Application of BA biological bacteria in animal husbandry

Abstract

The invention relates to the technical field of animal husbandry, and discloses application of BA biological bacteria in animal husbandry, including application in cow breeding, application in pig breeding, application in broiler chickens and laying hens and application in beef cattle breeding. The BA biological bacterium is used as a livestock antibiotic substitute, can effectively prevent and treat livestock and poultry diseases such as cow mastitis and avian influenza, realizes whole-course replacement of antibiotics, avoids residues of the antibiotics in livestock and poultry bodies, enables various indexes of livestock and poultry products to be far higher than the national green food standard, and guarantees the safety of the livestock and poultry products from the source.

Description

Technical Field

[0001] This invention relates to the field of animal husbandry technology, and more specifically, to the application of BA (biota-associated bacteria) in animal husbandry. Background Technology

[0002] Food safety and ecological environment safety are the fundamental guarantees for people to obtain a healthy life. However, the long-term and extensive use of chemical drugs, pesticides and fertilizers in modern agriculture and animal husbandry has led to pesticide residues entering the bodies of livestock and poultry through feed, resulting in a continuous decline in their immunity. This, in turn, forces the medical community to continuously research new antibiotics and drugs for livestock and poultry breeding to prevent infection, forming a vicious cycle, a "sword of Damocles" that threatens the health of all life on Earth. The direct consequence for humanity is a food safety crisis. This short-sighted approach may ultimately lead to the irreversible destruction and damage of humanity and the environment on which they depend.

[0003] my country's livestock industry lags behind that of developed countries, relying almost entirely on vaccines for disease prevention and control in farmed animals, resulting in severe antibiotic overuse. Currently, domestic farms face numerous problems: low costs lead to poor farming environments (especially high-density farming) and low-quality feed ingredients, making animals susceptible to disease and necessitating the use of large quantities of antibiotics. Antibiotics, in turn, affect the animal's digestive system, causing gut microbiota imbalance and weakened immunity, which in turn leads to the excessive use of drugs and hormones. This vicious cycle results in ever-increasing dosages of antibiotics and hormones.

[0004] Long-term consumption of antibiotic-containing foods can gradually increase antibiotic resistance in the human body, essentially planting a "hidden bomb" that could render illness incurable. Besides direct consumption, antibiotics abused in animal husbandry and aquaculture can also transfer to the human body through ecological cycles, causing bacteria to develop antibiotic resistance unknowingly. The food safety problems caused by antibiotic abuse have reached a point concerning the survival of the nation; finding alternative solutions is urgently needed.

[0005] Probiotics, also known as microecological regulators, ecological products, or live bacteria preparations, are live microbial preparations that promote the ecological balance of the intestinal flora and have beneficial effects on the host. Feed probiotics can maintain the microecological balance in the intestines of livestock and poultry. Simultaneously, probiotics produce hydrolytic enzymes and fermentative enzymes that help degrade proteins, fats, and complex carbohydrates in feed. Probiotics can enhance the immune function of livestock and poultry, synthesize bacteriocins, organic acids, lysozyme, hydrogen peroxide, and other substances, and have antagonistic effects against harmful bacteria. Some aerobic probiotics consume oxygen in the intestines, creating an anaerobic environment that promotes the growth of anaerobic microorganisms, thus restoring the imbalanced flora to a normal state. Probiotics also have functions such as synthesizing B vitamins, inhibiting the production of harmful substances, producing antibiotic-like substances, and forming an intestinal barrier.

[0006] BA probiotics are a type of beneficial bacteria that can survive under both anaerobic and aerobic conditions, developed over several years of research and large-scale experiments by Professor Iwao Uebayashi and researchers including Tsukasa Namikawa and Bikai Pan at the Toyo Biological Research Institute in Japan. This research represents a breakthrough in overcoming the challenge of symbiotic relationships among complex microbial communities in the field of bioscience, achieving successful compounding of specific beneficial microorganisms. As an alternative to antibiotics in poultry and livestock, BA probiotics significantly outperform currently available probiotic feed products. This strain can proliferate under both anaerobic and aerobic conditions, making it suitable for establishing new pollution control and safe breeding technology systems. Based on the excellent characteristics of BA probiotics, their application in animal husbandry holds promise for solving many problems currently facing the industry, such as antibiotic overuse. Summary of the Invention

[0007] In view of this, the present invention proposes the application of BA bacteria in animal husbandry, aiming to solve at least one of the current background technologies.

[0008] This invention proposes the application of BA (biota-associated bacteria) in animal husbandry.

[0009] Preferably, the application includes dairy farming, specifically: for mastitis in dairy cows, a BA bacteria active liquid at EC1.1 concentration is diluted 600 times and given to dairy cows for direct drinking, 2-3 times a day, for 5-7 consecutive days as one course of treatment; during the lactation period of dairy cows, a BA bacteria active liquid at EC1.1 concentration is diluted 600 times and added to the dairy cows' feed and drinking water, with the daily dosage of the BA bacteria active liquid being 100-150 ml / 100 kg.

[0010] Preferably, it also includes application in pig farming, specifically: from the beginning of the fattening period of fattening pigs until slaughter, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of the fattening pigs, and the daily usage of the BA bacteria active liquid is 80-120ml / 100kg.

[0011] Preferably, it also includes application in breeding pigs, specifically: continuously diluting the EC1.1 concentration of BA bacteria active liquid 600 times throughout the year and adding it to the feed and drinking water of breeding pigs, with the daily usage of the diluted BA bacteria active liquid being 100-150 ml / kg.

[0012] Preferably, it also includes applications in broiler farming, specifically including: For broilers, from the chick stage to slaughter, dilute EC1.1 concentration of BA active liquid 600 times and add it to the broiler feed and drinking water. During the chick stage, use 5-10ml of diluted BA active liquid per bird per day. During the growing stage, use 10-15ml of diluted BA active liquid per bird per day. During the adult stage, use 15-20ml of diluted BA active liquid per bird per day.

[0013] Preferably, applications in egg-laying hen farming are also included, specifically: For laying hens, from the start of laying until culling, dilute EC1.1 concentration of BA bacteria active solution 600 times and add it to the feed and drinking water of the laying hens. The daily usage of diluted BA bacteria active solution is 10-15ml / hen.

[0014] Preferably, it also includes application in beef cattle farming, specifically: from the beginning of the fattening period of beef cattle until slaughter, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of beef cattle, and the daily usage of the BA bacteria active liquid is 120-140ml / 100kg.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) Replacing antibiotics and ensuring food safety: In this invention, BA bacteria can effectively prevent and treat livestock diseases such as mastitis in dairy cows and avian influenza, thus achieving full replacement of "antibiotics" and avoiding antibiotic residues in livestock. This ensures that all indicators of livestock products are far higher than the national green food standards, thus guaranteeing the safety of livestock products from the source.

[0016] (2) Improving livestock and poultry immunity and production performance: BA bacteria can enhance the immune function of livestock and poultry, maintain the microecological balance in the intestines of livestock and poultry, and promote the digestion and absorption of nutrients. In pig farming, fattening pigs have high nutritional content (high calcium, high protein, low cholesterol, low fat), saving about 10% of feed and significantly improving feed conversion ratio; breeding pigs can improve milk quality, increase litter size and piglet survival rate, and enhance the immunity of piglets, resulting in rapid weight gain. In beef and dairy cattle farming, beef cattle gain weight, improve physical condition, and enhance immunity, reducing feed conversion ratio, increasing weight gain rate, reducing feeding costs, and increasing cattle farming efficiency; dairy cows increase milk production and milk quality, increase milk production and milk protein content during lactation, increase milk fat percentage, and significantly enhance the aroma of milk, while greatly reducing the chance of dairy cows developing mastitis. In broiler and laying hen farming, broiler feed is significantly reduced, returning to normal taste; the overall feeding cost per pound of eggs for laying hens is reduced.

[0017] (3) Reduce breeding costs: The use of BA bacteria can improve feed utilization and save feed, while reducing the use of antibiotics, hormones and other drugs, thus reducing drug costs in the breeding process. In addition, BA bacteria have low production costs and can be produced on a large or small scale, which greatly reduces equipment investment in the production process, simplifies the relevant production process, and further reduces the overall breeding cost.

[0018] (4) Promote ecological cycle: The manure produced by breeding is odorless and has little residual nutrients under the action of BA bacteria. There is almost no space for other bacteria to survive. It can be used as biological organic fertilizer to improve the soil, repair and improve the land, achieve the purpose of green planting and quality improvement and efficiency enhancement, and promote the ecological cycle of breeding and planting. Detailed Implementation

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0020] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included within this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0021] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0022] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0023] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0024] This invention proposes the application of BA (biota-associated bacteria) in animal husbandry.

[0025] BA microbiota is a complex flora composed of 12 species from 8 genera, including Lactobacillus, Bacillus, and Saccharomyces. These strains maintain a stable microecological balance through the interaction of their metabolic products. Electron microscopy reveals that the Lactobacillus species are short rod-shaped (0.5-1.0 μm × 2.0-4.0 μm), Gram-positive, and non-spore-forming; the Bacillus species are long rod-shaped (0.6-1.0 μm × 2.0-5.0 μm), with flagella all over their bodies, and can form elliptical spores; the Saccharomyces species are round or elliptical (3-5 μm × 5-10 μm), exhibiting significant budding reproduction. This diverse bacterial structure allows them to colonize and reproduce in the anaerobic environment of the livestock gut, while also functioning under aerobic conditions in the breeding environment, laying a biological foundation for the complete replacement of antibiotics throughout the entire breeding process.

[0026] In this invention, the application in dairy farming is specifically as follows: For mastitis in dairy cows, a BA bacteria active liquid with an EC1.1 concentration is diluted 600 times and given to dairy cows for direct drinking, 2-3 times a day, for 5-7 consecutive days as one course of treatment; During the lactation period of dairy cows, a BA bacteria active liquid with an EC1.1 concentration is diluted 600 times and added to the dairy cows' feed and drinking water, with the daily dosage of the BA bacteria active liquid being 100-150 ml / 100 kg.

[0027] Specifically, in dairy farming, the application method can be to establish a graded treatment system based on the cow's disease condition, and adjust the use of BA bacteria accordingly, such as: Mild infection (somatic cell count 100,000-200,000 / mL): Use EC1.1 concentration BA bacteria active solution diluted 600 times, drink 500mL 3 times a day, for 5 consecutive days as one course of treatment; For moderate infection (somatic cell count 200,000-500,000 / mL): In addition to oral administration, administer 300 times diluted BA bacteria active solution to the breast (50mL per breast area) twice daily for up to 7 days. Severe infection (somatic cell count > 500,000 / mL): Use a triple therapy of "oral + irrigation + medicated bath". The medicated bath solution is a 200-fold dilution of BA bacteria and is used after milking every day.

[0028] During the lactation period of dairy cows, a precise feeding system can be established based on the cow's weight and milk production, such as: Body weight 500-600kg, daily milk production 20-25kg: Add 100mL of BA bacteria active solution (600 times diluted) twice daily, morning and evening, mixed into the TMR feed. For animals weighing 600-700kg and producing 25-30kg of milk per day: add 120mL daily, administering the medication via a dual-channel approach of "feed + water" (each accounting for 50%). For animals weighing over 700kg and producing more than 30kg of milk per day: add 150mL daily, fed through a special dosing device within 30 minutes after milking.

[0029] Those skilled in the art can also adjust the dosage and frequency of BA bacteria according to the actual application.

[0030] The specific application of the BA microorganisms described in this invention in beef and dairy cattle farming results in increased weight, improved physical condition, and enhanced immunity in beef cattle, reduced feed conversion ratio, increased weight gain rate, lower feeding costs, and increased cattle farming efficiency. In dairy cows, it improves milk production and dairy product quality, increases milk yield and milk protein content during lactation, improves milk fat percentage, and significantly enhances the milk's aroma and flavor. Simultaneously, it greatly reduces the incidence of mastitis in dairy cows. This invention also includes its application in pig farming, specifically: from the start of the fattening period until slaughter, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of the fattening pigs. The daily usage of the BA bacteria active liquid is 80-120 ml / 100 kg.

[0031] Specifically, a refined plan is formulated according to the growth cycle of fattening pigs: Nursery period (20-35kg): 80mL / 100kg body weight of BA active liquid per day, diluted 600 times and mixed into creep feed, fed 4 times a day; Growth period (35-70kg): 100mL / 100kg body weight per day, using a "dry and wet feed + drinking water" compound addition mode; Fattening period (70kg to slaughter): 120mL / 100kg body weight per day, evenly mixed into complete feed to ensure that each bite contains bacterial liquid.

[0032] BA (Bacterial Acid) bacteria can enhance the immune function of livestock and poultry, maintain the microecological balance in their intestines, and promote the digestion and absorption of nutrients. In pig farming, fattening pigs produce pork with high nutritional content (high calcium, high protein, low cholesterol, low fat), saving about 10% of feed and significantly improving feed conversion ratio.

[0033] This invention also includes its application in breeding pigs, specifically: continuously diluting EC1.1 concentration BA bacterial active liquid 600 times and adding it to the feed and drinking water of breeding pigs throughout the year, wherein the daily usage of the BA bacterial active liquid is 100-150 ml / kg of diluted BA bacterial active liquid.

[0034] In this invention, the application of BA bacteria in breeding pigs is divided into boar feeding management and sow feeding management, specifically as follows: For boars: Adult boars (>12 months old): Add 150mL of BA bacteria active liquid per 100kg body weight daily, divided into two feedings in the morning and evening; Replacement boars (6-12 months old): 120 mL / 100 kg body weight per day, increasing to 150 mL 30 days before mating. For sows: During the non-pregnant period: 100mL / 100kg body weight daily to promote estrus and follicle development; During pregnancy: 120mL / 100kg in the first trimester (days 1-84) and 150mL / 100kg in the second trimester (days 85 to delivery) to reduce embryonic mortality. During lactation: 150-200mL / 100kg body weight per day to increase milk production and quality; For piglets: Within 24 hours of birth: Administer 1 mL of BA bacterial active solution (300 times diluted) orally to enhance immunity; Supplemental feeding at 7 days old: Add 600 times diluted BA bacteria solution to the starter feed, 5 mL per animal per day; Weaning transition period (21-28 days old): 10 mL per head per day, divided into 3 feedings, to reduce weaning stress.

[0035] The application of BA (Bacterial Acid) bacteria in pig breeding can improve milk quality, increase litter size and piglet survival rate, enhance piglets' immunity, and promote rapid weight gain.

[0036] This invention also includes applications in broiler farming, specifically including: For broilers, from the chick stage to slaughter, dilute EC1.1 concentration of BA active liquid 600 times and add it to the broiler feed and drinking water. During the chick stage, use 5-10ml of diluted BA active liquid per bird per day. During the growing stage, use 10-15ml of diluted BA active liquid per bird per day. During the adult stage, use 15-20ml of diluted BA active liquid per bird per day.

[0037] In this invention, the preferred application in broiler farming is to formulate a dynamic supplementation method based on the differences in nutritional requirements of broilers at different growth stages: Chick stage (1-14 days old): 5-10 mL of diluted solution (600 times) per chick per day, supplied through a chick-specific drinker to ensure that each chick consumes it evenly; Medium-sized chickens (15-28 days old): 10-15 mL per bird per day, using a dual channel of "drinking water + mixed feed" (each accounting for 50%), to adapt to the increased feed intake; Large chicken stage (29 days old to slaughter): 15-20mL per chicken per day, mainly mixed with feed (accounting for 70%), to reduce the risk of water system blockage.

[0038] This invention also includes applications in egg-laying hen farming, specifically including: For laying hens, from the start of laying until culling, dilute EC1.1 concentration of BA bacteria active solution 600 times and add it to the feed and drinking water of the laying hens. The daily usage of diluted BA bacteria active solution is 10-15ml / hen.

[0039] In this invention, the preferred application in egg-laying hen farming is to formulate a precise addition method based on the characteristics of the egg production curve of egg-laying hens: During the egg-laying period (18-25 weeks of age): Start by adding 10 mL of diluted solution per bird per day at a 5% egg production rate to promote a rapid increase in egg production. Peak laying period (26-45 weeks of age): Increase to 12-15 mL / bird / day, maintaining peak egg production (>90%) for a period of time. Late laying period (46 weeks of age to culling): Maintain 10-12 mL / bird / day to slow down the rate of decline in egg production.

[0040] This invention also includes its application in beef cattle farming, specifically: from the start of the fattening period of beef cattle until slaughter, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of the beef cattle. The daily usage of the BA bacteria active liquid is 120-140 ml / 100 kg.

[0041] Further, preferably: mix EC1.1 concentration BA bacteria active liquid with clean drinking water at a volume ratio of 1:600, stir evenly, and let stand for 10 minutes to ensure that the bacterial liquid is fully activated; the diluted bacterial liquid should be used within 24 hours, avoiding direct sunlight and high temperature environment (storage temperature controlled at 5-25℃); feed addition: add 120-140ml / 100kg body weight of BA bacteria active liquid (calculated according to the weight of beef cattle on the day) into the total mixed ration (TMR) every day, stir for 3-5 minutes until evenly distributed; in the early fattening stage (300-450kg), provide 120ml / 100kg body weight, and in the late fattening stage (450kg to slaughter), provide 140ml / 100kg body weight.

[0042] Water supplementation: Add the same amount of BA bacteria diluted solution as in the feed to the beef cattle's drinking system daily, and provide it twice a day, morning and evening (50% each at 8:00 am and 4:00 pm), to ensure that each beef cattle drinks no less than 50L of water per day to ensure sufficient intake of the bacteria solution.

[0043] In this invention, BA bacteria, as a substitute for antibiotics in poultry and livestock, can effectively prevent and treat diseases such as mastitis in dairy cows and avian influenza. It achieves a complete replacement of antibiotics throughout the entire process, avoids antibiotic residues in livestock and poultry, and ensures that all indicators of livestock and poultry products are far higher than the national green food standards, thus guaranteeing the safety of livestock and poultry products from the source.

[0044] Specifically, BA bacteria are applied to animal husbandry in the following ways: (1) Reshaping the intestinal health of livestock and poultry through a dual pathway of "occupation competition" and "metabolic inhibition": The strain can specifically bind to receptors on the surface of intestinal epithelial cells (such as mannose receptors) through adhesion factors such as pili, forming a biofilm barrier. The colonization rate of the intestinal mucosa can reach more than 85% over a long period of time, significantly inhibiting the attachment of pathogens; the organic acids produced can reduce the pH value of the intestine from 6.5 to about 5.0, destroying the survival environment of pathogens; bacteriocins can target the cell membrane of pathogens, causing their permeability to increase and leading to lysis and death. It has a significant restorative effect on intestinal flora imbalance caused by antibiotic abuse. After feeding BA bacteria for 30 days, the number of lactic acid bacteria in the intestines of broilers can be significantly increased, the number of Escherichia coli can be significantly decreased, and the ratio of Firmicutes to Bacteroidetes can be restored to a healthy level. (2) BA bacteria comprehensively enhance the disease resistance of livestock and poultry through the synergistic activation of non-specific and specific immunity: Innate immunity activation: stimulates macrophages in the lamina propria of the intestinal mucosa to release cytokines (IL-2, IL-6, TNF-α, etc.); Adaptive immunity enhancement: promotes the proliferation of spleen T lymphocytes and the production of antibodies by B lymphocytes, significantly increasing the neutralizing antibody titer against pathogens such as avian influenza virus; Mucosal immunity enhancement: increases the number of goblet cells and the concentration of secretory immunoglobulin A (sIgA) in the intestinal mucosa, constructing the first line of defense against pathogen invasion. (3) BA bacteria significantly improve the nutritional value of feed by secreting a variety of digestive enzymes: protein digestion: the protease produced can break down the large molecular protein in soybean meal into small molecular peptides and free amino acids, thereby improving the protein utilization rate; carbohydrate degradation: amylase and cellulase work together to break down the starch in corn and the cellulose in wheat bran into glucose and cellobiose, thereby increasing the energy metabolism rate by 15%-20%; mineral absorption promotion: the organic acids produced by metabolism can significantly increase the solubility of minerals such as calcium and phosphorus in feed.

[0045] In animal husbandry, the use of BA (bioactive bacteria) can improve feed utilization, save feed, and reduce the use of antibiotics, hormones, and other drugs, thus lowering drug costs in the breeding process. Furthermore, BA has low production costs and can be scaled up or down, significantly reducing equipment investment and simplifying the production process, further lowering overall breeding costs.

[0046] In livestock farming, the manure produced by livestock breeding, under the action of BA bacteria, is odorless and has few residual nutrients. It has almost no space for other bacteria to survive and can be used as bio-organic fertilizer to improve soil, restore and improve land, achieve the purpose of green planting and improve quality and efficiency, and promote the ecological cycle of breeding and planting industries.

[0047] Example 1: Application of BA bacteria in dairy farming (1) Experimental subjects and grouping Mastitis Prevention and Treatment Group: 150 Holstein cows with mastitis were selected and divided into three groups based on somatic cell count (SCC): Mild infection group (SCC 100,000-200,000 / mL): 50 piglets, average parity 2.3, daily milk production 22 kg Moderate infection group (SCC 200,000-500,000 / mL): 50 piglets, average parity 2.8, daily milk production 19 kg Severe infection group (SCC>500,000 / mL): 50 piglets, average parity 3.1, daily milk production 16kg Each group was then randomly divided into an experimental group (25 animals) and a control group (25 animals). Lactation performance group: 100 healthy lactating dairy cows (SCC < 100,000 / mL), with an average parity of 2.5 and a daily milk yield of 25 kg were selected and randomly divided into an experimental group (50 cows) and a control group (50 cows). (2) Test methods Mastitis Prevention and Treatment Plan: Experimental group: All patients were uniformly treated with EC1.1 concentration BA bacterial active solution diluted 600 times, using a combined "oral + local perfusion" approach. Mild infection: Oral administration 500mL three times daily for 5 consecutive days. Moderate infection: Oral administration 3 times daily (500mL each time) + mammary infusion (50mL per breast area, twice daily) for 7 consecutive days. Severe infection: Oral administration 3 times daily (500mL each time) + mammary irrigation (50mL per mammary area, twice daily) + nipple bath (200x diluted solution, used after milking), for 10 consecutive days. Control group: Treated with conventional antibiotics (ceftiofur sodium + ofloxacin), administered according to the instructions. (3) Lactation performance enhancement program: Experimental group: Throughout the lactation period, 600 times diluted EC1.1 concentration BA bacteria active liquid was added to feed and drinking water, 120 mL per head per day (80 mL in feed and 40 mL in drinking water). Control group: fed according to standard feeding methods, with the same amount of physiological saline added to their feed. (4) Test indicators and detection frequency Mastitis markers: Daily record of breast redness and swelling, somatic cell count in breast milk (tested twice a week), and pathogen count (tested on days 0, 3, 7, and 10). Lactation indicators: Daily record of milk production, weekly testing of milk components (milk protein, milk fat, lactose), and monthly testing of antibiotic residues in breast milk. (5) The test results are shown in Table 1: Table 1. Detection Results of BA Bacteria in Dairy Farming

[0048] (6) The results of the lactation performance improvement effect are as follows: Milk production: The average daily milk production of the experimental group was 27.3 kg, which was 9.2% higher than that of the control group (25.1 kg). The most significant increase was observed in the third month of lactation (11.5%). Milk composition: Milk protein increased from 3.12% to 3.58%, milk fat increased from 3.75% to 4.12%, and lactose content remained stable (4.8-4.9%). Breast health: The incidence of mastitis in the experimental group was only 3.2%, a decrease of 82.7% compared to the control group (18.5%). Based on the above, it can be seen that the application of BA bacteria in dairy farming can effectively prevent and treat mastitis in dairy cows, significantly reduce the incidence of mastitis, and improve lactation performance, making it suitable for widespread application in dairy farming.

[0049] Example 2: Application of BA bacteria in pig farming (1) Experimental subjects and grouping Fattening pig experiment: 300 Duroc-Landrace-Landrace-Landrace-Cross-Breeder crossbred pigs (initial weight 35±2kg) were randomly divided into 3 groups: Experimental group 1: 100 heads, using 80mL / 100kg body weight of BA bacteria active solution. Experimental group 2: 100 animals, treated with 100 mL of BA bacteria active solution per 100 kg body weight Control group: 100 animals, fed using standard feeding methods (with the same amount of physiological saline added). Breeding pig trial: Sixty Landrace breeding pigs (30 boars and 30 sows) were selected and randomly divided into an experimental group (30 pigs) and a control group (30 pigs). Experimental group: 120 mL / 100 kg body weight of BA bacteria active solution was added throughout the year. Control group: Routine feeding Piglet experiment: 200 weaned piglets at 21 days of age (weight 6.5±0.5kg) were randomly divided into an experimental group (100 piglets) and a control group (100 piglets). Experimental group: 10 mL of diluted BA bacteria solution was added to each animal daily. Control group: fed with standard nursery feed (2) Test methods For fattening pigs: from 35kg to 110kg for slaughter, dilute BA bacteria active solution 600 times and mix it into complete feed, feed 3 times a day. Breeding pigs: Boars are fed twice a day, while sows are fed according to their physiological stages (100mL during the non-pregnant period, 120mL during the gestation period, and 150mL during the lactation period). Piglets: Administer BA bacteria diluted solution orally twice a day, morning and evening. After the transition period (21-28 days old), mix it into the nursery feed. (3) Test indicators Fattening pigs: daily weight gain, feed conversion ratio, slaughter performance (lean meat percentage, backfat thickness), meat quality (cholesterol, fatty acid composition). Breeding pigs: boar sperm motility, sow litter size, piglet survival rate, weaning weight Piglets: Diarrhea rate, daily weight gain, survival rate, and immune indicators (IgG content). The experimental results are shown in Table 2. Table 2. Results of the application experiment of BA bacteria in pig farming

[0050] Reproductive performance of breeding pigs: Boars: The sperm motility in the experimental group was 85.6%, an increase of 18.4% compared to the control group (72.3%), and the sperm deformity rate was reduced by 52%. Sows: The experimental group had an average litter size of 11.8 live piglets, which was 15.7% higher than the control group (10.2 piglets). The weaning survival rate of piglets was 96.5% (compared to 88.3% in the control group). Piglet care effectiveness: The diarrhea rate in the experimental group was only 4.2%, a decrease of 85.3% compared to the control group (28.5%). The average daily weight gain was 285g, a 35.7% increase compared to the control group (210g). Serum IgG levels were 18.5 mg / mL, a 50.4% increase compared to the control group (12.3 mg / mL). Based on the above, it can be seen that BA bacteria can improve the growth performance and pork quality of fattening pigs, enhance the reproductive capacity of breeding pigs and the survival rate of piglets, and are an ideal choice to replace antibiotics and optimize breeding results in pig farming.

[0051] Example 3: Application of BA (Bacterial Acid) bacteria in broiler and layer chicken farming (1) Experimental subjects and grouping Broiler chicken experiment: 600 AA broiler chicks (1 day old) were randomly divided into 3 groups: Experimental group 1: 200 birds, supplemented with 5 mL / bird / day for chicks, 10 mL / bird / day for growing chickens, and 15 mL / bird / day for adult chickens. Experimental group 2: 200 birds, supplemented with 10 mL / bird / day for chicks, 15 mL / bird / day for growing chickens, and 20 mL / bird / day for adult chickens. Control group: 200 birds, fed according to standard feeding methods Laying hen experiment: 400 Lohmann Brown laying hens (120 days old, about to start laying) were randomly divided into 2 groups: Experimental group: 200 birds, 12 mL / bird / day of BA bacteria dilution was added from the start of production until culling. Control group: 200 birds, fed according to standard feeding methods (2) Test methods Broilers: Dilute BA probiotic active solution 600 times. For chicks (1-14 days old), provide via drinking water. For growing chickens (15-28 days old) and adult chickens (29 days old to slaughter), use a 50 / 50 combination of drinking water and feed. Laying hens: Mix BA bacteria diluted solution into feed and feed twice daily (7:00 AM and 5:00 PM). Record egg production rate, egg weight, and egg quality indicators. (3) Test indicators Broiler chickens: survival rate, daily weight gain, feed conversion ratio, dressing percentage, and muscle flavor compounds (inosinic acid, fatty acids). Laying hens: Egg production rate, average egg weight, eggshell strength, yolk color, Haugh unit, feeding cost The results of broiler chicken farming are shown in Table 3: Table 3 Results of Broiler Chicken Farming

[0052] Laying hen production performance: Egg production rate: The peak egg production rate in the experimental group (26-45 weeks of age) was 95.3%, which was 5.8% higher than that in the control group (90.1%), and the peak production period lasted for 6 weeks longer. Egg quality: Eggshell strength 3.65 kg / cm² (control group 3.12 kg / cm²), yolk color Roche grade 5.2 (control group 3.1), Haugh unit 86 (control group 73). Based on the above, it can be seen that BA bacteria can significantly reduce broiler feed consumption, improve survival rate and meat quality, while reducing the cost of laying hen farming and improving egg quality. Its application effect in poultry farming is significant.

[0053] Example 4: Application of BA (Bacterial Acid) bacteria in beef cattle farming (1) Experimental subjects: 100 beef cattle were selected and randomly divided into two groups of 50 each, namely the experimental group and the control group.

[0054] (2) Experimental method: From the beginning of the fattening period to the slaughter, the experimental group of beef cattle were fed and drunk water with BA bacteria active liquid diluted 600 times with EC1.1 concentration. Each beef cattle used 130ml of diluted BA bacteria active liquid per day. The control group of beef cattle were fed using conventional feeding methods without the addition of BA bacteria.

[0055] (3) Experimental results: The average daily weight gain of the experimental group of beef cattle was 12% higher than that of the control group, the feed conversion ratio was 10% lower, the physical condition was significantly improved, the immunity was enhanced, and the incidence rate was 15% lower than that of the control group.

[0056] Based on the above, it can be seen that BA bacteria can promote the growth of beef cattle, improve feed conversion rate, enhance immunity, reduce morbidity, and effectively improve the efficiency of beef cattle farming.

[0057] Example 5: Application of BA biological bacteria in deodorization of livestock pens (1) Experimental methods: Two pens of the same area and breeding scale were selected as the experimental group and the control group, respectively. The experimental group was treated with 500 times diluted BA bacteria solution for fermentation bed treatment. The 500 times diluted BA bacteria solution was sprayed on the fermentation bed twice a week to maintain the humidity of the fermentation bed at about 55%. For the sewage from the breeding farm, BA bacteria were added at a ratio of 1 kg of BA bacteria to treat 2 tons of sewage, and aeration was carried out for 10 hours a day. For domestic waste (including manure treatment), BA bacteria were added at a ratio of one-third of the waste treatment volume for mixed fermentation treatment. The control group pens were cleaned using conventional methods and BA bacteria were not used.

[0058] (2) Experimental results: The odor in the experimental group's enclosures was significantly reduced, the ammonia concentration was 60% lower than that in the control group, and the number of mosquitoes and flies was 70% lower than that in the control group; after treatment, the wastewater showed significant improvement in various indicators and met the discharge standards; after fermentation, the domestic waste (including feces) had no obvious odor and could be used as high-quality biological organic fertilizer. The control group's enclosures had a significant odor, high ammonia concentration, and a large number of mosquitoes and flies.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the specific implementation of the present invention. Any modifications or equivalent substitutions that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. Application of BA biological bacteria in animal husbandry.

2. The application of BA biological bacteria in animal husbandry according to claim 1, characterized in that, This includes applications in dairy farming, specifically: for mastitis in dairy cows, use EC1.1 concentration BA bacteria active liquid diluted 600 times and give it to dairy cows to drink directly, 2-3 times a day, for 5-7 consecutive days as one course of treatment; during the lactation period of dairy cows, add EC1.1 concentration BA bacteria active liquid diluted 600 times to the feed and drinking water of dairy cows, the daily dosage of the BA bacteria active liquid is 100-150ml / 100kg.

3. The application of BA biological bacteria in animal husbandry according to claim 1, characterized in that, It also includes applications in pig farming, specifically: from the start of the fattening period until slaughter, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of the fattening pigs. The daily usage of the BA bacteria active liquid is 80-120 ml / 100 kg.

4. The application of BA biological bacteria in animal husbandry according to claim 3, characterized in that, It also includes its application in breeding pigs, specifically: throughout the year, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of breeding pigs. The daily usage of the diluted BA bacteria active liquid is 100-150 ml / kg.

5. The application of BA biological bacteria in animal husbandry according to claim 1, characterized in that, It also includes applications in broiler farming, specifically including: For broilers, from the chick stage to slaughter, dilute EC1.1 concentration of BA active liquid 600 times and add it to the broiler feed and drinking water. During the chick stage, use 5-10ml of diluted BA active liquid per bird per day. During the growing stage, use 10-15ml of diluted BA active liquid per bird per day. During the adult stage, use 15-20ml of diluted BA active liquid per bird per day.

6. The application of BA biological bacteria in animal husbandry according to claim 1, characterized in that, It also includes applications in egg-laying hen farming, specifically including: For laying hens, from the start of laying until culling, dilute EC1.1 concentration of BA bacteria active solution 600 times and add it to the feed and drinking water of the laying hens. The daily usage of diluted BA bacteria active solution is 10-15ml / hen.

7. The application of BA biological bacteria in animal husbandry according to claim 1, characterized in that, It also includes applications in beef cattle farming, specifically: from the start of the fattening period until slaughter, the EC1.1 concentration of BA bacteria active liquid is diluted 600 times and added to the feed and drinking water of the beef cattle. The daily usage of the BA bacteria active liquid is 120-140 ml / 100 kg.

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