Dietary protein human supplement easy to absorb by human body and preparation method of dietary protein human supplement

Abstract

The invention belongs to the field of dietary food, and particularly discloses a dietary protein human supplement easy to absorb by human body and a preparation method thereof, the dietary protein human supplement comprises the following components by mass percentage: 30%-45% of hydrolyzed whey protein powder, 20%-25% of soybean oligopeptide, 15%-22% of bovine bone collagen peptide, and 8%-15% of composite active peptide; the composite active peptide comprises at least one first type of peptide for activating a small intestine oligopeptide transporter PEPT1 and at least one second type of peptide for inhibiting the activity of dipeptidyl peptidase-IV; according to the invention, a PEPT1 transporter is directly utilized for absorption through the small molecule peptide, the composite active peptide activates PEPT1 and inhibits DPP-IV at the same time, not only is peptide fragment transport promoted, but also degradation of the peptide fragment is reduced, and the synergistic effect of bovine bone collagen peptide high hydroxyproline and soybean peptide high branched chain amino acid is utilized, so that the muscle protein synthesis rate is increased and activated, and the protein absorption efficiency is greatly improved.

Description

Technical Field

[0001] The present invention belongs to the field of dietary food, and in particular relates to a dietary protein human supplement that is easily absorbed by the human body and a preparation method thereof. Background Art

[0002] Protein is a vital nutrient for the human body, forming the body's basic structure, participating in the synthesis of enzymes and hormones, and supporting the immune system and tissue repair. The daily protein requirement for adults varies, with a general recommendation of approximately 0.8 grams per kilogram of body weight. Athletes, pregnant women, or those with special health conditions may require higher protein intakes, often supplemented with dietary protein supplements.

[0003] Dietary protein supplements are products designed to supplement dietary protein, or to help those who need additional protein. They typically come in powders, tablets, liquids, or other forms, and are designed to help meet daily protein requirements, promote muscle repair, boost immunity, and support various bodily functions.

[0004] However, traditional protein supplements (such as protein powder, protein bars, etc.) generally have the problem of low absorption efficiency.

[0005] The core reasons include:

[0006] 1. Protein has a large molecular weight and needs to be enzymatically broken down into amino acids or small peptides in the digestive tract before it can be absorbed. This process is time-consuming and easily affected by the activity of digestive enzymes.

[0007] 2. The amino acid composition of a single protein source is unbalanced, and the absorption pathway is saturated, affecting absorption efficiency;

[0008] 3. Multiple peptide sources will lead to competitive inhibition of absorption, thereby reducing the absorption rate. Summary of the Invention

[0009] The object of the present invention is to provide a dietary protein human supplement that is easily absorbed by the human body and a preparation method thereof, so as to solve the problem of low absorption efficiency of traditional protein supplements mentioned in the above background technology.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] A dietary protein supplement for human body that is easily absorbed by human body, comprising, by mass percentage, 30% to 45% of hydrolyzed whey protein powder, 20% to 25% of soy oligopeptides, 15% to 22% of bovine collagen peptides, and 8% to 15% of composite active peptides;

[0012] Bovine bone collagen peptides include but are not limited to yak bone collagen peptides and cattle bone collagen peptides, preferably yak bone collagen peptides, which meet the requirements that the hydroxyproline content of bovine bone collagen peptides is ≥8% and the proportion of small molecule peptides with a molecular weight of <1000Da is ≥80%.

[0013] The composite active peptide comprises at least one first type peptide that activates the small intestinal oligopeptide transporter PEPT1 and at least one second type peptide that inhibits the activity of dipeptidyl peptidase-IV.

[0014] The composite active peptide satisfies a PEPT1 activation rate of ≥20% and a DPP-IV inhibition rate of ≥15%;

[0015] The first type of peptides are small molecule peptides containing Pro / Hyp or milk-derived peptides containing Gln, and the second type of peptides are C-terminal peptides containing Pro / Ile or hydrophobic peptides containing Trp;

[0016] The first type of peptide binds to the transmembrane domain of the PEPT1 transporter, causing its conformation to change and improving transport efficiency; the second type of peptide competitively binds to the serine active site of DPP-IV, inhibiting its enzymatic hydrolysis of the C-terminus of the peptide. The two peptides work together to extend the intestinal retention time of the peptide by more than 40%.

[0017] Preferably, the molecular weight of the hydrolyzed whey protein powder is ≤1000Da, the content of branched-chain amino acids in the soy oligopeptides is ≥18%, the hydroxyproline content of the bovine collagen peptide is ≥8%, and the proportion of components with a molecular weight <1000Da is ≥90%.

[0018] Preferably, the components are as follows by mass percentage: 45% hydrolyzed whey protein powder, 25% soy oligopeptide, 22% bovine collagen peptide, and 8% composite active peptide.

[0019] A method for preparing a dietary protein human supplement that is easily absorbed by the human body comprises:

[0020] S1. Pre-dry the soybean oligopeptide and bovine collagen peptide at 40°C and humidity ≤15% for 2 hours to reduce the water activity to below 0.3;

[0021] Eliminate static adsorption to avoid subsequent mixing agglomeration.

[0022] S2. Separately sieve the hydrolyzed whey protein powder, soy oligopeptide, bovine collagen peptide, and composite active peptide to control the particle size to 80-120 mesh;

[0023] S3, adding the sieved soybean oligopeptides and bovine collagen peptides into a mixing device and mixing them to obtain a first mixed material;

[0024] S4, adding the screened composite active peptide to the first mixed material and mixing them to obtain a second mixed material;

[0025] S5. Add the sieved hydrolyzed whey protein powder to the second mixed material and mix them to obtain a mixed product;

[0026] S6. After screening the mixed product, the mixed product is crushed by liquid nitrogen co-flow to obtain a dietary protein human supplement.

[0027] Preferably, the screened soybean oligopeptides and bovine collagen peptides are added to a mixing device for mixing at a temperature of 25-30° C., a humidity of ≤25%, a rotation speed of 150-200 r / min, a mixing time of 10-15 minutes, and a pH of 6.2-6.4.

[0028] Controlling the pH to 6.2-6.4 can avoid the Maillard reaction between the amino groups of soybean oligopeptides and the carboxyl groups of bovine collagen peptides, thereby preventing the loss of activity.

[0029] Preferably, the temperature for adding the sieved composite active peptide to the first mixed material for mixing is 25-30° C., the humidity is ≤25%, the rotation speed is 200-250 r / min, and the mixing time is 8-10 minutes.

[0030] Preferably, the temperature for adding the sieved hydrolyzed whey protein powder to the second mixed material for mixing is 20-25° C., the humidity is ≤30%, the rotation speed is 300-350 r / min, and the mixing time is 15-20 minutes.

[0031] Preferably, the liquid nitrogen accompanying flow pulverization temperature is -20°C, and the powder particle size after pulverization is controlled to be 15-50 μm.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The present invention scientifically compounds hydrolyzed whey protein powder, soy oligopeptides, bovine collagen peptides and composite active peptide polypeptide components. Small molecule peptides are directly absorbed by the PEPT1 transporter, and the composite active peptides simultaneously activate PEPT1 and inhibit DPP-IV, thereby promoting peptide transport and reducing its degradation. The synergistic effect of high hydroxyproline in bovine collagen peptides and high branched-chain amino acids in soy peptides is utilized to increase and activate the rate of muscle protein synthesis, thereby greatly improving protein absorption efficiency. DETAILED DESCRIPTION

[0034] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below.

[0035] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0036] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0037] Example 1: This example provides a dietary protein supplement that is easily absorbed by the human body. The components by mass percentage are: 45% hydrolyzed whey protein powder, 25% soy oligopeptide, 22% yak bone collagen peptide, and 8% composite active peptide;

[0038] The composite active peptide comprises at least one first type peptide that activates the small intestinal oligopeptide transporter PEPT1 and at least one second type peptide that inhibits the activity of dipeptidyl peptidase-IV;

[0039] The first type of peptides includes but is not limited to: small molecule peptides containing Pro / Hyp (such as LWH peptide in sea cucumber peptide) and milk-derived peptides containing Gln;

[0040] The second type of peptides (DPP-IV inhibitors) include but are not limited to: C-terminal peptides containing Pro / Ile (such as VPP in bovine spleen peptide) and hydrophobic peptides containing Trp.

[0041] The complex active peptide contains sea cucumber peptide 4% and bovine spleen peptide 4%.

[0042] Specifically, the molecular weight of hydrolyzed whey protein powder is 850Da, the content of branched-chain amino acids in soy oligopeptides is 21.3%, the content of hydroxyproline in bovine collagen peptides is 12.8%, and the components with a molecular weight <1000Da account for 95%.

[0043] The proportion of peptides with a molecular weight of ≤1000 Da was ≥85% in all peptide fractions.

[0044] Functional detection of composite active peptides:

[0045] PEPT1 activation rate: Caco-2 cell model to measure Gly-Pro transport rate;

[0046] Results: The effect was increased by 28.7% compared with the blank group (sea cucumber peptide activated PEPT1, and bovine spleen peptide provided substrate).

[0047] DPP-IV inhibition rate: fluorescent substrate Gly-Pro-AMC method;

[0048] Results: Enzyme activity was inhibited by 19.2% (bovine spleen peptide contains natural DPP-IV inhibitory peptide);

[0049] In vitro absorption rate: 94.2%.

[0050] From the above, we can see that based on the mechanism that dipeptides and tripeptides can be directly absorbed through the PEPT1 transporter of small intestinal epithelial cells, hydrolyzed whey protein provides fast energy peptides, which can be quickly absorbed, soy peptides activate amino acid transporters, and the Pro-Hyp-Gly tripeptide in bovine collagen peptides can be directly absorbed and utilized by the intestines. Targeted supplementation, the three types of peptides are absorbed by different pathways to avoid competitive inhibition and improve absorption efficiency; and the molecular weight of the peptide components ≤1000Da accounts for ≥95%, achieving easy absorption characteristics.

[0051] A method for preparing a dietary protein human supplement that is easily absorbed by the human body comprises:

[0052] S1. Pre-drying the soybean oligopeptide and bovine collagen peptide at 40°C and 15% humidity for 2 hours to reduce the water activity to 0.28;

[0053] S2. Separately sieve the hydrolyzed whey protein powder, soy oligopeptide, bovine collagen peptide, and composite active peptide to control the particle size to 100 mesh;

[0054] S3, adding the sieved soybean oligopeptides and bovine collagen peptides into a mixing device and mixing them to obtain a first mixed material;

[0055] S4, adding the screened composite active peptide to the first mixed material and mixing them to obtain a second mixed material;

[0056] S5. Add the sieved hydrolyzed whey protein powder to the second mixed material and mix them to obtain a mixed product;

[0057] S6. After screening the mixed product, the mixed product is crushed by liquid nitrogen co-flow to obtain a dietary protein human supplement.

[0058] Specifically, the sieved soybean oligopeptides and bovine collagen peptides were added to a mixing device for mixing at a temperature of 28° C., a humidity of 20%, a rotation speed of 180 r / min, a mixing time of 12 minutes, and a pH of 6.3.

[0059] Specifically, the sieved composite active peptide was added to the first mixed material and mixed at a temperature of 28° C., a humidity of 23%, a rotation speed of 220 r / min, and a mixing time of 9 minutes.

[0060] Specifically, the sieved hydrolyzed whey protein powder was added to the second mixed material and mixed at a temperature of 22° C., a humidity of 27%, a rotation speed of 320 r / min, and a mixing time of 18 minutes.

[0061] Specifically, the liquid nitrogen co-flow pulverization temperature is -20°C, and the powder particle size after pulverization is controlled at 35±5 μm.

[0062] Example 2: This example is basically the same as Example 1, except that the components by mass percentage are: 30% hydrolyzed whey protein powder, 20% soy oligopeptide, 15% bovine collagen peptide, and 10% composite active peptide;

[0063] The complex active peptide contains oyster peptide 6% and ginseng peptide 4%.

[0064] Specifically, the molecular weight of the hydrolyzed whey protein powder is 920 Da, the content of branched-chain amino acids in the soybean oligopeptide is 18.1%, and the content of hydroxyproline in the bovine collagen peptide is 9.5%.

[0065] Preparation method adjustment points:

[0066] Liquid nitrogen pulverization temperature was adjusted to -15°C (due to the weak heat sensitivity of ginseng peptide) → particle size 45±8μm;

[0067] The mixing humidity in the third stage is ≤35% (the proportion of whey protein is low and the hygroscopicity decreases).

[0068] Functional detection of composite active peptide (detection method is the same as Example 1):

[0069] PEPT1 activation rate: increased by 23.1% (oyster peptides are rich in zinc ion co-activator transporters);

[0070] DPP-IV inhibition rate: 22.4% inhibition (ginsenoside metabolite inhibits DPP-IV).

[0071] Example 3: This example is basically the same as Example 1, except that the components by mass percentage are: 38% hydrolyzed whey protein powder, 23% soy oligopeptide, 20% bovine collagen peptide, and 9% composite active peptide;

[0072] The complex active peptide contains wheat germ peptide 5%.

[0073] Specifically, the molecular weight of the hydrolyzed whey protein powder is 920 Da, the content of branched-chain amino acids in the soybean oligopeptide is 18.1%, and the content of hydroxyproline in the bovine collagen peptide is 9.5%.

[0074] Wheat germ peptide functional detection (detection method is the same as Example 1):

[0075] PEPT1 activation rate: increased by 26.5% (containing high-abundance glutamine dipeptide);

[0076] DPP-IV inhibition rate: 17.8% (the C-terminus of the peptide chain contains Pro-Ile structure).

[0077] Comparative Example 1: The components by mass percentage are: 50% hydrolyzed whey protein powder, 30% soy oligopeptide, and 20% bovine collagen peptide;

[0078] The hydroxyproline content of bovine collagen peptide is 6.2%.

[0079] Test results:

[0080] PEPT1 activation rate: +9.3% (whey protein alone);

[0081] DPP-IV inhibition rate: -2.1% (no inhibitory effect);

[0082] In vitro absorption rate: 71.5%.

[0083] Comparative Example 2: The composition ratio is the same as that of Example 1, except that:

[0084] All raw materials were mixed at once, with a mixing temperature of 30° C., a rotation speed of 250 r / min, and a mixing time of 30 min. Liquid nitrogen pulverization was not performed, and the ordinary pulverized particle size was 120 μm.

[0085] Test results: the agglomeration rate was 12% (due to electrostatic adsorption), the immune activity of bovine spleen peptide decreased by 38%, and the hydrolyzed whey protein powder absorbed moisture and cross-linked, resulting in a decrease in the bioavailability of the composite active peptides, causing the composite active peptides to be not fully utilized, and the in vitro absorption rate dropped to 79.6%.

[0086] It will be appreciated that in the development of any actual embodiment, as in any engineering or design project, numerous implementation-specific decisions may be made. Such a development effort may be complex and time-consuming, but will, for those of ordinary skill having the benefit of this disclosure, be a routine undertaking of design, fabrication, and production without undue experimentation.

[0087] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A dietary protein human supplement that is easily absorbed by the human body, characterized in that: The components include, by mass percentage: 30% to 45% of hydrolyzed whey protein powder, 20% to 25% of soy oligopeptides, 15% to 22% of bovine collagen peptides, and 8% to 15% of composite active peptides; The composite active peptide comprises at least one first type peptide that activates the small intestinal oligopeptide transporter PEPT1 and at least one second type peptide that inhibits the activity of dipeptidyl peptidase-IV.

2. The dietary protein human supplement that is easily absorbed by the human body according to claim 1, characterized in that: The molecular weight of the hydrolyzed whey protein powder is ≤1000Da, the content of branched-chain amino acids in the soy oligopeptides is ≥18%, the hydroxyproline content of the bovine collagen peptide is ≥8%, and the proportion of components with a molecular weight <1000Da is ≥90%.

3. The dietary protein human supplement that is easily absorbed by the human body according to claim 1, characterized in that: The components are as follows by mass percentage: 45% hydrolyzed whey protein powder, 25% soybean oligopeptide, 22% bovine collagen peptide and 8% composite active peptide.

4. The method for preparing a dietary protein human supplement that is easily absorbed by the human body according to any one of claims 1 to 3, characterized in that: include: S1. Pre-dry the soybean oligopeptide and bovine collagen peptide at 40°C and humidity ≤15% for 2 hours to reduce the water activity to below 0.3; S2. Separately sieve the hydrolyzed whey protein powder, soy oligopeptide, bovine collagen peptide, and composite active peptide to control the particle size to 80-120 mesh; S3, adding the sieved soybean oligopeptides and bovine collagen peptides into a mixing device and mixing them to obtain a first mixed material; S4, adding the screened composite active peptide to the first mixed material and mixing them to obtain a second mixed material; S5. Add the sieved hydrolyzed whey protein powder to the second mixed material and mix them to obtain a mixed product; S6. After screening the mixed product, the mixed product is crushed by liquid nitrogen co-flow to obtain a dietary protein human supplement.

5. The method for preparing a dietary protein human supplement that is easily absorbed by the human body according to claim 4, characterized in that: The screened soybean oligopeptides and bovine collagen peptides are added to a mixing device for mixing at a temperature of 25-30° C., a humidity of ≤25%, a rotation speed of 150-200 r / min, a mixing time of 10-15 minutes, and a pH of 6.2-6.

4.

6. The method for preparing a dietary protein human supplement that is easily absorbed by the human body according to claim 4, characterized in that: The sieved composite active peptide is added to the first mixed material for mixing at a temperature of 25-30° C., a humidity of ≤25%, a rotation speed of 200-250 r / min, and a mixing time of 8-10 minutes.

7. The method for preparing a dietary protein human supplement that is easily absorbed by the human body according to claim 4, characterized in that: The sieved hydrolyzed whey protein powder is added to the second mixed material for mixing at a temperature of 20-25° C., a humidity of ≤30%, a rotation speed of 300-350 r / min, and a mixing time of 15-20 minutes.

8. The method for preparing a dietary protein human supplement that is easily absorbed by the human body according to claim 4, characterized in that: The liquid nitrogen accompanying flow pulverization temperature is -20°C, and the particle size of the powder after pulverization is controlled to be 15-50 μm.