Ceramide and plant extract composite synergistic repair system based on bionic liposome and application of ceramide and plant extract composite synergistic repair system

Abstract

The invention relates to a ceramide and plant extract composite synergistic repair system based on bionic lipidosome and application thereof. The ceramide and plant extract composite synergistic repair system comprises ceramide bionic lipidosome, saccharomycetes rice fermentation filtrate, witch hazel extract and saccharide isomeride according to the mass ratio of (0.1-3): (0.1-5): (0.01-0.5): (0.1-5). The composition is used in skin care products. Compared with the prior art, a multi-element repairing system of bionic structure matching, inflammation resisting, moisture and temperature adjusting and cell metabolism activating is formed, the skin state can be improved from multiple dimensions, and remarkable advantages are achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of cosmetics and personal care, and specifically relates to a repair composition taking ceramide biomimetic liposome and plant complex as core components and application thereof. In the field of cosmetics and personal care, the composition can be used for repair of sensitive skin, wound healing and repair of damaged skin barrier. BACKGROUND

[0002] With the rapid development of society and the gradual advancement of industrialization, people's living standards have improved, and the quality of life is getting better and better, and the lifestyle is becoming rich and varied. At the same time, industrialization and human activities have also brought about environmental pollution, ultraviolet radiation, light pollution and other problems, which have had an undeniable impact on the skin condition. According to relevant data, about 30%-40% of the global population has varying degrees of skin problems, which are manifested as dry skin, redness, itching and other symptoms. Skin is the body's natural barrier and is a very important part of physical health. Seeking ways to improve skin condition is slowly becoming one of the focuses of attention.

[0003] In recent years, the medical and beauty industry has developed rapidly, providing consumers with a variety of cosmetics and skin care products, and their functions have also changed from single whitening effect to moisturizing, repairing and other comprehensive effects. Market research data shows that the global cosmetics industry market size in 2024 will exceed 18 billion US dollars, and is growing at a rate of about 8% per year. At the same time, in the face of a wide range of cosmetics and skin care products, consumers have also begun to demand higher safety and scientificity of their ingredients. When purchasing products, consumers tend to choose natural or clinically proven products, which has to some extent also promoted the progress of the cosmetics industry.

[0004] However, traditional repair products mostly rely on single active ingredients, without considering the multi-dimensional needs of skin barrier repair, such as lipid supplementation, anti-inflammatory synergy and other processes, making their effect on skin repair limited. In addition, some repair products have multiple functional ingredients, but due to the lack of effective penetration medium, the functional ingredients are mostly difficult to penetrate the stratum corneum and only stay on the surface of the skin, making it difficult to act deeply.

[0005] As disclosed in patent CN113662879B, a salt-tolerant ceramide liposome with skin repair effect comprises the following raw material components by mass percentage: 2-20% of ceramide mixture, 0.01-1% of salicyl sphingosine, 0.01-2% of phytosphingosine, 0.5-5% of cholesterol, 0.01-20% of vegetable oil, 8-30% of hexyldecanol, 5-60% of water, 10-70% of polyol, and 2-10% of liposome membrane material, wherein the ceramide mixture is composed of ceramide NP, ceramide NG and ceramide AP in a mass ratio of 22.1:8-10, and the liposome membrane material is composed of hydrogenated lecithin and tricetyl phosphate in a mass ratio of 1-10:0.02-3. The technology has good salt tolerance and is used for treating skin sensitivity. However, it cannot simultaneously solve problems such as barrier damage and inflammatory cascade, thus making consumers fall into a vicious cycle and skin problems prone to recurrence. SUMMARY

[0006] The present application aims to provide a ceramide and plant extract composite synergistic repair system based on biomimetic liposomes and application thereof, and form a multi-element repair logic of biomimetic structure matching-inflammation resistance-moisture temperature regulation-activation of cell metabolism to achieve efficient skin repair.

[0007] The object of the present application can be achieved by the following technical solution: a ceramide and plant extract composite synergistic repair system based on biomimetic liposomes, comprising ceramide biomimetic liposomes, yeast rice fermentation filtrate, hamamelis extract and sugar isomer in a mass ratio of (0.1-3):(0.1-5):(0.01-0.5):(0.1-5).

[0008] The mass ratio of the ceramide biomimetic liposomes, yeast rice fermentation filtrate, hamamelis extract and sugar isomer is (0.1-1):(0.1-3):(0.15-0.3):(0.15-2).

[0009] Further, the particle size of the ceramide biomimetic liposomes is 100-150 nm.

[0010] The present application further provides an application of the ceramide and plant extract composite synergistic repair system based on biomimetic liposomes, wherein the composition is used as an efficacy component in skin care products.

[0011] Further, the skin care product comprises skin care water, serum, emulsion or cream.

[0012] Further, the skin care product further comprises a base component, and the base component comprises one or more of a humectant, a skin conditioner, a thickening regulator, an emulsifier, an ester donor, an antioxidant, a pH regulator, a preservative or a fragrance.

[0013] Further, the humectant includes one or more of sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, propylene glycol, butylene glycol, glycerin, dipropylene glycol, glycereth-26, erythritol, glyceryl acrylate / acrylic acid copolymer or glyceryl glucoside; The skin conditioning agent includes one or more of niacinamide, bisabalol, allantoin, panthenol, beta-glucan, glucose or polyglutamic acid; The thickening regulator includes one or more of carbomer, xanthan gum, butene / ethylene / styrene copolymer, acrylates / C10-30 alkyl acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, hydroxypropyl methylcellulose, acrylamide / sodium acryloyldimethyl taurate copolymer or hydrogenated microcrystalline wax; The emulsifier includes one or more of ethylene glycol distearate, polysorbate-20, polysorbate-60, polysorbate-80, cetearyl alcohol, glyceryl stearate, PEG-100 stearate, cetearyl glucoside or sorbitan stearate; The ester donor includes one or more of caprylic / capric triglyceride, isononyl isononanoate, ethylhexyl palmitate, glyceryl caprylate, squalane, bis-diglyceryl polyacyladipate-2, jojoba seed oil or shea butter; The antioxidant includes one or more of tocopherol, tocopherol acetate, ascorbic acid or alanine; The pH adjuster includes one or more of triethanolamine, citric acid, sodium citrate or arginine; The preservative includes one or more of phenoxyethanol, methyl paraben, propyl paraben or methylisothiazolinone; The fragrance includes perfume.

[0014] Further, the skin care product is an essence, including the following components in the weight percentage: Phase A: Deionized water to 100 Sodium hyaluronate 0.1~1; Butylene glycol 1~5; Glycerin 4~6; Niacinamide 0.5~2; Panthenol 1~3; Carbomer 0.1~0.5; Xanthan gum 0.05~0.15; Polysorbate-80 1~3; Squalane 3~6; Tocopheryl Acetate 0.1~0.6; B phase: Triethanolamine 0.5~1; C phase: Ceramide biomimetic liposome 0.1~3; Yeast rice fermentation filtrate 0.1~5; Hamamelis extract 0.01~0.5; Sugar isomer 0.1~5; D phase: Phenoxyethanol 0.5~1; Essence 0.1~0.5.

[0015] Further, the essence is prepared by the following method: (1) Water phase preparation: add deionized water to the reactor, then slowly add sodium hyaluronate, butylene glycol, glycerol, nicotinamide, panthenol, carbomer, xanthan gum to the reactor, heat in a water bath at 70~80℃, and continuously stir to fully dissolve the components; (2) Oil phase preparation: weigh squalane, tocopheryl acetate, and polysorbate-80 in the reactor, and stir until a transparent and uniform system is formed; (3) Water phase-oil phase mixing: slowly pour the oil phase prepared in step (2) into the water phase reactor of step (1), and stir while pouring to ensure uniform emulsification of the system; (4) When the liquid cools to 35~45℃, add ceramide biomimetic liposome, yeast rice fermentation filtrate, hamamelis extract, and sugar isomer in sequence, and stir while pouring until all the ingredients are mixed evenly; (5) Add phenoxyethanol and essence to the mixture obtained in step (4), and adjust the pH to 5.5-6.5 with triethanolamine, and finally homogenize to obtain the essence.

[0016] The stirring speed is 200-500rpm, and the duration is 10~30 minutes.

[0017] Compared with the prior art, the present application has the following beneficial effects: (1) The present application utilizes the biological similarity characteristics of liposomes, using biomimetic liposomes as a wrapping and penetration medium, thereby promoting the expression of skin barrier proteins and achieving efficient skin repair process. In addition, plant complexes and other active ingredients also have anti-inflammatory and redness-reducing, moisturizing and water-locking effects. These components are used in synergy to form a multi-element repair logic of biomimetic structure matching-inflammation resistance-moisture temperature regulation-activation of cell metabolism.

[0018] (2) The ceramide biomimetic liposome and plant complex are used as the core component of the repair system, the active ingredients are wrapped by using the similarity between the ceramide biomimetic liposome and the cell membrane structure, efficient delivery and penetration effect are realized, and finally the skin is repaired from multiple dimensions.

[0019] Dimension one: the ceramide biomimetic liposome can promote efficient delivery and penetration of other active ingredients, and is an important component of the skin cell membrane itself, and has a great effect on skin barrier protection and repair.

[0020] Dimension two: the yeast rice fermentation filtrate is an active anti-wrinkle tightening regulating component, can activate gene regulation and collagen synthesis, and promote skin repair.

[0021] Dimension three: the hamamelis extract has anti-inflammatory and redness-reducing effects, and can improve skin problems caused by inflammation.

[0022] Dimension four: the sugar isomer contains a large number of -OH (hydroxyl) structures, can combine with water molecules through hydrogen bonds to form a cold gel network, and is used in combination with the hamamelis extract, which can not only reduce the skin surface temperature, but also keep the skin moist, and indirectly improve the skin state.

[0023] The active ingredients selected by the present application promote each other, realize synergistic effect, break through the limitations of the prior art to realize skin repair, realize improvement of the skin state from multiple dimensions, and have obvious advantages. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 The skin temperature change of the face of the subject at different times.

[0025] Figure 2 The roughness of the face of the subject at different times. DETAILED DESCRIPTION

[0026] The present application will be described in detail below in combination with the drawings and specific embodiments. The present embodiment is implemented on the premise of the technical scheme of the present application, and gives a detailed implementation mode and specific operation process, but the protection scope of the present application is not limited to the following examples.

[0027] The present application constructs a repair system with ceramide biomimetic liposome and plant complex as the core component. Its characteristics are that the ceramide biomimetic liposome realizes efficient delivery and penetration effect, and the active ingredients such as hamamelis extract, sugar isomer, yeast rice fermentation filtrate are used in combination to form a multi-dimensional repair logic of biomimetic structure matching-inflammation resistance-moisture temperature regulation-activation of cell metabolism.

[0028] Among them, the ceramide biomimetic liposome selected by the present application is commercially available from Suzhou Nakan Biotechnology Co., Ltd. and Shanghai Zhina Biotechnology Co., Ltd. The ceramide biomimetic liposome mainly includes five kinds of ceramides (NP, NS, NG, AP, EOP), cholesterols, hydrogenated lecithin, white pool rapeseed oil and other ingredients. The ceramide is a kind of lipid molecule formed by the connection of sphingosine and fatty acid through amide bond, and is a component of cell membrane. In the skin keratin layer lipid, the content of ceramide is about 40%-50%, which is composed of ceramide, cholesterols, hydrogenated lecithin, white pool rapeseed oil and other ingredients to form ceramide biomimetic liposome, and the skin cell membrane lipid bilayer is similar, which makes the ceramide biomimetic liposome have better biocompatibility with the cell membrane, is beneficial to carrying active ingredients into the cell, and improves the bioavailability of active ingredients. In addition, the particle size of the ceramide biomimetic liposome is 100-150 nm, which can more efficiently penetrate the skin keratin layer compared with other biological macromolecules, so as to penetrate the active ingredients into the skin, and then play a role.

[0029] The yeast rice fermentation filtrate is selected from commercially available products or prepared by using the published yeast rice fermentation filtrate (such as patent CN202310055025.0, etc.). The yeast rice fermentation filtrate contains various active substances, such as alpha-ketoglutaric acid, polypeptides, polysaccharides and other natural active substances, and is a core component for promoting skin barrier repair and regeneration. Some of these cell growth-related factors can enter the cell and up-regulate the expression of skin barrier-related genes, such as promoting the synthesis of filaggrin and enhancing the structure of the stratum corneum. In addition, the yeast rice fermentation filtrate can also effectively inhibit the release of cell inflammatory factors (such as IL-6), and has a repairing effect on skin damage caused by external stimuli such as ultraviolet rays, and can reduce inflammation such as redness and burning.

[0030] The hamamelis extract contains rich tannic acid, which can inhibit the release and contraction of histamine, and quickly relieve erythema and itching. In addition, the hamamelis extract can also inhibit the release of cell inflammatory factors (such as IL-6) and reduce the inflammatory reaction of the skin. The hamamelis extract used is a standardized commercially available raw material, and the tannic acid content is ≥8%. The skilled person can purchase it through multiple channels according to the published parameters.

[0031] The sugar isomer contains a large number of hydroxyl groups, which can combine with water molecules to form a cold gel network on the surface of the skin. When used in combination with the hamamelis extract (natural tannic acid), the cooling effect of the cold gel network can be enhanced. When the water in the gel evaporates slowly, it will take away the heat from the surface of the skin, producing a physical cooling effect. At the same time, tannic acid can also stimulate nerve endings to produce astringent effect and cause a short-term cooling sensation. The sugar isomer used is a standardized commercially available raw material, and the purity is ≥95% and the water content is ≤0.5%.

[0032] The synergistic effect between the above components can promote the efficient delivery and penetration of other active ingredients by the ceramide biomimetic liposome, activate gene regulation and collagen synthesis by the rice fermented filtrate of yeast, promote skin repair, improve skin problems caused by inflammation by the hamamelis extract, and indirectly improve the skin condition by maintaining skin moisture and reducing skin surface temperature when the saccharide isomerate is used in combination with the hamamelis extract, thereby jointly constructing a multi-protection system of biomimetic structure matching-inflammation resistance-moisture temperature regulation-activated cell metabolism.

[0033] The above efficacy component of the present application can be used in various skin care products, including but not limited to skin care water, serum, emulsion, cream, etc. In preparation, in addition to the above composition, the skin care product also includes the base component necessary for the preparation of various skin care products. The base component can be selected from conventional base components of various products in the prior art, and the skin care product preparation method can be prepared according to the conventional method in the prior art.

[0034] For example, the ceramide based on biomimetic liposome and the plant extract complex synergistic repair system of the present application is configured together with the base component into a repair serum, and the specific formula is shown in Table 1 below: Table 1: Serum formula proportion table The following will be described in detail through specific examples and comparative examples.

[0035] Example 1 A ceramide based on biomimetic liposome and plant extract complex synergistic repair system is composed of the following components: ceramide biomimetic liposome 1 part, rice fermented filtrate of yeast 3 parts, hamamelis extract 0.3 parts, saccharide isomerate 2 parts.

[0036] Example 2 A ceramide based on biomimetic liposome and plant extract complex synergistic repair system is composed of the following components: ceramide biomimetic liposome 3 parts, rice fermented filtrate of yeast 5 parts, hamamelis extract 0.5 parts, saccharide isomerate 5 parts.

[0037] Example 3 A ceramide based on biomimetic liposome and plant extract complex synergistic repair system is composed of the following components: ceramide biomimetic liposome 0.1 part, rice fermented filtrate of yeast 0.1 part, hamamelis extract 0.15 part, saccharide isomerate 0.15 part.

[0038] Comparative Example 1 The composition is composed of the following ingredients: ceramide biomimetic liposome 0 parts, yeast rice fermentation filtrate 0.2 parts, witch hazel extract 0.15 parts, saccharide isomerate 0.15 parts.

[0039] Comparative Example 2 The composition is composed of the following ingredients: ceramide biomimetic liposome 0.2 parts, yeast rice fermentation filtrate 0 parts, witch hazel extract 0.15 parts, saccharide isomerate 0.15 parts.

[0040] Comparative Example 3 The composition is composed of the following ingredients: ceramide biomimetic liposome 0.15 parts, yeast rice fermentation filtrate 0.15 parts, witch hazel extract 0 parts, saccharide isomerate 0.2 parts.

[0041] Comparative Example 4 The composition is composed of the following ingredients: ceramide biomimetic liposome 0.15 parts, yeast rice fermentation filtrate 0.15 parts, witch hazel extract 0.2 parts, saccharide isomerate 0 parts.

[0042] Blank Comparative Example The composition of the present application is composed of the following ingredients: ceramide biomimetic liposome 0 parts, yeast rice fermentation filtrate 0 parts, witch hazel extract 0 parts, saccharide isomerate 0 parts.

[0043] The compositions obtained in each example and comparative example were subjected to in vitro cell experiments, specifically: HaCaT human keratinocyte inflammatory factor inhibition effect test: 1) Materials and reagents ① HaCaT human keratinocytes ② The compositions obtained in Examples 1-3, Comparative Examples 1-4 and the blank comparative example were dissolved in deionized water, and the volume was adjusted to 10 mL to prepare samples; ③ IL-6 ELISA kit (containing coating antibody, detection antibody, standard, enzyme-labeled second antibody, substrate solution TMB, termination solution, washing solution, etc.), phosphate buffer solution (PBS), fetal bovine serum (FBS); 2) Instruments and equipment ELISA plate, ELISA reader (450 nm wavelength), pipette, centrifuge, constant temperature incubator (37℃); 3) Experimental principle HaCaT human keratinocytes release inflammatory factors such as IL-6 after being stimulated by external stimuli. The specific antibodies can bind to the inflammatory factors, and then the antibodies labeled with horseradish peroxidase (HRP) (secondary antibodies) can bind to the antigen-antibody complex to form a sandwich structure. After adding a specific substrate TMB, an enzyme-catalyzed reaction is activated to produce a blue complex. The absorbance value can be detected at 450 nm using an enzyme-labeled instrument. The more inflammatory factors released, the more specific antibodies bound, and the more enzymes involved in the substrate enzyme-catalyzed reaction, the greater the absorbance value.

[0044] 4) Experimental method ① HaCaT human keratinocytes were seeded in a 96-well plate at a density of 10 3 -10 4 cells / well, and 200 μL of DMEM medium was added. The plate was incubated in a 37°C, 5% CO2 incubator for 24 hours.

[0045] ② 20 μL of the samples prepared in Examples 1-3, Comparative Examples 1-4, and the blank control were added to the plate, with 3 replicates per group. The plate was incubated in the incubator for 24 hours.

[0046] ③ The cell culture supernatant was collected, and the ELISA kit was used. The supernatant of Examples 1-3, Comparative Examples 1-4, and the blank control was added to the corresponding reaction wells of the ELISA plate, and the processes of coating antibody, washing plate, blocking, adding sample, washing plate, adding detection antibody, adding enzyme-labeled secondary antibody, adding substrate for color development, stopping reaction, and reading were performed according to the kit operation instructions.

[0047] ④ Taking the blank control as a reference, the inhibition rate of IL-6 synthesis of Examples 1-3 and Comparative Examples 1-4 was calculated. The synthesis inhibition rate (%) = (experimental group absorbance - blank control absorbance) / blank control absorbance * 100%. The absolute value of the calculated results was taken.

[0048] 5) Experimental results According to the above experimental steps, the IL-6 inhibition rates of different experimental groups were measured as shown in the following table: Table 2 IL-6 inhibition rate As can be seen from Table 2, the compositions of Examples 1-3 all exhibit significant inhibitory effect on the inflammatory factor IL-6 of human keratinocytes, and the inhibition rate increases with the increase of the concentration of each active ingredient. The IL-6 inhibition rates of Comparative Examples 1-4 are all significantly lower than that of the complete system, and the inhibition rate of Comparative Example 3 is the lowest, which is 16.63%, indicating that the hamamelis extract plays a major role in the anti-inflammatory process, and the tannic acid and other components contained therein can effectively inhibit the release of inflammatory factors. The inhibition rates of Comparative Example 1 and Comparative Example 2 are also low, which are 20.12% and 20.86% respectively, indicating that the ceramide biomimetic liposome as a penetration enhancer can improve the bioavailability of other active ingredients, and the active substances in the yeast rice fermentation filtrate can indirectly enhance the anti-inflammatory effect by up-regulating the expression of barrier-related genes and inhibiting the release of IL-6. The inhibition rate of Comparative Example 4 is 20.15%, which is still far lower than that of the complete system. In summary, the ceramide biomimetic liposome, the yeast rice fermentation filtrate, the hamamelis extract and the saccharide isomerate synergistically inhibit the release of the inflammatory factor IL-6, and have an anti-inflammatory effect.

[0049] Application Example The composition containing ceramide biomimetic liposome and plant complex is configured together with the matrix components into a repair-type skin care product, specifically a serum, and the content of each component is as follows: Table 3. Serum formulation ratio table, content / wt% Table 3 (continued) Note: The amount of each component in the above table is the mass percentage, and can be obtained through the market channel.

[0050] The difference between Application Examples 1-3 is that the content of each active ingredient is different, the difference between Application Comparative Examples 1-14 and Application Example 3 is that one or several key active ingredients are missing, and the difference between the blank example and Example 3 is that the blank comparative example lacks all active ingredients.

[0051] The preparation method of the serum is as follows: (1) Water phase preparation: take a certain amount of deionized water in a reactor, then weigh the sodium hyaluronate, butylene glycol, glycerol, nicotinamide, panthenol, carbomer, and xanthan gum according to the formulation ratio, slowly add them into the reactor, heat in a water bath at 70-80°C, and continuously stir at a speed of 300 rpm for 5 minutes, so that each component is fully dissolved.

[0052] (2) Oil phase preparation: weigh squalane, tocopheryl acetate, and polysorbate-80 in a reactor according to the formulation ratio, and stir until a transparent and uniform system is formed.

[0053] (3) Water phase-oil phase mixing: slowly pour the oil phase prepared in step (2) into the water phase reactor of step (1), stirring at the same time, speed 200-500 rpm, for 20 minutes, to ensure uniform emulsification of the system (no stratification, no oil droplets).

[0054] (4) When the liquid obtained in step (3) is cooled to about 40°C, the corresponding mass of ceramide precise biomimetic liposome, yeast rice fermentation filtrate, witch hazel extract, and sugar isomer is added in sequence, stirring at the same time, speed 300 rpm, for 5 minutes, until all ingredients are mixed evenly.

[0055] (5) Add phenoxyethanol and essence to the mixture, and adjust the pH to 5.5-6.5 with triethanolamine, and then homogenize to obtain the serum.

[0056] The serum obtained in each of the above application examples and comparative examples is subjected to performance testing, as follows: (I) Human efficacy evaluation experiment (1) Test samples: serums prepared from the components of application examples 1-3, application comparative examples 1-14, and application blank example described in Table 3; (2) Test subjects: the research subjects are 54 healthy people with sensitive skin, aged between 30 and 60 years old, all of whom have sensitive skin. Before entering the group, the subjects are asked a series of questions about disease history, health status, etc. according to the inclusion and exclusion criteria, and the skin of the test site is evaluated for compliance and skin color test screening to ensure that the test results are effective and reliable.

[0057] (3) Test method: ① Divide the subjects into nine groups at random, with three people in each group, and use the same prepared serum for the subjects in the same group.

[0058] ② The subjects complete facial cleaning at the test center, and then sit quietly for 30 minutes before taking VISIA pictures and testing skin redness with a skin color meter, testing facial skin stratum corneum water content with a non-invasive skin tester, and testing skin roughness in a smiling state with an Antera 3D instrument.

[0059] ③ Then, each group member uses the corresponding test serum, and after 30 minutes, takes VISIA pictures and tests skin redness with a skin color meter, tests facial skin stratum corneum water content with a non-invasive skin tester, and tests skin roughness in a smiling state with an Antera 3D instrument.

[0060] ④ In addition, after using the serum for 14 days and 28 days, VISIA pictures are taken again, and skin redness is tested with a skin color meter, facial skin stratum corneum water content is tested with a non-invasive skin tester, and skin roughness in a smiling state is tested with an Antera 3D instrument.

[0061] ⑤Cooling effect test: The subjects complete facial cleansing at the test center, sit quietly for 30 min, wear a hot compress mask for 15 min, and then use a non-invasive skin test instrument to test the facial skin temperature. Then, each group randomly uses the corresponding test serum on half of the face, immediately uses a non-invasive skin test instrument to test the facial skin temperature, and then measures the facial temperature again after 15 min.

[0062] Test instructions: ① Environmental conditions: The test is conducted in an environment with a temperature of 21±1°C and a relative humidity of 50±10% RH. Visual assessment is conducted under constant lighting (daylight lamp tubes or LED lighting with a color temperature of 5500-6500K). The subjects must adapt to this environmental condition for at least 30 minutes before the assessment and test can be conducted.

[0063] ② VISIA image capture and skin color instrument test: The skin redness can be converted into specific numerical values (EI values) through optical principles and color models, with a range of 0-100. The larger the EI value, the redder the facial skin color.

[0064] ③ Facial skin stratum corneum water content: The non-invasive skin test instrument can reflect the water content in the skin by detecting the conductivity of the uppermost layer of the skin.

[0065] ④ Skin temperature measurement principle: The infrared detector receives the infrared radiation of each area of the skin, and through specific calculations, it is converted into a temperature value and generates a visual temperature distribution map.

[0066] ⑤ Antera 3D can collect detailed information of the human face, form a 3D image and selected filters, analyze the texture of the skin surface through software, and calculate the roughness value of the skin using algorithms. The larger the value, the rougher the skin.

[0067] ⑥ Test results can be calculated using the following formula: Change rate = (analysis value after using the product - analysis value before using the product) ÷ analysis value before using the product x 100%, and the absolute value of the calculation result is taken. The change rate of the same group is averaged.

[0068] Test results 1) Anti-inflammatory and redness reduction effect According to the above experimental procedures, the subjects were tested for skin redness using VISIA and skin color instrument at different times, and the degree of facial redness was reflected by the specific numerical value (EI value). The data of each group were statistically calculated, and the following results were obtained: Table 4 Facial redness changes of subjects in different groups at different times From Table 4, it can be seen that the compositions of application examples 1-3 have better anti-inflammatory and redness-reducing repair effects, and the redness-reducing effect is more obvious with the increase of the concentration of each effective component. After using the serum for 30 min, the redness of the face of each group is relatively close except for the blank control, which may be due to the short interval time and the difference in the effect of the components. However, after using the serum for 28 days, application example 3 and application examples 1-14 show obvious differences, which also reflects the advantage of the selected composition in skin repair. In addition, by comparing application example 3 and comparative examples 1-4, it can be found that when the composition lacks the carbohydrate isomer, the redness change rate of the facial skin is slightly lower than that of application example 3, when the composition lacks the ceramide biomimetic liposome or the yeast rice fermentation filtrate, the redness change rate of the facial skin is greatly different from that of application example 3, and when the composition lacks the hamamelis extract, the redness change rate of the facial skin is the most different from that of application example 3, which shows that the hamamelis extract plays a major role in skin redness reduction, and the specific reason is that the hamamelis extract has the effect of converging blood vessels. By observing application example 3 and application examples 11-14, it can be found that the anti-inflammatory and redness-reducing effect of the effective components used alone is much lower than that of application example 3 on the 28th day of use. By observing application example 3 and application examples 5-10, it can be found that the anti-inflammatory and redness-reducing effect of the two effective components used together is lower than that of application example 3 on the 28th day of use, but is higher than that of the two components used alone. By observing application example 3 and application examples 1-4, it can be found that the anti-inflammatory and redness-reducing effect of the three effective components used together is lower than that of application example 3 on the 28th day of use, but is higher than that of the three components used alone. By comprehensively observing the results of application example 3 and application examples 1-14, it can be found that the four effective components used together have better synergistic anti-inflammatory and redness-reducing effect.

[0069] 2) Moisturizing repair effect According to the above experimental procedure, the non-invasive skin tester was used to test the water content of the stratum corneum of the facial skin of the subjects at different times, and compared with the water content of the skin before use, and the data of each group was statistically calculated to obtain the following results: Table 5 Change of facial water content of subjects in each group at different times From Table 5, it can be seen that the compositions of application examples 1-3 have better moisturizing and water-locking effects, and the effect is more obvious with the increase of the concentration of each effective component. By comparing application example 1 and application comparative example 3, it can be found that the change rate of facial water content of application comparative example 1 lacking ceramide biomimetic liposomes is significantly less than that of application example 3, that is, the moisturizing effect of application example 3 containing four kinds of effective components is better, which shows that ceramide biomimetic liposomes have important role in barrier protection and moisturizing repair. By observing application example 3 and application comparative example 4, there is a large difference in the change rate of water content, which shows that the sugar isomer also has certain moisturizing and water-locking effect. In addition, the change rate of skin water content of application comparative examples 2 and 3 is slightly lower than that of application example 3, which shows that the effect of yeast rice fermentation filtrate and witch hazel extract on moisturizing and water-locking is limited. By observing application example 3 and application comparative examples 11-14, it can be found that the change rate of skin water content when using the effective components alone is much lower than that of application example 3 on the 28th day. By observing application example 3 and application comparative examples 5-10, it can be found that the change rate of skin water content when using two kinds of effective components together is lower than that of application example 3 on the 28th day, but is higher than the moisturizing effect when using two kinds of components alone. By observing application example 3 and application comparative examples 1-4, it can be found that the change rate of skin water content when using three kinds of effective components together is lower than that of application example 1 on the 28th day, but is higher than the moisturizing effect when using three kinds of components alone. By comprehensively observing the results of application example 3 and application comparative examples 1-14, it can be found that four kinds of effective components together have better synergistic moisturizing effect.

[0070] ③Cooling effect According to the above experimental steps, the skin temperature of the subjects was tested by a non-invasive skin tester at different times, and compared with the skin temperature before use. The data of each group was statistically calculated to obtain the following results: Table 6 Change of facial skin temperature of subjects in each group at different times From Table 6, it can be seen that the compositions of application examples 1-3 have better cooling effect, and the effect is more obvious with the increase of the concentration of each active ingredient. By comparing application example 3 with application comparative examples 1 and 2, it can be found that the change rate of facial skin temperature of the composition lacking the ceramide biomimetic liposome or the yeast rice fermentation filtrate is lower than that of application example 3, which indicates that the ceramide biomimetic liposome and the yeast rice fermentation filtrate have limited effect on reducing skin temperature. By observing application example 3 and application comparative example 3, it can be found that the change rate of skin temperature is different from that of application example 3 when lacking the hamamelis extract, which may be because the hamamelis extract can constrict blood vessels and assist in reducing skin temperature by regulating blood circulation. In addition, application comparative example 4 and application example 3 are quite different, which indicates that the sugar isomer has a certain effect on skin cooling. By observing application example 3 and application comparative examples 11-14, it can be found that the facial skin temperature of application comparative examples 11-14 is reduced after 15 min, but there is a large difference with application example 3, which indicates that the cooling effect of the active ingredient used alone is not as good as that of application example 3. By observing application example 3 and application comparative examples 5-10, it can be found that the change range of facial skin temperature when two active ingredients are used together is lower than that of application example 3, but higher than that when the two ingredients are used alone. By observing application example 3 and application comparative examples 1-4, it can be found that the change range of facial skin temperature when three active ingredients are used together is lower than that of application example 3, but higher than that when the three ingredients are used alone. By comprehensively observing the results of application example 3 and application comparative examples 1-14, it can be found that four active ingredients used together have better effect on reducing facial skin temperature.

[0071] ④ Anti-wrinkle firming effect Smiling causes the facial expression muscles (such as orbicularis oculi) to contract, which pulls the skin to form temporary wrinkles, and the roughness of the skin surface can exacerbate the visual depth and area of dynamic wrinkles. In this experiment, the change rate of skin roughness under smiling state was tested by Antera 3D instrument. The smaller the analysis value and the change rate, the better the improvement of skin roughness.

[0072] During the test, the subjects closed their mouths (without showing teeth), smiled to the maximum amplitude, and the skin roughness was tested by the instrument Antera 3D, and the data is shown in Table 7.

[0073] Table 7 Change of skin roughness of subjects in each group at different times From Table 7, it can be seen that the compositions of application examples 1-3 have better anti-wrinkle and skin tightening effects, and the effects are more obvious with the increase of the concentration of each active ingredient. By comparing application example 3 with application comparative examples 1 and 2, it can be found that the change rate of facial skin roughness is obviously lower when the ceramide biomimetic liposome or the yeast rice fermentation filtrate is absent, which indicates that the ceramide biomimetic liposome and the yeast rice fermentation filtrate play an important role in anti-wrinkle, skin tightening and barrier repair. By observing application example 3 and application comparative example 4, it can be found that there is a great difference in the change rate of facial skin roughness, which may be because the sugar isomer can maintain skin moisture and form a water film on the skin surface, thereby indirectly improving the skin condition. In addition, by observing application example 3 and application comparative example 3, it can be found that the change rate of facial skin roughness is lower than that of application example 3 when the witch hazel extract is absent, which indicates that the witch hazel extract has limited effect on anti-wrinkle and skin tightening, and its absence has the least effect on roughness. When each ingredient is used alone (application comparative examples 11-14), the facial skin roughness is reduced after 28 days of use, but the reduction is far lower than that of application example 3, which indicates that the anti-wrinkle and skin tightening effects of the active ingredients used alone are far lower than those of application example 3. By observing application example 3 and application comparative examples 5-10, it can be found that the change amplitude of facial skin roughness when two active ingredients are used together is lower than that of application example 3, but higher than that when each ingredient is used alone. By observing application example 3 and application comparative examples 1-4, it can be found that the change amplitude of facial skin roughness when three active ingredients are used together is lower than that of application example 3, but higher than that when each ingredient is used alone. By comprehensively observing the results of application example 3 and application comparative examples 1-14, it can be found that the four active ingredients used together have better synergistic anti-wrinkle and skin tightening effects.

[0074] In order to more intuitively show the experimental results, a set of skin temperature and skin roughness test results are attached below, wherein Figure 1 is the change of facial skin temperature of the subject at different times, Figure 1 The left graph is the facial skin temperature of the subject immediately after hot compress, and the right graph is the facial skin temperature of the subject immediately after applying the sample. In the graph, the white area is the high temperature area, the red area is the second high temperature area, and the blue area is the low temperature area.

[0075] From Figure 1 it can be seen that immediately after hot compress, the highest temperature of the facial skin of the subject is 39.7°C (marked by a blue circle in the graph), and the temperature at the nose bridge is 37.2°C (marked by a white circle in the graph). Immediately after applying the essence, the highest temperature of the facial skin of the subject is 37.3°C (marked by a blue circle in the graph), and the temperature at the nose bridge is 32.4°C (marked by a white circle in the graph). It can be seen that in several specific areas, the temperature of the facial skin of the subject decreases obviously after applying the essence.

[0076] Figure 2 The roughness of the face of the subject at different times, Figure 2 The left image in the figure is the roughness of the face of the subject without applying the essence, and the right image is the roughness of the face of the subject after applying the essence for 28 days. The blue area in the figure is the skin concave area, and the red area is the skin convex area.

[0077] From Figure 2 It can be seen that before applying the essence, the area of the blue and red areas of the face of the subject is large and widely distributed, which indicates that the subject has many wrinkles and rough skin. After applying the essence for 28 days, the area of the blue and red areas of the face of the subject is greatly reduced, and the distribution range is also obviously reduced, which indicates that the essence can effectively improve the roughness of the face of the subject and has obvious anti-wrinkle effect.

[0078] From the above performance detection, it can be seen that the ceramide biomimetic liposome, the yeast rice fermentation filtrate, the hamamelis extract and the saccharide isomerate produce a synergistic effect, and the example containing the four functional ingredients can significantly improve the skin redness, has good anti-inflammatory and redness-removing repair effect, has good moisturizing effect, and can also play an anti-wrinkle and firming effect. When one or several of the functional ingredients are missing, the effect is poor, which proves that the application forms a multi-element repair system of biomimetic structure matching-inflammation resistance-moisture temperature regulation-cell metabolism activation.

Claims

1. A ceramide and plant extract complex synergistic repair system based on biomimetic liposomes, characterized in that, The ceramide biomimetic liposome, the yeast rice fermentation filtrate, the hamamelis extract and the saccharide isomerate are in a mass ratio of (0.1-3):(0.1-5):(0.01-0.5):(0.1-5).

2. The ceramide and plant complex synergistic repair system based on biomimetic liposomes according to claim 1, characterized in that, The mass ratio of the ceramide biomimetic liposome, the yeast rice fermentation filtrate, the hamamelis extract and the saccharide isomerate is (0.1-1):(0.1-3):(0.15-0.3):(0.15-2).

3. The ceramide and plant complex synergistic repair system based on biomimetic liposomes according to claim 1, characterized in that, The particle size of the ceramide biomimetic liposome is 100-150 nm.

4. The use of the ceramide and plant extract complex synergistic repair system based on biomimetic liposomes according to any one of claims 1-3, characterized in that, The composition is used as an efficacy component in a skin care product.

5. The use of the ceramide and plant extract complex synergistic repair system based on biomimetic liposomes according to claim 4, characterized in that, The skin care product is a skin care water, an essence, a lotion or a cream.

6. The use of the ceramide and plant extract complex synergistic repair system based on biomimetic liposomes according to claim 4, characterized in that, The skin care product further comprises a base component, and the base component comprises one or more of a humectant, a skin conditioning agent, a thickening agent, an emulsifier, an ester donor, an antioxidant, a pH adjuster, a preservative or a fragrance.

7. The use of a ceramide and plant extract complex synergistic repair system based on a biomimetic liposome according to claim 6, characterized in that, The humectant comprises one or more of sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, propylene glycol, butylene glycol, glycerin, dipropylene glycol, glycereth-26, erythritol, glyceryl acrylate / acrylic acid copolymer or glyceryl glucoside. The skin conditioning agent comprises one or more of niacinamide, bisabalol, allantoin, panthenol, beta-glucan, glucose or polyglutamic acid. The thickening agent comprises one or more of carbomer, xanthan gum, butylene / ethylene / styrene copolymer, acrylates / C10-30 alkyl acrylate crosspolymer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, hydroxypropyl methylcellulose, acrylamide / sodium acryloyldimethyl taurate copolymer or hydrogenated microcrystalline wax. The emulsifier comprises one or more of ethylene glycol distearate, polysorbate-20, polysorbate-60, polysorbate-80, cetearyl alcohol, glyceryl stearate, PEG-100 stearate, cetearyl glucoside or sorbitan stearate. The ester donor comprises one or more of caprylic / capric triglyceride, isononyl isononanoate, ethylhexyl palmitate, glyceryl caprylate, squalane, bis-diglyceryl polyacyladipate-2, jojoba seed oil or shea butter. The antioxidant comprises one or more of tocopherol, tocopherol acetate, ascorbic acid or alanine. The pH adjuster comprises one or more of triethanolamine, citric acid, sodium citrate or arginine. The preservative comprises one or more of phenoxyethanol, methyl paraben, propyl paraben or methylisothiazolinone. The fragrance comprises a fragrance.

8. The use of the ceramide and plant extract complex synergistic repair system based on biomimetic liposomes according to claim 6, characterized in that, The skin care product is an essence, and comprises the following components by weight percentage: Phase A: Deionized water to 100 Sodium hyaluronate 0.1-1; Butylene glycol 1-5; Glycerin 4-6; Niacinamide 0.5-2; Panthenol 1-3; Carbomer 0.1-0.5; Xanthan gum 0.05-0.15; Polysorbate-80 1-3; Squalane 3-6; Tocopherol acetate 0.1-0.6; Phase B: Triethanolamine 0.5-1; Phase C: Ceramide biomimetic liposome 0.1~3; Yeast rice fermentation filtrate 0.1~5; Hamamelis extract 0.01~0.5; Saccharide isomerate 0.1~5; D phase: Phenoxyethanol 0.5~1; Essence 0.1~0.

5.

9. The use of a ceramide and plant extract complex synergistic repair system based on a biomimetic liposome according to claim 8, characterized in that, The essence is prepared by the following method: (1) Water phase preparation: add deionized water to the reactor, then slowly add sodium hyaluronate, butylene glycol, glycerol, nicotinamide, panthenol, carbomer, xanthan gum to the reactor, heat in a water bath at 70~80℃, and continuously stir to fully dissolve each component; (2) Oil phase preparation: weigh squalane, tocopherol acetate, and polysorbate-80 in the reactor, and stir until a transparent and uniform system is formed; (3) Water phase-oil phase mixing: slowly pour the oil phase prepared in step (2) into the water phase reactor of step (1), and stir while pouring to ensure uniform emulsification of the system; (4) When the liquid cools to 35~45℃, add ceramide biomimetic liposome, yeast rice fermentation filtrate, hamamelis extract, and saccharide isomerate in sequence, and stir while pouring until all ingredients are mixed evenly; (5) Add phenoxyethanol and essence to the mixture obtained in step (4), and adjust the pH to 5.5-6.5 with triethanolamine, and finally homogenize to obtain the essence.

10. The use of the ceramide and plant extract complex synergistic repair system based on biomimetic liposomes according to claim 8, characterized in that, The stirring speed is 200-500 rpm, and the duration is 10~30 minutes.

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