Adjustable biological fluorescence imaging lighting device and system based on fiber bragg grating

Abstract

The invention provides an adjustable bioluminescence imaging lighting device based on a fiber Bragg grating. The adjustable bioluminescence imaging lighting device comprises a light source; the first lens unit is used for converting light rays emitted by the light source into parallel light rays; the rotary light splitting unit is used for enabling all the parallel light rays to penetrate or splitting the parallel light rays into transmission light and reflected light; the second lens unit is used for transmitting the focused light and inputting the focused light to the first fiber bragg grating through the first coupler; the first fiber bragg grating is used for reflecting the narrow-band light of the first preset wave band and outputting the narrow-band light through the output end of the first coupler; the third lens unit is used for focusing the reflected light and inputting the focused reflected light to a second fiber bragg grating through a second coupler; the second fiber bragg grating is used for reflecting narrow-band light of a second preset wave band and outputting the narrow-band light through the output end of the second coupler; and the third coupler is used for mixing the input light rays of the first / second preset wave bands and then outputting the mixed light rays to the light source input end of the endoscope. The device can be used for clinical diagnosis and detection.

Description

Technical Field

[0001] The present invention relates to the technical field of endoscope imaging, and in particular to an adjustable biological fluorescence imaging lighting device and system based on fiber Bragg grating. Background Art

[0002] Since most advanced cancer lesions require surgical resection, early detection and prevention of cancer are essential. Accurate observation of the color changes and morphological structure of the mucosa is essential for diagnosing potential malignancies. Narrowband imaging (NBI) is an endoscopic technique that uses special optical filters to narrow the light bandwidth to enhance visualization of the mucosal surface and microvasculature. The NBI system uses two narrowband wavelengths, 415 nm and 540 nm, corresponding to the absorption peak of hemoglobin. Therefore, compared with conventional white light imaging, NBI can more clearly visualize fine blood vessels in the epithelium or mucosal layer, such as capillaries. The bandwidth of the filter is typically tens of nanometers, while the reflection bandwidth of the fiber Bragg grating is usually less than 1 nm. Based on the fiber Bragg grating, narrowband light with a narrower spectral width can be obtained. Therefore, the development of a device based on the fiber Bragg grating for endoscopic narrowband imaging has broad application prospects and clinical significance.

[0003] On the other hand, some diseases, such as Wilson's disease, require urine copper testing for diagnostic criteria. Using light in a specific wavelength range in conjunction with a fluorescent probe can provide more accurate detection. This necessitates the development of a narrowband imaging device using light in this specific wavelength range and a compatible fluorescent probe (reference paper: Synthesis and Spectral Response of Novel Benzothiazole Derivatives; Zhu Yanru). Summary of the Invention

[0004] Based on the above background, the present invention provides an adjustable bioluminescence imaging lighting device and system based on fiber Bragg grating, which specifically adopts the following technical solutions: The first aspect of the present invention provides an adjustable bioluminescence imaging lighting device based on a fiber Bragg grating, comprising: A light source configured to emit light within a certain wavelength range, wherein the certain wavelength range includes at least a first preset wavelength range and a second preset wavelength range; A first lens unit is placed on the light-emitting side of the light source and is used to transform the light emitted by the light source into parallel light; a rotating light splitting unit, located on the optical path of the parallel light, for transmitting all of the parallel light or splitting the parallel light into transmitted light and reflected light according to a control instruction; a second lens unit, which is placed on the optical path of the transmitted light and is used to focus the parallel transmitted light and then input it into the first fiber Bragg grating through the first coupler; a first fiber Bragg grating, which is used to reflect narrowband light in a first preset wavelength band and output it through an output end of the first coupler; a third lens unit, which is placed on the optical path of the reflected light and is used to focus the parallel reflected light and then input it into the second fiber Bragg grating through the second coupler; a second fiber Bragg grating, which is used to reflect narrowband light in a second preset wavelength band and output it through an output end of the second coupler; The third coupler has two input ends coupled to the output end of the first coupler and the output end of the second coupler respectively, and is used to mix the input light of the first preset band and the light of the second preset band and output them to the light source input end of the endoscope through the output end.

[0005] Furthermore, the narrowband light of the first preset wavelength band is narrowband light with a wavelength of 540 nm, and the narrowband light of the second preset wavelength band is narrowband light with a wavelength of 415 nm.

[0006] Furthermore, the first fiber Bragg grating and the second fiber Bragg grating are respectively packaged by a material having a thermal expansion coefficient greater than a preset value.

[0007] Furthermore, the first fiber Bragg grating and the second fiber Bragg grating are respectively packaged in an aluminum groove, and the reflection wavelength is changed based on the thermal expansion and contraction effect of the aluminum groove caused by temperature changes.

[0008] Furthermore, the change in the reflected wavelength due to the thermal expansion and contraction effect of the aluminum tank caused by temperature change is calculated based on the following formula:

[0009] in, is the thermal expansion coefficient of aluminum, is the effective elastic-optical coefficient, and are the thermo-optical coefficient and thermal expansion coefficient of the fiber Bragg grating, respectively.

[0010] Furthermore, the rotating spectrometer includes a semi-transparent and semi-reflective mirror and a driving mechanism, wherein the driving mechanism is used to drive the semi-transparent and semi-reflective mirror to rotate, and when the semi-transparent and semi-reflective mirror is perpendicular to the parallel light, all the parallel light is transmitted; when the semi-transparent and semi-reflective mirror forms a certain angle with the parallel light, the parallel light is divided into transmitted light and reflected light.

[0011] Furthermore, the third coupler adjusts the ratio of the narrowband light of the first preset wavelength band to the narrowband light of the first preset wavelength band that is finally output based on different coupling ratios.

[0012] A second aspect of the present invention further provides an adjustable bioluminescence imaging illumination system based on a fiber Bragg grating, comprising the adjustable bioluminescence imaging illumination device as described in the first aspect above, and also comprising a fluorescent probe R1 adapted to the adjustable bioluminescence imaging illumination device, wherein the fluorescent probe R1 is synthesized based on the following process: 5-Methylsalicylaldehyde and 2-aminothiophenol were dissolved in N,N-dimethylformamide and heated under reflux to obtain intermediate 1; Intermediate 1 and hexamethylenetetramine are dissolved in trifluoroacetic acid and heated under reflux to react to obtain intermediate 2; Intermediate 2 and rhodamine 101 hydrazide were dissolved in anhydrous ethanol and subjected to heating under reflux, rotary evaporation, precipitation and recrystallization to obtain R1; A 20 μM probe R1 solution was prepared by mixing R1 with ethanol and PBS buffer solution (pH=7.4) at a ratio of 6:4 (v:v).

[0013] The beneficial effects of the present invention are as follows: 1) By using fiber Bragg gratings, the present invention can precisely control the wavelength of narrow-band illumination light so that it corresponds more accurately to the absorption peak of hemoglobin, thereby providing better lighting effects.

[0014] 2) The present invention uses a rotating light splitting unit and a third coupler with different coupling ratios to control the proportion of narrowband light in different preset bands, thereby adapting it to different application scenarios.

[0015] 3) The present invention can further precisely control the narrowband wavelength by encapsulating the fiber Bragg grating in an aluminum groove and changing the reflection wavelength based on the thermal expansion and contraction effect of the aluminum groove.

[0016] 4) The system of the present invention also provides a fluorescent probe R1 adapted to the imaging illumination device, thereby corresponding to the precisely modulated wavelength and providing more accurate detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 Schematic diagram of the composition of an embodiment of an endoscope narrow-band imaging lighting device of the present invention. DETAILED DESCRIPTION

[0018] Embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although certain embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be construed as limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of the present invention. It should be understood that the drawings and embodiments of the present invention are for illustrative purposes only and are not intended to limit the scope of protection of the present invention.

[0019] See also Figure 1The embodiment of the present invention provides an adjustable bioluminescence imaging lighting device based on a fiber Bragg grating, comprising: A light source, comprising a reflector 1 and a light emitting body 2, for emitting light within a certain wavelength range, wherein the wavelength range includes at least a first preset wavelength range and a second preset wavelength range; The first lens unit 3 is placed on the light-emitting side of the light source and is used to transform the light emitted by the light source into parallel light; A rotating light splitting unit 4, which is located on the optical path of the parallel light and is used to transmit all the parallel light or split the parallel light into transmitted light and reflected light according to a control instruction; The second lens unit 5 is placed on the optical path of the transmitted light and is used to focus the parallel transmitted light and then input it into the first fiber Bragg grating 9 through the first coupler 8; a first fiber Bragg grating 9, which is used to reflect narrowband light in a first preset wavelength band and output it through the output end 7 of the first coupler 8; The third lens unit 10 is placed on the optical path of the reflected light and is used to focus the parallel reflected light and then input it into the second fiber Bragg grating 14 through the second coupler 13; A second fiber Bragg grating 14 is used to reflect narrowband light in a second preset wavelength band and output it through the output end 12 of the second coupler; The third coupler 15 has two input ends coupled to the output end of the first coupler and the output end of the second coupler respectively, and is used to mix the input light of the first preset wavelength band and the input light of the second preset wavelength band and output them to the light source input end of the endoscope through the output end.

[0020] As a preferred implementation, in this embodiment, the narrowband light of the first preset wavelength band is narrowband light with a wavelength of 540 nm, and the narrowband light of the second preset wavelength band is narrowband light with a wavelength of 415 nm.

[0021] As a preferred implementation, in this embodiment, the first fiber Bragg grating 9 and the second fiber Bragg grating 14 are respectively packaged by a material having a thermal expansion coefficient greater than a preset value.

[0022] As a preferred embodiment, in this embodiment, the first fiber Bragg grating 9 and the second fiber Bragg grating 14 are respectively packaged in an aluminum groove, and the reflection wavelength is changed based on the thermal expansion and contraction effect of the aluminum groove caused by temperature changes.

[0023] In particular, in this embodiment, the change in the reflected wavelength due to the thermal expansion and contraction effect of the aluminum groove caused by temperature change is calculated based on the following formula:

[0024] in, is the thermal expansion coefficient of aluminum, is the effective elastic-optical coefficient, and are the thermo-optical coefficient and thermal expansion coefficient of the fiber Bragg grating, respectively.

[0025] As a preferred embodiment, in this embodiment, the rotating spectrometer unit includes a semi-transparent and semi-reflective mirror and a driving mechanism, and the driving mechanism is used to drive the semi-transparent and semi-reflective mirror to rotate, and when the semi-transparent and semi-reflective mirror is perpendicular to the parallel light, all the parallel light is transmitted; when the semi-transparent and semi-reflective mirror is at a certain angle to the parallel light, the parallel light is divided into transmitted light and reflected light.

[0026] As a preferred implementation, in this embodiment, the third coupler adjusts the ratio of the narrowband light of the first preset wavelength band finally output and the narrowband light of the first preset wavelength band based on different coupling ratios.

[0027] A second embodiment of the present invention further provides an adjustable bioluminescence imaging illumination system based on a fiber Bragg grating, comprising the adjustable bioluminescence imaging illumination device as described in the first embodiment above, and a fluorescent probe R1 adapted to the adjustable bioluminescence imaging illumination device, wherein the fluorescent probe R1 is synthesized based on the following process: 5-Methylsalicylaldehyde and 2-aminothiophenol were dissolved in N,N-dimethylformamide and heated under reflux to obtain intermediate 1; Intermediate 1 and hexamethylenetetramine are dissolved in trifluoroacetic acid and heated under reflux to react to obtain intermediate 2; Intermediate 2 and rhodamine 101 hydrazide were dissolved in anhydrous ethanol and subjected to heating under reflux, rotary evaporation, precipitation and recrystallization to obtain R1; A 20 μM probe R1 solution was prepared by mixing R1 with ethanol and PBS buffer solution (pH=7.4) at a ratio of 6:4 (v:v).

[0028] Here's how it works: Metal ions such as and The presence of may cause the spiro ring of the rhodamine lactone in the fluorescent probe R1 to become open, thereby causing a significant change in the R1 solution, with the color changing from the initial colorless to purple. From the spectral characteristics, its ultraviolet absorption peak moves toward the long-wave direction (red-shift direction).

[0029] During the test, the treated sample to be tested (such as urine, etc. needs to be diluted in PBS buffer) is taken and R1 is added to a solution of ethanol: sample to be tested = 6:4 (v:v). If the color of the solution changes significantly (from colorless to purple), and the narrow-band imaging lighting device of the present invention is used to excite ( =540nm) and the solution produces orange fluorescence (600nm), which means that the sample to be tested is likely to contain or The intensity of fluorescence reflects the concentration of metal ions. If the solution produces fluorescence, the wavelength can be fine-tuned by changing the temperature of the aluminum tank where the fiber Bragg grating is located to obtain a fluorescence intensity more suitable for observation.

[0030] The embodiments of the present invention are intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the embodiments of the present invention should be included in the scope of protection of the present invention.

Claims

1. An adjustable bioluminescence imaging lighting device based on fiber Bragg grating, characterized in that: include: A light source configured to emit light within a certain wavelength range, wherein the certain wavelength range includes at least a first preset wavelength range and a second preset wavelength range; A first lens unit is placed on the light-emitting side of the light source and is used to transform the light emitted by the light source into parallel light; a rotating light splitting unit, located on the optical path of the parallel light, for transmitting all of the parallel light or splitting the parallel light into transmitted light and reflected light according to a control instruction; a second lens unit, which is placed on the optical path of the transmitted light and is used to focus the parallel transmitted light and then input it into the first fiber Bragg grating through the first coupler; a first fiber Bragg grating, which is used to reflect narrowband light in a first preset wavelength band and output it through an output end of the first coupler; a third lens unit, which is placed on the optical path of the reflected light and is used to focus the parallel reflected light and then input it into the second fiber Bragg grating through the second coupler; a second fiber Bragg grating, which is used to reflect narrowband light in a second preset wavelength band and output it through an output end of the second coupler; The third coupler has two input ends coupled to the output end of the first coupler and the output end of the second coupler respectively, and is used to mix the input light of the first preset band and the light of the second preset band and output them to the light source input end of the endoscope through the output end.

2. The adjustable bioluminescence imaging lighting device based on fiber Bragg grating according to claim 1, characterized in that: The narrowband light of the first preset wavelength band is narrowband light with a wavelength of 540 nm, and the narrowband light of the second preset wavelength band is narrowband light with a wavelength of 415 nm.

3. The adjustable bioluminescence imaging lighting device based on fiber Bragg grating according to claim 1, characterized in that: The first fiber Bragg grating and the second fiber Bragg grating are respectively packaged by a material having a thermal expansion coefficient greater than a preset value.

4. The adjustable bioluminescence imaging lighting device based on fiber Bragg grating according to claim 3, characterized in that: The first fiber Bragg grating and the second fiber Bragg grating are respectively packaged in an aluminum groove, and the reflection wavelength changes based on the thermal expansion and contraction effect of the aluminum groove caused by temperature changes.

5. The adjustable bioluminescence imaging lighting device based on fiber Bragg grating according to claim 4, characterized in that: The change in the reflected wavelength due to the thermal expansion and contraction effect of the aluminum tank caused by temperature change is calculated based on the following formula: ； in, is the thermal expansion coefficient of aluminum, is the effective elastic-optical coefficient, and are the thermo-optical coefficient and thermal expansion coefficient of the fiber Bragg grating, respectively.

6. The adjustable bioluminescence imaging lighting device based on fiber Bragg grating according to claim 1, characterized in that: The rotating light splitting unit includes a semi-transparent and semi-reflective mirror and a driving mechanism, wherein the driving mechanism is used to drive the semi-transparent and semi-reflective mirror to rotate, and when the semi-transparent and semi-reflective mirror is perpendicular to the parallel light, all the parallel light is transmitted; when the semi-transparent and semi-reflective mirror forms a certain angle with the parallel light, the parallel light is divided into transmitted light and reflected light.

7. The adjustable bioluminescence imaging lighting device based on fiber Bragg grating according to claim 1, characterized in that: The third coupler adjusts the ratio of the narrowband light of the first preset wavelength band to the narrowband light of the first preset wavelength band finally output based on different coupling ratios.

8. An adjustable bioluminescence imaging illumination system based on fiber Bragg grating, characterized in that: The device comprises the adjustable bioluminescence imaging lighting device according to any one of claims 1 to 7, and further comprises a fluorescent probe R1 adapted to the adjustable bioluminescence imaging lighting device, wherein the fluorescent probe R1 is synthesized based on the following process: 5-Methylsalicylaldehyde and 2-aminothiophenol were dissolved in N,N-dimethylformamide and heated under reflux to obtain intermediate 1; Intermediate 1 and hexamethylenetetramine are dissolved in trifluoroacetic acid and heated under reflux to react to obtain intermediate 2; Intermediate 2 and rhodamine 101 hydrazide were dissolved in anhydrous ethanol and subjected to heating under reflux, rotary evaporation, precipitation and recrystallization to obtain R1; A 20 μM probe R1 solution was prepared by mixing R1 with ethanol and PBS buffer solution (pH=7.4) at a ratio of 6:4 (v:v).

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