Immunity-enhancing and anti-aging tea oil health-care capsule and preparation method thereof

Abstract

The invention discloses an immunity-enhancing and anti-aging tea oil health-care capsule and a preparation method thereof, and belongs to the technical field of food processing. The capsule comprises a capsule shell and a capsule content, and the capsule content comprises the following components in parts by weight: 20-40 parts of camellia oil, 12-20 parts of propolis, 8-10 parts of lucid ganoderma, 8-10 parts of American ginseng, 7-9 parts of Chinese wolfberry, 7-9 parts of Chinese yam, 5-7 parts of pyrroloquinoline quinone, 5-7 parts of yeast, 5-7 parts of vitamin E, 4-6 parts of collagen peptide and 4-6 parts of a grape seed extract. According to the capsule, through the design of the formula of the contents, all the components cooperate with one another, the good immunity enhancing and anti-aging effects are achieved, the bioavailability of key components is improved, and the production cost is reduced.

Description

Technical Field

[0001] The present application relates to a tea oil health-care capsule capable of enhancing immunity and resisting aging and a preparation method thereof, and belongs to the field of food processing technology. Background Art

[0002] With the improvement of healthcare systems and rising economic levels, the average life expectancy of the global population is increasing, and population aging is becoming a universal trend that countries around the world are currently experiencing or will soon experience. Therefore, the prevention and treatment of aging-related diseases are becoming a growing focus of attention. Aging refers to the phenomenon of aging-related degeneration of tissue and organ structure and physiological function, as well as a decline in the ability to resist metabolic stress, which occurs with aging after the body reaches maturity. According to existing scientific research, aging is characterized by 12 major hallmarks, including altered intercellular communication, stem cell exhaustion, dysbiosis, chronic inflammation, epigenetic changes, loss of genomic stability, imbalanced proteostasis, telomere attrition, macroautophagy impairment, mitochondrial dysfunction, dysregulated nutrient sensing, and cellular senescence. Telomere shortening is one of the leading biomarkers of cellular aging, and in recent years, regulating telomere length and telomerase activity has emerged as a new anti-aging strategy.

[0003] At the same time, aging can lead to immune system dysfunction, which not only reduces resistance to infection but also triggers low-intensity inflammation, which in turn leads to mitochondrial dysfunction and the accumulation of senescent cells, accelerating the aging process. Therefore, achieving the dual effects of anti-aging and enhancing human immunity is a pressing technical challenge in preventing and treating aging-related diseases and extending healthy lifespan. Summary of the Invention

[0004] In order to solve the above problems, a tea oil health capsule for enhancing immunity and anti-aging and a preparation method thereof are provided. Through the synergistic effect of the composition, the body's immunity is enhanced while effectively regulating the aging of mitochondria, thereby achieving an anti-aging effect.

[0005] According to one aspect of the present application, a tea oil health capsule for enhancing immunity and anti-aging is provided, characterized in that it comprises a capsule skin and capsule contents, and the capsule contents include the following components in parts by weight: 20-40 parts of camellia oil, 12-20 parts of propolis, 8-10 parts of Ganoderma lucidum, 8-10 parts of American ginseng, 7-9 parts of wolfberry, 7-9 parts of yam, 5-7 parts of pyrroloquinoline quinone, 5-7 parts of yeast, 5-7 parts of vitamin E, 4-6 parts of collagen peptide, and 4-6 parts of grape seed extract.

[0006] Camellia oil contains high levels of monounsaturated fatty acids, camellia glycosides, tea polyphenols, saponins, tannins, and squalene. Tea polyphenols and tannins can scavenge superoxide free radicals in the body, reducing oxidative stress damage to immune cells and maintaining their activity. Saponins help regulate the body's inflammatory response, reducing the weakening of immunity caused by chronic inflammation. Rich in unsaturated fatty acids, a crucial component of cell membranes, they help maintain the normal function of immune cells. Squalene binds to free radicals, acting as an antioxidant and regulating the activity of antioxidant enzymes. Propolis is rich in flavonoids, phenolic acids, terpenes, and other bioactive substances, which not only resist pathogen invasion but also stimulate the activity of immune cells such as macrophages, T lymphocytes, and B lymphocytes, enhancing the immune response. In Traditional Chinese Medicine, Lingzhi (Ganoderma lucidum) boasts the ability to replenish qi and calm the mind, treating restlessness, insomnia, palpitations, and asthenia. Its polysaccharides and triterpenoids promote immune cell differentiation and exert bidirectional regulatory effects, inhibiting excessive immune responses and preventing allergies and autoimmune diseases associated with enhanced immunity. They also promote DNA synthesis. American ginseng enhances immune cell activity while also improving the body's ability to adapt to stress, alleviating fatigue and mitigating the negative effects of stress on the immune system. Lycium barbarum and Chinese yam, rich in polysaccharides, cellulose, minerals, and soluble fiber, work synergistically with yeast to regulate intestinal flora, strengthen the spleen and stomach, and support the intestine's role as an immune organ. They also stimulate essential peptides such as glutathione, as well as fibroblast proliferation and collagen synthesis, protecting mitochondrial function and delaying cell aging. Furthermore, the beta-glucan in yeast activates immune cell activity, thereby stimulating the immune system and enhancing immunity.

[0007] The overall formula can work synergistically to achieve multi-target activation and effectively enhance immune activity, while providing an antibacterial and antiviral immune environment, making the activation of acquired immunity safer and more reliable; it can provide a powerful antioxidant and anti-inflammatory network, effectively scavenge free radicals, reduce the immune burden caused by inflammation, and reduce oxidative damage; it provides rich nutrition and energy support, and provides the necessary nutrients and energy basis for the normal metabolism, value-added and function of cells.

[0008] Vitamin E and polyphenols such as proanthocyanidins, catechins, epicatechin, gallic acid, epicatechin, and gallic acid esters in grape seed extract form a powerful antioxidant network, improving the overall antioxidant capacity. Vitamin E and squalene work synergistically to jointly inhibit peroxidation reactions in a lipid environment and protect immune cells from oxidative damage.

[0009] Pyrroloquinoline quinone, an anti-aging molecule difficult for the human body to synthesize, activates longevity proteins and promotes mitochondrial regeneration. It also scavenges reactive oxygen species (ROS) generated by hypoxia-reperfusion and significantly reduces the release of lactate dehydrogenase in the heart. Its catalytic product, catalyzed by flavin reductase, also reduces hemoglobin peroxidation, eliminating myocardial damage caused by hypoxia-reperfusion. Combining these ingredients with the aforementioned ingredients can improve the absorption and bioavailability of pyrroloquinoline quinone and reduce the amount of pyrroloquinoline quinone raw material, thereby maintaining its anti-aging effects while reducing production costs.

[0010] Specifically, the preparation method of the grape seed extract includes:

[0011] P1. Wash and drain the grape seeds, then crush them to obtain 40-mesh grape seed powder.

[0012] P2. The grape seed powder was fully dispersed in water, the pH was adjusted to 5-5.5, 2-3% pectinase by dry weight of grape seed powder was added and the pectin was treated at 40-60 ° C for 6-10h;

[0013] P3. Lactobacillus plantarum and Bacillus subtilis were cultured in a mass ratio of 1:(1-3) in MRS medium supplemented with 2-3% (m / V) grape seed powder at 30-40°C and 100 rpm for 25-30 hours to serve as inoculum;

[0014] P4. After the grape seed powder was sterilized by pectin treatment, 15-20wt% of the seed bacteria was added to the grape seed powder and fermented in MRS medium at 30-40°C and 150rpm for 35-40 hours to obtain a grape seed fermentation product;

[0015] P5. The grape seed fermentation product was added to a 70-75% ethanol aqueous solution at a ratio of 1:20 (m / V), ultrasonically extracted for 30 minutes, and the supernatant was centrifuged;

[0016] P6. The grape seed extract aqueous solution was adsorbed on LX-1600 resin and desorbed with 65-75% ethanol to obtain a refined grape seed extract solution;

[0017] P7. The obtained refined grape seed extract solution was concentrated under reduced pressure at 50-60°C at 0.02-0.08 MPa and then spray-dried with an inlet temperature of 185°C and an outlet temperature of 85°C.

[0018] Preferably, the mass ratio of Ganoderma lucidum to American ginseng, wolfberry and Chinese yam is 1:1:(0.75-0.8):(0.75-0.8).

[0019] Preferably, the preparation is made from the following components by weight: 30 parts of camellia oil, 16 parts of propolis, 9 parts of Ganoderma lucidum, 9 parts of American ginseng, 8 parts of wolfberry, 8 parts of yam, 6 parts of pyrroloquinoline quinone, 6 parts of yeast, 6 parts of vitamin E, 5 parts of collagen peptide, and 5 parts of grape seed extract.

[0020] Preferably, the capsule skin comprises the following components in parts by weight: 50-65 parts of sago starch, 33-35 parts of pectin, 20-23 parts of carrageenan, 5-10 parts of xanthan gum, 20-25 parts of glycerol, 1-3 parts of β-glucan, 1-3 parts of citric acid, and 10-20 parts of purified water.

[0021] In this solution, the colloidal materials used are sago starch, pectin, carrageenan, and xanthan gum, which replace traditional gelatin, meet the plant-based requirements, and provide an elastic taste; the plasticizer glycerin can increase flexibility and moisturizing properties, and β-glucan improves the stability of the gel structure and the water retention of the gel, solving the defect of plant gum being easy to dry and harden, extending the shelf life, and at the same time having an immune-regulating effect; citric acid adjusts the pH of the gel to neutral to enhance stability.

[0022] According to another aspect of the present application, a method for preparing an immunity-enhancing and anti-aging tea oil health capsule is provided, which is characterized by comprising the following steps:

[0023] S1. Prepare camellia oil, yeast powder, Chinese medicine powder, propolis juice, capsule skin;

[0024] S2. adding pyrroloquinoline quinone, dry yeast powder, vitamin E, collagen peptide, and grape seed extract to camellia oil to obtain a mixed camellia oil;

[0025] S3. The herbal powder was thoroughly mixed with water and heated for 40-50min, then filtered, and propolis was added while hot, and mixed thoroughly to obtain a premix;

[0026] S4 premixed liquid after cooling, add S3 obtained mixed camellia oil, mix thoroughly, that is, the contents of the capsule;

[0027] S5. Filling the capsule contents into the capsule skin to prepare tea oil capsules.

[0028] Specifically, the propolis juice is the propolis juice obtained by melting and filtering Italian bee propolis.

[0029] Preferably, the camellia oil is obtained by cold pressing the camellia seeds and filtering them, and the cold pressing temperature is 70-80°C.

[0030] Preferably, the Chinese medicinal powder is obtained by mechanically crushing ganoderma lucidum, American ginseng, wolfberry and yam, and passing through a 650-750 mesh sieve.

[0031] Preferably, in step S3, the mass ratio of the Chinese medicinal powder to water is 1:(0.3-0.5), the heating pressure is 140-160 kPa, and the heating temperature is 105-121°C.

[0032] Preferably, the premixed solution in step S4 needs to be cooled to 20-30°C.

[0033] Preferably, the preparation method of the capsule skin comprises: pre-swelling sago starch, pectin, carrageenan and xanthan gum in purified water, then heating and stirring to dissolve, adding glycerol, β-glucan and citric acid, homogenizing and cooling, adjusting the pH to 3.8, and setting aside.

[0034] The pre-swelling conditions are swelling at 40-50°C for 30-40 minutes; secondary heating to 60-70°C, stirring speed 200-500rpm; homogenization speed 4000-5000rpm, homogenization for 10-15 minutes and then cooling to 40°C.

[0035] The beneficial effects of this application include but are not limited to:

[0036] 1. According to the tea oil health capsule for enhancing immunity and anti-aging of the present application, the formula design of the capsule contents enables synergistic enhancement between the components, achieves multi-target activation, and effectively improves immune activity, while providing an antibacterial and antiviral immune environment, making the activation of acquired immunity safer and more reliable; it can provide a powerful antioxidant and anti-inflammatory network, effectively scavenge free radicals, reduce the immune burden caused by inflammation, and reduce oxidative damage; it provides rich nutrition and energy support, and provides the necessary nutrients and energy basis for the normal metabolism, value-added and function of cells.

[0037] 2. According to the tea oil health capsules for enhancing immunity and anti-aging of the present application, in terms of the traditional Chinese medicine formula, American ginseng and Ganoderma lucidum are used as the main medicines to enhance the immune system from two aspects of bidirectional balanced regulation of the immune system and relief of body stress to prevent over-stimulation of the immune system. Propolis is used as the assistant medicine to provide an antibacterial and antiviral environment. Wolfberry and yam are used as adjuvant medicines to provide rich nutrients to ensure the synthesis of immune cells and cell aging. Together with other components, the formula plays the role of enhancing immunity and anti-aging.

[0038] 3. According to the immunity-enhancing and anti-aging camellia oil health capsules of the present application, the capsule skin is based on sago starch, which has high transparency and high gel strength, and is green, environmentally friendly and pollution-free; at the same time, the addition of phospholipids enhances stability and greatly optimizes the emulsification properties of the oil in the capsule, making it easier to absorb; the prepared camellia oil capsules have beautiful appearance, good taste, and are easy to carry. They can be widely used for different groups of people, making up for the shortcomings of existing products on the market in terms of single target population, and have high market potential. DETAILED DESCRIPTION

[0039] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0040] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. The reagents and raw materials used in the present invention can be purchased through conventional channels. Unless otherwise specified, the reagents and raw materials used in the present invention are used in accordance with conventional methods in the art or in accordance with the product instructions. In addition, any methods and materials similar to or equivalent to those described herein can be applied to the present invention. The preferred embodiments and materials described in this patent are for illustrative purposes only.

[0041] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0042] The raw materials used in this plan can be purchased through the following channels:

[0043] Vitamin E: Shandong Pingju Biotechnology Co., Ltd.;

[0044] Yeast: Angel Yeast Co., Ltd.;

[0045] Collagen peptide: Shenzhen Jihechang New Materials Co., Ltd.;

[0046] The grape seed extract used in Examples 1-3 was purchased from Shandong Yatu Biotechnology Co., Ltd.

[0047] Grape seed: Chengdu Pusi Biotechnology Co., Ltd.

[0048] Basic information of the raw materials in this solution:

[0049] American ginseng: sweet, slightly bitter, cold in nature; replenishes Qi, benefits lung yin, clears internal heat, promotes fluid production and quenches thirst;

[0050] Lingzhi: sweet, neutral, non-toxic; replenishes Qi and blood, soothes the mind, and strengthens the spleen and stomach;

[0051] Wolfberry: sweet in taste, neutral in nature; nourishes the kidneys, moistens the lungs, replenishes the liver, and improves eyesight;

[0052] Chinese Yam: sweet, neutral; nourishes the spleen and stomach, promotes fluid production and benefits the lungs, nourishes the kidneys and astringes essence;

[0053] Propolis: bitter, pungent, and cold; replenishes weakness, transforms turbid fat, and relieves thirst.

[0054] The intermediate substances used in this scheme are prepared by the following method:

[0055] Chinese herbal medicine powder: Ganoderma lucidum, American ginseng, wolfberry and yam are mechanically crushed and passed through a 650-750 mesh sieve to obtain Chinese herbal medicine powder.

[0056] Propolis juice: Propolis juice obtained by melting and filtering Italian bee propolis.

[0057] Camellia oil: Camellia seeds are cold-pressed at 75°C and filtered to obtain camellia oil.

[0058] Capsule skin: Swell sago starch, pectin, carrageenan, and xanthan gum in purified water at 40-50°C for 30-40 minutes, then heat to 60°C and stir to dissolve at 400 rpm. Add glycerol, β-glucan, and citric acid, homogenize at 4500 rpm for 15 minutes, and cool to 40°C. Adjust pH to 3.8 and set aside.

[0059] The components of the capsule skin are: 60 parts of sago starch, 34 parts of pectin, 22 parts of carrageenan, 8 parts of xanthan gum, 22 parts of glycerol, 2 parts of beta-glucan, 2 parts of citric acid, and 15 parts of purified water.

[0060] Example 1

[0061] Preparation of tea oil health capsules for enhancing immunity and resisting aging:

[0062] S1. Prepare camellia oil, yeast powder, Chinese medicine powder, propolis juice, capsule skin;

[0063] S2. adding pyrroloquinoline quinone, dry yeast powder, vitamin E, collagen peptide, and grape seed extract to camellia oil to obtain a mixed camellia oil;

[0064] S3. Add 0.3 times the mass ratio of water to the herbal powder and mix thoroughly and heat to 105 ° C at 140kPa, filter after heating for 40min, cool to 65 ° C, add propolis while hot, mix thoroughly to obtain a premix;

[0065] S4. After the premixed liquid was cooled to 20°C, the mixed camellia oil obtained in S3 was added and mixed thoroughly to obtain the contents of the capsule;

[0066] S5. Filling the capsule contents into the capsule skin to prepare tea oil capsules.

[0067] The components of the capsule contents are: 20 parts of camellia oil, 12 parts of propolis, 8 parts of ganoderma lucidum, 8 parts of American ginseng, 7 parts of wolfberry, 7 parts of yam, 5 parts of pyrroloquinoline quinone, 5 parts of yeast, 5 parts of vitamin E, 4 parts of collagen peptide, and 4 parts of grape seed extract.

[0068] Example 2

[0069] Preparation of tea oil health capsules for enhancing immunity and resisting aging:

[0070] S1. Prepare camellia oil, yeast powder, Chinese medicine powder, propolis juice, capsule skin;

[0071] S2. adding pyrroloquinoline quinone, dry yeast powder, vitamin E, collagen peptide, and grape seed extract to camellia oil to obtain a mixed camellia oil;

[0072] S3. Add 0.5 times the mass ratio of Chinese herbal powder to water and mix thoroughly and heat to 121 ° C at 160kPa, filter after heating for 50min, cool to 65 ° C, add propolis while hot, mix thoroughly to obtain a premix;

[0073] S4. After the premixed liquid was cooled to 30°C, the mixed camellia oil obtained in S3 was added and mixed thoroughly to obtain the contents of the capsule;

[0074] S5. Filling the capsule contents into the capsule skin to prepare tea oil capsules.

[0075] The components of the capsule contents are: 40 parts of camellia oil, 20 parts of propolis, 10 parts of Ganoderma lucidum, 10 parts of American ginseng, 9 parts of wolfberry, 9 parts of yam, 7 parts of pyrroloquinoline quinone, 7 parts of yeast, 7 parts of vitamin E, 6 parts of collagen peptide, and 6 parts of grape seed extract.

[0076] Example 3

[0077] Preparation of tea oil health capsules for enhancing immunity and resisting aging:

[0078] S1. Prepare camellia oil, yeast powder, Chinese medicine powder, propolis juice, capsule skin;

[0079] S2. adding pyrroloquinoline quinone, dry yeast powder, vitamin E, collagen peptide, and grape seed extract to camellia oil to obtain a mixed camellia oil;

[0080] S3. Add 0.4 times the mass ratio of water to the herbal powder and mix thoroughly and heat to 115 ° C at 150kPa, filter after heating for 45min, cool to 65 ° C, add propolis while hot, mix thoroughly to obtain a premix;

[0081] S4. After the premixed liquid was cooled to 25°C, the mixed camellia oil obtained in S3 was added and mixed thoroughly to obtain the contents of the capsule;

[0082] S5. Filling the capsule contents into the capsule skin to prepare tea oil capsules.

[0083] The components of the capsule contents are: 30 parts of camellia oil, 16 parts of propolis, 9 parts of ganoderma lucidum, 9 parts of American ginseng, 8 parts of wolfberry, 8 parts of yam, 6 parts of pyrroloquinoline quinone, 6 parts of yeast, 6 parts of vitamin E, 5 parts of collagen peptide, and 5 parts of grape seed extract.

[0084] Example 4

[0085] The difference from Example 3 is that the preparation method of grape seed extract in the components of the capsule content is as follows:

[0086] P1. Wash and drain the grape seeds, then crush them to obtain 40-mesh grape seed powder.

[0087] P2. The grape seed powder was fully dispersed in water, the pH was adjusted to 5, and 2% pectinase by dry weight of grape seed powder was added and the pectin was treated at 40°C for 10h;

[0088] P3. Lactobacillus plantarum and Bacillus subtilis were cultured in a mass ratio of 1:3 in MRS medium supplemented with 2% (m / v) grape seed powder at 30°C and 100 rpm for 30 hours to serve as inoculum.

[0089] P4. After the grape seed powder was sterilized by pectin treatment, 15 wt% of the seed was added to the grape seed powder and fermented in MRS medium at 40 ° C and 150 rpm for 40 hours to obtain a grape seed fermentation product;

[0090] P5. The grape seed fermentation product was added to a 70% ethanol-water solution at a ratio of 1:20 (m / v). Ultrasonic extraction was performed for 30 min, and the supernatant was collected by centrifugation.

[0091] P6. The grape seed extract aqueous solution was adsorbed on LX-1600 resin and desorbed with 65% ethanol to obtain a refined grape seed extract solution;

[0092] P7. The obtained refined grape seed extract solution was concentrated under reduced pressure at 50°C at 0.02 MPa and then spray-dried with an inlet temperature of 185°C and an outlet temperature of 85°C.

[0093] Example 5

[0094] The difference from Example 3 is that the preparation method of grape seed extract in the components of the capsule content is as follows:

[0095] P1. Wash and drain the grape seeds, then crush them to obtain 40-mesh grape seed powder.

[0096] P2. The grape seed powder was fully dispersed in water, the pH was adjusted to 5.5, and 3% pectinase by dry weight of grape seed powder was added and the pectin was treated at 40-60 ° C for 6h;

[0097] P3. Lactobacillus plantarum and Bacillus subtilis were cultured in a 1:1 mass ratio in MRS medium supplemented with 3% (m / v) grape seed powder at 30°C and 100 rpm for 30 hours to serve as inoculum.

[0098] P4. After the grape seed powder was sterilized by pectin treatment, 20 wt% of the seed was added to the grape seed powder and fermented in MRS medium at 30 ° C and 150 rpm for 35 hours to obtain a grape seed fermentation product;

[0099] P5. The grape seed fermentation product was added to a 75% ethanol-water solution at a ratio of 1:20 (m / v). Ultrasonic extraction was performed for 30 min, and the supernatant was collected by centrifugation.

[0100] P6. The grape seed extract aqueous solution was adsorbed on LX-1600 resin and desorbed with 75% ethanol to obtain a refined grape seed extract solution;

[0101] P7. The obtained refined grape seed extract solution was concentrated under reduced pressure at 0.08 MPa and 60°C and then spray-dried with an inlet temperature of 185°C and an outlet temperature of 85°C.

[0102] Example 6

[0103] The difference from Example 4 is that the fermentation bacteria used are Saccharomyces cerevisiae and Bacillus subtilis in a mass ratio of 1:1.

[0104] Example 7

[0105] The difference from Example 4 is that the grape seed powder is first fermented to obtain a grape seed fermentate, which is then subjected to enzymatic hydrolysis, and then subjected to subsequent treatment to obtain the grape seed extract.

[0106] Example 8

[0107] The difference from Example 3 is that the composition of the Chinese medicine powder is 10 parts of Ganoderma lucidum, 10 parts of American ginseng, 7 parts of wolfberry, and 7 parts of Chinese yam.

[0108] Comparative Example 1

[0109] The difference from Example 3 is that the number of pyrroloquinoline quinone in the components of the capsule content is 20 parts.

[0110] Comparative Example 2

[0111] The difference from Example 3 is that the number of Ganoderma lucidum in the components of the capsule content is 5 parts.

[0112] Experimental example

[0113] Experiment 1: Testing the anti-aging effect of the composition

[0114] Subjects: 550 healthy young male fruit flies were selected.

[0115] Feeding method: Drosophila were randomly divided into 11 groups, with 50 flies in each group. Samples were added to culture tubes according to the following formula.

[0116] Water control group: distilled water

[0117] Control group: tea oil

[0118] Experimental groups: The mice were fed with the capsule contents of Examples 1-8 and Comparative Examples 1-2, respectively.

[0119] Observe once in the morning and once in the evening every day, record the number of dead fruit flies in the culture tube, and then calculate the lifespan of the fruit flies. Compare the lifespan of each group, record the half-life time, maximum lifespan and average lifespan. The results are shown in Table 1.

[0120] The half-mortality time was determined as the time when half of the fruit flies in each group died;

[0121] Average lifespan = the sum of the survival time of each group of fruit flies / the number of fruit flies in the group;

[0122] The maximum lifespan was determined as the arithmetic mean of the lifespans of the last 10 flies that died in each group;

[0123] The extension rate is the ratio of the average lifespan of fruit flies, and its calculation formula is:

[0124] P X =L X -L0 / L0*100%;

[0125] The maximum lifespan extension rate is the extension ratio of the maximum lifespan of fruit flies, and its calculation formula is:

[0126] Q X =Lst X -Lst0 / Lst0*100%.

[0127] Table 1 Effects of samples on the lifespan of Drosophila

[0128]

[0129] As can be seen from Table 1, after feeding fruit flies with the contents of the capsules prepared in this scheme, the average lifespan of the fruit flies was significantly extended, indicating that the sample has an anti-aging effect; and after adjusting the formula, the average lifespan of the fruit flies was significantly reduced, indicating that the formula involved in this scheme has a good effect.

[0130] Experiment 2:

[0131] The present invention selected the sample of Example 4 for animal testing, and the testing process was as follows:

[0132] Subjects: 60 healthy adult mice were selected, 30 males and 30 females, weighing 18-22 g.

[0133] Feeding method: Mice were randomly divided into 6 groups, 10 in each group, and fed with the following formula once daily by gavage for 30 consecutive days: Water control group: distilled water 1g / (kg·d).

[0134] The control group was fed with 1 g of tea oil per kg of tea oil. The experimental groups were divided into three groups: the low-dose group was fed with 0.25 g of the sample from Example 4 per kg of tea oil per day, the medium-dose group was fed with 0.50 g of the sample from Example 4 per kg of tea oil per day, and the high-dose group was fed with 1.2 g of the sample from Example 4 per kg of tea oil per day.

[0135] 1. Detection of Antibody-producing Cells

[0136] After the animals were continuously immunized with defibrinated sheep red blood cells for 30 days, they were killed by cervical dislocation for 5 days, and the spleen was removed to prepare a spleen cell suspension with a cell concentration of 5 × 10 6 After dissolving the surface culture medium (1 g agarose in 100 mL of double-distilled water), heat and dissolve it in a 45°C water bath. Mix with an equal amount of twice-concentrated Hanks' solution at a pH of 7.2-7.4 and dispense into small test tubes, 0.5 mL per tube. Add 50 μL of 10% SRBC and 20 μL of spleen cell suspension to each tube, mix quickly, and pour onto a 6 cm plate coated with a thin layer of agar. After incubation for 1.5 h, count the number of hemolytic plaques. The results are listed in Table 2.

[0137] Table 2 Results of the effects of samples on the number of mouse antibody-producing cells

[0138]

[0139] As shown in Table 2, there are significant differences in the effects on the number of plaques among the water control group, the medium-dose group, and the high-dose group, indicating that the samples have the ability to increase the number of antibody-producing cells in mice.

[0140] 2. Determination of organ-to-body weight ratio:

[0141] After weighing, the mice were killed by cervical dislocation. The thymus and spleen were removed, the fascia was removed, the blood stains on the surface of the organs were absorbed with filter paper and weighed, and the thymus-to-body weight ratio was calculated. The measurement results are listed in Tables 3 and 4.

[0142] Table 3 Effects of samples on the ratio of thymus to body weight in mice

[0143]

[0144] It can be seen from Table 3 that there is no significant difference in the thymus-to-weight ratio of mice between different doses and the control group, so the samples have no significant effect on the thymus-to-weight ratio of animals.

[0145] Table 4 Effect of samples on mouse spleen weight ratio

[0146]

[0147] It can be seen from Table 4 that there is no significant difference in the spleen weight ratio of mice compared with the control group at different doses, so the samples have no obvious effect on the spleen weight ratio of animals.

[0148] 3.ConA-induced mouse spleen lymphocyte transformation test:

[0149] After 30 consecutive days of sampling, the animals were killed by cervical dislocation, and the spleen was removed to prepare a spleen cell suspension with a cell concentration of 3×10 6 / ml, the cell suspension was divided into two wells and added to a 24-well culture plate, 1 mL per well, 75 μL of ConA solution (equivalent to 7.5 μg / mL) was added to one well, and the other well was used as a control. The plate was placed in 5% CO2 and cultured at 37°C for 68 h. 4 h before the end of the culture, 0.7 mL of the supernatant was gently aspirated from each well, 0.7 mL of PRMI1640 culture medium without bovine serum was added, and 50 μL / well of MTT (5 mg / mL) was added. The culture was continued for 4 h. After the end of the culture, 1 mL of acidic isopropanol was added to each well and pipetted to mix until the purple crystals were completely dissolved. The liquid was then transferred to a 96-well plate, 3 parallel samples were added to each well, and the absorbance (A value) was measured at a wavelength of 570 nm using a microplate reader. The difference between the A value of the test well and the A value of the control well was calculated to indicate the proliferation ability of the lymphocytes. The test results are listed in Table 5.

[0150] Table 5 Effects of samples on ConA-induced mouse spleen lymphocyte transformation experiment results

[0151]

[0152] As shown in Table 5, there was no significant difference in the lymphocyte proliferation ability of mice among the water control group, solvent control group and the three dosage groups, indicating that the samples had no significant effect on improving the lymphocyte proliferation ability of mice.

[0153] 4. Carbon clearance test in mice:

[0154] After the animals were sampled for 30 consecutive days, 1:5 diluted India ink was injected into the tail vein, and the time was immediately measured after the ink was injected.

[0155] Ten minutes after the ink injection, 20 μL of blood was collected from the epicanthus venous plexus and added to 2 mL of sodium carbonate solution. The absorbance value was measured at a wavelength of 600 nm using a spectrophotometer, and the sodium carbonate solution was used as a blank control.

[0156] The phagocytic index was calculated based on the animal's body weight, liver weight, and spleen weight. The results were analyzed using analysis of variance and are listed in Table 6. If the serum hemolysin antibody count in the test group is higher than that in the control group, and the difference is significant, it can be determined that the test substance has the effect of enhancing the carbon clearance capacity of mouse monocytes and macrophages.

[0157] Table 6 Effects of samples on mouse serum hemolysin levels

[0158]

[0159] As shown in Table 6, there was no significant difference in the carbon clearance ability of mouse monocytes and macrophages among the water control group, solvent control group and the three dosage groups, indicating that the samples had no significant effect on the carbon clearance ability of mice.

[0160] 5. Mouse NK cell activity assay:

[0161] After 30 consecutive days of sampling, the animals were killed by cervical dislocation, and the spleen was removed to prepare a spleen cell suspension (effector cells). YAC-1 cells were taken 24 hours after passage and added with 1640 complete culture medium to adjust the cell concentration to 4×10 5 / mL (target cells), 100 μL of target cells and effector cells were taken at an effector-target ratio of 50:1 and added to a U-shaped 96-well culture plate. 100 μL of target cells and culture medium were added to the target cell natural release well, and 100 μL of target cells and 1% NP40 were added to the maximum release well. Each of the above items was set up with three replicates. The cells were cultured in a 37°C, 5% carbon dioxide incubator for 4 hours. 100 L of supernatant was aspirated from each well and placed in a flat-bottom 96-well culture plate. 100 μL of LDH matrix solution was added at the same time. The reaction was stopped for 3 minutes. 30 μL of 1 mol / L HCl was added to each well. The A value was measured at 490 nm using a microplate reader. The measurement results are listed in Table 7.

[0162] Table 7 Effects of samples on mouse NK cell activity

[0163]

[0164] As shown in Table 7, there was no significant difference in the activity of mouse NK cells among the water control group, solvent control group and the three dosage groups, indicating that the samples had no significant effect on the activity of mouse NK cells.

[0165] In summary, oral administration of samples in different dose groups to mice for 30 days had no adverse effect on the weight gain of mice, and had no effect on the weight ratio of the spleen and thymus of mice. The humoral immune function and cellular immune function test results of mice were all positive, while the monocyte-macrophage phagocytic ability and NK cell activity test results were all negative, indicating that the samples have the effect of enhancing the immune function.

[0166] The various embodiments in this specification are described in a progressive manner. Similar parts between the various embodiments can be referred to in conjunction with each other. Each embodiment focuses on the differences between the other embodiments. In particular, the system embodiments are generally similar to the method embodiments, so the description is relatively simple. For relevant parts, refer to the description of the method embodiments.

[0167] The foregoing is merely an embodiment of the present application, and the scope of protection of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modifications, equivalent substitutions, improvements, etc. made within the technical ideas and principles of the present application should be included in the scope of protection of the present application.

Claims

1. A tea oil health capsule for enhancing immunity and resisting aging, characterized in that: The capsule comprises a capsule skin and capsule contents. The capsule contents include the following components by weight: 20-40 parts of camellia oil, 12-20 parts of propolis, 8-10 parts of ganoderma lucidum, 8-10 parts of American ginseng, 7-9 parts of wolfberry, 7-9 parts of yam, 5-7 parts of pyrroloquinoline quinone, 5-7 parts of yeast, 5-7 parts of vitamin E, 4-6 parts of collagen peptide, and 4-6 parts of grape seed extract.

2. The immunity-enhancing and anti-aging tea oil health capsule according to claim 1, characterized in that: The mass ratio of Ganoderma lucidum to American ginseng, wolfberry and yam is 1:1:(0.75-0.8):(0.75-0.8).

3. The immunity-enhancing and anti-aging tea oil health capsule according to claim 1, characterized in that: The invention is prepared from the following components by weight: 30 parts of camellia oil, 16 parts of propolis, 9 parts of ganoderma lucidum, 9 parts of American ginseng, 8 parts of wolfberry, 8 parts of yam, 6 parts of pyrroloquinoline quinone, 6 parts of yeast, 6 parts of vitamin E, 5 parts of collagen peptide and 5 parts of grape seed extract.

4. The immunity-enhancing and anti-aging tea oil health capsule according to claim 1, characterized in that: The capsule skin comprises the following components by weight: 50-65 parts of sago starch, 33-35 parts of pectin, 20-23 parts of carrageenan, 5-10 parts of xanthan gum, 20-25 parts of glycerol, 1-3 parts of beta-glucan, 1-3 parts of citric acid, and 10-20 parts of purified water.

5. A method for preparing the immunity-enhancing and anti-aging tea oil health capsule according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Prepare camellia oil, yeast powder, Chinese medicine powder, propolis juice, capsule skin; S2. adding pyrroloquinoline quinone, dry yeast powder, vitamin E, collagen peptide, and grape seed extract to camellia oil to obtain a mixed camellia oil; S3. The herbal powder was thoroughly mixed with water and heated for 40-50min, then filtered, and propolis was added while hot, and mixed thoroughly to obtain a premix; S4 premixed liquid after cooling, add S3 obtained mixed camellia oil, mix thoroughly, that is, the contents of the capsule; S5. Filling the capsule contents into the capsule skin to prepare tea oil capsules.

6. The method for preparing the immunity-enhancing and anti-aging tea oil health capsule according to claim 5, characterized in that: The camellia oil is obtained by cold pressing camellia seeds and filtering them at a cold pressing temperature of 70-80° C. The Chinese medicinal powder is obtained by mechanically crushing ganoderma lucidum, American ginseng, wolfberry and yam and passing through a 650-750 mesh sieve.

7. The method for preparing the immunity-enhancing and anti-aging tea oil health capsule according to claim 5, characterized in that: In step S3, the heating pressure of the Chinese medicine powder is 140-160 kPa, and the heating temperature is 105-121°C.

8. The method for preparing the immunity-enhancing and anti-aging tea oil health capsule according to claim 5, characterized in that: The premixed solution in step S4 needs to be cooled to 20-30°C.

9. The method for preparing the immunity-enhancing and anti-aging tea oil health capsule according to claim 5, characterized in that: The preparation method of the capsule skin comprises the following steps: pre-swelling sago starch, pectin, carrageenan and xanthan gum in purified water, then heating and stirring to dissolve, adding glycerol, beta-glucan and citric acid, homogenizing and cooling, and adjusting the pH to 3.8 to obtain the capsule skin.

10. The method for preparing the immunity-enhancing and anti-aging tea oil health capsule according to claim 5, characterized in that: The pre-swelling conditions are swelling at 40-50°C for 30-40 minutes; secondary heating to 60-70°C, stirring speed 200-500rpm; homogenization speed 4000-5000rpm, homogenization for 10-15 minutes and then cooling to 40°C.

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