Traditional Chinese medicine composition for treating cholecystitis

Abstract

The invention provides a traditional Chinese medicine composition for treating cholecystitis. The traditional Chinese medicine composition is prepared from the following raw material medicines: desmodium styracifolium, lygodium japonicum, endothelium corneum gigeriae galli, radix curcumae, pinellia ternate, scutellaria baicalensis, coptis chinensis, radix paeoniae alba and fructus aurantii. Animal experiment research results show that the traditional Chinese medicine composition has an obvious treatment effect on guinea pig cholecystitis caused by lincomycin hydrochloride, can obviously reduce guinea pig bile volume and reduce guinea pig serum TNF-alpha and IL-1beta, and has a recovery effect on liver function damage.

Description

Technical Field

[0001] The present application relates to a traditional Chinese medicine composition for treating cholecystitis and its preparation and application, belonging to the technical field of traditional Chinese medicine. Background Art

[0002] Chronic cholecystitis is a common digestive system disease characterized by symptoms such as right upper abdominal pain, belching, aversion to greasy foods, decreased appetite, nausea and vomiting, and fever. The disease can last for more than ten years. Due to its high recurrence rate and long course, chronic cholecystitis has a significant impact on patients. Chronic cholecystitis is primarily caused by the long-term presence of gallstones or recurrent acute cholecystitis. A variety of other causes can also contribute to recurrence, including abnormal bile composition and biliary tract motility disorders.

[0003] Clinically, Western medicine treatments for chronic cholecystitis primarily rely on surgery and medications, which offer promising short-term results but poor long-term outcomes, with a high risk of recurrence after discontinuation. However, Traditional Chinese Medicine (TCM) is often widely used in patients with clear clinical symptoms. Following the principle of combining "monarch, minister, adjuvant, and guiding" therapies, it regulates the functional system holistically, offering multi-target, multi-system advantages in the prevention and treatment of chronic calculous cholecystitis. Summary of the Invention

[0004] In order to solve the problems existing in the prior art, the present invention provides a traditional Chinese medicine composition for treating cholecystitis and a preparation method thereof, as well as application of the traditional Chinese medicine composition in preparing a medicament for treating cholecystitis.

[0005] As one aspect of the present invention, the present invention provides a traditional Chinese medicine composition for treating cholecystitis, which is prepared from Herba Lycopodii, Lygodium japonicum, Gallus gallifolia, Curcuma aromatica, Pinellia ternata, Scutellaria baicalensis, Coptis chinensis, Paeonia lactiflora, and Fructus aurantii.

[0006] Preferably, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 6-30 parts by weight of Herba Lygodii, 5-25 parts by weight of Lygodium japonicum, 5-25 parts by weight of Gallus galli, 5-25 parts by weight of Curcuma aromatica, 3-15 parts by weight of Pinellia ternata, 3-15 parts by weight of Scutellaria baicalensis, 2-10 parts by weight of Coptis chinensis, 5-25 parts by weight of White Peony Root, and 5-25 parts by weight of Fructus Aurantii Immaturus.

[0007] Further preferably, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 7-22 parts by weight of Desmodium sibiricum, 6-18 parts by weight of Lygodium japonicum, 6-18 parts by weight of Gallus gallus domesticus, 6-18 parts by weight of Curcuma aromatica, 4-12 parts by weight of Pinellia ternata, 4-12 parts by weight of Scutellaria baicalensis, 2.5-7.5 parts by weight of Coptis chinensis, 6-18 parts by weight of White Peony Root, and 6-18 parts by weight of Fructus Aurantii Immaturus.

[0008] More preferably, the traditional Chinese medicine composition is made of the following raw materials in parts by weight: 8-15 parts by weight of Herba Lygodii, 7-12 parts by weight of Lygodium japonicum, 7-12 parts by weight of Gallus galli, 7-12 parts by weight of Curcuma aromatica, 5-8 parts by weight of Pinellia ternata, 5-8 parts by weight of Scutellaria baicalensis, 3-4 parts by weight of Coptis chinensis, 7-12 parts by weight of White Peony Root, and 7-12 parts by weight of Fructus Aurantii Immaturus.

[0009] Most preferably, the Chinese medicine composition is made of the following raw materials in parts by weight: 10 parts by weight of Desmodium strychnine, 8 parts by weight of Lygodium japonicum, 8 parts by weight of Chicken's Gizzard Stone, 8 parts by weight of Curcuma aromatica, 6 parts by weight of Pinellia ternata, 6 parts by weight of Scutellaria baicalensis, 4 parts by weight of Coptis chinensis, 8 parts by weight of White Peony Root, and 8 parts by weight of Fructus Aurantii Immaturus.

[0010] In the above technical solution, the Chinese medicine composition is a composition formed by mixing the raw materials after crushing, or an extract obtained by mixing or extracting the raw materials separately, or an effective part obtained by further refining and purifying the extract, or a conventional dosage form prepared by adding pharmaceutically acceptable excipients to the extract / effective part.

[0011] The above-mentioned extraction methods include decoction extraction, reflux extraction, maceration extraction, ultrasonic extraction, percolation extraction, microwave extraction, etc.; the extraction solvent includes water or conventional organic solvents, such as one or more of methanol, ethanol, acetone, ethyl acetate, petroleum ether, and isopropanol; the refining and purification methods include extraction, water extraction and alcohol precipitation, alkali dissolution and acid precipitation, and various column chromatography purification methods, such as macroporous resin column, silica gel column, gel column, reverse phase column, etc.

[0012] The conventional dosage forms mentioned above include, but are not limited to, injections, capsules, tablets, granules, gels, sustained-release formulations, oral solutions, pills, or nanoformulations; pharmaceutically acceptable excipients include fillers, disintegrants, lubricants, suspending agents, binders, sweeteners, flavoring agents, preservatives, and bases. Fillers include starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, and sucrose; disintegrants include starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, cross-linked polyvinyl pyrrolidone, low-substituted hydroxypropyl cellulose, and cross-linked sodium carboxymethyl cellulose; lubricants include magnesium stearate, sodium lauryl sulfate, talc, and silicon dioxide; suspending agents include polyvinyl pyrrolidone, microcrystalline cellulose, sucrose, agar, and hydroxypropyl methylcellulose; and binders include starch slurry, polyvinyl pyrrolidone, and hydroxypropyl methylcellulose.

[0013] As another aspect of the present invention, the Chinese medicine composition of the present invention can also be made from the extracts of the raw materials. Therefore, the present invention further provides a Chinese medicine composition for treating cholecystitis, which is made from the following raw materials: 6-30 parts by weight of Desmodium sibiricum extract, 5-25 parts by weight of Lygodium japonicum extract, 5-25 parts by weight of Gallus gallifolia extract, 5-25 parts by weight of Curcuma aromatica extract, 3-15 parts by weight of Pinellia ternata extract, 3-15 parts by weight of Scutellaria baicalensis extract, 2-10 parts by weight of Coptis chinensis extract, 5-25 parts by weight of White Peony Root extract, and 5-25 parts by weight of Fructus Aurantii Immaturus extract.

[0014] Further preferably, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 7-22 parts by weight of Desmodium sibiricum extract, 6-18 parts by weight of Lygodium japonicum extract, 6-18 parts by weight of Gallus gallifolia extract, 6-18 parts by weight of Curcuma aromatica extract, 4-12 parts by weight of Pinellia ternata extract, 4-12 parts by weight of Scutellaria baicalensis extract, 2.5-7.5 parts by weight of Coptis chinensis extract, 6-18 parts by weight of White Peony Root extract, and 6-18 parts by weight of Fructus Aurantii Immaturus extract;

[0015] More preferably, the traditional Chinese medicine composition is made from the following raw materials in parts by weight: 8-15 parts by weight of Desmodium sibiricum extract, 7-12 parts by weight of Lygodium japonicum extract, 7-12 parts by weight of Gallus gallifolia extract, 7-12 parts by weight of Curcuma aromatica extract, 5-8 parts by weight of Pinellia ternata extract, 5-8 parts by weight of Scutellaria baicalensis extract, 3-4 parts by weight of Coptis chinensis extract, 7-12 parts by weight of White Peony Root extract, and 7-12 parts by weight of Fructus Aurantii Immaturus extract.

[0016] Most preferably, the Chinese medicine composition is made from the following raw materials in parts by weight: 10 parts by weight of Desmodium sibiricum extract, 8 parts by weight of Lygodium japonicum extract, 8 parts by weight of Gallus gallifolia extract, 8 parts by weight of Curcuma aromatica extract, 6 parts by weight of Pinellia ternata extract, 6 parts by weight of Scutellaria baicalensis extract, 4 parts by weight of Coptis chinensis extract, 8 parts by weight of White Peony Root extract, and 8 parts by weight of Fructus Aurantii Immaturus extract.

[0017] The above extracts can be water extracts of the respective APIs, or organic solvent extracts, or refined products obtained by further refining and purification of the water extracts / organic solvent extracts.

[0018] The organic solvent is selected from methanol, ethanol, acetone, ethyl acetate, petroleum ether, and isopropanol;

[0019] The extraction method used to prepare the above extract includes any one of decoction extraction, reflux extraction, maceration extraction, ultrasonic extraction, percolation extraction, microwave extraction, or a combination of different extraction methods.

[0020] As another aspect of the present invention, the present invention provides a method for preparing the Chinese medicine composition, the method comprising the following steps:

[0021] Step 1: Take the raw material medicine in proportion;

[0022] Step 2: extracting the raw material drug with a solvent to obtain an extract;

[0023] Step three, preparing the extract into injection, capsule, tablet, granule, gel, sustained-release preparation, oral solution, pill or nano preparation according to conventional preparation process.

[0024] The solvent includes one or more of water, methanol, ethanol, acetone, ethyl acetate, petroleum ether, and isopropanol; and the extraction method includes decoction extraction, reflux extraction, immersion extraction, ultrasonic extraction, percolation extraction, and microwave extraction.

[0025] As another aspect of the present invention, the present invention also provides use of the traditional Chinese medicine composition in preparing a medicine for treating cholecystitis.

[0026] The raw materials used in the present invention are all included in the Chinese Pharmacopoeia (2020 edition). Among them, Pinellia ternata is preferably Pinellia ternata.

[0027] Beneficial effects of the Chinese medicine composition of the present invention:

[0028] In this prescription, the herb Centella asiatica is sweet, salty and slightly cold, and enters the liver, gallbladder, kidney and bladder meridians; the herb Sea Sand is sweet, salty and cold, and enters the bladder and small intestine meridians; the herb Chicken Gizzard is sweet and neutral, and enters the spleen, stomach, small intestine and bladder meridians. The three are collectively the monarch medicines, and their sweetness relieves urgency, saltiness softens hard masses, and coldness clears heat, and together they have the effect of clearing heat, promoting dampness and relieving pain; the herb Curcuma aromatica is pungent and cold, and enters the liver, heart and lung meridians, and is pungent to disperse it, bitter and cold to dry dampness and clear heat, and to soothe the liver, relieve depression and promote bile secretion, and assist the monarch medicine in strengthening the effect of clearing heat and promoting dampness, and is the minister medicine; the herb Pinellia is pungent and warm in nature, and enters the spleen, stomach and lung meridians, and can dry dampness and resolve phlegm, reduce adverse reactions and stop vomiting; the herb Scutellaria baicalensis and the herb Coptis chinensis are both bitter and cold in nature, and enter the gallbladder, stomach and large intestine meridians, and can clear heat, dry dampness and purge fire The three herbs act as adjuvants to detoxify, enhancing the effects of clearing heat and drying dampness, and relieving symptoms such as nausea and vomiting caused by cholecystitis. Meanwhile, Scutellaria baicalensis and Coptis chinensis can counteract the pungent and warm nature of Pinellia ternata. White Peony Root is bitter, sour, and slightly cold in nature, and enters the Liver and Spleen Meridians. It has the effects of nourishing blood, restraining Yin, softening the liver, and relieving pain. As an adjuvant in the prescription, it can relieve pain and balance the potent effects of other herbs. Fructus Aurantii Immaturus is bitter, acrid, and sour in nature, and slightly cold in nature, and enters the Spleen and Stomach Meridians. As an adjuvant, it can regulate Qi, relieve fullness, and promote Qi circulation and eliminate stagnation. It has an effect on improving symptoms such as abdominal distension and belching caused by cholecystitis, while guiding the herbs in the prescription directly to the affected area, enhancing the therapeutic effect of the entire prescription. This prescription treats symptoms related to cholecystitis by clearing heat and dampness, drying dampness and promoting bile secretion, nourishing Yin and softening the liver, relieving pain, harmonizing the stomach and relieving adverse reactions, and regulating Qi and relieving stagnation.

[0029] The prescription of the present invention is characterized by the addition of scutellaria baicalensis, coptis chinensis and pinellia ternata. Scutellaria baicalensis and coptis chinensis can firstly clear damp-heat in the upper and middle burners, assist the monarch and ministerial herbs in clearing away heat and dampness, secondly restrain the pungent and warm nature of pinellia ternata, and thirdly protect the liver and promote bile secretion, thereby alleviating the symptoms of damp-heat in cholecystitis. Pinellia ternata and ginger pinellia ternata are commonly used processed products of pinellia ternata, both of which can dry out dampness and resolve phlegm, and relieve nausea and vomiting. However, the function of drying dampness in pinellia ternata is even greater, and although ginger pinellia ternata is slightly less toxic than that of scutellaria baicalensis, its nature is warm and dry. Therefore, for the symptoms of damp-heat accumulation in cholecystitis, the selection of scutellaria ternata in this prescription is more symptomatic.

[0030] The results of animal experiments show that the Chinese medicine composition of the present invention has a significant therapeutic effect on lincomycin hydrochloride-induced cholecystitis in guinea pigs. It can significantly reduce the bile volume of guinea pigs, reduce TNF-α and IL-1β in the serum of guinea pigs, and has a restorative effect on liver function damage. Its therapeutic effect is equivalent to that of the positive drug anti-inflammatory and choleretic tablets. DETAILED DESCRIPTION

[0031] The present invention is further described below by way of specific examples, but the present invention is not limited to the following examples. Within the scope of the present invention or without departing from the content, spirit and scope of the present invention, changes, combinations or substitutions of the present invention will be obvious to those skilled in the art and are included within the scope of the present invention.

[0032] Example 1

[0033] Prescription: Herba Lysimachiae 10g, Herba Lysimachiae 8g, Herba Galli 8g, Herba Curcumae 8g, Herba Pinelliae 6g, Herba Scutellariae 6g, Herba Coptis 4g, Herba Paeoniae Alba 8g, Fructus Aurantii Immaturus 8g.

[0034] Preparation method: Add water to the above 9 ingredients and boil them three times, 12 times the amount of water each time, boil for 1 hour, filter, and concentrate the filtrate under reduced pressure at 60-80℃ until the relative density reaches 1.02-1.11 (60℃). Dry under reduced pressure at 60-70℃ and grind to obtain extract powder. Add appropriate amount of dextrin (drug-excipient ratio 2:1-1:1), mix well, and make into granules.

[0035] Example 2

[0036] Prescription: Herba Lysimachiae 8.5g, Herba Lycopodii 9g, Gallus domestica 7.5g, Curcuma 9g, Herba Pinelliae 5.5g, Herba Scutellariae 7g, Herba Coptis 3.5g, Herba Paeoniae Alba 9g, Fructus Aurantii Immaturus 7.5g.

[0037] The raw materials are taken in proportion, crushed, and prepared into capsules according to conventional preparation technology.

[0038] Example 3

[0039] Prescription: Herba Lysimachiae 12g, Herba Lycopodii 7.5g, Gallus domestica 9g, Curcuma aromatica 7.5g, Herba Pinelliae 7g, Herba Scutellariae 5.5g, Herba Coptis 6g, Herba Paeoniae Alba 7.5g, Fructus Aurantii Immaturus 9g.

[0040] The raw materials are taken in proportion, crushed, and made into tablets according to conventional preparation technology.

[0041] Example 4

[0042] Prescription: Herba Lysimachiae 8g, Herba Lycopodii 11g, Gallus galli 7g, Curcuma aromatica 11g, Rhizoma Pinelliae 5g, Radix Scutellariae 8g, Rhizoma Coptidis 3g, Radix Paeoniae Alba 11g, Fructus Aurantii Immaturus 7g.

[0043] The raw materials are taken in proportion, crushed, and made into pills according to conventional preparation technology.

[0044] Example 5

[0045] Prescription: Herba Lysimachiae 14g, Herba Lycopodii 7g, Gallus galli 11g, Curcuma aromatica 7g, Herba Pinelliae 8g, Herba Scutellariae 5g, Herba Coptis 7g, Herba Paeoniae Alba 7g, Fructus Aurantii Immaturus 11g.

[0046] The raw materials are taken in proportion, decocted in water for extraction, and the decoction is made into oral liquid according to conventional preparation technology.

[0047] Example 6

[0048] Prescription: Herba Lysimachiae 7.5g, Herba Lycopodii 14g, Herba Galli 6.5g, Herba Curcumae 14g, Herba Pinelliae 4.5g, Herba Scutellariae 9g, Herba Coptis 3g, Herba Paeoniae Alba 14g, Fructus Aurantii Immaturus 6.5g.

[0049] The raw materials are taken in proportion, decocted in water for extraction, and the decoction is made into granules according to conventional preparation technology.

[0050] Example 7

[0051] Prescription: Herba Lysimachiae 18g, Herba Lycopodii 6.5g, Gallus domestica 14g, Curcuma aromatica 6.5g, Herba Pinelliae 9g, Herba Scutellariae 4.5g, Herba Coptis 7g, Herba Paeoniae Alba 6.5g, Fructus Aurantii Immaturus 14g.

[0052] The raw materials are taken in proportion, added with 75% ethanol for reflux extraction, and the extract is made into capsules according to conventional preparation technology.

[0053] Example 8

[0054] Prescription: Herba Lysimachiae 7g, Herba Lycopodii 16g, Gallus galli 6g, Curcuma aromatica 16g, Herba Pinelliae 4g, Herba Scutellariae 11g, Herba Coptis 2.5g, Herba Paeoniae Alba 16g, Fructus Aurantii Immaturus 6g.

[0055] The raw materials are taken in proportion, added with 75% ethanol for reflux extraction, and the extract is made into tablets according to conventional preparation technology.

[0056] Example 9

[0057] Prescription: Herba Lysimachiae 20g, Herba Lycopodii 6g, Gallus galli 16g, Curcuma aromatica 6g, Herba Pinelliae 11g, Herba Scutellariae 4g, Herba Coptis 7.5g, Herba Paeoniae Alba 6g, Fructus Aurantii Immaturus 16g.

[0058] The raw materials are taken in proportion, 75% ethanol is added for reflux extraction, and the extract is made into pills according to a conventional preparation process.

[0059] Example 10

[0060] Prescription: Herba Lysimachiae 6.5g, Herba Lycopodii 20g, Herba Galli 5.5g, Herba Curcumae 20g, Herba Pinelliae 3.5g, Herba Scutellariae 13g, Herba Coptis 2.5g, Herba Paeoniae Alba 20g, Fructus Aurantii Immaturus 5.5g.

[0061] Example 11

[0062] Prescription: Herba Lysimachiae 24g, Herba Lycopodii 5.5g, Gallus domestica 20g, Curcuma 5.5g, Herba Pinelliae 13g, Herba Scutellariae 3.5g, Herba Coptis 8g, Herba Paeoniae Alba 5.5g, Fructus Aurantii Immaturus 20g.

[0063] Example 12

[0064] Prescription: Herba Lysimachiae 6g, Herba Lycopodii 23g, Gallus galli 5g, Curcuma 23g, Herba Pinelliae 3g, Herba Scutellariae 14g, Herba Coptis 2g, Herba Paeoniae Alba 23g, Fructus Aurantii Immaturus 5g.

[0065] Example 13

[0066] Prescription: Herba Lysimachiae 27g, Herba Lycopodii 5g, Gallus domestica 23g, Curcuma 5g, Herba Pinelliae 14g, Herba Scutellariae 3g, Herba Coptis 9g, Herba Paeoniae Alba 5g, Fructus Aurantii Immaturus 23g.

[0067] Example 14

[0068] Prescription: 10g of Desmodium sibiricum extract, 8g of Lycopodiella chinensis extract, 8g of Gallus gallus domesticus extract, 8g of Curcuma aromatica extract, 6g of Pinellia ternata extract, 6g of Scutellaria baicalensis extract, 4g of Coptis chinensis extract, 8g of White Peony Root extract, and 8g of Citrus aurantium extract.

[0069] Example 15

[0070] Formula: 8.5g of Desmodium sabdariffa extract, 9g of Lycopodiella chinensis extract, 7.5g of Gallus gallus domesticus extract, 9g of Curcuma aromatica extract, 5.5g of Pinellia ternata extract, 7g of Scutellaria baicalensis extract, 3.5g of Coptis chinensis extract, 9g of White Peony Root extract, and 7.5g of Citrus aurantium extract.

[0071] Example 16

[0072] Formula: 12g of Desmodium sibiricum extract, 7.5g of Lycopodiella chinensis extract, 9g of Gallus gallus domesticus extract, 7.5g of Curcuma aromatica extract, 7g of Pinellia ternata extract, 5.5g of Scutellaria baicalensis extract, 6g of Coptis chinensis extract, 7.5g of White Peony Root extract, and 9g of Citrus aurantium extract.

[0073] Example 17

[0074] Formula: 8g of Desmodium sibiricum extract, 11g of Lycopodiella chinensis extract, 7g of Gallus gallus domesticus extract, 11g of Curcuma aromatica extract, 5g of Pinellia ternata extract, 8g of Scutellaria baicalensis extract, 3g of Coptis chinensis extract, 11g of White Peony Root extract, and 7g of Citrus aurantium extract.

[0075] Example 18

[0076] Formula: 14g of Desmodium sibiricum extract, 7g of Lycopodiella chinensis extract, 11g of Gallus gallus domesticus extract, 7g of Curcuma aromatica extract, 8g of Pinellia ternata extract, 5g of Scutellaria baicalensis extract, 7g of Coptis chinensis extract, 7g of White Peony Root extract, and 11g of Citrus aurantium extract.

[0077] Example 19

[0078] Formula: 7.5g of Desmodium sabdariffa extract, 14g of Lycopodiella chinensis extract, 6.5g of Gallus gallus domesticus extract, 14g of Curcuma aromatica extract, 4.5g of Pinellia ternata extract, 9g of Scutellaria baicalensis extract, 3g of Coptis chinensis extract, 14g of White Peony Root extract, and 6.5g of Citrus aurantium extract.

[0079] Example 20

[0080] Formula: 18g of Desmodium sabdariffa extract, 6.5g of Lycopodiella chinensis extract, 14g of Gallus gallus domesticus extract, 6.5g of Curcuma aromatica extract, 9g of Pinellia ternata extract, 4.5g of Scutellaria baicalensis extract, 7g of Coptis chinensis extract, 6.5g of White Peony Root extract, and 14g of Citrus aurantium extract.

[0081] Example 21

[0082] Formula: 7g of Desmodium sibiricum extract, 16g of Lycopodiella chinensis extract, 6g of Gallus gallus domesticus extract, 16g of Curcuma aromatica extract, 4g of Pinellia ternata extract, 11g of Scutellaria baicalensis extract, 2.5g of Coptis chinensis extract, 16g of White Peony Root extract, and 6g of Citrus aurantium extract.

[0083] Example 22

[0084] Prescription: 20g of Desmodium sibiricum extract, 6g of Lycopodiella chinensis extract, 16g of Gallus gallus domesticus extract, 6g of Curcuma aromatica extract, 11g of Pinellia ternata extract, 4g of Scutellaria baicalensis extract, 7.5g of Coptis chinensis extract, 6g of White Peony Root extract, and 16g of Citrus aurantium extract.

[0085] Example 23

[0086] Formula: 6.5g of Desmodium sabdariffa extract, 20g of Lycopodiella chinensis extract, 5.5g of Gallus gallus domesticus extract, 20g of Curcuma aromatica extract, 3.5g of Pinellia ternata extract, 13g of Scutellaria baicalensis extract, 2.5g of Coptis chinensis extract, 20g of White Peony Root extract, and 5.5g of Citrus aurantium extract.

[0087] Example 24

[0088] Formula: 24g of Desmodium sibiricum extract, 5.5g of Lycopodiella chinensis extract, 20g of Gallus gallus domesticus extract, 5.5g of Curcuma aromatica extract, 13g of Pinellia ternata extract, 3.5g of Scutellaria baicalensis extract, 8g of Coptis chinensis extract, 5.5g of White Peony Root extract, and 20g of Citrus aurantium extract.

[0089] Example 25

[0090] Formula: 6g of Desmodium sibiricum extract, 23g of Lygodium japonicum extract, 5g of Gallus galli extract, 23g of Curcuma aromatica extract, 3g of Pinellia ternata extract, 14g of Scutellaria baicalensis extract, 2g of Coptis chinensis extract, 23g of White Peony Root extract, and 5g of Citrus aurantium extract.

[0091] Example 26

[0092] Formula: 27g of Desmodium sibiricum extract, 5g of Lycopodiella chinensis extract, 23g of Gallus gallus domesticus extract, 5g of Curcuma aromatica extract, 14g of Pinellia ternata extract, 3g of Scutellaria baicalensis extract, 9g of Coptis chinensis extract, 5g of White Peony Root extract, and 23g of Citrus aurantium extract.

[0093] The extracts described in Examples 14-26 above are water extracts of the respective raw materials.

[0094] Example 27

[0095] Prescription: 10g of Houttuynia cordata Granules, 8g of Lycopodiellae Granules, 8g of Chicken Gizzard Granules, 8g of Curcuma Granules, 6g of Pinellia Ternatee Granules, 6g of Scutellaria Baicalensis Granules, 4g of Coptis chinensis Granules, 8g of White Peony Root Granules, and 8g of Citrus aurantium Granules.

[0096] Example 28

[0097] Prescription: 8.5g of Houttuynia cordata Granules, 9g of Lycopodiella ternata Granules, 7.5g of Chicken Gizzard Stone Granules, 9g of Curcuma zedoaria Granules, 5.5g of Pinellia ternata Granules, 7g of Scutellaria baicalensis Granules, 3.5g of Coptis chinensis Granules, 9g of White Peony Root Granules, and 7.5g of Citrus aurantium Granules.

[0098] Example 29

[0099] Prescription: Desmodium chinense Granules 12g, Lycopodiella ternata Granules 7.5g, Chicken Gizzard Stone Granules 9g, Curcuma zedoaria Granules 7.5g, Pinellia ternata Granules 7g, Scutellaria baicalensis Granules 5.5g, Coptis chinensis Granules 6g, White Peony Root Granules 7.5g, Citrus aurantium Granules 9g.

[0100] Example 30

[0101] Prescription: Desmodium leucoderma Granules 8g, Lycopodiella galanga Granules 11g, Chicken Gizzard Granules 7g, Curcuma zedoaria Granules 11g, Pinellia ternata Granules 5g, Scutellaria baicalensis Granules 8g, Coptis chinensis Granules 3g, White Peony Root Granules 11g, Citrus aurantium Granules 7g.

[0102] Example 31

[0103] Prescription: Guangzhencao Granules 14g, Haijinsha Granules 7g, Chicken Gizzard Granules 11g, Curcuma Granules 7g, Qingbanxia Granules 8g, Huangqin Granules 5g, Huanglian Granules 7g, Baishao Granules 7g, Zhiqiao Granules 11g.

[0104] Example 32

[0105] Prescription: Guangzhencao Granules 7.5g, Haijinsha Granules 14g, Chicken Gizzard Granules 6.5g, Curcuma Granules 14g, Qingbanxia Granules 4.5g, Huangqin Granules 9g, Huanglian Granules 3g, Baishao Granules 14g, and Zhiqiao Granules 6.5g.

[0106] Example 33

[0107] Prescription: Desmodium leucoderma Granules 18g, Lycopodiella galanga Granules 6.5g, Chicken Gizzard Stone Granules 14g, Curcuma zedoaria Granules 6.5g, Pinellia ternata Granules 9g, Scutellaria baicalensis Granules 4.5g, Coptis chinensis Granules 7g, White Peony Root Granules 6.5g, Citrus aurantium Granules 14g.

[0108] Example 34

[0109] Prescription: Desmodium chinense Granules 7g, Lycopodiella ternata Granules 16g, Chicken Gizzard Granules 6g, Curcuma zedoaria Granules 16g, Pinellia ternata Granules 4g, Scutellaria baicalensis Granules 11g, Coptis chinensis Granules 2.5g, White Peony Root Granules 16g, Citrus aurantium Granules 6g.

[0110] Example 35

[0111] Prescription: Desmodium chinense Granules 20g, Lycopodiella ternata Granules 6g, Chicken Gizzard Granules 16g, Curcuma zedoaria Granules 6g, Pinellia ternata Granules 11g, Scutellaria baicalensis Granules 4g, Coptis chinensis Granules 7.5g, White Peony Root Granules 6g, Citrus aurantium Granules 16g.

[0112] Example 36

[0113] Prescription: 6.5g of Houttuynia cordata Granules, 20g of Lycopodiella ternata Granules, 5.5g of Chicken Gizzard Stone Granules, 20g of Curcuma zedoaria Granules, 3.5g of Pinellia ternata Granules, 13g of Scutellaria baicalensis Granules, 2.5g of Coptis chinensis Granules, 20g of White Peony Root Granules, and 5.5g of Citrus aurantium Granules.

[0114] Example 37

[0115] Prescription: Desmodium chinense Granules 24g, Lycopodiella galanga Granules 5.5g, Chicken Gizzard Stone Granules 20g, Curcuma zedoaria Granules 5.5g, Pinellia ternata Granules 13g, Scutellaria baicalensis Granules 3.5g, Coptis chinensis Granules 8g, White Peony Root Granules 5.5g, Citrus aurantium Granules 20g.

[0116] Example 38

[0117] Prescription: 6g of Houttuynia cordata Granules, 23g of Lycopodiella ternata Granules, 5g of Chicken Gizzard Stone Granules, 23g of Curcuma zedoaria Granules, 3g of Pinellia ternata Granules, 14g of Scutellaria baicalensis Granules, 2g of Coptis chinensis Granules, 23g of White Peony Root Granules, and 5g of Citrus aurantium Granules.

[0118] Example 39

[0119] Prescription: Desmodium chinense Granules 27g, Lycopodiella galanga Granules 5g, Chicken Gizzard Stone Granules 23g, Curcuma zedoaria Granules 5g, Pinellia ternata Granules 14g, Scutellaria baicalensis Granules 3g, Coptis chinensis Granules 9g, White Peony Root Granules 5g, Citrus aurantium Granules 23g.

[0120] The granules described in Examples 27-39 above are granules prepared by adding an appropriate amount of dextrin to the water extract of each raw material drug.

[0121] Effect Example 1: Observation of the therapeutic effect of the Chinese medicine composition of the present invention on guinea pig cholecystitis

[0122] 1 Experimental Purpose

[0123] The therapeutic effect of the Chinese medicinal composition of the present invention on lincomycin hydrochloride-induced cholecystitis in guinea pigs was studied.

[0124] 2 Experimental Materials

[0125] 2.1 Experimental animals

[0126] Sixty clean-grade guinea pigs, half male and half female, weighing (350 ± 50) g, were housed in separate cages, 5 per cage, and fed a standard solid diet (purchased from Beijing Keao Feed Company) with free access to water. They were housed in an environment with a 12-h light cycle, room temperature of 24.2°C, and humidity of 44.2%.

[0127] 2.2 Experimental drugs

[0128] The Chinese medicine composition of the present invention is a granule prepared according to Example 1. Positive control drug: Xiaoyan Lidan Tablets (Guangdong Luofushan Chinese Medicine Co., Ltd., batch number: F20A005). Modeling drug: Lincomycin Hydrochloride (Shanghai Xudong Haipu Pharmaceutical Co., Ltd., batch number: AM191006).

[0129] 3 Experimental methods

[0130] 3.1 Animal grouping

[0131] Sixty guinea pigs weighing (350±50) g, half male and half female, were raised in separate cages. After 7 days of adaptive feeding, all animals were divided into a blank control group, a model group, a positive control group, and high-, medium-, and low-dose groups of the Chinese medicine composition of the present invention using a random number table method, with 10 animals in each group.

[0132] 3.2 Animal model preparation

[0133] Guinea pig cholecystitis model was established according to the literature method. Except for the blank control group, the other groups were subcutaneously injected with lincomycin hydrochloride 30 mg kg -1 The guinea pig cholecystitis model was established by injection of the same volume of purified water once a day for 7 consecutive days.

[0134] 3.3 Drug intervention

[0135] On the 7th day after injection, the patients were divided into groups and given oral administration. The positive control group (0.36 g·kg -1 ), the low-dose group, medium-dose group, high-dose group, blank control group, and model group of the Chinese medicine composition of the present invention were given the same volume of purified water every day for 30 consecutive days. According to the table of equivalent dose coefficients of body surface area conversion between different animals in "Experimental Animal Science", the conversion coefficient between a human with a body mass of 70 kg and a guinea pig with a body mass of 0.4 kg is 0.031. Combined with the ratio, the dosage of the guinea pig in this experiment was calculated according to the formula: low dose: 3.29 g·kg -1 Medium dose: 6.59 g·kg -1 , high dose: 13.18 g·kg -1 .

[0136] 3.4 Collection of materials

[0137] On the second day after the last administration, the guinea pigs were weighed and the dose was calculated as 3.5 mL·kg -1 Guinea pigs were anesthetized with an intraperitoneal injection of 10% chloral hydrate, the abdominal cavity was opened, blood was collected from the abdominal aorta, the gallbladder was ligated, the gallbladder was removed to extract bile, and the liver was removed.

[0138] 4. Detection indicators and methods

[0139] 4.1 General Condition of Guinea Pigs

[0140] After successful modeling, the mental state, fur glossiness, food intake, defecation volume, and stool morphology of each group of rats were observed. The body weight of each rat was measured after modeling and after Chinese medicine treatment. The bile volume of each group of guinea pigs was measured.

[0141] 4.2 Serological indicators

[0142] Blood was collected from the abdominal aorta and allowed to stand at room temperature for 2 h. The blood was then centrifuged at 3000 r / min for 15 min at 4°C to separate the upper serum layer. The levels of TNF-α and IL-1β were determined according to the instructions of the ELISA test kit.

[0143] 4.3 Liver function

[0144] Guinea pig serum was collected and liver function indicators including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), glutamyl transaminase (GGT), total bilirubin (TBIL), and total bile acid (TBA) were measured.

[0145] 5 Statistical methods

[0146] SPSS 26.0 software was used to perform statistical analysis on the experimental data, and the experimental results were expressed as mean ± standard deviation. When the data conformed to a normal distribution and had homogeneous variance, one-way analysis of variance and LSD-t-test were used; when the data did not conform to a normal distribution, nonparametric tests were used. P < 0.05 indicated statistical significance.

[0147] 6 Results

[0148] 6.1 Comparison of general conditions of guinea pigs in each group

[0149] After modeling, the guinea pigs showed slow movement, dull and easy-to-fall fur, arched back, huddled together, yellow urine, and loose stools. After drug intervention, the defecation and urination conditions, daily activities, fur glossiness, and mental state of the guinea pigs in the anti-inflammatory and choleretic tablets group and the Chinese medicine composition group were significantly improved compared with those in the model group.

[0150] After modeling, the body weight of the model guinea pigs was significantly lower than that of the normal group. After treatment, the body weights of the guinea pigs in the Xiaoyan Lidan Tablets group and the Chinese medicine composition group of the present invention were significantly higher than that of the model group (P < 0.01), and the body weight of the medium-dose Chinese medicine composition group of the present invention was higher than that of the Xiaoyan Lidan Tablets group (P < 0.05). The results are shown in Table 1.

[0151] Table 1 Body weight of guinea pigs in each group at different times

[0152] Group Initial body weight (g) Body weight after modeling (g) Body weight after treatment (g) Normal group 352.88±16.07 405.73±12.59 526.83±21.18 Model Group 350.13±15.13 <![CDATA[373.52±18.32 ** ]]> <![CDATA[423.41±22.99 ** ]]> Anti-inflammatory and choleretic tablets 358.59±14.88 <![CDATA[372.15±17.83 ** ]]> <![CDATA[469.22±26.01 **## ]]> Low-dose group of Chinese medicine composition 341.77±19.14 <![CDATA[376.56±18.30 ** ]]> <![CDATA[461.68±21.00 **## ]]> Chinese medicine composition medium dose group 349.77±13.97 <![CDATA[367.73±16.81 ** ]]> <![CDATA[491.23±23.70 **##& ]]> High-dose group of Chinese medicine composition 358.78±15.09 <![CDATA[377.11±20.27 ** ]]> <![CDATA[488.06±24.49 **## ]]>

[0153] Note: Compared with the normal group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the Xiaoyan Lidan tablets group, & P<0.05, && P<0.05.

[0154] The bile volumes of guinea pigs in each group are shown in Table 2. Compared with the blank group, the bile volumes of the other groups were significantly increased. After treatment, the bile volumes of the Xiaoyan Lidan Tablets and the Chinese medicine composition group of the present invention were significantly reduced. Among them, the medium-dose group and the high-dose group of the Chinese medicine composition group of the present invention were equivalent to the Xiaoyan Lidan Tablets.

[0155] Table 2 Comparison of bile volume in different groups of guinea pigs

[0156] Group Bile volume (ml) Normal group 0.42±0.1 Model Group <![CDATA[1.369±0.39723 ** ]]> Anti-inflammatory and choleretic tablets <![CDATA[0.69±0.15972 **## ]]> Low-dose group of Chinese medicine composition <![CDATA[0.92±0.10011 **##& ]]> Chinese medicine composition medium dose group <![CDATA[0.61±0.11991 *## ]]> High-dose group of Chinese medicine composition <![CDATA[0.71±0.1699 **## ]]>

[0157] Note: Compared with the normal group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the Xiaoyan Lidan tablets group, & P<0.05, && P<0.05.

[0158] 6.2 Serological indicators

[0159] As shown in Table 3, compared with the blank group, TNF-α and IL-1β in the other groups were significantly increased. Compared with the model group, the levels of TNF-α and IL-1β in the Xiaoyan Lidan Tablet group and the Chinese medicine composition group of the present invention were significantly decreased. Among them, the levels of TNF-α and IL-1β in the medium dose group of the Chinese medicine composition group of the present invention were significantly decreased compared with the Xiaoyan Lidan Tablet group.

[0160] Table 3 Comparison of TNF-α and IL-1β in serum of guinea pigs in different groups

[0161] Group TNF-α (pg / ml) IL-1β (pg / ml) Normal group 294.20±43.99 3.90±0.62 Model Group <![CDATA[446.62±63.01 ** ]]> <![CDATA[23.70±3.34 ** ]]> Anti-inflammatory and choleretic tablets <![CDATA[377.76±20.61 **## ]]> <![CDATA[14.29±2.91 **## ]]> Low-dose group of Chinese medicine composition <![CDATA[363.09±38.59 **## ]]> <![CDATA[16.20±3.04 **## ]]> Chinese medicine composition medium dose group <![CDATA[336.66±22.22 *##& ]]> <![CDATA[10.35±2.43 **##&& ]]> High-dose group of Chinese medicine composition <![CDATA[349.82±30.57 **## ]]> <![CDATA[12.11±2.13 **## ]]>

[0162] Note: Compared with the normal group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the Xiaoyan Lidan tablets group, & P<0.05, && P<0.05.

[0163] 6.3 Liver Function

[0164] The effects of each group on liver function in guinea pigs with cholecystitis are shown in Table 4. AST, ALT, ALP, GGT, TBIL, and TBA in the model group were all elevated compared to the normal group (P < 0.01), indicating that lincomycin severely damages the liver. Treatment with different doses of the Xiaoyan Lidan Tablets and the Chinese herbal composition of the present invention resulted in some recovery of liver function. Specifically, AST, GGT, TBIL, and TBA in the medium-dose group of the Chinese herbal composition of the present invention, as well as TBIL and TBA in the high-dose group, all showed significant decreases compared to the Xiaoyan Lidan Tablets group.

[0165] Table 4 Comparison of liver function related indicators in different groups of guinea pigs

[0166] Group AST ALT ALP Normal group 70.67±15.33 73.81±16.40 41.82±10.44 Model Group <![CDATA[209.66±49.25 ** ]]> <![CDATA[126.54±18.31 ** ]]> <![CDATA[116.65±27.54 ** ]]> Anti-inflammatory and choleretic tablets <![CDATA[131.39±22.98 **## ]]> <![CDATA[94.73±16.80 **## ]]> <![CDATA[77.59±19.33 **## ]]> Low-dose group <![CDATA[140.66±23.49 **## ]]> <![CDATA[91.49±19.21 **## ]]> <![CDATA[75.28±17.42 **## ]]> Medium dose group <![CDATA[105.50±22.29 **##& ]]> <![CDATA[83.75±13.50 ## ]]> <![CDATA[62.23±12.78 *## ]]> High-dose group <![CDATA[109.31±24.38 **## ]]> <![CDATA[85.29±14.60 ## ]]> <![CDATA[72.19±14.49 **## ]]> Group GGT TBIL TBA Normal group 23.30±4.78 0.61±0.21 6.52±1.56 Model Group <![CDATA[66.41±10.43 ** ]]> <![CDATA[2.07±0.42 ** ]]> <![CDATA[29.60±5.88 ** ]]> Anti-inflammatory and choleretic tablets <![CDATA[52.50±12.33 **## ]]> <![CDATA[1.41±0.27 **## ]]> <![CDATA[19.29±2.76 **## ]]> Low-dose group <![CDATA[55.32±11.79 **# ]]> <![CDATA[1.35±0.22 **## ]]> <![CDATA[16.32±3.10 **##& ]]> Medium dose group <![CDATA[41.73±10.41 **##& ]]> <![CDATA[1.01±0.19 **##&& ]]> <![CDATA[12.13±2.50 **##&& ]]> High-dose group <![CDATA[44.72±12.60 **## ]]> <![CDATA[1.12±0.23 **##& ]]> <![CDATA[11.85±2.23 **##&& ]]>

[0167] Note: Compared with the normal group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with the Xiaoyan Lidan tablets group, & P<0.05, && P<0.05.

[0168] Effect Example 2: Observation of the therapeutic effect of different Chinese medicine compositions on guinea pig cholecystitis

[0169] 1 Experimental Materials

[0170] 1.1 Experimental animals

[0171] Fifty clean-grade guinea pigs, half male and half female, weighing (350 ± 50) g, were housed in separate cages, fed a standard solid diet (purchased from Beijing Keao Feed Company) with free access to water. They were housed in an environment with a 12-h light cycle, room temperature of 24.2°C, and humidity of 44.2%.

[0172] 1.2 Experimental drugs

[0173] Experimental drugs ①: Herba Lysimachiae 10g, Herba Lygodii 8g, Herba Galli 8g, Herba Curcumae 8g, Herba Pinelliae 6g, Herba Scutellariae 6g, Herba Coptis 4g, Herba Paeoniae Albae 8g, Fructus Aurantii Immaturus 8g;

[0174] Experimental drug ②: Herba Lysimachiae 10g, Herba Lygodii 8g, Herba Galli 8g, Herba Curcumae 8g, Herba Pinelliae 9g, Herba Scutellariae 3g, Herba Coptis 2g, Herba Paeoniae Albae 8g, Fructus Aurantii Immaturus 8g;

[0175] Experimental drug ③: Herba Lysimachiae 10g, Herba Lygodii 8g, Herba Galli 8g, Herba Curcumae 8g, Herba Pinelliae 3g, Herba Scutellariae 9g, Herba Coptis 5g, Herba Paeoniae Albae 8g, Fructus Aurantii Immaturus 8g;

[0176] Each group of drugs was prepared into granules according to Example 1.

[0177] Modeling drug: Lincomycin hydrochloride (Shanghai Xudong Haipu Pharmaceutical Co., Ltd., batch number: AM191006).

[0178] 2 Experimental methods

[0179] Fifty guinea pigs weighing (350±50) g ​​were selected, half male and half female, and housed in separate cages. After 7 days of adaptive feeding, all animals were divided into blank control group, model group, experimental drug group ①, experimental drug group ②, and experimental drug group ③ using a random number table method, with 10 animals in each group. Each group was subcutaneously injected with lincomycin hydrochloride 30 mg·kg -1 , once a day for 7 consecutive days to replicate the guinea pig cholecystitis model. From the 7th day after injection, each experimental drug group was given 6.59 g·kg -1 The blank control group and the model group were given the same volume of purified water every day for 30 days. On the second day after the last administration, the guinea pigs were weighed and the 3.5 mL·kg -1 Guinea pigs were anesthetized with an intraperitoneal injection of 10% chloral hydrate. The abdominal cavity was opened, blood was collected from the abdominal aorta, and the gallbladder was ligated. The gallbladder was removed for bile extraction, and the liver was removed. The bile volume of each guinea pig group was measured. Serum was collected and liver function markers, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), were measured.

[0180] 3 Results

[0181] 3.1 Comparison of general conditions of guinea pigs in each group

[0182] The guinea pigs in the model group had severe loose stools. The guinea pigs in experimental drug groups ① and ② had normal stools after drug intervention. The guinea pigs in experimental drug group ③ had no improvement in loose stools.

[0183] 3.2 Comparison of bile volume in each group of guinea pigs

[0184] The bile volumes of guinea pigs in each group are shown in Table 5. After treatment, the bile volumes of all experimental drug groups were significantly reduced, and the effect of experimental drug group ① was significantly better than that of experimental drug groups ② and ③.

[0185] Table 5 Comparison of bile volume in different groups of guinea pigs

[0186] Group Bile volume (ml) Normal group 0.44±0.08 Model Group <![CDATA[1.31±0.38 ** ]]> Experimental drug group ① <![CDATA[0.62±0.15 ## ]]> Experimental drug group ② <![CDATA[0.93±0.25 **##&& ]]> Experimental drug group ③ <![CDATA[0.89±0.22 **##& ]]>

[0187] Note: Compared with the normal group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with experimental drug group ①,& P<0.05, && P<0.01.

[0188] 3.3 Liver function

[0189] The effects of each group on the liver function of guinea pigs with cholecystitis are shown in Table 6.

[0190] Table 6 Comparison of liver function related indicators in different groups of guinea pigs

[0191] Group AST ALT ALP Normal group 68.42±17.07 78.26±15.83 43.89±12.28 Model Group <![CDATA[202.61±44.08 ** ]]> <![CDATA[134.64±20.42 ** ]]> <![CDATA[121.60±27.22 ** ]]> Experimental drug group ① <![CDATA[124.34±19.74 **## ]]> <![CDATA[83.00±15.20 ## ]]> <![CDATA[68.69±15.67 **## ]]> Experimental drug group ② <![CDATA[153.01±27.12 **##& ]]> <![CDATA[94.91±17.44 *## ]]> <![CDATA[86.03±14.12 **##& ]]> Experimental drug group ③ <![CDATA[160.25±26.54 **##&& ]]> <![CDATA[98.99±14.88 **##& ]]> <![CDATA[89.29±21.74 **##& ]]>

[0192] Note: Compared with the normal group, * P<0.05, ** P<0.01; compared with the model group, # P<0.05, ## P<0.01; compared with experimental drug group ①, & P<0.05, && P<0.05.

[0193] The experimental results showed that AST, ALT, and ALP in the model group were all higher than those in the normal group (P<0.01), indicating that lincomycin severely damaged the liver. After treatment with each experimental drug, liver function recovered to a certain extent. Specifically, AST, ALT, and ALP in experimental drug group ① were significantly lower than those in experimental drug groups ② and ③, indicating that experimental drug ① had a significantly better protective effect on liver function damage in guinea pigs with cholecystitis than experimental drugs ② and ③.

Claims

1. A Chinese medicine composition for treating cholecystitis, characterized in that: The traditional Chinese medicine composition is prepared from dwarf lily herb, sea sand, chicken's gizzard lining, turmeric, pinellia, scutellaria, coptis root, white peony root and fructus aurantii.

2. The Chinese medicine composition according to claim 1, wherein The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 6-30 parts by weight of Desmodium sibiricum, 5-25 parts by weight of Lygodium japonicum, 5-25 parts by weight of Gallus gallus domesticus, 5-25 parts by weight of Curcuma aromatica, 3-15 parts by weight of Pinellia ternata, 3-15 parts by weight of Scutellaria baicalensis, 2-10 parts by weight of Coptis chinensis, 5-25 parts by weight of White Peony Root, and 5-25 parts by weight of Fructus Aurantii Immaturus.

3. The Chinese medicine composition according to claim 2, wherein The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 7-22 parts by weight of Desmodium sibiricum, 6-18 parts by weight of Lygodium japonicum, 6-18 parts by weight of Gallus gallus domesticus, 6-18 parts by weight of Curcuma aromatica, 4-12 parts by weight of Pinellia ternata, 4-12 parts by weight of Scutellaria baicalensis, 2.5-7.5 parts by weight of Coptis chinensis, 6-18 parts by weight of White Peony Root, and 6-18 parts by weight of Fructus Aurantii Immaturus.

4. The Chinese medicine composition according to claim 3, wherein The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 8-15 parts by weight of Desmodium sibiricum, 7-12 parts by weight of Lygodium japonicum, 7-12 parts by weight of Chicken's Gizzard Stone, 7-12 parts by weight of Curcuma aromatica, 5-8 parts by weight of Pinellia ternata, 5-8 parts by weight of Scutellaria baicalensis, 3-4 parts by weight of Coptis chinensis, 7-12 parts by weight of White Peony Root, and 7-12 parts by weight of Fructus Aurantii Immaturus.

5. The Chinese medicine composition according to claim 4, characterized in that The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 parts by weight of Herba Lygodii, 8 parts by weight of Lygodium japonicum, 8 parts by weight of Gallus galli, 8 parts by weight of Curcuma aromatica, 6 parts by weight of Pinellia ternata, 6 parts by weight of Scutellaria baicalensis, 4 parts by weight of Coptis chinensis, 8 parts by weight of White Peony Root, and 8 parts by weight of Fructus Aurantii Immaturus.

6. The Chinese medicine composition according to any one of claims 1 to 5, characterized in that The Chinese medicine composition is a composition formed by mixing the raw materials after crushing, or an extract obtained by mixing or extracting the raw materials separately, or an effective part obtained by further refining and purifying the extract, or a conventional dosage form prepared by adding pharmaceutically acceptable excipients to the extract / effective part.

7. A Chinese medicine composition for treating cholecystitis, characterized in that: The traditional Chinese medicine composition is prepared from the following raw materials: 6-30 parts by weight of Desmodium sibiricum extract, 5-25 parts by weight of Lygodium japonicum extract, 5-25 parts by weight of Gallus gallifolia extract, 5-25 parts by weight of Curcuma aromatica extract, 3-15 parts by weight of Pinellia ternata extract, 3-15 parts by weight of Scutellaria baicalensis extract, 2-10 parts by weight of Coptis chinensis extract, 5-25 parts by weight of White Peony Root extract, and 5-25 parts by weight of Fructus Aurantii Immaturus extract.

8. The Chinese medicine composition according to claim 7, characterized in that The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 7-22 parts by weight of Desmodium sibiricum extract, 6-18 parts by weight of Lygodium japonicum extract, 6-18 parts by weight of Gallus gallifolia extract, 6-18 parts by weight of Curcuma aromatica extract, 4-12 parts by weight of Pinellia ternata extract, 4-12 parts by weight of Scutellaria baicalensis extract, 2.5-7.5 parts by weight of Coptis chinensis extract, 6-18 parts by weight of White Peony Root extract, and 6-18 parts by weight of Fructus Aurantii Immaturus extract.

9. The Chinese medicine composition according to claim 8, characterized in that The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 parts by weight of Desmodium sibiricum extract, 8 parts by weight of Lygodium japonicum extract, 8 parts by weight of Gallus gallifolia extract, 8 parts by weight of Curcuma aromatica extract, 6 parts by weight of Pinellia ternata extract, 6 parts by weight of Scutellaria baicalensis extract, 4 parts by weight of Coptis chinensis extract, 8 parts by weight of White Peony Root extract, and 8 parts by weight of Fructus Aurantii Immaturus extract.

10. Use of the traditional Chinese medicine composition according to claims 1-5, 7-9 in preparing medicine for treating cholecystitis.