Capsule for relaxing muscles and stimulating blood circulation and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a tendon-relaxing and blood-activating capsule and a preparation method thereof. The capsule for relaxing the muscles and stimulating the blood circulation is prepared from the following raw material components in parts by weight: 20 to 22 parts of flos carthami, 77 to 80 parts of prepared rhizoma cyperi, 103 to 106 parts of prepared rhizoma cibotii, 51 to 54 parts of cortex periplocae, 77 to 79 parts of caulis trachelospermi, 77 to 79 parts of herba lycopodii, 77 to 79 parts of herba lycopi, 103 to 106 parts of mistletoe, 77 to 79 parts of caulis spatholobi and 12 to 14 parts of calcined native copper. The preparation method is improved, and the obtained capsule for relaxing muscles and stimulating blood circulation is high in content of effective components, short in disintegration time, high in bioavailability and capable of effectively reducing the dosage and greatly improving the taking compliance of a patient.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine pharmaceutical technology, specifically relating to a muscle-relaxing and blood-activating capsule and its preparation method. Background Technology

[0002] Shujin Huoxue Capsules are a traditional Chinese medicine with the effects of relaxing muscles and tendons, promoting blood circulation, and removing blood stasis. They are suitable for symptoms such as muscle and bone pain, limb spasms, lower back pain, and sprains. This medicine is included in the National Medical Products Administration's drug catalog and corresponding standards have been provided. The preparation method mainly involves directly pulverizing or decocting ten herbs, including safflower, processed cyperus rotundus, processed cibotium barometz, and crushed licorice root bark, then mixing them with starch, maltose, and ethanol to form granules, which are then dried. Because the active ingredient content of Shujin Huoxue Capsules prepared by this method is relatively low, and the disintegration time is long with low bioavailability, a larger dosage is required, resulting in relatively poor medication adherence.

[0003] Due to some defects in the muscle-relaxing and blood-activating capsules, manufacturers have been continuously improving their preparation processes to obtain products with better performance. For example, the patent application number 200910201799.X describes a preparation method that involves extracting natural copper together with Trachelospermum jasminoides, Lycopodium clavatum, Lycopus lucidus, Mistletoe, and Spatholobus suberectus using water, concentrating the extract into a thick paste, then adding other powdered ingredients without fillers, drying, and encapsulating. While this method conforms to the standards of the State Drug Administration, the main difference is the absence of fillers. Although the amount used is reduced, the bioavailability remains unchanged. Furthermore, extracting natural copper together with other medicinal materials using water can easily reduce the content of the effective components of natural copper. The patent application with application number 201110129493.5 significantly improved the preparation method. It involved pulverizing safflower, processed Cyperus rotundus, processed Cibotium barometz, Periploca sepium, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaf, Mistletoe, and Spatholobus suberectus into a fine powder, then extracting them with 60% ethanol under acidic and alkaline conditions. While acidic conditions are beneficial for the extraction of hydroxysaffron yellow A and trachelospermum jasminoides, they are easily destroyed under alkaline conditions, similarly affecting the extraction efficiency. Furthermore, directly spray-drying the thick paste at high temperatures can also easily destroy the active ingredients due to excessively high temperatures. Therefore, further research is needed to obtain a high-quality muscle-relaxing and blood-activating capsule. Summary of the Invention

[0004] To address the shortcomings of existing technologies, the present invention aims to provide a muscle-relaxing and blood-activating capsule and its preparation method. This muscle-relaxing and blood-activating capsule has a high content of effective ingredients, is easily absorbed, has high bioavailability, can effectively reduce the dosage, and greatly improve medication compliance.

[0005] The first objective of this invention is to provide a method for preparing a muscle-relaxing and blood-activating capsule, wherein the muscle-relaxing and blood-activating capsule comprises the following raw material components in parts by weight: 20-22 parts of safflower, 77-79 parts of prepared Cyperus rotundus, 103-106 parts of prepared Cibotium barometz, 51-54 parts of Periploca sepium, 77-79 parts of Trachelospermum jasminoides, 77-79 parts of Lycopodium clavatum, 77-79 parts of Lycopodium clavatum leaf, 103-106 parts of mistletoe, 77-79 parts of Spatholobus suberectus, and 12-14 parts of calcined natural copper; The preparation method includes the following steps: (1) Prepare the above-mentioned raw materials and set aside; (2) After pulverizing the calcined natural copper, pass it through a 200-mesh sieve. Take the amount of pulverized calcined natural copper according to the formula, add a 1% citric acid solution, mix, and extract under ultrasonic conditions for 1-2 hours. Filter, extract the filter residue once, combine the filtrates, and obtain filtrate a for later use. (3) The safflower, prepared Cyperus rotundus, prepared Cibotium barometz, Cibotium barometz bark, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaves, Mistletoe, and Spatholobus suberectus are pulverized and passed through a 100-mesh sieve. They are then mixed according to the formula to obtain a mixture. Pure water and compound enzyme preparation are added and mixed for 1-2 hours. The mixture is then heated to 80°C and kept warm for 10 minutes. After centrifugation, filtrate b and dregs are obtained. (4) Add organic solvent A to the residue from step (3), extract at 50°C for 0.5-1h, filter to obtain filtrate c, add organic solvent B to the residue, extract at 80°C for 1-2h, filter under pressure to obtain filtrate d; (5) After mixing the filtrates a, b, c and d, the mixture is concentrated under reduced pressure, then dried in three stages, sieved and filled into capsules to obtain the muscle-relaxing and blood-activating capsules.

[0006] This invention improves the preparation method by first pulverizing calcined natural copper and then using citric acid as a dissolving agent in conjunction with ultrasonic extraction, which effectively increases the dissolution rate of iron ions and improves bioavailability. Pretreatment with a compound enzyme preparation breaks down plant cell walls, releasing glycosides. Then, extraction is performed using a mixed solvent at different temperatures: ethyl acetate extracts fat-soluble components such as cyperone and mistletoe flavonoids, while ethanol extracts water-soluble components such as safflower yellow pigment. Using two treatments with different ratios of mixed solvents and different temperatures ensures thorough extraction of the active ingredients from the raw material, increasing their content. A three-stage drying process, all conducted at low temperatures, effectively controls the rate of moisture removal and prevents the decomposition of heat-sensitive components, maximizing the retention of active ingredients. The muscle-relaxing and blood-activating capsules prepared by this invention have high active ingredient content and good bioavailability, allowing for further reduction in dosage and improved patient compliance.

[0007] Furthermore, in step (2), the solid-liquid ratio of calcined natural copper and citric acid solution is 1:10-15, and the ultrasonic conditions are 300W and 70-80℃.

[0008] Further, in step (3), the compound enzyme preparation is a mixture of cellulase and pectinase in a mass ratio of 1-2:1; the amount of the compound enzyme preparation added is 0.5-1% of the total amount of the mixture; and the solid-liquid ratio of the mixture to pure water is 1:5-8.

[0009] Further, in step (4), the organic solvent A is a mixed solvent of ethyl acetate and ethanol in a volume ratio of 3-5:5; the solid-liquid ratio of the drug residue to organic solvent A is 1:5-8.

[0010] Further, in step (4), the organic solvent B is a mixed solvent of ethyl acetate and ethanol in a volume ratio of 1-3:4-7; the solid-liquid ratio of the residue to organic solvent B is 1:3-5. This invention utilizes the characteristics that ethyl acetate can selectively extract lipid-soluble components, ethanol can selectively extract water-soluble components, and different concentrations and temperatures yield different components. First, a mixed solvent with a relatively low ethanol content is used for low-temperature extraction, which effectively protects heat-sensitive components. Then, a mixed solvent with a relatively high ethanol content is used for higher-temperature extraction, which can further extract other components. This maximizes the extraction of effective components from the raw material, improving efficacy and bioavailability.

[0011] Furthermore, in step (5), the temperature for vacuum concentration is 40-60℃, and the concentration is carried out until the density is 1-1.5 g / cm³. 3 scope.

[0012] Furthermore, in step (5), the three-stage drying process is as follows: first, vacuum belt drying for 0.5-1h, then vacuum microwave drying for 0.3-0.5h, and finally fluidized bed drying until the moisture content is below 5%. Compared with the problem that high-temperature spray drying can easily lead to the decomposition of heat-sensitive components, the present invention adopts a three-stage low-temperature drying process, which is to maximize the retention of active components while ensuring drying efficiency by controlling the moisture removal rate and temperature in stages.

[0013] Furthermore, the vacuum belt dryer operates at a temperature of 60°C, a pressure of -0.08 MPa, and a conveying speed of 0.5-0.8 m / min; the vacuum microwave dryer operates at a power of 3 kW, a temperature of 50°C, and a pressure of -0.09 MPa; and the fluidized bed dryer operates at a temperature of 40°C and an air volume of 800 m³ / min. 3 / h. This invention first employs vacuum belt drying, which lowers the boiling point of water under negative pressure to achieve rapid evaporation of water at 60°C; microwave drying causes high-frequency vibration of water to generate heat, lowers the boiling point under low pressure, and further efficiently dehydrates the water; finally, a fluidized bed gently removes residual surface moisture, and airflow collisions make the powder uniform and fine, eliminating the need for further pulverization.

[0014] Furthermore, the muscle-relaxing and blood-activating capsule comprises the following raw material components in parts by weight: 21.0 parts of safflower, 78.6 parts of prepared Cyperus rotundus, 104.8 parts of prepared Cibotium barometz, 52.5 parts of Periploca sepium, 78.6 parts of Trachelospermum jasminoides, 78.6 parts of Lycopodium clavatum, 78.6 parts of Lycopodium clavatum leaf, 104.8 parts of mistletoe, 78.6 parts of Spatholobus suberectus, and 13.1 parts of calcined natural copper.

[0015] The second objective of this invention is to provide a muscle-relaxing and blood-activating capsule prepared by the above-described method.

[0016] Compared with the prior art, the technical solution of the present invention has the following beneficial effects: This invention optimizes and improves the preparation method by using citric acid to aid dissolution and ultrasound-assisted extraction, which can effectively increase the dissolution rate of iron ions and improve bioavailability. By using a preparation process that combines compound enzyme extraction, extraction with mixed solvents at different temperatures, and a three-stage drying process, each effective component can be extracted in stages while ensuring that each component is not decomposed. The resulting muscle-relaxing and blood-activating capsules have high content of effective components and good bioavailability, which can further reduce the dosage and improve patient compliance. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Unless otherwise specified, specific conditions in the embodiments are performed under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, all concentrations mentioned in the present invention refer to mass concentrations. Reagents or instruments used without a specified manufacturer are all commercially available conventional products.

[0018] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0019] The prepared capsules are made according to the formula, with 1000 capsules made from 690g of raw materials, and each capsule weighing 0.35g.

[0020] Example 1 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the muscle-relaxing and blood-activating capsule comprises the following raw material components in parts by weight: 20 parts of safflower, 79 parts of prepared Cyperus rotundus, 103 parts of prepared Cibotium barometz, 54 parts of Periploca sepium, 77 parts of Trachelospermum jasminoides, 77 parts of Lycopodium clavatum, 79 parts of Lycopodium clavatum leaf, 106 parts of mistletoe, 77 parts of Spatholobus suberectus, and 14 parts of calcined natural copper. The preparation method includes the following steps: (1) Prepare the above-mentioned raw materials and set aside; (2) After pulverizing the calcined natural copper, pass it through a 200-mesh sieve. Take the amount of pulverized calcined natural copper according to the formula, add a 1% citric acid solution (solid-liquid ratio 1:10), mix, and extract under ultrasonic conditions (300W, 70℃) for 2 hours. Filter, extract the filter residue once, combine the filtrates, and obtain filtrate a for later use. (3) Safflower, prepared Cyperus rotundus, prepared Cibotium barometz, Cibotium barometz bark, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaves, Mistletoe, and Spatholobus suberectus are pulverized and passed through a 100-mesh sieve. They are mixed according to the formula to obtain a mixture. Then, pure water (solid-liquid ratio 1:5) and a compound enzyme preparation (cellulase and pectinase mass ratio 1:1, usage 0.5%) are added. After mixing for 2 hours, the mixture is heated to 80℃ and kept warm for 10 minutes. After centrifugation, filtrate b and residue are obtained. (4) Add organic solvent A (ethyl acetate and ethanol volume ratio 3:5, solid-liquid ratio 1:5) to the residue from step (3), extract at 50℃ for 1h, filter to obtain filtrate c, add organic solvent B (ethyl acetate and ethanol volume ratio 1:4, solid-liquid ratio 1:3) to the residue, extract at 80℃ for 2h, filter under pressure to obtain filtrate d; (5) After mixing filtrates a, b, c, and d, concentrate them under reduced pressure at 40°C to a density of 1-1.5 g / cm³. 3 Then, a three-stage drying process is performed: first, vacuum belt drying for 0.5 hours at 60℃, -0.08MPa, and 0.5m / min; then, vacuum microwave drying for 0.3 hours at 3kW, 50℃, and -0.09MPa; and finally, drying at 40℃ and 800m³ / min. 3 Dry the capsules in a fluidized bed at a rate of / h until the moisture content is below 5%, then sieve and fill them into capsules to obtain the muscle-relaxing and blood-activating capsules.

[0021] Example 2 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the muscle-relaxing and blood-activating capsule comprises the following raw material components in parts by weight: 21 parts safflower, 78.6 parts prepared Cyperus rotundus, 104.8 parts prepared Cibotium barometz, 52.5 parts Periploca sepium, 78.6 parts Trachelospermum jasminoides, 78.6 parts Lycopodium clavatum, 78.6 parts Lycopodium clavatum leaf, 104.8 parts mistletoe, 78.6 parts Spatholobus suberectus, and 13.1 parts calcined natural copper; The preparation method includes the following steps: (1) Prepare the above-mentioned raw materials and set aside; (2) After pulverizing the calcined natural copper, pass it through a 200-mesh sieve. Take the amount of pulverized calcined natural copper according to the formula, add a 1% citric acid solution (solid-liquid ratio 1:12), mix, and extract under ultrasonic conditions (300W, 75℃) for 1.5h. Filter, extract the filter residue once, combine the filtrates, and obtain filtrate a for later use. (3) Safflower, prepared Cyperus rotundus, prepared Cibotium barometz, Cibotium barometz bark, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaves, Mistletoe, and Spatholobus suberectus are pulverized and passed through a 100-mesh sieve. They are then mixed according to the formula to obtain a mixture. Pure water (solid-liquid ratio 1:7) and a compound enzyme preparation (cellulase and pectinase mass ratio 1.5:1, usage 0.8%) are added. After mixing for 1.5 hours, the mixture is heated to 80°C and kept warm for 10 minutes. After centrifugation, filtrate b and residue are obtained. (4) Add organic solvent A (ethyl acetate and ethanol volume ratio 5:5, solid-liquid ratio 1:7) to the residue from step (3), extract at 50℃ for 1h, filter to obtain filtrate c, add organic solvent B (ethyl acetate and ethanol volume ratio 3:7, solid-liquid ratio 1:5) to the residue, extract at 80℃ for 1.5h, filter under pressure to obtain filtrate d; (5) After mixing filtrates a, b, c, and d, concentrate them under reduced pressure at 50°C to a density of 1-1.5 g / cm³. 3 Then, a three-stage drying process is performed: first, vacuum belt drying for 0.8 hours at 60℃, -0.08MPa, and 0.6m / min; then, vacuum microwave drying for 0.4 hours at 50℃ and -0.09MPa; and finally, drying at 40℃ and 800m³ / min. 3 Dry the capsules in a fluidized bed at a rate of / h until the moisture content is below 5%, then sieve and fill them into capsules to obtain the muscle-relaxing and blood-activating capsules.

[0022] Example 3 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the muscle-relaxing and blood-activating capsule comprises the following raw material components in parts by weight: 22 parts of safflower, 77 parts of prepared Cyperus rotundus, 106 parts of prepared Cibotium barometz, 51 parts of Periploca sepium, 79 parts of Trachelospermum jasminoides, 79 parts of Lycopodium clavatum, 77 parts of Lycopodium clavatum leaf, 103 parts of mistletoe, 79 parts of Spatholobus suberectus, and 12 parts of calcined natural copper. The preparation method includes the following steps: (1) Prepare the above-mentioned raw materials and set aside; (2) After pulverizing the calcined natural copper, pass it through a 200-mesh sieve. Take the amount of pulverized calcined natural copper according to the formula, add a 1% citric acid solution (solid-liquid ratio 1:15), mix, and extract under ultrasonic conditions (300W, 80℃) for 1 hour. Filter, extract the filter residue once, combine the filtrates, and obtain filtrate a for later use. (3) The safflower, prepared Cyperus rotundus, prepared Cibotium barometz, Cibotium barometz bark, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaves, Mistletoe, and Spatholobus suberectus are pulverized and passed through a 100-mesh sieve. They are then mixed according to the formula to obtain a mixture. Pure water (solid-liquid ratio 1:8) and a compound enzyme preparation (cellulase and pectinase mass ratio 2:1, usage 1%) are added. After mixing for 1 hour, the mixture is heated to 80℃ and kept warm for 10 minutes. After centrifugation, filtrate b and the residue are obtained. (4) Add organic solvent A (ethyl acetate and ethanol volume ratio 4:5, solid-liquid ratio 1:8) to the residue from step (3), extract at 50℃ for 0.5h, filter to obtain filtrate c, add organic solvent B (ethyl acetate and ethanol volume ratio 2:5, solid-liquid ratio 1:4) to the residue, extract at 80℃ for 1h, filter under pressure to obtain filtrate d; (5) After mixing filtrates a, b, c, and d, concentrate them under reduced pressure at 40-60℃ to a density of 1-1.5 g / cm³. 3 Then, a three-stage drying process is performed: first, vacuum belt drying for 1 hour at 60℃, -0.08MPa, and a conveyor speed of 0.8m / min; then, vacuum microwave drying for 0.5 hours at 3kW, 50℃, and -0.09MPa; and finally, drying at 40℃ and an air volume of 800m³ / min. 3 Dry the capsules in a fluidized bed at a rate of / h until the moisture content is below 5%, then sieve and fill them into capsules to obtain the muscle-relaxing and blood-activating capsules.

[0023] Comparative Example 1 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the raw material components are the same as those in Example 2.

[0024] The difference lies in the preparation method step (2): after pulverizing the calcined natural copper, it is passed through a 200-mesh sieve, water (solid-liquid ratio 1:12) is added and boiled for 2 hours, filtered, and the filtrate is used for later use.

[0025] Comparative Example 2 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the raw material components are the same as those in Example 2.

[0026] Its preparation method includes the following steps: (1) Prepare the above-mentioned raw materials and set aside; (2) After pulverizing the calcined natural copper, pass it through a 200-mesh sieve. Take the amount of pulverized calcined natural copper according to the formula, add a 1% citric acid solution (solid-liquid ratio 1:12), mix, and extract under ultrasonic conditions (300W, 75℃) for 1.5h. Filter, extract the filter residue once, combine the filtrates, and obtain filtrate a for later use. (3) Safflower, prepared Cyperus rotundus, prepared Cibotium barometz, Cibotium barometz bark, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaves, Mistletoe, and Spatholobus suberectus are pulverized and passed through a 100-mesh sieve. They are mixed according to the formula to obtain a mixture. Organic solvent A (ethyl acetate and ethanol volume ratio 5:5, solid-liquid ratio 1:7) is added and extracted at 50℃ for 1h. After filtration, filtrate c is obtained. The residue is further added to organic solvent B (ethyl acetate and ethanol volume ratio 3:7, solid-liquid ratio 1:5) and extracted at 80℃ for 1.5h. After pressure filtration, filtrate d is obtained. (4) After mixing filtrates a, c, and d, concentrate them under reduced pressure at 60°C to a density of 1-1.5 g / cm³. 3Then, a three-stage drying process is performed: first, vacuum belt drying for 0.8 hours at 60℃, -0.08MPa, and 0.6m / min; then, vacuum microwave drying for 0.4 hours at 50℃ and -0.09MPa; and finally, drying at 40℃ and 800m³ / min. 3 Dry the capsules in a fluidized bed at a rate of / h until the moisture content is below 5%, then sieve and fill them into capsules to obtain the muscle-relaxing and blood-activating capsules.

[0027] Comparative Example 3 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the raw material components are the same as those in Example 2.

[0028] The difference lies in the preparation method step (4): add organic solvent A (ethyl acetate and ethanol volume ratio 5:5, solid-liquid ratio 1:7) to the residue in step (3), extract at 50℃ for 2h, filter, and obtain filtrate c.

[0029] Comparative Example 4 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the raw material components are the same as those in Example 2.

[0030] The difference lies in the preparation method step (4): add organic solvent B (ethyl acetate and ethanol volume ratio 3:7, solid-liquid ratio 1:5) to the residue in step (3), extract at 80℃ for 1.5h, filter under pressure, and obtain filtrate d.

[0031] Comparative Example 5 A method for preparing a muscle-relaxing and blood-activating capsule, wherein the raw material components are the same as those in Example 2.

[0032] The difference lies in the preparation method step (5): after mixing filtrates a, b, c, and d, the mixture is concentrated under reduced pressure at 50°C to a density of 1-1.5 g / cm³. 3 Then, spray drying is carried out with an air inlet temperature of 170℃ and an air outlet temperature of 95℃. The powder is then used to fill capsules to obtain muscle-relaxing and blood-activating capsules.

[0033] Experimental Example 1 The effective components of the muscle-relaxing and blood-activating capsules obtained in Examples 1-3 and Comparative Examples 1-5, namely hydroxysaffron yellow A and trachelospermum glycoside, disintegration time, and bioavailability, were detected, and the results are shown in Tables 1 and 2, respectively. The detection methods for hydroxysaffron yellow A and trachelospermum glycoside were based on the relevant methods in Patent 201110129493.5; the disintegration time was based on the relevant methods for capsules in the 2020 edition of the Chinese Pharmacopoeia; the bioavailability was determined by measuring the blood concentration of hydroxysaffron yellow A in rats and calculated, based on the relevant methods in the 2020 edition of the Chinese Pharmacopoeia; calcined natural copper was administered via gavage using a rat model isotope tracing method. 59 Fe labeling was used to determine and calculate the radioactivity of whole blood.

[0034] Table 1. Results of tests on hydroxysaffron yellow A, trachelospermum erythrorhizon, and disintegration time.

[0035] Table 2 Pharmacokinetic parameters of hydroxysaffron yellow A

[0036] Table 3. Pharmacokinetic parameters of iron ions

[0037] As can be seen from the results in Tables 1-3, the muscle-relaxing and blood-activating capsules prepared by the method of the present invention have high content of effective ingredients, short disintegration time, and high bioavailability. In contrast, the iron ion bioavailability of Comparative Example 1 is relatively low due to the use of conventional water extraction to calcify natural copper. In Comparative Examples 2-4, the different extraction methods all affect the content of effective ingredients and bioavailability to some extent. In Comparative Example 5, spray drying technology is used, but the high temperature will destroy heat-sensitive components, resulting in a certain decrease in overall performance.

[0038] In summary, by optimizing the preparation method, the present invention produces muscle-relaxing and blood-activating capsules with high content of effective ingredients, short disintegration time, and high bioavailability. By reducing the dosage, such as three times a day or two capsules each time, patient compliance can be greatly improved without affecting the efficacy.

[0039] Finally, it should be noted that the above examples are merely some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments and many variations are possible. All variations that can be directly derived or conceived by those skilled in the art from the disclosure of the present invention should be considered within the scope of protection of the present invention.

Claims

1. A method for preparing a muscle-relaxing and blood-activating capsule, characterized in that, The muscle-relaxing and blood-activating capsules comprise the following raw material components in parts by weight: 20-22 parts safflower, 77-79 parts prepared Cyperus rotundus, 103-106 parts prepared Cibotium barometz, 51-54 parts Periploca sepium, 77-79 parts Trachelospermum jasminoides, 77-79 parts Lycopodium clavatum, 77-79 parts Lycopus lucidus leaf, 103-106 parts mistletoe, 77-79 parts Spatholobus suberectus, and 12-14 parts calcined natural copper; The preparation method includes the following steps: (1) Prepare the above-mentioned raw materials and set aside; (2) After pulverizing the calcined natural copper, pass it through a 200-mesh sieve. Take the amount of pulverized calcined natural copper according to the formula, add a 1% citric acid solution, mix, and extract under ultrasonic conditions for 1-2 hours. Filter, extract the filter residue once, combine the filtrates, and obtain filtrate a for later use. (3) The safflower, prepared Cyperus rotundus, prepared Cibotium barometz, Cibotium barometz bark, Trachelospermum jasminoides, Lycopodium clavatum, Lycopodium clavatum leaves, Mistletoe, and Spatholobus suberectus are pulverized and passed through a 100-mesh sieve. They are then mixed according to the formula to obtain a mixture. Pure water and compound enzyme preparation are added and mixed for 1-2 hours. The mixture is then heated to 80°C and kept warm for 10 minutes. After centrifugation, filtrate b and dregs are obtained. (4) Add organic solvent A to the residue from step (3), extract at 50°C for 0.5-1h, filter to obtain filtrate c, add organic solvent B to the residue, extract at 80°C for 1-2h, filter under pressure to obtain filtrate d; (5) After mixing the filtrates a, b, c and d, the mixture is concentrated under reduced pressure, then dried in three stages, sieved and filled into capsules to obtain the muscle-relaxing and blood-activating capsules.

2. The preparation method according to claim 1, characterized in that, In step (2), the solid-liquid ratio of calcined natural copper and citric acid solution is 1:10-15, and the ultrasonic conditions are 300W and 70-80℃.

3. The preparation method according to claim 1, characterized in that, In step (3), the compound enzyme preparation is a mixture of cellulase and pectinase in a mass ratio of 1-2:1; the amount of the compound enzyme preparation added is 0.5-1% of the total amount of the mixture; and the solid-liquid ratio of the mixture to pure water is 1:5-8.

4. The preparation method according to claim 1, characterized in that, In step (4), the organic solvent A is a mixed solvent of ethyl acetate and ethanol in a volume ratio of 3-5:5; the solid-liquid ratio of the drug residue to organic solvent A is 1:5-8.

5. The preparation method according to claim 1, characterized in that, In step (4), the organic solvent B is a mixed solvent of ethyl acetate and ethanol in a volume ratio of 1-3:4-7; the solid-liquid ratio of the residue to the organic solvent B is 1:3-5.

6. The preparation method according to claim 1, characterized in that, In step (5), the vacuum concentration temperature is 40-60℃, and the concentration is carried out until the density is 1-1.5 g / cm³. 3 scope.

7. The preparation method according to claim 1, characterized in that, In step (5), the three-stage drying process is as follows: first, vacuum belt drying for 0.5-1h, then vacuum microwave drying for 0.3-0.5h, and finally fluidized bed drying until the moisture content is below 5%.

8. The preparation method according to claim 7, characterized in that, The vacuum belt dryer operates at a temperature of 60℃, a pressure of -0.08MPa, and a conveying speed of 0.5-0.8m / min; the vacuum microwave dryer operates at a power of 3kW, a temperature of 50℃, and a pressure of -0.09MPa; the fluidized bed dryer operates at a temperature of 40℃ and an air volume of 800m³ / min. 3 / h.

9. The preparation method according to claim 1, characterized in that, The muscle-relaxing and blood-activating capsules comprise the following raw material components in parts by weight: 21.0 parts safflower, 78.6 parts prepared Cyperus rotundus, 104.8 parts prepared Cibotium barometz, 52.5 parts Periploca sepium, 78.6 parts Trachelospermum jasminoides, 78.6 parts Lycopodium clavatum, 78.6 parts Lycopus lucidus, 104.8 parts mistletoe, 78.6 parts Spatholobus suberectus, and 13.1 parts calcined natural copper.

10. A muscle-relaxing and blood-activating capsule prepared by the method according to any one of claims 1-9.

Images