Oat refined oil extract, and preparation method and application thereof

Abstract

The present application provides a kind of oat refined oil extract and its preparation method and application, it is related to cosmetic technical field.The ingredients of oat refined oil extract include 88-92 parts of oat kernel oil and 8-12 parts of oat bran extract according to weight parts;(1) oat bran is mixed with ethanol-n-hexane-limonene, microwave treatment is obtained oat bran residue;(2) oat bran residue is extracted by supercritical carbon dioxide, and the entrainer is ethanol-n-hexane-limonene;The extraction condition is 35-45 DEG C, 50-60 MPa, static extraction is 50-80 min;(3) extract solution is combined, and oat bran extract is obtained.Oat kernel is pressed to obtain oat kernel oil A and oat residue;Oat residue is extracted by supercritical carbon dioxide, and oat kernel oil B is obtained;The oat kernel oil of two times is combined.The yield of oat bran extract and oat kernel oil of the present application is high, has significantly improved moisturizing, anti-inflammatory effect, and can be applied in cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an oat refined oil extract, its preparation method, and its application. Background Technology

[0002] Oats belong to the Poaceae family, Aves section, and Aves genus. Extracting oat oil and active ingredients from oats can increase their added value. Oat seeds are enclosed in two outer shells, with the seed surface covered by fine hairs. The seed coat, or bran, contains protein, lipids, and cellulose. The remaining parts of the seed are the endosperm and germ, with the endosperm making up the largest proportion. The endosperm contains protein, starch, lipids, and cellulose. In oat lipids, palmitic acid, oleic acid, and linoleic acid account for over 95% of the total fatty acids. It also contains lauric acid, palmitoleic acid, arachidonic acid, eicosine unsaturated fatty acids, trace amounts of lignin and nervonic acid. Oat oil not only contains high-quality fatty acids but is also rich in polyphenols, sterols, tocopherols, and other active ingredients. These can scavenge free radicals, providing anti-aging, moisturizing, and anti-inflammatory effects. Oat oil has wide applications in the cosmetics, pharmaceutical, and food industries.

[0003] Chinese invention patent CN101787059A discloses a method for extracting digalactosyl diglyceride from oats. The preparation process is as follows: oat bran is defatted by supercritical carbon dioxide extraction, the defatted oat bran is extracted with acetone, the extract is concentrated under reduced pressure, and adsorbed with 1-3 times its weight of silica gel. Blank silica gel is added to the separation column first, followed by silica gel with the sample, with a blank silica gel:sample silica gel weight ratio of 2-4:1. Then, elution is performed with a chloroform-methanol mixture with a volume ratio of 70-90:30-10, and finally elution is performed with a chloroform-methanol mixture with a volume ratio of 60-80:40-20. The component in the eluent is purified digalactosyl diglyceride.

[0004] Chinese invention patent CN119286583A discloses a process for preparing multiple nutrients from oats. The process includes the following steps: oat pulverization and sieving to obtain oat bran; extraction of oat bran using supercritical extraction technology to obtain pretreated oat bran and extract; extraction of the extract through gradient membrane treatment and membrane ultrafiltration to obtain oat oil; extraction of pretreated oat bran using a continuous segmented extraction device; after a first stage of protein extraction, a second stage of dextran extraction, a third stage of immobilized enzymatic hydrolysis, and a fourth stage of decolorization with packing material, the extract enters a system equilibrium tank for membrane separation and concentration to obtain oat β-glucan.

[0005] In addition, Chinese invention patent CN119606845A discloses a Centella asiatica plant extract repair agent, comprising the following components: olive leaf extract, Centella asiatica root extract, and oat kernel extract; the preparation method of the above extract components is as follows: each raw material is pulverized, cellulase is added for enzymatic hydrolysis, ethanol is added for soaking and extraction, and then filtered; then an equal volume of glycerol is added to the filtrate, the ethanol is removed by rotary evaporation, and the supernatant is collected by centrifugation; the filter residue is dried, and supercritical carbon dioxide is extracted for 2-3 hours to obtain an oily extract; the oily extract and the supernatant are mixed and homogenized by ultrasonic treatment to obtain the Centella asiatica plant extract repair agent.

[0006] Most existing oat oil extraction processes do not consider the impact of the oat material itself or its combination with other auxiliary extraction methods on the extraction effect, nor do they consider the impact on the moisturizing, anti-inflammatory, and activity effects of oat oil. Therefore, there is a need to provide a method for preparing refined oat oil that comprehensively utilizes all parts of the oat seed to improve the utilization rate of oats and enhance the moisturizing, anti-inflammatory, and antioxidant effects of oat oil extract. Summary of the Invention

[0007] This invention addresses the problems existing in the prior art by providing a refined oat oil extract, its preparation method, and its application. The refined oat oil extract obtained by separately processing oat kernel oil and oat bran extract and mixing them in a specific ratio has significantly improved moisturizing and anti-inflammatory effects. Before supercritical carbon dioxide extraction, the oat bran is first microwave-treated to improve the extract yield while ensuring that the active ingredients are not destroyed.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] First, the present invention provides an oat refined oil extract, the components of which, by weight, include: 88-92 parts oat kernel oil and 8-12 parts oat bran extract;

[0010] The preparation method of the oat bran extract includes the following steps:

[0011] (1) Mix oat bran with a mixed solution of ethanol-n-hexane-limonene and microwave at 200-400W for 2-5 minutes to obtain extraction solution A and oat bran residue;

[0012] (2) Supercritical carbon dioxide extraction was performed on oat bran residue, with a mixed solution of ethanol-n-hexane-limonene as the entrainer; the extraction temperature was 35-45℃, the extraction pressure was 50-60MPa, and the static extraction time was 50-80min to obtain extraction solution B.

[0013] (3) Mix extraction solution A and extraction solution B, concentrate under reduced pressure to separate the extract, and obtain oat bran extract;

[0014] In the mixed solution described in steps (1) and (2), the volume ratio of ethanol, n-hexane and limonene is 6-10:2-4:1-2.

[0015] Preferably, the mass ratio of oat kernel oil to oat bran extract is 90:10.

[0016] Preferably, in the preparation method of the oat bran extract, the volume ratio of ethanol, n-hexane and limonene in the mixed solution in step (1) is 8:3:2.

[0017] Preferably, in the preparation method of the oat bran extract, the microwave treatment in step (1) is performed under the following conditions: 300W microwave treatment for 3 minutes; during the microwave treatment, the microwave treatment can be performed intermittently to ensure that the total microwave treatment time is 2-5 minutes.

[0018] Preferably, in the preparation method of the oat bran extract, the solid-liquid ratio of the microwave treatment in step (1) is 1:16-25 g / mL.

[0019] More preferably, in the preparation method of the oat bran extract, the solid-liquid ratio of the microwave treatment in step (1) is 1:20 g / mL.

[0020] Preferably, in the preparation method of the oat bran extract, the volume ratio of ethanol, n-hexane and limonene in the mixed solution in step (2) is 8:3:2.

[0021] Preferably, in the preparation method of the oat bran extract, the supercritical carbon dioxide extraction in step (2) is specifically set with the following conditions: extraction temperature 40°C, extraction pressure 55 MPa, and static extraction time 60 min.

[0022] Then, the present invention provides a method for preparing the above-mentioned refined oat oil extract, comprising the following steps:

[0023] S1. Preparation of oat kernel oil: Oat kernels are pressed to obtain oat kernel oil A and oat residue; oat residue is subjected to supercritical carbon dioxide extraction to obtain oat kernel oil B; oat kernel oil A and oat kernel oil B are combined to form oat kernel oil.

[0024] S2. Mix oat kernel oil and oat bran extract to obtain refined oat oil extract.

[0025] In this invention, oat kernel oil refers to the oily component extracted from the endosperm and germ of oat seeds after the oat bran has been removed; or the oily component extracted from oat seeds. In the embodiments of the invention, oat kernel oil refers to the oily component extracted from the endosperm and germ of oat seeds after the oat bran has been removed.

[0026] Preferably, in step S1, the pressing is specifically: pressing at 35-45℃ and 50-70MPa for 20-40 minutes, repeated 1-3 times.

[0027] More preferably, in step S1, the pressing specifically involves pressing at 40°C and 62MPa for 30 minutes, repeated twice.

[0028] Preferably, in step S1, the supercritical carbon dioxide extraction specifically involves: an extraction pressure of 25-30 MPa, an extraction temperature of 26-30°C, an entrainer of ethanol, and a dynamic extraction time of 10-20 min.

[0029] More preferably, in step S1, the supercritical carbon dioxide extraction specifically involves: an extraction pressure of 28 MPa, an extraction temperature of 28°C, an entrainer of ethanol, and a dynamic extraction time of 15 min.

[0030] More preferably, in step S1, the amount of the entrainer is 200-300 mL / 100 g.

[0031] More preferably, in step S1, the amount of the entrainer is 250 mL / 100 g.

[0032] Preferably, in the preparation method of the refined oat oil extract, step S2 further includes degumming, decolorization, and deacidification treatment.

[0033] In this invention, the degumming and deacidification treatments are conventional methods: add an appropriate amount of deionized water, centrifuge to separate the oil layer; add activated carbon for decolorization; add the decolorized product to alkaline solution, stir, heat to 55°C, let stand and cool to room temperature, refrigerate at 4°C for 12 hours, centrifuge, and collect the deacidified product; heat the deacidified product to 85°C and wash with water until neutral.

[0034] Furthermore, this invention provides the application of the above-mentioned refined oat oil extract in the preparation of moisturizing and anti-inflammatory cosmetics.

[0035] Finally, the present invention provides a moisturizing and anti-inflammatory cosmetic, comprising the above-mentioned oat refined oil extract.

[0036] Preferably, the moisturizing and anti-inflammatory cosmetic may also contain excipient A; excipient A is one or more of emulsifiers, moisturizers, thickeners, skin conditioners, pH adjusters, antioxidants, skin conditioning agents, and preservatives.

[0037] Preferably, the dosage form of the moisturizing and anti-inflammatory cosmetic is a serum, lotion, cream, toner, facial cleanser, or mask.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] 1. In the preparation process of the oat bran extract of the present invention, microwave treatment is first performed to make the oat bran structure soft, which is conducive to the extraction of active ingredients by organic solvents; a specific ratio of ethanol, n-hexane and limonene is used as the treatment solvent, and the components interact with each other to ensure a high yield of extract while ensuring that the active ingredients are not destroyed, thereby improving moisturizing and anti-inflammatory properties.

[0040] 2. This invention processes oat kernel oil and oat bran extract separately and mixes them in a specific ratio to obtain a refined oat oil extract that has superior moisturizing and anti-inflammatory effects compared to products with a single ingredient or conventional formulations.

[0041] 3. This invention uses mild processing conditions, employing lower pressing and extraction temperatures and shorter extraction times to obtain an extract with a high yield, high content of active ingredients in the oat refined oil extract, and a light color. Detailed Implementation

[0042] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0043] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0044] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0045] Example 1

[0046] An oat refined oil extract comprises, by weight, 90 parts oat kernel oil and 10 parts oat bran extract.

[0047] The preparation method of oat bran extract is as follows:

[0048] (1) Mix oat bran with a mixed solution of ethanol-n-hexane-limonene in a volume ratio of 8:3:2. The solid-liquid ratio after mixing is 1:20 g / mL. Perform intermittent microwave treatment at 300W power for 3 min (intermittent once every 1 min). Filter to obtain extract solution A and oat bran residue.

[0049] (2) Oat bran residue was subjected to supercritical carbon dioxide extraction with a mixed solution of ethanol-n-hexane-limonene in a volume ratio of 8:3:2. The extraction temperature was 40℃, the extraction pressure was 55MPa, and the static extraction time was 60min to obtain extraction solution B.

[0050] (3) Mix extraction solution A and extraction solution B, concentrate under reduced pressure to separate the extract, and obtain oat bran extract.

[0051] The preparation method of refined oat oil extract is as follows:

[0052] S1. Preparation of oat kernel oil: Oat kernels were pressed at 40℃ and 62MPa for 30 minutes, repeated twice, and filtered to obtain oat kernel oil A and oat residue;

[0053] Oat residue was subjected to supercritical carbon dioxide extraction at an extraction pressure of 28 MPa, an extraction temperature of 28 °C, an entrainer of ethanol (250 mL / 100 g), and a dynamic extraction time of 15 min to obtain oat kernel oil B.

[0054] Oat kernel oil A and oat kernel oil B are combined into oat kernel oil;

[0055] S2. Add oat kernel oil to an appropriate amount of deionized water at the same temperature, centrifuge to separate the oil layer; add activated carbon for decolorization; add the decolorized product to an alkaline solution with pH=8, stir, heat to 55℃, let stand and cool to room temperature, refrigerate at 4℃ for 12 hours, centrifuge, and collect the deacidified product; heat the deacidified product to 85℃ and wash with water until neutral, then separate the oat kernel oil after washing.

[0056] Oat kernel oil and oat bran extract are mixed to obtain refined oat oil extract.

[0057] Example 2

[0058] Unlike Example 1, the weight ratio of oat kernel oil and oat bran extract in the oat refined oil extract is different.

[0059] An oat refined oil extract comprises, by weight, 88 parts oat kernel oil and 12 parts oat bran extract.

[0060] Everything else is the same as in Example 1.

[0061] Example 3

[0062] Unlike Example 1, the weight ratio of oat kernel oil and oat bran extract in the oat refined oil extract is different.

[0063] An oat refined oil extract comprises, by weight, 92 parts oat kernel oil and 8 parts oat bran extract.

[0064] Everything else is the same as in Example 1.

[0065] Example 4

[0066] An oat refined oil extract comprises, by weight, 90 parts oat kernel oil and 10 parts oat bran extract.

[0067] The preparation method of oat bran extract is as follows:

[0068] (1) Mix oat bran with a mixed solution of ethanol-n-hexane-limonene in a volume ratio of 6:2:1. The solid-liquid ratio after mixing is 1:20 g / mL. Perform intermittent microwave treatment at 200W power for 5 min (intermittent once every 1 min). Filter to obtain extract solution A and oat bran residue.

[0069] (2) Oat bran residue was subjected to supercritical carbon dioxide extraction with a mixed solution of ethanol-n-hexane-limonene in a volume ratio of 6:2:1. The extraction temperature was 45℃, the extraction pressure was 60MPa, and the static extraction time was 50min to obtain extraction solution B.

[0070] (3) Mix extraction solution A and extraction solution B, concentrate under reduced pressure to separate the extract, and obtain oat bran extract.

[0071] The preparation method of refined oat oil extract is as follows:

[0072] S1. Preparation of oat kernel oil: Oat kernels were pressed at 35℃ and 70MPa for 40 minutes, repeated twice, and filtered to obtain oat kernel oil A and oat residue.

[0073] Oat residue was subjected to supercritical carbon dioxide extraction at an extraction pressure of 25 MPa, an extraction temperature of 26 °C, an entrainer of ethanol (300 mL / 100 g), and a dynamic extraction time of 20 min to obtain oat kernel oil B.

[0074] Oat kernel oil A and oat kernel oil B are combined into oat kernel oil;

[0075] S2. Add oat kernel oil to an appropriate amount of deionized water at the same temperature, centrifuge to separate the oil layer; add activated carbon for decolorization; add the decolorized product to an alkaline solution with pH=8, stir, heat to 55℃, let stand and cool to room temperature, refrigerate at 4℃ for 12 hours, centrifuge, and collect the deacidified product; heat the deacidified product to 85℃ and wash with water until neutral, then separate the oat kernel oil after washing.

[0076] Oat kernel oil and oat bran extract are mixed to obtain refined oat oil extract.

[0077] Example 5

[0078] An oat refined oil extract comprises, by weight, 90 parts oat kernel oil and 10 parts oat bran extract.

[0079] The preparation method of oat bran extract is as follows:

[0080] (1) Mix oat bran with a mixed solution of ethanol-n-hexane-limonene in a volume ratio of 10:4:2. The solid-liquid ratio after mixing is 1:20 g / mL. Perform intermittent microwave treatment at 400W power for 2 min (intermittent once every 1 min). Filter to obtain extract solution A and oat bran residue.

[0081] (2) Oat bran residue was subjected to supercritical carbon dioxide extraction with a mixed solution of ethanol-n-hexane-limonene in a volume ratio of 10:4:2. The extraction temperature was 35℃, the extraction pressure was 50MPa, and the static extraction time was 80min to obtain extraction solution B.

[0082] (3) Mix extraction solution A and extraction solution B, concentrate under reduced pressure to separate the extract, and obtain oat bran extract.

[0083] The preparation method of refined oat oil extract is as follows:

[0084] S1. Preparation of oat kernel oil: Oat kernels were pressed at 45℃ and 50MPa for 20 minutes, repeated twice, and filtered to obtain oat kernel oil A and oat residue.

[0085] Oat residue was subjected to supercritical carbon dioxide extraction at an extraction pressure of 30 MPa, an extraction temperature of 30 °C, an entrainer of ethanol (200 mL / 100 g), and a dynamic extraction time of 10 min to obtain oat kernel oil B.

[0086] Oat kernel oil A and oat kernel oil B are combined into oat kernel oil;

[0087] S2. Add oat kernel oil to an appropriate amount of deionized water at the same temperature, centrifuge to separate the oil layer; add activated carbon for decolorization; add the decolorized product to an alkaline solution with pH=8, stir, heat to 55℃, let stand and cool to room temperature, refrigerate at 4℃ for 12 hours, centrifuge, and collect the deacidified product; heat the deacidified product to 85℃ and wash with water until neutral, then separate the oat kernel oil after washing.

[0088] Oat kernel oil and oat bran extract are mixed to obtain refined oat oil extract.

[0089] Comparative Example 1

[0090] Unlike Example 1, the weight ratio of oat kernel oil and oat bran extract in the oat refined oil extract is different.

[0091] The refined oat oil extract comprises, by weight, 98 parts oat kernel oil and 2 parts oat bran extract.

[0092] Everything else is the same as in Example 1.

[0093] Comparative Example 2

[0094] Unlike Example 1, the preparation method of the refined oat oil extract is different; this comparative example does not separate the extraction of oat kernel oil and oat bran extract. Specifically:

[0095] Oat seeds containing oat kernels and oat bran were pressed at 40℃ and 62MPa for 30 min, repeated twice, and filtered to obtain oat oil A and oat residue. The oat residue was subjected to supercritical carbon dioxide extraction at an extraction pressure of 28MPa, an extraction temperature of 28℃, an entrainer of ethanol (250mL / 100g), and a dynamic extraction time of 15 min to obtain oat oil B. Oat oil A and oat kernel B were combined to form oat oil.

[0096] Add oat oil to an appropriate amount of deionized water at the same temperature, centrifuge to separate the oil layer; add activated carbon for decolorization; add the decolorized product to an alkaline solution with pH=8, stir, heat to 55℃, let stand and cool to room temperature, refrigerate at 4℃ for 12 hours, centrifuge, and collect the deacidified product; heat the deacidified product to 85℃ and wash with water until neutral, then separate to obtain refined oat oil extract.

[0097] Comparative Example 3

[0098] Unlike Example 1, in the preparation method of oat bran extract, limonene in steps (1) and (2) is replaced with acetone. All other steps are the same as in Example 1.

[0099] Comparative Example 4

[0100] Unlike Example 1, in the preparation method of oat bran extract, the volume ratio of ethanol-n-hexane-limonene in steps (1) and (2) is different, both being 2:3:8.

[0101] Everything else is the same as in Example 1.

[0102] Comparative Example 5

[0103] Unlike Example 1, the method for preparing the refined oat oil extract is different, specifically the method for preparing the oat kernel oil is different:

[0104] S1. Preparation of oat kernel oil: Oat kernels were pressed at 90℃ and 120MPa for 30 minutes, repeated twice, and filtered to obtain oat kernel oil A and oat residue;

[0105] Oat residue was subjected to supercritical carbon dioxide extraction at an extraction pressure of 28 MPa, an extraction temperature of 28 °C, an entrainer of ethanol (250 mL / 100 g), and a dynamic extraction time of 15 min to obtain oat kernel oil B.

[0106] Oat kernel oil A and oat kernel oil B are combined into oat kernel oil;

[0107] S2, Same as Example 1 (the preparation method of oat bran extract is the same as in Example 1).

[0108] Everything else is the same as in Example 1.

[0109] Experiment 1: Extraction yield of oat bran extract

[0110] The extraction yield is calculated using the following formula:

[0111] Extraction yield (%) of oat bran extract = m A / m B ×100%;

[0112] Where, m A Mass of oat bran extract (g); m B The quality of oat bran.

[0113] The extraction yields of oat bran extracts obtained in Examples 1, 4-5, and 3-4 were calculated, and the results are shown in Table 1.

[0114] Table 1

[0115]

[0116] In Table 1, # Indicates a significant difference. # P < 0.05 indicates that the extraction yield of the comparative example is significantly different from that of Example 1.

[0117] As can be seen from Table 1, the preparation method of the present invention, compared with comparative examples 3-4 which changed the type and ratio of extraction solvent, brings about a significant improvement in extraction yield.

[0118] Experiment 2 Transparency Detection

[0119] The transparency of the oat refined oil extracts prepared in Examples 1-5, Comparative Example 2, and Comparative Example 5 was tested.

[0120] The identification of transparency and color of vegetable oils was performed using GB / T 5525-2008.

[0121] After testing, it was found that the oat oil extracts of Examples 1-5 were all transparent light yellow; the oat oil extract of Comparative Example 2 was slightly turbid; and the oat oil extract of Comparative Example 5 was slightly turbid brownish-yellow.

[0122] This indicates that when the components of the oat oil extract are mixed or the conditions such as the temperature and pressure of oat kernel pressing are changed, the appearance and properties of the oat oil extract deteriorate.

[0123] Experiment 3 Moisturizing performance test

[0124] Test samples: various refined oat oil extracts. Blank group sample: water.

[0125] Volunteers: 50 healthy women aged 25-40 years, without skin diseases, who had not undergone other efficacy clinical trials in the past 3 months, and who voluntarily participated and signed informed consent forms. They were randomly divided into 10 groups of 5 people each, with no significant difference in initial mean TWEL between groups.

[0126] Test indicator: TWEL value.

[0127] Testing Method: First, three 3×3cm square test areas were marked on the inner side of each volunteer's arm, resulting in six test areas per volunteer's arm. The initial TEWL value of each test area was measured. Then, 3M tape was repeatedly peeled and torn 10 times in each test area to construct a skin barrier damage model. 0.2g of test sample was then applied to each area, and a blank control group (water) was set up. After the volunteers sat still for 30 minutes, the TEWL value of each test area was measured. The average TEWL value of each test group was calculated. The average TEWL results are shown in Table 2.

[0128] Table 2

[0129]

[0130] In Table 2, # , ## Indicates a significant difference. # P < 0.05 indicates that the extraction yield of the comparative example is significantly different from that of Example 1; ## P < 0.01 indicates that the extraction yield of the comparative example is significantly different from that of Example 1.

[0131] As shown in Table 2, the refined oat oil extract of the present invention can effectively reduce the TEWL value of the skin barrier damage model, exhibiting excellent repair and moisturizing effects. Comparing Examples 1-5 with Comparative Examples 1-2, it is evident that oat kernel oil and oat bran extract, prepared and mixed in specific ratios, significantly enhance moisturizing and repairing effects within a certain ratio range. Comparing Examples 1-5 with Comparative Examples 3-4, it is evident that the extraction solvent components used in the oat bran extract mutually promote each other, enhancing the expression of active ingredients in the extract and maintaining high repair and moisturizing effects. Comparing Examples 1-5 with Comparative Example 5, it is evident that oat kernel oil, under relatively low-temperature pressing conditions, can retain the maximum amount of active ingredients, has low impurity content, and exhibits excellent repair and moisturizing effects.

[0132] Experiment 4 Anti-inflammatory properties

[0133] Inflamed skin, due to external stimuli, induces epidermal keratinocytes to release inflammation-related factors, such as the inflammatory cytokines IL-1β (interleukin-1β) and TNF-α (tumor necrosis factor), promoting an increase in the concentrations of cytokines IL-6 (interleukin-6) and TGF-α (transforming growth factor-α). The anti-inflammatory effect of the oat refined oil extract of this invention was detected using the concentrations of TNF-α and IL-6 as indicators.

[0134] HaCaT cells were cultured in DMEM cell culture medium at 37°C and CO2. The culture medium was replaced after 48 hours until the cell confluence reached more than 80%. Then, the cells were divided into the following groups:

[0135] Example 1-Example 5, Comparative Example 1-Comparative Example 5: treated with 1 μg / mL LPS for 24 h, then replaced with culture medium containing 100 μg / mL oat refined oil extract and cultured for another 24 h.

[0136] Control group: Cells were cultured normally;

[0137] LPS model group: also known as the model damage group, treated with 1 μg / mL LPS for 24 h, then replaced with ordinary culture medium and cultured for another 24 h.

[0138] Cells were sequentially treated with LPS and then with samples. The supernatant was collected, and the concentration of pro-inflammatory factors in the supernatant was detected using an ELISA kit. This experiment used an ELISA kit for TNF-α and IL-6, purchased from Shanghai Enzyme-Linked Biotechnology Co., Ltd. The concentrations of TNF-α and IL-6 were detected according to the kit's instructions. The steps included: diluting the cell supernatant to the detection range of the ELISA kit, then adding it to antibody-coated 96-well plates for incubation; adding the antibody for the target analyte and the chromogenic reagent TMB; adding the reaction stop solution; and then performing the detection using a microplate reader. A standard curve was plotted based on the standards provided in the kit, and the concentration of pro-inflammatory factors in the supernatant was calculated.

[0139] The concentrations of TNF-α and IL-6 in each group are shown in Table 3.

[0140] Table 3

[0141]

[0142] In Table 3, # , ## Indicates a significant difference. # P < 0.05 indicates that the extraction yield of the comparative example is significantly different from that of Example 1; ## P < 0.01 indicates that the extraction yield of the comparative example is significantly different from that of Example 1.

[0143] As can be seen from Table 3, the refined oat oil extract of the present invention has excellent inhibitory effects on the expression of pro-inflammatory factors TNF-α and IL-6; compared with the comparative group, the refined oat oil extract of the example group has significantly improved anti-inflammatory effect.

[0144] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. An oat oil extract, characterized in that, It is made from the following ingredients in parts by weight: 88-92 parts oat kernel oil and 8-12 parts oat bran extract; The preparation method of the oat bran extract includes the following steps: (1) Mix oat bran with a mixed solution of ethanol-n-hexane-limonene and microwave at 200-400W for 2-5 minutes to obtain extract solution A and oat bran residue; (2) Supercritical carbon dioxide extraction was performed on oat bran residue, with a mixed solution of ethanol-n-hexane-limonene as the entrainer. The extraction temperature was 35-45℃, the extraction pressure was 50-60MPa, and the static extraction time was 50-80min to obtain extraction solution B. (3) Mix extraction solution A and extraction solution B, concentrate under reduced pressure to separate the extract, and obtain oat bran extract; In the mixed solution described in steps (1) and (2), the volume ratio of ethanol, n-hexane and limonene is 6-10:2-4:1-2; The method for preparing oat kernel oil includes the following steps: pressing oat kernels at 35-45℃ and 50-70MPa for 20-40 minutes, repeating the pressing 1-3 times to obtain oat kernel oil A and oat residue; subjecting the oat residue to supercritical carbon dioxide extraction at an extraction pressure of 25-30MPa, an extraction temperature of 26-30℃, using ethanol as the entrainer at a dosage of 200-300mL / 100g, and a dynamic extraction time of 10-20 minutes to obtain oat kernel oil B; combining oat kernel oil A and oat kernel oil B, adding an appropriate amount of deionized water, and centrifuging to separate the oil layer; adding activated carbon for decolorization; adding the decolorized product to an alkaline solution, stirring, heating to 55℃, allowing it to cool to room temperature, refrigerating at 4℃ for 12 hours, centrifuging, and collecting the deacidified product; heating the deacidified product to 85℃, washing with water until neutral, and then separating the oat kernel oil.

2. The oat oil extract according to claim 1, characterized in that, The mass ratio of oat kernel oil to oat bran extract is 90:

10.

3. The oat oil extract according to claim 1, characterized in that, In the mixed solution described in steps (1) and (2), the volume ratio of ethanol, n-hexane and limonene is 8:3:

2.

4. The oat oil extract according to claim 1, characterized in that, The microwave treatment in step (1) is performed under the following conditions: 300W microwave power for 3 minutes; the solid-liquid ratio of the microwave treatment is 1:16-25g / mL.

5. The oat oil extract according to claim 1, characterized in that, The conditions for supercritical carbon dioxide extraction in step (2) are: extraction temperature 40℃, extraction pressure 55MPa, and static extraction time 60min.

6. A method for preparing the oat refined oil extract according to any one of claims 1-5, characterized in that, Including the following steps: S1. Preparation of oat kernel oil: Oat kernels are pressed at 35-45℃ and 50-70MPa for 20-40 minutes, and the pressing is repeated 1-3 times to obtain oat kernel oil A and oat residue; the oat residue is subjected to supercritical carbon dioxide extraction at an extraction pressure of 25-30MPa and an extraction temperature of 26-30℃, with ethanol as the entrainer at a dosage of 200-300mL / 100g, and a dynamic extraction time of 10-20 minutes to obtain oat kernel oil B; oat kernel oil A and oat kernel oil B are combined, an appropriate amount of deionized water is added, and the oil layer is separated by centrifugation; activated carbon is added for decolorization; the decolorized product is added to alkaline solution, stirred, heated to 55℃, allowed to stand and cool to room temperature, refrigerated at 4℃ for 12 hours, centrifuged, and the deacidified product is collected; the deacidified product is heated to 85℃, washed with water until neutral, and the oat kernel oil is separated by water washing. S2. Mix oat kernel oil and oat bran extract to obtain refined oat oil extract.

7. The use of the oat refined oil extract according to any one of claims 1-5 or the oat refined oil extract prepared by the preparation method according to claim 6 in the preparation of moisturizing and anti-inflammatory cosmetics.

8. A moisturizing and anti-inflammatory cosmetic, characterized in that, The components contain the oat refined oil extract according to any one of claims 1-5 or the oat refined oil extract prepared by the preparation method according to claim 6.

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