A hundred nanometer level particle sorting manipulation chip, device and sorting manipulation method

Abstract

The present application relates to a kind of hundred nanometer level particle sorting manipulation chip, device and sorting manipulation method, the chip includes microlens, microfluidic channel, sorting outlet and sorting inlet, the sorting outlet is bifurcation, the sorting inlet is trifurcation, including sample flow inlet and 2 focus flow inlets;Sorting inlet and sorting outlet are respectively arranged at the two ends of microfluidic channel, and microlens is arranged at the intersection of 2 sorting outlets.The device includes chip, light source, injection device and observation platform, and the light emitted by light source is irradiated into microfluidic channel through microlens, and light force is applied to the particle in the fluid to be sorted, and different size particles enter different sorting outlets, finally realize sorting.Compared with prior art, the present application can realize the rapid sorting of hundred nanometer level particle, with the advantages of high sorting efficiency, high control precision, etc., and has important application value for the sorting and manipulation of micro-nano scale particles such as virus and bacteria.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of micro-nanoparticle manipulation and biological detection, and particularly relates to a hundred-nanometer particle sorting and manipulation chip, device and sorting and manipulation method. BACKGROUND

[0002] High-precision manipulation and sorting of micro-nanoparticles have important application value in the fields of biomedical detection, drug delivery, environmental monitoring, etc., especially in the range of hundreds of nanometers (100-400 nm), such as sorting of viruses (typical size 50-300 nm), exosomes (30-150 nm) and nanomedicine carriers (100-400 nm), which directly affects the accuracy of subsequent detection and application.

[0003] Traditional particle manipulation technologies mainly include electrophoresis, magnetophoresis, etc. Traditional sorting methods, such as filtering, flow cytometry and size elution, more or less have the disadvantages of poor sorting precision, low sorting efficiency, and difficulty in sorting nanoscale particles. Electrophoresis relies on the charge difference of particles, and has low resolution and poor sorting precision for electrically neutral or low-charge hundred-nanometer particles; magnetophoresis requires modification of the magnetism of particles, which destroys the biological activity; the filtering method is easy to block and cannot realize continuous sorting, and has low sorting efficiency.

[0004] Optical sorting technology is expected to break through these limitations and stand out among a series of sorting methods due to its advantages of high resolution, non-invasiveness and nanoscale precision, making high-precision and high-accuracy particle manipulation and sorting possible. In recent years, optical flow control technology combines the advantages of microfluidics and optical manipulation, providing a new idea for high-throughput and high-precision sorting of micro-nanoparticles. However, existing optical flow control methods still face challenges in the sorting of hundred-nanometer scale particles. Traditional optical tweezers technology can only achieve single-point manipulation, and has low throughput; mainstream optical flow control technology relies on polarization-sensitive structures (such as gratings, superstructures), and changes in incident light polarization state will cause fluctuations in sorting efficiency, and the sorting precision for 100-400 nm particles is generally poor, which cannot meet the needs of biological detection. Most methods are only suitable for particles of specific materials (such as gold nanoparticles), and cannot be compatible with common carrier particles such as polystyrene, silica, and biological particles such as viruses and bacteria, and have poor versatility.

[0005] At present, there is still a lack of a micro-nanoparticle sorting method with high precision and high throughput, which limits its wide application in the fields of biological detection and nanomedicine. SUMMARY

[0006] The present application aims to overcome the defects of the prior art, and provides a hundred nanometer particle sorting and manipulation chip, device and sorting and manipulation method. The present application relates to the technical field of micro-nano particle manipulation and biological detection, and particularly relates to a high-precision sorting method for hundred nanometer scale (100-400 nm) polystyrene particles based on optical flow control technology, which can be applied to efficient sorting and manipulation of biological micro-nano particles such as viruses, bacteria and exosomes, and is suitable for biomedical detection, nano-drug screening, environmental micro-pollutant analysis and the like.

[0007] The present application aims to solve the three core problems in the prior art: unable to balance high-precision sorting and high-throughput continuous operation of hundred nanometer particles; the existing optical flow control system relies on polarization-sensitive structures, has poor stability, is significantly affected by the polarization state of the light source; and the application range is narrow, and it is difficult to be expanded to the sorting of biological micro-nano particles (such as viruses and bacteria), and the repeatability error is large.

[0008] The object of the present application can be achieved by the following technical solutions:

[0009] One of the technical solutions of the present application provides a hundred nanometer particle sorting and manipulation chip based on optical flow control technology, wherein the chip is a microfluidic chip, comprising a microlens, a microfluidic channel, a sorting outlet and a sorting inlet, the sorting outlet is a two-way outlet, comprising a first sorting outlet and a second sorting outlet, the sorting inlet is a three-way outlet, comprising a sample flow inlet, a first focusing flow inlet and a second focusing flow inlet; the sorting inlet and the sorting outlet are respectively arranged at two ends of the microfluidic channel, for the entry and exit of the fluid to be sorted; the microlens is arranged at the intersection of the first sorting outlet and the second sorting outlet, and the microlens is used to convert the transmitted Gaussian light into linear light and irradiate into the microfluidic channel to exert optical force on the particles in the fluid to be sorted in the microfluidic channel; after the fluid to be sorted is sorted through the microfluidic channel, particles of different sizes will enter the first sorting outlet or the second sorting outlet respectively.

[0010] The second technical solution of the present application provides a hundred nanometer particle sorting and manipulation device based on optical flow control technology, comprising the above sorting and manipulation chip, a light source, a fluid to be sorted injection device, a focusing fluid injection device and an observation platform for observing the motion state of particles; the observation platform comprises a microscope and a camera, which are used to observe the particle motion trajectory and sorting result in real time.

[0011] Further, the light source is used to emit Gaussian light.

[0012] Further, the microlens is used to shape the Gaussian light beam emitted by the light source into a highly bound linear light field, and the microlens is a polygonal prism.

[0013] Further, the microlens is an isosceles triangular prism, and the microlens is a PDMS cylindrical lens; the isosceles triangular prism has a size of: a length of a base of the triangle is 27-65 μm (preferably 45 μm), a height of the base of the triangle is 30-50 μm (preferably 45 μm), and a height of the microlens is 50-80 μm.

[0014] Further, in some other embodiments of the present application, the microlens is an axisymmetric polygonal prism, which can be used to generate a linear light field.

[0015] Further, the microfluidic channel is a PDMS channel layer with a thickness of 50-80 μm; an inner wall of the microfluidic channel is treated to be hydrophilic.

[0016] Further, the sample inlet is connected to a to-be-sorted fluid injection device, the first focusing inlet and the second focusing inlet are connected to a focusing fluid injection device; the to-be-sorted fluid injection device and the focusing fluid injection device are respectively controlled by an external pump, for focusing and adjusting the fluid flow rate.

[0017] In some specific embodiments, the to-be-sorted fluid injection device and the focusing fluid injection device only need to meet the function of fluid injection, and are generally composed of a pipeline and a pump, and the pump can be a syringe pump, a peristaltic pump, a laminar pump, etc.

[0018] The third technical solution of the present application provides a preparation method of a hundred-nanometer-level particle sorting and manipulation chip based on optical fluid control technology, the chip is composed of a PDMS layer and a substrate layer, and the preparation steps are as follows: first, a mold is designed and manufactured by a 3D printer, and a PDMS layer with a channel is obtained by a mold turning method, the channel includes the microfluidic channel, the sorting outlet, and the sorting inlet, and the PDMS layer integrally integrates the microlens; then, the side with the channel is adhered to the substrate layer to obtain the sorting and manipulation chip; the substrate layer is made of a high-transmittance material, which is convenient for observation.

[0019] The fourth technical solution of the present application provides a hundred-nanometer-level particle sorting and manipulation method based on optical fluid control technology, based on the above sorting and manipulation chip and device, and the method includes the following steps:

[0020] S1. Preparing materials: selecting to-be-sorted materials, pretreating the to-be-sorted materials to form to-be-sorted fluid, and ensuring that the particles in the to-be-sorted fluid are uniformly dispersed and have no agglomeration phenomenon, for standby use;

[0021] S2. Beam shaping: shaping a Gaussian beam emitted by a light source into a linear light field by a microlens, and entering the microfluidic channel, and adjusting the power of the light source;

[0022] S3. Sorting: injecting the prepared fluid to be sorted into the sample inlet, focusing the fluid into the first focusing inlet and the second focusing inlet, and adjusting the flow rates of the fluid to be sorted and the focusing fluid;

[0023] S4. Collection: when sorting is performed by using the sorting manipulation chip, target particles are separated according to their sizes and are guided to the first sorting outlet and the second sorting outlet, respectively, and then, the sample of the required size is collected at the corresponding outlet, thereby achieving efficient sorting.

[0024] The fifth technical solution of the present application provides an application of the sorting manipulation chip, the sorting manipulation device and the sorting manipulation method, which is used for screening of particles in the order of hundreds of nanometers. The particles in the order of hundreds of nanometers can be biological particles such as viruses, bacteria, cells and exosomes, and also include non-biological particles such as gold nanoparticles and silica particles, and are not limited to these.

[0025] Compared with the prior art, the present application has the following beneficial effects:

[0026] 1. The chip integrated light field manipulation technology provided by the present application realizes efficient sorting of polystyrene particles in the range of 100-400 nm by optimizing the microfluid channel structure and light field distribution. Compared with the single-point manipulation of the traditional optical tweezers, the present application utilizes the synergistic effect of the linear light potential well array and the fluid shear force to realize continuous and high-throughput particle sorting in the microfluid channel.

[0027] 2. The present application uses non-polarization-dependent and light field and microfluid channel geometry optimization, so that the light field distribution is not sensitive to the polarization of incident light, is suitable for natural light or non-polarized light source, and significantly improves the stability and applicability of the system, avoiding the polarization-sensitive structure (such as grating, super surface) commonly used in the existing optical flow control sorting technology, which affects the sorting efficiency.

[0028] 3. The present application is not only suitable for the polystyrene particles provided in the embodiments, but also can be extended to the manipulation and sorting of various nanoscale targets such as gold nanoparticles, silica particles, exosomes, viruses and the like by adjusting the light field parameters (such as wavelength, intensity) and the flow channel design, and has wide application prospects in the fields of biomedical detection, nanodrug screening and the like. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 The structure diagram of the particle sorting manipulation chip in the order of hundreds of nanometers based on the optical flow control technology of the present application embodiment (a) and the particle force principle diagram (b); Figure 1 Figure 1

[0030] Figure 2 The method flow chart for optimizing the chip in the present application embodiment; ​​

[0031] Figure 3 This is a sorting trajectory diagram of 200nm and 400nm polystyrene particles under the combined action of fluid force, scattering force and gradient force in an embodiment of the present invention;

[0032] Figure 4 A diagram showing the fabrication results of the optofluidic chip according to an embodiment of the present invention. Figure 4 (a) and the focused beam pattern constructed in the experiment ( Figure 4 (b)

[0033] Figure 5 This is a schematic diagram illustrating the structure of a linear light field constructed using a scanning lens according to an embodiment of the present invention, wherein... Figure 5 Image 'a' is a lens model diagram built using FDTD software. Figure 5 In section b, the focused line light field pattern was calculated through simulation using FDTD by optimizing the lens structure. Figure 5 In the diagram, c represents the dimensions of the hexagonal prism used for simulation.

[0034] Figure 6 This is a comparison diagram of the forces experienced by polystyrene particles of different sizes at the same optical field position, as shown in this embodiment of the invention. It illustrates the scattering forces experienced by polystyrene particles with diameters of 200 nm and 400 nm under the same optical field conditions. Figure 6 a) and gradient force ( Figure 6 Diagram showing the size difference in (b) of the image;

[0035] Figure 7 This is a sorting result diagram of an embodiment of the present invention, showing green polystyrene particles with a diameter of 200 nm. Figure 7 (a) and red polystyrene particles with a diameter of 400 nm ( Figure 7 (b) Sorting effect diagram of the cells being sorted into different channels. Detailed Implementation

[0036] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. These embodiments are based on the technical solution of the present invention and provide detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following embodiments.

[0037] Example 1: A 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology

[0038] Addressing the particle sorting size and speed issues mentioned in the background section, achieving the smallest possible sorting size while maintaining high sorting speed is a bottleneck problem that urgently needs to be solved for the further development of micro / nano particle manipulation and biosensing technology. Therefore, it is necessary for researchers in this field to develop a high-precision optofluidic sorting platform, thereby realizing an optofluidic chip platform with a clear physical mechanism, simple construction concept, and excellent sorting effect.

[0039] This invention first provides a 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology. The device includes a sorting and manipulation chip (including a microlens 2, a microfluidic channel 3, a sorting outlet, and a sorting inlet), a light source 1, a fluid injection device for sorting, a focusing fluid injection device, and an observation platform for observing the particle motion state. Specifically, it includes:

[0040] like Figure 1 As shown in Figure a, the system includes a light source 1, a microlens 2, a microfluidic channel 3, a sorting outlet, and a sorting inlet. The sorting outlet includes a first sorting outlet 41 and a second sorting outlet 42, i.e., a two-way outlet. The sorting inlet includes a sample flow inlet 51, a first focusing flow inlet 52, and a second focusing flow inlet 53, i.e., a three-way inlet. The sorting inlet and outlet are respectively located at both ends of the microfluidic channel 3 for the entry and exit of the fluid to be sorted. The microlens 2 is located at the intersection of the first sorting outlet 41 and the second sorting outlet 42. The light source 1 emits Gaussian light, which passes through the microlens 2 and irradiates the microfluidic channel 3, applying photodynamic force to the particles in the fluid to be sorted within the microfluidic channel 3.

[0041] The light source 1 consists of a laser, an optical fiber coupler, and an optical fiber. The laser emits Gaussian light, and the incident direction of the light source is tilted in the plane of the chip, i.e., not parallel to the axis of the microfluidic channel 3. The microlens 2 is used to shape the Gaussian beam emitted from the light source 1 into a highly confined linear light field. The microlens 2 is an isosceles triangular PDMS cylindrical lens with dimensions of 27-65 μm for the length of the base, 30-50 μm for the height of the base, and 50 μm for the height of the microlens 2 (preferably, both the length of the base and the height of the base are 45 μm in subsequent embodiments). The microfluidic channel 3 is a PDMS channel layer with a thickness of 50 μm, which is used for fluid passage. The inner wall of the microfluidic channel 3 is hydrophilically treated. The sample inlet 51 is connected to the fluid injection device to be sorted, and the first focusing inlet 52 and the second focusing inlet 53 are connected to the focusing fluid injection device. The fluid injection device to be sorted and the focusing fluid injection device are controlled by three external injection pumps for focusing and adjusting the fluid flow rate. After the fluid to be sorted is sorted through the microfluidic channel 3, particles of different sizes will enter the first sorting outlet 41 or the second sorting outlet 42 respectively, and finally achieve sorting.

[0042] The chip is composed of a PDMS layer and a substrate layer: first, a mold is designed and made, and a PDMS layer with channels is obtained by the reverse molding method, the channels including the microfluidic channel 3, the sorting outlet, and the sorting inlet, and the PDMS layer integrally integrating the microlens 2; specifically, a resin mold is used, PDMS prepolymer is mixed with a curing agent at a mass ratio of 10:1, and after curing at 80°C for 2 hours, demolding is performed. Then, the side with the channels is adhered to the substrate layer, which is made of glass or other high-transmittance materials, for easy observation.

[0043] The device is also provided with an observation platform for observing the motion state of the particles, which includes an inverted fluorescence microscope (parameter settings: magnification 40x, numerical aperture 1.4) and a high-speed camera (parameter settings: frame rate 100 fps), for real-time observation of the particle motion trajectory and sorting results.

[0044] As shown in Figure 1 b, the particle in the fluid to be sorted enters the microfluidic channel 3 and is subjected to force analysis near the microlens 2. The particle is subjected to three key forces: fluid force (F drag ), scattering force (F sca ), and gradient force (F gra ). Particles of different diameters are subjected to different sizes of the three key forces in the microfluidic channel 3, and are thus sorted into different outlets.

[0045] Example 2: A method for sorting and manipulating particles at the order of 100 nanometers based on optical fluidic technology

[0046] Based on the device in Example 1, a method for sorting and manipulating particles at the order of 100 nanometers based on optical fluidic technology is provided, which generates a focused light field by designing the size of the microlens and interacts with the particles, thereby realizing high-precision particle sorting under the cooperation of the fluid.

[0047] The steps of the method are shown in Figure 2 , including:

[0048] 1) Construct a linear light field micro-nano manipulation system: optimize the light source parameters and the shape and position of the microlens 2 to generate a highly bound linear light field in the microfluidic channel 3 to realize precise manipulation of the particles;

[0049] 2) Analyze the force and motion characteristics of the particles: based on the differences in the optical forces of particles of different sizes in the linear light field, establish a particle motion model, and determine the optimal microlens design and light field conditions through light field simulation, and analyze the force and characteristics of particles of different sizes;

[0050] 3) Achieving high-precision sorting of nanometer-scale particles: using the differential effect of linear light field on different microparticles, forming a sorted microparticle flow, realizing rapid separation of particles, and achieving efficient sorting and manipulation of micro-nanoparticles.

[0051] The mode particles used in this embodiment are polystyrene particles, and the size range of the polystyrene particles used is 100-400 nm, with a precision of 200 nm. In this embodiment, the ideal light field is a thin and regular linear light field. After determining the optimal microlens design and light field conditions through light field simulation, based on the differences in light force received by particles of different sizes in the linear light field, a particle force analysis and calculation is established to analyze the force size and characteristics of 100-400 nm scale polystyrene particles. First, the gradient force and scattering force received by the particles in the linear light field are calculated (by substituting the light field intensity distribution, particle size, etc.), and then fluid dynamics parameters (such as the viscosity of the fluid used in the experiment, the flow field velocity) are introduced to couple the gradient force, scattering force and flow field force, and a complete particle motion model is constructed. Through model calculation, the force size, direction and size variation characteristics of different size (100 nm, 200 nm, 300 nm, 400 nm, etc.) polystyrene particles are analyzed to form theoretical analysis data. The binding strength of the linear light field can be optimized by adjusting the laser power and microlens focal length, and the particle motion model is based on gradient force and scattering force calculation combined with fluid dynamics simulation.

[0052] The sorting and manipulation method of this embodiment is as follows: the light generated by the light source 1 will produce a highly bound linear light field in the microfluidic channel 3 after passing through the microlens 2 integrated on the chip. Different sizes of microparticles will receive very different scattering forces (such as Figure 1 F sca in b of FIG. 1) and gradient forces (such as Figure 1 F gra in b of FIG. 1), and the fluid force (such as Figure 1 F drag in b of FIG. 1) is not much different, so different particles receive different dominant forces, large particles are dominated by gradient force, and small particles are dominated by fluid force, ultimately leading to a large difference in their motion trajectories in the microfluidic channel 3 (flow field).

[0053] Specifically, based on the above-optimized chip, the embodiment provides a practical sorting and manipulation method, which includes the following steps:

[0054] S1. Preparation of materials: select 100-400 nm scale polystyrene particles as the material to be sorted (as the mode particles of this embodiment, other particles can be used in other embodiments), pretreat the particles (such as ethanol cleaning to remove surface impurities, ultrasonic dispersion treatment), ensure uniform dispersion and no agglomeration, and reserve for use;

[0055] S2. Sorting of particles in the chip:

[0056] According to the size range of the fluid to be sorted (in this embodiment, 100-400 nm scale), the chip is designed and placed in the observation platform, the light source 1 is turned on, a highly constrained linear light field is generated in the microfluidic channel 3, the fluid inlet and the fluid outlet are connected, and the fluid to be sorted and the focusing fluid are introduced; at this time, different size particles enter the first sorting outlet 41 and the second sorting outlet 42 respectively due to different forces.

[0057] The sorting accuracy in step S2 can be further optimized by adjusting the size of the lens to optimize the light field gradient, adjusting the sample flow, and adjusting the flow rate and particle concentration of the focusing flow, to achieve smaller sorting accuracy and sorting diameter, and can be applied to the sorting of virus particles, bacteria and other biological particles.

[0058] Experimental results:

[0059] As shown in Figure 3 , the sample particles are focused by the fluid on both sides, and then the different effects of the linear light field on different particles form a sorted particle flow. The 200 nm diameter polystyrene particles are sorted into the first sorting outlet 41 due to the dominant effect of fluid force; while the 400 nm diameter polystyrene particles are sorted into the second sorting outlet 42 under the dominant effect of light force, thereby realizing the rapid separation of polystyrene particles with 200 nm accuracy, and being suitable for efficient sorting and manipulation of virus, bacteria and other micro-nano particles. The results show that by designing the shape of the microfluidic channel and the flow rate of the three inlets, different particle sizes will flow to different outlets under the combined action of fluid force and light force, and the sorting efficiency is 100%.

[0060] This embodiment further verifies the sorting chip and method described above:

[0061] Figure 4 The schematic diagram of the optofluidic chip manufactured for the experiment is shown in Figure 4 a), and the linear light beam diagram constructed after the Gaussian light is focused by the lens in the experiment is shown in Figure 4 b).

[0062] Figure 5Figure a shows a schematic diagram of the structure of the linear light field constructed by the scanning lens size. The linear light field is constructed using Ansys Lumerical FDTD (hereinafter referred to as FDTD). Figure 5 Figure b illustrates the constructed linear light field. (e.g., ...) Figure 5 As shown in Figure c, the microlens 2 used for simulation in the software is an axisymmetric hexagonal prism. The hexagonal prism has four dimensions: x, y, z, and h. The values ​​of x are 2, 3, 4, and 5 µm; the values ​​of y are 15, 20, and 25 µm; the values ​​of z are 15, 20, and 25 µm; and the values ​​of h are 10, 15, 20, 25, 30, and 35 µm.

[0063] Figure 6 The image shows a comparison of the forces acting on particles of different sizes (400 nm and 200 nm in diameter) at the same optical field position, calculated using FDTD software. The horizontal axis in the image represents the distance from the particle to the rightmost side of microlens 2 (here, "rightmost side" refers to...). Figure 1 Using the right vertex of the triangle in the diagram as a reference, it can be observed that the magnitude of the light force received by particles varies with their size. Figure 6 The scattering force shown in Figure a indicates that for polystyrene particles with diameters of 400 nm and 200 nm, the scattering force gradually decreases with increasing distance from the particle to the rightmost side of the microlens. At the same distance, the scattering force of the 400 nm particle is much greater than that of the 200 nm particle. For example, when X = 60 µm, the scattering force of the 400 nm particle is approximately 1.5 pN, while that of the 200 nm particle is only about 0.2 pN. Figure 6 The gradient force shown in Figure b indicates that for 200nm particles, the gradient force increases slowly from approximately -0.2 pN with increasing distance X and then stabilizes with a small change. For 400nm particles, the gradient force increases rapidly from approximately -1.4 pN with increasing distance X (the absolute value decreases), eventually approaching -0.2 pN. At the same distance, the gradient force (absolute value) of 400nm particles is much greater than that of 200nm particles, and the change with distance is more significant. This shows that the closer the particles are to the microlens, the greater the downward force they experience, exceeding the fluid force, thus allowing them to be sorted into the second sorting outlet 42.

[0064] Figure 7 This is a diagram showing the sorting results of an embodiment of the present invention. Figure 7 The green particles shown in Figure a are polystyrene particles with a diameter of 200 nm. Figure 7 The red particles shown in b are polystyrene particles with a diameter of 400 nm. Experimental observations show that the green particles mainly migrate towards the first sorting outlet 41, while the red particles tend to be transported towards the second sorting outlet 42 due to the effect of the light field.

[0065] The structure parameters of the optical flow control chip in the application are not limited to this, and the lens size / micro-channel width / height and design can be reasonably adjusted according to the particle size according to different particle sorting sizes and light fields.

[0066] Example 3 realizes high-precision hundred-nanometer polystyrene particle sorting

[0067] In this embodiment, the sorting operation chip and the sorting operation method provided in the above embodiments are applied to the sorting of actual materials (such as biological materials such as viruses, bacteria, and materials of appropriate sizes not mentioned in the application). The specific steps are as follows:

[0068] S1. Prepare materials: select the material to be sorted, pretreat the material to be sorted (such as selecting a suitable solvent to clean according to the particle characteristics to remove surface impurities, treating by ultrasonic or other dispersion means), and prepare the fluid to be sorted, ensure that the particles in the fluid to be sorted are uniformly dispersed and have no agglomeration phenomenon, and reserve;

[0069] S2. Light beam shaping:

[0070] The Gaussian light beam emitted by the light source 1 is shaped into a linear light field by the microlens 2, enters the microfluidic channel 3, and adjusts the power of the light source 1 to 380 mW;

[0071] S3. Sorting:

[0072] The fluid to be sorted prepared in step S1 is injected into the sample flow inlet 51 (through the injection pump), ultrapure water is injected into the first focusing flow inlet 52 and the second focusing flow inlet 53, and the flow rate of the sample flow inlet 51 is adjusted to 0.81 μm / s, and the flow rates of the two focusing flow inlets are 32.4 μm / s and 26 μm / s, respectively;

[0073] S4. Collection:

[0074] When sorting is performed using the sorting operation chip, the target particles will be separated according to their sizes and directed to the first sorting outlet 41 and the second sorting outlet 42, respectively, and then the sample of the required size is collected at the corresponding outlet, thereby realizing efficient sorting.

[0075] The sorting efficiency in this embodiment reaches 25 particles per minute.

[0076] The above detailed the preferred embodiments of the application. It should be understood that those skilled in the art can make many modifications and changes without creative labor according to the concept of the application. Therefore, any technical solution obtained by logical analysis, reasoning, or limited experiment by those skilled in the art on the basis of the prior art according to the concept of the application should be within the scope of protection determined by the claims.

Claims

1. A 100-nanometer-scale particle sorting and manipulation chip based on optofluidic technology, characterized in that, The chip is a microfluidic chip, including: a microlens (2), a microfluidic channel (3), a sorting outlet and a sorting inlet. The sorting outlet is a two-way outlet, including a first sorting outlet (41) and a second sorting outlet (42). The sorting inlet is a three-way outlet, including a sample flow inlet (51), a first focusing flow inlet (52) and a second focusing flow inlet (53). The sorting inlet and sorting outlet are respectively set at both ends of the microfluidic channel (3) for the entry and exit of the fluid to be sorted; the microlens (2) is set at the intersection of the first sorting outlet (41) and the second sorting outlet (42), and the microlens (2) is used to convert the transmitted Gaussian light into linear light and irradiate it into the microfluidic channel (3) to apply photodynamic force to the particles in the fluid to be sorted in the microfluidic channel (3); After the fluid to be sorted is sorted through the microfluidic channel (3), particles of different sizes will enter the first sorting outlet (41) or the second sorting outlet (42) respectively. By utilizing the synergistic effect of linear optical potential trap arrays and fluid shear force, continuous, high-throughput particle sorting is achieved within microchannels.

2. A device for sorting and manipulating 100-nanometer-scale particles based on optofluidic technology, characterized in that, The device includes: the sorting control chip as described in claim 1, a light source (1), a fluid injection device for sorting, a focusing fluid injection device, and an observation platform for observing the motion state of particles; the observation platform includes a microscope and a camera for real-time observation of particle motion trajectory and sorting results.

3. The 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology according to claim 2, characterized in that, The light source (1) is used to emit Gaussian light.

4. The 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology according to claim 3, characterized in that, The microlens (2) is used to shape the Gaussian beam emitted from the light source (1) into a highly confined linear light field. The microlens (2) is a polygonal prism.

5. The 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology according to claim 4, characterized in that, The microlens (2) is an isosceles triangular prism and a PDMS cylindrical lens. The dimensions of the isosceles triangular prism are: the length of the base of the triangle is 27-65μm, the height of the base of the triangle is 30-50μm, and the height of the microlens (2) is 50-80μm.

6. The 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology according to claim 2, characterized in that, The microfluidic channel (3) is a PDMS channel layer with a thickness of 50-80 μm; the inner wall of the microfluidic channel (3) is hydrophilically treated.

7. The 100-nanometer-scale particle sorting and manipulation device based on optofluidic technology according to claim 2, characterized in that, The sample inlet (51) is connected to the fluid injection device to be sorted, and the first focusing inlet (52) and the second focusing inlet (53) are connected to the focusing fluid injection device. The fluid injection device to be sorted and the focusing fluid injection device are controlled by external pumps to focus and adjust the fluid flow rate.

8. A method for sorting and manipulating 100-nanometer-scale particles based on optofluidic technology, characterized in that, Based on the sorting control device according to any one of claims 2-7, the method includes the following steps: S1. Material preparation: Select the material to be sorted, pre-treat the material to be sorted and prepare it into a fluid to be sorted, ensuring that the particles in the fluid to be sorted are evenly dispersed and there is no agglomeration, and set it aside for later use; S2. Beam shaping: The Gaussian beam emitted from the light source (1) is shaped into a linear light field through a microlens (2), enters the microfluidic channel (3), and the power of the light source (1) is adjusted. S3. Sorting: Inject the fluid to be sorted obtained in step S1 into the sample inlet (51), inject the focusing fluid into the first focusing inlet (52) and the second focusing inlet (53), and adjust the flow rates of the fluid to be sorted and the focusing fluid. S4. Collection: When sorting is performed using the sorting control chip, the target particles are separated according to their size and guided to the first sorting outlet (41) and the second sorting outlet (42) respectively. Then, the samples of the required size are collected at the corresponding outlets, thereby achieving efficient sorting.

9. The application of the 100-nanometer-scale particle sorting and control chip based on optofluidic technology as described in claim 1, or the sorting and control device as described in any one of claims 2-7, or the sorting and control method as described in claim 8, characterized in that, Used for screening particles at the 100-nanometer scale, which are selected from biological or non-biological particles.

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