Composition capable of efficiently controlling oil, removing dandruff and relieving itching, shampoo and application thereof

Abstract

The invention provides a composition capable of efficiently controlling oil, removing dandruff and relieving itching, shampoo and application thereof. The composition is prepared from the following raw materials: verbena flower water, verbena oil, a mugwort extract, a radix gentianae extract, a southern semen cuscutae seed extract, a pepper fruit extract and a vitex trifolia fruit extract. The mass ratio of the verbena flower water to the verbena oil to the artemisia vulgaris extract to the radix gentianae extract to the southern semen cuscutae seed extract to the pepper fruit extract to the vitex trifolia fruit extract is (6-15): (1-3): (1-3): (1-3): (1-3): (1-3). The plant extract composition provided by the invention has a synergistic effect, has a better inhibition rate on hyaluronidase, has an inhibition effect close to that of positive control dimethyl glycyrrhizinate, has a stronger soothing effect, and is beneficial to guaranteeing scalp barriers. When the action time of the shampoo on malassezia furfur is 10 minutes, the bacteriostasis rate reaches 93.75%; the shampoo has an oil control function, the skin oil content can be reduced by 86.2% after the shampoo is used, and the effect is obvious.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and in particular to a high-efficiency oil-controlling, anti-dandruff and anti-itching composition, a shampoo and applications thereof. Background Art

[0002] Shampoo is used to cleanse the scalp and hair of body oils, sweat, sloughed cells, foreign dust, microorganisms, styling product residue, and unpleasant odors. With technological advancements, shampoos have added additional benefits such as repair, oil control, dandruff removal, itching relief, conditioning, and hair loss prevention. With the increasing availability of scalp care products and environmental influences, many consumers are experiencing issues such as excessive oil secretion and increased scalp sensitivity. Consequently, shampoos are trending towards using natural plant-based ingredients. China boasts a rich resource of traditional Chinese medicinal plants, and screening for effective compounds from these plants to achieve precise, stable, and side-effect-free effects is a crucial research path. By studying combinations of natural plant extracts, we have developed plant extract compositions with oil control, dandruff removal, and soothing properties. This provides consumers with natural and safe shampoos and provides a reference for the application of specialized functionalities in shampoo products. Summary of the Invention

[0003] In view of this, the purpose of the present invention is to propose a highly effective oil-control, anti-dandruff and anti-itching composition, shampoo and its application. By studying the combination of natural plant extracts, a plant extract composition with oil-control, anti-dandruff and soothing effects is obtained, which can provide consumers with natural and safe shampoo products and provide a reference basis for the application of the subdivided functions of hair care products.

[0004] The technical solution of the present invention is achieved as follows:

[0005] A highly effective oil-controlling, anti-dandruff and anti-itching composition comprising the following raw materials: verbena flower water, verbena oil, Artemisia vulgaris extract, Gentiana scabra extract, Cuscuta australis seed extract, Zanthoxylum bungeanum fruit extract, and Vitex rotundifolia fruit extract;

[0006] The mass ratio of the verbena flower water, verbena oil, Artemisia annua extract, Gentiana scabra extract, Cuscuta australis seed extract, Zanthoxylum bungeanum fruit extract and Vitex rotundifolia fruit extract is 6-15:1-3:1-3:1-3:1-3:1-3:1-3:1-3.

[0007] A further solution is that the mass ratio of the verbena flower water, verbena oil, Artemisia selengensis extract, Gentiana scabra extract, Cuscuta australis seed extract, Zanthoxylum bungeanum fruit extract and Vitex rotundifolia fruit extract is 10-15:1-2:1-2:1-2:1-2:1-2:1-2:1-2.

[0008] A further solution is that the preparation method of the verbena flower water comprises: taking verbena flowers, drying and crushing them, adding water at a material-liquid mass ratio of 1:15-25, mixing, soaking and extracting at 80-90° C. for 4-8 hours, cooling to room temperature, performing enzymatic extraction at 40-50° C. for 20-40 minutes at a verbena and cellulase mass ratio of 1:0.006-0.01, inactivating the enzyme, filtering, and collecting the filtrate to obtain the verbena flower water;

[0009] The preparation method of verbena oil comprises the following steps: taking verbena flowers, drying and crushing them, adding water to the mixture at a material-liquid mass ratio of 1:15-25, soaking and extracting at 80-90° C. for 4-8 hours, cooling to room temperature, performing enzymatic extraction at 40-50° C. for 20-40 minutes at a verbena and cellulase mass ratio of 1:0.006-0.01, steam distilling the enzymatic hydrolyzate, collecting the distillate, and separating the oil and water to obtain verbena oil.

[0010] In a specific embodiment of the present invention, the preparation method of verbena flower water is as follows: verbena flowers are taken, dried and crushed, and then 20 times the weight of the raw materials are added with water and mixed. The mixture is soaked and extracted at 80-90° C. for 6 hours, cooled to room temperature, and cellulase is added in an amount of 0.8% by weight of the raw materials. The mixture is enzymatically hydrolyzed and extracted at 40-50° C. for 0.5 hours. After the enzyme is inactivated, the mixture is filtered (with a 0.05 μm filter membrane) to collect the filtrate to obtain verbena (VERBENA OFFICINALIS) flower water; the enzymatic hydrolyzate is subjected to steam distillation, the distillate is collected, and the oil-water separation is performed to obtain verbena (VERBENA OFFICINALIS) oil.

[0011] A further solution is that the preparation method of the Artemisia annua extract or the Gentiana scabra extract includes: taking the whole herb of Artemisia annua or the whole herb of Gentiana scabra, drying and crushing it, adding a volume concentration of 75%-85% ethanol solution at a material-liquid mass ratio of 1:6-10, ultrasonically extracting at 70-80°C and 300W-400 for 2-5h, filtering, collecting the filtrate, and freeze-drying to obtain the Artemisia annua extract or the Gentiana scabra extract.

[0012] In a specific embodiment of the present invention, the preparation method of Artemisia vulgaris extract or Gentiana scabra extract is: take the whole herb of Artemisia vulgaris or the whole herb of Gentiana scabra, dry and crush it, add 80% ethanol solution with a volume concentration of 8 times the weight of the raw material, perform ultrasonic extraction at 360W for 3 hours at 70-80°C, filter, collect the filtrate, and freeze-dry to obtain Artemisia vulgaris extract or Gentiana scabra extract.

[0013] A further solution is that the preparation method of the southern dodder seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex rotundum fruit extract includes: taking the southern dodder seed, the Zanthoxylum bungeanum fruit, or the vitex rotundum fruit, crushing them, adding a volume concentration of 75%-85% ethanol solution at a material-liquid mass ratio of 1:15-25, reflux extraction 2-4 times, each extraction for 1-2 hours, filtering, collecting and combining the filtrate, and recovering ethanol to obtain the southern dodder seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex rotundum fruit extract.

[0014] In a specific embodiment of the present invention, the preparation method of the southern dodder seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex trifolia fruit extract is as follows: the southern dodder (CUSCUTA AUSTRALIS) seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex trifolia fruit extract is obtained by reflux extraction with an 80% by volume ethanol solution. The specific preparation method is as follows: southern dodder seeds, the Zanthoxylum bungeanum fruit, or the vitex trifolia fruit are taken, crushed, and an 80% by volume ethanol solution of 20 times the weight of the raw materials is added, refluxed and extracted three times, each extraction for 1 hour, filtered, the combined filtrate is collected, and ethanol is recovered to obtain the southern dodder (CUSCUTA AUSTRALIS) seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex trifolia fruit extract.

[0015] A high-efficiency oil-controlling, anti-dandruff and anti-itching shampoo is prepared from the high-efficiency oil-controlling, anti-dandruff and anti-itching composition.

[0016] A further solution is that, by weight percentage, the amount of the high-efficiency oil-controlling, anti-dandruff and anti-itching composition added to the shampoo is 8%-15%.

[0017] Furthermore, the present invention provides the use of the above-mentioned high-efficiency oil-controlling, anti-dandruff and antipruritic composition in the preparation of products that inhibit hyaluronidase.

[0018] Furthermore, the present invention provides the use of the above-mentioned high-efficiency oil-controlling, anti-dandruff and anti-itching composition in the preparation of a product for reducing the oil content of the scalp.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The present invention selects verbena (VERBENA OFFICINALIS) floral water, verbena (VERBENA OFFICINALIS) oil, artemisia vulgaris extract, gentian (GENTIANA SCABRA) extract, southern dodder (CUSCUTA AUSTRALIS) seed extract, zanthoxylum bungeanum fruit extract and vitex trifolia fruit extract to have a synergistic effect, have a better inhibition rate on hyaluronidase, and the inhibition effect is close to that of the positive control dimethyl glycyrrhizate, have a stronger soothing effect, and help protect the scalp barrier.

[0021] In the Malassezia inhibition test, the shampoo prepared from the above composition has anti-dandruff and antipruritic effects. When the shampoo acts on Malassezia furfur for 10 minutes, it shows that the product has a strong antibacterial effect. When the action time is 15 minutes, the antibacterial rate reaches 97.28%.

[0022] In the oily skin test, the shampoo prepared from the above composition has an oil-control function. After using the shampoo of the present invention, the oil content of the skin can be reduced by 86.2%, and the effect is obvious.

[0023] The composition provided by the present invention is composed of various plant extracts. The extraction method of the plant extracts is simple, the conditions are mild, and there is no irritation or sensitization. The prepared product has good safety and easy quality control. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0026] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0027] Malassezia furfur ATCC44344 was purchased from Guangdong Provincial Microbiological Culture Collection Center.

[0028] Example 1-

[0029] Oil-control, anti-dandruff and anti-itching shampoo - formula table

[0030]

[0031] The preparation method of verbena (VERBENA OFFICINALIS) floral water is as follows: verbena flowers are dried and crushed, and then 20 times the weight of water of the raw materials are added and mixed, and the mixture is soaked and extracted at 80-90° C. for 6 hours, cooled to room temperature, and cellulase in an amount of 0.8% by weight of the raw materials is added, and enzymatic extraction is carried out at 40-50° C. for 0.5 hours. After inactivation of the enzyme, the mixture is filtered (0.05 μm filter membrane), and the filtrate is collected to obtain verbena (VERBENA OFFICINALIS) floral water.

[0032] The preparation method of verbena (VERBENA OFFICINALIS) oil comprises the following steps: taking verbena flowers, drying and crushing them, adding 20 times the weight of the raw material in water, mixing, soaking and extracting at 80-90°C for 6 hours, cooling to room temperature, adding cellulase in an amount of 0.8% by weight of the raw material, performing enzymatic extraction at 40-50°C for 0.5 hour, steam distilling the enzymatic hydrolyzate, collecting the distillate, and separating the oil and water to obtain verbena (VERBENA OFFICINALIS) oil.

[0033] Artemisia vulgaris extract / Gentiana scabra extract is obtained by extraction with an 80% volume concentration ethanol solution. The specific preparation method is: take the whole herb of Artemisia vulgaris / Gentiana scabra, dry and crush it, add an 80% volume concentration ethanol solution 8 times the weight of the raw material, extract it at 360W ultrasonically at 70-80°C for 3h, filter, collect the filtrate, and freeze-dry to obtain the Artemisia vulgaris extract / Gentiana scabra extract.

[0034] Cuscuta australis seed extract / Zanthoxylum bungeanum fruit extract / Vitex trifolia fruit extract are obtained by reflux extraction with an 80% by volume ethanol solution. The specific preparation method is as follows: southern dodder seeds / Zanthoxylum bungeanum fruits / Vitex trifolia fruits are crushed, and an 80% by volume ethanol solution with a concentration of 20 times that of the raw materials is added. The mixture is refluxed and extracted three times for 1 hour each time. The mixture is filtered, the combined filtrate is collected, and the ethanol is recovered to obtain the Cuscuta australis seed extract / Zanthoxylum bungeanum fruit extract / Vitex trifolia fruit extract.

[0035] Test Example 1-

[0036] Hyaluronidase inhibition test

[0037] Test sample test table (weight ratio)

[0038]

[0039] Hyaluronic acid is associated with skin moisturizing and firming, and the products of its synthesis and metabolism are involved in immune regulation. Studies have found that hyaluronic acid degradation products of different molecular weights can promote inflammatory responses. Hyaluronidase inhibition tests can be used to evaluate the soothing effect of a sample; that is, a higher hyaluronidase inhibition rate indicates a stronger soothing effect.

[0040] Samples 1 to 6 were diluted with pure water to a mass concentration of 10% and used as test samples, and dimethyl glycyrrhizinate was diluted with pure water to a mass concentration of 3% and used as a positive control.

[0041] The Elson-Morgan modified method was used for determination, with three parallel assays. Color development was carried out at room temperature for 30 min, and the absorbance at a wavelength of 528 nm was measured using an ultraviolet spectrophotometer. The inhibition rate was calculated according to the following formula.

[0042] Inhibition rate / % = [(T1-T2)-(T3-T4)] / (T1-T2)×100%

[0043] Wherein, T1 is the absorbance value of the control solution without the sample to be tested, T2 is the absorbance value of the control blank without the sample to be tested and the enzyme, T3 is the absorbance value of the sample solution containing the sample to be tested, and T4 is the absorbance value of the sample blank containing the sample to be tested and the enzyme.

[0044] The test results are as follows:

[0045] Table 1 Hyaluronidase inhibition rate (%)

[0046] Group Inhibition rate Sample 1 46.27 Sample 2 41.33 Sample 3 34.53 Sample 4 35.80 Sample 5 31.07 Sample 6 33.68 Positive control 56.14

[0047] As can be seen from Table 1 above, the inhibition rates of different combinations of plant extracts on hyaluronidase are different. Among them, the inhibition rate of sample 1 on hyaluronidase reaches 46.27%, which is closer to the positive control, indicating that the combination of plant extracts has a stronger soothing effect; compared with sample 1, the inhibition rates of samples 3 to 6 are all reduced to varying degrees, indicating that the present invention uses verbena (VERBENA OFFICINALIS) flower water, verbena (VERBENA OFFICINALIS) oil, northern wormwood (ARTEMISIA VULGARIS) extract, gentian (GENTIANASCABRA) extract, southern dodder (CUSCUTA AUSTRALIS) seed extract, pepper (ZANTHOXYLUM BUNGEANUM) fruit extract and vitex (VITEX TRIFOLIA) fruit extract to have a synergistic effect.

[0048] Test Example 2-

[0049] Malassezia inhibition test

[0050] According to the instructions for bacterial subculture, Malassezia furfur was activated and subcultured twice using LN medium, and the number of viable bacteria was about 10 6 cfu / mL, centrifuged, washed with PBS, and prepared into a bacterial suspension. According to the appendix of GB15979-2002 "Hygienic Standards for Disposable Sanitary Products", the inhibition rate of the shampoo in Example 1 on Malassezia furfur was tested by colony counting method.

[0051] Inhibition rate / %=(number of colonies in the control sample - number of colonies in the test sample) / number of colonies in the control sample × 100%.

[0052] Evaluation criteria: If the antibacterial rate is ≥50% to 90%, it indicates that the product has an antibacterial effect; if the antibacterial rate is ≥90%, it indicates that the product has a strong antibacterial effect.

[0053] The test results are as follows:

[0054] Table 2 Inhibition rate of Malassezia furfur (%)

[0055] time Antibacterial rate 2min 70.33 5min 85.21 10min 93.75 15min 97.28

[0056] As can be seen from Table 2 above, as time goes by, the inhibitory effect of shampoo on Malassezia furfur becomes stronger. When the action time of shampoo on Malassezia furfur is 5 minutes, it is shown that the product has a certain antibacterial effect. When the action time of shampoo on Malassezia furfur is 10 minutes, the product has a strong antibacterial effect. When the action time is 15 minutes, the antibacterial rate reaches 97.28%, indicating that the shampoo prepared by selecting verbena (VERBENA OFFICINALIS) flower water, verbena (VERBENA OFFICINALIS) oil, artemisia vulgaris extract, gentian (GENTIANA SCABRA) extract, southern dodder (CUSCUTA AUSTRALIS) seed extract, ZANTHOXYLUM BUNGEANUM fruit extract and Vitex trifolia (VITEX TRIFOLIA) fruit extract has good anti-dandruff and antipruritic effects.

[0057] Test Example 3-

[0058] Scalp oil test

[0059] Recruit 20 volunteers with oily skin, aged 20 to 30 years old, who meet the inclusion criteria, and undergo skin testing according to the "Human Skin Patch Test" of the "Technical Specifications for Safety of Cosmetics". Those who do not show adverse reactions will undergo a scalp oil test.

[0060] The volunteers were divided into left and right parts along the hairline. One half of the head was washed with water, and the other half was washed with the shampoo in Example 1. The volunteers washed their hair on both sides and dried it by themselves until the hair was not dripping. The volunteers sat quietly in the observation room 30 minutes before the test point. The temperature in the observation room was set at 22-25°C and the relative humidity was 55%-65%. MB560 Scalp Oil Tester measures the oil content of the scalp. The test area is the area from the forehead to 3 cm on the top of the head.

[0061] The test results are as follows:

[0062] Table 3 Scalp oil content (μg / cm 2 )

[0063] Group Before use Completed 5h after use 10 hours after use Qingshui District 467.0±117.2 311.8±93.9 368.0±127.7 386.2±124.6 Sample Area 482.6±106.5 66.7±24.5 174.6±124.3 331.6±118.8

[0064] Note: "Complete use" means testing 30 minutes after hair is dried

[0065] Scalp oil secretion is a dynamic process. As shown in Table 3 above, the scalp oil content gradually increases with time after shampooing. After 20 volunteers used the sample, the skin oil content in the clear water area and the sample area decreased by 33.2% and 86.2%, respectively, compared with before use. 5 hours after use, the skin oil content in the clear water area and the sample area decreased by 21.2% and 63.8%, respectively, compared with before use. 10 hours after use, the skin oil content in the clear water area and the sample area decreased by 17.3% and 31.3%, respectively, compared with before use. It can be seen that the shampoo prepared by the present invention has an oil-control function with obvious effect.

[0066] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A highly effective oil-controlling, anti-dandruff and antipruritic composition, characterized in that: Ingredients include: verbena flower water, verbena oil, artemisia annua extract, gentiana scabra extract, dodder seed extract, zanthoxylum bungeanum fruit extract, and vitex rotundifolia fruit extract; The mass ratio of the verbena flower water, verbena oil, Artemisia annua extract, Gentiana scabra extract, Cuscuta australis seed extract, Zanthoxylum bungeanum fruit extract and Vitex rotundifolia fruit extract is 6-15:1-3:1-3:1-3:1-3:1-3:1-3:1-3.

2. The high-efficiency oil-controlling, anti-dandruff and antipruritic composition according to claim 1, characterized in that: The mass ratio of the verbena flower water, verbena oil, Artemisia annua extract, Gentiana scabra extract, Cuscuta australis seed extract, Zanthoxylum bungeanum fruit extract and Vitex rotundifolia fruit extract is 10-15:1-2:1-2:1-2:1-2:1-2:1-2.

3. A highly effective oil-controlling, anti-dandruff and antipruritic composition according to claim 1 or 2, characterized in that: The preparation method of verbena flower water comprises: taking verbena flowers, drying and crushing them, adding water at a material-liquid mass ratio of 1:15-25, mixing, soaking and extracting at 80-90° C. for 4-8 hours, cooling to room temperature, performing enzymatic extraction at 40-50° C. for 20-40 minutes at a verbena and cellulase mass ratio of 1:0.006-0.01, inactivating the enzyme, filtering, and collecting the filtrate to obtain verbena flower water; The preparation method of verbena oil comprises the following steps: taking verbena flowers, drying and crushing them, adding water to the mixture at a material-liquid mass ratio of 1:15-25, soaking and extracting at 80-90° C. for 4-8 hours, cooling to room temperature, performing enzymatic extraction at 40-50° C. for 20-40 minutes at a verbena and cellulase mass ratio of 1:0.006-0.01, steam distilling the enzymatic hydrolyzate, collecting the distillate, and separating the oil and water to obtain verbena oil.

4. A highly effective oil-controlling, anti-dandruff and antipruritic composition according to claim 1 or 2, characterized in that: The preparation method of the Artemisia annua extract or the Gentiana scabra extract includes: taking the whole Artemisia annua herb or the whole Gentiana scabra herb, drying and crushing it, adding a 75%-85% ethanol solution by volume at a material-liquid mass ratio of 1:6-10, ultrasonically extracting at 70-80°C and 300W-400 for 2-5 hours, filtering, collecting the filtrate, and freeze-drying to obtain the Artemisia annua extract or the Gentiana scabra extract.

5. A highly effective oil-controlling, anti-dandruff and anti-itching composition according to claim 1 or 2, characterized in that: The preparation method of the southern dodder seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex rotundum fruit extract comprises: taking the southern dodder seed, the Zanthoxylum bungeanum fruit, or the vitex rotundum fruit, crushing the same, adding an ethanol solution with a volume concentration of 75% to 85% at a material-liquid mass ratio of 1:15-25, performing reflux extraction for 2-4 times, each extraction taking 1-2 hours, filtering, collecting and combining the filtrate, and recovering ethanol to obtain the southern dodder seed extract, the Zanthoxylum bungeanum fruit extract, or the vitex rotundum fruit extract.

6. An efficient oil-controlling, anti-dandruff and anti-itching shampoo, characterized in that: The invention is prepared from the high-efficiency oil-controlling, anti-dandruff and anti-itching composition according to any one of claims 1 to 5.

7. The high-efficiency oil-controlling, anti-dandruff and anti-itching shampoo according to claim 6, characterized in that: Calculated by weight percentage, the addition amount of the high-efficiency oil-controlling, anti-dandruff and anti-itching composition in shampoo is 8%-15%.

8. Use of the high-efficiency oil-controlling, anti-dandruff and antipruritic composition according to any one of claims 1 to 5 in the preparation of a product for inhibiting hyaluronidase.

9. Use of the high-efficiency oil-controlling, anti-dandruff and anti-itching composition according to any one of claims 1 to 5 in the preparation of a product for reducing scalp oil content.

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