Blood cell counting device

Abstract

The invention discloses a blood cell counting device. The blood cell counting device comprises a front tank, a rear tank, a counting hole, at least one detection assembly and a control assembly, wherein the front tank is communicated with the rear tank through the counting hole; the at least one detection assembly is adjacent to the front tank and / or the rear tank; the control assembly is connected with the detection assembly. When blood cell counting is carried out on a to-be-detected sample, the control assembly is used for controlling the detection assembly to detect the liquid level position of the to-be-detected sample and controlling the blood cell counting device to stop blood cell counting when the liquid level position reaches a preset position. Through the blood cell counting device, blood cell counting can be carried out on the to-be-detected sample, and when the liquid level position of the to-be-detected sample in the front pool and / or the rear pool reaches the preset position, it can be determined that the to-be-detected sample with the fixed volume flows through the counting hole, and due to the fact that the to-be-detected sample with the fixed volume contains cells with the fixed particle number, the counting accuracy is improved. The influence on the counting result caused by factors such as the blockage of the counting hole and the size of the counting hole can be avoided, and the counting result is relatively accurate.

Description

Technical Field

[0001] The present application relates to the field of medical technology, in particular to a blood cell counting device. Background Art

[0002] Hematology analyzers are widely used in the field of in vitro diagnostics and have become a standard clinical testing device. The blood cell counting device in a hematology analyzer generally uses the electrical impedance method, which counts blood cells by using pulse recognition to generate electrical pulses as blood cells flow from the front chamber to the counting aperture between the back chamber.

[0003] During operation, due to the small diameter of the counting holes, as well as the adhesion of some proteins and cell debris in the blood around the counting holes, it is difficult to avoid the blockage of the counting holes. Factors such as abnormal blockage of the counting holes and inconsistent counting hole sizes can affect the accuracy of cell counting results. Summary of the Invention

[0004] The present application provides a blood cell counting device that can count blood cells in a sample to be tested. When the liquid level of the sample to be tested in the front tank and / or the rear tank reaches a preset position, it can be determined that a fixed volume of the sample to be tested flows through the counting hole. Since the fixed volume of the sample to be tested contains cells with a fixed number of particles, the counting result can be prevented from being affected by factors such as blockage of the counting hole and the size of the counting hole, thereby making the counting result more accurate.

[0005] To address the aforementioned technical issues, the present application provides, on one hand, a blood cell counting device comprising a forecell, a rear cell, a counting aperture, at least one detection assembly, and a control assembly. The forecell communicates with the rear cell via the counting aperture; at least one detection assembly is disposed adjacent to the forecell and / or the rear cell; and the control assembly is connected to the detection assembly. When counting blood cells in a sample, the control assembly controls the detection assembly to detect the liquid level of the sample and, when the liquid level reaches a predetermined position, controls the blood cell counting device to stop counting blood cells.

[0006] In some embodiments, at least one detection component includes a first detection component, which is arranged adjacent to the front pool and is used to detect whether the liquid level of the sample to be tested drops to a first preset position; and / or at least one detection component includes a second detection component, which is arranged adjacent to the rear pool and is used to detect whether the liquid level of the sample to be tested rises to a second preset position.

[0007] In some embodiments, when counting blood cells of a sample to be tested, the control component is used to control the first detection component to control the blood cell counting device to stop counting blood cells when it detects that the sample to be tested in the front tank has reached a first liquid level position; and / or the control component is used to control the second detection component to control the blood cell counting device to stop counting blood cells when it detects that the sample to be tested in the rear tank has reached a second liquid level position.

[0008] In some embodiments, at least one detection component includes a first detection component and a second detection component, and the first detection component and the second detection component are respectively arranged adjacent to the front pool, and the first detection component and the second detection component are spaced apart along the height direction of the front pool; and / or at least one detection component includes a third detection component and a fourth detection component, and the third detection component and the fourth detection component are respectively arranged adjacent to the rear pool, and the third detection component and the fourth detection component are spaced apart along the height direction of the rear pool.

[0009] In some embodiments, when the control component controls the second detection component to detect that the liquid level of the sample to be tested in the front tank reaches the second liquid level position, the control component controls the blood cell counting device to count the blood cells of the sample to be tested; when the control component controls the first detection component to detect that the sample to be tested in the front tank reaches the first liquid level position, the control component controls the blood cell counting device to stop counting the blood cells.

[0010] In some embodiments, when the control component controls the third detection component to detect that the sample to be tested in the rear pool reaches a third liquid level position, the control component controls the blood cell counting device to count the blood cells of the sample to be tested; when the control component controls the fourth detection component to detect that the sample to be tested in the rear pool reaches a fourth liquid level position, the control component controls the blood cell counting device to stop counting the blood cells.

[0011] In some embodiments, the control component is used to adjust the relative position between the first detection component and the second detection component based on the detection item, and the second detection component is located above the first detection component; or, the control component is used to adjust the relative position between the third detection component and the fourth detection component based on the detection item, and the fourth detection component is located above the third detection component.

[0012] In some embodiments, the blood cell counting device further includes a reagent kit, which is used to set a front pool, a rear pool and a counting hole, the front pool and the rear pool are adjacent to each other, and the rear pool is located on one side of the front pool, and at least one detection component is set outside the reagent kit. When the blood cell counting device completes counting, the reagent kit is replaced.

[0013] In some embodiments, the detection component includes a laser component, and the laser component is used to perform HGB detection on the sample to be tested.

[0014] In some embodiments, the cell counting device further comprises a pipette, which is used to quantitatively transfer the sample to be tested in the reagent kit to the forecell.

[0015] The blood cell counting device provided in some embodiments of the present application includes a front tank, a rear tank, a counting hole, at least one detection component and a control component. Among them, the front tank is connected to the rear tank through the counting hole; at least one detection component is arranged adjacent to the front tank and / or the rear tank; and the control component is connected to the detection component. When counting blood cells of the sample to be tested, the control component is used to control the detection component to detect the liquid level position of the sample to be tested, and control the blood cell counting device to stop counting blood cells when the liquid level position reaches a preset position. By means of the above-mentioned blood cell counting device, blood cells of the sample to be tested can be counted, and when the liquid level position of the sample to be tested in the front tank and / or the rear tank reaches a preset position, it can be determined that a fixed volume of the sample to be tested flows through the counting hole. Since the fixed volume of the sample to be tested contains cells with a fixed number of particles, the influence of factors such as the clogging of the counting hole and the size of the counting hole on the counting result can be avoided, so that the counting result is more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without inventive efforts. Among them:

[0017] Figure 1 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0018] Figure 2 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0019] Figure 3 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0020] Figure 4 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0021] Figure 5 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0022] Figure 6 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0023] Figure 7 is a schematic diagram of a blood cell counting device in some embodiments of the present application;

[0024] Figure 8 is a schematic diagram of the forebay in some embodiments of the present application;

[0025] Figure 9 is a schematic diagram of the forebay in some embodiments of the present application;

[0026] Figure 10 is a schematic diagram of a rear pool in some embodiments of the present application;

[0027] Figure 11 is a schematic diagram of a rear pool in some embodiments of the present application;

[0028] Figure 12 is a schematic cross-sectional view of a blood cell counting device in some embodiments of the present application. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0030] In the related art, a time measurement method is usually used to count blood cells. However, when the hole is clogged or the counting hole size is too small, the counting time used for the blood cell count will be extended even if the volume of the sample to be tested is fixed and the number of particles is fixed. When the counting hole size is too large, the counting time used for the blood cell count will be shortened even if the volume of the sample to be tested is fixed and the number of particles is fixed.

[0031] Based on this, the results obtained by the time measurement method are greatly affected by the equipment / hardware conditions. In order to solve the above technical problems, the present application provides a blood cell counting device.

[0032] See also Figure 1 , Figure 1 Schematic diagram of a blood cell counting device in some embodiments of the present application. The blood cell counting device 10 includes a front pool 101, a rear pool 102, a counting hole 103, at least one detection component 104 and a control component 105.

[0033] Among them, the front pool 101 is connected to the back pool 102 through the counting hole 103, the front pool 101 is connected to the front pool electrode, and the back pool 102 is connected to the back pool electrode; at least one detection component 104 is arranged adjacent to the front pool 101 and / or the back pool 102; the control component 105 is connected to the detection component 104. The front pool 101 and the back pool 102 can be a left-right structure, that is, the back pool 102 is arranged on one side of the front pool 101, such as the left side, right side, upper left corner or other; in other embodiments, the front pool 101 and the back pool 102 can be an up-down structure, such as the back pool 102 is arranged above the front pool 101 or other. It can be understood that Figure 1 The structure shown is only one embodiment of the blood cell counting device 10 of the present application and serves as an illustration. The structure of the blood cell counting device 10 of the present application is not limited by this condition.

[0034] The counting holes 103 include but are not limited to gem holes and micro holes. The diameter of the counting holes 103 can be determined according to actual conditions, such as 60 mm, 70 mm, 80 mm or others.

[0035] In some embodiments, when counting blood cells of a sample to be tested, the control component 105 is used to control the detection component 104 to detect the liquid level of the sample to be tested, and control the blood cell counting device 10 to stop counting blood cells when the liquid level reaches a preset position.

[0036] It can be understood that under the action of the negative pressure provided by the blood cell counting device 10 to the rear tank 102, the sample to be tested in the front tank 101 is transported to the rear tank 102 through the counting hole 103, the liquid level of the sample to be tested in the front tank 101 begins to decrease, and the sample to be tested in the rear tank 102 begins to increase, and the control component 105 controls the blood cell counting device 10 to start blood cell counting; when the liquid level of the sample to be tested in the front tank 101 and / or the rear tank 102 reaches a preset position, the control component 105 controls the blood cell counting device 10 to stop blood cell counting.

[0037] It is worth noting that in some embodiments of the present application, the blood cell counting device 10 stops counting blood cells in the following situations: one is that the impedance detection circuit stops working and no longer generates new counting detection data; the other is that whether the impedance detection circuit stops working has no effect, and the newly generated counting detection data does not participate in the calculation of the final detection result.

[0038] In some embodiments, the number of detection components 104 included in the blood cell counting device 10 can be determined based on factors such as the overall structural volume and cost of the blood cell counting device 10. For example, if the blood cell counting device 10 is required to be a low-cost and small device, the number of detection components 104 in the blood cell counting device 10 can be one.

[0039] In some embodiments, the number of detection components 104 included in the blood cell counting device 10 can be determined according to the test items to be tested on the sample to be tested. For example, if the blood cell counting device 10 is required to perform both PLT (Platelet) and WBC (White Blood Cell) tests, then some detection components 104 can be set to detect the liquid level position of the sample to be tested when performing HGB testing, and some detection components 104 can be set to detect the page position of the sample to be tested when performing WBC testing. When the blood cell counting device 10 can count multiple blood cells (such as WBC, RBC (Red Blood Cell), PLT), the multiple detection components 104 in the blood cell counting device 10 can be differentiated according to the different blood cells to be tested to achieve special use. In this case, the relative positions of the multiple detection components 104 with respect to the front tank 101 and / or the back tank 102 can be set in the same manner, or the positions of the other detection components 104 can be adjusted based on one of the detection components 104 as a reference. For example, the blood cell counting device 10 includes two detection components 104, namely detection component A and detection component B. Detection component A is used when performing PLT detection, and detection component B is used when performing WBC detection. At this time, the position of detection component A can be set first, and then the relative position of detection component B can be determined according to the difference between the volume of the PLT sample and the volume of the WBC sample.

[0040] Furthermore, while the WBC test is being performed on the sample using the detection assembly 104, the HGB (hemoglobin) test can also be performed simultaneously. Specifically, when performing the WBC test, a WBC sample is used. Since a hemolytic agent is added to the sample solution, the hemolytic agent reacts with HGB to lyse red blood cells, forming hemoglobin. This allows for HGB testing, while the lysed red blood cells do not affect the white blood cell count. In other words, the volume of the sample being tested, as well as the volume of the sample passing from the front reservoir 101 through the rear reservoir 102, do not affect the execution of the HGB test.

[0041] In some embodiments, the number of detection components 104 included in the blood cell counting device 10 can be determined according to actual needs. For example, the blood cell counting device 10 includes 1, 2, 3, 4, or other number of detection components 104.

[0042] In some embodiments of the present application, when the blood cell counting device 10 includes one detection component 104, the detection component 104 can be arranged opposite to the front pool 101 / the rear pool 102; when the blood cell counting device 10 includes two detection components 104, the two detection components 104 can be arranged opposite to the front pool 101 and the rear pool 102 respectively, or the two detection components 104 can be arranged opposite to the front pool 101 / the rear pool 102 at the same time; when the blood cell counting device 10 includes other numbers of detection components 104, the detection components 104 can be arranged opposite to the front pool 101 and / or the rear pool 102 according to actual conditions.

[0043] exist Figure 2 In the embodiment, at least one detection component 104 includes a first detection component 1041, which is disposed adjacent to the forebay 101. In this embodiment, the first detection component 1041 is disposed on the right side of the forebay 101 (for illustrative purposes only, and may be located in other positions, which is not limited here). The first detection component 1041 is used to detect whether the liquid level of the sample to be tested has dropped to a first preset position A. When the liquid level drops to the first preset position A, the control component 105 controls the blood cell counting device 10 to stop counting blood cells. The first preset position A can be determined based on the volume of the sample to be tested, the volume of the forebay 101, and / or the test item.

[0044] Specifically, when counting blood cells of the sample to be tested, the control component 105 is used to control the first detection component 1041 to control the blood cell counting device 10 to stop counting blood cells when detecting that the sample to be tested in the forecell 101 reaches the first liquid level position A.

[0045] exist Figure 3 In the embodiment, at least one detection assembly 104 includes a second detection assembly 1042, which is disposed adjacent to the rear reservoir 102. In this embodiment, the second detection assembly 1042 is disposed on the left side of the rear reservoir 102 (this is for illustrative purposes only and may be located in other locations, without limitation). The second detection assembly 1042 is configured to detect whether the liquid level of the sample to be tested has risen to a second preset position B. When the liquid level reaches the second preset position B, the control assembly 105 controls the blood cell counting device 10 to stop counting blood cells. The second preset position B can be determined based on the volume of the sample to be tested, the volume of the rear reservoir 102, and / or the test item.

[0046] Specifically, when counting blood cells of the sample to be tested, the control component 105 is used to control the second detection component 1042 to control the blood cell counting device 10 to stop counting blood cells when detecting that the sample to be tested in the rear tank 102 reaches the second liquid level position B.

[0047] exist Figure 4In the embodiment, at least one detection component 104 includes a first detection component 1041 and a second detection component 1042. The actual arrangement positions of the first detection component 1041 and the second detection component 1042 can be seen in FIG. Figure 2 and Figure 3 , which will not be described in detail here. The first detection component 1041 is used to detect whether the liquid level of the sample to be tested has dropped to a first preset position A, and the second detection component 1042 is used to detect whether the liquid level of the sample to be tested has risen to a second preset position B. When the first detection component 1041 detects that the liquid level has dropped to the first preset position A and the second detection component 1042 detects that the liquid level has risen to the second preset position B, the control component 105 controls the blood cell counting device 10 to stop counting blood cells.

[0048] Specifically, when counting the blood cells of the sample to be tested, the control component 105 is used to control the first detection component 1041 to detect that the sample to be tested in the front tank 101 reaches the first liquid level position A, and to control the second detection component 1042 to detect that the sample to be tested in the rear tank 102 reaches the second liquid level position B, and control the blood cell counting device 10 to stop counting the blood cells.

[0049] In some embodiments, the detection assembly 104 includes a laser assembly. Specifically, the first detection assembly 1041 / second detection assembly 1042 includes a light source transmitter and a photoelectric receiver. The light source transmitter and the photoelectric receiver are disposed opposite the fore pool 101 / rear pool 102, and are disposed on the front and rear sides of the fore pool 101 / rear pool 102, respectively. The light emitted by the light source transmitter can form a light spot on the fore pool 101 / rear pool 102, and the photoelectric receiver can perform photoelectric conversion on the light spot to obtain a voltage signal. The wavelength of the light emitted by the light source transmitter is greater than 800 nm.

[0050] It is worth noting that at the start of counting, the photoelectric receiver reads the voltage signal at a preset period (e.g., 0.1 seconds). When the light spot is covered by the sample to be tested, the photoelectric receiver receives a first voltage signal. When the light spot is level with or below the liquid level of the sample to be tested, due to light refraction, the photoelectric receiver receives a second voltage signal. The second voltage signal differs from the first voltage signal. When the signal difference between the first and second voltage signals exceeds a threshold, the blood cell counting device 10 can be controlled to stop counting. Based on this, the blood cell counting device 10 can be controlled to start / stop blood cell counting based on the voltage signals received by the photoelectric receivers in the first detection component 1041 and / or the second detection component 1042.

[0051] by Figure 2Taking the example shown, the first detection component 1041 is set at the same level as the descent height. At this time, the descent height corresponds to the first preset position A. The light source emitter in the first detection component 1041 forms a light spot at the first preset position A. As the sample to be tested in the fore cell 101 begins to decrease and the blood cell counting device 10 starts to count blood cells, the photoelectric receiver in the first detection component 1041 is opposite to the light source emitter. When the photoelectric receiver detects the light spot at the first preset position A, it indicates that the counting of the sample to be tested is completed, and the control component 105 controls the blood cell counting device 10 to stop counting blood cells.

[0052] by Figure 3 As shown in the example, the second detection component 1042 is set at the same level as the rising height. At this time, the rising height corresponds to the second preset position B. The light source emitter in the second detection component 1042 forms a light spot at the second preset position B. As the sample to be tested in the back pool 102 begins to increase and the blood cell counting device 10 starts to count blood cells, the photoelectric receiver in the second detection component 1042 is opposite to the light source emitter. When the photoelectric receiver detects the light spot at the second preset position B, it means that the counting of the sample to be tested is completed, and the control component 105 controls the blood cell counting device 10 to stop counting blood cells.

[0053] exist Figure 5 In the embodiment, at least one detection component 104 includes a first detection component 1041 and a second detection component 1042, and the first detection component 1041 and the second detection component 1042 are respectively arranged adjacent to the forepool 101, and the first detection component 1041 and the second detection component 1042 are spaced apart along the height direction of the forepool 101; at this time, the first detection component 1041 and the second detection component 1042 are both arranged on the right side of the forepool 101, which is only for illustration; when the control component 105 controls the second detection component 1042 to detect that the liquid level position of the sample to be tested in the forepool 101 reaches the second liquid level position X2, the control component 105 controls the blood cell counting device 10 to count the blood cells of the sample to be tested; when the control component 105 controls the first detection component 1041 to detect that the sample to be tested in the forepool 101 reaches the first liquid level position X1 (which is the same as the liquid level position X2), the control component 105 controls the blood cell counting device 10 to count the blood cells of the sample to be tested; Figure 2 When the first preset position A is shown, the control component 105 controls the blood cell counting device 10 to stop counting blood cells.

[0054] In some embodiments, the first liquid level position X1 is level with the height of the sample to be tested after it descends, and the second liquid level position X2 is level with the initial height of the sample to be tested.

[0055] exist Figure 6In the embodiment, at least one detection component 104 includes a third detection component 1043 and a fourth detection component 1044, and the third detection component 1043 and the fourth detection component 1044 are respectively arranged adjacent to the rear pool 102, and the third detection component 1043 and the fourth detection component 1044 are spaced apart along the height direction of the rear pool 102; at this time, the third detection component 1043 and the fourth detection component 1044 are both arranged on the left side of the rear pool 102, which is only for illustration; when the control component 105 controls the third detection component 1043 to detect that the sample to be tested in the rear pool 102 reaches the third liquid level position X3, the control component 105 controls the blood cell counting device 10 to count the blood cells of the sample to be tested; when the control component 105 controls the fourth detection component 1044 to detect that the sample to be tested in the rear pool 102 reaches the fourth liquid level position X4 (which is the same as the liquid level position X5), the blood cell counting device 10 performs blood cell counting on the sample to be tested; Figure 3 When the second preset position B is reached, the control component 105 controls the blood cell counting device 10 to stop counting blood cells.

[0056] In some embodiments, the third liquid level position X3 is level with the bottom of the rear tank 102 , and the fourth liquid level position X4 is level with the rising height of the sample to be tested in the rear tank 102 .

[0057] exist Figure 7 In the embodiment, at least one detection component 104 includes a first detection component 1041, a second detection component 1042, a third detection component 1043 and a fourth detection component 1044. The actual arrangement positions of the first detection component 1041, the second detection component 1042, the third detection component 1043 and the fourth detection component 1044 can be seen in FIG. Figure 5 and Figure 6 When the control component 105 controls the second detection component 1042 to detect that the liquid level of the sample to be tested in the fore cell 101 has reached the second liquid level position X2, and controls the third detection component 1043 to detect that the sample to be tested in the rear cell 102 has reached the third liquid level position X3, the control component 105 controls the blood cell counting device 10 to count the blood cells of the sample to be tested; when the control component 105 controls the first detection component 1041 to detect that the sample to be tested in the fore cell 101 has reached the first liquid level position X1, and controls the fourth detection component 1044 to detect that the sample to be tested in the rear cell 102 has reached the fourth liquid level position X4, the control component 105 controls the blood cell counting device 10 to stop counting the blood cells.

[0058] In some embodiments of the present application, the control component 105 is used to adjust the relative position between the first detection component 1041 and the second detection component 1042 based on the detection item, and the second detection component 1042 is located above the first detection component 1041; or, the control component 105 is used to adjust the relative position between the third detection component 1043 and the fourth detection component 1044 based on the detection item, and the fourth detection component 1044 is located above the third detection component 1043.

[0059] The blood cell counting device 10 provided in some embodiments of the present application can obtain a highly accurate blood cell count by using the first detection component 1041 and / or the second detection component 1042 in combination, or using the first detection component 1041 and the second detection component 1042 in combination, or using the third detection component 1043 and the fourth detection component 1044 in combination, or using the first detection component 1041 and the second detection component 1042 in combination, and the third detection component 1043 and the fourth detection component 1044 in combination.

[0060] In other embodiments, at least one detection component 104 includes a first detection component 1041, a third detection component 1043, and a fourth detection component 1044. Figure 2 As shown in the figure, the third detection component 1043 and the fourth detection component 1044 are arranged as shown in the figure. Figure 6 The settings shown are not described here in detail.

[0061] In other embodiments, at least one detection component 104 includes a second detection component 1042, a third detection component 1043, and a fourth detection component 1044. Figure 3 As shown in the figure, the third detection component 1043 and the fourth detection component 1044 are arranged as shown in the figure. Figure 6 In some embodiments of the present application, the actual placement of the detection components 104 in the blood cell counting device 10 may vary depending on the number of detection components 104 .

[0062] In some embodiments of the present application, the actual location of the detection component 104 in the blood cell counting device 10 may vary depending on the volume of the sample to be tested.

[0063] The blood cell counting device 10 provided in some embodiments of the present application can perform blood cell counting on a sample. Because a fixed volume of sample contains a fixed number of cells, the counting result can be accurately determined by factors such as clogging of the counting apertures 103 and differences in the size of the counting apertures 103 between different devices. Furthermore, the number and actual placement of the detection components 104 can be determined based on whether the total volume of the sample within the forebay 101 is known, providing greater flexibility.

[0064] In some embodiments of the present application, Figures 1 to 7 As shown, the front pool 101 and the rear pool 102 are adjacently arranged, and the rear pool 102 is located on one side of the front pool 101, and the counting hole 103 is arranged near the bottom of the front pool 101. It is understood that the front pool 101 can be arranged on the left side, right side, bottom side or other positions of the rear pool 102, which is not limited here.

[0065] In some embodiments of the present application, the front pool 101 and the rear pool 102 are arranged adjacent to each other, and the flow direction of the sample to be tested is left and right. At this time, the detection direction between the light source emitter and the photoelectric receiver in the detection component 104 can be front and back. These two directions are perpendicular to each other and do not interfere with each other.

[0066] In some embodiments, as Figure 8 As shown, the front pool 101 includes an integrally formed first part 1011 and a second part 1012, the cross-sectional area of ​​the first part 1011 is larger than the cross-sectional area of ​​the second part 1012, the light transmittance of the second part 1012 is greater than the light transmittance of the first part 1011, and the first detection component 1041 and the second detection component 1042 are arranged adjacent to the second part 1012.

[0067] In some embodiments, as Figure 9 As shown, the forecell 101 includes a forecell shell 201 and a forecell electrode 202. The forecell shell 201 includes an integrally formed first portion 1011 and a second portion 1012. A through hole is provided at the bottom of the forecell shell 201, and one end of the forecell electrode 202 contacts the sample to be tested loaded in the forecell shell 201 through the through hole.

[0068] In some embodiments, as Figure 10 As shown, the rear pool 102 includes an integrally formed first part 1021 and a second part 1022, the cross-sectional area of ​​the first part 1021 is smaller than the cross-sectional area of ​​the second part 1022, the light transmittance of the second part 1022 is smaller than the light transmittance of the first part 1021, and the third detection component 1043 and the fourth detection component 1044 are arranged adjacent to the first part 1021.

[0069] In some embodiments, as Figure 11As shown, the back cell 102 includes a back cell shell 301 and a back cell electrode 302. The back cell shell 301 includes an integrally formed first portion 1021 and a second portion 1022. One end of the back cell electrode 302 is disposed in the cavity of the back cell shell 301, and the other end of the back cell electrode 302 is disposed outside the cavity of the back cell shell 301.

[0070] In some embodiments, the detection component 104 in the blood cell counting device 10 is as follows: Figure 6 When the third detection component 1043 and the fourth detection component 1044 are included, the third detection component 1043 can be arranged above the rear cell electrode 302 to avoid interference with the voltage signal between the front cell electrode 202 and the rear cell electrode 302, and to avoid the front cell electrode 202 and the rear cell electrode 302 from affecting the formation of the third detection component 1043.

[0071] join Figure 12 , Figure 12 Figure 1 is a schematic cross-sectional view of a blood cell counting device 10 according to some embodiments of the present application. The blood cell counting device 10 includes a front cell 101, a rear cell 102, a counting aperture 103, at least one detection assembly 104 (for illustrative purposes, only the position of the light spot generated by the light source emitter in the first detection assembly 1041 is shown), a control assembly 105 (not shown), a reagent cartridge 106, an electrode insertion seat 107, and a negative pressure chamber 108.

[0072] The reagent cartridge 106 is provided with a front cell 101, a rear cell 102, and a counting well 103. The front cell 101 and rear cell 102 are positioned adjacent to each other, with the rear cell 102 positioned to one side of the front cell 101. At least one detection component 104 is positioned external to the reagent cartridge 106. When the blood cell counting device 10 completes counting, the reagent cartridge 106 is replaced. The detection component 105 is not discarded with the reagent cartridge 106 and is reusable. It does not come into contact with the liquid within the reagent cartridge 106 and does not require cleaning.

[0073] Among them, the front pool 101 includes a front pool shell 201 and a front pool electrode 202; the back pool 102 includes a back pool shell 301 and a back pool electrode 302; the electrode penetration seat 107 is connected to the front pool shell 201 through the through hole at the bottom of the front pool shell 201, one end of the front pool electrode 202 contacts the sample to be tested in the front pool shell 201 through the through hole, and the other end of the front pool electrode 202 is arranged outside the cavity of the electrode penetration seat 107; the negative pressure chamber 108 is connected to the front pool 101, and the negative pressure chamber 108 is used to apply negative pressure to the sample to be tested in the front pool 101, so that the sample to be tested is in the negative pressure. Under the action of pressure, the cells pass through the counting hole 103 one by one. Since the two ends of the counting hole 103, i.e. the front pool 101 and the back pool 102, are respectively provided with the front pool electrode 202 and the back pool electrode 302 that can provide current, when the cells in the sample to be tested pass through the counting hole 103, the voltage at both ends of the counting hole 103 will change, forming pulses related to the cell morphology. The number of pulses is the number of cells, and the size of the pulse amplitude can represent the size of the cell amplitude. Based on this, the number of blood cells (such as HGB, WBC, RBC, PLT) can be calculated.

[0074] in, Figure 12 The light spot position shown can be the first liquid level position X1 or the third liquid level position X3, which is determined according to actual conditions.

[0075] In some embodiments, as Figure 12 As shown, the blood cell counting device 10 further includes a plurality of pools, which are arranged on the right side of the front pool 101. The pool and the rear pool 102 are respectively arranged on both sides of the front pool 101. The pools are used to transport samples to be tested, such as HGB samples, WBC samples, and PLT samples, to the front pool 101, or to transport cleaning fluid to the front pool 101 to clean the front pool 101 and the rear pool 102.

[0076] In some embodiments, the blood cell counting device 10 further includes a pipette (not shown) for quantitatively transferring the sample to be tested from the reagent cartridge 106 to the forecell 101. When there is only one detection assembly 104, a high-precision pipette can be used to quantify the sample to be tested to ensure the total volume of the sample to be tested before the start of the test. During the test process, when the sample to be tested passes through the counting aperture 103 and causes the light spot signal of the detection assembly 104 to change, the volume passing through the counting aperture 103 is the fixed volume required for counting, and the error is negligible.

[0077] In summary, the blood cell counting device 10 provided in the present application can count the sample to be tested, and the blood cell counting is performed by extracting a fixed volume of the sample to be tested, and the measurement result is accurate.

[0078] The above description is only an implementation method of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the description and drawings of this application, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A blood cell counting device, characterized in that: The blood cell counting device comprises: A front pool, a rear pool and a counting hole, wherein the front pool is connected to the rear pool through the counting hole; at least one detection assembly disposed adjacent to the front tank and / or the rear tank; a control component connected to the detection component; When counting blood cells of the sample to be tested, the control component is used to control the detection component to detect the liquid level position of the sample to be tested, and control the blood cell counting device to stop counting blood cells when the liquid level position reaches a preset position.

2. The blood cell counting device according to claim 1, wherein The at least one detection component includes a first detection component, the first detection component is arranged adjacent to the forebay, and the first detection component is used to detect whether the liquid level of the sample to be tested drops to a first preset position; And / or the at least one detection component includes a second detection component, the second detection component is arranged adjacent to the back pool, and the second detection component is used to detect whether the liquid level of the sample to be tested rises to a second preset position.

3. The blood cell counting device according to claim 2, characterized in that When counting the blood cells of the sample to be tested, the control component is used to control the blood cell counting device to stop counting the blood cells when the first detection component detects that the sample to be tested in the front tank reaches a first liquid level position; and / or the control component is used to control the blood cell counting device to stop counting the blood cells when the second detection component detects that the sample to be tested in the rear tank reaches a second liquid level position.

4. The blood cell counting device according to claim 1, wherein The at least one detection component includes a first detection component and a second detection component, the first detection component and the second detection component are respectively arranged adjacent to the front pool, and the first detection component and the second detection component are spaced apart along the height direction of the front pool; and / or the at least one detection component includes a third detection component and a fourth detection component, the third detection component and the fourth detection component are respectively arranged adjacent to the rear pool, and the third detection component and the fourth detection component are spaced apart along the height direction of the rear pool.

5. The blood cell counting device according to claim 4, characterized in that: When the control component controls the second detection component to detect that the liquid level of the sample to be tested in the fore cell reaches the second liquid level, the control component controls the blood cell counting device to count the blood cells of the sample to be tested; When the control component controls the first detection component to detect that the sample to be tested in the fore cell reaches a first liquid level position, the control component controls the blood cell counting device to stop blood cell counting.

6. The blood cell counting device according to claim 4, characterized in that When the control component controls the third detection component to detect that the sample to be tested in the rear tank reaches a third liquid level position, the control component controls the blood cell counting device to count the blood cells of the sample to be tested; When the control component controls the fourth detection component to detect that the sample to be tested in the rear pool reaches a fourth liquid level position, the control component controls the blood cell counting device to stop blood cell counting.

7. The blood cell counting device according to claim 4, characterized in that The control component is used to adjust the relative position between the first detection component and the second detection component based on the detection item, and the second detection component is located above the first detection component; or, the control component is used to adjust the relative position between the third detection component and the fourth detection component based on the detection item, and the fourth detection component is located above the third detection component.

8. The blood cell counting device according to claim 1, wherein: The blood cell counting device also includes a reagent kit, which is used to set the front pool, the rear pool and the counting hole. The front pool and the rear pool are adjacent to each other, and the rear pool is located on one side of the front pool. The at least one detection component is set outside the reagent kit. When the blood cell counting device completes counting, the reagent kit is replaced.

9. The blood cell counting device according to claim 1, wherein: The detection component includes a laser component, and the laser component is used to perform HGB detection on the sample to be tested.

10. The blood cell counting device according to claim 8, characterized in that: The cell counting device further comprises a pipette, which is used to quantitatively transfer the sample to be tested in the reagent kit to the fore cell.

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