A lung cancer rapid detection system based on femtosecond laser and Raman spectrum analysis

Abstract

The application discloses a lung cancer rapid detection system based on femtosecond laser and Raman spectrum analysis, and relates to the technical field of spectrum diagnosis equipment, and comprises a substrate manufacturing module, a sample loading module and a detection module, wherein the substrate manufacturing module comprises a femtosecond laser, a half-wave plate, a Gartaieller prism, a filter, a shutter, a light source, a monitor, a lens, a beam splitter, a dichroic mirror, a microscope objective and a metal substrate, and is used for manufacturing nano microstructures on the metal substrate under the femtosecond laser. The metal substrate of the application constructs the super-hydrophobic micro-nano structure on the metal surface by the femtosecond laser in one step, has the abilities of SERS enhancement and instant body fluid molecule enrichment, realizes detection integration, is non-invasive in the whole process, has high sensitivity, high specificity, low cost, can be portable into bedside or intraoperative, and provides an in-situ, non-invasive, low-cost and second-level discrimination new tool for early screening, curative effect monitoring and incisal edge evaluation of high-risk groups.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of spectral diagnosis equipment, in particular to a lung cancer rapid detection system based on femtosecond laser and Raman spectrum analysis. BACKGROUND

[0002] Small cell lung cancer (SCLC) progresses fast and is easy to metastasize, and a bedside detection technology that can realize "second-level" discrimination before biopsy is urgently needed in clinical practice; existing serum markers, imaging and electron microscopy biopsy have bottlenecks such as low sensitivity, long cycle and high false negative rate, and conventional SERS substrates are difficult to be directly used for trace exhaled air or pleural effusion samples due to droplet spreading and poor signal repeatability. Femtosecond laser can construct super-hydrophobic micro-nano structures on the metal surface in one step, can instantly capture and enrich target molecules, can significantly improve the Raman enhancement factor, and can realize the collection of SERS fingerprints at the level of body fluid and single cell; combined with a portable Raman probe, specific waveform discrimination of SCLC can be quickly completed, with high sensitivity and strong specificity, providing an in-situ, non-invasive and low-cost new path for early screening of high-risk groups and rapid evaluation of intraoperative margins.

[0003] Femtosecond laser is a kind of ultrashort pulse laser with extremely high spatial resolution and extremely small heat affected zone, and is widely used in micro-nano processing. The microstructure manufactured on the metal surface can be used to enhance the Raman signal (i.e. surface enhanced Raman scattering, SERS) and improve the detection sensitivity. Raman spectrum is a non-invasive and label-free spectral analysis technology that can reflect the molecular structure information of the tissue. There is a significant difference in Raman spectral characteristics between cancerous lung tissue and normal lung tissue, and it has the potential for rapid, non-invasive and molecular-level diagnosis. SUMMARY

[0004] To achieve the above object, the present application provides the following technical scheme: a lung cancer rapid detection system based on femtosecond laser and Raman spectrum analysis, comprising a substrate manufacturing module, a sample loading module and a detection module.

[0005] The substrate manufacturing module comprises a femtosecond laser, a half-wave plate, a Gartler prism, a filter, a shutter, a light source, a monitor, a lens, a beam splitter, a dichroic mirror, a microscope objective and a metal substrate. The substrate manufacturing module is used to manufacture nano microstructure on the metal substrate under the femtosecond laser.

[0006] The sample loading module comprises a conveying table, a motor and a sample dropper.

[0007] The detection module comprises a microscope, a light mirror assembly, a laser and a grating light source module. The light mirror assembly is used to superimpose the light paths of the laser and the grating light source module and then irradiate the metal substrate through the microscope.

[0008] As preferred, the light mirror assembly comprises light mirror one, light mirror two, light mirror three, light mirror four and light mirror five, the light mirror four irradiates the light path of the grating light source module to the light mirror five, the light mirror three irradiates the light path of the laser to the light mirror five, the light paths of the grating light source module and the laser are superimposed at the light mirror five and then irradiate the metal substrate through the microscope.

[0009] As preferred, the grating light source module comprises a refrigeration unit and a light source emitter.

[0010] The present application has the following advantages:

[0011] The present application can complete the tissue sample detection at one time, and is non-invasive throughout; the metal substrate is one-step formed, does not need photoresist, is environmentally friendly and efficient; the prepared super-hydrophobic surface can inhibit protein adsorption and reduce background fluorescence; the metal substrate with nano microstructure is used once, which can avoid cross contamination; Raman detection can realize early rapid screening of malignant tumors. The metal substrate is constructed with a super-hydrophobic micro-nano structure on the metal surface by a femtosecond laser, has the ability of SERS enhancement and instant enrichment of body fluid molecules, realizes detection integration; has high sensitivity, strong specificity and low cost, and can be portable into the bedside or intraoperative, provides an in-situ, non-invasive and low-cost second-level discrimination new tool for early screening, efficacy monitoring and incisal edge evaluation of high-risk groups. BRIEF DESCRIPTION OF DRAWINGS

[0012] Figure 1 It is a schematic diagram of the overall structure of the present application;

[0013] Figure 2 It is a schematic diagram of the structure of the femtosecond laser, the monitor and the microscope objective lens in the present application;

[0014] Figure 3 It is a schematic diagram of the structure of the sample loading module in the present application;

[0015] Figure 4 It is a schematic diagram of the structure of the detection module in the present application.

[0016] In the figure: 1, femtosecond laser; 2, half-wave plate; 3, G-Lens; 4, filter; 5, shutter; 6, light source; 7, monitor; 8, lens; 9, beam splitter; 10, dichroic mirror; 11, microscope objective lens; 12, metal substrate; 13, transfer table; 14, motor; 15, sample dropper; 16, microscope; 17, light mirror one; 18, light mirror two; 19, light mirror three; 20, light mirror four; 21, laser; 22, light mirror five; 23, grating light source module. DETAILED DESCRIPTION

[0017] Clearly, the embodiments described are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative effort belong to the scope of protection of the present application.

[0018] The present application will be further described in detail below according to the drawings and embodiments.

[0019] As shown in the drawings, Figures 1 to 4 a lung cancer rapid detection system based on femtosecond laser and Raman spectrum analysis, including a substrate manufacturing module, a sample loading module and a detection module, the substrate manufacturing module, the sample loading module and the detection module are electrically connected with an external controller;

[0020] As shown in the drawings, Figure 2 and Figure 3 The substrate manufacturing module is used to make nano microstructure on the metal substrate 12 under femtosecond laser, including femtosecond laser 1, half-wave plate 2, Gouger-Lens 3, filter 4, shutter 5, light source 6, monitor 7, lens 8, beam splitter 9, dichroic mirror 10, microscope objective 11 and metal substrate 12;

[0021] As shown in the drawings, Figure 3 The sample loading module includes a conveying table 13, a motor 14 and a sample dropper 15, and the conveying table 13 is used to convey the metal substrate 12;

[0022] As shown in the drawings, Figure 4 The detection module is used to perform Raman detection on the tissue sample carried by the metal substrate 12 to analyze whether it is diseased, and the detection module includes a microscope 16, an optical lens assembly, a laser 21 and a grating light source module 23, the optical lens assembly is used to superimpose the light paths of the laser 21 and the grating light source module 23 and then irradiate them to the metal substrate 12 through the microscope 16; the grating light source module 23 includes a refrigeration unit and a light source emitter.

[0023] The optical lens assembly includes optical lens one 17, optical lens two 18, optical lens three 19, optical lens four 20 and optical lens five 22, the optical lens four 20 irradiates the light path of the grating light source module 23 to the optical lens five 22, the optical lens three 19 irradiates the light path of the laser 21 to the optical lens five 22, and the light paths of the grating light source module 23 and the laser 21 are superimposed at the optical lens five 22 and then irradiate them to the metal substrate 12 through the microscope 16.

[0024] The working principle of all the above embodiments is as follows:

[0025] The laser emitted by the femtosecond laser 1 passes through the half-wave plate 2, the Gartler prism 3, the filter 4 and the fast opening and closing of the shutter 5 in turn and enters the dichroic mirror 10; the monitoring light emitted by the monitor 7 passes through the lens 8 and, together with the visible light emitted by the light source 6, enters the dichroic mirror 10 through the beam splitter 9; the combined light formed after the laser, the monitoring light and the visible light enter the dichroic mirror 10 enters the microscope objective 11 to make microstructure on the metal base 12;

[0026] After the surface nanometer microstructure of the metal base 12 is completed, the motor 14 drives the conveying table 13 to rotate, and the metal base 12 on which the surface nanometer microstructure is completed is conveyed to the lower side of the sample dropper 15, the sample dropping operation is completed, and the metal base 12 is continuously conveyed to the detection module;

[0027] After the metal base 12 moves to the position corresponding to the microscope 16, the conveying table 13 stops rotating, the light path of the grating light source module 23 and the laser 21 is superimposed on each other through the optical lens assembly and then irradiates the tissue sample carried by the metal base 12 through the microscope 16, so that the Raman detection is carried out, and whether the tissue sample is diseased is analyzed.

[0028] In summary, the tissue sample detection can be completed at one time, and the whole process is non-invasive; the metal base 12 is formed in one step, and no photoresist is needed, which is environmentally friendly and efficient; the super-hydrophobic surface formed can inhibit protein adsorption and reduce background fluorescence; the metal base 12 with nanometer microstructure is used once, which can avoid cross contamination; the Raman detection can realize early rapid screening of malignant tumors.

[0029] The metal base 12 is constructed with super-hydrophobic micro-nano structure on the metal surface by femtosecond laser, has the ability of SERS enhancement and instant enrichment of body fluid molecules, realizes detection integration, has high sensitivity, strong specificity and low cost, can be portable into bedside or intraoperative, provides in-situ, non-invasive and low-cost second-level discrimination new tools for early screening, efficacy monitoring and incisal margin evaluation of high-risk groups.

[0030] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A rapid lung cancer detection system based on femtosecond laser and Raman spectroscopy analysis, characterized in that, Includes a substrate manufacturing module, a sample loading module, and a testing module; The substrate fabrication module includes a femtosecond laser (1), a half-wave plate (2), a GlanTeller prism (3), a filter (4), a shutter (5), a light source (6), a monitor (7), a lens (8), a beam splitter (9), a dichroic mirror (10), a microscope objective (11), and a metal substrate (12). The substrate fabrication module is used to fabricate nanostructures on the metal substrate (12) under femtosecond laser. The laser emitted by the femtosecond laser (1) passes through the half-wave plate (2), the GlanTeller prism (3), and the filter (4) in sequence, and enters the dichroic mirror (10) through the rapid opening and closing of the shutter (5). At the same time, the monitoring light emitted by the monitor (7) passes through the lens (8) and enters the dichroic mirror (10) together with the visible light emitted by the light source (6) through the beam splitter (9). The combined light formed by the laser, monitoring light, and visible light entering the dichroic mirror (10) enters the microscope objective (11) to fabricate microstructures on the metal substrate (12). The sample loading module includes a conveyor (13), a motor (14), and a sample dispenser (15). The detection module includes a microscope (16), an optical mirror assembly, a laser (21) and a grating light source module (23). The optical mirror assembly is used to superimpose the optical paths of the laser (21) and the grating light source module (23) and then irradiate the metal substrate (12) through the microscope (16).

2. The rapid lung cancer detection system based on femtosecond laser and Raman spectroscopy analysis according to claim 1, characterized in that, The optical mirror assembly includes optical mirror one (17), optical mirror two (18), optical mirror three (19), optical mirror four (20) and optical mirror five (22). Optical mirror four (20) illuminates the optical path of the grating light source module (23) onto optical mirror five (22), and optical mirror three (19) illuminates the optical path of the laser (21) onto optical mirror five (22). The optical paths of the grating light source module (23) and the laser (21) are superimposed on optical mirror five (22) and then illuminated onto the metal substrate (12) by microscope (16).

3. The rapid lung cancer detection system based on femtosecond laser and Raman spectroscopy analysis according to claim 2, characterized in that, The grating light source module (23) includes a cooling unit and a light source emitter.

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