of a single one is between 0.5-0.75 jin. The weight of a single filter-feeding fish is in the range of 0.25-0.75 jin. Filter-feeding fish include silver carp and bighead carp. The release density of silver carp is 10-20 tails / mu, and the release density of silver carp is 10-15 tails / mu. Filter-feeding fish and shellfish also need to be disinfected with 0.1-0.2ppm compound iodine for 10-15 minutes before entering the pond.

[0061] Preferably, S3 also includes daily breeding, feeding seabass commercial feed or ecological bait during seabass fry breeding, and feeding frequency is 2-3 times a day. It should be noted that substances such as hormones or chemicals should not be added to the feed to avoid affecting the quality of the seabass.

[0062] Preferably, in S3, if the planting area of ​​perennial submerged plants does not reach 100%, shellfish are released as much as possible in areas where perennial submerged plants are not planted.

[0063] Preferably, in S4, the sea bass of the present invention is basically free of disease during the breeding process, but if necessary, disease prevention work can also be carried out by using plant materials such as garlic and camphor leaves.

[0064] Specifically, garlic processing involves crushing fresh garlic cloves and adding clean water (or 5% alcohol) in a 1:2 ratio by weight. Soak for one day, stirring several times, and filtering to obtain a garlic extract. To use, spray the garlic extract evenly on or mix it into the feed. Allow the feed to fully absorb the extract before feeding and allow it to dry until the surface is no longer sticky. Garlic can enhance the antibacterial capacity of sea bass.

[0065] Camphor leaf preparation involves adding water to fresh camphor leaves, crushing them into a slurry, and then spreading the mixture over the pond at a rate of 15-25 kg per mu (approximately 1 acre) per meter of water depth. Camphor leaves are also used to enhance sea bass's resistance to parasites.

[0066] Preferably, S4 further includes perennial submerged plant management: when perennial submerged plants reach the water surface, they are harvested, each time harvesting half of their total length. From June to October, when perennial submerged plants are growing vigorously, the harvesting frequency can be appropriately increased based on the water pH measurement results to further ensure that the pH in the aquaculture pond does not become too alkaline due to excessive photosynthesis of submerged plants.

[0067] More preferably, each time perennial submerged plants are harvested, the remaining length must be ensured to be more than 30 cm to avoid the death of perennial submerged plants due to lack of nutrition. The harvesting frequency is increased by 1-2 times from June to October.

[0068] More preferably, in S4, the harvested aquatic plants can be used as ecological feed in other ecological farming, such as in the ecological farming of grass carp.

[0069] Preferably, in S4, a second microbial agent is added every 3-4 weeks or longer, and the dosage of the second microbial agent is 3-5 kg / mu. The second microbial agent is used to further regulate the water quality during the breeding process. Wherein, calculated by weight, the second microbial agent contains 40-70 parts of photosynthetic bacteria, 30-40 parts of nitrifying bacteria, and 10-20 parts of Bacillus subtilis. Wherein, since the amount of feed fed during the breeding process is generally large, it is not avoided that a large amount of feed is fed, resulting in a large amount of ammonia nitrogen that cannot be consumed in a short period of time. It is necessary to effectively reduce the concentration of ammonia nitrogen and nitrite in the breeding pond through the combined action of photosynthetic bacteria and nitrifying bacteria to avoid the large-scale growth of algae. Adding Bacillus subtilis increases the number of Bacillus subtilis in the water body of the breeding pond in a short period of time, thereby increasing the concentration of antibacterial substances secreted by Bacillus subtilis in the water body, further inhibiting the growth of miscellaneous bacteria. The input of the second microbial agent can further improve the transparency of the water body, and at the same time inhibit algae and miscellaneous bacteria, especially algae.

[0070] More preferably, the second microbial agent also needs to be expanded before being added, and the expansion multiple is 10-50 times.

[0071] Preferred, in S4, when finding the possibility that blue algae breaks out, for blue algae prevention, the 3rd microbial agent can be used to carry out, dosage is 3-5kg / mu, wherein calculated by weight, the 3rd microbial agent comprises lactic acid bacteria 60-70 part and bacillus subtilis 30-40 part, according to the identical expansion cultivation method adopted in the first microbial agent of fish, continuously sprinkles 3-4 time after expansion cultivation, can significantly suppress blue algae to occur.By the 3rd microbial agent temporary, significantly improve the lactic acid bacteria in the water body and the content of bacillus subtilis, seize the growth space of blue algae and make water body of short duration present the situation of skewness, suppress blue algae growth.If blue algae has broken out (generally only can occur in the case of neglecting management and control, under heavy rainfall, external water body flows into a large number of situations such as), equally also can be regulated and suppressed by the 3rd microbial agent, but its dosage should be 5-10 times when not breaking out.

[0072] Preferably, in S5, daily small-scale sea bass fishing can be carried out by angling. The impact of water flow can significantly increase the hook rate of sea bass, and the fishing efficiency is about 30-60 seconds per fish. During concentrated fishing, fishing can be carried out directly by drag net fishing or by using chemicals (such as tea saponin, a plant-based chemical, to avoid the use of chemical drugs and ensure that the clean water ecology is not polluted) in combination with drag net fishing.

[0073] More preferably, in S5, tea saponin is used as an auxiliary agent when fishing for sea bass. After spreading tea saponin, pulling the net 3-4 times can basically catch all sea bass, and the survival rate of captured sea bass can reach 95% or above.

[0074] Preferably, in S3, the seabass fry are released in April-May or September-November, preferably in April or September-October. When the seabass fry are released in April-May, the length of the seabass fry is between 5-7 cm. When the seabass fry are released in September-November, the length of the seabass fry is between 15-20 cm. When the seabass fry are first released into the aquaculture pond, seabass fry with a length of between 5-7 cm are preferably released in April-May. Seabass fry of appropriate size are released in the corresponding time window to ensure that the seabass fry can quickly adapt to the water temperature and clear water environment after release.

[0075] Preferably, in S5, the breeding time of sea bass fry is 13-15 months, and the release window of sea bass fry is adapted according to the breeding cycle of 13-15 months. Of course, if necessary, fishing can be advanced or delayed.

[0076] In order to better understand the above technical solutions, exemplary embodiments of the present invention will be described in more detail below. However, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a clearer and more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0077] Example 1

[0078] In 2024, clear water ecological sea bass farming was carried out at a clear water ecological farming base in Yixing.

[0079] In February 2024, the 20-acre sea bass breeding pond was renovated and constructed. First, the bottom of the pond was deep-turned, and 40 kg / acre of quicklime was evenly sprinkled, and the bottom of the pond was exposed to the sun for 10 days. Then the water was returned to 30 cm, and after 1 day, 150 g / acre of potassium persulfate was added, and the first microbial agent was added for steady-state environmental regulation. The dosage of the first microbial agent per acre was 1.5 kg, and it was added after expansion. Specifically, the first microbial agent contains 55 parts of fecal intestinal lactic acid bacteria agent (expanded 200 times), 12 parts of yeast agent (expanded 200 times), 40 parts of Bacillus subtilis agent (expanded 50 times), and 10 parts of Bacillus licheniformis (expanded 50 times).

[0080] In early March 2024, perennial submerged plants were planted throughout the pond at a rate of 120 plants per square meter. Vallisneria accounted for 85% of the total perennial submerged plants, Potamogeton crispus 10%, and Myriophyllum truncatum 5%. Yeast peptide fertilizer (0.8 kg / mu) was applied during this period to promote the rapid growth of perennial submerged plants.

[0081] By late March 2024, perennial submerged plants had grown throughout the pond, and the pond water quality indicators had also stabilized. The water quality was tested at 8 a.m. every day. During the last seven days of March, the pond water depth was measured continuously at 1.5m, the transparency was within the range of 1.4-1.5m, the pH value was within the range of 7.5-8.0, the dissolved oxygen was within the range of 8-10mg / L, ammonia nitrogen ≤0.1mg / L, total phosphorus ≤0.05mg / L, total nitrogen ≤0.6mg / L, nitrite ≤0.01mg / L, and chemical oxygen demand ≤10mg / L, completing steady-state environmental regulation.

[0082] On April 1, 2024, 20,000 seabass (average size 5-7cm), 200 silver carp (average weight 1.5kg), 200 bighead carp (average weight 1.5kg), 1,000kg of ring-finned snails, and 600kg of pleated clams (a type of river mussel) were released. Upon arrival, the seabass fry were heated and watered to ensure the water temperature in the water truck did not differ from the pond water temperature by more than 1°C. They were then disinfected with 0.2ppm compound iodine and transferred to aquaculture cages (previously set up in the aquaculture ponds) for a week before being released into the aquaculture ponds. Upon entering the cages, the seabass fry were sprayed with 0.2-0.3g / cubic meter of stress-stimulating vitamin C.

[0083] During the culture period, the sea bass were fed a factory-produced sea bass feed, once daily at 7:00 AM and once at 5:00 PM. Water quality was tested at 1:00 PM every 10-30 days and was stable, with a transparency of 1.4-1.5 μm, a pH of 8.5-9.5 (using pH test paper with an accuracy of 0.5), ammonia nitrogen ≤ 0.05 mg / L, nitrite ≤ 0.01 mg / L, dissolved oxygen ≤ 8-16 mg / L (stable within the range of 12-16 mg / L on sunny days), total phosphorus ≤ 0.05 mg / L, and chemical oxygen demand ≤ 10 mg / L.

[0084] As of June 12, 2025, a total of 17,326 seabass were harvested, with an average weight of 1.2 catties and a survival rate of 86.6%. Seabass are slender, golden in color, and have firm flesh without an earthy odor. They are high in protein, low in fat, rich in omega-3s, and free of antibiotics, hormones, and heavy metals.

[0085] Example 2

[0086] In 2024, clear water ecological sea bass farming was carried out at a clear water ecological farming base in Yixing.

[0087] In February 2024, a 5-mu sea bass breeding pond at the Yixing base was renovated and reconstructed. First, the pond bottom was deep-dredged, and 40kg / mu of quicklime was evenly sprinkled. The pond bottom was exposed to the sun for 10 days. The water was returned to 30cm, and 150g / mu of potassium persulfate was added. One day later, the first microbial agent was added at a dosage of 1.5kg per mu. After expansion, it was added. The first microbial agent contained 50 parts of Lactobacillus faecalis agent (expanded 200 times), 12 parts of yeast agent (expanded 200 times), 45 parts of Bacillus subtilis agent (expanded 50 times), and 13 parts of Bacillus licheniformis (expanded 50 times).

[0088] In early March 2024, perennial submerged plants were planted throughout the pond at a rate of 100 plants per square meter. Vallisneria accounted for 90% of the total perennial submerged plants, Potamogeton crispus 10%, and Myriophyllum sphaerocarpa 0%. Yeast peptide fertilizer (0.8 kg / mu) was applied during this period to promote the rapid growth of perennial submerged plants.

[0089] In late March 2024, after nearly a month of growth, perennial submerged plants basically covered the entire pond, and the pond water quality indicators also tended to stabilize. The water quality was tested at 8 am every day. During the last 7 days of March, the pond water depth was continuously measured at 1.5m, the transparency was in the range of 1.2-1.4m, the pH value was 7.5-8.5, the dissolved oxygen was 8-12mg / L, ammonia nitrogen ≤0.1mg / L, total phosphorus ≤0.05mg / L, total nitrogen ≤0.5mg / L, nitrite ≤0.01mg / L, chemical oxygen demand ≤10mg / L, completing the steady-state environmental regulation.

[0090] On April 1, 2024, 5,000 seabass (average size 5-7cm), 100 silver carp (average weight 1.5 jin), 50 bighead carp (average weight 1.5 jin), 250 kg of ring-finned snails, and 150 kg of pleated crown clams were released. Upon arrival, the seabass fry were heated and watered to ensure the water temperature in the water truck did not differ from the pond water temperature by more than 1°C. They were then disinfected with 0.2 ppm compound iodine and transferred to aquaculture cages for one week before being released into aquaculture ponds. Upon arrival, the seabass fry were sprayed with 0.2-0.3 g / m³ of stress-stimulating VC.

[0091] The breeding method is the same as that in Example 1, but the second microbial agent is put in once every 3 weeks during the breeding period, and the amount of the agent is 3kg / mu. Wherein, by weight, the second composite microbial agent comprises 60 parts of photosynthetic bacteria, 40 parts of nitrifying bacteria, and 10 parts of Bacillus subtilis, which are expanded 30 times before being put in. The water quality is tested once at 1 pm every 10-30 days during the breeding period. The water quality is stable, the transparency is within the range of 1.3-1.5m, the pH is within the range of 8.5-9.0, ammonia nitrogen ≤ 0.03mg / L, nitrite ≤ 0.01mg / L, dissolved oxygen is within the range of 9-17mg / L (stable within the range of 13-17mg / L on sunny days), total phosphorus ≤ 0.04mg / L, and chemical oxygen demand ≤ 10mg / L.

[0092] As of June 12, 2025, a total of 4,456 sea bass were caught, with an average size of 1.3 catties and a survival rate of 89.1%. The sea bass are slender in shape, golden in color, firm in texture and free of earthy smell. They are high in protein and low in fat, rich in Omega-3 and do not contain antibiotics, hormones or heavy metals.

[0093] Comparative Example 1

[0094] In 2024, sea bass farming was carried out at a clear water ecological breeding base in Yixing.

[0095] In February 2024, a 5-mu (approximately 1.5-1.5 acres) seabass pond was renovated. First, the pond bottom was deep-dredged and evenly sprinkled with quicklime at 30 kg / mu. The pond bottom was then exposed to sunlight for 10 days. Then, the water was backfilled to a depth of 30 cm and potassium persulfate (150 g / mu) was applied. One day later, a first microbial inoculant (1.5 kg / mu) was introduced. The inoculant consisted of 15 parts of Enterococcus faecalis, 15 parts of yeast, 50 parts of Bacillus subtilis, and 20 parts of Bacillus licheniformis. All inoculants were expanded 50-fold before introduction to maintain a steady-state environment.

[0096] In early March 2024, Vallisneria was planted at a density of 40 plants per square meter throughout the pond. During this period, yeast peptide fertilizer of 0.8 kg per mu was sprayed to promote the rapid growth of perennial submerged plants.

[0097] By late March 2024, the growth of Vallisneria had basically covered the entire pond but the density was low. The water quality was tested at 8 am every day. During the last 7 days of March, the pond water depth was measured continuously at 1.5m, the transparency was in the range of 0.7-1.0m, the pH value was in the range of 7.5-9.0, the dissolved oxygen was in the range of 6-8mg / L, the ammonia nitrogen was in the range of 0.25-0.3mg / L, the total phosphorus was in the range of 0.08-0.1mg / L, the total nitrogen was in the range of 0.8-1.0mg / L, the nitrite was in the range of 0.02-0.04mg / L, and the chemical oxygen demand was in the range of 18-24mg / L.

[0098] On April 1, 2024, 3,000 seabass (average size 5-7cm), 100 silver carp (average weight 1.5 jin), 50 bighead carp (average weight 1.5 jin), 250 kg of ring-finned snails, and 150 kg of pleated crown clams were released. Upon arrival, the seabass fry were heated and watered to ensure the water temperature in the water truck did not differ from the pond water temperature by more than 1°C. They were then disinfected with 0.2 ppm compound iodine and transferred to aquaculture cages for one week before being released into aquaculture ponds. Upon arrival, the seabass fry were sprayed with 0.2-0.3 g / m³ of stress-stimulating VC.

[0099] The breeding method is the same as that in Example 1. During the breeding period, the water quality is tested once every 10-30 days at 1 pm. The water quality is unstable, the transparency is in the range of 0.7-1.0m, the pH is in the range of 9.5-12.0, the ammonia nitrogen is in the range of 0.2-0.31mg / L, the nitrite is in the range of 0.03-0.08mg / L, the dissolved oxygen is in the range of 6-11mg / L (stable in the range of 8-11mg / L on sunny days), the total phosphorus is in the range of 0.08-0.17mg / L, and the chemical oxygen demand is in the range of 20-30mg / L.

[0100] As of June 12, 2025, a total of 2,134 sea bass were caught, with an average size of 1.1 catties and a survival rate of 71.1%.

[0101] As can be seen from the contents of the above embodiments, its aquaculture water body can stably reach a high transparent state for a long time, and the various indicators of water quality are all good, and it is possible to achieve a long period of time, self-purifying clear water ecological aquaculture, and ensure zero tail water discharge throughout the process, without the need for subsequent treatment, and with outstanding environmental advantages. The clear water aquaculture method of the present invention also effectively improves the survival rate of seabass aquaculture, significantly reduces the incidence of diseases, and significantly reduces the comprehensive aquaculture cost through the self-purification ability of the system and the substantial reduction in dependence on high-cost inputs (such as drugs, feed, water treatment), and can achieve efficient, green, and healthy aquaculture. Moreover, in terms of safety, the aquaculture method of the present invention relies on low-density aquaculture under clear water ecology, strictly eliminates the use of all banned drugs, hormones, and antibiotics, ensures from the source that there are no harmful substances remaining in the seabass body, and product safety meets and exceeds the green food standard, achieving safety and non-toxicity. Furthermore, thanks to the high dissolved oxygen, low density, and high-quality clean water environment, the seabass' vitality is enhanced, promoting firm muscle development and even fat distribution, effectively eliminating earthy odors, and significantly improving the fish's freshness, taste, and nutritional value. Ultimately, the resulting commercial seabass boasts both exceptional safety and premium flavor. While the seabass farming method in Comparative Example 1 also attempted to achieve clean water farming by constructing ecologically self-purifying ponds, the constructed ecological self-purification and stability capabilities were significantly poor, making it impossible to maintain good water quality over the long term. The water quality fluctuated significantly, resulting in a low seabass survival rate.

[0102] In summary, the present invention can solve the technical problems existing in the prior art in the process of high-quality sea bass breeding, such as poor self-purification and stabilization ability of the pond, high dependence on external intervention, unstable water quality and excessively high breeding costs.

[0103] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A sustainable clear water ecological sea bass farming method, characterized in that: The method comprises the following steps: S1: dry pond pretreatment: adding a strong alkaline disinfectant to the dry pond of the aquaculture pond to clean the pond; S2: Steady-state environmental regulation: clean water is injected into the aquaculture pond and a first microbial agent is introduced to lower the pH of the water in the aquaculture pond; when the pH drops to a predetermined range, perennial submerged plants are planted in the aquaculture pond. When the water quality parameters in the aquaculture pond stabilize within a target range, steady-state environmental regulation is completed; wherein the planting density of the perennial submerged plants is not less than 50 plants per square meter, and the planting area is not less than 70% of the area of ​​the aquaculture pond; S3: Stocking sea bass fry and mixed culture animals: After the steady-state environment is adjusted, sea bass fry and mixed culture animals are stocked in the breeding pond, and the mixed culture animals include shellfish and filter-feeding fish; the stocking density of the sea bass fry is controlled at 500-1500 per mu; the amount of shellfish stocked does not exceed 200 kg / mu; the amount of filter-feeding fish stocked does not exceed 60 per mu; S4: Water quality control and disease prevention: Regularly add a second microbial agent to the aquaculture pond to inhibit the growth of algae and other bacteria; S5: Harvesting and continuous breeding: Harvesting is carried out after a certain period of breeding; after the harvesting is completed, S3-S4 are repeated for continuous breeding.

2. The sustainable clean water ecological seabass breeding method according to claim 1, characterized in that: In S2, the addition amount of the first microbial agent is 1-2 kg / mu; in terms of weight, the first microbial agent comprises 50-60 parts of lactic acid bacteria agent, 12-14 parts of yeast agent, 40-45 parts of Bacillus subtilis agent and 8-13 parts of Bacillus licheniformis agent.

3. The sustainable clean water ecological seabass breeding method according to claim 2, characterized in that: In S2, before the first microbial agent is put into the breeding pond, it needs to be expanded: among them, lactic acid bacteria and yeast are expanded by sealed fermentation, the expansion time is 3-4 days, and the expansion multiple is 50-500 times; Bacillus subtilis and Bacillus licheniformis are expanded by open aeration, the expansion time is 10-12 hours, and the expansion multiple is 10-50 times.

4. The sustainable clear water ecological seabass breeding method according to claim 2, characterized in that: In S2, after the first microbial agent is added to the breeding pond, 500-1500 parts by weight of an expansion agent are added to the breeding pond every 3-9 days until the steady-state environment adjustment is completed.

5. The sustainable clean water ecological seabass breeding method according to claim 1, characterized in that: In S2, when the pH of the water in the breeding pond drops to within the range of 7.0-8.0, the perennial submerged plants are planted; the perennial submerged plants include Vallisneria, Water Chestnut and Foxtail Algae; the planting density of the perennial submerged plants is 80-120 plants / square meter, and the planting area accounts for 80%-100% of the breeding pond area; among the planted perennial submerged plants, the number of Vallisneria accounts for 80%-90% of the total planting number, the number of Water Chestnut accounts for 10%-20%, and the number of Foxtail Algae accounts for 0%-10%.

6. The sustainable clean water ecological seabass farming method according to claim 1, characterized in that: In S2, before planting the perennial submerged plants, the water depth in the breeding pond does not exceed 50 cm; after a certain period of planting, clean water is injected into the breeding pond again to keep the water depth within the range of 1.5-2.0 m; the water quality parameters are stabilized to the target range, which means that the steady-state environmental adjustment is completed when the pH of the water in the breeding pond is stable in the range of 7.0-8.5 in the morning and the transparency is ≥120 cm, the dissolved oxygen is ≥6 mg / L, and the chemical oxygen demand is ≤20 mg / L.

7. The sustainable clean water ecological seabass farming method according to claim 1, characterized in that: In S3, the stocking density of the sea bass fry is controlled at 500-1000 per mu; the shellfish include ridge snails and river clams; the stocking density of the ridge snails is 50-80 kg / mu, and the diameter is not less than 3 cm; the stocking density of the river clams is 30-50 kg / mu, and the weight of each clams is between 0.5-0.75 jin; the weight of each filter-feeding fish is within the range of 0.25-0.75 jin; the filter-feeding fish include silver carp and bighead carp; the stocking density of silver carp is 10-20 tails / mu, and the stocking density of silver carp is 10-15 tails / mu.

8. The sustainable clean water ecological seabass farming method according to claim 1, characterized in that: In S4, the second microbial agent is added once every 3-4 weeks, and the addition amount of the second microbial agent is 3-5 kg / mu; calculated by weight, the second microbial agent contains 40-70 parts of photosynthetic bacteria agent, 30-40 parts of nitrifying bacteria agent, and 10-20 parts of Bacillus subtilis agent.

9. The sustainable clean water ecological seabass farming method according to claim 1, characterized in that: In S1, during the pretreatment of the dry pond, the bottom of the aquaculture pond is first deeply turned over, and then quicklime is evenly sprinkled at a rate of 35-45 kg / mu; then the pond is exposed to the sun for no less than 10 days.

10. The sustainable clean water ecological seabass farming method according to claim 1, characterized in that: In S2, the planting time of the perennial submerged plants is in early or mid-March in spring; in S3, the sea bass fry are released in April-May or September-November; in S5, the breeding time of the sea bass fry is 13-15 months; among them, when the sea bass fry are released in April-May, the length of the sea bass fry is between 5-7 cm; when the sea bass fry are released in September-November, the length of the sea bass fry is 15-20 cm.