Medical dressing and preparation method thereof

Abstract

The invention discloses a medical dressing and a preparation method thereof. The dressing is prepared from the following components in parts by weight: 30 to 100 parts of sodium carboxymethyl cellulose, 30 to 50 parts of polyethylene glycol, 30 to 50 parts of sodium alginate, 20 to 30 parts of sodium hyaluronate, 5 to 15 parts of a humectant, 3 to 8 parts of a plant extract, 2 to 6 parts of compound polysaccharide, 0.01 to 0.5 part of a growth factor, 0.2 to 0.6 part of balanophora balanophora, 0.1 to 0.5 part of ginsenoside Rg1 and 300 to 600 parts of deionized water. The medical dressing disclosed by the invention is good in antibacterial and anti-inflammatory effects and has a certain repairing effect on scars. The termitomyces albuminosus extract and the callicarpa bodinieri extract in the components are combined for use, so that the antibacterial effect is enhanced; the rosa roxburghii tratt polysaccharides and the aloe polysaccharides are combined to resist inflammation and promote wound healing; balanineine and ginsenoside Rg1 are combined for use to inhibit scar generation.

Description

Technical Field

[0001] This invention belongs to the field of medical materials technology, specifically relating to a medical dressing and its preparation method. Background Technology

[0002] Medical dressings are a class of medical materials used to cover wounds, absorb exudate, promote healing, or protect the wound surface. They are widely used in clinical nursing and home healthcare. The main functions of medical dressings include: protecting the wound surface and preventing bacterial infection and external contamination; absorbing exudate and maintaining wound moisture balance; promoting healing by providing a suitable environment; reducing pain by minimizing adhesion damage during dressing changes; and providing adjunctive therapy. Some dressings contain medication.

[0003] Most dressings have poor antibacterial properties, effective only against specific bacteria and unable to cover all common pathogens. Prolonged or improper use of antibacterial dressings (especially antibiotics) may induce bacterial resistance, increasing the difficulty of subsequent treatment and even triggering outbreaks of drug-resistant bacterial infections. Traditional antibacterial dressings lack sufficient penetration into existing bacterial biofilms (such as those formed by Pseudomonas aeruginosa), making it difficult to completely kill deep-seated bacteria, leading to recurrent infections and stunted healing of chronic wounds.

[0004] The application of dressings in scar management has certain limitations, especially in inhibiting scar hyperplasia and improving scar appearance. Scar dressings (such as silicone patches and pressure dressings) are mainly effective in the early stages of wound healing (within 1-3 months). Once the scar matures (fibrosis, hardening), the effect decreases significantly. Silicone dressings can partially inhibit mild hyperplasia, but their effect on severe hypertrophic scars or keloids (such as post-burn scars, keloids on the chest and back) is limited. They cannot inhibit excessive fibroblast proliferation and the continuous activation of the TGF-β signaling pathway. Dressings mainly inhibit hyperplasia, but are ineffective against pigmentation (erythema, melasma) and atrophic scars (such as acne pits).

[0005] Most anti-inflammatory dressings target only certain inflammatory factors (such as TNF-α and IL-6), but wound inflammation involves multiple cell types (macrophages, neutrophils) and signaling pathways (NF-κB, JAK / STAT). A single component cannot comprehensively regulate these factors; it may suppress some inflammatory responses but cannot thoroughly optimize the inflammatory microenvironment. Inflammation is a necessary stage for wound healing; excessive suppression may delay the conversion of macrophages to a repair phenotype, affecting granulation tissue formation and leading to delayed healing. Natural anti-inflammatory components (such as aloe vera and curcumin) are easily oxidized and rendered ineffective. Synthetic anti-inflammatory drugs (such as NSAIDs) may cause excessively high local concentrations due to burst release, irritating the wound. Anti-inflammatory effects are unstable, potentially effective in the short term but ineffective in the long term. Summary of the Invention

[0006] The present invention provides a medical dressing and a method for preparing the same.

[0007] A medical dressing comprises the following components in parts by weight: 30-100 parts sodium carboxymethyl cellulose, 30-50 parts polyethylene glycol, 30-50 parts sodium alginate, 20-30 parts sodium hyaluronate, 5-15 parts moisturizer, 3-8 parts plant extract, 2-6 parts complex polysaccharide, 0.01-0.5 parts growth factor, 0.2-0.6 parts ginsenoside Rg1, 0.1-0.5 parts ginsenoside Rg1, and 300-600 parts deionized water.

[0008] The plant extract is a mixture of termite mushroom extract and purple callicarpa extract in a mass ratio of 3:1.

[0009] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through an 80-120 mesh sieve, add 5-15 times the volume of 50-70% ethanol and reflux extract 1-3 times, each reflux extraction for 1-3 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0010] The extraction method of the purple pearl grass extract is as follows: take the dried termite mushroom, grind it into powder, pass it through an 80-120 mesh sieve, add 5-10 times the volume of 60-80% ethanol for reflux extraction 1-3 times, each reflux extraction for 1-3 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain purple pearl grass extract.

[0011] The complex polysaccharide is a mixture of prickly pear polysaccharide and aloe vera polysaccharide in a mass ratio of 1:2.

[0012] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 3-6 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 60-80℃ for 2-4 hours, centrifuged and dried; 10-20 times the mass of water is added, and the mixture is extracted at 80-100℃ for 2-4 hours, centrifuged, the filtrate is concentrated under reduced pressure, and precipitated with anhydrous ethanol to make the ethanol concentration 75-85%; finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found, and after removing impurities, the mixture is dialyzed and freeze-dried to obtain prickly pear polysaccharide.

[0013] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:(1-3). After stirring at room temperature for 200-300 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0014] The humectant is glycerin, 1,2-propanediol, or 1,3-propanediol.

[0015] The growth factor is one or more of epidermal growth factor, transforming growth factor-α, β-cytokinin, and fibroblast growth factor-1.

[0016] The preparation method of the medical dressing is carried out according to the following steps:

[0017] (1) Take 30-100 parts of sodium carboxymethyl cellulose, 30-50 parts of polyethylene glycol, 30-50 parts of sodium alginate, 20-30 parts of sodium hyaluronate, and 5-15 parts of humectant according to the weight ratio, add 300-600 parts of deionized water, heat to 50-60℃, and stir at 50-100 rpm for 20-30 minutes.

[0018] (2) Cool down to 20-30℃, add 3-8 parts of plant extract, 2-6 parts of complex polysaccharide, 0.01-0.5 parts of growth factor, 0.2-0.6 parts of snake ginseng, and 0.1-0.5 parts of ginsenoside Rg1, and stir at 30-50 rpm for 10-20 min;

[0019] (3) Sterilize by gamma ray irradiation or electron beam irradiation.

[0020] The beneficial effects of this invention are as follows: The medical dressing of this invention has good antibacterial and anti-inflammatory effects, and has a certain repair effect on scars. The combination of Termitomyces albuminosus extract and Callicarpa japonica extract in the components enhances the antibacterial effect; the combination of Prickly pear polysaccharide and Aloe polysaccharide has anti-inflammatory effects and promotes wound healing; and the combination of Scutellaria baicalensis extract and Ginsenoside Rg1 inhibits scar formation. Detailed Implementation

[0021] To facilitate understanding of the present invention, a more comprehensive description will be given below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.

[0022] Example 1

[0023] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts plant extract, 4 parts complex polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.4 parts ginsenoside Rg1, 0.3 parts ginsenoside Rg1, and 450 parts deionized water; wherein the plant extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1; and the complex polysaccharide is a mixture of Prickly pear polysaccharide and Aloe vera polysaccharide in a mass ratio of 1:2.

[0024] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 100-mesh sieve, add 10 times the volume of 60% ethanol and reflux extract twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0025] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through a 100-mesh sieve, add 8 times the volume of 70% ethanol and reflux extract it twice, each time for 2 hours of reflux extraction, filter it, combine the filtrates, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0026] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 70°C for 3 hours. After centrifugation and drying, 15 times the mass of water is added, and the mixture is extracted at 90°C for 3 hours. After centrifugation, the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 80%. Finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found. After removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

[0027] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:2. After stirring at room temperature for 250 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0028] The preparation method of the medical dressing is carried out according to the following steps:

[0029] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0030] (2) Cool down to 25℃, add 6 parts of plant extract, 4 parts of complex polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, 0.4 parts of snake ginseng, and 0.3 parts of ginsenoside Rg1, and stir at 40 rpm for 15 min.

[0031] (3) Sterilize by γ-ray irradiation to obtain the product.

[0032] Example 2

[0033] A medical dressing comprises the following components in parts by weight: 45 parts sodium carboxymethyl cellulose, 32 parts polyethylene glycol, 30 parts sodium alginate, 20 parts sodium hyaluronate, 5 parts 1,2-propanediol, 4 parts plant extract, 3 parts complex polysaccharide, 0.01 parts transforming growth factor-α, 0.01 parts β-cytokine, 0.2 parts ginsenoside Rg1, 0.1 parts ginsenoside Rg1, and 320 parts deionized water; wherein the plant extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1; and the complex polysaccharide is a mixture of Prickly pear polysaccharide and Aloe vera polysaccharide in a mass ratio of 1:2.

[0034] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through an 80-mesh sieve, add 6 times the volume of 70% ethanol and reflux to extract once, extract for 3 hours, filter, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0035] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through an 80-mesh sieve, add 6 times the volume of 80% ethanol and reflux to extract it once, extract for 3 hours, filter it, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0036] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 4 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 60°C for 4 hours, centrifuged and dried; 10 times the mass of water is added, and the mixture is extracted at 80°C for 4 hours, centrifuged, the filtrate is concentrated under reduced pressure, and precipitated with anhydrous ethanol to make the ethanol concentration 75%; finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found, and after removing impurities, the mixture is dialyzed and freeze-dried to obtain prickly pear polysaccharide.

[0037] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:1. After stirring at room temperature for 300 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0038] The preparation method of the medical dressing is carried out according to the following steps:

[0039] (1) Take 45 parts of sodium carboxymethyl cellulose, 32 parts of polyethylene glycol, 30 parts of sodium alginate, 20 parts of sodium hyaluronate, and 5 parts of 1,2-propanediol according to the weight ratio, add 320 parts of deionized water, heat to 50℃, and stir at 50 rpm for 30 min.

[0040] (2) Cool down to 20℃, add 4 parts of plant extract, 3 parts of complex polysaccharide, 0.01 parts of transforming growth factor-α, 0.01 parts of β-cytokine, 0.2 parts of snake worm rin, and 0.1 parts of ginsenoside Rg1, and stir at 30 rpm for 20 min.

[0041] (3) Sterilize by γ-ray irradiation to obtain the product.

[0042] Example 3

[0043] A medical dressing comprises the following components in parts by weight: 90 parts sodium carboxymethyl cellulose, 50 parts polyethylene glycol, 45 parts sodium alginate, 30 parts sodium hyaluronate, 15 parts 1,3-propanediol, 8 parts plant extract, 6 parts complex polysaccharide, 0.2 parts fibroblast growth factor-1, 0.6 parts ginseng extract, 0.4 parts ginsenoside Rg1, and 550 parts deionized water; wherein the herbal extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1; and the complex polysaccharide is a mixture of Prickly pear polysaccharide and Aloe vera polysaccharide in a mass ratio of 1:2.

[0044] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 120-mesh sieve, add 12 times the volume of 50% ethanol and reflux extract three times, each time for 3 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0045] The extraction method of the purple pearl grass extract is as follows: take the dried termite mushroom, grind it into powder, pass it through a 120-mesh sieve, add 10 times the volume of 60% ethanol for reflux extraction 3 times, each reflux extraction for 3 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain purple pearl grass extract.

[0046] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 80°C for 2 hours, centrifuged, and dried; 20 times the mass of water is added, and the mixture is extracted at 100°C for 2 hours, centrifuged, and the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 85%; finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found, and after removing impurities, the mixture is dialyzed and freeze-dried to obtain prickly pear polysaccharide.

[0047] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:(1-3). After stirring at room temperature for 200-300 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0048] The preparation method of the medical dressing is carried out according to the following steps:

[0049] (1) Take 90 parts of sodium carboxymethyl cellulose, 50 parts of polyethylene glycol, 45 parts of sodium alginate, 30 parts of sodium hyaluronate, and 15 parts of 1,3-propanediol according to the weight ratio, add 550 parts of deionized water, heat to 60℃, and stir at 100 rpm for 20 min.

[0050] (2) Cool down to 30℃, add 8 parts of plant extract, 6 parts of complex polysaccharide, 0.2 parts of fibroblast growth factor-1, 0.6 parts of snake gallin, and 0.4 parts of ginsenoside Rg1, and stir at 50 rpm for 10 min;

[0051] (3) Sterilize by electron beam irradiation to obtain the product.

[0052] Comparative Example 1

[0053] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts Termitomyces albuminosus extract, 4 parts complex polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.4 parts strychnine, 0.3 parts ginsenoside Rg1, and 450 parts deionized water; wherein the complex polysaccharide is a mixture of prickly pear polysaccharide and aloe polysaccharide in a mass ratio of 1:2.

[0054] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 100-mesh sieve, add 10 times the volume of 60% ethanol and reflux extract twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0055] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 70°C for 3 hours. After centrifugation and drying, 15 times the mass of water is added, and the mixture is extracted at 90°C for 3 hours. After centrifugation, the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 80%. Finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found. After removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

[0056] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:2. After stirring at room temperature for 250 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0057] The preparation method of the medical dressing is carried out according to the following steps:

[0058] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0059] (2) Cool down to 25℃, add 6 parts of Termitomyces extract, 4 parts of complex polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, 0.4 parts of snake ginseng, and 0.3 parts of ginsenoside Rg1, and stir at 40 rpm for 15 min.

[0060] (3) Sterilize by γ-ray irradiation to obtain the product.

[0061] Comparative Example 2

[0062] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts Callicarpa japonica extract, 4 parts complex polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.4 parts ginsenoside Rg1, 0.3 parts ginsenoside Rg1, and 450 parts deionized water; wherein the complex polysaccharide is a mixture of prickly pear polysaccharide and aloe polysaccharide in a mass ratio of 1:2.

[0063] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through a 100-mesh sieve, add 8 times the volume of 70% ethanol and reflux extract it twice, each time for 2 hours of reflux extraction, filter it, combine the filtrates, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0064] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 70°C for 3 hours. After centrifugation and drying, 15 times the mass of water is added, and the mixture is extracted at 90°C for 3 hours. After centrifugation, the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 80%. Finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found. After removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

[0065] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:2. After stirring at room temperature for 250 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0066] The preparation method of the medical dressing is carried out according to the following steps:

[0067] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0068] (2) Cool down to 25℃, add 6 parts of Callicarpa japonica extract, 4 parts of complex polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, 0.4 parts of ginsenoside Rg1, and stir at 40 rpm for 15 min.

[0069] (3) Sterilize by γ-ray irradiation to obtain the product.

[0070] Comparative Example 3

[0071] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts plant extract, 4 parts prickly pear polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.4 parts strychnine, 0.3 parts ginsenoside Rg1, and 450 parts deionized water; wherein the plant extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1.

[0072] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 100-mesh sieve, add 10 times the volume of 60% ethanol and reflux extract twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0073] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through a 100-mesh sieve, add 8 times the volume of 70% ethanol and reflux extract it twice, each time for 2 hours of reflux extraction, filter it, combine the filtrates, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0074] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 70°C for 3 hours. After centrifugation and drying, 15 times the mass of water is added, and the mixture is extracted at 90°C for 3 hours. After centrifugation, the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 80%. Finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found. After removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

[0075] The preparation method of the medical dressing is carried out according to the following steps:

[0076] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0077] (2) Cool down to 25℃, add 6 parts of plant extract, 4 parts of prickly pear polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, 0.4 parts of snake ginseng, and 0.3 parts of ginsenoside Rg1, and stir at 40 rpm for 15 min.

[0078] (3) Sterilize by γ-ray irradiation to obtain the product.

[0079] Comparative Example 4

[0080] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts plant extract, 4 parts aloe polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.4 parts ginsenoside Rg1, 0.3 parts ginsenoside Rg1, and 450 parts deionized water; wherein the plant extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1.

[0081] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 100-mesh sieve, add 10 times the volume of 60% ethanol and reflux extract twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0082] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through a 100-mesh sieve, add 8 times the volume of 70% ethanol and reflux extract it twice, each time for 2 hours of reflux extraction, filter it, combine the filtrates, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0083] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:2. After stirring at room temperature for 250 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0084] The preparation method of the medical dressing is carried out according to the following steps:

[0085] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0086] (2) Cool down to 25℃, add 6 parts of aloe polysaccharide, 4 parts of complex polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, 0.4 parts of snake ginseng, and 0.3 parts of ginsenoside Rg1, and stir at 40 rpm for 15 min.

[0087] (3) Sterilize by γ-ray irradiation to obtain the product.

[0088] Comparative Example 5

[0089] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts plant extract, 4 parts complex polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.7 parts strychnine, and 450 parts deionized water; wherein the plant extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1; and the complex polysaccharide is a mixture of Prickly pear polysaccharide and Aloe vera polysaccharide in a mass ratio of 1:2.

[0090] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 100-mesh sieve, add 10 times the volume of 60% ethanol and reflux extract twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0091] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through a 100-mesh sieve, add 8 times the volume of 70% ethanol and reflux extract it twice, each time for 2 hours of reflux extraction, filter it, combine the filtrates, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0092] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 70°C for 3 hours. After centrifugation and drying, 15 times the mass of water is added, and the mixture is extracted at 90°C for 3 hours. After centrifugation, the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 80%. Finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found. After removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

[0093] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:2. After stirring at room temperature for 250 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0094] The preparation method of the medical dressing is carried out according to the following steps:

[0095] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0096] (2) Cool down to 25℃, add 6 parts of plant extract, 4 parts of complex polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, and 0.7 parts of snake gallin, and stir for 15 minutes at 40 rpm.

[0097] (3) Sterilize by γ-ray irradiation to obtain the product.

[0098] Comparative Example 6

[0099] A medical dressing comprises the following components in parts by weight: 60 parts sodium carboxymethyl cellulose, 40 parts polyethylene glycol, 40 parts sodium alginate, 25 parts sodium hyaluronate, 10 parts glycerin, 6 parts plant extract, 4 parts complex polysaccharide, 0.03 parts epidermal growth factor, 0.03 parts transforming growth factor-α, 0.7 parts ginsenoside Rg1, and 450 parts deionized water; wherein the plant extract is a mixture of Termitomyces albuminosus extract and Callicarpa japonica extract in a mass ratio of 3:1; and the complex polysaccharide is a mixture of Prickly pear polysaccharide and Aloe vera polysaccharide in a mass ratio of 1:2.

[0100] The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through a 100-mesh sieve, add 10 times the volume of 60% ethanol and reflux extract twice, each time for 2 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

[0101] The extraction method of the purple pearl grass extract is as follows: take the termite mushroom, dry it in the sun, grind it into powder, pass it through a 100-mesh sieve, add 8 times the volume of 70% ethanol and reflux extract it twice, each time for 2 hours of reflux extraction, filter it, combine the filtrates, concentrate it under reduced pressure until dry to obtain the purple pearl grass extract.

[0102] The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 5 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 70°C for 3 hours. After centrifugation and drying, 15 times the mass of water is added, and the mixture is extracted at 90°C for 3 hours. After centrifugation, the filtrate is concentrated under reduced pressure and precipitated with anhydrous ethanol to make the ethanol concentration 80%. Finally, the protein is removed with Sevage reagent (chloroform: n-butanol volume ratio = 4:1) until no white precipitate is found. After removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

[0103] The preparation method of the aloe polysaccharide is as follows: 1:100 (concentration ratio: 1:100 means that 1 kg of freeze-dried powder is equivalent to the active ingredient of 100 kg of fresh aloe vera gel juice) of Aloe vera gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:2. After stirring at room temperature for 250 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

[0104] The preparation method of the medical dressing is carried out according to the following steps:

[0105] (1) Take 60 parts of sodium carboxymethyl cellulose, 40 parts of polyethylene glycol, 40 parts of sodium alginate, 25 parts of sodium hyaluronate, and 10 parts of glycerin according to the weight ratio, add 450 parts of deionized water, heat to 55℃, and stir for 25 minutes at 80 rpm.

[0106] (2) Cool down to 25℃, add 6 parts of plant extract, 4 parts of complex polysaccharide, 0.03 parts of epidermal growth factor, 0.03 parts of transforming growth factor-α, and 0.7 parts of ginsenoside Rg1, and stir at 40 rpm for 15 min.

[0107] (3) Sterilize by γ-ray irradiation to obtain the product.

[0108] Experimental Example 1

[0109] Activated Staphylococcus aureus and Pseudomonas aeruginosa were inoculated onto ordinary broth agar plates and incubated at 37°C for 24 hours. The bacterial cells were then diluted to a final concentration with nutrient broth. 8 CFU / mL was added to the medical dressings prepared in Examples 1-3 and Comparative Examples 1-2, and incubated at room temperature for 1 hour. 50 μL of each dressing was spread onto plates and incubated upside down at 37°C for 24 hours. Colony growth was observed and the number of colonies was counted. Sterile water was used as a blank control group. Three parallel experiments were conducted for each group. The inhibition rate was calculated as (total number of colonies in the blank control group - total number of colonies in the experimental group) / total number of colonies in the blank control group × 100%. The experimental results were statistically analyzed using SPSS 24.0 software. Quantitative data were analyzed using... The mean ± standard deviation is expressed as mean ± standard deviation. The Kolmogorov-Smirnov test was used to test the normality of the data. For data that conform to a normal distribution, the t-test was used to compare the differences in means between the two groups. P < 0.05 was considered statistically significant. The results are shown in Table 1.

[0110] Table 1

[0111]

[0112] Note: * indicates that compared with Example 1 group, P<0.05.

[0113] Experiment Example 2

[0114] Male Wistar rats, weighing 240-250g, were selected. Each group of rats was anesthetized with compound ketamine, and their backs were shaved and fed normally for 3 days. A circular wound with a diameter of 1.5cm was surgically cut. The day after the operation, each group of animals was coated with the medical dressings of Examples 1-3 and Comparative Examples 3-6 twice a day.

[0115] Routine observations of animal behavior and wound appearance were conducted daily, and wound area was measured every other day. Experimental results were statistically analyzed using SPSS 24.0 software. Quantitative data were analyzed using... The Kolmogorov-Smirnov test was used to test the normality of the data. For data that conform to a normal distribution, the t-test was used to compare the differences in means between the two groups. P < 0.05 was considered statistically significant. The results are shown in Table 2.

[0116] Table 2

[0117]

[0118] Note: * indicates that compared with Example 1 group, P<0.05.

[0119] The scar hyperplasia index was calculated for each group of rats. The scar hyperplasia index = vertical height from the highest point of the scar protrusion to the subcutaneous muscle tissue ÷ vertical height from the normal skin surface surrounding the scar to the subcutaneous muscle tissue. The experimental results were statistically analyzed using SPSS 24.0 software. Quantitative data were analyzed using... The Kolmogorov-Smirnov test was used to test the normality of the data. For data that conform to a normal distribution, the t-test was used to compare the differences in means between the two groups. A p-value < 0.05 was considered statistically significant. The results are shown in Table 3.

[0120] Table 3

[0121]

[0122]

[0123] Note: * indicates that compared with Example 1 group, P<0.05.

[0124] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A medical dressing, characterized in that, It includes the following components in parts by weight: sodium carboxymethyl cellulose 30-100 parts, polyethylene glycol 30-50 parts, sodium alginate 30-50 parts, sodium hyaluronate 20-30 parts, moisturizer 5-15 parts, plant extract 3-8 parts, complex polysaccharide 2-6 parts, growth factor 0.01-0.5 parts, ginsenoside Rg1 0.2-0.6 parts, and deionized water 300-600 parts.

2. The medical dressing according to claim 1, characterized in that, The plant extract is a mixture of termite mushroom extract and purple callicarpa extract in a mass ratio of 3:

1.

3. The medical dressing according to claim 1, characterized in that, The extraction method of the termite mushroom extract is as follows: take termite mushrooms, dry them in the sun, grind them into powder, pass them through an 80-120 mesh sieve, add 5-15 times the volume of 50-70% ethanol and reflux extract 1-3 times, each reflux extraction for 1-3 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain termite mushroom extract.

4. The medical dressing according to claim 1, characterized in that, The extraction method of the purple pearl grass extract is as follows: take the dried termite mushroom, grind it into powder, pass it through an 80-120 mesh sieve, add 5-10 times the volume of 60-80% ethanol for reflux extraction 1-3 times, each reflux extraction for 1-3 hours, filter, combine the filtrates, concentrate under reduced pressure until dry to obtain purple pearl grass extract.

5. The medical dressing according to claim 1, characterized in that, The complex polysaccharide is a mixture of prickly pear polysaccharide and aloe vera polysaccharide in a mass ratio of 1:

2.

6. The medical dressing according to claim 5, characterized in that, The preparation method of the prickly pear polysaccharide is as follows: Prickly pear is crushed, 3-6 times the mass of 95% ethanol is added, and the mixture is stirred and defatted at 60-80℃ for 2-4 hours, centrifuged and dried; 10-20 times the mass of water is added, and the mixture is extracted at 80-100℃ for 2-4 hours, centrifuged, the filtrate is concentrated under reduced pressure, and precipitated with anhydrous ethanol to make the ethanol concentration 75-85%; finally, the protein is removed with Sevage reagent until no white precipitate is found, and after removing impurities, the mixture is dialyzed and freeze-dried to obtain the prickly pear polysaccharide.

7. The medical dressing according to claim 5, characterized in that, The preparation method of the aloe polysaccharide is as follows: 1:100 Curacao aloe gel freeze-dried powder is added to distilled water at a water-to-powder mass ratio of 100:(1-3). After stirring at room temperature for 200-300 min, the mixture is loaded onto a Sephadex G-100 column, eluted with distilled water, collected, precipitated with 95% ethanol, and then separated using a DEAE-agarose gel FF column with distilled water as the eluent. After freeze-drying, aloe polysaccharide is obtained.

8. The medical dressing according to claim 1, characterized in that, The humectant is glycerin, 1,2-propanediol, or 1,3-propanediol.

9. The medical dressing according to claim 1, characterized in that, The growth factor is one or more of epidermal growth factor, transforming growth factor-α, β-cytokinin, and fibroblast growth factor-1.

10. The method for preparing the medical dressing according to claim 1, characterized in that, Follow these steps: (1) Take 30-100 parts of sodium carboxymethyl cellulose, 30-50 parts of polyethylene glycol, 30-50 parts of sodium alginate, 20-30 parts of sodium hyaluronate, and 5-15 parts of humectant according to the weight ratio, add 300-600 parts of deionized water, heat to 50-60℃, and stir at 50-100 rpm for 20-30 minutes. (2) Cool down to 20-30℃, add 3-8 parts of plant extract, 2-6 parts of complex polysaccharide, 0.01-0.5 parts of growth factor, 0.2-0.6 parts of snake ginseng, and 0.1-0.5 parts of ginsenoside Rg1, and stir at 30-50 rpm for 10-20 min; (3) Sterilize by gamma ray irradiation or electron beam irradiation.

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