Baijiang soil modifier based on microorganism-animal interaction network and preparation method of Baijiang soil modifier

Abstract

The invention belongs to the technical field of soil improvement, and discloses a microorganism-animal interaction network-based Baijiang soil improver, which comprises the following components: nitrogen-fixing microorganisms, phosphorus-dissolving microorganisms, potassium-dissolving microorganisms, soil animals, auxiliary microorganisms, carrier substances, nutrient substances and a conditioning agent. Compared with a traditional soil conditioner, the soil conditioner has the advantages that microorganisms and soil animals are added into the conditioner, so that a stable biological symbiotic ecological system is constructed, and the improvement of soil fertility and the improvement of plough layer properties are promoted by virtue of interaction between the soil animals and the microorganisms; meanwhile, a directed multilayer biological network analysis method is combined to construct a fertile soil multilayer biological network aiming at the Baijiang soil, key microbial nodes are identified to realize regulation and control on fertile soil microbial communities, and aiming at the characteristics that the Baijiang soil is poor in soil structure and low in available nutrient, multi-organism synergistic Baijiang soil improvement can be more efficiently realized.

Description

Technical Field

[0001] This invention belongs to the field of soil improvement technology, specifically a white soil conditioner based on a microbial-animal interaction network and its preparation method. Background Technology

[0002] Soil conditioners, also known as soil amendments, are substances that improve the physical, chemical, and biological properties of soil and promote nutrient absorption by crops. Based on their main components and mechanisms of action, they can be divided into: organic conditioners, which mainly include farmyard manure, green manure, humic acid fertilizers, bark, soybean cake, lignin, and biogas residue; biological conditioners, which include microbial agents and plant growth regulators; and compound conditioners, which are mixtures of organic, inorganic, and biological conditioners in a certain proportion, possessing multiple improvement effects.

[0003] Soil conditioners are essential for soil improvement and optimization, especially for albic soils. The acidic, hard, and infertile characteristics of albic soils hinder the downward growth and development of crop roots and make water, fertilizer, air, and heat exchange difficult. As a soil with a typical barrier layer, its low organic matter content can negatively impact crop growth and development. Existing soil conditioners mainly work by applying fertilizers or adding microbial agents. However, in practice, the excessive use of chemical fertilizers can easily pollute the environment and even damage the original ecosystem of albic soils, causing an imbalance. Furthermore, relying solely on chemical or biological conditioners results in low nutrient cycling and utilization rates in the soil, thus prolonging the improvement time for albic soils. Therefore, improvements are needed. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a microbial-animal interaction network-based albican soil conditioner and its preparation method, which has the advantages of maintaining ecological balance and accelerating the improvement efficiency.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a microbial-animal interaction network-based albic soil conditioner, comprising the following components: nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, auxiliary microorganisms, soil animals, carrier substances, nutrients, and regulators, wherein, by weight fraction:

[0006] The nitrogen-fixing microorganisms: 1-3 portions;

[0007] Phosphate-solubilizing microorganisms: 1-3 parts;

[0008] Potassium-solubilizing microorganisms: 1-3 parts;

[0009] The auxiliary microorganisms: 1-3 parts;

[0010] The soil animals: 6-12 parts;

[0011] The carrier substance: 10-20 parts;

[0012] The stated nutrients: 3-5 servings;

[0013] The regulator: 2-4 parts.

[0014] As a preferred embodiment of the present invention, the nitrogen-fixing microorganism is selected from *Azospirillum*, the phosphate-solubilizing microorganism is selected from *Pseudomonas*, which is a high-yield species of extracellular polysaccharides, the potassium-solubilizing microorganism is selected from silicate bacteria, the auxiliary microorganisms include putrefactive microorganisms, growth-promoting microorganisms, disease-resistant microorganisms and arbuscular mycorrhizal fungi, the putrefactive microorganisms are selected from *Bacillus amyloliquefaciens* and *Laurella vegetans*, the growth-promoting microorganisms are selected from *Bacillus belye* and *Bacillus subtilis*, and the disease-resistant microorganism is selected from *Streptomyces*.

[0015] As a preferred embodiment of the present invention, the soil animal is selected from one or more of earthworms, nematodes, and ants.

[0016] As a preferred embodiment of the present invention, the carrier material is a mixture of straw and earthworm castings, wherein the straw and earthworm castings are mixed in a ratio of 2:2 to form the carrier material.

[0017] As a preferred embodiment of the present invention, the nutrients include urea, superphosphate and potassium sulfate, and the urea, superphosphate and potassium sulfate are used as inorganic fertilizer in a ratio of 21:15:16.

[0018] As a preferred embodiment of the present invention, the regulator is selected from humic acid and biochar, and is applied according to the soil acidity or alkalinity. For acidic soil, it is used at a ratio of 1:2; for neutral soil, it is used at a ratio of 1:1; and for alkaline soil, it is used at a ratio of 2:1.

[0019] As a preferred embodiment of the present invention, the soil conditioner is applied evenly to the soil of corn, wheat, and vegetable gardens at a dosage of 100-150 kg per hectare.

[0020] As a preferred technical solution of the present invention, it mainly includes the following steps:

[0021] Step 1: Microbial sampling

[0022] Use a shovel to collect good quality soil, then mix and dissolve the collected soil in pure water to prepare a suspension, which allows microorganisms to be released and dispersed. Then filter the prepared suspension to remove impurities, and divide the processed suspension into four equal parts and put them into four different petri dishes.

[0023] Step 2: Enrichment Culture

[0024] Neutral enrichment nutrient solution and inhibitors that inhibit the growth of other microorganisms were added to four petri dishes respectively. The petri dishes were then placed at room temperature for one week to allow the microorganisms in the four petri dishes to multiply in large quantities.

[0025] Step 3: Purebred Isolation

[0026] The microbial suspensions in the four petri dishes were serially diluted and then inoculated into four different solid culture media. Then, the nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms and helper microorganisms in the solid culture media were isolated by dilution plating method, so that the individual microorganisms formed independent colonies on the culture media.

[0027] Step 4: Filtering

[0028] The growth rate, metabolites, and enzyme activities of different independent colonies were tested. Then, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and helper microorganisms with consistent morphology, clear edges, smooth surfaces, and good colony growth were screened and placed in slant culture medium and stored at room temperature.

[0029] Step 5: Collection of Soil Animals

[0030] Collect an appropriate amount of earthworms, then clean the collected earthworms to remove impurities and pathogens. Then select earthworms that are healthy, active, and similar in size and shape and put them into a culture tank and store them at room temperature.

[0031] Step Six: Raw Material Preparation

[0032] Select pure straw and earthworm castings, then mix them evenly in a 2:2 ratio. Next, add three inorganic fertilizers, namely urea, superphosphate and potassium sulfate, in a 21:15:16 ratio. At the same time, add two organic fertilizers, namely humic acid and amino acids, in a 1:1 ratio. Finally, mix them thoroughly to obtain the mixture.

[0033] Step 7: Soil amendment preparation

[0034] The pH value of the mixture was tested, and then fulvic acid and biochar were added according to the measured pH value. The ratio was 1:2 for acidic soil, 1:1 for neutral soil, and 2:1 for alkaline soil. By using fulvic acid and biochar as regulators, the pH value of the mixture was adjusted to maintain a neutral pH. Subsequently, soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms were added to the mixture in sequence. The mixture was then placed in a tank for cultivation at room temperature. At the same time, a multi-layer biological network analysis method was used to construct a soil fertility network for albic soil. Key microbial nodes were identified to regulate the soil microbial community, allowing soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms to reproduce fully. This ensured that soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms coexisted harmoniously, forming a stable microbial-animal symbiotic system. In this way, an albic soil conditioner based on a microbial-animal interaction network can be prepared.

[0035] Step 8: Packaging

[0036] The prepared soil conditioner is packaged in equal amounts into bags of the same size for easy use.

[0037] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0038] Compared to traditional soil conditioners, this invention constructs a stable symbiotic ecosystem by adding microorganisms and soil animals to the conditioner. It utilizes the interaction between soil animals and microorganisms to promote soil fertility improvement and topsoil properties. Simultaneously, it combines directed multilayer biological network analysis to construct a multilayer biological network for albic soil, identify key microbial nodes to regulate the albic soil microbial community, and achieve more efficient multi-biological synergistic albic soil improvement, addressing the characteristics of poor soil structure and low available nutrients. Detailed Implementation

[0039] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] In this embodiment of the invention, a microbial-animal interaction network-based albican soil conditioner comprises the following components: nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, auxiliary microorganisms, soil animals, carrier substances, nutrients, and regulators, wherein, by weight fraction:

[0041] Nitrogen-fixing microorganisms: 1-3 portions;

[0042] Phosphate-solubilizing microorganisms: 1-3 parts;

[0043] Potassium-solubilizing microorganisms: 1-3 portions;

[0044] Supporting microorganisms: 1-3 parts;

[0045] Soil animals: 6-12 parts;

[0046] Carrier substance: 10-20 parts;

[0047] Nutrients: 3-5 servings;

[0048] Regulator: 2-4 parts.

[0049] The presence of nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms will facilitate the decomposition and release of nitrogen, phosphorus, potassium, and other nutrients, thereby providing a suitable growth environment for soil animals. The presence of carrier materials increases soil porosity and aeration, which is conducive to the survival and activity of microorganisms and soil animals. A stable symbiotic cooperative relationship is formed between microorganisms and soil animals, thereby constructing a more complex and stable microbial-animal symbiotic ecosystem, accelerating the decomposition of organic matter and the release of nutrients, and thus improving the characteristics of albic soil that is hard and has low organic matter content, forming an efficient nutrient cycling system and improving the improvement efficiency of albic soil.

[0050] Among them, nitrogen-fixing microorganisms were selected from *Azospirillum*, phosphorus-solubilizing microorganisms were selected from *Pseudomonas* spp. (*Pseudomonas* spp. is a high-yield species of extracellular polysaccharides), potassium-solubilizing microorganisms were selected from silicate bacteria, and auxiliary microorganisms included putrefactive microorganisms, growth-promoting microorganisms, disease-resistant microorganisms, and arbuscular mycorrhizal fungi. Putrefactive microorganisms were selected from *Bacillus amyloliquefaciens* and *Laurella vegetans*, growth-promoting microorganisms were selected from *Bacillus belye* and *Bacillus subtilis*, and disease-resistant microorganisms were selected from *Streptomyces*.

[0051] Azospirillum has nitrogen-fixing capabilities, which increases the nitrogen content in the soil, providing more food sources for soil animals. The activity of these soil animals, in turn, helps the distribution and reproduction of free-living azospirillum in the soil. Pseudomonas, as one of the bacteria with phosphorus-solubilizing capabilities, can convert insoluble phosphorus into soluble phosphorus. This phosphorus-solubilizing activity improves phosphorus utilization in the soil, promotes the growth and reproduction of soil animals, and also promotes the formation of soil aggregates. Silicate bacteria can decompose silicate minerals, releasing potassium and other nutrients. This potassium-solubilizing activity increases the available potassium content in the soil, providing more potassium nutrition for soil animals. The activity of these soil animals also helps the spread and reproduction of silicate bacteria in the soil. Arbuscular mycorrhizal fungi can promote plant growth and development, improve plant performance, enhance plant resistance, and produce glomerulonephrine, which promotes the formation of aggregates, thereby improving soil structure. Straw-decomposing microorganisms can promote the decomposition of straw.

[0052] Among them, soil animals are selected from one or more of earthworms, nematodes, and ants.

[0053] Earthworms, nematodes, and ants, as soil animals, can turn over the soil, increasing its aeration and permeability. They can also promote the decomposition of organic matter and nutrient cycling through their feeding and excretion activities. The activities of soil animals can also improve soil structure, increase soil aggregates, and reduce soil compaction. At the same time, combined with the directed multilayer biological network analysis method, a fertile soil multilayer biological network for albic soil can be constructed. The directed multilayer biological network analysis method can take into account time factors and dynamic changes, providing a more comprehensive view of the biological system, thereby more accurately describing the dynamic behavior of the biological system. It helps to discover the interactions and potential connections between soil animals and nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms at different levels, thereby identifying key microbial nodes, realizing the regulation of the fertile soil microbial community, and ensuring that a stable and efficient symbiotic cooperative relationship can be formed between soil animals and nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms.

[0054] The carrier material is a mixture of straw and earthworm castings, which are mixed in a 2:2 ratio to form the carrier material.

[0055] Straw and earthworm castings can adsorb and fix microorganisms and soil animals, improve the stability and application effect of soil conditioners, provide a good living environment for microorganisms and soil animals, increase the porosity and aeration of soil conditioners, which is conducive to the growth and reproduction of microorganisms. At the same time, the activity of soil animals can also promote the decomposition of carrier materials, thereby releasing nutrients in the carrier materials.

[0056] The nutrients include urea, superphosphate, and potassium sulfate, which are used as inorganic fertilizers in a ratio of 21:15:16.

[0057] Urea is a long-acting nitrogen fertilizer, suitable for use when crops have a long growth cycle; superphosphate is a phosphate fertilizer with high phosphorus content, characterized by long-lasting effect and easy water solubility, which can promote the growth and development of plant roots; potassium sulfate also has the characteristics of long-lasting effect and easy water solubility, which can promote the growth and development of plant stems and enhance the plant's resistance to lodging.

[0058] Among them, the conditioning agents are humic acid and biochar, which are applied according to the soil acidity and alkalinity. For acidic soil, the ratio is 1:2; for neutral soil, the ratio is 1:1; and for alkaline soil, the ratio is 2:1.

[0059] Fulvic acid is a type of humic acid. Because biochar is alkaline and has a high biochar content, fulvic acid and biochar are used to adjust the pH, which can prevent acidic or alkaline environments from affecting the growth of microorganisms and soil animals.

[0060] The soil conditioner should be applied evenly to the soil of corn, wheat, and vegetable gardens at a rate of 100-150 kg per hectare.

[0061] The specific amount of soil conditioner to be used can be determined based on the actual conditions of the soil in corn, wheat, and vegetable gardens, and additional application can be made in the later stages.

[0062] A method for preparing a limestone amendment based on a microbial-animal interaction network mainly includes the following steps:

[0063] Step 1: Microbial sampling

[0064] Use a shovel to collect good quality soil, then mix and dissolve the collected soil in pure water to prepare a suspension, which allows microorganisms to be released and dispersed. Then filter the prepared suspension to remove impurities, and divide the processed suspension into four equal parts and put them into four different petri dishes.

[0065] Step 2: Enrichment Culture

[0066] Neutral enrichment nutrient solution and inhibitors that inhibit the growth of other microorganisms were added to four petri dishes respectively. The petri dishes were then placed at room temperature for one week to allow the microorganisms in the four petri dishes to multiply in large quantities.

[0067] Step 3: Purebred Isolation

[0068] The microbial suspensions in the four petri dishes were serially diluted and then inoculated into four different solid culture media. Then, the nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms and helper microorganisms in the solid culture media were isolated by dilution plating method, so that the individual microorganisms formed independent colonies on the culture media.

[0069] Step 4: Filtering

[0070] The growth rate, metabolites, and enzyme activities of different independent colonies were tested. Then, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and helper microorganisms with consistent morphology, clear edges, smooth surfaces, and good colony growth were screened and placed in slant culture medium and stored at room temperature.

[0071] Step 5: Collection of Soil Animals

[0072] Collect an appropriate amount of earthworms, then clean the collected earthworms to remove impurities and pathogens. Then select earthworms that are healthy, active, and similar in size and shape and put them into a culture tank and store them at room temperature.

[0073] Step Six: Raw Material Preparation

[0074] Select pure straw and earthworm castings, then mix them evenly in a 2:2 ratio. Next, add three inorganic fertilizers, namely urea, superphosphate and potassium sulfate, in a 21:15:16 ratio. At the same time, add two organic fertilizers, namely humic acid and amino acids, in a 1:1 ratio. Finally, mix them thoroughly to obtain the mixture.

[0075] Step 7: Soil Conditioner Preparation

[0076] The pH value of the mixture was tested, and then fulvic acid and biochar were added according to the measured pH value. The ratio was 1:2 for acidic soil, 1:1 for neutral soil, and 2:1 for alkaline soil. By using fulvic acid and biochar as regulators, the pH value of the mixture was adjusted to maintain a neutral pH. Subsequently, soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms were added to the mixture in sequence. The mixture was then placed in a tank for cultivation at room temperature. At the same time, a multi-layer biological network analysis method was used to construct a soil fertility network for albic soil. Key microbial nodes were identified to regulate the soil microbial community, allowing soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms to reproduce fully. This ensured that soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms coexisted harmoniously, forming a stable microbial-animal symbiotic system. In this way, an albic soil conditioner based on a microbial-animal interaction network can be prepared.

[0077] Step 8: Packaging

[0078] The prepared soil conditioner is packaged in equal amounts into bags of the same size for easy use.

[0079] When sampling microorganisms, sterile tools should be used to ensure that the samples are not contaminated during the collection process. At the same time, during the preparation process, it is necessary to ensure that the activity of microorganisms and soil animals is not damaged and that all components are mixed evenly.

[0080] Example 1

[0081] Prepare the following three types of albic soil conditioners (low-dose, medium-dose, and high-dose) as shown in the table below, following the steps described above:

[0082]

[0083]

[0084] Application Example 1

[0085] The effect of the soil conditioner prepared in Example 1 on improving albic soil in paddy fields was determined by a greenhouse pot experiment:

[0086] The test soil was alkaline soil from paddy fields in Tongjiang City. 20L plastic pots were used, each containing 15kg of dry soil and 5 rice grains. The treatment steps were as follows: Initially, distilled water was used to irrigate to a depth of 3cm above the soil surface, and then water was replenished every 5 days to maintain a depth of 3cm. The indoor air temperature was maintained at 22±2℃ during the day and 18±2℃ at night; the photoperiod was 12h, and the light intensity was 950umol / m²·s; the relative humidity was 45±5% during the day and 70±5% at night. Before planting the rice, the soil was treated with three different application rates and one treatment with only chemical fertilizer. Each treatment was tested in triplicate. The germination rate and growth of the rice plants were monitored over 8 weeks, and the physicochemical properties of the soil in each treatment group were also measured.

[0087] The statistical results of the physiological indicators of rice plants are shown in the table below: (Medium and high doses show the best effect)

[0088]

[0089] Application Example 2

[0090] The effect of the soil conditioner prepared in Example 1 on improving albic soil in dryland fields was determined by a greenhouse pot experiment:

[0091] The test soil was alkaline soil from dry fields in Tongjiang City. 20L plastic pots were used, each containing 15kg of dry soil and planted with 3 corn kernels. The treatment procedure was as follows: irrigation with distilled water to 60% moisture content began at the start of the experiment, followed by 50mL of distilled water every 7 days thereafter; the air temperature in the growing room was maintained at 22±2℃ during the day and 18±2℃ at night; the photoperiod was 12h, and the light intensity was 950umol / m²·s; the relative humidity was 45±5% during the day and 70±5% at night. Three application doses were applied to the soil for rice cultivation, with each treatment performed in triplicate. The germination rate and growth of rice plants were measured over 8 weeks, and the physicochemical properties of the soil in each treatment group were also tested.

[0092] The statistical results of the physiological indicators of maize plants are shown in the table below: (Higher doses produce the best results)

[0093]

[0094] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0095] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A soil conditioner based on a microbial-animal interaction network, characterized in that, It includes the following components: nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, auxiliary microorganisms, soil animals, carrier substances, nutrients, and regulators, wherein, by weight fraction: The nitrogen-fixing microorganisms: 1-3 portions; Phosphate-solubilizing microorganisms: 1-3 parts; Potassium-solubilizing microorganisms: 1-3 parts; The auxiliary microorganisms: 1-3 parts; The soil animals: 6-12 parts; The carrier substance: 10-20 parts; The stated nutrients: 3-5 servings; The regulator: 2-4 parts.

2. The alkaline soil conditioner based on a microbial-animal interaction network according to claim 1, characterized in that: The nitrogen-fixing microorganism is selected from *Azospirillum*, the phosphate-solubilizing microorganism is selected from *Pseudomonas*, which is a high-yield species of extracellular polysaccharides, the potassium-solubilizing microorganism is selected from silicate bacteria, the auxiliary microorganisms include putrefactive microorganisms, growth-promoting microorganisms, disease-resistant microorganisms and arbuscular mycorrhizal fungi, the putrefactive microorganisms are selected from *Bacillus amyloliquefaciens* and *Laurella vegetans*, the growth-promoting microorganisms are selected from *Bacillus belye* and *Bacillus subtilis*, and the disease-resistant microorganism is selected from *Streptomyces*.

3. The alkaline soil conditioner based on a microbial-animal interaction network according to claim 1, characterized in that: The soil animals are selected from one or more of earthworms, nematodes, and ants.

4. The alkaline soil conditioner based on a microbial-animal interaction network according to claim 1, characterized in that: The carrier material is a mixture of straw and earthworm castings, which are mixed in a 2:2 ratio to form the carrier material.

5. The alkaline soil conditioner based on a microbial-animal interaction network according to claim 1, characterized in that: The nutrients include urea, superphosphate, and potassium sulfate, which are used as inorganic fertilizers in a ratio of 21:15:

16.

6. The alkaline soil conditioner based on a microbial-animal interaction network according to claim 1, characterized in that: The regulator is selected from humic acid and biochar, and is applied according to the soil acidity or alkalinity. For acidic soil, it is used at a ratio of 1:2; for neutral soil, it is used at a ratio of 1:1; and for alkaline soil, it is used at a ratio of 2:

1.

7. The alkaline soil conditioner based on a microbial-animal interaction network according to claim 1, characterized in that: The soil conditioner is applied evenly to the soil of corn, wheat, and vegetable gardens at a dosage of 100-150 kg per hectare.

8. A method for preparing a limestone amendment based on a microbial-animal interaction network, characterized in that, The main steps include: Step 1: Microbial sampling Use a shovel to collect good quality soil, then mix and dissolve the collected soil in pure water to prepare a suspension, which allows microorganisms to be released and dispersed. Then filter the prepared suspension to remove impurities, and divide the processed suspension into four equal parts and put them into four different petri dishes. Step 2: Enrichment Culture Neutral enrichment nutrient solution and inhibitors that inhibit the growth of other microorganisms were added to four petri dishes respectively. The petri dishes were then placed at room temperature for one week to allow the microorganisms in the four petri dishes to multiply in large quantities. Step 3: Purebred Isolation The microbial suspensions in the four petri dishes were serially diluted and then inoculated into four different solid culture media. Then, the nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms and helper microorganisms in the solid culture media were isolated by dilution plating method, so that the individual microorganisms formed independent colonies on the culture media. Step 4: Filtering The growth rate, metabolites, and enzyme activities of different independent colonies were tested. Then, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and helper microorganisms with consistent morphology, clear edges, smooth surfaces, and good colony growth were screened and placed in slant culture medium and stored at room temperature. Step 5: Collection of Soil Animals Collect an appropriate amount of earthworms, then clean the collected earthworms to remove impurities and pathogens. Then select earthworms that are healthy, active, and similar in size and shape and put them into a culture tank and store them at room temperature. Step Six: Raw Material Preparation Select pure straw and earthworm castings, then mix them evenly in a 2:2 ratio. Next, add three inorganic fertilizers, namely urea, superphosphate and potassium sulfate, in a 21:15:16 ratio. At the same time, add two organic fertilizers, namely humic acid and amino acids, in a 1:1 ratio. Finally, mix them thoroughly to obtain the mixture. Step 7: Soil amendment preparation The pH value of the mixture was tested, and then fulvic acid and biochar were added according to the measured pH value. The ratio was 1:2 for acidic soil, 1:1 for neutral soil, and 2:1 for alkaline soil. By using fulvic acid and biochar as regulators, the pH value of the mixture was adjusted to maintain a neutral pH. Subsequently, soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms were added to the mixture in sequence. The mixture was then placed in a tank for cultivation at room temperature. At the same time, a multi-layer biological network analysis method was used to construct a soil fertility network for albic soil. Key microbial nodes were identified to regulate the soil microbial community, allowing soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms to reproduce fully. This ensured that soil animals, nitrogen-fixing microorganisms, phosphorus-solubilizing microorganisms, potassium-solubilizing microorganisms, and auxiliary microorganisms coexisted harmoniously, forming a stable microbial-animal symbiotic system. In this way, an albic soil conditioner based on a microbial-animal interaction network can be prepared. Step 8: Packaging The prepared soil conditioner is packaged in equal amounts into bags of the same size for easy use.

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