A wound antiseptic gel and method of preparation thereof

Abstract

The present application relates to the technical field of wound gel, in particular to a kind of wound antibacterial disinfectant gel and preparation method thereof.Preparation raw materials of the wound antibacterial disinfectant gel include the following components by weight: PCA-HBPG 4-6 parts, modified chitosan 6-9 parts, 3-amino phenylboric acid 1.5-2 parts, iodine 0.6-0.9 parts, potassium iodide 1.2-1.8 parts, curcumin 0.8-1 part, and the prepared antibacterial disinfectant gel has good bacteriostatic effect, and can promote wound healing.

Description

Technical Field

[0001] The present invention relates to the technical field of wound gels, and in particular to an antibacterial and disinfecting wound gel and a preparation method thereof. Background Art

[0002] The main function of the skin is to protect it, preventing the invasion of pathogenic microorganisms in the environment and the loss of its own body fluids. Normal skin is affected by external factors such as surgery, external force, chemicals, and internal factors such as local blood supply disorders, which can cause trauma to the skin surface. This process is often accompanied by the destruction of skin integrity and the loss of certain normal tissues, and at the same time, the normal function of the skin is affected and damaged to a certain extent. At present, wound repair products mainly include medical wound gels, but they often cause wounds to dry out, destroy healthy growth factors, and the wound healing time is long, which can easily cause infection. Although iodine, hydrogen peroxide, alcohol, etc. can quickly disinfect, they are irritating, lack long-term effectiveness, and have poor healing effects. The current silver ion disinfectant gel is directly used for wound disinfection and wound healing, which can avoid secondary damage. Silver ions have antibacterial properties, but require a higher silver concentration and have a short antibacterial effect. Summary of the Invention

[0003] In view of the deficiencies in the prior art, the present invention provides an antibacterial and disinfecting gel for wound surfaces and a preparation method thereof.

[0004] The present invention is achieved through the following technical solutions:

[0005] The invention discloses an antibacterial and disinfecting gel for wounds. The raw materials for preparing the gel include the following components in parts by weight: 4-6 parts of PCA-HBPG, 6-9 parts of modified chitosan, 1.5-2 parts of 3-aminophenylboronic acid, 0.6-0.9 parts of iodine, 1.2-1.8 parts of potassium iodide, and 0.8-1 part of curcumin.

[0006] Furthermore, the preparation method of PCA-HBPG comprises the following steps:

[0007] L1. Mix glycerol and sodium methoxide, stir and react at 30°C for 1 hour. Then, heat to 60°C, evacuate, add 1,4-dioxane, and heat to 95°C. Add glycidol dropwise with stirring. After the addition is complete, react for 5 hours. Remove 1,4-dioxane by rotary evaporation. Dialyze against deionized water for 3 days and freeze-dry to obtain HBPG.

[0008] L2. Under a nitrogen atmosphere, add protocatechuic acid and the HBPG obtained in step L1 to DMSO and stir to dissolve. Add EDC·HCl and DMAP, and react with stirring at 30°C in the dark for 24 h. Filter and dialyze the filtrate against DMSO for 48 h and then against deionized water for 72 h. Freeze-dry to obtain PCA-HBPG.

[0009] Furthermore, in step L1, the molar ratio of the glycerol, sodium methoxide and glycidol is 1:0.1:21.

[0010] Furthermore, in step L1, the usage ratio of glycerol to 1,4-dioxane is 0.1 mol:20 mL.

[0011] Furthermore, in step L2, the mass concentration of protocatechuic acid in DMSO is 10 mg / mL.

[0012] Furthermore, in step L2, the mass ratio of protocatechuic acid, HBPG, EDC·HCl and DMAP is 1:2:1.5:0.16.

[0013] Furthermore, the preparation method of the modified chitosan comprises the following steps:

[0014] V1. Dissolve NaIO4 in deionized water to obtain a 5% w / v NaIO4 solution. Dissolve asiatic acid in anhydrous ethanol. Add the NaIO4 solution dropwise. Adjust the pH to 3-4 with dilute hydrochloric acid. Stir the reaction at room temperature for 1 h. After the reaction, extract and wash repeatedly with ethyl acetate and saturated brine. Combine the organic phases, dry over anhydrous MgSO4, filter, and evaporate the solvent under reduced pressure.

[0015] V2. Add chitosan to a 1% v / v acetic acid solution and stir at 600 r / min for 2 h to obtain a CS solution. Dissolve the modified Centella asiatica acid obtained in step V1 in ethanol. Add EDC·HCl and NHS, adjust the pH to 5, and stir for 2 h. Mix with the CS solution and react with stirring for 24 h. Dialyze in deionized water for 3 days, freeze-dry, wash with anhydrous ethanol, and dry in a vacuum oven to obtain modified chitosan.

[0016] Furthermore, in step V1, the mass concentration of asiatic acid in anhydrous ethanol is 2 mg / mL.

[0017] Furthermore, in step V1, the mass ratio of asiatic acid to NaIO4 is 3:2.

[0018] Furthermore, in step V2, the mass concentration of the chitosan in the 1% v / v acetic acid solution is 20 mg / mL.

[0019] Furthermore, in step V2, the mass ratio of the modified Centella asiatica acid to chitosan is 1:10.

[0020] Furthermore, in step V2, the mass concentration of the modified Centella asiatica acid in ethanol is 2 mg / mL.

[0021] Furthermore, in step V2, the mass ratio of the modified Centella asiatica acid, EDC·HCl and NHS is 1:0.5:0.3.

[0022] Furthermore, the present invention also provides a method for preparing the wound antibacterial disinfection gel, comprising the following steps:

[0023] Iodine and potassium iodide were added to deionized water and mixed to obtain iodine solution. Modified chitosan was dissolved in 1% v / v acetic acid solution to obtain modified chitosan solution. 3-Aminophenylboronic acid was added to a PBS solution with a pH of 7.4 and ultrasonically dissolved. PCA-HBPG was added, and the mixture was heated in a water bath at 60°C and stirred at 600 rpm for 1 h. A 10 mg / mL ethanol solution of curcumin and the modified chitosan solution were added and stirred evenly. The pH value was adjusted to 7.4, and the mixture was stirred at 300 rpm at room temperature for 1 h. The mixture was allowed to stand for 6 h, and iodine solution was added. The mixture was allowed to stand at 4°C for 12 h. The ethanol was removed under reduced pressure, and the mixture was washed with deionized water. The surface moisture was absorbed with filter paper to obtain an antibacterial and disinfectant gel for wounds.

[0024] Furthermore, the mass concentration of the modified chitosan in 1% v / v acetic acid solution is 20 mg / mL.

[0025] Furthermore, the mass concentration of the potassium iodide in deionized water is 40 mg / mL.

[0026] Furthermore, the mass concentration of the 3-aminophenylboronic acid in the PBS solution with a pH of 7.4 is 5 mg / mL.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The present invention provides an antibacterial wound disinfection gel. PCA-HBPG cross-linked chitosan is modified and combined with iodine, curcumin, and other ingredients to achieve effective antibacterial properties and accelerate wound healing. PCA-HBPG is prepared by glycidol polymerization to produce a hydroxyl-rich hyperbranched glycidyl ether, HBPG. HBPG exhibits excellent hydrophilicity and biocompatibility. The carboxyl groups of protocatechuic acid (PCA) react with the hydroxyl groups of HBPG to graft PCA onto HBPG. Catechol groups are then introduced into HBPG, providing a foundation for subsequent boronate ester crosslinking. The present invention prepares modified chitosan. First, sodium periodate (NaIO4) is used to oxidize the o-dihydroxyl groups in asiatic acid to aldehyde groups, yielding modified asiatic acid. This provides a basis for a subsequent Schiff-base crosslinking reaction with amino groups. The carboxyl groups of the modified asiatic acid react with amino groups on chitosan to form an amide reaction, introducing the modified asiatic acid groups into chitosan. Asiatic acid is a hydrophobic compound, allowing the introduction of hydrophobic side chains into chitosan, creating hydrophobic interactions with the hydrophobic drug curcumin, facilitating the loading of the active ingredient. Simultaneously, the introduction of aldehyde groups into chitosan facilitates subsequent crosslinking to form a gel. The present invention utilizes 3-aminophenylboronic acid to form boronate ester bonds with the catechol groups of PCA-HBPG. The amino groups further form Schiff-base bonds with the aldehyde groups of the modified chitosan, successfully crosslinking HBPG and chitosan to form a gel. The resulting Schiff-base bonds exhibit iodine complexation activity. Iodine has a broad-spectrum bactericidal effect, rapidly killing pathogenic microorganisms such as bacteria and fungi. Curcumin has antioxidant properties, effectively inhibiting bacteria and promoting wound healing. The present invention effectively loads iodine into the gel, and curcumin is loaded into the gel through interaction with the modified chitosan side chain, thereby improving the sustained release and stability of the active ingredient, enhancing the long-term antibacterial effect, and improving the wound healing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0030] Figure 1 The antibacterial properties of the gels described in Examples 1-3 and Comparative Examples 1-2 of the present invention;

[0031] Figure 2 The antibacterial stability of the gels described in Example 1 and Comparative Examples 1-2 of the present invention;

[0032] Figure 3 The wound healing activity of the gels described in Example 1 of the present invention and Comparative Examples 1-2. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below in conjunction with specific examples, but the present invention is not limited to the following examples. It should be noted that, unless otherwise specified, the chemical reagents involved in the present invention were purchased through commercial channels.

[0034] Example 1: An antibacterial and disinfectant gel for wounds, prepared from raw materials comprising the following components in parts by weight: 6 parts of PCA-HBPG, 9 parts of modified chitosan, 2 parts of 3-aminophenylboronic acid, 0.9 parts of iodine, 1.8 parts of potassium iodide, and 1 part of curcumin.

[0035] The preparation method of PCA-HBPG comprises the following steps:

[0036] L1. Mix 0.1 mol of glycerol and 0.01 mol of sodium methoxide, stir at 30°C for 1 h, then heat to 60°C, evacuate, add 20 mL of 1,4-dioxane, and heat to 95°C. Add 2.1 mol of glycidol dropwise with stirring. After the addition is complete, react for 5 h. Remove the 1,4-dioxane by rotary evaporation. Dialyze the mixture against deionized water for 3 days and freeze-dry to obtain HBPG.

[0037] L2. Under a nitrogen atmosphere, add 1 g of protocatechuic acid and 2 g of HBPG obtained in step L1 to 100 mL of DMSO and stir to dissolve. Add 1.5 g of EDC·HCl and 0.16 g of DMAP. Incubate with stirring at 30°C in the dark for 24 h. Filter and dialyze the filtrate against DMSO for 48 h and then against deionized water for 72 h. Freeze-dry to obtain PCA-HBPG.

[0038] The preparation method of modified chitosan comprises the following steps:

[0039] V1. Dissolve 0.2 g of NaIO4 in 4 mL of deionized water to obtain a 5% w / v NaIO4 solution. Dissolve 0.3 g of asiatic acid in 150 mL of anhydrous ethanol. Add the NaIO4 solution dropwise, adjust the pH to 4 with dilute hydrochloric acid, and stir at room temperature for 1 h. After the reaction, extract and wash repeatedly with ethyl acetate and saturated brine. Combine the organic phases, dry over anhydrous MgSO4, filter, and evaporate the solvent under reduced pressure.

[0040] V2. Add 1 g of chitosan to 50 mL of 1% v / v acetic acid solution and stir at 600 rpm for 2 h to obtain CS solution. Dissolve 0.1 g of the modified Centella asiatica acid obtained in step V1 in 50 mL of ethanol. Add 0.05 g of EDC·HCl and 0.03 g of NHS, adjust the pH to 5, and stir for 2 h. Mix with the CS solution and react with stirring for 24 h. Dialyze in deionized water for 3 days, freeze-dry, wash with anhydrous ethanol, and dry in vacuo to obtain modified chitosan.

[0041] This embodiment also provides a method for preparing the wound antibacterial disinfection gel, comprising the following steps:

[0042] 0.9 g of iodine and 1.8 g of potassium iodide were added to 45 mL of deionized water and mixed to obtain iodine solution. 9 g of modified chitosan was dissolved in 450 mL of 1% v / v acetic acid solution to obtain 6 g of modified chitosan solution. 2 g of 3-aminophenylboronic acid was added to 400 mL of PBS solution with a pH of 7.4 and dissolved by ultrasonication. PCA-HBPG was added, and the mixture was heated in a water bath at 60°C and stirred at 600 rpm for 1 h. 100 mL of a 10 mg / mL ethanol solution of curcumin was added to the modified chitosan solution and stirred evenly. The pH value was adjusted to 7.4, and the mixture was stirred at 300 rpm at room temperature for 1 h. The mixture was allowed to stand for 6 h, and iodine solution was added. The mixture was allowed to stand at 4°C for 12 h. The ethanol was removed under reduced pressure, and the mixture was washed with deionized water. The surface moisture was absorbed with filter paper to obtain an antibacterial and disinfectant gel for wounds.

[0043] Example 2: An antibacterial and disinfectant gel for wounds, prepared from raw materials comprising the following components in parts by weight: 4 parts of PCA-HBPG, 6 parts of modified chitosan, 1.5 parts of 3-aminophenylboronic acid, 0.6 parts of iodine, 1.2 parts of potassium iodide, and 0.8 parts of curcumin.

[0044] The preparation method of PCA-HBPG comprises the following steps:

[0045] L1. Mix 0.1 mol of glycerol and 0.01 mol of sodium methoxide, stir at 30°C for 1 h, then heat to 60°C, evacuate, add 20 mL of 1,4-dioxane, and heat to 95°C. Add 2.1 mol of glycidol dropwise with stirring. After the addition is complete, react for 5 h. Remove the 1,4-dioxane by rotary evaporation. Dialyze the mixture against deionized water for 3 days and freeze-dry to obtain HBPG.

[0046] L2. Under a nitrogen atmosphere, add 1 g of protocatechuic acid and 2 g of HBPG obtained in step L1 to 100 mL of DMSO and stir to dissolve. Add 1.5 g of EDC·HCl and 0.16 g of DMAP. Incubate with stirring at 30°C in the dark for 24 h. Filter and dialyze the filtrate against DMSO for 48 h and then against deionized water for 72 h. Freeze-dry to obtain PCA-HBPG.

[0047] The preparation method of modified chitosan comprises the following steps:

[0048] V1. Dissolve 0.2 g of NaIO4 in 4 mL of deionized water to obtain a 5% w / v NaIO4 solution. Dissolve 0.3 g of asiatic acid in 150 mL of anhydrous ethanol. Add the NaIO4 solution dropwise, adjust the pH to 3 with dilute hydrochloric acid, and stir at room temperature for 1 h. After the reaction, extract and wash repeatedly with ethyl acetate and saturated brine. Combine the organic phases, dry over anhydrous MgSO4, filter, and evaporate the solvent under reduced pressure.

[0049] V2. Add 1 g of chitosan to 50 mL of 1% v / v acetic acid solution and stir at 600 rpm for 2 h to obtain CS solution. Dissolve 0.1 g of the modified Centella asiatica acid obtained in step V1 in 50 mL of ethanol. Add 0.05 g of EDC·HCl and 0.03 g of NHS, adjust the pH to 5, and stir for 2 h. Mix with the CS solution and react with stirring for 24 h. Dialyze in deionized water for 3 days, freeze-dry, wash with anhydrous ethanol, and dry in vacuo to obtain modified chitosan.

[0050] This embodiment also provides a method for preparing the wound antibacterial disinfection gel, comprising the following steps:

[0051] 0.6 g of iodine and 1.2 g of potassium iodide were added to 30 mL of deionized water and mixed to obtain iodine solution. 6 g of modified chitosan was dissolved in 300 mL of 1% v / v acetic acid solution to obtain modified chitosan solution. 1.5 g of 3-aminophenylboronic acid was added to 300 mL of PBS solution with a pH of 7.4 and dissolved by ultrasonication. 4 g of PCA-HBPG was added, and the mixture was heated in a water bath at 60°C and stirred at 600 rpm for 1 h. 80 mL of a 10 mg / mL ethanol solution of curcumin was added to the modified chitosan solution and stirred evenly. The pH value was adjusted to 7.4, and the mixture was stirred at 300 rpm at room temperature for 1 h. The mixture was allowed to stand for 6 h, and iodine solution was added. The mixture was allowed to stand at 4°C for 12 h. The ethanol was removed under reduced pressure, and the mixture was washed with deionized water. The surface moisture was absorbed with filter paper to obtain an antibacterial and disinfectant gel for wound surface.

[0052] Example 3: An antibacterial and disinfectant gel for wounds, prepared from raw materials comprising the following components in parts by weight: 5 parts of PCA-HBPG, 8 parts of modified chitosan, 1.8 parts of 3-aminophenylboronic acid, 0.8 parts of iodine, 1.6 parts of potassium iodide, and 0.9 parts of curcumin.

[0053] The preparation method of PCA-HBPG comprises the following steps:

[0054] L1. Mix 0.1 mol of glycerol and 0.01 mol of sodium methoxide, stir at 30°C for 1 h, then heat to 60°C, evacuate, add 20 mL of 1,4-dioxane, and heat to 95°C. Add 2.1 mol of glycidol dropwise with stirring. After the addition is complete, react for 5 h. Remove the 1,4-dioxane by rotary evaporation. Dialyze the mixture against deionized water for 3 days and freeze-dry to obtain HBPG.

[0055] L2. Under a nitrogen atmosphere, add 1 g of protocatechuic acid and 2 g of HBPG obtained in step L1 to 100 mL of DMSO and stir to dissolve. Add 1.5 g of EDC·HCl and 0.16 g of DMAP. Incubate with stirring at 30°C in the dark for 24 h. Filter and dialyze the filtrate against DMSO for 48 h and then against deionized water for 72 h. Freeze-dry to obtain PCA-HBPG.

[0056] The preparation method of modified chitosan comprises the following steps:

[0057] V1. Dissolve 0.2 g of NaIO4 in 4 mL of deionized water to obtain a 5% w / v NaIO4 solution. Dissolve 0.3 g of asiatic acid in 150 mL of anhydrous ethanol. Add the NaIO4 solution dropwise. Adjust the pH to 3.5 with dilute hydrochloric acid. Stir and react at room temperature for 1 h. After the reaction, extract and wash repeatedly with ethyl acetate and saturated brine. Combine the organic phases, dry over anhydrous MgSO4, filter, and evaporate the solvent under reduced pressure.

[0058] V2. Add 1 g of chitosan to 50 mL of 1% v / v acetic acid solution and stir at 600 rpm for 2 h to obtain CS solution. Dissolve 0.1 g of the modified Centella asiatica acid obtained in step V1 in 50 mL of ethanol. Add 0.05 g of EDC·HCl and 0.03 g of NHS, adjust the pH to 5, and stir for 2 h. Mix with the CS solution and react with stirring for 24 h. Dialyze in deionized water for 3 days, freeze-dry, wash with anhydrous ethanol, and dry in vacuo to obtain modified chitosan.

[0059] This embodiment also provides a method for preparing the wound antibacterial disinfection gel, comprising the following steps:

[0060] 0.8 g of iodine and 1.6 g of potassium iodide were added to 40 mL of deionized water and mixed to obtain iodine solution. 8 g of modified chitosan was dissolved in 400 mL of 1% v / v acetic acid solution to obtain a modified chitosan solution. 1.8 g of 3-aminophenylboronic acid was added to 360 mL of PBS solution with a pH of 7.4 and dissolved by ultrasonication. 5 g of PCA-HBPG was added, and the mixture was heated in a water bath at 60°C and stirred at 600 rpm for 1 h. 90 mL of a 10 mg / mL ethanol solution of curcumin was added to the modified chitosan solution and stirred evenly. The pH value was adjusted to 7.4, and the mixture was stirred at 300 rpm at room temperature for 1 h. The mixture was allowed to stand for 6 h, and iodine solution was added. The mixture was allowed to stand at 4°C for 12 h. The ethanol was removed under reduced pressure, and the mixture was washed with deionized water. The surface moisture was absorbed with filter paper to obtain an antibacterial and disinfectant gel for wounds.

[0061] The only difference between Comparative Example 1 and Example 1 is that HBPG is used instead of PCA-HBPG.

[0062] The only difference between Comparative Example 2 and Example 1 is that chitosan is used instead of modified chitosan.

[0063] Experimental Example 1: The revived Staphylococcus aureus was diluted with LB medium to a concentration of 10 4 CFU / mL, and a bacterial suspension was obtained. The gel samples prepared in Example 1-3 and Comparative Example 1-2 were sterilized by ultraviolet light and added to the bacterial suspension at a ratio of 50 mg / mL as the experimental group. The pure cell suspension was used as the control group and cultured in a constant temperature incubator at 37°C for 12 h. The bacterial solution was diluted and spread on an agar medium, cultured overnight at 37°C and counted, and the inhibition rate was calculated. The inhibition rate = (the number of colonies in the control group - the number of colonies in the experimental group) / the number of colonies in the control group. The results are shown in FIG. Figure 1 shown.

[0064] Figure 1 The results showed that the antibacterial rates of Examples 1-3 were significantly better than those of Comparative Examples 1-2. In Comparative Example 1, HBPG was used instead of PCA-HBPG, i.e., PCA was not grafted, and the antibacterial activity decreased; in Comparative Example 2, chitosan was not modified, and the antibacterial activity decreased. The above results indicate that the wound antibacterial disinfection gel prepared by the present invention has a good antibacterial effect and can preferentially inhibit wound infection.

[0065] Experimental Example 2: The wound antibacterial disinfection gel prepared in Example 1 and Comparative Examples 1-2 was placed at room temperature, and samples were taken at 1, 3, 5, 7 and 9 months to determine the antibacterial rate. The results are as follows: Figure 2 shown.

[0066] Figure 2 The results showed that after 9 months, the gel sample of Example 1 still had a good antibacterial effect, while the antibacterial effect of Comparative Examples 1-3 decreased significantly, indicating that the wound antibacterial disinfection gel prepared by the present invention has good stability and long-term antibacterial effect.

[0067] Experimental Example 3: 27 SPF female mice were selected and randomly divided into 5 groups, with 9 mice in each group. The mice were anesthetized with 4% chloral hydrate intraperitoneally. The hair on the back of the mice was shaved and a circular wound with a diameter of 8 mm was made. 300 μL of the gel of Example 1 of the present invention and Comparative Examples 1-2 was applied to the wound surface, and the gel was changed every 2 days. The wound healing was observed on the 3rd, 7th, 10th and 15th days after surgery, the wound area was measured, and the wound healing rate was calculated. The results are shown in FIG. Figure 3 shown.

[0068] Figure 3 The results showed that the wound healing rate of Example 1 was better than that of Comparative Examples 1-2. In Comparative Example 1, HBPG was used instead of PCA-HBPG, and the cross-linking degree decreased, and the wound healing effect decreased. In Comparative Example 2, the chitosan was not modified, the cross-linking degree decreased, the effective ingredient loading effect decreased, and the wound healing effect decreased, indicating that the wound antibacterial disinfection gel of the present invention can effectively disinfect and sterilize and promote wound healing.

[0069] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention is limited to these examples. Within the scope of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

Claims

1. An antibacterial disinfection gel for wounds, characterized in that: The preparation raw materials include the following components in parts by weight: PCA-HBPG 4-6 parts, modified chitosan 6-9 parts, 3-aminophenylboronic acid 1.5-2 parts, iodine 0.6-0.9 parts, potassium iodide 1.2-1.8 parts, curcumin 0.8-1 part; The preparation method of PCA-HBPG comprises the following steps: L1. Mix glycerol and sodium methoxide, stir and react at 30°C. Heat to 60°C, evacuate, add 1,4-dioxane, and heat to 95°C. Add glycidol dropwise with stirring. After the addition is complete, remove 1,4-dioxane by rotary evaporation, dialyze, and freeze-dry to obtain HBPG. L2. Under a nitrogen atmosphere, protocatechuic acid and the HBPG obtained in step L1 were added to DMSO and stirred to dissolve. EDC·HCl and DMAP were added and stirred at 30°C in the dark for 24 h. The mixture was filtered, dialyzed, and freeze-dried to obtain PCA-HBPG. The preparation method of the modified chitosan comprises the following steps: V1. NaIO4 was dissolved in deionized water to obtain a 5% w / v NaIO4 solution. Centella asiatica was dissolved in anhydrous ethanol, and the NaIO4 solution was added dropwise. The reaction was stirred and the mixture was repeatedly extracted and washed with ethyl acetate and saturated brine. The organic phases were combined, dried over anhydrous MgSO4, filtered, and the solvent was evaporated under reduced pressure. V2. Add chitosan to a 1% v / v acetic acid solution and stir to obtain a CS solution. Dissolve the modified asiatic acid obtained in step V1 in ethanol. Add EDC·HCl and NHS, adjust the pH to 5, and stir. Mix the mixture with the CS solution. Stir and react for 24 h. Dialyze, freeze-dry, wash, and vacuum dry to obtain modified chitosan.

2. The wound antibacterial disinfection gel according to claim 1, characterized in that: In step L1, the molar ratio of the glycerol, sodium methoxide and glycidol is 1:0.1:21; the molar ratio of glycerol to 1,4-dioxane is 0.1 mol:20 mL.

3. The wound antibacterial disinfection gel according to claim 2, characterized in that: In step L2, the mass concentration of protocatechuic acid in DMSO is 10 mg / mL; the mass ratio of protocatechuic acid, HBPG, EDC·HCl and DMAP is 1:2:1.5:0.

16.

4. The wound antibacterial disinfection gel according to claim 3, characterized in that: In step V1, the mass concentration of asiatic acid in anhydrous ethanol is 2 mg / mL; the mass ratio of asiatic acid to NaIO4 is 3:

2.

5. The wound antibacterial disinfection gel according to claim 4, characterized in that: In step V2, the mass concentration of the chitosan in the 1% v / v acetic acid solution is 20 mg / mL; the mass ratio of modified asiatic acid to chitosan is 1:10; and the mass ratio of modified asiatic acid, EDC·HCl, and NHS is 1:0.5:0.

3.

6. A method for preparing the wound antibacterial disinfection gel according to any one of claims 1 to 5, characterized in that: The following steps are involved: Iodine and potassium iodide are added to deionized water to obtain iodine solution, modified chitosan is dissolved in acetic acid solution to obtain modified chitosan solution, 3-aminophenylboronic acid is added to PBS solution to dissolve, PCA-HBPG is added, heated and stirred, curcumin and modified chitosan solution are added, stirred, allowed to stand, and iodine solution is added to obtain an antibacterial disinfection gel for wound surface.

7. The method for preparing the wound antibacterial disinfection gel according to claim 6, characterized in that: The mass concentration of potassium iodide in deionized water is 40 mg / mL; the mass concentration of 3-aminophenylboronic acid in PBS solution with pH=7.4 is 5 mg / mL.

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