Anti-aging, anti-wrinkle and anti-tumor Chinese yam polysaccharide composition and preparation method thereof

Abstract

The invention discloses an anti-aging, anti-wrinkle and anti-tumor Chinese yam polysaccharide composition and a preparation method thereof. The composition comprises the following components in parts by mass: 30-80 parts of Chinese yam polysaccharide, 10-50 parts of stachyose, 5-25 parts of pomegranate seed extract, 5-20 parts of grape seed extract, 3-15 parts of blueberry extract, 2-12 parts of tea polyphenol, 1-10 parts of sea cucumber peptide and 1-10 parts of soybean peptide (with the molecular weight of 300-1000Da). Preferably, 0.5-5 parts of resveratrol (the purity is greater than or equal to 98%) and 3-20 parts of acerola cherry powder (VC is greater than or equal to 15%) are added. The preparation method comprises the following steps: dissolving polysaccharides and saccharides, adding the extract, homogenizing, adjusting the pH value to 6.0-7.0, carrying out enzymolysis by using compound protease at 35-40 DEG C, and finally carrying out spray drying. Experiments show that the composition has a synergistic effect in the aspects of resisting saccharification (the highest inhibition rate reaches 78.5%), resisting oxidation (the DPPH clearance rate is 91.3%), increasing the content of hydroxyproline in skin (+ 74.1%), inhibiting tumor cell proliferation (62.5%) and improving ultraviolet wrinkles (the score is reduced by 61%).

Description

Technical Field

[0001] The invention belongs to the field of natural active compositions, and specifically discloses an anti-aging, anti-wrinkle and anti-tumor yam polysaccharide composition and a preparation method thereof. Background Art

[0002] Current functional products in the anti-aging and skin health sectors face common technical bottlenecks. On the one hand, while plant-based active ingredients (such as polyphenols and polysaccharides) possess antioxidant potential, their large molecular weight hinders effective absorption, resulting in insufficient bioavailability. On the other hand, while small-molecule peptides facilitate penetration, they struggle to simultaneously achieve multiple benefits, such as anti-glycation and cytoprotection. Existing technologies typically employ two approaches: one is to increase the diversity of active ingredients through the use of complex plant extracts, but this fails to address the absorption barriers caused by excessive molecular weight. The other is to enhance efficacy through chemical modification or synthetic additives, which introduces safety risks. A further significant challenge lies in the fundamentally different process requirements for key active ingredients (e.g., anti-tumor polysaccharides require a molecular weight of 10-50 kDa, while anti-wrinkle peptides require a molecular weight of ≤1500 Da). Conventional single-processing techniques struggle to achieve both activity retention and precise molecular weight control. Therefore, developing a natural combination that simultaneously improves absorption efficiency, stability, and synergizes multiple benefits requires overcoming the dual technical barriers of ingredient composition and processing technology. Summary of the Invention

[0003] In order to solve the above-mentioned problems in the prior art, the present invention discloses an anti-aging, anti-wrinkle and anti-tumor yam polysaccharide composition and a preparation method thereof.

[0004] To achieve the above objectives, the present invention includes the following technical solutions.

[0005] A yam polysaccharide composition, comprising, by mass, 30-80 parts of yam polysaccharide, 10-50 parts of stachyose, 5-25 parts of pomegranate seed extract, 5-20 parts of grape seed extract, 3-15 parts of blueberry extract, 2-12 parts of tea polyphenols, 1-10 parts of sea cucumber peptide, and 1-10 parts of soybean peptide. In this solution, the four-dimensional combination of polysaccharide (yam) - oligosaccharide (stachyose) - polyphenol (pomegranate / grape / blueberry) - peptide (sea cucumber / soybean) is used to solve the problems of low absorption efficiency (large molecular weight difference) and single efficacy of traditional anti-aging ingredients. Experiments in some other embodiments show that this ratio is significantly superior to the control group lacking key ingredients in terms of anti-glycation, anti-oxidation, collagen regeneration and anti-tumor.

[0006] Furthermore, the above composition also includes 0.5-5 parts resveratrol and 3-20 parts acerola cherry powder. In this solution, the addition of high-purity resveratrol and acerola cherry powder (high in VC) forms a synergistic system for photodamage repair. Data from some embodiments demonstrate that resveratrol significantly enhances anti-glycation effects, acerola cherry powder enhances UV-induced wrinkle improvement, and the combined use of the two improves DPPH scavenging.

[0007] Furthermore, in the aforementioned composition, the ellagic acid content of the pomegranate seed extract is ≥40%, the proanthocyanidin content of the grape seed extract is ≥95%, and the anthocyanidin content of the blueberry extract is ≥25%. In this protocol, the ellagic acid content is limited to ≥40% (pomegranate seed), ≥95% (grape seed), and ≥25% (blueberry) to ensure the concentration of the core active ingredients. In some embodiments, omitting the pomegranate / grape seed extracts results in an anti-glycation inhibition rate of only 24.6%, while the inhibition rate after combining the extracts meeting the standards is >65.8%, demonstrating that active content is the key factor in efficacy differences.

[0008] Furthermore, in the above-mentioned composition, the average molecular weight of the sea cucumber peptide is 500-1500Da, the average molecular weight of the soybean peptide is 300-1000Da, and the average molecular weight of the yam polysaccharide is 10-50kDa. The present invention uses the molecular weight range of yam polysaccharide (10-50kDa), sea cucumber peptide (500-1500Da), and soybean peptide (300-1000Da) to solve the contradiction between the absorption of large molecules and the synergistic effect of small molecules. In the test example, the anti-glycation inhibition rate of Comparative Example 3 (replacing yam polysaccharide with maltodextrin) dropped to 28.5% (Test Example 1), which proves the necessity of precise control of molecular weight.

[0009] Furthermore, the resveratrol in the above composition has a purity of ≥98%, and the vitamin C content in the acerola cherry powder is ≥15%. This solution requires a resveratrol purity of ≥98% and acerola cherry powder VC content of ≥15% to prevent impurities from interfering with the active ingredients. Experiments in test cases showed that the combination of high-purity resveratrol and high-VC acerola cherry powder achieved antioxidant (scavenging rate 91.3%) and anti-wrinkle (collagen area ratio 68.3%) effects close to those of the positive control group (VC 94.6% / retinoic acid 73.8%).

[0010] Furthermore, the above composition includes, by mass: 50 parts of yam polysaccharide, 30 parts of stachyose, 15 parts of pomegranate seed extract, 10 parts of grape seed extract, 8 parts of blueberry extract, 6 parts of tea polyphenols, 5 parts of sea cucumber peptide, and 4 parts of soybean peptide.

[0011] Furthermore, the above composition also includes 2 parts of resveratrol and 12 parts of acerola cherry powder.

[0012] The present invention also discloses a method for preparing the composition, comprising the following steps:

[0013] (1) Mix yam polysaccharide and stachyose, add 5 to 10 times the mass of water, and stir to dissolve at 50 to 60°C;

[0014] (2) adding pomegranate seed extract, grape seed extract, blueberry extract and tea polyphenols and homogenizing for 10 to 30 minutes;

[0015] (3) adding sea cucumber peptide and soybean peptide, adjusting the pH to 6.0-7.0, and performing enzymatic hydrolysis at 35-40°C for 1-2 h;

[0016] (4) When resveratrol is present, resveratrol is added after enzymatic hydrolysis;

[0017] (5) When acerola cherry powder is present, add the acerola cherry powder and mix;

[0018] (6) After vacuum concentration, spray drying is performed with an air inlet temperature of 100-115°C and an air outlet temperature of 65-85°C.

[0019] The above preparation method, as demonstrated in some test cases, utilizes a step-by-step process: ① dissolution at 50-60°C (to preserve the activity of heat-sensitive polysaccharides) → ② homogenization (to improve polyphenol dispersion) → ③ enzymatic hydrolysis at 35-40°C (to target peptide activation) → ④ post-addition of resveratrol (to avoid high-temperature inactivation) → ⑤ spray drying (inlet air temperature ≤ 115°C to ensure VC retention). In Comparative Example 5 (enzymatic hydrolysis at 50°C), the anti-glycation inhibition rate dropped to 41.2% (Test Example 1), demonstrating the necessity of low-temperature processing.

[0020] Furthermore, in the above preparation method, the enzymatic hydrolysis in step (3) adopts a composite protease, and the enzymatic hydrolysis conditions are:

[0021] The added amount of the composite protease is 0.1-0.5 wt %, the enzymatic hydrolysis pH is 6.2-6.8, and the enzymatic hydrolysis time is 90-120 min.

[0022] From some test examples, it can be seen that limiting the composite protease to 0.1-0.5wt%, pH 6.2-6.8, and time 90-120 minutes achieves efficient peptide activation. Data show that under these conditions, peptide efficacy is maximized. Comparative Example 1, which lacks enzymatic hydrolysis, only increases hydroxyproline by 13.3%, while after optimized enzymatic hydrolysis (Example 2), it reaches 47.6%.

[0023] The invention also discloses the use of the composition in preparing health products, functional foods or skin repair preparations.

[0024] Compared with the prior art, the present invention has the following outstanding beneficial effects:

[0025] This invention, through the synergistic effect of specific molecular weight compatibility and step-by-step enzymatic hydrolysis process, breaks through the technical bottleneck of low absorption efficiency of active ingredients and difficulty in balancing multiple functions in traditional anti-aging products. Specifically, it is manifested as:

[0026] 1. Synergistic enhancement of efficacy: It integrates five functions: anti-glycation (experimental inhibition rate up to 78.5%), antioxidant (DPPH clearance rate 91.3%), collagen regeneration (hydroxyproline +74.1%), anti-tumor (cancer cell inhibition rate 62.5%), and anti-wrinkle (UV wrinkle score reduced by 61%), especially for the simultaneous improvement of photoaging and natural aging;

[0027] 2. Doubled biological efficacy: Small molecule peptides and precisely extracted polyphenols / anthocyanidins form a mutually promoting absorption system, significantly improving oral bioavailability;

[0028] 3. Safe and widely applicable: All natural ingredients are practically non-toxic after acute toxicity verification (LD 50 >5000mg / kg), suitable for oral health products, functional foods and external skin repair preparations;

[0029] 4. Stable and controllable process: The homogenization-low-temperature enzymatic hydrolysis-gradient drying process ensures the activity of heat-sensitive ingredients (such as resveratrol and VC). The water solubility of the spray-dried finished product is greater than 98%, which is convenient for industrial application. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 Comparison of AGE inhibition rate (%) at 28 days in the anti-glycation end products (AGEs) inhibition experiment;

[0031] Figure 2 Comparison of DPPH scavenging rate (%) in DPPH free radical scavenging experiment;

[0032] Figure 3 Comparison of hydroxyproline content in mouse skin hydroxyproline content assay;

[0033] Figure 4 MCF-7 breast cancer cell proliferation inhibition test results. DETAILED DESCRIPTION

[0034] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0035] Table 1: Raw materials

[0036] Raw material name Technical Specifications Yam polysaccharides Average molecular weight 25kDa, purity ≥80% Stachyose Content ≥85%, oligosaccharide tetrasaccharide ≥95% Pomegranate seed extract Ellagic acid ≥40%, total polyphenols ≥60% grape seed extract Proanthocyanidins ≥95%, degree of polymerization 2-4 blueberry extract Anthocyanin ≥ 25% (calculated as cyanidin-3-glucoside) Tea polyphenols EGCG ≥ 60%, total polyphenols ≥ 98% Sea Cucumber Peptide Average molecular weight 1000Da, peptide content ≥90% Soy peptide Average molecular weight 500Da, solubility ≥95% Resveratrol Trans configuration ≥98%, purity ≥99% Acerola powder Natural vitamin C ≥ 15%, total flavonoids ≥ 5%

[0037] Table 2: Auxiliary table

[0038] Raw material name use Add Scale Specifications Complex protease Directed enzymatic hydrolysis of peptides 0.1-0.5% of the total weight of raw materials Enzyme activity ≥50,000U / g maltodextrin Spray drying additives 10-30% total solids DE value 15-20 citric acid pH adjuster (enzymatic hydrolysis stage) Adjust pH to 6.0-7.0 Food grade ≥99.5%

[0039] Example 1

[0040] A yam polysaccharide composition with anti-aging, anti-wrinkle and anti-tumor effects is prepared from the following raw materials by mass fraction:

[0041] Yam polysaccharide 30 parts, stachyose 50 parts, pomegranate seed extract 25 parts, grape seed extract 5 parts, blueberry extract 15 parts, tea polyphenol 2 parts, sea cucumber peptide 10 parts, and soybean peptide 1 part.

[0042] The preparation method comprises:

[0043] (1) Mix the yam polysaccharide and stachyose, and add 5 times the mass of water, and stir and dissolve at 50°C;

[0044] (2) Add the pomegranate seed extract, grape seed extract, blueberry extract, and tea polyphenol, and homogenize for 30 min;

[0045] (3) Add the sea cucumber peptide and soybean peptide, adjust the pH to 6.0, and enzymatically hydrolyze at 40°C for 1 h (add 0.1% of complex protease);

[0046] (4) After vacuum concentration, spray dry (inlet temperature 100°C, outlet temperature 65°C).

[0047] Example 2

[0048] Comprise, by mass fraction:

[0049] Yam polysaccharide 50 parts, stachyose 30 parts, pomegranate seed extract 15 parts, grape seed extract 10 parts, blueberry extract 8 parts, tea polyphenol 6 parts, sea cucumber peptide 5 parts, and soybean peptide 4 parts.

[0050] The preparation method comprises:

[0051] (1) After mixing the raw materials, add 8 times the mass of water, and stir and dissolve at 55°C;

[0052] (2) Homogenize for 20 min;

[0053] (3) Adjust the pH to 6.5, and enzymatically hydrolyze at 37°C for 1.5 h (0.3% of complex protease);

[0054] (4) Spray dry (inlet temperature 105°C, outlet temperature 75°C).

[0055] The other steps are the same as in Example 1.

[0056] Example 3

[0057] Comprise, by mass fraction:

[0058] 80 parts of yam polysaccharide, 10 parts of stachyose, 5 parts of pomegranate seed extract, 20 parts of grape seed extract, 3 parts of blueberry extract, 12 parts of tea polyphenols, 1 part of sea cucumber peptide, and 10 parts of soybean peptide.

[0059] The preparation method is the same as Example 2.

[0060] Example 4

[0061] Based on the formula of Example 2, 2 parts of resveratrol were added and the preparation method was adjusted as follows:

[0062] (3) After enzymatic hydrolysis, resveratrol was added, and the remaining steps were the same as in Example 2.

[0063] Example 5

[0064] Based on the formula of Example 2, 2 parts of resveratrol and 12 parts of acerola cherry powder were added, and the preparation method was adjusted as follows:

[0065] (3) adding resveratrol after enzymatic hydrolysis;

[0066] (4) Add acerola cherry powder and mix;

[0067] (5) Spray drying (inlet air 110℃, outlet air 85℃).

[0068] Comparative Example 1

[0069] Based on the formula of Example 2, sea cucumber peptide and soybean peptide were deleted, and the rest was the same as Example 2.

[0070] Comparative Example 2

[0071] Based on the formula of Example 2, tea polyphenols were deleted, and the rest was the same as Example 2.

[0072] Comparative Example 3

[0073] Based on the formula of Example 2, yam polysaccharide was replaced with an equal amount of maltodextrin, and the rest was the same as Example 2.

[0074] Comparative Example 4

[0075] Based on the formula of Example 2, the pomegranate seed extract and grape seed extract were deleted, and the rest was the same as in Example 2.

[0076] Comparative Example 5

[0077] Based on the formula of Example 2, the enzymatic hydrolysis temperature was increased to 50° C., and the rest was the same as Example 2.

[0078] Test Example 1

[0079] Anti-glycation end products (AGEs) inhibition experiment

[0080] Objective: To verify the synergistic effect of the combination in inhibiting protein glycosylation

[0081] method:

[0082] Model construction:

[0083] Reaction system: bovine serum albumin (BSA, 50 mg / mL) + glucose (0.5 M) + 0.02% NaN3 (antibacterial).

[0084] Add samples: aqueous solutions of Examples 1-5 and Comparative Examples 1-5 (1 mg / mL), and positive control aminoguanidine (1 mg / mL).

[0085] Incubate at 37°C in the dark for 28 days and take samples weekly.

[0086] Detection method:

[0087] Fluorescence detection of AGEs: excitation wavelength 370 nm, emission wavelength 440 nm.

[0088] Inhibition rate = (fluorescence value of the control group - fluorescence value of the experimental group) / fluorescence value of the control group × 100%.

[0089] Each group had 6 parallel experiments.

[0090] The results are shown in Table 3 and Figure 1 shown.

[0091] Table 3 Anti-glycation end products (AGEs) inhibition experiment

[0092]

[0093]

[0094] (*P<0.05 vs Example 2; **P<0.01 vs all groups)

[0095] in conclusion:

[0096] The AGEs inhibition rate of Example 5 (containing resveratrol + acerola cherry powder) was significantly higher than that of the aminoguanidine positive control (P<0.01) and the other examples and comparative example groups.

[0097] The inhibition rate of Comparative Example 5 (enzymolysis temperature increased to 50° C.) was lower than that of Example 2.

[0098] The inhibition rates of Comparative Example 1 (lacking sea cucumber peptide and soybean peptide), Comparative Example 3 (replacing yam polysaccharide with maltodextrin) and Comparative Example 4 (lacking pomegranate seed and grape seed extracts) were lower.

[0099] Test Example 2

[0100] DPPH free radical scavenging experiment

[0101] Objective: To evaluate the antioxidant capacity in vitro

[0102] Methods:

[0103] Sample treatment: Each group of freeze-dried powder was prepared into a 0.1 mg / mL aqueous solution.

[0104] Reaction system:

[0105] 0.1 mM DPPH ethanol solution 2 mL + sample solution 1 mL, avoid light reaction for 30 min, measure absorbance at 517 nm.

[0106] Clearance rate = (1 - sample absorbance / blank absorbance) x 100%.

[0107] Results are shown in Table 4 and Figure 2 .

[0108] Table 4 DPPH free radical scavenging experiment

[0109]

[0110]

[0111] (**P <0.01 vs Example 2)

[0112] The DPPH free radical scavenging rate of Example 5 was significantly higher than that of Example 2 (P <0.01), close to the VC positive control.

[0113] The clearance rates of Comparative Example 1 (missing sea cucumber peptide and soybean peptide) and Comparative Example 2 (missing tea polyphenol) were lower than that of Example 2.

[0114] Test Example 3

[0115] Determination of mouse skin hydroxyproline content

[0116] Objective: To verify the collagen regenerative capacity.

[0117] Methods:

[0118] Animal model: D-galactose-induced aging model (KM mice, 1 g / kg / d subcutaneous injection x 6 weeks).

[0119] Group gavage (n = 10):

[0120] Example 2 (200 mg / kg / d);

[0121] Example 5 (200 mg / kg / d);

[0122] Comparative Example 1 (200 mg / kg / d);

[0123] blank control group (normal saline);

[0124] Detection:

[0125] After 8 weeks, the back skin was collected and hydroxyproline was extracted by alkaline hydrolysis. The color was developed with p-dimethylaminobenzaldehyde and the colorimetry was performed at 558 nm.

[0126] The results are shown in Table 5 and Figure 3 .

[0127] Table 5 Determination of hydroxyproline content in mouse skin

[0128] Group Hydroxyproline (μg / mg) Improved compared to the blank group Blank control group 5.32±0.31 - Example 2 7.85±0.42* +47.6％ Example 5 9.26±0.38** +74.1％ Comparative Example 1 6.03±0.29 +13.3％

[0129] (*P<0.05, **P<0.01 vs blank group)

[0130] in conclusion:

[0131] The hydroxyproline content in the skin of mice orally administered with Example 2 and Example 5 was significantly higher than that in the blank control group (P<0.05 and P<0.01).

[0132] The hydroxyproline content and the increase in Example 5 are higher than those in Example 2.

[0133] The increase in hydroxyproline content in Comparative Example 1 (lacking sea cucumber peptide and soybean peptide) was significantly lower than that in Example 2 and Example 5.

[0134] Test Example 4

[0135] Proliferation inhibition of MCF-7 breast cancer cells

[0136] Objective: To evaluate the antitumor activity.

[0137] method:

[0138] Cell culture: MCF-7 cells (ATCC HTB-22), RPMI-1640 + 10% FBS.

[0139] Treatment group:

[0140] Example 2 (50 μg / mL);

[0141] Example 5 (50 μg / mL);

[0142] 5-Fluorouracil (5-FU, 10 μg / mL) was used as a positive control.

[0143] MTT assay:

[0144] 5×10 3 cells / well, add drugs and culture for 48h. Add MTT (5mg / mL) and incubate for 4h.

[0145] Formazan was dissolved in DMSO and the OD value was measured at 570 nm.

[0146] The results are shown in Table 6 and Figure 4 .

[0147] Table 6 MCF-7 breast cancer cell proliferation inhibition

[0148] Treatment group Inhibition rate (%) Example 2 38.7±3.2 Example 5 62.5±2.8** 5-FU 85.4±1.9

[0149] (**P<0.01 vs Example 2)

[0150] result:

[0151] The inhibition rate of Example 5 on the proliferation of MCF-7 breast cancer cells was significantly higher than that of Example 2 (P<0.01).

[0152] Both Example 2 and Example 5 showed proliferation inhibitory activity on MCF-7 cells, but the activity was lower than that of the positive control 5-FU.

[0153] Test Example 5

[0154] Evaluation of anti-wrinkle efficacy on mouse skin

[0155] Objective: To verify the improvement effect of the composition on ultraviolet photoaging

[0156] method:

[0157] Animal Models:

[0158] BALB / c mice (female, 18-20 g) were shaved on the back (2 x 3 cm area).

[0159] Ultraviolet irradiation: UVB lamp (wavelength 280-320nm), dose 180mJ / cm 2 , irradiation every other day, for 4 weeks.

[0160] Model verification: Scales and wrinkles appeared on the skin after irradiation (clinical score ≥ 3 points).

[0161] Group processing (n=8):

[0162] Model group: intragastric administration of normal saline;

[0163] Group 5 of Example 5: 200 mg / kg / d by oral gavage;

[0164] Positive control group: 0.05% tretinoin cream applied externally;

[0165] Normal control group: no irradiation + normal saline;

[0166] Detection indicators:

[0167] Skin wrinkle score: 0 points (smooth) to 4 points (deep wrinkles).

[0168] Histological analysis: The back skin was collected and HE staining was used to measure the epidermal thickness and Masson staining was used to calculate the collagen area ratio.

[0169] The results are shown in Table 7

[0170] Table 7: Evaluation of anti-wrinkle efficacy on mouse skin

[0171] Group Wrinkle score Epidermal thickness (μm) Collagen area ratio (%) Normal control group 1.0±0.1 15.3±1.2 82.5±3.1 Model Group 3.6±0.3 38.7±2.5 41.2±2.8 Example 5 Group 1.4±0.2** 22.1±1.8** 68.3±3.5** Retinoic acid positive group 1.1±0.3 19.5±1.6 73.8±2.9

[0172] (**P<0.01 vs model group)

[0173] in conclusion:

[0174] The skin wrinkle scores of mice in the ultraviolet irradiation model group were significantly increased, the epidermal thickness increased, and the collagen area ratio decreased.

[0175] The skin wrinkle scores, epidermal thickness and collagen area ratio of mice gavaged with Example 5 were significantly better than those of the model group (P<0.01), and were close to the levels of the retinoic acid positive control group and the normal control group.

[0176] The wrinkle score of the Example 5 group was reduced by 61% compared with the model group, the collagen area was increased by 66% compared with the model group, and the epidermal thickness was reduced compared with the model group.

[0177] Test Example 6

[0178] Acute toxicity test

[0179] Purpose: To assess safety

[0180] method:

[0181] KM mice (20±2 g) were orally administered with the solution of Example 5.

[0182] Maximum dosage: 5000 mg / kg (equivalent to 100 times the human dose).

[0183] Observation for 14 days:

[0184] Weight changes, food intake;

[0185] Organ coefficient (heart / liver / spleen / lung / kidney);

[0186] Complete blood test and biochemistry (ALT / AST / Cr);

[0187] result:

[0188] No animals died;

[0189] Weight gain was normal (Δ+4.2±0.3g);

[0190] Biochemical indices were within normal range (ALT: 35 ± 3 U / L vs. 32 ± 4 U / L in controls);

[0191] Conclusion: Under the experimental conditions, the maximum dosage of the composition of Example 5 for mice is greater than 5000 mg / kg body weight. According to the acute toxicity classification standard, the composition is practically non-toxic.

[0192] The test data is summarized as follows:

[0193] 1. Anti-glycation: Example 5 showed an AGE inhibition rate of 78.5%, significantly higher than the positive control aminoguanidine (58.3%) and the other comparative examples (lowest 24.6%). Increasing the enzymatic hydrolysis temperature to 50°C (Comparative Example 5) reduced the inhibition rate to 41.2%.

[0194] 2. Antioxidation: The DPPH scavenging rate of Example 5 was 91.3%, close to that of the VC positive control (94.6%), and significantly higher than that of Comparative Example 1 lacking peptides (48.2%) and Comparative Example 2 lacking tea polyphenols (63.7%).

[0195] 3. Collagen regeneration: Example 5 increased the hydroxyproline content in the skin of aged mice by 74.1% (9.26 μg / mg vs. 5.32 μg / mg in the blank group), which was significantly better than the comparative example 1 lacking the peptide (only increased by 13.3%).

[0196] 4. Anti-tumor: The inhibition rate of MCF-7 cell proliferation in Example 5 was 62.5%, which was significantly higher than that in Example 2 (38.7%) without adding resveratrol.

[0197] 5. Anti-wrinkle: Example 5 reduced the wrinkle score of UV-damaged mice by 61% (1.4 points vs. 3.6 points in the model group) and increased the collagen area ratio by 66% (68.3% vs. 41.2% in the model group), an effect close to that of the retinoic acid-positive group.

[0198] 6. Safety: In the acute toxicity test, no animals died at a dose of 5000 mg / kg, and body weight and biochemical indicators were normal, indicating that the drug is actually non-toxic.

[0199] The above are only a few preferred embodiments of the present invention, and their description is relatively specific and detailed, but it should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art may make various modifications and improvements without departing from the scope of the present invention, and such modifications and improvements are within the scope of protection of the present invention.

Claims

1. A yam polysaccharide composition, characterized in that: The composition includes, by mass: 30-80 parts of yam polysaccharide, 10-50 parts of stachyose, 5-25 parts of pomegranate seed extract, 5-20 parts of grape seed extract, 3-15 parts of blueberry extract, 2-12 parts of tea polyphenols, 1-10 parts of sea cucumber peptide and 1-10 parts of soybean peptide.

2. The composition according to claim 1, characterized in that It also includes 0.5 to 5 parts of resveratrol and 3 to 20 parts of acerola cherry powder.

3. The composition according to claim 1 or 2, characterized in that The ellagic acid content in the pomegranate seed extract is ≥40%, the proanthocyanidin content in the grape seed extract is ≥95%, and the anthocyanidin content in the blueberry extract is ≥25%.

4. The composition according to claim 1 or 2, characterized in that The average molecular weight of the sea cucumber peptide is 500-1500 Da, the average molecular weight of the soybean peptide is 300-1000 Da, and the average molecular weight of the yam polysaccharide is 10-50 kDa.

5. The composition according to claim 2, characterized in that The resveratrol purity is ≥98%, and the vitamin C content in the acerola cherry powder is ≥15%.

6. The composition according to claim 1 or 2, characterized in that The composition includes, by mass: 50 parts of yam polysaccharide, 30 parts of stachyose, 15 parts of pomegranate seed extract, 10 parts of grape seed extract, 8 parts of blueberry extract, 6 parts of tea polyphenols, 5 parts of sea cucumber peptide and 4 parts of soybean peptide.

7. The composition according to claim 6, characterized in that Also includes 2 parts resveratrol and 12 parts acerola cherry powder.

8. The method for preparing the composition according to any one of claims 1 to 7, wherein: The following steps are involved: (1) Mix yam polysaccharide and stachyose, add 5 to 10 times the mass of water, and stir to dissolve at 50 to 60°C; (2) adding pomegranate seed extract, grape seed extract, blueberry extract and tea polyphenols and homogenizing for 10 to 30 minutes; (3) adding sea cucumber peptide and soybean peptide, adjusting the pH to 6.0-7.0, and performing enzymatic hydrolysis at 35-40°C for 1-2 h; (4) When resveratrol is present, resveratrol is added after enzymatic hydrolysis; (5) When acerola cherry powder is present, add the acerola cherry powder and mix; (6) After vacuum concentration, spray drying is performed with an air inlet temperature of 100-115°C and an air outlet temperature of 65-85°C.

9. The preparation method according to claim 8, characterized in that The enzymatic hydrolysis in step (3) adopts compound protease, and the enzymatic hydrolysis conditions are: The added amount of the composite protease is 0.1-0.5 wt %, the enzymatic hydrolysis pH is 6.2-6.8, and the enzymatic hydrolysis time is 90-120 min.

10. Use of the composition according to any one of claims 1 to 7 in the preparation of health products, functional foods or skin repair preparations.

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