A fossil-like medicinal material, its preparation method and uses

Abstract

This invention belongs to the field of traditional Chinese medicine preparation, specifically disclosing a method for preparing and using a fossil-like medicinal material. This invention uses mineralized tissues such as bones, teeth, and horns of mammals like cattle, horses, deer, and sheep as raw materials, and processes them through mineral phase transformation and isostatic pressing to produce a fossil-like medicinal material. Based on the principle of natural mineralization of biological bones, the resulting fossil-like medicinal material has the same structure as natural fossil medicinal materials. Animal experiments have verified that natural dragon bone and the fossil-like medicinal material of this invention have comparable calming and sedative effects, possessing practical application value.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation, specifically to a method for preparing and using a fossil-like medicinal material. Background Technology

[0002] Fossils are the remains of ancient animals, traces of their activities, and small organic molecules of biological origin preserved in rocks through natural processes. They are mainly divided into: body fossils, trace fossils, chemical fossils, and special cross-types (amber fossils). Among them, biological skeletal fossils, such as dragon bones, dragon teeth, and dragon horns, hold an important place in traditional Chinese medicine.

[0003] Dragon bones are fossilized skeletons of ancient mammals, typically from animals such as three-toed horses, rhinoceroses, deer, cattle, and elephants. In Traditional Chinese Medicine (TCM), they are used to calm the mind, soothe the nerves, and suppress yang, and are often used to treat insomnia, palpitations, and epilepsy. Dragon teeth are fossilized teeth from ancient large mammals such as elephants, rhinoceroses, and three-toed horses. They also have calming and sedative effects in TCM, but are considered to be more potent than dragon bones. They are often used to treat epilepsy, mania, and palpitations. Dragon horns are fossilized horns from ancient large mammals such as elephants and rhinoceroses. They also have calming and sedative effects in TCM, but are used less frequently, mainly because horn fossils are relatively rare. These fossil medicinal materials are non-renewable resources and are becoming increasingly scarce. They are protected by national laws and regulations, and their use is strictly limited. Research on alternatives is currently stagnant. Therefore, developing alternatives to fossil medicinal materials is of great significance in order to strengthen the protection of fossil resources while addressing resource limitations in their clinical use and breaking through key bottlenecks hindering the development of TCM.

[0004] In recent years, with the deepening research on fossil-based medicinal materials, their chemical composition and pharmacological effects have been further elucidated. Based on their compositional and forging analyses, some researchers have explored the development of substitutes, such as searching for resins and their processed products with similar components as potential effective alternatives to amber. However, there is currently no research on imitation fossil-based medicinal materials that can replace dragon bones, dragon teeth, and dragon horns. Summary of the Invention

[0005] To achieve the above objectives, the present invention provides a method for preparing imitation fossil-like medicinal materials, which includes the following steps:

[0006] The mineralized tissue of mammals, which contains no organic matter, is subjected to mineral phase transformation and isostatic pressing to achieve compaction.

[0007] Among them, the mineral phase transformation involves cutting or crushing the mineralized tissue that does not contain organic matter, placing it in a mineralizing liquid, stirring under high pressure and heat, and then sintering and solidifying it.

[0008] Isostatic compaction involves pressing the mineralized structure after the mineral phase transformation in an isostatic press.

[0009] Furthermore, the mineral phase transformation includes the following steps:

[0010] Take a mineralized tissue without organic matter, cut or crush it, place it in a mineralization liquid, and keep it heated and stirred for 2 to 14 days under the conditions of pressure 1-10 MPa, rotation speed 100-200 rpm / min and temperature 50-80℃. Take out the mineralized tissue, pre-sinter it, and then sinter it to obtain the product.

[0011] The pieces are cut or crushed into 0.3-3 cm pieces;

[0012] Furthermore, the mass-to-volume ratio of the mineralized tissue to the mineralizing solution is 1 kg : 3~10 L; the mineralizing solution is a mixed solution with a pH of 9-11 composed of any three or more of sodium carbonate, sodium bicarbonate, sodium hydroxide, calcium hydroxide, and soil water extract.

[0013] The pre-sintering temperature is 200~300 ℃, and the time is 3~4 h; the sintering is performed 1~2 times, with each time the temperature is 500~700 ℃ and the time is 0.5~2 h;

[0014] Each liter of the soil-water extract is equivalent to 0.5 to 1.5 kg of soil; the soil includes loess.

[0015] Furthermore, the mass-to-volume ratio of the mineralized tissue to the mineralizing solution is 1 kg : 10 L; the mineralizing solution is a mixed solution with a pH of 10.6, composed of sodium carbonate, sodium bicarbonate, calcium hydroxide, and soil water extract.

[0016] The heat preservation stirring was carried out at a pressure of 3 MPa, a rotation speed of 100 rpm / min, a temperature of 70 ℃, and a time of 7 days.

[0017] The pre-sintering temperature is 300 ℃ and the time is 3 h; the sintering is performed once at a temperature of 600 ℃ for 2 h.

[0018] Furthermore, the isostatic compaction includes the following steps:

[0019] Mineralized tissue blocks that have undergone mineral phase transformation are taken, mixed with bone meal or soil, filled into molds, and pressed in an isostatic press.

[0020] The bone powder is a powder of mineralized tissue that has undergone mineral phase transformation.

[0021] Furthermore, the bone meal or soil is 1-15 times the amount of the mineralized tissue;

[0022] The isostatic press is a cold isostatic press and / or a hot isostatic press;

[0023] The pressure of the cold isostatic press is 100-500 MPa, and the pressing time is 0.2-2 h;

[0024] The hot isostatic press has a pressure of 100-250 MPa, a temperature of 100-300°C, and a pressing time of 0.2-2 h.

[0025] Furthermore, the bone meal or soil is 15 times the amount of mineralized tissue;

[0026] The isostatic press is a cold isostatic press;

[0027] The cold isostatic press has a pressure of 250 MPa and a pressing time of 0.5 h.

[0028] Furthermore, the soil is soil from the original habitat of natural keel bones or keel teeth; the soil includes loess.

[0029] Furthermore, the mammals include cattle, horses, deer, donkeys, sheep, and pigs; the mineralized tissues are bones, teeth, and horns.

[0030] This invention also provides a fossil-like medicinal material, which is prepared by the aforementioned preparation method;

[0031] The imitation fossil medicinal materials include imitation dragon bones and imitation dragon teeth;

[0032] The mass percentage of calcium and phosphorus in the imitation keel and imitation keel teeth shall not be less than 2.2%.

[0033] Finally, this invention provides the use of the aforementioned fossil-like medicinal materials in the preparation of sedative and tranquilizing drugs.

[0034] The "mineralized tissue without organic matter" mentioned in this invention refers to biological tissues containing minerals (such as calcium, phosphorus, etc.) and forming hard structures, such as bones, teeth, and horn bones; these biological tissues do not produce black smoke or burnt smell when heated to 250°C; they are not stained red when soaked in Sudan Red solution; and when mixed with NaOH, heated to boiling, and CuSO4 solution is added dropwise, they do not turn blue.

[0035] In its preliminary research, this invention focused on the composition and structure of biological skeletons: the inorganic components of biological skeletons are mainly crystalline hydroxyapatite (Ca). 5 The [PO4]3(OH) group is distributed in organic matter. Although it is known that bones contain some carbonate ions, most researchers still name the bioapatite in bones as hydroxyapatite. Currently, based on [CO3]... 2-Replacement can be categorized into three types: Type A (carbonate ions occupy hydroxyl sites), Type B (carbonate ions occupy phosphate sites), and Type AB (carbonate ions occupy both hydroxyl and phosphate sites). The transformation of biological remains into fossils under natural conditions involves three main processes: biological, chemical, and physical. During burial, biological processes decompose most of the organic matrix in the remains, leaving behind numerous micron- and even nanometer-sized pores, capillaries, and partially blocked channels of the original bone structure, ultimately preserving the inorganic framework. Bones in contact with soil undergo rapid and extensive chemical changes, including increased apatite crystallinity and the transformation into a thermodynamically more stable apatite phase. At the microscopic level, the cell parameter 'a' in bioapatite gradually decreases while 'c' gradually increases. This change is similar to that of artificially synthesized Type B replacement, further suggesting the possible presence of high levels of Type B carbon-hydroxyapatite in skeletal fossils. Due to the loss of organic matter creating pores, various ions from the surrounding environment gradually deposit in the bone voids, with calcite being the most common mineral filler in skeletal fossils. When bones are buried underground for a long time and subjected to continuous physical compression, their density increases further.

[0036] These processes ultimately result in fossil remains such as keel bones possessing inorganic, hygroscopic, dense, and structurally ordered characteristics. Inorganicity refers to the fact that keel bones, as fossilized skeletons, contain trace amounts or no organic matter. Hygroscopicity refers to the strong suction of keel bones, mainly related to the microporous structure of biological skeletons. Weathering creates new fissures, expands and connects existing pores, enhancing overall hygroscopicity. Density refers to the fact that keel bones have a higher density than biological skeletons. Biological skeletons, buried underground, gradually close under static pressure, significantly increasing their density. The contact points of bone particles dissolve under high stress, and the dissolved substances recrystallize in the pores, further filling them. The deeper and longer the burial time, the stronger the compaction and the higher the density. Structural order refers to the fact that keel bones have a higher degree of crystallinity than biological skeletons. The decomposition of organic matter in the skeleton forms a large number of honeycomb-like pores, increasing the specific surface area. The original hydroxyapatite is replaced by a more stable and highly crystalline apatite phase. At the same time, groundwater recrystallizes to produce new minerals—calcium phosphate in the skeleton is replaced by calcite and other minerals, increasing order. The longer the burial time, the higher the order.

[0037] This invention addresses the four characteristics of the aforementioned fossil remains by innovatively employing mineral phase transformation and isostatic pressing techniques to treat mineral tissues lacking organic matter, accelerating the fossilization process and promoting the transformation of biological skeletons into skeletal fossils. The mineral phase transformation technique utilizes the principle that biological skeletons, in a high-pH, carbonate-rich environment, promote the recrystallization of apatite, increasing B-type substitution and forming larger crystals. High concentrations of carbonates, alkaline substances, and loess are added, reacting chemically with the biological skeleton to increase B-type substitution and the formation of secondary minerals. Further high-temperature calcination promotes recrystallization and increases structural order. The isostatic pressing technique applies isotropic pressure uniformly to the biological skeleton using an isostatic press, significantly improving density. High pressure promotes dislocation slip and climb, potentially reducing dislocation density (through dynamic recovery) or inducing recrystallization. Simultaneously, under high-temperature conditions, substances are more prone to recrystallization, forming more stable phases.

[0038] Naturally formed fossil medicinal materials require tens of thousands of years to form. This invention, through analysis and research of fossil medicinal materials and biological skeletons, and based on the principles of natural mineralization of biological skeletons, obtains a method for preparing imitation fossil medicinal materials. The resulting medicinal materials have the same structure as naturally formed fossil medicinal materials, making it possible for the quality and efficacy of the accelerated mineralization imitation fossil medicinal materials to be consistent with those of natural fossil medicinal materials. Animal experiments have verified that natural dragon bone and the dragon bone imitation produced by this invention have comparable calming and sedative effects, demonstrating practical application value.

[0039] Obviously, based on the above description of the present invention, and according to common technical knowledge and conventional methods in the field, various other modifications, substitutions or alterations can be made without departing from the basic technical concept of the present invention.

[0040] The following detailed embodiments further illustrate the above-described content of the present invention. However, this should not be construed as limiting the scope of the present invention to the following examples. All technologies implemented based on the above-described content of the present invention fall within the scope of the present invention. Attached Figure Description

[0041] Figure 1 Flowchart for the preparation of fossil-based medicinal materials;

[0042] Figure 2 X-ray characteristic spectrum of natural keel;

[0043] Figure 3 X-ray characteristic spectrum of the imitation fossil-like medicinal material prepared in Example 1;

[0044] Figure 4 This represents the total distance traveled within the open field;

[0045] Figure 5 The value represents the MDA content in mouse brain tissue. Detailed Implementation

[0046] The raw materials, reagents and equipment used in the specific embodiments of the present invention are all purchased commercially. The preparation method of the loess water extract is as follows: take loess, add 10 times the amount of water, decoct 3 times, 1 hour each time, concentrate, let stand, take the supernatant, filter it through a 300-mesh sieve, and then centrifuge at high speed to make 1L of water extract equivalent to 1kg of loess; the raw material loess is loess from the place of origin of natural dragon bones or dragon teeth.

[0047] Example 1: Imitation Fossil-Based Medicinal Materials

[0048] (1) Mineral phase transformation

[0049] Take 1 kg of bovine bone blocks free of organic matter, cut them into 1-3 cm blocks, place them in a reaction vessel, add 10 L of mineralization solution with pH 10.6 (each L of mineralization solution contains 0.053 kg sodium carbonate, 0.042 kg sodium bicarbonate, 0.008 kg calcium hydroxide, and 500 mL of loess water extract), stir at 3 MPa pressure and 100 rpm / min, keep at 70 ℃ for 7 days, remove the bone blocks, pre-sinter at 300 ℃ for 3 h, and finally sinter at 600 ℃ for 2 h;

[0050] (2) Isostatic pressing

[0051] Take the bone block obtained in step (1), mix it with the bone powder obtained in step (1) at a mass ratio of bone block to bone powder of 1:15, put it into a mold, seal it, and then place it in a cold isostatic press. Press it at 250 MPa for 0.5 h, take out the mold, open the mold, take out the pressed block from the mold, break the pressed block, remove the bone powder part, and you will get the imitation keel.

[0052] Example 2: Imitation Fossil-Based Medicinal Materials

[0053] (1) Mineral phase transformation

[0054] Take 1 kg of sheep bone blocks free of organic matter, cut them into 1-3 cm pieces, place them in a reaction vessel, add 5 L of mineralization solution with a pH of about 9.7 (each L of mixed aqueous solution contains 0.007 kg sodium carbonate, 0.036 kg sodium bicarbonate, 0.001 kg calcium hydroxide, and 200 mL loess water extract), stir at 1 MPa pressure and 100 rpm / min, keep warm at 80 ℃ for 2 days, remove the bone blocks, pre-sinter at 200 ℃ for 4 h, and finally heat treat at 600 ℃ for 1 h.

[0055] (2) Isostatic pressing

[0056] Take the bone block obtained in step (1), add 5 times the amount (g / g) of loess and mix well. Put it into a mold, seal it, and then place it in a cold isostatic press. Press it at 150 MPa for 1 h, let it cool, take out the mold, open the mold, take out the pressed block from the mold, break the pressed block, separate it, and you will get the imitation keel.

[0057] Example 3: Imitation Fossil-Based Medicinal Materials

[0058] (1) Mineral phase transformation

[0059] Take 1 kg of horse bone blocks free of organic matter, cut them into 1-3 cm blocks, place them in a reaction vessel, add 10 L of mineralization solution with pH 10.6 (each L of mixed aqueous solution contains 0.04 kg sodium carbonate, 0.02 kg sodium bicarbonate, 0.001 kg calcium hydroxide, and 300 mL loess water extract), stir at 5 MPa pressure and 100 rpm / min, keep at 60 ℃ for 2 days, remove the bone blocks, pre-sinter at 300 ℃ for 3 h, and finally sinter at 600 ℃ for 2 h;

[0060] (2) Isostatic pressing

[0061] Take the bone block obtained in step (1), mix it with the bone powder obtained in step (1) at a mass ratio of bone block to bone powder of 1:8, put it into a mold, seal it, place it in a cold isostatic press, press it at 200 MPa for 2 h, take out the mold, open the mold, take out the pressed block from the mold, break the pressed block, separate it, and you will get the imitation keel.

[0062] Example 4: Imitation Fossil-Based Medicinal Materials

[0063] (1) Mineral phase transformation

[0064] Take 1 kg of bovine teeth without organic matter, break them into 0.3-1 cm pieces, place them in a reaction vessel, add 3 L of mineralization solution with pH 10.6 (each L of mixed aqueous solution contains 0.053 kg sodium carbonate, 0.042 kg sodium bicarbonate, and 0.008 kg calcium hydroxide), stir at 6 MPa pressure and 200 rpm / min, keep at 80 ℃ for 14 days, remove the teeth, pre-sinter at 300 ℃ for 3 h, and finally sinter at 500 ℃ for 1.5 h and 700 ℃ for 0.5 h.

[0065] (2) Isostatic pressing

[0066] Take the teeth obtained in step (1), mix them with the powder of the teeth obtained in step (1) at a mass ratio of bone block to bone powder of 1:1, put them into a mold, seal it, place it in a hot isostatic press, press it at 200 ℃ and 150 MPa for 1 h, take out the mold, open the mold, take out the pressed block from the mold, break the pressed block, separate it, and you will get the imitation dragon teeth.

[0067] The beneficial effects of the present invention will be illustrated below through experimental examples.

[0068] Experimental Example 1: Verification of the consistency between quality and efficacy of imitation fossil-based Chinese medicinal materials

[0069] 1. Identification of physical characteristics

[0070] Sensory evaluation of the preparation process flowchart of fossil-based medicinal materials Figure 1 Specifically, the properties of the imitation fossil-like medicinal materials prepared in Examples 1-3 were observed, that is, by sight, touch, smell, and taste, describing their shape, surface (color and characteristics), texture, cross-section (fractured or cut surface), odor, and whether they had a sucking sensation on the tongue, while using natural dragon bone as a reference control. The results are shown in Table 1.

[0071] Table 1. Description of traits

[0072]

[0073] The results above show that the imitation keel is similar in appearance to the natural keel, and is hard, white, and highly absorbent.

[0074] 2. Organic matter testing

[0075] Natural dragon bone and imitation fossil medicinal materials were crushed and placed in a ceramic crucible, then heated in a muffle furnace at 250°C. The presence of flammable organic matter such as fat and protein was determined by whether black smoke or a burnt smell was produced. No black smoke or burnt smell indicated the absence of organic matter, while the presence of black smoke or burnt smell indicated the presence of organic matter.

[0076] Lipid detection (Sudan Red staining method): Soak natural dragon bone and imitation fossil medicinal materials in Sudan Red solution for 10 minutes, rinse with water, and observe whether they are stained red; if they are stained red, it is determined that they contain lipids, otherwise it is determined that they do not contain lipids.

[0077] Protein detection (biuret reaction): Grind natural dragon bone and imitation fossil medicinal materials, mix with NaOH, heat to boiling, add CuSO4 solution dropwise, and observe whether it turns blue; turning blue indicates the presence of protein and polypeptide components, otherwise it indicates the absence of protein and polypeptide components.

[0078] The results of organic matter testing are shown in Table 2.

[0079] Table 2 Organic Matter Detection Results

[0080]

[0081] The above results indicate that the prepared imitation fossil-like medicinal materials are inorganic, just like natural dragon bones.

[0082] 3. Density detection

[0083] Natural dragon bone and imitation fossil medicinal materials were collected and their true density was tested according to the method in the national standard (GB / T23561.2-2009); the bulk density was tested according to the method 0993, General Chapter 1 of the 2020 edition of the Chinese Pharmacopoeia. The results are shown in Table 3.

[0084] Table 3 Density Detection Results

[0085]

[0086] Experimental results show that the density of the prepared imitation fossil medicinal material is similar to that of natural dragon bone.

[0087] 4. Phase composition and crystallinity analysis

[0088] Natural dragon bone and imitation fossil-like medicinal materials were crushed, ground, and passed through a No. 9 sieve to prepare test samples. X-ray diffraction was used for identification. Measurement conditions: copper target X-ray tube, tube voltage 40 kV, tube current 40 mA; scanning range: 2θ angle 10–80°; detector: solid-state counter (XOL-D); DS (diverging slit) and SS (anti-scattering slit) 2.0 mm, RS (receiving slit) 0.1 mm; step size 0.03° / step; scanning speed 0.4 s / step. X-ray diffraction characteristic patterns were obtained, and crystallinity was calculated by matching with a database.

[0089] (1) X-ray characteristic spectrum is shown in Figures 2-3 ;

[0090] (2) Crystallinity is shown in Table 4;

[0091] Table 4 Crystallinity Test Results

[0092]

[0093] The results above show that the imitation fossil-based medicinal materials are similar to natural dragon bones in terms of the position of characteristic peaks and crystallinity.

[0094] 5. Mass ratio of calcium to phosphorus

[0095] Natural dragon bone and imitation fossil medicinal materials were collected, and the calcium and phosphorus content was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) according to General Chapter 0411 of Part IV of the 2020 Chinese Pharmacopoeia, and the mass ratio was calculated.

[0096] Table 5. Results of Calcium-Phosphorus Mass Ratio Test

[0097]

[0098] The results above show that the calcium-to-phosphorus ratio of the imitation fossil-like medicinal material, dragon bone, is similar to that of natural dragon bone, both being greater than 2.2.

[0099] 6. Pharmacodynamic evaluation based on sedative and tranquilizing effects

[0100] 6.1 Animal Insomnia Model

[0101] A multi-platform aquatic environment method was used. Mice were randomly placed on platforms approximately 3 cm in diameter within an experimental chamber, with each platform spaced 3 cm apart. Tap water was poured into the chamber, with the water level approximately 1 cm above the platform surface. Mice were deprived of sleep for 12 hours daily (from 9:00 AM to 9:00 PM the following day) for 21 days, and spent the remaining time resting in the incubator. After modeling, the mice exhibited photophobia, reduced activity, and lethargy, indicating successful modeling.

[0102] 6.2 Investigational Drug

[0103] Natural dragon bone decoction: Take 100 g of dragon bone and add 1 L of water, decoct for 1 hour, take the filtrate, add another 1 L, decoct for 0.5 hours, combine the filtrates, and concentrate to 200 mL.

[0104] Imitation dragon bone decoction: Take 100 g of imitation dragon bone (prepared according to Example 1), add 1 L of water, decoct for 1 h, take the filtrate, add another 1 L, decoct for 0.5 h, combine the filtrates, and concentrate to 200 mL.

[0105] 6.3 Dosing and Grouping

[0106] Healthy mice were used as the control group, and insomniac mice were randomly divided into a model group, a natural dragon bone decoction group, and a modified dragon bone decoction group; 10 mice in each group. Drug administration began one week after model establishment. The control group and model group were administered the same dose of distilled water by gavage once daily for 14 consecutive days. The dragon bone decoction group and Example 1 group received 4.505 g / kg of the decoction, administered by gavage once daily for 14 consecutive days.

[0107] 6.4 Detection Methods

[0108] Open field test: Mice were introduced from a fixed location into a 50 cm × 50 cm open field and allowed to move freely. The movement of each group of mice was recorded over 5 minutes. After each test, before introducing another mouse, feces and urine stains on the field were cleaned up and wiped with alcohol.

[0109] MDA content determination: Accurately weigh brain tissue, add physiological saline at a ratio of weight (g):volume (mL) = 1:9, then add grinding beads for mechanical homogenization, centrifuge at 12000 g for 10 min, collect the supernatant, and finally dilute with physiological saline to the appropriate concentration. Determine the tissue protein concentration using the BCA method, following the instructions of the MDA content kit.

[0110] 6.5 Results

[0111] 6.5.1 Total distance traveled within the open field

[0112] See results Figure 4 ,from Figure 4 It can be seen that compared with the blank group, the amount of movement of mice in the model group was significantly reduced (p<0.05). After continuous administration of dragon bone decoction and imitation fossil medicinal decoction, the total distance moved in the open field was significantly increased compared with the model group (p<0.05).

[0113] 6.5.2 MDA content in brain tissue

[0114] See results Figure 5 ,from Figure 5 It can be seen that, compared with the blank group, the MDA content in the brain tissue of mice in the model group was significantly increased (p<0.001). After continuous administration of the decoction of dragon bone and the decoction of imitation fossil medicinal materials, the MDA content was significantly reduced compared with the model group (p<0.05), indicating that dragon bone and imitation fossil medicinal materials can improve the pathological damage caused by insomnia.

[0115] The above results indicate that the prepared imitation dragon bone has the same sedative and tranquilizing effects as natural dragon bone.

[0116] In summary, this invention, based on the principle of natural mineralization of biological bones, uses mineral phase transformation and isostatic pressing densification techniques to obtain imitation fossil-like medicinal materials with the same structure as natural fossil-like medicinal materials. Animal experiments have verified that natural dragon bone and the imitation fossil-like medicinal materials of this invention have comparable calming and sedative effects, and thus possess practical application value.

Claims

1. A method for preparing a fossil-like medicinal material, characterized in that: It includes the following steps: The mineralized tissue of mammals, which contains no organic matter, is subjected to mineral phase transformation and isostatic pressing to achieve compaction. Among them, the mineral phase transformation involves cutting or crushing the mineralized tissue that does not contain organic matter, placing it in a mineralizing liquid, stirring under high pressure and heat, and then sintering and solidifying it. Isostatic compaction involves pressing the mineralized structure after the mineral phase transformation in an isostatic press. The mineralizing solution is a mixed solution with a pH of 9-11, composed of sodium carbonate, sodium bicarbonate, calcium hydroxide, and loess water extract. The loess mentioned is loess from the original habitat of natural dragon bones or dragon teeth.

2. The preparation method according to claim 1, characterized in that: The mineral phase transformation includes the following steps: Take a mineralized tissue without organic matter, cut or crush it, place it in a mineralization liquid, and keep it heated and stirred for 2 to 14 days under the conditions of pressure 1-10 MPa, rotation speed 100-200 rpm / min and temperature 50-80℃. Take out the mineralized tissue, pre-sinter it, and then sinter it to obtain the product. The pieces are cut or crushed into 0.3-3 cm pieces.

3. The preparation method according to claim 2, characterized in that: The mass-to-volume ratio of the mineralized tissue to the mineralized liquid is 1 kg : 3~10 L; the pre-sintering temperature is 200~300 ℃, and the time is 3~4 h; the sintering is performed 1~2 times, with each time the temperature is 500~700 ℃ and the time is 0.5~2 h; Each liter of the loess water extract is equivalent to 0.5~1.5 kg of loess.

4. The preparation method according to claim 1, characterized in that: The isostatic compaction process includes the following steps: Take mineralized tissue blocks that have undergone mineral phase transformation, add bone meal or loess and mix well, then fill them into molds and press them in an isostatic press. The bone powder is a powder of mineralized tissue that has undergone mineral phase transformation.

5. The preparation method according to claim 4, characterized in that: The bone meal or loess is 1-15 times the amount of the mineralized tissue; The isostatic press is a cold isostatic press and / or a hot isostatic press; The pressure of the cold isostatic press is 100-500 MPa, and the pressing time is 0.2-2 h; The hot isostatic press has a pressure of 100-250 MPa, a temperature of 100-300°C, and a pressing time of 0.2-2 h.

6. The preparation method according to claim 1, characterized in that: The mammals include cattle, horses, deer, donkeys, sheep, and pigs; the mineralized tissues are bones, teeth, and horns.

7. A type of fossil-like medicinal material, characterized in that: It is prepared by the preparation method described in any one of claims 1 to 6.

8. The fossil-like medicinal material according to claim 7, characterized in that: The imitation fossil medicinal materials include imitation dragon bones and imitation dragon teeth; The mass percentage of calcium and phosphorus in the imitation keel and imitation keel teeth shall not be less than 2.2%.

9. The use of the fossil-like medicinal material as described in claim 7 or 8 in the preparation of sedative and tranquilizing drugs.

Images