Portable solid-phase synthesis reaction device

Abstract

According to the portable solid-phase synthesis reaction device, in the portable solid-phase synthesis reaction device, a support module comprises a synchronous belt lead screw nut; the motor driving module is installed on one side of the upper end edge of the support module to serve as a power source component for adjusting the size of the composite column cavity, the motor driving module comprises a stepping motor and a synchronous wheel, the synchronous wheel is installed on an output shaft of the stepping motor, and the synchronous belt is arranged on the synchronous wheel and the synchronous belt lead screw nut in a sleeving mode; the lead screw lifting module is installed on the column top support to serve as an execution component for adjusting the size of the composite column cavity and comprises a transmission lead screw, a piston unit is installed at the lower end of the transmission lead screw, and the transmission lead screw is in threaded connection with a synchronous belt lead screw nut. The synthesis column body module is mounted in the middle between a column top support and a bottom plate of the support module to serve as a synthesis carrier to be filled and a solid-phase synthesis reaction vessel, and the synthesis column body module comprises a synthesis column.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of solid phase synthesis, and particularly relates to a portable solid phase synthesis reaction device. BACKGROUND

[0002] The principle of polypeptide solid phase synthesis is that an amino acid protected by an amino group is covalently crosslinked to a solid phase carrier, and the amino group is exposed by removing the amino protecting group using 20% piperidine N,N-dimethylformamide solution; then the next amino acid protected by an amino group, a condensing agent and an inhibitor are added, and the carboxyl group of the second amino acid is condensed with the amino group of the first amino acid to form an amide bond; in this way, a dipeptide with an amino protecting group is obtained on the solid phase carrier; the above reaction operation is repeated to extend the peptide chain from the C-terminal to the N-terminal until the required peptide sequence is obtained. The basic principle of nucleic acid solid phase synthesis is that the terminal nucleotide of the nucleic acid chain to be synthesized is first fixed on an insoluble high molecular solid phase carrier, and then other nucleotides are sequentially added from the terminal. The nucleic acid chain synthesized to the required length can be cut off from the solid phase carrier and the various protecting groups are removed, and then purified to obtain the final product. The synthesis column is a container for loading the solid phase carrier. In the prior art, the volume (which can also be briefly described as the height) of the synthesis column cavity needs to be manually adjusted by disassembling the bolts and locking mechanism, which is relatively cumbersome.

[0003] The information disclosed in the background section merely serves to enhance the understanding of the background of the present application, and therefore can contain information that is not prior art known to those of ordinary skill in the art. SUMMARY

[0004] In view of the deficiencies or defects of the prior art, a portable solid phase synthesis reaction device is provided, which can more conveniently adjust the volume of the column cavity through the electric mechanism according to the change of the volume of the synthesis column in the column cavity during the solid phase synthesis process, and solves the problem of the relatively cumbersome operation of manually disassembling and adjusting the volume of the synthesis column during the solid phase synthesis process.

[0005] The purpose of the present application is achieved by the following technical solutions.

[0006] A portable solid phase synthesis reaction device comprises, a support module comprising a synchronous belt screw nut; a motor drive module mounted on one side of the upper edge of the support module to serve as a power source component for adjusting the volume of the synthesis column cavity, the motor drive module comprising a stepper motor and a synchronous wheel, the synchronous wheel being mounted on the output shaft of the stepper motor, and the synchronous belt being sleeved on the synchronous wheel and the synchronous belt screw nut; The screw lifting module is installed on the support module to serve as an executive component for adjusting the volume of the synthetic column cavity. The screw lifting module includes a transmission screw, a piston unit is installed at the lower end of which, and the transmission screw is threadedly connected to the synchronous belt screw nut. The synthesis column module is installed on the support module to serve as a synthesis carrier filling and solid phase synthesis reaction vessel. The synthesis column module comprises a synthesis column which is slidably connected to the piston unit.

[0007] In the portable solid phase synthesis reaction device, the support module further comprises: bottom plate, A first support column and a second support column are erected on the bottom plate, The column top support is supported by the first support column and the second support column, and the column top support includes an end cover, a thrust needle roller bearing, a synchronous belt screw nut and a needle roller bearing.

[0008] In the portable solid-phase synthesis reaction device, the synchronous belt screw nut is clamped between the thrust needle bearing and the needle bearing and fixed to the column top support by the end cover. The upper surface of the column top support is provided with a groove and a through-hole, and the through-hole is connected to the hollow part of the first support column.

[0009] In the portable solid phase synthesis reaction device, the motor drive module further comprises: A motor connecting plate is installed on the column top support and is adapted to the groove. A stepper motor is installed on the motor connecting plate and is suspended on the outer side of the column top support.

[0010] In the portable solid-phase synthesis reaction device, the motor drive module further comprises a protective cover, which covers the motor connection plate, and the protective cover is provided with a first position sensor.

[0011] In the portable solid phase synthesis reaction device, the screw lifting module further comprises: The column top connecting piece is provided with a first optical coupling baffle, and the transmission screw is installed on the column top connecting piece. The first guide column is mounted on the column top connector, The second guide column is installed on the column top connector and a second optical coupling baffle is provided at the lower end of the second guide column. The first guide column and the second guide column are inserted through the through hole of the column top support and inserted into the hollow part of the first support column.

[0012] In the portable solid phase synthesis reaction device, the synthesis column module further comprises: A heating jacket, which surrounds the synthesis column to heat it, The column connecting plate is connected to the base plate to install the composite column module on the support module.

[0013] In the portable solid-phase synthesis reaction device, the control and cleaning module is installed on one side of the lower end edge of the support module to serve as a control unit for the volume of the synthesis column cavity. The control and cleaning module includes: Mounting bracket, which is fixed to the support module, The motor driver is mounted on the mounting bracket and connected to the stepping motor to control the lifting of the transmission screw to adjust the size of the synthesis cavity of the synthesis column. a second position sensor mounted on the mounting bracket, a sample bottle, which is fixed to the mounting bracket via a sample bottle clamp, and the sample bottle is used to contain a cleaning liquid; a peristaltic pump mounted on the mounting bracket for pumping the cleaning agent in the sample bottle; A control board is mounted on the mounting bracket and is connected to and controls the peristaltic pump and the motor driver.

[0014] In the portable solid-phase synthesis reaction device, the control and cleaning module further comprises a control panel protective cover, which covers the control panel, and the control panel comprises a PCB control panel.

[0015] In the portable solid-phase synthesis reaction device, the control board sends a signal to the motor driver, which controls the rotation of the stepper motor, driving the entire screw lift assembly to move upward until the second optical coupling baffle moves to the second position sensor, at which point the upward movement stops. The control board controls the rotation of the stepper motor, driving the entire screw lift assembly to move downward, causing the lower end of the piston unit to move to a set height position H. The screw lift is electrically adjusted to thereby control the volume of the synthesis column cavity.

[0016] In the portable solid-phase synthesis reaction device, the second optical coupling baffle and the second position sensor, as well as the first optical coupling baffle and the first position sensor, are respectively used as limiters for the upper and lower limit positions of the screw lifting assembly.

[0017] In the portable solid-phase synthesis reaction device, the piston unit includes two upper and lower piston parts located at the end of the screw rod, a cleaning chamber is formed between the two piston parts, the upper piston part is formed with two joints, both of which are connected to the cleaning chamber to form the inlet and outlet of the cleaning agent, the inlet pipeline of the peristaltic pump is connected to the sample bottle filled with the cleaning agent, the cleaning agent inlet is connected to the outlet pipeline of the peristaltic pump, and the cleaning agent outlet is connected to the sample bottle filled with the cleaning agent, thereby realizing a circulation loop of the cleaning agent between the sample bottle and the cleaning chamber of the piston unit.

[0018] Compared with the prior art, the present invention has the following beneficial effects: during the solid-phase synthesis process, there is a certain pressure in the column cavity. If manual adjustment is adopted by the experimenter, the manual adjustment takes an average of 2 minutes, and the manual adjustment is ±1.5mm, and the adjustment amplitude deviation is relatively large. The volume of the synthesis column cavity can be controlled relatively accurately through electronic control. The stepper motor + screw drive accurately controls the cavity volume. The electric adjustment takes ≤3 seconds, and the electric control cavity has a high error of ±0.1mm. The dual optical coupler limit system has dual safety protection and extends the service life. The piston has a built-in circulation cleaning chamber to avoid shutdown and disassembly for cleaning to interrupt the reaction. It has online self-cleaning and improves the continuity of synthesis. The modular assembly structure allows for quick disassembly and replacement of faulty parts.

[0019] The above description is only an overview of the technical solution of the present invention. In order to make the technical means of the present invention clearer and easier to understand, so that those skilled in the art can implement it according to the contents of the description, and in order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are illustrated below. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Various other advantages and benefits of the present invention will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiments below. The accompanying drawings are intended only to illustrate preferred embodiments and are not to be construed as limiting the present invention. It should be understood that the drawings described below are merely examples of the present invention, and that those skilled in the art will be able to derive other drawings from these drawings without inventive effort. Throughout the drawings, identical reference numerals are used to denote identical components.

[0021] In the attached figure: Figure 1 A schematic diagram showing the overall assembly of a convenient solid-phase synthesis reaction device of the present invention is shown; Figure 2 A schematic structural diagram of a support module according to an embodiment of the present invention is shown; Figure 3 A schematic diagram showing the structure of a motor drive module according to an embodiment of the present invention is shown; Figure 4 A schematic diagram showing the structure of a screw lifting module of a portable solid-phase synthesis reaction device of the present invention is shown; Figure 5 A schematic diagram showing the main structure of a convenient solid-phase synthesis reaction device of the present invention is shown; Figure 6 A schematic diagram showing the structure of a control and cleaning module of a portable solid-phase synthesis reaction device of the present invention is shown; Figure 7A schematic diagram showing the cleaning of a portable solid-phase synthesis reaction device according to the present application is shown. Figure 8 A schematic diagram showing the flow path of a solvent in a portable solid-phase synthesis reaction device according to the present application is shown.

[0022] The present application will be further explained in conjunction with the accompanying drawings and examples. DETAILED DESCRIPTION

[0023] Specific embodiments of the present application will be described in greater detail below with reference to the accompanying drawings. While the present application is shown in these drawings, it is understood that the present application can be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that the present application can be more thoroughly understood and so that the full scope of the present application can be conveyed to those skilled in the art.

[0024] It should be noted that certain terms are used throughout the specification and claims which have particular meanings. Those skilled in the art will understand that the same component can be referred to by different names. The present specification and claims do not serve as a basis to distinguish components by the difference in names. Rather, the present specification and claims serve as a basis to distinguish components by the difference in functions. As mentioned throughout the specification and claims, "comprise" or "include" is an open term, and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment for implementing the present application, and is intended to serve as a purpose of the general principles of the specification, and not to limit the scope of the present application. The scope of protection of the present application is defined by the appended claims.

[0025] In order to facilitate the understanding of the embodiments of the present application, the following will be further explained and described with reference to the accompanying drawings in conjunction with several specific embodiments, and each of the accompanying drawings does not constitute a limitation on the embodiments of the present application.

[0026] In order to better understand, as shown in Figures 1 to 8 A portable solid-phase synthesis reaction device includes, a support module 100 including a synchronous belt screw nut 107; in the preferred embodiment of the portable solid-phase synthesis reaction device, the support module 100 further includes, a bottom plate 101, a first support column 102 and a second support column 105 which are erected on the bottom plate 101, The column top support 103 is supported by the first support column 102 and the second support column 105. The column top support 103 includes an end cap 104, a thrust needle roller bearing 108, a synchronous belt screw nut 107, and a needle roller bearing 106. The synchronous belt screw nut 107 is sandwiched between the thrust needle roller bearing 108 and the needle roller bearing 106 and fixed to the column top support 103 by the end cap 104. The upper surface of the column top support 103 is provided with a groove and a through-hole, which is connected to the hollow portion of the first support column 102. The motor drive module 300 is installed on the upper edge of the support module 100 to serve as a power source component for adjusting the volume of the synthetic column cavity. The motor drive module 300 includes a stepper motor 304 and a synchronous pulley 305. The synchronous pulley 305 is installed on the output shaft of the stepper motor 304. The synchronous belt 302 is sleeved on the synchronous pulley 305 and the synchronous belt screw nut 107. The screw lifting module 400 is mounted on the column top support 103 to serve as an actuator for adjusting the volume of the synthetic column cavity. The screw lifting module 400 includes a transmission screw 406, a piston unit 401 is mounted at its lower end, and the transmission screw 406 is threadedly connected to the synchronous belt screw nut 107; The synthesis column module 500 is installed in the middle between the column top support 103 and the bottom plate 101 of the support module 100 to serve as a synthesis carrier filling and solid phase synthesis reaction vessel. The synthesis column module 500 includes a synthesis column 503 .

[0027] In a preferred embodiment of the portable solid phase synthesis reaction device, the motor drive module 300 further includes: The motor connecting plate 301 is mounted on the column top support 103 and fits into the groove. The stepping motor 304 is mounted on the motor connecting plate 301 and hangs on the outside of the column top support 103. The protective cover 306 covers the motor connecting plate 301 , and the protective cover 306 is provided with a first position sensor 303 .

[0028] In a preferred embodiment of the portable solid-phase synthesis reaction device, the screw lifting module 400 further includes: The column top connecting piece 403 is provided with a first optical coupling baffle 409, and the transmission screw 406 is installed on the column top connecting piece 403. The first guide column 402 is mounted on the column top connector 403. The second guide column 405 is installed on the column top connector 403 and a second optical coupling baffle 404 is provided at the lower end of the second guide column 405. The first guide column 402 and the second guide column 405 are inserted through the through hole of the column top support 103 and inserted into the hollow part of the first support column 102.

[0029] The preferred embodiment of the portable solid-phase synthesis reaction device, the synthesis column module 500 further comprises, a heating jacket 502 surrounding the synthesis column 503 to heat, a column connecting plate 501 connected to the base plate 101 to mount the synthesis column module 500 to the support module 100.

[0030] The preferred embodiment of the portable solid-phase synthesis reaction device, the piston unit 401 comprises two piston parts on the end of the lead screw, forming a cleaning cavity between the two piston parts, the upper piston part forms two joints, both joints are in communication with the cleaning cavity as the cleaning agent inlet and outlet, the inlet pipeline of the peristaltic pump is connected to the sample bottle containing the cleaning agent, the cleaning agent inlet is connected to the outlet pipeline of the peristaltic pump, the cleaning agent outlet is connected to the sample bottle containing the cleaning agent, realizing the circulation loop of the cleaning agent between the sample bottle and the cleaning cavity of the piston unit. The preferred embodiment of the portable solid-phase synthesis reaction device, the portable solid-phase synthesis reaction device further comprises a control and cleaning module 600 mounted on one side of the lower edge of the support module 100 as a control unit of the synthesis column cavity volume, the control and cleaning module 600 comprises, a mounting bracket 601 fixedly connected to the support module 100, a motor driver 602 mounted on the mounting bracket 601 and connected to the stepper motor 304 to control the lifting of the transmission lead screw 406 to adjust the size of the synthesis cavity of the synthesis column 503, a second position sensor 603 mounted on the mounting bracket 601, a sample bottle 605 fixed to the mounting bracket 601 through a sample bottle clamp 604, the sample bottle 605 is used to contain cleaning liquid, a peristaltic pump 606 mounted on the mounting bracket 601 for pumping the cleaning agent in the sample bottle 605, a control board 607 mounted on the mounting bracket 601 and connected to and controlling the peristaltic pump 606 and the motor driver 602.

[0031] In a preferred embodiment of the portable solid-phase synthesis reaction device, the control and cleaning module 600 further includes a control panel protective cover 608, which covers the control panel 607, which includes a PCB control board. In a preferred embodiment of the portable solid-phase synthesis reaction device, the control panel 607 sends a signal to the motor driver 602, which controls the rotation of the stepper motor 304, driving the screw lift module 400 to move upward as a whole until the second optical coupling baffle 404 moves to the second position sensor 603, at which point the upward movement stops. The control panel 607 then controls the rotation of the stepper motor 304, driving the screw lift assembly 400 to move downward as a whole, causing the lower end of the piston unit 401 to move to a set height position H. The screw lift is then electrically adjusted to control the volume of the synthesis column cavity.

[0032] In the preferred embodiment of the portable solid-phase synthesis reaction device, the second optical coupling baffle 404 and the second position sensor 603 , as well as the first optical coupling baffle 409 and the first position sensor 303 are respectively used as limiters for the upper and lower limit positions of the screw lifting assembly 400 .

[0033] In one embodiment, a convenient solid phase synthesis reaction device comprises: The support module 100 comprises: Base plate 101, The first support column 102 and the second support column 105 are erected on the bottom plate 101. The column top support 103 is supported by the first support column 102 and the second support column 105. The column top support 103 includes an end cover 104, a thrust needle roller bearing 108, a synchronous belt screw nut 107, and a needle roller bearing 106. The synchronous belt screw nut 107 is clamped between the thrust needle roller bearing 108 and the needle roller bearing 106 and fixed to the column top support 103 by the end cover 104. The upper surface of the column top support 103 is provided with a groove and a through-hole, which is connected to the hollow portion of the first support column 102. The motor drive module 300 is installed on one side of the upper edge of the support module 100 to serve as a power source component for adjusting the volume of the synthetic column cavity. The motor drive module 300 includes: The motor connecting plate 301 is mounted on the column top support 103 and fits into the groove. The stepper motor 304 is mounted on the motor connecting plate 301 and is suspended on the outside of the column top support 103. The synchronous wheel 305 is mounted on the output shaft of the stepping motor 304, and the synchronous belt 302 is sleeved on the synchronous wheel 305 and the synchronous belt screw nut 107. A protective cover 306 covers the motor connecting plate 301 and is provided with a first position sensor 303; The screw lifting module 400 is installed on the column top support 103 to serve as an executive component for adjusting the volume of the synthetic column cavity. The screw lifting module 400 includes: The column top connecting member 403 is provided with a first optical coupling baffle 409. The transmission screw 406 is installed on the column top connector 403 and the piston unit 401 is installed at the lower end of the transmission screw 406. The transmission screw 406 is threadedly connected to the synchronous belt screw nut 107. The first guide column 402 is mounted on the column top connector 403. The second guide column 405 is mounted on the column top connector 403 and a second optical coupling baffle 404 is provided at the lower end of the second guide column 405. The first guide column 402 and the second guide column 405 are inserted through the through hole of the column top support 103 and inserted into the hollow part of the first support column 102. The synthesis column module 500 is installed in the middle between the column top support 103 and the bottom plate 101 of the support module 100 to serve as a synthesis carrier filling and solid phase synthesis reaction vessel. The synthesis column module 500 includes: Synthetic Column 503, A heating jacket 502, which surrounds the synthesis column 503 to heat, a column connecting plate 501 connected to the base plate 101 to mount the composite column module 500 on the support module 100; The control and cleaning module 600 is installed on one side of the lower edge of the support module 100 to serve as a control unit for synthesizing the column cavity volume. The control and cleaning module 600 includes: The mounting bracket 601 is fixedly connected to the support module 100. The motor driver 602 is mounted on the mounting bracket 601 and connected to the stepping motor 304 to control the lifting and lowering of the transmission screw 406 to adjust the size of the synthesis cavity of the synthesis column 503. The second position sensor 603 is mounted on the mounting bracket 601. The sample bottle 605 is fixed to the mounting bracket 601 via the sample bottle clamp 604. The sample bottle 605 is used to contain the cleaning liquid. The peristaltic pump 606 is mounted on the mounting bracket 601 and is used to pump the cleaning agent in the sample bottle 605. One end of the peristaltic pump 606 is connected to the sample bottle 605, and the other end is connected to the cleaning liquid cavity of the piston. The control board 607 is mounted on the mounting bracket 601 and is connected to and controls the peristaltic pump 606 and the motor driver 602. The control panel protective cover 608 covers the control panel 607 .

[0034] In one embodiment, the portable solid phase synthesis reaction device includes a support module, a motor drive module, a screw lifting module, a synthesis body module, and a control and cleaning module. Figure 1 .

[0035] The support module is shown in FIG. Figure 2 As the basic component of the entire device, it is used to position or install other related components; the support module 100 includes a base plate 101, a plurality of, for example, three, first support columns 102 and a second support column 105 standing on the base plate 101, and a column top support 103 supported by the support columns. The support columns can be hollow so that the first guide column 402 and the second guide column 405 of the screw lifting module can be inserted therein. The first support column 102 and the second support column 105 can also be provided with a longitudinal groove so that the second optical coupling baffle 404 of the second guide column 405 can extend out of the support column.

[0036] The column top support 103 of the support module 100 includes an end cap 104, a thrust needle roller bearing 108, a synchronous belt screw nut 107, and a needle roller bearing 106. The synchronous belt screw nut 107 is sandwiched between the thrust needle roller bearing 108 and the needle roller bearing 106 and secured to the column top support 103 by the end cap 104. The top surface of the column top support 103 includes a groove for mating with the motor connecting plate 301 of the motor drive module 300. The top surface of the column top support 103 also has a perforation that connects to the hollow portion of the first support column 102, allowing the first guide column 402 and the second guide column 405 of the screw lifting module to be inserted.

[0037] The motor drive module Figure 3 The power source component for adjusting the volume of the synthetic column cavity is installed on one side of the upper edge of the support module. It includes a motor connecting plate 301 and a stepper motor 304 mounted on the motor connecting plate 301. A synchronous pulley 305 is mounted on the output shaft of the stepper motor 304, and a synchronous belt 302 is sleeved over the synchronous pulley 305 and the synchronous belt screw nut 107. The motor connecting plate 301 is mounted on the column top support 103, and the stepper motor 304 is suspended outside the column top support 103. A protective cover 306 is provided on the motor connecting plate 301, and a first position sensor 303 is mounted on the protective cover 306.

[0038] The screw lifting module Figure 4As an executive component for adjusting the volume of the synthetic column cavity, it is installed above the support module, and the volume of the synthetic column cavity is adjusted by rising and falling the piston unit installed thereon; the screw lifting module 400 includes a column top connector 403, a transmission screw 406, a first guide column 402, and a second guide column 405. The transmission screw 406, the first guide column 402, and the second guide column 405 are all installed on the column top connector 403. A first optical coupling baffle 409 is also provided on the column top connector 403, and a second optical coupling baffle 404 is provided at the lower end of the second guide column 405. A piston unit 401 is installed at the lower end of the transmission screw 406. When the screw lifting module 400 is installed on the column top support 103 of the support module 100, the transmission screw 406 is threadedly connected to the synchronous belt screw nut 107, and the first guide column 402 and the second guide column 405 of the screw lifting module are inserted through the through-holes on the upper surface of the column top support 103 and inserted into the hollow part of the first support column 102.

[0039] The synthetic column module is shown in FIG. Figure 5 As a synthetic carrier filling and solid-phase synthesis reaction vessel, it is installed in the middle of the support module, that is, between the column top support 103 and the base plate 101. The synthetic column module 500 includes a synthetic column 503, a heating jacket 502 surrounding the synthetic column 503 and a column connecting plate 501 fixed under the synthetic column 503. The synthetic column module 500 is installed on the support module by connecting the column connecting plate 501 of the synthetic column module 500 with the base plate 101 of the support module 100.

[0040] The control and cleaning module Figure 6 As the control unit of the synthetic column cavity volume, it actually controls the lifting and lowering of the screw by controlling the operation of the motor, and thus indirectly controls the size of the synthetic column synthetic cavity, as well as the synthetic column cavity cleaning and lubrication unit, which is installed on the lower edge of the support module and below the motor drive module.

[0041] In the device, the rotation of the stepper motor 304 is first controlled by the program in the control board 607, driving the screw lifting assembly 400 to move upward as a whole until the second optical coupling baffle 404 moves to the second position sensor 603 and stops moving upward; after the experimenter adds filler to the column 503 in the synthetic column assembly 500, it is installed on the bottom plate 101 of the support module 100, and then the screw lifting module 400 is controlled by the program to move downward as a whole, so that the lower end of the piston unit 401 moves to the set height position H (when adding filler, the volume V of the synthetic column cavity is determined according to its expansion coefficient. Since the inner diameter r of the synthetic column is known, the value of H can be roughly calculated). Subsequently, according to the actual expansion of the filler added to the synthetic column 503, the screw lifting is adjusted by program electronic control to control the volume of the synthetic column cavity, that is, the height H of the piston unit 401. During the solid-phase synthesis process of this device, there is a certain pressure in the column cavity. If the experimenter manually adjusts it, the adjustment range will have a large deviation. Through electronic control, the actual volume of the synthesis column cavity can be relatively accurately controlled to the height H.

[0042] Controlling the rotation of the stepper motor 304 to drive the screw lifting assembly 400 to move downward as a whole until the first optical coupling baffle 409 moves to the first position sensor 303 and stops moving downward; The second optical coupler baffle 404 and the second position sensor 603, as well as the first optical coupler baffle 409 and the first position sensor 303, respectively serve as limiters for the upper and lower limits of the screw lift assembly 400. The piston comprises upper and lower piston portions located at the ends of the screw, forming a cleaning chamber between the two piston portions. The upper piston portion has two connectors, both of which communicate with the cleaning chamber to serve as inlets and outlets for the cleaning agent. The inlet line of the peristaltic pump is connected to a sample bottle containing cleaning agent, the cleaning agent inlet is connected to the outlet line of the peristaltic pump, and the cleaning agent outlet is connected to a sample bottle containing cleaning agent. During the upward and downward movement of the piston unit 401, the cleaning agent in the cleaning chamber can clean the inner wall of the synthetic column cavity.

[0043] like Figure 7 As shown, the PCB main control board 607 directly controls the start and stop of the peristaltic pump 606. Position and speed requirements are not involved, so the start and stop can be controlled directly using an on-off method. The PCB main control board 607 sends a signal to the stepper motor driver 602, which controls the speed and accuracy of the stepper motor 304 (this involves the vertical position accuracy of the piston unit 401). The sample bottle 605 contains a cleaning agent. During the upward and downward movement of the transmission screw 406, the cleaning agent in the sample bottle is circulated through the pipeline according to the illustrated scheme through the operation of the peristaltic pump. In actual operation, normal operation can also be achieved even if the flow path flows in the opposite direction.

[0044] like Figure 8As shown, the column top connector 403 is provided with a port A, the bottom end of the piston is provided with a port B, the screw lifting module has an internal flow channel connecting the A port and the B port, and the bottom of the column connecting plate 501 is provided with a port C. Regarding the flow path of the reaction solvent, in the actual laboratory operation process, it can be from A-B-C or C-B-A, and the section between the B port and the C port can be abstractly understood as the space where the synthesis column actually performs solid-phase synthesis.

[0045] In one embodiment, the stepper motor 304 is controlled to rotate by the program of the control board 607, driving the screw lifting module 400 to move upward as a whole until the second optical coupling baffle 404 at the lower end of the second guide column 405 triggers the second position sensor 603 to stop the movement. The experimenter adds solid-phase filler into the synthetic column 503 of the synthetic column module 500, and then fixes the synthetic column module to the bottom plate 101 of the support module 100 through the column connecting plate 501, and controls the screw lifting module 400 to move downward as a whole: the transmission screw 406 drives the piston unit 401 downward under the drive of the synchronous belt screw nut 107. The target height is calculated according to the filler expansion coefficient: H=V / πr 2 , where V is the desired chamber volume and r is the inner diameter of the synthesis column 503. The lower end of the piston unit 401 moves to the set height H, forming a precise synthesis reaction chamber. Between synthesis reactions, a cleaning cycle is initiated: peristaltic pump 606 draws detergent from the detergent supply bottle. The detergent enters the cleaning chamber inlet of piston unit 401 via the blue pipeline. The detergent flows through the cleaning chamber formed by the dual-piston structure, flushing the inner wall of the synthesis column 503. Waste liquid returns to the detergent recovery bottle via the red pipeline, forming a closed-loop flow path. Lower limit protection: When the first optical coupler baffle 409 triggers the first position sensor 303, the downward movement of the screw lift module 400 is stopped. Upper limit protection: When the second optical coupler baffle 404 triggers the second position sensor 603, the upward movement of the screw lift module 400 is stopped.

[0046] In one embodiment, two hollow first support columns 102 and one second support column 105 are vertically fixed to the base plate 101. The column top support 103 compresses the thrust needle bearing 108 and the needle bearing 106 through the end cover 104, and fixes the synchronous belt screw nut 107 therebetween. A longitudinal groove is provided on the side wall of the first support column 102 for inserting the second guide column 405, so that the second optical coupling baffle 404 can extend out of the first support column 102. The stepper motor 304 is suspended and fixed to the side of the column top support 103 through the motor connecting plate 301. The synchronous wheel 305, the synchronous belt 302 and the synchronous belt screw nut 107 form a belt drive. The first position sensor 303 is embedded in the top of the protective cover 306. The lower end of the transmission screw 406 is connected to the piston unit 401, and the upper end passes through the first guide column 402 and the second guide column 405 of the column top connecting piece 403 and is fixed parallel to the column top connecting piece 403. The first optical coupling baffle 409 is horizontally fixed to the side of the column top connecting piece 403. The second position sensor 603 is installed in the longitudinal groove position of the support column 102, and the peristaltic pump 606 is connected through a pipeline.

[0047] The present invention achieves micron-level precision through pulse control of the stepper motor, while the synchronous belt drive can avoid the return error caused by gear clearance. Traditional mechanical limit switches are prone to wear, and the non-contact detection of optical couplers ensures both precision and life. The height of the synthesis column cavity can be controlled relatively accurately through electronic control. Each pulse of the stepper motor corresponds to a lead screw displacement of 0.002mm, achieving a cavity height control accuracy of ±0.1mm. The inner wall of the cavity is cleaned during the movement of the piston unit. The cleaning chamber formed by the upper and lower pistons is essentially a mobile sealed cabin, which completes the cleaning while adjusting the height. Compared with traditional shutdown and disassembly cleaning, this dynamic sealing structure can maintain the stability of the reaction chamber pressure, which is crucial for polypeptide synthesis. The closed-loop flow path design of the peristaltic pump also reduces the consumption of cleaning agents. The cleaning agent forms turbulence at a pressure of 0.2MPa, with a removal rate of >92%, avoiding the reaction temperature fluctuation of ±5℃→±0.3℃ caused by traditional disassembly and cleaning, and reducing the risk of filler exposure and oxidation failure by 70%.

[0048] The basic principles of the present application have been described above in conjunction with specific embodiments. However, it should be noted that the advantages, strengths, and effects mentioned in this application are merely illustrative and not restrictive, and it should not be assumed that these advantages, strengths, and effects are required of each embodiment of this application. In addition, the specific details disclosed above are merely illustrative and facilitating understanding, and are not restrictive. The above details do not limit this application to necessarily being implemented using the above specific details.

[0049] The above description has been provided for the purpose of illustration and description. Furthermore, this description is not intended to limit the embodiments of the present application to the forms disclosed herein. Although a number of example aspects and embodiments have been discussed above, those skilled in the art will recognize certain variations, modifications, alterations, additions, and sub-combinations thereof.

Claims

1. A portable solid phase synthesis reaction device, characterized in that: These include, A support module (100) comprising a timing belt screw nut (107); A motor drive module (300) is mounted on one side of the upper end edge of the support module (100) to serve as a power source component for adjusting the volume of the synthetic column cavity. The motor drive module (300) includes a stepping motor (304) and a synchronous wheel (305). The synchronous wheel (305) is mounted on the output shaft of the stepping motor (304). The synchronous belt (302) is sleeved on the synchronous wheel (305) and the synchronous belt screw nut (107). The screw lifting module (400) is installed on the support module (100) to serve as an executive component for adjusting the volume of the synthetic column cavity. The screw lifting module (400) includes a transmission screw (406), a piston unit (401) is installed at the lower end of which, the transmission screw (406) is threadedly connected to the synchronous belt screw nut (107). The synthesis column module (500) is installed on the support module (100) to serve as a synthesis carrier filling and solid phase synthesis reaction vessel. The synthesis column module (500) includes a synthesis column (503) slidably connected to the piston unit (401).

2. The portable solid phase synthesis reaction device according to claim 1, characterized in that: The support module (100) further comprises, Bottom plate (101), A first support column (102) and a second support column (105) are erected on the bottom plate (101), The column top support (103) is supported by the first support column (102) and the second support column (105), and the column top support (103) includes an end cover (104), a thrust needle roller bearing (108), a synchronous belt screw nut (107) and a needle roller bearing (106).

3. The portable solid phase synthesis reaction device according to claim 2, characterized in that: The synchronous belt screw nut (107) is clamped between the thrust needle bearing (108) and the needle bearing (106) and fixed to the column top support (103) by the end cover (104). The upper surface of the column top support (103) is provided with a groove and a through hole, and the through hole is connected to the hollow part of the first support column (102).

4. The portable solid phase synthesis reaction device according to claim 3, characterized in that: The motor drive module (300) further includes, A motor connecting plate (301) is mounted on the column top support (103) and adapted to the groove; a stepping motor (304) is mounted on the motor connecting plate (301) and suspended on the outside of the column top support (103).

5. The portable solid phase synthesis reaction device according to claim 4, characterized in that: The motor drive module (300) further comprises a protective cover (306) which covers the motor connection plate (301); the protective cover (306) is provided with a first position sensor (303).

6. The portable solid phase synthesis reaction device according to claim 5, characterized in that: The screw lifting module (400) further includes, The column top connecting piece (403) is provided with a first optical coupling baffle (409), and the transmission screw (406) is installed on the column top connecting piece (403). A first guide column (402) is mounted on a column top connector (403), The second guide column (405) is mounted on the column top connector (403) and a second optical coupling baffle (404) is provided at the lower end of the second guide column (405). The first guide column (402) and the second guide column (405) are inserted through the through hole of the column top support (103) and inserted into the hollow portion of the first support column (102).

7. The portable solid phase synthesis reaction device according to claim 6, characterized in that: The composite column module (500) further comprises, A heating jacket (502) that surrounds the synthesis column (503) to heat, The column connecting plate (501) is connected to the base plate (101) to install the composite column module (500) on the support module (100).

8. The portable solid phase synthesis reaction device according to claim 6, characterized in that: The control and cleaning module (600) is installed on one side of the lower end edge of the support module (100) to serve as a control unit for synthesizing the column cavity volume. The control and cleaning module (600) includes: The mounting bracket (601) is fixedly connected to the support module (100), The motor driver (602) is mounted on the mounting bracket (601) and connected to the stepping motor (304) to control the lifting and lowering of the transmission screw (406) to adjust the size of the synthesis cavity of the synthesis column (503). A second position sensor (603) is mounted on the mounting bracket (601), A sample bottle (605) is fixed to the mounting bracket (601) via a sample bottle clamp (604), and the sample bottle (605) is used to contain a cleaning solution. a peristaltic pump (606), which is mounted on the mounting bracket (601) and is used to pump the cleaning agent in the sample bottle (605), A control board (607) is mounted on the mounting bracket (601) and is connected to and controls the peristaltic pump (606) and the motor driver (602).

9. The portable solid phase synthesis reaction device according to claim 8, characterized in that: The control and cleaning module (600) further comprises a control panel protective cover (608), wherein the control panel protective cover (608) covers the control panel (607), and the control panel (607) comprises a PCB control panel.

10. The portable solid phase synthesis reaction device according to claim 8, characterized in that: The control board (607) sends a signal to the motor driver (602), and the motor driver (602) controls the rotation of the stepper motor (304), driving the screw rod lifting assembly (400) to move upward as a whole until the second optical coupling baffle (404) moves to the second position sensor (603), and then stops moving upward. The control board (607) controls the rotation of the stepper motor (304), driving the screw rod lifting assembly (400) to move downward as a whole, so that the lower end of the piston unit (401) moves to a set height position H, and the screw rod lifting is adjusted by electric control, thereby controlling the volume of the synthetic column cavity.

11. The portable solid phase synthesis reaction device according to claim 8, characterized in that: The second optical coupling baffle (404) and the second position sensor (603), as well as the first optical coupling baffle (409) and the first position sensor (303), are respectively used as limiters for the upper and lower limit positions of the screw lifting assembly (400).

12. The portable solid phase synthesis reaction device according to claim 1, characterized in that: The piston unit (401) includes two upper and lower piston parts located at the end of the screw rod, a cleaning chamber is formed between the two piston parts, the upper piston part is formed with two joints, both of which are connected to the cleaning chamber to form the inlet and outlet of the cleaning agent, the inlet pipe of the peristaltic pump is connected to the sample bottle containing the cleaning agent, the cleaning agent inlet is connected to the outlet pipe of the peristaltic pump, and the cleaning agent outlet is connected to the sample bottle containing the cleaning agent, thereby realizing a circulation loop of the cleaning agent between the sample bottle and the cleaning chamber of the piston unit (401).

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