Method for promoting citral-type camphor tree clumpy bud proliferation

Abstract

The invention discloses a method for promoting citral-type camphor tree clump bud proliferation, which comprises the following steps: selecting a stock plant which is free from plant diseases and insect pests and grows robustly, hanging a tag, and spraying cytokinin on leaf surfaces at set intervals from March; current-year semi-lignified branches are collected at the beginning of June; the stem segments are subjected to pruning and bacteriostatic soaking; performing multiplication culture, observing the growth condition of the cluster buds, and counting the multiplication coefficient and average plant height of the adventitious buds. According to the method, a large number of effective cluster buds are obtained in a short time through technologies such as stem segment bacteriostasis and germination promotion, culture medium and culture condition optimization and the like, the effective propagation coefficient of citral type camphor trees is increased, technical support is provided for conservation and propagation of tree species, the seedling cultivation period is shortened, the seedling cultivation generations are reduced, the seedling quality is guaranteed, and the method is suitable for large-scale popularization and application. And the waste of manpower and material resources is reduced, so that the healthy and sustainable development of citral camphor tree and essence and perfume industries is promoted.

Description

Technical Field

[0001] The invention relates to the field of plant bud proliferation, and in particular to a method for promoting the proliferation of citral-type camphor tree buds. Background Art

[0002] Natural citral is an important component of plant essential oils, widely used in medicine, food, and chemical industries. It possesses significant economic value and development potential, generating significant market demand. Among woody plants, citral is found in the fruit of Litsea cubeba. Citral-producing camphor tree leaf essential oils, containing up to 80% citral, are currently the primary source of natural citral. However, citral-producing camphor trees are currently scarce, creating a weak resource base for industrial development. There is an urgent need to increase resource availability through asexual propagation to address the current citral shortage.

[0003] Therefore, it is an urgent problem for those skilled in the art to provide a method for promoting the induction of citral-type camphor tree tissue culture cluster buds, which can improve the resource base and quality of citral-type camphor trees, improve the current low proliferation rate of cutting and tissue culture technology, and thus promote the healthy and sustainable development of the natural citral industry. Summary of the Invention

[0004] In view of this, the present invention provides a method for promoting the proliferation of citral-type camphor tree buds.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0006] A method for promoting the proliferation of citral-type camphor tree buds comprises the following steps:

[0007] Step 1: Select healthy and healthy plants without diseases and insect pests as mother plants and list them. Starting in March, spray cytokinins on the leaves at regular intervals until the end of May.

[0008] Step 2: Beginning in early June, collect semi-lignified branches of the mother plant at noon on a sunny day and store and transport them in foam boxes filled with ice packs;

[0009] Step 3: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0010] Step 4: Start the culture and cultivate sterile sprouts;

[0011] Step 5: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0012] Preferably, in step 1, cytokinin 6-BA is sprayed on the leaves once every 15 days, and the concentration of cytokinin 6-BA is 1.5 mg / L.

[0013] Preferably, in step 3, each stem segment is 10 cm long, and after being brushed in the soapberry extract, the soaking time is 30 minutes, the rinsing time is 3 hours, the concentration of the aspirin solution is 0.1 mg / ml, and the soaking time is 30 minutes.

[0014] Preferably, in step 3, the preparation method of the sapodilla extract is:

[0015] After the soap nuts are dried, they are crushed with a grinder. 100g of soap nut powder is added to 500ml of 50°C hot water each time, and extracted for 30 minutes under ultrasonic power of 600W. After that, the mixture is shaken thoroughly and placed in a centrifuge. Centrifuge at 1200rpm for 5min and the supernatant is taken to obtain the soap nut extract.

[0016] Preferably, in step 4, the initiation culture is initiated by using MS+2.00 mg / L 6-BA+0.05 mg / L 1 BA+6.00 g / L agar+30.00 g / L sucrose as the initiation culture medium.

[0017] Preferably, in step 5, the proliferation culture is based on MS as the basal medium, supplemented with 30 g / L sucrose and 6 g / L agar, and randomly added with coconut milk in different volume ratios.

[0018] Preferably, the coconut milk is the natural juice from purchased fresh coconuts.

[0019] Preferably, in step 6, SA, 6-BA, NAA, and KT are selected as the hormone combination for adventitious bud proliferation, and the hormone concentrations are set to SA 1 mg / L, 6-BA concentrations are respectively: 0.5 mg / L, 1 mg / L, and 2 mg / L, NAA concentrations are respectively: 0.05 mg / L, 0.1 mg / L, and 0.2 mg / L, and KT concentrations are respectively 0.05 mg / L, 0.1 mg / L, and 0.2 mg / L. The growth of the clustered buds is observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds are counted for 30 days to compare the clustered bud proliferation effects of the citral-type camphor trees of each scheme.

[0020] Compared with the prior art, the present invention has achieved the following technical effects:

[0021] The present invention can obtain a large number of effective clustered buds in a relatively short period of time, thereby improving the effective reproduction coefficient of the citral-type camphor tree, providing technical support for the conservation and propagation of the tree species, and also shortening the cultivation cycle of the seedlings, reducing the number of seedling cultivation generations, ensuring the quality of the seedlings, reducing the waste of manpower and material resources, and further promoting the healthy and sustainable development of the citral-type camphor tree and flavor and fragrance industries. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a diagram of the bud clusters of citral-type camphor trees propagated on different culture media of the present invention. DETAILED DESCRIPTION

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0024] Example 1:

[0025] The present invention discloses a method for promoting the proliferation of citral-type camphor tree buds, comprising the following steps:

[0026] Step 1: Select healthy and healthy plants without diseases and insect pests as mother plants and list them. Starting in March, spray cytokinins on the leaves at regular intervals until the end of May.

[0027] Step 2: Beginning in early June, collect semi-lignified branches of the mother plant at noon on a sunny day and store and transport them in foam boxes filled with ice packs;

[0028] Step 3: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0029] Step 4: Start the culture and cultivate sterile sprouts;

[0030] Step 5: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0031] In step 1, cytokinin 6-BA was sprayed on the leaves once every 15 days, and the concentration of cytokinin 6-BA was 1.5 mg / L.

[0032] In step 3, each stem segment of 10 cm is brushed in the saponin extract and then soaked for 30 minutes, rinsed for 3 hours, and the concentration of the aspirin solution is 0.1 mg / ml, and the soaking time is 30 minutes.

[0033] In step 3, the preparation method of the sapodilla extract is:

[0034] After the soap nuts are dried, they are crushed with a grinder. 100g of soap nut powder is added to 500ml of 50°C hot water each time, and extracted for 30 minutes under ultrasonic power of 600W. After that, the mixture is shaken thoroughly and placed in a centrifuge. Centrifuge at 1200rpm for 5min and the supernatant is taken to obtain the soap nut extract.

[0035] In step 4, MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose was used as the starting medium for the initiation culture.

[0036] In step 5, the proliferation culture is based on MS medium, supplemented with 30 g / L sucrose, 6 g / L agar, and coconut milk at a volume ratio of 1:4.

[0037] Coconut milk is the natural juice from freshly purchased coconuts.

[0038] In step 5, SA, 6-BA, NAA, and KT were selected as the hormone combination for adventitious bud proliferation. The hormone concentrations were set to 1 mg / L for SA, 2 mg / L for 6-BA, 0.2 mg / L for NAA, and 0.05 mg / L for KT. The growth of the clustered buds was observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds were calculated after 30 days to compare the clustered bud proliferation effects of citral-type camphor trees under each scheme.

[0039] In step 5, the culture conditions for proliferation culture are: blue light with a wavelength of 450-500 nm is used for 15 days at the initial stage of culture, and then switched to light culture with a light-dark ratio of 12h:12h and a light intensity of 2000-2400lx; the temperature inside the culture chamber is between 22-24°C.

[0040] Comparative Example 1:

[0041] The present invention discloses a method for promoting the proliferation of citral-type camphor tree buds, comprising the following steps:

[0042] Step 1: Collect the semi-lignified branches of the mother plant at noon on a sunny day in early June and store and transport them in a foam box filled with ice packs;

[0043] Step 2: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0044] Step 3: Start the culture and cultivate sterile sprouts;

[0045] Step 4: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0046] In step 3, MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose was used as the starting medium for the initiation culture.

[0047] In step 4, the proliferation culture is based on MS medium, supplemented with 30 g / L sucrose and 6 g / L agar.

[0048] In step 4, SA, 6-BA, NAA, and KT were selected as the hormone combination for adventitious bud proliferation. The hormone concentrations were set to 1 mg / L for SA, 0.5 mg / L for 6-BA, 0.1 mg / L for NAA, and 0.2 mg / L for KT. The growth of the clustered buds was observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds were calculated after 30 days. The clustered bud proliferation effects of each scheme on citral-type camphor trees were compared.

[0049] In step 4, the culture conditions for proliferation culture are: light-dark ratio of 12h:12h, light intensity of 2000-2400lx, and the temperature inside the culture chamber is between 22-24°C.

[0050] Comparative Example 2:

[0051] The present invention discloses a method for promoting the proliferation of citral-type camphor tree buds, comprising the following steps:

[0052] Step 1: Select healthy and healthy plants without diseases and insect pests as mother plants and list them. Starting in March, spray cytokinins on the leaves at regular intervals until the end of May.

[0053] Step 2: Beginning in early June, collect semi-lignified branches of the mother plant at noon on a sunny day and store and transport them in foam boxes filled with ice packs;

[0054] Step 3: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0055] Step 4: Start the culture and cultivate sterile sprouts;

[0056] Step 5: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0057] In step 1, cytokinin 6-BA was sprayed on the leaves once every 15 days, and the concentration of cytokinin 6-BA was 1.5 mg / L.

[0058] In step 3, each stem segment of 10 cm is brushed in the saponin extract and then soaked for 30 minutes, rinsed for 3 hours, and the concentration of the aspirin solution is 0.1 mg / ml, and the soaking time is 30 minutes.

[0059] In step 3, the preparation method of the sapodilla extract is:

[0060] After the soap nuts are dried, they are crushed with a grinder. 100g of soap nut powder is added to 500ml of 50°C hot water each time, and extracted for 30 minutes under ultrasonic power of 600W. After that, the mixture is shaken thoroughly and placed in a centrifuge. Centrifuge at 1200rpm for 5min and the supernatant is taken to obtain the soap nut extract.

[0061] In step 4, MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose was used as the starting medium for the initiation culture.

[0062] In step 5, the proliferation culture is based on MS medium, supplemented with 30 g / L sucrose, 6 g / L agar, and coconut milk at a volume ratio of 1:9.

[0063] Coconut milk is the natural juice from freshly purchased coconuts.

[0064] In step 5, SA, 6-BA, NAA, and KT were selected as the hormone combination for adventitious bud proliferation. The hormone concentrations were set to 1 mg / L for SA, 2 mg / L for 6-BA, 0.05 mg / L for NAA, and 0.2 mg / L for KT. The growth of the clustered buds was observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds were calculated after 30 days to compare the clustered bud proliferation effects of citral-type camphor trees under each scheme.

[0065] In step 5, the culture conditions for proliferation culture are: blue light with a wavelength of 450-500 nm is used for 15 days at the initial stage of culture, and then switched to light culture with a light-dark ratio of 12h:12h and a light intensity of 2000-2400lx; the temperature inside the culture chamber is between 22-24°C.

[0066] Comparative Example 3:

[0067] The present invention discloses a method for promoting the proliferation of citral-type camphor tree buds, comprising the following steps:

[0068] Step 1: Collect the semi-lignified branches of the mother plant at noon on a sunny day in early June and store and transport them in a foam box filled with ice packs;

[0069] Step 2: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0070] Step 3: Start the culture and cultivate sterile sprouts;

[0071] Step 4: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0072] In step 3, MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose was used as the starting medium for the initiation culture.

[0073] In step 4, the proliferation culture is based on MS medium, supplemented with 30 g / L sucrose and 6 g / L agar.

[0074] In step 4, SA, 6-BA, NAA, and KT were selected as the hormone combination for adventitious bud proliferation. The hormone concentrations were set to 1 mg / L for SA, 0.5 mg / L for 6-BA, 0.2 mg / L for NAA, and 0.1 mg / L for KT. The growth of the clustered buds was observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds were calculated after 30 days to compare the clustered bud proliferation effects of citral-type camphor trees under each scheme.

[0075] In step 4, the culture conditions for proliferation culture are: light-dark ratio of 12h:12h, light intensity of 2000-2400lx, and the temperature inside the culture chamber is between 22-24°C.

[0076] Comparative Example 4:

[0077] The present invention discloses a method for promoting the proliferation of citral-type camphor tree buds, comprising the following steps:

[0078] Step 1: Collect the semi-lignified branches of the mother plant at noon on a sunny day in early June and store and transport them in a foam box filled with ice packs;

[0079] Step 2: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0080] Step 3: Start the culture and cultivate sterile sprouts;

[0081] Step 4: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0082] In step 3, MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose was used as the starting medium for the initiation culture.

[0083] In step 4, the proliferation culture is based on MS medium, supplemented with 30 g / L sucrose, 6 g / L agar, and coconut milk at a volume ratio of 1:4.

[0084] Coconut milk is the natural juice from freshly purchased coconuts.

[0085] In step 4, SA, 6-BA, NAA, and KT were selected as the hormone combination for adventitious bud proliferation. The hormone concentrations were set to 1 mg / L for SA, 1.0 mg / L for 6-BA, 0.2 mg / L for NAA, and 0.2 mg / L for KT. The growth of the clustered buds was observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds were calculated after 30 days to compare the clustered bud proliferation effects of citral-type camphor trees under each scheme.

[0086] In step 4, the culture conditions for proliferation culture are: light-dark ratio of 12h:12h, light intensity of 2000-2400lx, and the temperature inside the culture chamber is between 22-24°C.

[0087] Comparative Example 5:

[0088] The present invention discloses a method for promoting the proliferation of citral-type camphor tree buds, comprising the following steps:

[0089] Step 1: Select healthy and healthy plants without diseases and insect pests as mother plants and list them. Starting in March, spray cytokinins on the leaves at regular intervals until the end of May.

[0090] Step 2: Beginning in early June, collect semi-lignified branches of the mother plant at noon on a sunny day and store and transport them in foam boxes filled with ice packs;

[0091] Step 3: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use.

[0092] Step 4: Start the culture and cultivate sterile sprouts;

[0093] Step 5: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

[0094] In step 1, cytokinin 6-BA was sprayed on the leaves once every 15 days, and the concentration of cytokinin 6-BA was 1.5 mg / L.

[0095] In step 3, each stem segment of 10 cm is brushed in the saponin extract and then soaked for 30 minutes, rinsed for 3 hours, and the concentration of the aspirin solution is 0.1 mg / ml, and the soaking time is 30 minutes.

[0096] In step 3, the preparation method of the sapodilla extract is:

[0097] After the soap nuts are dried, they are crushed with a grinder. 100g of soap nut powder is added to 500ml of 50°C hot water each time, and extracted for 30 minutes under ultrasonic power of 600W. After that, the mixture is shaken thoroughly and placed in a centrifuge. Centrifuge at 1200rpm for 5min and the supernatant is taken to obtain the soap nut extract.

[0098] In step 4, MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose was used as the starting medium for the initiation culture.

[0099] In step 5, the proliferation culture is based on MS medium, supplemented with 30 g / L sucrose, 6 g / L agar, and coconut milk at a volume ratio of 1:9.

[0100] Coconut milk is the natural juice from freshly purchased coconuts.

[0101] In step 5, SA, 6-BA, NAA, and KT were selected as the hormone combination for adventitious bud proliferation. The hormone concentrations were set to 1 mg / L for SA, 1 mg / L for 6-BA, 0.1 mg / L for NAA, and 0.05 mg / L for KT. The growth of the clustered buds was observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds were calculated after 30 days to compare the clustered bud proliferation effects of citral-type camphor trees under each scheme.

[0102] In step 5, the culture conditions for proliferation culture are: blue light with a wavelength of 450-500 nm is used for 15 days at the initial stage of culture, and then switched to light culture with a light-dark ratio of 12h:12h and a light intensity of 2000-2400lx; the temperature inside the culture chamber is between 22-24°C.

[0103] According to the above Example 1 and Comparative Examples 1-5 of the present invention, the culture medium of the present invention (20 ml / L coconut milk + 1 mg / LSA + 2.00 mg / L 6-BA + 0.20 mg / L NAA + 0.05 mg / L KT) has the highest adventitious bud proliferation coefficient of 8.16, and the average plant height is 2.73 cm, which are significantly higher than the other 5 groups. At the same time, the plants on the culture medium of the present invention have the best growth, the number of effective adventitious buds is large, and the leaves are green, as shown in Table 1 and Figure 1 shown.

[0104] Table 1: Citral-type camphor tree bud proliferation on different culture media

[0105]

[0106] The above description is merely a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any minor modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A method for promoting the proliferation of citral-type camphor tree buds, characterized in that: The steps include: Step 1: Select healthy and healthy plants without diseases and insect pests as mother plants and list them. Starting in March, spray cytokinins on the leaves at regular intervals until the end of May. Step 2: Beginning in early June, collect semi-lignified branches of the mother plant at noon on a sunny day and store and transport them in foam boxes filled with ice packs; Step 3: After returning to the laboratory, the stem segments are trimmed, the leaves are cut off, and the petioles are retained. The segments are then brushed and soaked in saponin extract, rinsed under running water, and soaked in aspirin solution. The rinsed segments are then transferred to a sterile operating table for use. Step 4: Start the culture and cultivate sterile sprouts; Step 5: Carry out proliferation culture, add hormones for adventitious bud proliferation, observe the growth of clustered buds during the proliferation culture process, and calculate the proliferation coefficient and average plant height of the adventitious buds.

2. A method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that, In the step 1, cytokinin 6-BA is sprayed on the leaves once every 15 days, and the concentration of cytokinin 6-BA is 1.5 mg / L.

3. A method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that, In step 3, each stem segment of 10 cm is brushed in the soapberry extract and then soaked for 30 minutes, rinsed for 3 hours, and the concentration of the aspirin solution is 0.1 mg / ml, and the soaking time is 30 minutes.

4. A method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that, In step 3, the preparation method of the soapberry extract is: After the soap nuts are dried, they are crushed with a grinder. 100g of soap nut powder is added to 500ml of 50°C hot water each time, and extracted for 30 minutes under ultrasonic power of 600W. After that, the mixture is shaken thoroughly and placed in a centrifuge. Centrifuge at 1200rpm for 5min and the supernatant is taken to obtain the soap nut extract.

5. A method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that: In step 4, the initiation culture was started by using MS + 2.00 mg / L 6-BA + 0.05 mg / L IBA + 6.00 g / L agar + 30.00 g / L sucrose as the initiation culture medium.

6. The method for promoting the proliferation of citral-type camphor tree buds according to claim 1, wherein: In step 4, the culture conditions for initiating the culture are light culture with a light-dark ratio of 12h:12h, a light intensity of 2000-2400lx, and a culture temperature between 22-24°C.

7. The method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that: In step 5, the proliferation culture is based on MS medium, sucrose 30g / L, agar 6g / L, and coconut milk in different volume ratios is randomly added.

8. The method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that: In step 5, the culture conditions for proliferation culture are: blue light with a wavelength of 450-500 nm is used for 15 days in the initial culture period, and then light culture is switched to a light-dark ratio of 12h:12h and a light intensity of 2000-2400lx; the temperature inside the culture chamber is between 22-24°C.

9. The method for promoting the proliferation of citral-type camphor tree buds according to claim 7, characterized in that: The coconut milk is the natural juice in purchased fresh coconuts.

10. The method for promoting the proliferation of citral-type camphor tree buds according to claim 1, characterized in that: In step 5, SA, 6-BA, NAA, and KT are selected as the hormone combination for adventitious bud proliferation, and the hormone concentrations are set to SA 1 mg / L, 6-BA concentrations are respectively: 0.5 mg / L, 1 mg / L, and 2 mg / L, NAA concentrations are respectively: 0.05 mg / L, 0.1 mg / L, and 0.2 mg / L, and KT concentrations are respectively 0.05 mg / L, 0.1 mg / L, and 0.2 mg / L. The growth of the clustered buds is observed during the proliferation culture process, and the proliferation coefficient and average plant height of the adventitious buds are counted for 30 days to compare the clustered bud proliferation effects of the citral-type camphor trees of each scheme.

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