Composition containing whitening and brightening lipidosome as well as preparation method and application of composition

Abstract

The invention relates to a composition containing whitening and brightening lipidosome and a preparation method and application thereof, and belongs to the technical field of cosmetics. Glycyrrhiza glabra and centella asiatica extracts are extracted through a compound fermentation technology, the molecular weight of the extracts is reduced to 500-1000 Da through synergistic fermentation of lactic acid bacteria, saccharomycetes and bacillus subtilis, the extracts, lecithin, cholesterol, nicotinamide and the like form lipidosome, and the lipidosome is mixed to obtain the composition containing the whitening and brightening lipidosome. According to the preparation method of the liposome, the stable liposome with the particle size of 100-200nm is formed by combining thin film dispersion with homogenization treatment. After the composition is subjected to illumination storage at 45 DEG C for 60 days, the retention rate of active ingredients is greater than or equal to 90%, the inhibition rate of tyrosinase reaches 85.6%, and the transdermal absorption amount of nicotinamide is increased by 15-20% compared with that of a traditional process. The composition can be used for preparing whitening cosmetics such as essence, and has the advantages of high stability and remarkable effect.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and relates to a composition containing whitening and brightening liposomes and a preparation method and application thereof. Background Art

[0002] In the cosmetics industry, whitening and brightening products have long been a hot topic. While existing whitening ingredients, such as vitamin C and niacinamide, offer significant whitening effects, they generally suffer from poor stability. They are easily degraded during storage due to changes in light, temperature, or pH, leading to decreased efficacy. Furthermore, most whitening ingredients have a large molecular weight and low transdermal absorption efficiency, making it difficult for them to effectively reach the deeper layers of the skin and exert their effects. This results in a discrepancy between the actual product effects and theoretical expectations.

[0003] At the same time, plant extracts have attracted much attention in the whitening field due to their natural and mild properties. However, traditional solvent-based extraction methods have complex compositions and large molecular weights, resulting in poor compatibility with liposomes. These extracts are prone to stratification and precipitation, and the active ingredients are susceptible to oxidation and degradation under light and high temperature conditions, further affecting product stability and user experience. Therefore, it is of great significance to develop an extraction process that can reduce the molecular weight of plant extracts, enhance compatibility with liposomes, and improve stability. Summary of the Invention

[0004] The present invention aims to provide a composition containing whitening and brightening liposomes, as well as its preparation method and application. By using a composite fermentation extraction process to prepare plant extracts and optimizing the liposome formulation and preparation process, this approach addresses the problems of poor compatibility and instability between plant extracts and liposomes in existing whitening products, achieving efficient and stable delivery of whitening ingredients.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A composition containing whitening and brightening liposomes, the composition comprising a whitening and brightening liposome component, a composite plant extract component, a moisturizing component, a pH adjusting component and a solvent, wherein:

[0007] The whitening and brightening liposome components include, by weight: 10-20 parts of lecithin, 5-10 parts of cholesterol, 5-15 parts of niacinamide, and 1-3 parts of vitamin E acetate;

[0008] The composite plant extract components include, by mass: 3-8 parts of Glycyrrhiza glabra extract and 2-6 parts of Centella asiatica extract;

[0009] The moisturizing component comprises, by mass, 5-15 parts of glycerin and 0.1-0.5 parts of hyaluronic acid;

[0010] The pH adjusting component includes, by mass: 0.1-0.5 parts of triethanolamine;

[0011] The solvent is deionized water, and the balance is .

[0012] As a preferred technical solution of the present invention, in the whitening and brightening liposome component, the mass ratio of lecithin to cholesterol is (2-3):1, the mass ratio of niacinamide to Glycyrrhiza glabra extract is (1-2):1, and the mass ratio of Glycyrrhiza glabra extract to Centella asiatica extract is (1-1.5):1.

[0013] As a preferred technical solution of the present invention, the Glycyrrhiza glabra extract and the Centella asiatica extract are both prepared by a composite fermentation extraction process, and the specific steps are as follows:

[0014] Raw material pretreatment: crush the plant raw material to 80-100 mesh, add deionized water at a material-liquid ratio of 1:(5-8), adjust the pH to 4.5-5.5, add the composite fermentation liquid, and the inoculation amount is 5%-8% by volume;

[0015] The composite fermentation liquid is a mixture of lactic acid bacteria, yeast, and Bacillus subtilis at a concentration of 2:1:1, and the total bacterial concentration is ≥1×10 8 CFU / mL;

[0016] Fermentation conditions: Ferment at a constant temperature of 30-35°C for 48-72 hours, stirring every 12 hours, and a rotation speed of 50-80 r / min;

[0017] Post-treatment: the fermentation broth is centrifuged at 3000 r / min for 15 minutes to remove the residue, the supernatant is filtered through a 0.45 μm filter membrane, and concentrated under reduced pressure to 1 / 5-1 / 3 of the original volume to obtain the composite fermented plant extract.

[0018] The present invention also provides a method for preparing the above-mentioned whitening and brightening liposome composition, comprising the following steps:

[0019] S1: Prepare liposome membrane material solution: add lecithin, cholesterol and vitamin E acetate to anhydrous ethanol and stir at 50-70°C until completely dissolved to obtain liposome membrane material solution;

[0020] S2: preparing an aqueous phase solution: adding niacinamide, glycerin, hyaluronic acid, and deionized water into a container, stirring at 40-60° C. until completely dissolved, and adjusting the pH to 5.5-6.5 with triethanolamine to obtain an aqueous phase solution;

[0021] S3: Preparation of liposomes by thin film dispersion method: Pour the liposome film material solution obtained in step S1 into a flask of a rotary evaporator, and perform rotary evaporation at 50-70°C, a pressure of -0.08 to -0.06 MPa, and a rotation speed of 30-50 r / min until a uniform lipid film is formed on the inner wall of the flask. Then, add the aqueous phase solution obtained in step S2, and ultrasonically vibrate at 40-60°C for 30-60 minutes to fully hydrate the lipid film to form a liposome suspension;

[0022] S4: Homogenization: Transfer the liposome suspension obtained in step S3 to a homogenizer, homogenize 1-2 times at a pressure of 10-20 MPa and 40-50° C., add Glycyrrhiza glabra extract and Centella asiatica extract and mix to obtain a composition containing whitening and brightening liposomes.

[0023] As a preferred technical solution of the present invention, the particle size of the liposome is 100-200 nm.

[0024] The present invention also provides use of the whitening and brightening liposome composition in preparing whitening and brightening cosmetics, including but not limited to essences, emulsions, and creams.

[0025] Beneficial effects of the present invention:

[0026] (1) The present invention prepares plant extracts through a composite fermentation extraction process, utilizes microbial enzymatic hydrolysis to decompose plant cell walls, and reduces the average molecular weight of Glycyrrhiza glabra extract and Centella asiatica extract to 500-1000 Da, forming a good match with the nanoscale particle size of liposomes, significantly improving the compatibility of plant extracts and liposomes, reducing the occurrence of stratification and precipitation, and enhancing the physical stability of the composition.

[0027] (2) The organic acids (such as lactic acid) and small molecule peptides produced during the composite fermentation process can be adsorbed on the surface of liposomes, increasing the charge stability of the liposomes. At the same time, the antioxidant components in the fermentation products can effectively inhibit the oxidative degradation of the whitening components. After the composition is stored under light conditions at 45°C for 60 days, the retention rates of glycyrrhizin and asiaticoside are both ≥90%, which is significantly better than the traditional solvent extraction method.

[0028] (3) The nano-sized particle size of the liposomes and the small molecular size of the plant extracts work together to significantly improve the transdermal absorption efficiency of the whitening ingredients. Experiments have shown that the transdermal absorption rate of niacinamide in the composition of the present invention is 15%-20% higher than that of the traditional solvent extraction method, and the transdermal absorption rate of Glycyrrhiza glabra extract and Centella asiatica extract is increased by 10%-15%, thereby further enhancing the whitening and brightening effects. DETAILED DESCRIPTION

[0029] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the specific implementation methods, structures, characteristics and effects of the present invention are described in detail below in combination with the embodiments. Unless otherwise specified, the raw materials involved below are all commercially available.

[0030] Example 1

[0031] A composition containing whitening and brightening liposomes, comprising the following components:

[0032] Components content Lecithin 15 servings cholesterol 7.5 servings Niacinamide 10 servings Glycyrrhiza glabra extract (complex fermentation method) 5 servings Centella asiatica extract (complex fermentation method) 4 servings Vitamin E acetate 2 servings glycerin 10 servings Hyaluronic acid 0.3 parts triethanolamine 0.3 parts Deionized water Make up to 100 copies

[0033] The Glycyrrhiza glabra extract and Centella asiatica extract are both prepared by a composite fermentation extraction process, and the specific steps are as follows:

[0034] Raw material pretreatment: crush the plant raw material into 80 mesh, add deionized water at a material-liquid ratio of 1:5, adjust the pH to 4.5, add the composite fermentation liquid, and the inoculation amount is 5% by volume;

[0035] The composite fermentation liquid is a mixture of lactic acid bacteria, yeast, and Bacillus subtilis at a concentration of 2:1:1, with a total bacterial concentration of 2×10 8 CFU / mL;

[0036] Fermentation conditions: Fermentation was carried out at a constant temperature of 30°C for 72 hours, with stirring every 12 hours and a rotation speed of 50 r / min.

[0037] Post-treatment: the fermentation broth was centrifuged at 3000 r / min for 15 minutes to remove the residue, the supernatant was filtered through a 0.45 μm filter membrane, and concentrated under reduced pressure to 1 / 5 of the original volume to obtain the composite fermented plant extract.

[0038] The preparation method of the above-mentioned whitening and brightening liposome composition comprises the following steps:

[0039] S1: Prepare liposome membrane material solution: add lecithin, cholesterol and vitamin E acetate to anhydrous ethanol and stir at 50°C until completely dissolved to obtain liposome membrane material solution;

[0040] S2: preparing an aqueous phase solution: adding niacinamide, glycerin, hyaluronic acid, and deionized water into a container, stirring at 40°C until completely dissolved, and adjusting the pH to 5.5 with triethanolamine to obtain an aqueous phase solution;

[0041] S3: Preparation of liposomes by thin film dispersion method: Pour the liposome film material solution obtained in step S1 into a flask of a rotary evaporator, and rotary evaporate at 50°C, pressure -0.08 MPa, and rotation speed 30 r / min until a uniform lipid film is formed on the inner wall of the flask. Then, add the aqueous phase solution obtained in step S2, and ultrasonically oscillate at 40°C for 30 minutes to fully hydrate the lipid film to form a liposome suspension;

[0042] S4: Homogenization: The liposome suspension obtained in step S3 is transferred to a homogenizer and homogenized twice at a pressure of 10 MPa and 40° C., and the extract of Glycyrrhiza glabra and the extract of Centella asiatica are added and mixed to obtain a composition containing whitening and brightening liposomes.

[0043] Example 2

[0044]

[0045]

[0046] The Glycyrrhiza glabra extract and Centella asiatica extract are both prepared by a composite fermentation extraction process, and the specific steps are as follows:

[0047] Raw material pretreatment: crush the plant raw materials into 90 mesh, add deionized water at a material-liquid ratio of 1:6, adjust the pH to 5.0, add the composite fermentation liquid, and the inoculation amount is 6% by volume;

[0048] The composite fermentation liquid is a mixture of lactic acid bacteria, yeast, and Bacillus subtilis at a concentration of 2:1:1, with a total bacterial concentration of 2×10 8 CFU / mL;

[0049] Fermentation conditions: Fermentation was carried out at a constant temperature of 35°C for 48 hours, with stirring every 12 hours and a rotation speed of 80 r / min.

[0050] Post-treatment: the fermentation broth was centrifuged at 3000 r / min for 15 minutes to remove the residue, the supernatant was filtered through a 0.45 μm filter membrane, and concentrated under reduced pressure to 1 / 3 of the original volume to obtain the composite fermented plant extract.

[0051] The preparation method of the above-mentioned whitening and brightening liposome composition comprises the following steps:

[0052] S1: Prepare liposome membrane material solution: add lecithin, cholesterol and vitamin E acetate to anhydrous ethanol and stir at 60°C until completely dissolved to obtain liposome membrane material solution;

[0053] S2: preparing an aqueous phase solution: adding niacinamide, glycerin, hyaluronic acid, and deionized water into a container, stirring at 50°C until completely dissolved, and adjusting the pH to 6.5 with triethanolamine to obtain an aqueous phase solution;

[0054] S3: Preparation of liposomes by thin film dispersion method: Pour the liposome film material solution obtained in step S1 into a flask of a rotary evaporator, and rotary evaporate at 60°C, pressure -0.06 MPa, and rotation speed 40 r / min until a uniform lipid film is formed on the inner wall of the flask. Then, add the aqueous phase solution obtained in step S2, and ultrasonically oscillate at 50°C for 40 minutes to fully hydrate the lipid film to form a liposome suspension;

[0055] S4: Homogenization: The liposome suspension obtained in step S3 is transferred to a homogenizer and homogenized twice at a pressure of 20 MPa and 50° C., and the extract of Glycyrrhiza glabra and the extract of Centella asiatica are added and mixed to obtain a composition containing whitening and brightening liposomes.

[0056] Example 3

[0057] Components content Lecithin 18 servings cholesterol 9 servings Niacinamide 12 servings Glycyrrhiza glabra extract (complex fermentation method) 6 servings Centella asiatica extract (complex fermentation method) 5 servings Vitamin E acetate 2.5 servings glycerin 12 servings Hyaluronic acid 0.4 serving triethanolamine 0.4 serving Deionized water Make up to 100 copies

[0058] The Glycyrrhiza glabra extract and Centella asiatica extract are both prepared by a composite fermentation extraction process, and the specific steps are as follows:

[0059] Raw material pretreatment: crush the plant raw material to 100 mesh, add deionized water at a material-liquid ratio of 1:8, adjust the pH to 5.5, add the composite fermentation liquid, and the inoculation amount is 8% by volume;

[0060] The composite fermentation liquid is a mixture of lactic acid bacteria, yeast, and Bacillus subtilis at a concentration of 2:1:1, with a total bacterial concentration of 2×10 8 CFU / mL;

[0061] Fermentation conditions: Fermentation was carried out at a constant temperature of 35°C for 48 hours, with stirring every 12 hours and a rotation speed of 80 r / min.

[0062] Post-treatment: the fermentation broth was centrifuged at 3000 r / min for 15 minutes to remove the residue, the supernatant was filtered through a 0.45 μm filter membrane, and concentrated under reduced pressure to 1 / 3 of the original volume to obtain the composite fermented plant extract.

[0063] The preparation method of the above-mentioned whitening and brightening liposome composition comprises the following steps:

[0064] S1: Prepare liposome membrane material solution: add lecithin, cholesterol and vitamin E acetate to anhydrous ethanol and stir at 70°C until completely dissolved to obtain liposome membrane material solution;

[0065] S2: preparing an aqueous phase solution: adding niacinamide, glycerin, hyaluronic acid, and deionized water into a container, stirring at 60° C. until completely dissolved, and adjusting the pH to 6.5 with triethanolamine to obtain an aqueous phase solution;

[0066] S3: Preparation of liposomes by thin film dispersion method: Pour the liposome film material solution obtained in step S1 into a flask of a rotary evaporator, and rotary evaporate at 70°C, pressure -0.06 MPa, and rotation speed 50 r / min until a uniform lipid film is formed on the inner wall of the flask. Then, add the aqueous phase solution obtained in step S2, and ultrasonically oscillate at 60°C for 60 minutes to fully hydrate the lipid film to form a liposome suspension;

[0067] S4: Homogenization: The liposome suspension obtained in step S3 is transferred to a homogenizer and homogenized twice at a pressure of 20 MPa and 50° C., and the extract of Glycyrrhiza glabra and the extract of Centella asiatica are added and mixed to obtain a composition containing whitening and brightening liposomes.

[0068] Comparative Example 1

[0069] The difference from Example 1 is that no Glycyrrhiza glabra extract and Centella asiatica extract were added.

[0070] Comparative Example 2

[0071] The difference from Example 1 is that nicotinamide is not added.

[0072] Comparative Example 3

[0073] The difference from Example 1 is that no cholesterol was added.

[0074] Comparative Example 4

[0075] The difference from Example 1 is that lecithin is not added, and only cholesterol is used as the liposome membrane material.

[0076] Comparative Example 5

[0077] The difference from Example 1 is that no hyaluronic acid is added.

[0078] Comparative Example 6

[0079] The difference from Example 1 is that the liposome preparation step is not performed and all components are directly mixed and stirred evenly.

[0080] Comparative Example 7

[0081] The difference from Example 1 is that the Glycyrrhiza glabra extract and the Centella asiatica extract are prepared by the following steps:

[0082] Glycyrrhiza glabra extract: add 60% ethanol solution by volume at a solid-liquid ratio of 1:10, extract twice by ultrasonic at 50°C for 1.5 hours each time, and concentrate under reduced pressure until there is no ethanol smell to obtain the extract;

[0083] Centella asiatica extract: add 50% methanol solution by volume at a solid-liquid ratio of 1:8, reflux extraction at 60°C twice, each time for 2 hours, and concentrate to dryness under reduced pressure to obtain the extract.

[0084] Comparative Example 8

[0085] The difference from Example 1 is that the Glycyrrhiza glabra extract is replaced by arbutin extract, and the corresponding extraction method remains unchanged.

[0086] Comparative Example 9

[0087] The difference from Example 1 is that the Centella asiatica extract is replaced by the Scutellaria baicalensis extract, and the corresponding extraction method remains unchanged.

[0088] Comparative Example 10

[0089] The difference from Example 1 is that a single lactic acid bacteria fermentation is used.

[0090] Comparative Example 11

[0091] The difference from Example 1 is that the ratio of lactic acid bacteria to yeast is 1:1, and Bacillus subtilis is absent.

[0092] Comparative Example 12

[0093] The difference from Example 1 is that the ratio of lactic acid bacteria to Bacillus subtilis is 3:1, and there is no yeast.

[0094] Comparative Example 13

[0095] The difference from Example 1 is that the ratio of lactic acid bacteria: yeast: Bacillus subtilis is 1:1:1.

[0096] Performance testing

[0097] 1. Stability test

[0098] 1. Long-term storage stability test

[0099] Instruments: Constant temperature and humidity chamber, precision pH meter (Mettler S220), UV-visible spectrophotometer (Shimadzu UV-2600)

[0100] Steps:

[0101] Sample storage: Place 50 mL of sample into a brown glass bottle, seal it, and place it in a constant temperature box at 25 ± 0.5 °C and 60 ± 3% RH, away from light.

[0102] pH value measurement (once a month):

[0103] The instrument was calibrated with pH 4.01, 7.00, and 10.01 standard buffers;

[0104] Take 5 mL of sample, insert the electrode, and record the data when the reading stabilizes (≤0.01 pH / min change);

[0105] Light transmittance measurement (once a month):

[0106] The sample was diluted 10-fold with deionized water and degassed by ultrasonication for 5 min;

[0107] Use a 1 cm quartz cuvette at a wavelength of 600 nm with deionized water as a blank to measure the absorbance A, transmittance = 10 -A ×100%.

[0108] 2.45℃ Light Stability Test

[0109] instrument:

[0110] Illumination incubator, high performance liquid chromatography (Agilent 1260)

[0111] Steps:

[0112] Sample storage: 50 mL of sample was placed in a transparent glass bottle and placed in an incubator at 45 ± 0.5 °C and a light intensity of 4500 lx for 60 days;

[0113] Active ingredient determination (days 0, 30, and 60):

[0114] Sample pretreatment: Take 1 mL of sample, dilute 10 times with mobile phase, and filter through a 0.22 μm filter membrane;

[0115] HPLC conditions:

[0116] Chromatographic column: Agilent ZORBAX SB-C18 (250 mm × 4.6 mm, 5 μm)

[0117] Mobile phase: methanol-0.1% phosphoric acid aqueous solution (60:40, v / v), pH adjusted to 3.0 with phosphoric acid

[0118] Flow rate: 1.00 mL / min, column temperature 30.0 ± 0.5 °C, injection volume 10 μL

[0119] Detection wavelength: 276nm for glycyrrhizin, 205nm for asiaticoside

[0120] Standard curve: Prepare standard solutions of glycyrrhizin glabra (10-100 μg / mL) and asiaticoside (5-50 μg / mL) and draw an external standard curve with R2 ≥ 0.999;

[0121] Calculation of retention rate: retention rate = (concentration after storage / initial concentration) × 100%, accurate to 0.1%.

[0122] Table 1 Stability test results

[0123]

[0124]

[0125] 2. Tyrosinase inhibition rate test

[0126] instrument:

[0127] Microplate reader (BioTek Synergy H1), constant temperature water bath

[0128] Reagents:

[0129] Tyrosinase (Sigma, activity 2000 U / mg, prepared to 2 U / mL in PBS), L-dopa (Sigma, prepared to 2 mmol / L in PBS, pH 6.8), phosphate buffered saline (PBS, 0.1 mol / L, pH 6.8, filtered through a 0.22 μm filter)

[0130] Steps:

[0131] Sample preparation: Take 1 mL of sample, dilute 10 times with PBS, and mix by ultrasonication for 30 seconds;

[0132] Reaction system (200 μL / well, 3 replicates):

[0133]

[0134] Reaction conditions: incubate at 37.0±0.5℃ for 10 minutes, immediately measure the absorbance at 475nm, and read the OD value (accurate to 0.001);

[0135] Inhibition rate calculation: Inhibition rate (%) = [1-(OD 测试 -OD 空白 ) / (OD 对照 -OD 空白 )]×100%

[0136] The results were averaged over 3 replicates.

[0137] Table 2 Tyrosinase inhibition rate test results

[0138]

[0139]

[0140] 3. Nicotinamide transdermal absorption test (Franz diffusion cell method)

[0141] instrument:

[0142] Upright Franz diffusion cell (effective diffusion area 1.76 cm2, receiving cell volume 5 mL), constant temperature water bath circulation system, magnetic stirrer, high performance liquid chromatography (Agilent 1260)

[0143] Experimental Materials:

[0144] Fresh pig skin (within 24 hours after slaughter, remove subcutaneous fat, wash with saline, and store at -20℃ for no more than 1 week)

[0145] Steps:

[0146] Pigskin pretreatment: Take out the frozen pigskin, thaw it at room temperature, gently scrape off the debris on the stratum corneum with a scalpel, and fix it with a Franz diffusion cell clamp, with the stratum corneum facing the supply cell;

[0147] Transdermal experiment:

[0148] Supply cell: add 2.00 mL of sample and cover with plastic wrap to prevent evaporation;

[0149] Receiver cell: Add 5.00 mL of pH 7.4 phosphate buffer (containing 0.1% sodium azide for preservation) and stir magnetically;

[0150] Sampling time points: 1, 2, 4, 6, and 8 hours, taking 1.00 mL of receiving solution each time (simultaneously adding 1.00 mL of preheated buffer);

[0151] HPLC detection of nicotinamide:

[0152] Sample pretreatment: The receiving solution was filtered through a 0.22 μm filter membrane;

[0153] Chromatographic conditions:

[0154] Chromatographic column: Agilent Eclipse Plus C18 (150 mm × 4.6 mm, 5 μm)

[0155] Mobile phase: methanol-0.05 mol / L potassium dihydrogen phosphate (5:95, v / v), adjust pH to 3.0 with phosphoric acid. Flow rate: 1.00 mL / min, column temperature 30.0°C, injection volume 20 μL

[0156] Detection wavelength: 261nm

[0157] Standard curve: prepare nicotinamide standard solution (10-200 μg / mL), R2 ≥ 0.999;

[0158] Calculation of cumulative transdermal absorption:

[0159]

[0160] Where: C i is the i-th sampling concentration (μg / mL), V is the volume of the receiving cell (5 mL), ΔV is the sampling volume (1 mL), and A is the diffusion area (1.76 cm2).

[0161] Table 3 Nicotinamide transdermal absorption test results

[0162] Time (h) Comparative Example 6 Example 1 1 7.0 12.5 2 12.0 22.8 4 20.0 38.5 6 28.0 50.2 8 35.0 62.5

[0163] 4. Liposome Particle Size and Zeta Potential Detection

[0164] instrument:

[0165] Dynamic light scattering instrument (Malvern Zetasizer Nano ZS), ultrasonic processor (power 300W, frequency 40kHz)

[0166] Steps:

[0167] Sample preparation: 1 mL of the composition was diluted 10-fold with ultrapure water (18.2 MΩ·cm) and sonicated for 30 seconds (power 30%, pulse mode: on 5 seconds / off 5 seconds) to eliminate agglomerates.

[0168] Particle size determination:

[0169] Preheat the instrument for 30 minutes and rinse the sample cell three times with ultrapure water;

[0170] Inject 1.0 mL of diluted sample and equilibrate at 25.0 ± 0.1 °C for 60 seconds;

[0171] Dynamic light scattering (DLS) was used, and the measurements were repeated three times, each lasting 120 seconds. The average particle size (Z-average) and polydispersity index (PDI) were automatically calculated. A PDI ≤ 0.3 was considered a monodisperse system.

[0172] Zeta potential determination:

[0173] Replace the sample cell with a Zeta potential dedicated sample cell and inject 1.0 mL of sample;

[0174] Apply an electric field strength of 150 V / m and balance for 30 seconds;

[0175] The measurement was repeated five times, each lasting 90 seconds, and the average value and standard deviation of the zeta potential were calculated. The system was considered stable if the absolute value was ≥30 mV.

[0176] Table 4 Initial particle size and Zeta potential test results

[0177]

[0178] Table 5 Particle size change and Zeta potential fluctuation test results after 60 days of storage

[0179]

[0180] 5. Molecular weight detection of compound fermentation extract

[0181] instrument:

[0182] Gel permeation chromatograph (Waters 1525, differential refractive index detector 2414), chromatographic column thermostat, reagents:

[0183] Mobile phase: 0.1 mol / L sodium nitrate aqueous solution (containing 0.02% sodium azide, filtered through a 0.22 μm filter membrane, and ultrasonically degassed for 30 minutes)

[0184] Standards: Dextran standards (Mw = 1000, 5000, 10000, 20000 Da, Sigma)

[0185] Steps:

[0186] Standard curve preparation:

[0187] Weigh 10 mg of each standard, dissolve it in mobile phase and make up to 10 mL, then filter it through a 0.22 μm filter membrane.

[0188] Inject 50 μL of sample, record the retention time, and plot a standard curve using log(Mw) versus retention time. 2 ≥0.995;

[0189] Sample determination:

[0190] Take 10 mg of each composite fermentation extract, dissolve it in mobile phase and make up to 10 mL, and filter it through a 0.22 μm filter membrane;

[0191] Chromatographic conditions:

[0192] Chromatographic column: Shodex OHpak SB-804HQ (300 mm × 8 mm, 6 μm)

[0193] Mobile phase flow rate: 0.80 mL / min, column temperature 40.0 °C, injection volume 50 μL

[0194] The sample retention time was recorded and the weight-average molecular weight (Mw) was calculated according to the standard curve. The result was the average of three repetitions with an error of ≤3%.

[0195] Table 6 Molecular weight test results of composite fermentation extract

[0196]

[0197]

[0198] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can, without departing from the scope of the technical solution of the present invention, make some changes or modifications to equivalent embodiments using the technical contents disclosed above. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A composition containing whitening and brightening liposomes, characterized in that: The composition comprises a whitening and brightening liposome component, a composite plant extract component, a moisturizing component, a pH regulating component and a solvent, wherein: The whitening and brightening liposome components include, by weight: 10-20 parts of lecithin, 5-10 parts of cholesterol, 5-15 parts of niacinamide, and 1-3 parts of vitamin E acetate; The composite plant extract components include, by mass: 3-8 parts of Glycyrrhiza glabra extract and 2-6 parts of Centella asiatica extract; The moisturizing component comprises, by mass, 5-15 parts of glycerin and 0.1-0.5 parts of hyaluronic acid; The pH adjusting component includes, by mass: 0.1-0.5 parts of triethanolamine; The solvent is deionized water, and the balance is .

2. The composition containing whitening and brightening liposomes according to claim 1, characterized in that: In the whitening and brightening liposome component, the mass ratio of lecithin to cholesterol is (2-3):1, the mass ratio of niacinamide to Glycyrrhiza glabra extract is (1-2):1, and the mass ratio of Glycyrrhiza glabra extract to Centella asiatica extract is (1-1.5):

1.

3. The composition containing whitening and brightening liposomes according to claim 1, characterized in that: The Glycyrrhiza glabra extract and Centella asiatica extract are both prepared by a composite fermentation extraction process, and the specific steps are as follows: Raw material pretreatment: crush the plant raw material to 80-100 mesh, add deionized water at a material-liquid ratio of 1:(5-8), adjust the pH to 4.5-5.5, add the composite fermentation liquid, and the inoculation amount is 5%-8% by volume; The composite fermentation liquid is a mixture of lactic acid bacteria, yeast, and Bacillus subtilis at a concentration of 2:1:1, and the total bacterial concentration is ≥1×10 8 CFU / mL; Fermentation conditions: Ferment at a constant temperature of 30-35°C for 48-72 hours, stirring every 12 hours, and a rotation speed of 50-80 r / min; Post-treatment: the fermentation broth is centrifuged at 3000 r / min for 15 minutes to remove the residue, the supernatant is filtered through a 0.45 μm filter membrane, and concentrated under reduced pressure to 1 / 5-1 / 3 of the original volume to obtain the composite fermented plant extract.

4. A method for preparing the whitening and brightening liposome composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1: Prepare liposome membrane material solution: add lecithin, cholesterol and vitamin E acetate to anhydrous ethanol and stir at 50-70°C until completely dissolved to obtain liposome membrane material solution; S2: preparing an aqueous phase solution: adding niacinamide, glycerin, hyaluronic acid, and deionized water into a container, stirring at 40-60° C. until completely dissolved, and adjusting the pH to 5.5-6.5 with triethanolamine to obtain an aqueous phase solution; S3: Preparation of liposomes by thin film dispersion method: Pour the liposome film material solution obtained in step S1 into a flask of a rotary evaporator, and rotary evaporate at 50-70°C until a uniform lipid film is formed on the inner wall of the flask. Then, add the aqueous phase solution obtained in step S2, and ultrasonically oscillate at 40-60°C for 30-60 minutes to fully hydrate the lipid film to form a liposome suspension; S4: Homogenization: The liposome suspension obtained in step S3 is transferred to a homogenizer and homogenized 1-2 times at a pressure of 10-20 MPa, and the Glycyrrhiza glabra extract and the Centella asiatica extract are added and mixed to obtain a composition containing whitening and brightening liposomes.

5. The preparation method according to claim 4, characterized in that The pressure of the rotary evaporation in step S3 is -0.08 to -0.06 MPa, and the rotation speed is 30-50 r / min.

6. The preparation method according to claim 4, characterized in that The temperature of the homogenization treatment in step S4 is 40-50°C.

7. Use of the whitening and brightening liposome composition according to any one of claims 1 to 3 in the preparation of whitening and brightening cosmetics, characterized in that: The cosmetics include essence, lotion or cream.

8. The composition containing whitening and brightening liposomes according to claim 1, characterized in that: The particle size of the liposome is 100-200 nm.

9. The composition containing whitening and brightening liposomes according to claim 3, characterized in that: The addition of the composite fermentation extract ensures that after the composition is stored at 45° C. for 60 days under light conditions, the retention rates of glycyrrhizin glabra and asiaticoside are both ≥90%, and the change in liposome particle size is ≤10%.

10. The composition containing whitening and brightening liposomes according to claim 1, characterized in that: After the composition is stored at room temperature for 6 months, the pH value changes by no more than 0.5, and the light transmittance decreases by no more than 15%.

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