Method for recycling waste residues generated in production process of fruit juice and fruit pulp
By using pectin enzymatic hydrolysis and composite fermentation bacteria to treat waste residues from juice and pulp production, the problem of low waste residue treatment efficiency is solved, high-efficiency organic fertilizer is prepared, resource waste is reduced, and crop growth is promoted.
Patent Information
- Application Number
- CN202511026717.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-24
- Publication Date
- 2025-10-17
AI Technical Summary
The waste residue generated during the production of juice and pulp has low treatment efficiency, poor direct composting and fermentation effect, is prone to produce odor and waste resources, and is difficult to efficiently convert into organic fertilizer.
Pectinase and laccase were used for enzymatic pretreatment, attapulgite and cocamidopropyl betaine were added as regulators, and fermentation was carried out in combination with composite fermentation bacteria such as Aspergillus niger, Lactobacillus plantarum, Nitrogen-fixing Bacterium chlorosphaeroides and Bacillus stearothermophilus. The treatment steps were optimized to improve the composting rate and decomposition efficiency.
It achieves efficient fermentation and composting of waste residues, prepares excellent solid organic fertilizer matrix and liquid enzyme fertilizer, reduces resource waste, improves the fertility and utilization rate of organic fertilizer, reduces environmental pollution, promotes crop growth and prevents diseases.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of fruit waste residue recycling, and particularly relates to a recycling method of waste residue generated in the production process of fruit juice and fruit pulp. BACKGROUND
[0002] Sugarcane and tangerines are widely planted in Guangxi. The fruit juice and fruit pulp obtained by physical methods such as pressing, centrifugation and extraction from fresh sugarcane and tangerines are delicious and fresh, have high nutritional value, and are loved by people. Sugarcane juice contains sucrose, glucose and fructose, can quickly replenish energy and relieve fatigue, and also has rich mineral substances, vitamins and other nutrients, has the effects of generating saliva to relieve thirst, clearing heat and moistening the lungs. Tangerine juice is rich in organic acids, vitamin C and other nutrients, has the effects of beautifying and nourishing the skin, and enhancing immunity. In the fruit juice and fruit pulp processing process, a large amount of by-products such as fiber residue and fruit residue are generated. These waste residues contain rich cellulose, hemicellulose, pectin, sugar and mineral substances, and have high added value. At present, the traditional treatment methods are mostly landfill or incineration, which not only causes resource waste, but also causes environmental pollution problems. With the global emphasis on sustainable development and circular economy, developing efficient waste residue recycling technology has become an urgent need of the industry.
[0003] At present, the recycling ways of fruit residue mainly include feed, energy (such as biogas fermentation) and fertilizer. The feed processing is limited by single nutritional composition and potential pesticide residue risk; the energy technology can realize part of resource recovery, but has high investment cost and low energy conversion efficiency. Compared with the above, the preparation of organic fertilizer has the advantages of low technical threshold, high added value and stable market demand. However, there are still the following problems in the preparation process of organic fertilizer: the degradation efficiency of direct composting of fiber residue and fruit residue mixture is low, and the fermentation time is long; the high sugar content in fruit residue is easy to ferment and produce odor, and the lignified structure of fiber residue hinders the decomposition of microorganisms, so the direct composting fermentation effect is poor and the composting is slow. SUMMARY
[0004] In view of the above problems, the present application provides a recycling method of waste residue generated in the production process of fruit juice and fruit pulp, which can efficiently ferment and decompose the waste residue generated in the production process of fruit juice and fruit pulp to form excellent organic fertilizer. By optimizing the processing steps, the fermentation efficiency is improved, and the fertility and availability of the organic fertilizer are improved. The specific technical scheme is as follows: A recycling method of waste residue generated in the production process of fruit juice and fruit pulp, comprising the following steps: (1) crushing the first pressed tangerine peel residue and tangerine fruit residue, and then performing secondary pressing and filtration to obtain tangerine waste residue and tangerine waste liquid; (2) chopping the bagasse into short sections less than 2 cm, mixing the chopped bagasse with the Wogan waste residue obtained in step (1) in a weight ratio of 1:2 to 4 to obtain a mixed residue, adding a regulator and stirring and mixing, wherein the regulator consists of attapulgite and cocamidopropyl betaine to obtain a mixed material; (3) adding pectin lyase and laccase to the mixed material obtained in step (2) and mixing them, and then performing enzymatic pretreatment at a temperature of 25-50° C. for 4-6 hours to obtain a pretreated mixed material; (4) adding a composite fermentation agent to the mixed material obtained by the pretreatment in step (3), wherein the composite fermentation agent includes Aspergillus niger, Lactobacillus plantarum, Nitrogen-fixing Bacterium chlorophyllus, and Geobacillus stearothermophilus, and composting and fermenting the mixed material for 5 to 7 days, turning the compost, and continuing the composting and fermentation for 10 to 15 days to obtain a solid organic fertilizer matrix; (5) Adding Aspergillus oryzae and brewer's yeast to the citrus waste liquid obtained in step (1) and fermenting to obtain enzyme liquid fertilizer.
[0005] In the method of the present invention, pectinase and laccase are first used to perform enzymatic hydrolysis pretreatment on a mixture containing sugarcane bagasse and pomace, which can degrade pectin, break the lignin structure, reduce the coating of pectin on fiber residue, and interrupt the close combination of cellulose, hemicellulose and lignin, which is conducive to the effective degradation of subsequent microorganisms. Adding a regulator to the mixture can increase the composting rate of enzymatic hydrolysis and fermentation treatment, and improve the composting degree of organic fertilizer; a composite fermentation agent is used to ferment and mature the mixture after enzymatic hydrolysis pretreatment, efficiently decompose cellulose in sugarcane bagasse / pomace, ferment sugars to generate organic acids such as lactic acid to inhibit harmful bacteria and reduce odor generation; reduce the loss of nutrients, increase active substances such as amino acids and vitamins, and prepare an excellent solid organic fertilizer matrix; at the same time, waste liquid is fermented to form enzyme liquid fertilizer, further utilizing waste from the fruit juice and pulp production process, and by optimizing steps and parameters, waste residues generated in the fruit juice and pulp production process can be efficiently fermented and recycled, reducing resource waste.
[0006] Preferably, in the above-mentioned method for recycling waste residues generated during the production of juice and pulp, the weight ratio of attapulgite to cocamidopropyl betaine in the conditioning agent is 100:4-8, and the conditioning agent is used in an amount of 5-10% of the weight of the mixed residue. Attapulgite has a unique nanostructure and strong adsorption properties, which can maintain the moisture balance of the fermentation pile during the mixture and enzymatic hydrolysis process, promote microbial fermentation, increase the fermentation maturity rate of the mixture, reduce the loss of nutrients such as nitrogen and minerals, increase the degree of maturity of organic fertilizer, improve the quality and fertility of the organic fertilizer matrix, and cocamidopropyl betaine is beneficial to promote the degradation of the mixture by enzymes and microorganisms, thereby increasing the degree of maturity of the mixture.
[0007] Preferably, in the above-mentioned method for recycling waste residues generated during the production of juice and pulp, in step (3), the amount of pectin lyase used is 0.02-0.05% of the weight of the mixed residue, and the activity is 30,000-100,000 U / g; the amount of laccase used is 0.03-0.08% of the weight of the mixed residue, and the activity is 30,000-100,000 U / g.
[0008] Preferably, in the above-mentioned method for recycling waste residue generated during the production of juice and pulp, the composite fermentation agent is composed of the following components in parts by weight: 5-10 parts of Aspergillus niger, 4-9 parts of Lactobacillus plantarum, 3-8 parts of Azotobacter chlorococcidioides, and 2-4 parts of Geobacillus stearothermophilus. Aspergillus niger in the composite fermentation agent secretes active enzymes such as cellulase and hemicellulase, which efficiently decompose the cellulose in sugarcane bagasse / fruit pomace. Lactobacillus plantarum ferments sugars to produce organic acids such as lactic acid, which softens the fiber. Organic acids and microorganisms compete with each other to inhibit harmful bacteria and reduce odor. Bacillus stearothermophilus secretes heat-resistant lipase to decompose lipid substances in the pomace, maintain high temperature, inhibit miscellaneous bacteria, kill pathogens, and assist in the degradation of cellulase. Brown-globose nitrogen-fixing bacteria proliferate and fix nitrogen, and microbial metabolism produces active substances such as amino acids and vitamins. At the same time, microbial residues are enriched to form stable humus. The synergistic effect between the four bacteria in the composite fermentation agent efficiently ferments and degrades sugarcane bagasse and fruit pomace, preparing an excellent solid organic fertilizer matrix. The solid organic fertilizer matrix can increase soil organic matter, promote crops, and prevent and reduce the occurrence of crop diseases.
[0009] Preferably, in the above-mentioned method for recycling waste residues generated during the production of juice and pulp, the composite fermentation agent is 0.5-1.5% of the weight of the mixed residues.
[0010] Preferably, in the above-mentioned method for recycling waste residues generated during the production of juice and pulp, in step (5), the amount of Aspergillus oryzae used is 0.1-0.4% by weight of the Wogan waste liquid, and the amount of brewer's yeast used is 0.5-0.9% by weight of the Wogan waste liquid.
[0011] Preferably, in the waste residue recycling method in the production process of fruit juice and fruit paste, in step (5), the mixture is obtained by adding sea algae oligosaccharide and solanum nigrum powder into the waste liquid of wampee, the solanum nigrum powder is obtained by drying and crushing the whole plant of solanum nigrum into powder, the amount of sea algae oligosaccharide is 1-3% of the waste liquid of wampee, the amount of solanum nigrum powder is 5-15% of the waste liquid of wampee, then 0.1-0.4% of aspergillus oryzae and 0.5-0.9% of beer yeast are added into the mixture, and the mixture is fermented at room temperature for 30-40 days, then the filtrate obtained by filtration is the enzyme liquid fertilizer, and the filter residue can be used as the solid substrate of fermented organic fertilizer.
[0012] Preferably, in the waste residue recycling method in the production process of fruit juice and fruit paste, the preparation of the composite fermentation agent comprises: aspergillus niger, lactobacillus plantarum, azotobacter paspali and geobacillus stearothermophilus are respectively amplified according to the growth characteristics of each strain, and when the concentration of each strain reaches 4-8×10 9 Cfu / g, the mixed fermentation agent is prepared by mixing according to weight parts.
[0013] On the other hand, the application also provides an organic fertilizer prepared by the above method, which comprises a solid organic fertilizer substrate and an enzyme liquid fertilizer.
[0014] On the other hand, the application also provides the application of the above organic fertilizer in pepper planting, wherein the solid organic fertilizer substrate is used as a pepper seedling cultivation substrate, and the enzyme liquid fertilizer is applied in the process of pepper seedling cultivation, so that the emergence rate of pepper is improved, and the occurrence of diseases and pests in the process of pepper seedling cultivation is reduced.
[0015] Compared with the prior art, the application has the following beneficial effects: 1. The waste residue recycling method in the production process of fruit juice and fruit paste of the application can improve the treatment efficiency by adding a regulator, enzyme pretreatment and composite fermentation agent to ferment and treat the waste residue generated in the production process of fruit juice and fruit paste, can efficiently convert the waste residue into excellent organic fertilizer, can realize the full recycling of waste residue, will not produce new waste, can reduce resource waste, reduce environmental pollution, and is conducive to sustainable development.
[0016] 2. The present application adds a regulator consisting of attapulgite and cocamide propyl betaine to the mixed slag. Attapulgite has a unique nanostructure and strong adsorption, which can maintain the moisture balance of the fermentation pile during the mixing of materials and enzymatic hydrolysis, promote microbial fermentation, increase the fermentation and maturity rate of the mixed materials, reduce the loss of nitrogen, minerals and other nutrients, improve the degree of organic fertilizer maturity, and improve the quality and fertility of the organic fertilizer substrate. The addition of cocamide propyl betaine can further promote the degradation of enzymes and microorganisms to the mixed materials, improve the degree of maturity of the mixed materials, and obtain high-quality solid organic fertilizer substrate.
[0017] 3. The present application first performs enzymatic pretreatment on the waste slag to degrade pectin, break the structure of lignin, reduce the coating of pectin on the fiber slag, and break the tight combination of cellulose, hemicellulose and lignin, which is beneficial to the subsequent effective degradation of microorganisms. Then, the mixed material after enzymatic pretreatment is fermented and matured by using a complex fermentation agent to efficiently decompose cellulose in sugarcane bagasse / pulp, generate lactic acid and other organic acids by fermenting sugars, inhibit harmful bacteria, reduce odor production, reduce the loss of nutrients, and improve the content of amino acids, vitamins and other active substances, thereby obtaining a solid organic fertilizer substrate with high fertility. At the same time, the waste liquid is fermented to form an enzyme liquid fertilizer, and the waste generated during the production of fruit juice and pulp is fully recycled.
[0018] 4. The solid organic fertilizer substrate and enzyme liquid fertilizer obtained by fermenting the waste slag generated during the production of fruit juice and pulp can provide comprehensive and balanced nutrition for crop growth and prevent and reduce the occurrence of crop diseases. The use of the present application in the cultivation of pepper seedlings can improve the emergence rate of pepper and reduce the incidence of pepper seedling sudden death disease. DETAILED DESCRIPTION
[0019] The specific embodiments of the present application are described in detail below, but it should be understood that the scope of protection of the present application is not limited by the specific embodiments. Unless otherwise defined, all professional terms used herein have the same meaning as generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing the specific embodiments and are not intended to limit the scope of protection of the present application. Unless otherwise specified, the various raw materials, reagents, instruments and equipment used in the present application can be purchased from the market or prepared by existing methods.
[0020] Example 1 A method for recycling waste slag generated during the production of fruit juice and pulp, comprising the following steps: (1) crushing the first-pressed wampee peel slag and wampee pulp slag, then performing secondary pressing and filtration to obtain wampee waste slag and wampee waste liquid; (2) The sugarcane residue is cut into short sections less than 2 cm, the cut sugarcane residue and the waste residue of wampee obtained in step (1) are mixed according to a weight ratio of 1:3 to obtain mixed residue, 7.5% of the adjusting agent by weight of the mixed residue is added for stirring and mixing, the adjusting agent is composed of attapulgite and cocamide propyl betaine according to a weight ratio of 100:6, and a mixture is obtained; (3) Pectin lyase and laccase are added into the mixture obtained in step (2) for mixing, the amount of pectin lyase is 0.03% by weight of the mixed residue, and the activity is 50,000 U / g; the amount of laccase is 0.05% by weight of the mixed residue, and the activity is 50,000 U / g, then enzymatic pretreatment is carried out at a temperature of 40℃ for 5 hours, and a pretreated mixture is obtained; (4) Then 1% of the compound fermentation agent by weight of the mixed residue obtained in step (3) is added into the mixture, the compound fermentation agent is composed of Aspergillus niger 7.5 parts, Lactobacillus plantarum 6 parts, Azotobacter chroococcum 5 parts and Geobacillus stearothermophilus 3 parts by weight, and composting fermentation treatment is carried out for 5 days for turning over, and the composting continues to ferment for 10 days, and a solid organic fertilizer base is obtained; The preparation of the compound fermentation agent comprises: Aspergillus niger, Lactobacillus plantarum, Azotobacter chroococcum and Geobacillus stearothermophilus are respectively amplified according to the growth characteristics of each strain, when the concentration of each strain reaches 6×10 9 cfu / g, the mixed fermentation agent is obtained by mixing according to weight parts; (5) The wampee waste liquid obtained in step (1) is mixed with 0.25% of Aspergillus oryzae and 0.7% of beer yeast by weight of the wampee waste liquid, and natural fermentation is carried out for 30 days, and the filtrate obtained by filtration is the enzyme liquid fertilizer.
[0021] Example 2 A method for recycling waste residue generated in a fruit juice and fruit pulp production process, comprising the following steps: (1) The primary pressed wampee peel residue and wampee fruit residue are crushed, and then secondary pressing, filtering are carried out to obtain wampee waste residue and wampee waste liquid; (2) The sugarcane residue is cut into short sections less than 2 cm, the cut sugarcane residue and the waste residue of wampee obtained in step (1) are mixed according to a weight ratio of 1:2 to obtain mixed residue, 5% of the adjusting agent by weight of the mixed residue is added for stirring and mixing, the adjusting agent is composed of attapulgite and cocamide propyl betaine according to a weight ratio of 100:8, and a mixture is obtained; (3) Pectin lyase and laccase are added into the mixture obtained in step (2) for mixing, the amount of pectin lyase is 0.02% by weight of the mixed residue, and the activity is 50,000 U / g; the amount of laccase is 0.07% by weight of the mixed residue, and the activity is 50,000 U / g, then enzymatic pretreatment is carried out at a temperature of 40℃ for 5 hours, and a pretreated mixture is obtained; (4) Then 1.5% of the mixed slag weight of the compound fermentation agent is added to the mixture obtained in step (3), and the compound fermentation agent is composed of 9 parts of Aspergillus niger, 5 parts of Lactobacillus plantarum, 3 parts of Azotobacter chroococcum, and 4 parts of Geobacillus stearothermophilus by weight, and the compost fermentation treatment is carried out for 5 days for turning over, and the compaction continues to ferment for 10 days to obtain the solid organic fertilizer base; The preparation of the compound fermentation agent includes: Aspergillus niger, Lactobacillus plantarum, Azotobacter chroococcum, and Geobacillus stearothermophilus are respectively amplified according to the growth characteristics of each strain, and when the strain concentration reaches 6×10 9 cfu / g, the mixed fermentation agent is prepared by mixing according to the weight parts; (5) 0.1% of Aspergillus oryzae and 0.9% of beer yeast are added to the waste liquid of the wampe obtained in step (1), and then mixed naturally for 30 days to obtain the filtrate, which is the enzyme liquid fertilizer.
[0022] Example 3 A method for recycling waste slag generated in a fruit juice and fruit pulp production process, comprising the following steps: (1) The first-pressed wampe skin slag and wampe fruit slag are crushed, and then second-pressed, filtered to obtain wampe waste slag and wampe waste liquid; (2) The sugarcane slag is cut into short sections less than 2 cm, and the cut sugarcane slag and the wampe waste slag obtained in step (1) are mixed according to a weight ratio of 1:2-4 to obtain mixed slag, and 7.5% of the weight of the mixed slag of the adjusting agent is added for stirring and mixing, and the adjusting agent is composed of attapulgite and cocamide propyl betaine in a weight ratio of 100:6 to obtain the mixture; (3) Pectinase and laccase are added to the mixture obtained in step (2) for mixing, the amount of pectinase is 0.03% of the weight of the mixed slag, and the activity is 50,000 U / g; the amount of laccase is 0.05% of the weight of the mixed slag, and the activity is 50,000 U / g, and then enzyme hydrolysis pretreatment is carried out at a temperature of 40℃ for 5h to obtain the pretreated mixture; (4) Then 1% of the mixed slag weight of the compound fermentation agent is added to the mixture obtained in step (3), and the compound fermentation agent is composed of 7.5 parts of Aspergillus niger, 6 parts of Lactobacillus plantarum, 5 parts of Azotobacter chroococcum, and 3 parts of Geobacillus stearothermophilus by weight, and the compost fermentation treatment is carried out for 5 days for turning over, and the compaction continues to ferment for 10 days to obtain the solid organic fertilizer base; The preparation of the compound fermentation agent includes: Aspergillus niger, Lactobacillus plantarum, Azotobacter chroococcum, and Geobacillus stearothermophilus are respectively amplified according to the growth characteristics of each strain, and when the strain concentration reaches 6×10 9 cfu / g, the mixed fermentation agent is prepared by mixing according to the weight parts; (5) the waste liquid of the wu citrus obtained in step (1) is mixed with sea algal oligosaccharide and nightshade powder to obtain a mixture, the nightshade powder is obtained by drying and crushing the whole plant of nightshade into powder, the amount of sea algal oligosaccharide is 2% of the waste liquid of the wu citrus, the amount of nightshade powder is 10% of the waste liquid of the wu citrus, then 0.25% of aspergillus oryzae and 0.7% of beer yeast are added to the mixture, and the mixture is fermented at room temperature for 30 days, and then filtered to obtain a filtrate, which is the enzyme liquid fertilizer.
[0023] Comparative Example 1 The difference between the present comparative example and Example 1 is that no adjusting agent is added in step (2).
[0024] Comparative Example 2 The difference between the present comparative example and Example 1 is that the adjusting agent in step (2) is only attapulgite, and the others are the same as in Example 1.
[0025] Comparative Example 3 The difference between the present comparative example and Example 1 is that the adjusting agent in step (2) is only cocamide propyl betaine, and the others are the same as in Example 1.
[0026] Comparative Example 4 The difference between the present comparative example and Example 1 is that sodium lauroyl sarcosinate is used to replace cocamide propyl betaine in the adjusting agent in step (2), and the others are the same as in Example 1.
[0027] Comparative Example 5 The difference between the present comparative example and Example 1 is that no enzymatic pretreatment is performed.
[0028] Comparative Example 6 The difference between the present comparative example and Example 3 is that, in step (5), perilla powder is used to replace nightshade powder, and the perilla powder is obtained by drying and crushing the whole plant of perilla into powder.
[0029] The degradation rates of cellulose, hemicellulose and lignin in the mixed residue in Examples 1-2 and Comparative Examples 1-5 are tested, the content of cellulose is determined by ferrous ammonium sulfate titration, the content of cellulose is determined by ferrous ammonium sulfate titration, and the content of hemicellulose is determined by sodium thiosulfate titration, on the basis of dry matter, and the calculation formula of the degradation rate of cellulose is as follows: Cellulose degradation rate%= (cellulose content in the mixture before treatment-cellulose content in the solid organic fertilizer substrate after treatment) *100 / cellulose content in the mixture before treatment; The calculation formula of the degradation rates of hemicellulose and lignin is similar to that of the degradation rate of cellulose, and the results are shown in Table 1.
[0030] As shown in Table 1, the degradation effect of the waste residue can be obviously improved, and the treatment efficiency of the waste residue can be improved by adding the adjusting agent, the enzymatic pretreatment and the fermentation of the compound fermentation agent.
[0031] Table 1 cellulose, hemicellulose and lignin degradation rate of each group Test Example 1 The solid organic fertilizer matrix prepared in Example 1 and Example 2 is used as a cultivation matrix for pepper seedlings, and peat soil and vermiculite are mixed in a weight ratio of 1:1 to serve as a control matrix, and the test pepper variety is Nandanchaogaojiao, and a control group, Example 1 group and Example 2 group are set up, each with 3 repetitions, and standard plug planting is used, with 64 holes in each group, and the Example matrix is loaded into the plug, and water is poured through, and the pepper seeds are soaked for 12 hours before sowing, and germination is induced for 48 hours, and the seedlings are cultivated in a greenhouse. The germination rate is investigated 7 days after sowing, and the stem diameter of the stable seedlings is investigated after 20 days, and the results are shown in Table 2. Germination rate = (germinated seeds / total number of seeds sown) * 100%.
[0032] As can be seen from Table 2, the solid organic fertilizer matrix prepared in the present application can improve the germination rate of pepper seedlings and promote the growth of pepper seedlings.
[0033] Table 2 Pepper germination rate of each treatment group Test Example 2 The solid organic fertilizer matrix of Example 1 is used as a seedling cultivation matrix for pepper seedlings, and peat soil and vermiculite are mixed in a weight ratio of 1:1 to serve as a control matrix, and the old peat soil with damping-off is added to the solid organic fertilizer matrix and the control matrix to obtain the final cultivation matrix, and 5 treatment groups are set up, and the sowing treatment method is the same as in Example 1, and on the 10th day after sowing, the treatment is carried out in the manner shown in Table 2, and the enzyme liquid fertilizer is diluted 20 times with water for irrigation, and 10 ml is irrigated per hole, and after irrigation, the control group is irrigated with 10 ml of clean water, and the incidence of damping-off of pepper seedlings is investigated on the 10th day after irrigation. Incidence rate = (diseased pepper seedlings * 100%) / total number of pepper seedlings.
[0034] Table 2 Treatment method of each treatment group Table 3 is the incidence rate of each treatment group, and pepper damping-off is a major disease of pepper seedlings, which often causes seed rot, seedling damping-off, and even death in severe cases. As can be seen from the results, the solid organic fertilizer matrix of the present application can reduce the incidence of pepper seedling damping-off to a certain extent, and the enzyme liquid fertilizer obtained by fermentation of waste residue, nightshade and seaweed oligosaccharide can greatly reduce the incidence of pepper damping-off.
[0035] Table 3 Incidence rate of each treatment group The foregoing description of specific exemplary embodiments of the application has been presented for the purposes of illustration and description. It is not intended to be exhaustive or to limit the application to the precise forms disclosed, and obviously many modifications and variations are possible in light of the above teaching. It is intended that the scope of the application be limited not with this detailed description, but rather by the claims appended hereto.
Claims
1. A method for recycling waste residues generated during the production of juice and pulp, characterized in that: The following steps are involved: (1) crushing the Wogan peel residue and Wogan pomace obtained by the primary squeezing, and then performing secondary squeezing and filtering to obtain Wogan waste residue and Wogan waste liquid; (2) chopping the bagasse into short sections less than 2 cm, mixing the chopped bagasse with the Wogan waste residue obtained in step (1) in a weight ratio of 1:2 to 4 to obtain a mixed residue, adding a regulator and stirring and mixing, wherein the regulator consists of attapulgite and cocamidopropyl betaine to obtain a mixed material; (3) adding pectin lyase and laccase to the mixed material obtained in step (2) and mixing them, and then performing enzymatic pretreatment at a temperature of 25-50° C. for 4-6 hours to obtain a pretreated mixed material; (4) adding a composite fermentation agent to the mixed material obtained by the pretreatment in step (3), wherein the composite fermentation agent includes Aspergillus niger, Lactobacillus plantarum, Nitrogen-fixing Bacterium chlorophyllus, and Geobacillus stearothermophilus, and composting and fermenting the mixed material for 5 to 7 days, turning the compost, and continuing the composting and fermentation for 10 to 15 days to obtain a solid organic fertilizer matrix; (5) Adding Aspergillus oryzae and brewer's yeast to the citrus waste liquid obtained in step (1) and fermenting to obtain enzyme liquid fertilizer.
2. The method for recycling waste residue generated in the production process of juice and pulp according to claim 1, characterized in that: The weight ratio of attapulgite to cocamidopropyl betaine in the regulator is 100:4-8, and the amount of the regulator is 5-10% of the weight of the mixed slag.
3. The method for recycling waste residue generated in the production process of juice and pulp according to claim 1, characterized in that: In the step (3), the amount of pectin lyase used is 0.02-0.05% of the weight of the mixed residue, and the activity is 30,000-100,000 U / g; the amount of laccase used is 0.03-0.08% of the weight of the mixed residue, and the activity is 30,000-100,000 U / g.
4. The method for recycling waste residue generated in the production process of juice and pulp according to claim 1, characterized in that: The composite fermentation bacteria agent is composed of the following components in parts by weight: 5-10 parts of Aspergillus niger, 4-9 parts of Lactobacillus plantarum, 3-8 parts of Azotobacter chlorosphaerus, and 2-4 parts of Geobacillus stearothermophilus.
5. The method for recycling waste residue generated in the production process of juice and pulp according to claim 1, characterized in that: The composite fermentation agent accounts for 0.5-1.5% of the weight of the mixed slag.
6. The method for recycling waste residue generated in the production process of juice and pulp according to claim 1, characterized in that: In the step (5), the amount of Aspergillus oryzae is 0.1-0.4% of the weight of the Wogan waste liquid, and the amount of brewer's yeast is 0.5-0.9% of the weight of the Wogan waste liquid.
7. The method for recycling waste residue generated in the production process of juice and pulp according to claim 1, characterized in that: In the step (5), seaweed oligosaccharides and nightshade powder are added to the Wogan waste liquid and mixed to obtain a mixture, wherein the amount of seaweed oligosaccharides is 1-3% of the Wogan waste liquid, and the amount of nightshade powder is 5-15% of the Wogan waste liquid, and then 0.1-0.4% of Aspergillus oryzae and 0.5-0.9% of brewer's yeast are added based on the weight of the mixture, and fermented at room temperature for 30-40 days, and then filtered to obtain a filtrate, which is the enzyme liquid fertilizer.
8. The method for recycling waste residue generated in the production process of juice and pulp according to claim 4, characterized in that: The preparation of the composite fermentation agent comprises: amplifying and culturing Aspergillus niger, Lactobacillus plantarum, Nitrogen-fixing Bacterium chlorophyllum, and Geobacillus stearothermophilus according to the growth characteristics of each strain, and when the strain concentration reaches 4-8×10 9 cfu / g, and mixed according to weight to prepare the mixed fermentation agent.
9. an organic fertilizer prepared by the method according to any one of claims 1 to 8, characterized in that, The organic fertilizer comprises a solid organic fertilizer matrix and an enzyme liquid fertilizer.
10. The use of the organic fertilizer in pepper planting as claimed in claim 9, characterized in that: The solid organic fertilizer matrix is used as a pepper seedling cultivation matrix, and enzyme liquid fertilizer is applied during the pepper seedling cultivation process.