Traditional Chinese medicine extract for treating aflatoxin poisoning disease of ducklings as well as preparation method and application of traditional Chinese medicine extract

Abstract

The invention discloses a traditional Chinese medicine extract for treating aflatoxin poisoning disease of ducklings and a preparation method and application of the traditional Chinese medicine extract, and relates to the field of traditional Chinese veterinary medicines, the traditional Chinese medicine extract is a radix paeoniae alba tannin extract, the radix paeoniae alba tannin extract comprises the following components: 62-63% of condensed tannin and 37-38% of hydrolyzed tannin; the high-purity tannin substances are extracted from radix paeoniae alba decoction pieces through a specific technology of'cold soaking-gelatin precipitation '. The traditional Chinese medicine extract provided by the invention can effectively relieve the toxic effect of aflatoxin on ducklings under the condition of low dosage, obviously relieve liver injury and improve liver function indexes, so that normal growth and development of the ducklings are guaranteed, and the survival rate and the production performance are improved.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese veterinary medicine, in particular to a traditional Chinese medicine extract for treating aflatoxin poisoning in ducklings, and a preparation method and application thereof. Background Art

[0002] Currently, the main methods for detoxification of aflatoxins include physical detoxification, chemical detoxification, biological detoxification and nutritional intervention. However, the physical detoxification method may destroy the nutrients; the chemical detoxification method is costly and may leave chemical residues; the biological detoxification method is not widely used, while the nutritional intervention method is highly safe, has few side effects and is widely available.

[0003] As a traditional Chinese medicinal material, white peony root has the effects of nourishing liver blood, astringing liver yin, and calming liver yang, and has a protective effect on the liver. Studies have shown that white peony root has a therapeutic effect on liver diseases such as cholestatic liver fibrosis, acute liver injury, and non-alcoholic fatty liver disease.

[0004] The toxicity of aflatoxins varies significantly across species. Ducklings are highly susceptible to aflatoxins, with a median lethal dose significantly lower than that of other animals. Existing research suggests that while crude white peony extract can mitigate the toxicity of aflatoxin B1 (AFB1), its clinical application is severely limited by its complex composition, unclear target, potential adverse reactions at high doses, and inability to achieve effective detoxification at low doses. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a traditional Chinese medicine extract for treating aflatoxin poisoning in ducklings, as well as its preparation method and application. The traditional Chinese medicine extract can effectively alleviate the toxic effects of aflatoxin on ducklings under low-dose conditions, significantly reduce liver damage, and improve liver function indicators, thereby ensuring the normal growth and development of ducklings and improving survival rate and production performance.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is: The present invention provides a traditional Chinese medicine extract for treating aflatoxin poisoning in ducklings. The traditional Chinese medicine extract is a white peony tannin extract. The white peony tannin extract comprises condensed tannins and hydrolyzed tannins. The condensed tannins account for 62% to 63%, and the hydrolyzed tannins account for 37% to 38%.

[0007] As a preferred embodiment, the condensed tannin includes proanthocyanidin trimers and dimers, and the hydrolyzed tannin includes 1,2,3,4,6-O-pentagalloylglucose.

[0008] As a preferred embodiment, the condensed tannins in the white peony tannin extract account for 62.83% and the hydrolyzed tannins account for 37.17%.

[0009] The invention relates to an application of the white peony tannin extract in treating aflatoxin poisoning in ducklings.

[0010] The present invention also provides a method for preparing a traditional Chinese medicine extract for treating aflatoxin poisoning in ducklings, comprising the following steps: S1, crush and sieve the white peony root slices, add 50% acetone aqueous solution at a material-liquid ratio of 1:8, and cold soak for 7 days, stirring several times a day; S2, after 7 days, filter and concentrate the filtrate under reduced pressure to recover acetone; S3, add 1% hot gelatin solution to precipitate, let stand, centrifuge, and discard the supernatant; S4, dissolving the precipitate in 50% acetone solution, heating and stirring, cooling to room temperature, centrifuging, and discarding the precipitate; S5, the supernatant was concentrated under reduced pressure and freeze-dried, and the obtained product was purified by Sephadex LH-20, eluted with 50% methanol and 70% acetone in sequence, and the fractions were combined and concentrated to obtain the white peony tannin extract.

[0011] As a preferred embodiment, in S1, the powder particle size of the white peony root slices after crushing is 200 mesh.

[0012] As a preferred solution, in S3, the standing time is 15 minutes.

[0013] As a preferred solution, in S4, the heating temperature is 50°C.

[0014] According to the above technical solution, the beneficial effects of the present invention are: The white peony tannin extract and its preparation method provided by the present invention offer significant advantages. Extracting tannins from the traditional Chinese medicinal plant white peony effectively mitigates the toxic effects of AFB1 in ducklings. Experimental results demonstrate that low-dose white peony tannin extracts have a significant therapeutic effect on AFB1-induced liver damage in ducklings, as measured by biochemical and antioxidant indicators in serum and liver tissue, as well as pathological changes in liver tissue. They demonstrate excellent detoxification effects by significantly improving liver function indicators, restoring antioxidant enzyme activity, and alleviating liver pathological damage. The purified tannin components of the present invention avoid the adverse reactions of traditional crude extracts, achieving significant therapeutic effects at low doses. Used alone, they are safe and do not cause liver damage. Using a specific "cold soaking-gelatin precipitation" process, high-purity tannins, comprising 62% to 63% condensed tannins and 37% to 38% hydrolyzed tannins, are extracted from white peony. This invention successfully addresses the key issue of balancing dose and effect in existing technologies, demonstrating that tannins in white peony are the primary chemical components for treating aflatoxin poisoning in ducklings. The preparation method provided by the present invention is simple and efficient to operate, and the components of the obtained extract are clear and controllable, with high yield and purity. It provides a traditional Chinese medicine chemical component that is highly safe, has few side effects, is widely available, is beneficial to the body, and can alleviate the toxic effects of aflatoxin on the body. It provides a natural detoxification solution for aflatoxin poisoning and a reliable means for preventing and treating aflatoxin poisoning in ducklings. It has broad application prospects in the field of animal husbandry. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Figure 2 is the change of serum CAT activity, SOD activity and MDA content in ducklings under different treatment conditions. # indicates P < 0.05 compared with the control group, ## indicates P < 0.01 compared with the control group; ** indicates P < 0.01 compared with the AFB1 group; Figure 2 The graph shows the changes in CAT activity, SOD activity, MDA content and GSH-Px activity in the liver of ducklings under different treatment conditions. # indicates P < 0.05 compared with the control group, ## indicates P < 0.01 compared with the control group; * indicates P < 0.05 compared with the AFB1 group, ** indicates P < 0.01 compared with the AFB1 group; Figure 3 HE staining of the liver tissue pathomorphological changes under different treatment conditions; Figure 4 The MASSON staining diagram of the pathomorphological changes of liver tissue under different treatment conditions; Figure 5 Oil red O staining of liver tissue pathomorphological changes under different treatment conditions; Figure 6Figure 2 shows the pathomorphological changes of liver cells under different treatment conditions; Figure 7 13C NMR spectrum of white peony tannin extract; Figure 8 This is the two-dimensional nuclear magnetic resonance spectrum (HSQC spectrum) of white peony tannin extract. DETAILED DESCRIPTION

[0016] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0017] In the following examples, unless otherwise specified, all reagents and materials used are commercially available.

[0018] 1. Experimental Materials Sixty one-day-old Mallard ducks were purchased from a certain hatchery; white peony root slices were purchased from Zhang Zhongjing Pharmacy; aflatoxin B1 was purchased from Hubei Benuofu Chemical Technology Co., Ltd.; biochemical, antioxidant and other reagents were purchased from Nanjing Jiancheng Bioengineering Institute; Smart SD1 fully automatic dry biochemical analyzer was produced by Chengdu Smart Technology Co., Ltd.; BioTek Epoch full-wavelength microplate reader was purchased from BioTek Company in the United States.

[0019] 2. Experimental Methods 2.1 Preparation of white peony tannin extract White peony root slices were pulverized and passed through a 200-mesh standard sieve. Eight volumes of 50% acetone aqueous solution were added at a material-to-liquid ratio of 1:8 and cold-soaked for 7 days, stirring several times daily. After 7 days, the filtrate was filtered, and the filtrate was concentrated under reduced pressure on a rotary evaporator to recover the acetone. The resulting solution was added with a 1% hot gelatin solution and precipitated until no precipitate formed. The solution was allowed to stand for 15 minutes, centrifuged, and the supernatant discarded. The resulting precipitate was dissolved in an appropriate amount of 50% acetone solution, heated at 50°C with stirring, cooled, centrifuged, and discarded. The supernatant was concentrated under reduced pressure on a rotary evaporator, and the concentrate was freeze-dried. The resulting product was purified on Sephadex LH-20, eluted sequentially with 50% methanol and 70% acetone, and the combined fractions were concentrated to obtain the white peony tannin extract.

[0020] 2.2 Component Analysis of White Peony Tannin Extracts The total phenolics in the tannin extracts of white peony root were determined using the Folin ciocaltEu reagent method. Folin ciocaltEu reagent (1N). Experimental steps: Take 0.5 mL of each standard solution, add 0.25 mL of Folin ciocaltEu reagent, and then add 1.25 mL of 20% sodium carbonate solution. Shake well, react for 40 minutes, and measure absorbance at 725 nm. Draw a standard curve based on the absorbance and concentration of the standard solutions. Take 5 μL of sample solution and add 495 μL of distilled water to obtain 0.5 mL of sample solution. Repeat the above steps, measure absorbance at 725 nm, and calculate the sample concentration and the tannic acid equivalent in the sample from the standard curve. The determination of tannic acid equivalents after removing tannins from total phenols was based on the principle of a PVPP-tannin binding assay. 1 g of PVPP was added to 1 mL of sample solution and 4 mL of distilled water. The mixture was vortexed, allowed to stand at 4°C for 15 minutes, vortexed again, and centrifuged at 3000 r / min for 10 minutes. The supernatant (1 mL) containing only simple phenols other than tannins was collected. The absorbance was measured according to the above procedure, and the tannic acid equivalents (TAEs) after removing tannins from total phenols were calculated using a standard curve. The final calculated tannin content per mg of white peony tannin extract was 0.68945873 mg of tannic acid equivalents.

[0021] Determination of condensed tannins (proanthocyanidins) in white peony tannin extracts: This method is based on the oxidative depolymerization of condensed tannins in a butanol-hydrochloric acid reagent. This experiment uses the n-butanol-hydrochloric acid method to determine the condensed tannin content in white peony tannins. Condensed tannin content is expressed in catechin equivalents. Sample and other reagent preparation: Sample solution: Dissolve 0.9 mg of condensed tannin sample in 6 mL of distilled water. Prepare the n-butanol-hydrochloric acid solution by combining 95 mL of n-butanol solution and 5 mL of hydrochloric acid solution. Experimental steps: Take 1 mL of sample solution or 1 mL of each standard solution, add 6 mL of n-butanol-hydrochloric acid solution, boil in a water bath for 75 minutes, cool, and measure absorbance at 550 nm. A standard curve is constructed based on the absorbance and concentration of the standard, and the sample concentration and catechin equivalents are calculated. The final calculated value is 0.433183828 mg of catechin equivalents per 1 mg of white peony tannin extract.

[0022] Determination of hydrolyzed tannin content in white peony tannin extracts: According to the above data, the tannin content in the sample is calculated: Tannin content = tannic acid equivalent (mg) - tannic acid equivalent (mg) after removing tannin from total phenols (1) The condensed tannin content is indirectly obtained based on the difference between the tannin content and the condensed tannin content: Hydrolyzed tannin content = tannin content (mg) - condensed tannin content (mg) (2) The hydrolyzed tannin content was indirectly determined based on the difference between the total phenol content and the condensed tannin content. The hydrolyzed tannin content was calculated using formula (2) as 0.256274902 mg. This indicates that condensed tannins accounted for 62.83% of the tannin extract from white peony root, while hydrolyzed tannins accounted for 37.17%.

[0023] Chemical component analysis of tannin extracts from white peony root: The tannin extracts from white peony root were purified by Sephadex LH-20 and eluted with 50% methanol and 70% acetone, respectively. The methanol fraction and acetone fraction were analyzed by liquid chromatography-mass spectrometry and nuclear magnetic resonance. Figure 7 and Figure 8 The results showed that the white peony tannin extract contained condensed tannins such as proanthocyanidin trimers and dimers, accounting for 62% of the total content; and high-polymerization galloylglucose hydrolyzed tannins, represented by 1,2,3,4,6-O-pentagalloylglucose (m / z 939.08), accounting for 37%. In addition, small amounts of other phenolic compounds such as gallic acid and paeoniflorin were detected.

[0024] 2.3 Animal Experiment Design Sixty healthy, one-day-old ducklings (average weight 35 ± 2g) were selected and co-housed until 7 days of age, receiving a basal diet and normal drinking water. At 8 days of age, the ducklings were randomly divided into four groups of 15 ducks each. The treatment settings for the four groups were as follows: ①Blank control group (Control, referred to as control group): the blank control group was given 2 mL of distilled water.

[0025] ②AFB1 model group (AFB1, referred to as AFB1 group), AFB1 group was treated with 490 μg·kg -1 · AFB1 of BW was dissolved in olive oil and administered orally.

[0026] ③ White Peony Tannin Control Group (Tannin, referred to as Tannin Group), Tannin Group was treated with 100 mg·kg -1 ·BW white peony tannins were dissolved in distilled water and administered orally.

[0027] ④AFB1-white peony tannin treatment group (AFB1+tannin, referred to as tannin treatment group), the tannin treatment group was treated with 490 μg·kg -1 AFB1 of BW was dissolved in olive oil and administered orally 30 minutes later at 100 mg·kg -1 ·BW white peony tannins were dissolved in distilled water and administered orally.

[0028] All groups were housed identically, with drug administration once daily for 14 days. Ducklings were caged, feces were cleaned daily, and the health and mortality of the ducklings were recorded. During the study, the ducklings had free access to water and food at room temperature. Ducklings were sacrificed on day 22 of age for sampling.

[0029] 2.4 Sample collection and processing Blood was collected from 22-day-old ducklings after fasting for 12 hours. The collected blood was allowed to stand for 2 hours, centrifuged, and the supernatant serum was collected and stored at -20°C for subsequent experiments. After blood collection, the ducklings were sacrificed, and fresh liver tissue was quickly collected and weighed. After weighing, liver tissue of appropriate size was fixed with 4% paraformaldehyde and electron microscopy fixative for preparation of histopathological sections. The remaining portion was quickly frozen in liquid nitrogen and then stored at -80°C for subsequent experiments.

[0030] 2.5 Determination of liver function biochemical and antioxidant indicators in liver and serum Serum index determination: A fully automatic biochemical analyzer was used to detect serum biochemical indicators: albumin (ALB), total protein (TP), globulin (GLOB), albumin / globulin ratio (A / G), total bilirubin (TB), total bile acids (TBA), total cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities, and the AST / ALT ratio was calculated.

[0031] Antioxidant marker assays: Frozen duckling liver samples were removed, added to an appropriate amount of physiological saline and grinding beads, and ground using a cryogenic grinder until no small tissue fragments remained. After grinding, the tissue homogenate was centrifuged. After centrifugation, the supernatant was aspirated, and serum samples were prepared for antioxidant marker assays. Subsequent steps were performed according to the instructions for the superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and glutathione peroxidase (GSH-PX) assay kits.

[0032] 2.6 Liver histopathological observation Liver tissue hematoxylin-eosin (HE) staining: Duckling liver tissue was rinsed in pre-chilled PBS, dried, and fixed in 4% paraformaldehyde for 48 h. The tissue was then dehydrated and paraffin-embedded. The solidified tissue block was removed from the embedding frame and trimmed with a blade. The trimmed block was fixed to the specimen holder of a microtome. Once the block was fully exposed, it was sectioned with a microtome to a thickness of 4 µm. The liver tissue sections were dried and stained with hematoxylin-eosin. After drying, a small amount of neutral gum was added to the sections, and the sections were mounted with coverslips, avoiding the introduction of air bubbles. After preparation, the sections were observed under an optical microscope for pathological changes, recorded, photographed, and analyzed.

[0033] Oil Red O staining of liver tissue: Add OCT embedding medium to a specimen holder, place the frozen tissue on the holder, and then add more OCT embedding medium. Place the holder on a quick-freeze block to allow the OCT to blanch and harden. Then, use a cryostat to slice the tissue to a thickness of approximately 4-6 µm. Remove the sections, warm them to room temperature, rinse them with distilled water to remove the embedding medium, and stain them with pre-prepared Oil Red O working solution. After staining, differentiate the tissue with 60% isopropyl alcohol and rinse with pure water. Counterstain the sections with hematoxylin, wash them, and then blue them again. Allow the sections to dry slightly, then add a small amount of glycerol-gelatin and mount them with a coverslip. Avoid introducing air bubbles during this procedure. After preparation, observe the sections under an optical microscope for pathological changes, record them, photograph them, and analyze them.

[0034] Masson staining of liver tissue: After drying, paraffin tissue sections are dewaxed in xylene and then hydrated with a gradient of ethanol to distilled water. Stain the sections with Masson stain. Rinse the sections with distilled water to remove excess stain and differentiate with a weak acid working solution. Dehydrate and clear the sections to obtain complete Masson-stained sections. Once the sections are dry, add a small amount of neutral gum and seal with a coverslip, ensuring that no bubbles are formed. Finally, observe the sections for pathological changes under an optical microscope, documenting, photographing, and analyzing the sections.

[0035] Transmission electron microscopy of liver tissue: The collected liver tissue was fixed in electron microscopy fixative, rinsed thoroughly with PBS, and fixed in 1% osmium phosphate fixative for 2 h. The tissue was then dehydrated, embedded in epoxy resin, and sectioned into ultrathin sections 60-80 nm thick. After staining with uranium-lead, the tissue was observed under a transmission electron microscope and images were acquired.

[0036] 2.7 Data Processing and Analysis All experimental data are expressed as mean ± standard deviation (mean ± SD). One-way analysis of variance (ANOVA) was performed using SPSS 22.0 software, followed by Tukey's test to determine statistical significance. A P value less than 0.05 indicated a significant difference, and a P value less than 0.01 indicated an extremely significant difference.

[0037] 3. Experimental Results 3.1 Effects of white peony tannins on serum liver function and antioxidant indexes in ducklings poisoned by aflatoxin Effects on Serum Liver Function: As shown in Table 1, compared with the control group, the AFB1 group showed significantly decreased ALB, TP, and GLOB levels (P < 0.05), significantly increased TB level (P < 0.05), and significantly increased AST activity, ALP activity, TBA content, and TC content (P < 0.05). Compared with the AFB1 group, the tannin-treated group showed a significant increase in GLOB level (P < 0.05), significantly decreased TB and TBA levels (P < 0.05), and significantly decreased AST and ALP activities (P < 0.05). Compared with the control group, the tannin group showed a significant decrease in GLOB level (P < 0.05), with no significant differences in the other indicators. These results indicate that white peony tannins alone do not cause significant liver damage. However, AFB1 poisoning in ducklings severely impairs liver function, and intervention with white peony tannins can effectively alleviate liver damage.

[0038] Table 1 Effects of AFB1 and white peony tannin on serum liver function in ducklings Note: Different letters in the whole row indicate significant differences (P<0.05), and the same letters or no letters indicate no significant differences (P>0.05).

[0039] Effect of antioxidant indicators: such as Figure 1 As shown in the results, compared with the control group, the serum CAT and SOD activities in the AFB1 group showed a downward trend, while the MDA content was significantly increased (P<0.05). Compared with the AFB1 group, the serum CAT and SOD activities in the tannin treatment group were extremely significantly increased (P<0.01), while the MDA content was extremely significantly decreased (P<0.01). Compared with the control group, the CAT activity in the tannin treatment group was significantly increased (P<0.05), while there were no significant differences in SOD activity and MDA content.

[0040] like Figure 2As shown, compared with the control group, the CAT activity in the liver of the AFB1 group showed a downward trend, the SOD activity and GSH-PX activity were extremely significantly decreased (P<0.01), and the MDA content was extremely significantly increased (P<0.01). Compared with the AFB1 group, the tannin-treated group showed an upward trend in the CAT activity in the liver, the SOD activity was extremely significantly increased (P<0.01), the MDA content was extremely significantly decreased (P<0.01), and the GSH-PX activity was significantly increased (P<0.05). Compared with the control group, the SOD activity in the tannin-treated group was significantly decreased (P<0.05), and there were no significant differences in the CAT activity, GSH-PX activity, and MDA content.

[0041] The above results indicate that tannin extracts can increase the activity of antioxidant enzymes in ducklings, reduce the production of lipid peroxidation product malondialdehyde, and alleviate oxidative damage caused by AFB1 liver damage.

[0042] 3.2 Liver histopathological observation like Figure 3 As shown in the figure, HE staining results showed that compared with the control group, the AFB1 group had swollen hepatocytes, blurred structure, disordered hepatocyte arrangement, unclear hepatic cord structure, and severe hepatocyte vacuolation. Compared with the AFB1 group, the hepatocytes in the tannin treatment group were normal in structure and neatly arranged. Compared with the control group, the liver structure in the tannin treatment group was normal, with no obvious lesions.

[0043] like Figure 4 As shown in the figure, the results of MASSON staining showed that the liver structures of the control group and tannin group were normal, while the liver collagen fiber proliferation was obvious in the AFB1 group; compared with the AFB1 group, the liver fibrosis of the ducklings treated with tannin was improved to the greatest extent.

[0044] like Figure 5 As shown, the results of Oil Red O staining showed that no obvious red lipid droplets were observed in the control group and the tannin group. Compared with the AFB1 group, the red lipid droplets in the tannin treatment group were greatly reduced.

[0045] like Figure 6 As shown in the figure, transmission electron microscopy results showed that the morphological structure of liver cells in the control group and tannin group was clear and complete, while in the AFB1 group, it was obvious that the nucleus was severely deformed, the nuclear membrane and nucleolus were wrinkled and irregular in shape, the mitochondria were deformed, the cristae disappeared, the sizes were uneven and obviously swollen; compared with the AFB1 group, the cell damage in the tannin treatment group was significantly improved, the mitochondria were relatively regular in shape, the cell nucleus was approximately round, the nuclear membrane was relatively complete, and the cytoplasm was uniform.

[0046] Histopathological observations showed that the AFB1 group had significant liver lesions compared to the control group, while treatment with white peony tannins significantly improved liver damage in the ducklings. Histopathological findings in the tannin groups showed no liver damage, confirming the safety of purified tannins at the experimental dose.

[0047] 4. Conclusion The present invention uses Mallard ducks as the research subjects and constructs a subacute aflatoxin poisoning model in ducklings by oral administration of AFB1. The experimental results show that oral administration of AFB1 can significantly reduce the serum liver function indicators ALB, TP, GLOB, and ALP in ducklings, and significantly increase the levels of TBA and AST; liver tissue pathology observation results show that the hepatocytes of ducklings in the AFB1 group are swollen, with blurred structure and disordered arrangement, unclear hepatic cord structure, and severe cell vacuolation. The aflatoxin poisoning model constructed in this study is consistent with the typical manifestations of aflatoxin toxicity models studied by previous researchers, proving the toxic effects of aflatoxin on ducklings and indicating that the aflatoxin poisoning model of this study was successfully constructed.

[0048] The present invention extracts tannin extracts from the traditional Chinese medicine white peony root, and studies the intervention effect of the tannin extracts on AFB1-induced liver damage in ducklings. The experimental results show that, whether from the biochemical and antioxidant indicators of serum and liver tissue or from the pathological changes of liver tissue, a low dose of white peony root tannin extract has a significant therapeutic effect on AFB1-induced liver damage in ducklings.

[0049] It should be noted that the above embodiments are only used to illustrate the present invention, but the present invention is not limited to the above embodiments. Any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention fall within the scope of protection of the present invention.

Claims

1. A Chinese medicinal extract for treating aflatoxin poisoning in ducklings, characterized in that: The Chinese medicine extract is a white peony tannin extract, and the components of the white peony tannin extract include condensed tannins and hydrolyzed tannins. The condensed tannins account for 62% to 63%, and the hydrolyzed tannins account for 37% to 38%.

2. The Chinese medicinal extract for treating aflatoxin poisoning in ducklings according to claim 1, wherein: The condensed tannins include proanthocyanidin trimers and dimers, and the hydrolyzed tannins include 1,2,3,4,6-O-pentagalloylglucose.

3. A Chinese medicinal extract for treating aflatoxin poisoning in ducklings according to claim 1, characterized in that: In the white peony tannin extract, condensed tannins account for 62.83% and hydrolyzed tannins account for 37.17%.

4. Use of the white peony root tannin extract according to claim 1 in treating aflatoxin poisoning in ducklings.

5. A method for preparing a Chinese medicinal extract for treating aflatoxin poisoning in ducklings, characterized in that: The following steps are involved: S1, crush and sieve the white peony root slices, add 50% acetone aqueous solution at a material-liquid ratio of 1:8, and cold soak for 7 days, stirring several times a day; S2, after 7 days, filter and concentrate the filtrate under reduced pressure to recover acetone; S3, add 1% hot gelatin solution to precipitate, let stand, centrifuge, and discard the supernatant; S4, dissolving the precipitate in 50% acetone solution, heating and stirring, cooling to room temperature, centrifuging, and discarding the precipitate; S5, the supernatant was concentrated under reduced pressure and freeze-dried, and the obtained product was purified by Sephadex LH-20, eluted with 50% methanol and 70% acetone in sequence, and the fractions were combined and concentrated to obtain the white peony tannin extract.

6. The preparation method of the Chinese medicinal extract for treating aflatoxin poisoning in ducklings according to claim 5, wherein: In S1, the powder particle size of the white peony root slices after being crushed is 200 mesh.

7. The method for preparing the Chinese medicinal extract for treating aflatoxin poisoning in ducklings according to claim 5, wherein: In S3, the standing time is 15 minutes.

8. The method for preparing the Chinese medicinal extract for treating aflatoxin poisoning in ducklings according to claim 5, wherein: In the above-mentioned S4, the heating temperature is 50°C.

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