Plant growth regulator composition for regulating growth of cotton as well as preparation method and application of plant growth regulator composition

Abstract

The invention discloses a composition containing a plant growth regulator for regulating cotton growth and a preparation method and application thereof, and relates to the field of agricultural growth regulators, the effective components of the composition comprise prohexadione calcium, thidiazuron and mepiquat chloride, and the weight ratio of prohexadione calcium to thidiazuron to mepiquat chloride is (0.1-1): (0.001-10): (0.1-300). The composition disclosed by the invention can be applied to a cotton field, is used for regulating the growth of cotton, and can control the plant height of the cotton, shape an ideal plant type and promote the yield increase of the cotton.

Description

Technical Field

[0001] This invention relates to the field of agricultural growth regulators, and more particularly to a plant growth regulator composition containing a plant growth regulator for regulating cotton growth, its preparation method, and its application. Background Technology

[0002] Cotton is an important field crop and a major raw material for the textile industry in China, occupying a vital position in the national economy. Cotton possesses indeterminate growth characteristics and high plasticity. Utilizing plant growth regulators to shape ideal plant shapes and high-light-efficiency canopy structures, reduce boll shedding, and increase yield are widely used techniques in cotton production. Practice has shown that the effects of plant growth regulators vary at different stages of crop growth and for different crops. Furthermore, different combinations of plant growth regulators produce significantly different effects. Therefore, researching combinations of regulators is of great significance for solving plant growth problems.

[0003] Calcium cyclohexane, a novel plant growth regulator belonging to the cyclohexanecarboxylic acid class, was jointly developed by Japan's Combined Chemical Industry Co., Ltd. and BASF of Germany. It is a late-stage inhibitor of gibberellin biosynthesis. Its main functions include: inhibiting vegetative growth, promoting reproductive growth, promoting lateral bud growth and root development, and maintaining dark green stems and leaves; controlling flowering time, promoting flower bud differentiation, and increasing fruit set rate; promoting the accumulation of sugar and dry matter, promoting fruit color change, and improving storage resistance; shortening internodes and reducing lodging; reducing herbicide damage; and improving plant resistance to cold, drought, and disease, ultimately achieving increased yield and improved quality.

[0004] Thiabenone, a urea-based plant growth regulator, is used as a defoliant for cotton at high concentrations, and as a highly effective cytokinin at low concentrations. It can promote cell division, delay leaf senescence, and enhance plant stress resistance and disease resistance.

[0005] Mepiquat chloride has a retardant effect on the vegetative growth of plants. It can be absorbed through the leaves and roots and translocated throughout the plant, reducing the activity of gibberellins in the plant, thereby inhibiting cell elongation, weakening the growth of terminal buds, controlling the longitudinal and lateral growth of the plant, shortening internodes, resulting in a compact plant shape, darker leaf color, and reduced leaf area. It also enhances chlorophyll synthesis, preventing excessive vegetative growth and delaying canopy closure. In addition, mepiquat chloride can improve cell membrane stability and increase the plant's resistance to adverse conditions.

[0006] Although each plant growth regulator has a specific purpose, the application techniques are quite strict, and it can only produce specific effects on the target plant under specific application conditions (including external factors). For achieving the ideal plant shape in cotton, combinations of growth regulators that promote topping, increase yield, and improve cotton quality remain extremely valuable. Summary of the Invention

[0007] Purpose of the invention

[0008] To overcome the above shortcomings, the present invention aims to provide a plant growth regulator composition for regulating cotton growth, its preparation method and application. The composition of the present invention can be applied in cotton fields to regulate cotton growth, control cotton plant height, shape ideal plant shape and promote cotton yield.

[0009] Solution

[0010] To achieve the objectives of this invention, the technical solution adopted is as follows:

[0011] In a first aspect, the present invention provides a plant growth regulator composition for regulating cotton growth, the active ingredients of which include calcium cyclohexane, thiamethoxam, and mepiquat chloride, wherein the weight ratio of calcium cyclohexane, thiamethoxam, and mepiquat chloride is (0.1-1):

[0012] (0.001-10): (0.1-300).

[0013] Further, the weight ratio of the calcium cyclohexane, thiamethoxam, and mepiquat chloride is (0.2-1):(0.0025-0.0066):

[0014] (0.25-6.25)((6~30):(0.075~0.2):(7.5~187.5)), optionally 1:

[0015] (0.0025~0.033):(0.25~31.25), preferably 1:(0.001~1):(0.25~31.25), preferably 1:(0.0025~0.034):(0.25~10.5), preferably 1:(0.0025~0.1):(0.25~12.5), preferably 1:(0.0025~0.5):(0.25~10.42), preferably 1:(0.0025~0.033):(0.25~10.42), preferably 1:(0.0025~0.1):(0.25~10.42), preferably 1:(0.0025~0.05):

[0016] (0.25~10.42), preferably (0.0025~0.012): (0.25~10.42), preferably 1:

[0017] (0.0025~0.01):(0.25~6.25), preferably 1:(0.0025~0.0067):(0.25~6.25), preferably 18:0.15:11.76, 18:0.15:8.85, 18:0.15:44.1 or 18:0.15:176.4.

[0018] Further, the active ingredient accounts for 1% to 99% of the total weight of the composition, optionally 5% to 80%, optionally 5% to 75%, optionally 8% to 60%.

[0019] Alternatively, the dosage of calcium cyclohexane can be 6–30 g ai / ha.

[0020] Alternatively, the dosage of thiamethoxam is 0.075–0.2 g ai / ha.

[0021] Alternatively, the dosage of mepiquat chloride is 7.5–187.5 g ai / ha.

[0022] Furthermore, the formulation of the plant growth regulator composition is one or more of the following: wettable powder, water-dispersible granules, dry suspension, water suspension, dispersible oil suspension, and granules.

[0023] Optionally, the plant growth regulator composition further includes adjuvants, which are selected from one or more of the following: wetting agents, dispersants, fillers, emulsifiers, thickeners, dispersion media, disintegrants, solvents, preservatives, antifreeze agents, specific gravity regulators, defoamers, oil-phase wall materials, aqueous-phase wall materials, and water.

[0024] Furthermore, the formulation of the plant growth regulator composition is a wettable powder;

[0025] Optionally, the plant growth regulator composition comprises the following raw material components in weight percentage:

[0026] The effective ingredient content is 5-75%, optionally 9%-60%;

[0027] Wetting agent 1-5%, optionally 2-4%;

[0028] Dispersant 2-15%, optionally 4-10%, optionally 5-8%;

[0029] The filler content is 20-85%, optionally 30-85%, optionally 31-81%, optionally supplemented to 100%.

[0030] Preferably, the plant growth regulator composition comprises the following components by weight percentage: 9-60% active ingredient, 2-4% wetting agent, 5-8% dispersant, and the remainder being filler.

[0031] Preferably, the plant growth regulator composition comprises the following components in weight percentage: 6-36% calcium cyclohexane, 0.05-0.3% thiamethoxam, 2.95-23.52% mepiquat chloride, 2-4% wetting agent, 5-8% dispersant, and filler balance.

[0032] Optionally, the wetting agent is one or more of the following: dodecyl naphthalene sulfonate, fatty alcohol polyoxyethylene ether, calcium dodecylbenzene sulfonate, sodium dodecyl sulfate, phenol sulfonate, sodium diisobutylnaphthalene sulfonate, polyoxyethylene ether phosphate, etc.

[0033] Optionally, the dispersant includes one or more of alkyl naphthalene sulfonate formaldehyde condensate, lignin sulfonate, polycarboxylate, alkyl naphthalene sulfonate, etc.

[0034] Optionally, the filler includes one or more of silica, ammonium sulfate, and kaolin; or, alternatively, the filler includes at least two of silica, ammonium sulfate, and kaolin.

[0035] Furthermore, the formulation of the plant growth regulator composition is a water-dispersible granule;

[0036] Optionally, the plant growth regulator composition comprises the following raw material components in weight percentage:

[0037] The active ingredient content is 10-70%, optionally 12%-67%;

[0038] Wetting agent 0-10%, optionally 0-4%;

[0039] The dispersant is 1-15%, optionally 2-10%, optionally 4-10%, optionally 6-10%;

[0040] The filler content is 10-80%, optionally 21.65-81.55%, and optionally up to 100%.

[0041] Optionally, the plant growth regulator composition comprises the following components by weight percentage: 12-67% active ingredient, 0-4% wetting agent, 2-10% dispersant, and the remainder being filler.

[0042] Preferably, the plant growth regulator composition comprises the following components in weight percentage: 3.6-7.5% calcium cyclohexane, 0.03-0.05% thiamethoxam, 8.82-58.8% mepiquat chloride, 0-4% wetting agent, 2-10% dispersant, and the balance of filler.

[0043] Optionally, the wetting agent is one or more of the following: dodecyl naphthalene sulfonate, fatty alcohol polyoxyethylene ether, calcium dodecylbenzene sulfonate, sodium dodecyl sulfate, phenol sulfonate, sodium diisobutylnaphthalene sulfonate, polyoxyethylene ether phosphate, etc.

[0044] Optionally, the dispersant includes one or more of alkyl naphthalene sulfonate formaldehyde condensate, lignin sulfonate, polycarboxylate, alkyl naphthalene sulfonate, etc.

[0045] Optionally, the filler includes one or more of corn starch, ammonium sulfate, and kaolin; alternatively, the filler includes at least two of silica, ammonium sulfate, and kaolin.

[0046] Secondly, the present invention provides a method for preparing the plant growth regulator composition described above, wherein calcium cyclohexane, thiamethoxam, mepiquat chloride, wetting agent, dispersant and filler are mixed evenly, and then the mixture is pulverized by air jet milling until the average particle size D50 is less than 5 micrometers, and then mixed evenly again to obtain the final product.

[0047] Thirdly, the present invention provides a method for preparing the plant growth regulator composition described above, characterized in that calcium cyclohexane, thiamethoxam, mepiquat chloride, wetting agent, dispersant and filler are mixed evenly, then pulverized by air jet milling until the average particle size D50 is less than 5 micrometers, water is added and kneaded, extruded and granulated, dried and sieved to obtain the product;

[0048] Optionally, add 10% water and knead for 20-50 minutes.

[0049] Fourthly, the present invention provides the application of the plant growth regulator composition described in the first aspect, or the preparation prepared by the preparation method described in the second or third aspect, in regulating cotton growth, topping, and increasing yield.

[0050] Fourthly, the present invention provides a method of using the plant growth regulator composition described in the first aspect, or the preparation of the formulation prepared by the preparation method described in the second or third aspect, characterized in that the plant growth regulator composition is applied during the growth period of cotton, and optionally applied once or multiple times at any time during the cotton budding stage, initial flowering stage, or boll-forming stage of the cotton growth period.

[0051] Optionally, the dosage of calcium cyclohexane is 2–120 g ai / ha, preferably 3–60 g ai / ha, and more preferably 6–30 g ai / ha;

[0052] Optionally, the dosage of thiamethoxam is 0.025–0.8 g ai / ha, preferably 0.0375–0.4 g ai / ha, and more preferably 0.075–0.2 g ai / ha;

[0053] Optionally, the dosage of mepiquat chloride is 2.5–750 g ai / ha, preferably 3.75–375 g ai / ha, and more preferably 7.5–187.5 g ai / ha.

[0054] Beneficial effects

[0055] (1) The composition of the present invention can be applied in cotton fields to regulate cotton growth, control cotton plant height, shape ideal plant shape, promote cotton yield, and has the uses of cotton shaping, topping, and yield increase.

[0056] (2) The plant growth regulator composition of the present invention can be sprayed onto the stems and leaves of cotton plants. When using this plant growth regulator composition, in addition to using the active ingredient directly, the composition containing the target active ingredient can be formulated, or the active ingredients can be formulated separately and then mixed. During application, each active ingredient of this application can be applied separately or simultaneously.

[0057] (3) The composition of the present invention can be applied once or multiple times at different growth stages of cotton. Detailed Implementation

[0058] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0059] Furthermore, to better illustrate the present invention, numerous specific details are provided in the following detailed embodiments. Those skilled in the art should understand that the present invention can be practiced without certain specific details. In some embodiments, materials, methods, means, etc., well-known to those skilled in the art, are not described in detail in order to highlight the spirit of the present invention.

[0060] Unless otherwise expressly stated, throughout the specification and claims, the term "comprising" or its variations such as "including" or "comprises" shall be understood to include the stated elements or components without excluding other elements or other components.

[0061] Unless otherwise specified, all percentages below are by weight.

[0062] In the following examples, thiabendazole, calcium cyclohexane, and mepiquat chloride are all commercially available, as are other commercially available products.

[0063] Among them, 98% thiamethoxam TC and 98% calcium cyclohexane TC came from Max (Rudong) Chemical Co., Ltd.; 98% mepiquat chloride TC was purchased from Jiangsu Runze Agrochemical Co., Ltd.

[0064] The present invention will be further described in detail below through some embodiments.

[0065] Test Example 1: Evaluation of the Combined Effect of Indoor Compositions

[0066] Technical grade drug: Accurately weigh a certain mass of technical grade drug, dissolve it in DMF (dimethylformamide), add water containing 0.1% Tween 80 emulsifier, stir evenly, and prepare a solution of a certain concentration for later use.

[0067] Preparation: Weigh directly, dilute with water, and then process.

[0068] Experimental method: Refer to the stem and leaf spray method of "Indoor Bioassay Test Guidelines for Pesticides.NY / T 2061.2-2011 Plant Growth Regulators Part 2: Test for Promoting Uniform Plant Growth".

[0069] Cotton was used as the experimental material. Three active ingredients—calcium cyclamate (code: A), thiamethoxam (code: B), and mepiquat chloride (code: C)—were mixed in different proportions. Cotton plants at the peak budding stage with similar growth were selected for application, with 10 plants per treatment. Ten days after application, the lengths of the first fruiting branch and the longest vegetative branch were measured. The inhibition rates of the fruiting branch and the vegetative branch were calculated based on the experimental data.

[0070] The Gowing method was used to test the growth-regulating effect on cotton after blending, namely:

[0071] E0 = X + Y - XY / 100

[0072] In the formula, X represents the regulatory effect of binary plant growth regulators A+B, A+C, and B+C at a dosage of P; Y represents the regulatory effect of single plant growth regulators A, B, and C at a dosage of Q; E0 represents the theoretical regulatory effect of plant growth regulator (A+B+C) at a dosage of (P+Q); and E represents the actual regulatory effect of each treatment. When E-E0>10%, it indicates that the mixed plant growth regulators have a synergistic effect; when E-E0<-10%, it indicates that the mixed plant growth regulators have an antagonistic effect; and when the E-E0 value is between ±10%, it indicates that the mixed plant growth regulators have an additive effect.

[0073] The effects of different ratios of the composition on cotton growth regulation are shown in Table 1.

[0074] Table 1. Effects of different ratios of the composition on cotton growth regulation.

[0075]

[0076] The inhibition rates of the first fruit branch and the oil-fritters were evaluated using the Gowing formula, and the results are shown in Tables 2 and 3. In Tables 1-3, A represents calcium cyclohexane; B represents thiamethoxam; C represents mepiquat chloride; A1, A2, and A3 represent different concentrations of calcium cyclohexane; B1, B2, B3, and B4 represent different concentrations of thiamethoxam; and C1, C2, C3, and C4 represent different concentrations of mepiquat chloride.

[0077] Table 2 Evaluation results of the effects of different formulations on cotton fruiting branch regulation and combined effects.

[0078]

[0079] Table 2 shows that calcium cyclohexane, thiamethoxam, and mepiquat chloride all exhibit synergistic effects in inhibiting the first fruiting branch of cotton at a weight ratio of (6–30):(0.075–0.2):(7.5–187.5), especially at 1:(0.0025–0.034):(0.25–31.25), preferably at 1:(0.0025–0.033):(0.25–31.25), preferably at 1:(0.0025–0.034):(0.25–10.5), preferably at 1:(0.0025–0.033). The following formulas show good synergistic effects: (0.25~10.42), preferably in 1: (0.0025~0.012): (0.25~10.42), preferably in 1: (0.0025~0.011): (0.25~10.42), preferably in 1: (0.0025~0.011): (0.25~6.25), preferably in 1: (0.0025~0.0067): (0.25~6.25), preferably in 1: (0.0025~0.0067): (1.25~6.25).

[0080] Table 3. Evaluation results of the conditioning effect and combined effect of different ratios of the composition on cotton strips.

[0081]

[0082] Table 2 shows that calcium cyclohexane, thiabendazole, and mepiquat chloride all exhibit synergistic effects on the inhibition of cottonseed oil slicks at a weight ratio of (6–30):(0.075–0.2):(7.5–187.5), especially at 1:(0.0025–0.034):(0.25–31.25), preferably at 1:(0.0025–0.033):(0.25–31.25), preferably at 1:(0.0025–0.034):(0.25–10.5), preferably at 1:(0.0025–0.033): The ratios (0.25~10.42), preferably 1:(0.0025~0.012):(0.25~10.42), preferably 1:(0.0025~0.011):(0.25~10.42), preferably 1:(0.0025~0.011):(0.25~6.25), preferably 1:(0.0025~0.0067):(0.25~6.25), and preferably 1:(0.0025~0.0067):(1.25~6.25) all show good synergistic effects.

[0083] Optionally, the weight ratio of thiabendazole and mepiquat chloride is 1:(37.5-1359), or alternatively 1:(37.5-937.5).

[0084] Test Example 2

[0085] The composition formulations used in this test example include those of Examples 1-4, and comparative examples 1-8 were also provided.

[0086] In the following examples and comparative examples, unless otherwise stated, all percentages are mass percentages.

[0087] Example 1: The mass ratio of calcium cyclohexane: thiamethoxam: mepiquat chloride was 18:0.15:11.76;

[0088] Components composition% Remark Cyclocycline calcium 36 Active ingredients thiamethoxam 0.3 Active ingredients Mepiquat 23.52 Active ingredients Morwet EFW alkyl naphthalene sulfonate 4 Wetting agent, purchased from Noryon GEROPON T-36 polycarboxylate 5 Dispersant, purchased from Solvay precipitate 3 filler Kaolin 28.18 (to make up 100) filler

[0089] The preparation method of the above-mentioned plant growth regulator composition includes the following steps:

[0090] Weigh out the active ingredients calcium cyclohexane, thidiazuron, mepiquat chloride, wetting agent, dispersant and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Mix them evenly again to obtain a 59.82% wettable powder of calcium cyclohexane, thidiazuron and mepiquat chloride.

[0091] Example 2: The mass ratio of calcium cyclohexane: thiamethoxam: mepiquat chloride was 18:0.15:8.85;

[0092] Components composition% Remark Cyclocycline calcium 6 Active ingredients thiamethoxam 0.05 Active ingredients Mepiquat 2.95 Active ingredients Sodium dodecyl sulfate 2 wetting agent Borresperse NA sodium lignosulfonate 8 Dispersant, purchased from Borregaard ammonium sulfate 12 filler Kaolin 69 (to make 100) filler

[0093] The preparation method of the above-mentioned plant growth regulator composition includes the following steps:

[0094] Weigh out the active ingredients calcium cyclohexane, thiabendazole, mepiquat chloride, wetting agent, dispersant and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Mix them evenly again to obtain 9% wettable powder of calcium cyclohexane, thiabendazole and mepiquat chloride.

[0095] Example 3: The mass ratio of calcium cyclohexane: thiamethoxam: mepiquat chloride was 18:0.15:44.1;

[0096]

[0097]

[0098] The preparation method of the above-mentioned plant growth regulator includes the following steps:

[0099] Weigh the active ingredients calcium cyclohexane, thiabendazole, mepiquat chloride, wetting agent, dispersant, and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Add 10% water to the resulting mixture in a kneader and knead for 30 minutes. Granulate the resulting kneaded mixture through an extrusion granulator, and then dry it at 60 degrees Celsius and sieve it to obtain the plant growth regulator calcium cyclohexane·thiabendazole·mepiquat chloride 12.45% water-dispersible granules.

[0100] Example 4: The mass ratio of calcium cyclohexane: thiamethoxam: mepiquat chloride was 22.5:0.15:176.4.

[0101] Components composition% Remark Cyclocycline calcium 7.5 Active ingredients thiamethoxam 0.05 Active ingredients Mepiquat 58.8 Active ingredients Sodium dioctyl succinate sulfonate 2 wetting agent Ufoxane 3A Sodium Lignosulfonate 10 Dispersant, purchased from Borregaard corn starch 6 filler Kaolin 15.65 (make up to 100) filler

[0102] The preparation method of the above-mentioned plant growth regulator includes the following steps:

[0103] Weigh out the active ingredients calcium cyclohexane, thiabendazole, mepiquat chloride, wetting agent, dispersant, and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is below 5 micrometers. Add 10% water to the resulting mixture in a kneader and knead for 30 minutes. Granulate the resulting kneaded mixture through an extrusion granulator, and then dry it at 60 degrees Celsius and sieve it to obtain the plant growth regulator calcium cyclohexane·thiabendazole·mepiquat chloride 66.35% water-dispersible granules.

[0104] Comparative Example 1, 15% calcium cyclohexane suspension, was produced by Zhejiang Shijia Technology Co., Ltd.

[0105] Comparative Example 2, 0.1% thiamethoxam soluble concentrate, was produced by Xianyang Defeng Co., Ltd.

[0106] Comparative Example 3, 98% mepiquat chloride soluble powder, was produced by Nantong Jinling Agricultural Chemical Co., Ltd.

[0107] Comparative Example 4: The mass ratio of calcium cyclohexane to thiabendazole was 18:0.15;

[0108] Components composition% Remark Cyclocycline calcium 36 Active ingredients thiamethoxam 0.3 Active ingredients Morwet EFW alkyl naphthalene sulfonate 4 Wetting agent, purchased from Noryon GEROPON T-36 polycarboxylate 5 Dispersant, purchased from Solvay precipitate 3 filler Kaolin 51.7 (make up to 100) filler

[0109] The preparation method of the above-mentioned plant growth regulator composition includes the following steps:

[0110] Weigh out the active ingredients calcium cyclohexane, thidiazuron, wetting agent, dispersant and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Mix them evenly again to obtain calcium cyclohexane·thidiazuron 36.3% wettable powder.

[0111] Comparative Example 5: The mass ratio of calcium cyclohexane to mepiquat chloride was 18:11.76;

[0112] Components composition% Remark Cyclocycline calcium 36 Active ingredients Mepiquat 23.52 Active ingredients Morwet EFW alkyl naphthalene sulfonate 4 Wetting agent, purchased from Noryon GEROPON T-36 polycarboxylate 5 Dispersant, purchased from Solvay precipitate 3 filler Kaolin 28.48 (make up to 100) filler

[0113] The preparation method of the above-mentioned plant growth regulator composition includes the following steps:

[0114] Weigh out the active ingredients calcium cyclohexane, mepiquat chloride, wetting agent, dispersant and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Mix them evenly again to obtain 59.52% wettable powder of calcium cyclohexane and mepiquat chloride.

[0115] Comparative Example 6: The mass ratio of thiabendazole to mepiquat chloride was 0.15:11.76;

[0116]

[0117]

[0118] The preparation method of the above-mentioned plant growth regulator composition includes the following steps:

[0119] Weigh out the active ingredients thiabendazole, mepiquat chloride, wetting agent, dispersant and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Mix them evenly again to obtain 23.82% thiabendazole·mepiquat chloride wettable powder.

[0120] Comparative Example 7: The mass ratio of calcium cyclohexane: thiamethoxam: mepiquat chloride was 3 + 0.075 + 23.93.

[0121] Components composition% Remark Cyclocycline calcium 3 Active ingredients thiamethoxam 0.075 Active ingredients Mepiquat 23.93 Active ingredients Morwet D-425 Alkyl Naphthalene Sulfonate Formaldehyde Condensate 4 Dispersant, purchased from Noryon Ufoxane 3A Sodium Lignosulfonate 2 Dispersant, purchased from Paulig. ammonium sulfate 10 filler Kaolin 56.995 (padded to 100) filler

[0122] The preparation method of the above-mentioned plant growth regulator includes the following steps:

[0123] Weigh the active ingredients calcium cyclohexane, thiabendazole, mepiquat chloride, wetting agent, dispersant, and filler according to the above formula, mix them evenly, and then pulverize them through an air jet milling system until the average particle size D50 is less than 5 micrometers. Add 10% water to the resulting mixture in a kneader and knead for 30 minutes. Granulate the resulting kneaded mixture through an extrusion granulator, and then dry it at 60 degrees Celsius and sieve it to obtain the plant growth regulator composition calcium cyclohexane·thiabendazole·mepiquat chloride 27.005% water-dispersible granules.

[0124] Comparative Example 8: The mass ratio of calcium cyclohexane to thiabendazole was 18:0.216;

[0125] Components composition% Remark Cyclocycline calcium 36 Active ingredients thiamethoxam 0.432 Active ingredients Sodium dioctyl succinate sulfonate 2 wetting agent Ufoxane 3A Sodium Lignosulfonate 10 Dispersant, purchased from Borregaard corn starch 6 filler Kaolin 45.568 (padded to 100) filler

[0126] The preparation method of the above-mentioned plant growth regulator includes the following steps:

[0127] Weigh out the active ingredients calcium cyclohexane, thiabendazole, wetting agent, dispersant and filler according to the above formula, mix them evenly, and then pulverize them with an air jet milling system until the average particle size D50 is less than 5 micrometers. Add 10% water to the resulting mixture in a kneader and knead for 30 minutes. Granulate the resulting kneaded mixture by an extrusion granulator, and then dry it at 60 degrees and sieve it to obtain the plant growth regulator composition calcium cyclohexane·thiabendazole 36.432% water dispersible granules.

[0128] field trials

[0129] To clarify the field application effect of the composition, a field efficacy test of the composition in cotton fields was conducted in accordance with the guidelines for pesticide field efficacy tests.

[0130] Test Example 2.1: Cotton Field Plot Experiment

[0131] The experiment was conducted in the 12th Company of the 130th Regiment of the 8th Division of Xinjiang, where cotton growth was generally uniform. Foliar treatment was applied during the peak budding stage of cotton, with a water concentration of 40 L / mu (approximately 0.067 hectares) per treatment, and three replicates were performed. Ten days after application, cotton plant height, the length of the first fruiting branch, and the length of the longest vegetative branch were measured. Yield was determined before harvest in the experimental area, with three randomly selected plots of uniform growth for each treatment, each plot measuring 6.67 m². 2 The number of plants and total number of bolls per unit area were investigated. The weight of 100 cotton bolls was measured, the average weight per boll was calculated, and then the yield per mu (unit of land area) was calculated. Data were analyzed using DPS 7.05 software and Duncan's new multiple range test (DMRT method, p = 0.05).

[0132] The results are shown in Tables 4 and 5. The results indicate that the combination of calcium cyclohexane-thiamethoxam-mepiride significantly outperformed the individual single-agent and binary compositions in inhibiting cotton plant height, fruiting branches, and oil stalks; and significantly outperformed the individual single-agent and binary compositions in promoting cotton boll number and yield.

[0133] Table 4 Results of the test on the inhibitory effect of the composition on cotton growth

[0134]

[0135] Note: Different letters in the subheadings in Table 4 represent significant differences between groups (P < 0.05), while the same letter represents no significant difference.

[0136] Table 5. Results of the experiment on the effect of the composition on cotton yield and its composition.

[0137]

[0138] Note: In Table 5, different letters on the shoulder labels represent significant differences between groups (P < 0.05), while the same letter represents no significant difference.

[0139] Test Example 2.2, Flower Field Area Experiment 1

[0140] The experiment was conducted in Manas County, Shihezi City, Xinjiang, where cotton growth was generally uniform. A foliar spray treatment was applied once during the peak budding stage of the cotton plants, with a water concentration of 40 L / mu (approximately 0.067 hectares), applied to 10 mu (approximately 6.7 hectares) per treatment. Ten days after the spraying, plant height, the length of the first fruiting branch, and the length of the longest vegetative branch were measured. Yield was determined before harvest in the experimental area. Three plots with uniform growth were randomly selected from each treatment, with each plot measuring 6.67 m². 2 The number of plants and total number of bolls per unit area were investigated. 100 cotton bolls were weighed to calculate the average weight per boll and then the yield per mu was calculated. The data were analyzed using DPS7.05 software and Duncan's new multiple range test (DMRT method, p=0.05).

[0141] The results are shown in Tables 6 and 7. The results indicate that the calcium cyclohexane-thiafenuron-mepiride composition, at a dosage ratio of 18 + 0.15 + 8.85 g ai. / ha (total 27 g ai / ha), significantly inhibited cotton plant height, fruiting branches, and oil stalks compared to Comparative Examples 1–3 and Comparative Example 7. Its promoting effect on cotton boll number and yield was also significantly better than that of the single-agent treatments in Comparative Examples 1–3 and Comparative Example 7. The calcium cyclohexane-thiafenuron-mepiride composition, at the same dosage of 27 g ai / ha, was superior to each single agent and other combinations.

[0142] Table 6. Results of cotton growth regulation experiments using the composition in cotton fields

[0143]

[0144]

[0145] Note: Different letters in the subheadings in Table 6 represent significant differences between groups (P < 0.05), while the same letter represents no significant difference.

[0146] Table 7. Results of the experiment on the effect of the composition on cotton yield in cotton fields.

[0147]

[0148] Note: Different letters in the subscripts in Table 7 represent significant differences between groups (P < 0.05), while the same letter represents no significant difference.

[0149] Test Example 2.3: Large-scale cotton field trial

[0150] The experiment was conducted in Shawan County, Shihezi City, Xinjiang, where cotton growth was generally uniform. Foliar spraying was applied 1-2 times during the cotton's budding and boll-forming stages (before topping), with a water concentration of 40 L / mu (approximately 0.067 hectares), with each treatment covering 10 mu (approximately 6.7 hectares). Yield was measured in the experimental areas before harvest. Three plots with uniform growth were randomly selected from each treatment, with each plot measuring 6.67 m². 2 The number of plants and total number of bolls per unit area were investigated. The weight of 100 cotton bolls was measured, the average weight per boll was calculated, and then the yield per mu (unit of land area) was calculated. Data were analyzed using DPS 7.05 software and Duncan's new multiple range test (DMRT method, p = 0.05).

[0151] The results are shown in Table 8. The results indicate that the calcium cyclamate-thiamethoxam-mepiride composition of the present invention, when applied twice during the budding and boll-forming stages, has a better effect on promoting cotton boll number and yield, significantly superior to the effect of a single application. Furthermore, the composition of the present invention, whether applied once or twice, has a better effect on promoting cotton boll number and yield than the conventional agent mepiride. Therefore, the calcium cyclamate-thiamethoxam-mepiride composition has a significantly better yield-increasing effect in cotton fields than conventional treatments, and the two applications during the budding and boll-forming stages have a better regulatory effect on cotton than a single application.

[0152] Table 8. Results of regional trials on the impact of the composition on cotton yield in cotton fields

[0153]

[0154]

[0155] Note: Different letters in the subheadings in Table 8 represent significant differences between groups (P < 0.05), while the same letter represents no significant difference.

[0156] Test Example 2.4: Large-scale cotton field trial

[0157] The experiment was conducted in Jiujianlou Township, Kuitun City, Xinjiang, where cotton growth was generally uniform. Foliar spraying was applied three times: at the initial flowering stage, at the boll-forming stage (7 days before and 7 days after topping), with a water concentration of 40 L / mu (approximately 0.067 hectares), and each treatment covered 10 mu (approximately 6.7 hectares). Before harvest, cotton yield was measured in the experimental area. Three plots with uniform growth were randomly selected from each treatment, with each plot measuring 6.67 m². 2 The number of plants and total number of bolls per unit area were investigated. 100 cotton bolls were weighed to calculate the average weight per boll, and then the yield per mu (unit of land area) was calculated. Data were analyzed using DPS 7.05 software and Duncan's new multiple range test (DMRT method, p = 0.05).

[0158] The results are shown in Table 9. The results indicate that the calcium cyclohexane-thiamethoxam-mepiride composition of the present invention, when applied three times during cotton planting, significantly promoted the number of bolls and yield of cotton plants compared to conventional mepiride treatment. Therefore, multiple applications of the calcium cyclohexane-thiamethoxam-mepiride composition have a significant effect on increasing cotton yield.

[0159] Table 9. Results of regional trials showing the impact of repeated application of the compound on cotton yield in cotton fields.

[0160]

[0161] Note: Different letters in the subscripts in Table 9 represent significant differences between groups (P < 0.05), while the same letter represents no significant difference.

[0162] The greatest advantage of the plant growth regulator composition provided by this invention is that it regulates cotton growth, shapes an ideal plant shape, prunes the topping, and ultimately promotes increased cotton yield, with significant synergistic effects.

[0163] The plant growth regulator composition of this invention can be applied by foliar spraying, aerial application, or mechanical spraying. When using this plant growth regulator composition, in addition to using the active ingredients directly, the composition containing the target active ingredient can be formulated, or the active ingredients can be formulated separately and then mixed. Other fertilizers can also be added for mixing. During application, the active ingredients of this application can be applied separately or simultaneously. It can be applied once during the cotton growth period or multiple times at different stages, making it flexible and convenient to use.

[0164] Table 2 shows that calcium cyclohexane, thiabendazole, and mepiquat chloride, when in a weight ratio of (6–30):(0.075–0.2):(7.5–187.5), all have synergistic effects on cotton growth regulation, topping, and yield increase. This is especially true at a ratio of 1:(0.0025–0.034):(0.25–31.25), preferably 1:(0.0025–0.033):(0.25–31.25), preferably 1:(0.0025–0.034):(0.25–10.5), preferably 1:(0.0025–0.033):(0.25–10.42), preferably 1:(0.0025–0.012):(0.25–10.42), preferably 1:(0.0025–0.012):(0.25–10.42). The ratios are 0.0025~0.011):(0.25~10.42), preferably 1:(0.0025~0.011):(0.25~6.25), preferably 1:(0.0025~0.0067):(0.25~6.25), preferably 1:(0.0025~0.0067):(1.25~6.25), especially showing good synergistic effects in 18:0.15:11.76 (1:0.0083:0.65), 18:0.15:8.85 (1:0.0083:0.49), 18:0.15:44.1 (1:0.0083:2.45), and 18:0.15:176.4 (1:0.0083:9.8).

[0165] The composition of this invention is primarily applied to cotton; there are no strict limitations on the application window, and it can be applied during the cotton growth period; the growth period includes the cotton bud stage, initial flowering stage, and boll-forming stage. It can be applied once or multiple times at different growth stages of cotton.

[0166] The composition of the present invention can be applied by foliar spraying, or by aerial spraying or mechanical spraying.

[0167] The dosage of the composition of the present invention can be as follows: calcium cyclohexane 6-30 g ai / ha; thiamethoxam 0.075-0.2 g ai / ha; mepiquat chloride 7.5-187.5 g ai / ha.

[0168] The plant growth regulator composition of the present invention containing calcium cyclohexane-thiamethoxam-mepiride can be used to regulate cotton growth, shape ideal plant shape, prune cotton topping, promote yield increase, and improve cotton quality.

[0169] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant growth regulator composition for regulating cotton growth, characterized in that, Its active ingredients include calcium cyclohexane, thiazolinone, and mepiquat chloride, wherein the weight ratio of calcium cyclohexane, thiazolinone, and mepiquat chloride is (0.1-1): (0.001-10)：(0.1-300)。 2. The plant growth regulator composition according to claim 1, characterized in that, The weight ratio of the calcium cyclohexane, thiamethoxam, and mepiquat chloride is (0.2-1):(0.0025-0.0066):(0.25-6.25), optionally 1:(0.001-1):(0.25-31.25), and preferably 1:(0.0025-0.033). (0.25~31.25), preferably 1:(0.0025~0.1):(0.25~12.5), preferably 1: (0.0025~0.034):(0.25~10.5), preferably 1:(0.0025~0.5):(0.25~10.42), preferably 1:(0.0025~0.033):(0.25~10.42), preferably 1:(0.0025~0.1): (0.25~10.42), preferably 1: (0.0025~0.05): (0.25~10.42), preferably (0.0025~0.012): (0.25~10.42), preferably 1: (0.0025~0.01): (0.25~6.25), preferably 1: (0.0025~0.0067): (0.25~6.25), preferably 18: 0.15:11.76, 18:0.15:8.85, 18:0.15:44.1 or 18:0.15:176.

4.

3. The plant growth regulator composition according to claim 1 or 2, characterized in that, The active ingredient accounts for 1% to 99% of the total weight of the composition, optionally 5% to 80%, optionally 5% to 75%, optionally 8% to 60%; Optionally, the dosage of calcium cyclohexane is 2–120 g ai / ha, preferably 3–60 g ai / ha, and more preferably 6–30 g ai / ha; Optionally, the dosage of thiamethoxam is 0.025–0.8 g ai / ha, preferably 0.0375–0.4 g ai / ha, and more preferably 0.075–0.2 g ai / ha; Optionally, the dosage of mepiquat chloride is 2.5–750 g ai / ha, preferably 3.75–375 g ai / ha, and more preferably 7.5–187.5 g ai / ha.

4. The plant growth regulator composition according to any one of claims 1 to 3, characterized in that, The formulation of the plant growth regulator composition is one or more of the following: wettable powder, water-dispersible granules, dry suspension, water suspension, dispersible oil suspension, and granules. Optionally, the plant growth regulator composition further includes adjuvants, which are selected from one or more of the following: wetting agents, dispersants, fillers, emulsifiers, thickeners, dispersion media, disintegrants, solvents, preservatives, antifreeze agents, specific gravity regulators, defoamers, oil-phase wall materials, aqueous-phase wall materials, and water.

5. The plant growth regulator composition according to any one of claims 1 to 4, characterized in that, The plant growth regulator composition is in the form of a wettable powder; Optionally, the plant growth regulator composition comprises the following raw material components in weight percentage: The effective ingredient content is 5-75%, optionally 9%-60%; Wetting agent 1-5%, optionally 2-4%; Dispersant 2-15%, optionally 4-10%, optionally 5-8%; The filler content is 20-85%, optionally 30-85%, optionally 31-81%, optionally supplemented to 100%; Preferably, the plant growth regulator composition comprises the following components by weight percentage: 9-60% active ingredient, 2-4% wetting agent, 5-8% dispersant, and the remainder being filler; Preferably, the plant growth regulator composition comprises the following components in weight percentage: 6-36% calcium cyclohexane, 0.05-0.3% thiamethoxam, 2.95-23.52% mepiquat chloride, 2-4% wetting agent, 5-8% dispersant, and filler balance; Optionally, the wetting agent is selected from one or more of the following: dodecyl naphthalene sulfonate, fatty alcohol polyoxyethylene ether, calcium dodecylbenzene sulfonate, sodium dodecyl sulfate, phenol sulfonate, sodium diisobutylnaphthalene sulfonate, polyoxyethylene ether phosphate, etc. Optionally, the dispersant includes one or more of alkyl naphthalene sulfonate formaldehyde condensate, lignin sulfonate, polycarboxylate, alkyl naphthalene sulfonate, etc. Optionally, the filler includes one or more of silica, ammonium sulfate, and kaolin; or, alternatively, the filler includes at least two of silica, ammonium sulfate, and kaolin.

6. The plant growth regulator composition according to any one of claims 1 to 4, characterized in that, The plant growth regulator composition is in the form of water-dispersible granules; Optionally, the plant growth regulator composition comprises the following raw material components in weight percentage: The active ingredient content is 10-70%, optionally 12%-67%; Wetting agent 0-10%, optionally 0-4%; The dispersant is 1-15%, optionally 2-10%, optionally 4-10%, optionally 6-10%; The filler content is 10-80%, optionally 21.65-81.55%, optionally filled to 100%; Optionally, the plant growth regulator composition comprises the following components by weight percentage: 12-67% active ingredient, 0-4% wetting agent, 2-10% dispersant, and the remainder being filler; Preferably, the plant growth regulator composition comprises the following components in weight percentage: calcium cyclohexane 3.6-7.5%, thiamethoxam 0.03-0.05%, mepiquat chloride 8.82-58.8%, wetting agent 0-4%, dispersant 2-10%, and filler balance; Optionally, the wetting agent is one or more of the following: dodecyl naphthalene sulfonate, fatty alcohol polyoxyethylene ether, calcium dodecylbenzene sulfonate, sodium dodecyl sulfate, phenol sulfonate, sodium diisobutylnaphthalene sulfonate, polyoxyethylene ether phosphate, etc. Optionally, the dispersant includes one or more of alkyl naphthalene sulfonate formaldehyde condensate, lignin sulfonate, polycarboxylate, alkyl naphthalene sulfonate, etc. Optionally, the filler includes one or more of corn starch, ammonium sulfate, and kaolin; alternatively, the filler includes at least two of silica, ammonium sulfate, and kaolin.

7. A method for preparing the plant growth regulator composition according to claim 5, characterized in that, Mix calcium cyclohexane, thidiazuron, mepiquat chloride, wetting agent, dispersant and filler evenly, then pulverize by air jet milling until the average particle size D50 is below 5 micrometers, and mix evenly again to obtain the final product.

8. A method for preparing the plant growth regulator composition according to claim 6, characterized in that, Mix calcium cyclohexane, thiamethoxam, mepiquat chloride, wetting agent, dispersant and filler evenly, then pulverize by air jet milling until the average particle size D50 is less than 5 micrometers, add water to knead, extrude and granulate, dry and sieve to obtain the final product; Optionally, add 10% water and knead for 20-50 minutes.

9. The application of a plant growth regulator composition according to any one of claims 1 to 6, or a formulation prepared by the preparation method according to claim 7 or 8, in regulating cotton growth, topping, and increasing yield.

10. A method of using a plant growth regulator composition according to any one of claims 1 to 6, or a formulation prepared by the preparation method according to claim 7 or 8, characterized in that, The plant growth regulator composition is applied during the cotton growing season, optionally once or multiple times at any stage of the cotton growing season, such as the bud stage, the initial flowering stage, or the boll-forming stage. Optionally, the dosage of calcium cyclohexane is 6–30 g ai / ha; Optionally, the dosage of thiamethoxam is 0.075–0.2 g ai / ha; Optionally, the dosage of mepiquat chloride is 7.5–187.5 g ai / ha.

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