Biological bottom modification composition, preparation as well as preparation method and application of biological bottom modification composition

Abstract

The invention relates to the technical field of biology, in particular to a biological substrate modification composition, a preparation as well as a preparation method and application of the biological substrate modification composition. The biological substrate modification composition provided by the invention is prepared from the following components: bacillus subtilis YXSY-01 bacterial powder, sphingomonas aquatic bacteria HD-01 bacterial liquid, bentonite, oyster shell powder, attapulgite, zeolite powder, fish bone meal of raw fish and water. The biological substrate modification composition is prepared from the following raw materials in parts by weight: 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch, 20-30% of corn starch and 20-30% of corn starch. The biological substrate modification composition and the preparation provided by the invention have the capabilities of degrading faeces of bred animals and reducing the content of nitrite. The composition and the preparation can reduce the accumulation of excrement of cultured animals in a culture pond, reduce the nitrite content, purify the water quality of the culture pond and reduce the morbidity of the cultured animals.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to a biomodified composition, formulation, preparation method and application thereof. Background Technology

[0002] In aquaculture, aquatic animals have low feed utilization rates; only a small portion of the fed feed is effectively utilized, with the majority being excreted as feces. The greater the amount of feces produced, the more significant the adverse impact on the aquaculture water. The amount of feces in aquaculture water is one of the key factors determining the quality of the aquatic environment. Currently, aquaculture is developing towards high-density and intensive factory-style farming. This method involves artificially controlling each stage of the farming process based on high-density farming to achieve high yields and efficiency. However, due to the high stocking density and large feeding amounts, a large amount of feces is generated during the farming process, significantly increasing the pollution pressure on the water body. This leads to severe water pollution, disrupts the ecological balance of ponds, and consequently causes the proliferation of pathogens and outbreaks of diseases in farmed animals.

[0003] In the later stages of shrimp farming in small sheds, the excessive use of high-protein feed leads to obstructed nitrogen cycling, resulting in a large accumulation of inorganic nitrogen sources in the water. This causes excessively high nitrite levels, severely impacting the health of aquatic animals and causing significant losses to the farm. Currently, common methods for removing nitrite from water include physical, chemical, and biological methods. Physical methods mainly utilize physical adsorption and water exchange, but these methods are slow to take effect, prone to causing stress, and cannot fundamentally solve the problem. Chemical methods for removing nitrite mainly use chemical barrier agents and strong oxidants, but they are difficult to effectively reduce nitrite levels. Biological methods mainly utilize nitrifying and denitrifying bacteria to gradually convert nitrite into nitrogen gas, but this method is characterized by slow growth rates, low treatment efficiency, and difficulty in preservation during practical use.

[0004] Therefore, there is an urgent need for a method that can effectively solve the problem of animal fecal accumulation and degrade nitrite at the same time. Summary of the Invention

[0005] The purpose of this invention is to provide a biological bottom-improving composition, formulation, preparation method, and application thereof to solve the problems existing in the prior art. The biological bottom-improving granules provided by this invention have the ability to degrade farmed animal feces and reduce nitrite content, which can reduce the accumulation of farmed animal feces in aquaculture ponds, purify the water quality of aquaculture ponds, and ultimately reduce the incidence of disease in farmed animals.

[0006] To achieve the above objectives, the present invention provides the following solution:

[0007] This invention provides a bio-based substrate modification composition comprising the following components in parts by weight:

[0008] 0.3-1.7 parts Bacillus subtilis YXSY-01 bacterial powder, 0.2-2 parts Sphingomonas aquatilis HD-01 bacterial solution, 30-50 parts bentonite, 30-40 parts oyster shell powder, 1-3 parts attapulgite, 2-6 parts zeolite powder, 1-1.5 parts raw fish bone meal, and 1.5-4.5 parts water;

[0009] The preservation number of Bacillus subtilis YXSY-01 is GDMCC No: 64078; the preservation number of Sphingomonas aquaticus HD-01 is GDMCC No: 64330.

[0010] Preferably, the bio-modified bottom composition comprises the following components in parts by weight:

[0011] 1 part Bacillus subtilis YXSY-01 bacterial powder, 1.5 parts aquatic sphingomonas HD-01 bacterial solution, 45 parts bentonite, 40 parts oyster shell powder, 3 parts attapulgite, 4 parts zeolite powder, 1 part raw fish bone powder and 4.5 parts water.

[0012] Alternatively, 1 part Bacillus subtilis YXSY-01 bacterial powder, 1.5 parts aquatic sphingomonas HD-01 bacterial solution, 43 parts bentonite, 40 parts oyster shell powder, 3 parts attapulgite, 5.5 parts zeolite powder, 1.5 parts raw fish bone powder, and 4.5 parts water.

[0013] Preferably, the viable count of Bacillus subtilis YXSY-01 in the Bacillus subtilis YXSY-01 powder is 1.0 × 10⁻⁶. 10 ~5.0×10 10 CFU / g;

[0014] The viable count of *Sphingomonas aquaticus* HD-01 in the bacterial culture was 1.0 × 10⁻⁶. 7 ~2.0×10 8 CFU / g;

[0015] The oyster shell powder is oyster shell powder with a diameter of 2-3 mm.

[0016] This invention provides the application of the above-described bio-bottom modification composition in the preparation of bio-bottom modification formulations.

[0017] The present invention provides a biological bottom modification formulation comprising the above-described biological bottom modification composition.

[0018] Preferably, the dosage form of the biological bottom-modifying agent is granules.

[0019] This invention provides a method for preparing the above-mentioned biological bottom-modifying agent, comprising the following steps:

[0020] The mixture of Bacillus subtilis YXSY-01 bacterial powder and Sphingomonas aquaticis HD-01 bacterial solution is mixed with the oyster shell powder to obtain a mixture;

[0021] The mixture is combined with the bentonite, the zeolite powder, the raw fish bone powder, the attapulgite clay, and the water to obtain the biological bottom modifier.

[0022] The present invention provides the application of the above-mentioned biological bottom improvement composition or the above-mentioned biological bottom improvement agent in the degradation of feces and / or reduction of nitrite content in water bodies.

[0023] The present invention provides a method for degrading feces and / or reducing the nitrite content in water, comprising the step of applying the above-mentioned biological bottom conditioner to the water.

[0024] Preferably, the application rate of the biological bottom improvement agent is 1 kg / time / acre; the application interval is 5 days.

[0025] The present invention discloses the following technical effects:

[0026] The biological bottom conditioner composition provided by this invention comprises Bacillus subtilis YXSY-01 bacterial powder, Sphingomonas aquatilis HD-01 bacterial solution, bentonite, oyster shell powder, attapulgite clay, zeolite powder, fish bone meal, and water. The biological bottom conditioner composition and formulation provided by this invention have the ability to degrade farmed animal feces and reduce nitrite content. This composition and formulation can reduce the accumulation of farmed animal feces in aquaculture ponds, lower nitrite content, purify the water quality of aquaculture ponds, and ultimately reduce the incidence of disease in farmed animals. Detailed Implementation

[0027] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0028] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0029] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0030] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0031] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0032] Unless otherwise specified, all components used in this invention are commonly purchased by those skilled in the art; all methods used in this invention are methods well known to those skilled in the art.

[0033] Example 1

[0034] A type of bio-based bottom-modifying granule has the following formula (i.e., the raw materials required to produce 1 kg of bio-based bottom-modifying granules):

[0035] 10g of Bacillus subtilis YXSY-01 bacterial powder, 15g of Sphingomonas aquaticis HD-01 bacterial solution, 450g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 40g of zeolite powder, 10g of raw fish bone powder, and 45g of purified water.

[0036] Bacillus subtilis YXSY-01 was deposited on November 27, 2023, at the Guangdong Provincial Center for Microbial Culture Collection, located at the Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, with accession number GDMCC No: 64078.

[0037] Sphingomonas aquatilis HD-01 was deposited on May 8, 2024, at the Guangdong Provincial Center for Microbial Culture Collection, located at the Institute of Microbiology, Guangdong Academy of Sciences, 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, with accession number GDMCC No: 64330.

[0038] The preparation method of Bacillus subtilis YXSY-01 bacterial powder is as follows:

[0039] (1) Preparation of Bacillus subtilis YXSY-01 fermentation broth: Bacillus subtilis YXSY-01 fermentation broth was obtained according to the preparation method of the patent with publication number CN118703394B;

[0040] (2) Preparation of Bacillus subtilis YXSY-01 bacterial powder: 10wt% dicalcium phosphate was added according to the weight of Bacillus subtilis YXSY-01 fermentation broth, and spray drying was carried out. The inlet air temperature of the spray drying was 156℃ and the outlet air temperature was 105℃ to obtain Bacillus subtilis YXSY-01 bacterial powder. The viable number of Bacillus subtilis YXSY-01 in the Bacillus subtilis YXSY-01 bacterial powder was 1.0×10⁻⁶. 10 ~5.0×10 10 Between CFU / g.

[0041] The preparation method of aerobic sphingosomonas HD-01 bacterial suspension is as follows:

[0042] (1) Preparation of fermentation broth of aquatic sphingosomonas HD-01: fermentation broth of aquatic sphingosomonas HD-01 was obtained according to the preparation method of the patent with publication number CN118703394B;

[0043] (2) Preparation of Sphingomonas HD-01 bacterial culture: The fermentation broth of Sphingomonas HD-01 was passed through a 100-mesh sieve to obtain Sphingomonas HD-01 bacterial culture. The viable count of Sphingomonas HD-01 in the Sphingomonas HD-01 bacterial culture was 1.0 × 10⁻⁶. 7 ~2.0×10 8 Between CFU / g.

[0044] The preparation method of biological bottom-modified particles is carried out according to the following steps:

[0045] Step 1: Mix 10g of Bacillus subtilis YXSY-01 bacterial powder and 15g of Sphingomonas aquaticis HD-01 bacterial suspension evenly to obtain a mixture.

[0046] Step 2: Spray the mixture obtained in Step 1 onto 400g of oyster shell powder with a diameter of 2-3mm, and mix evenly.

[0047] Step 3: Add 450g bentonite, 40g zeolite powder, 10g raw fish bone powder and 30g attapulgite to the mixture obtained in step 2, and stir until well mixed.

[0048] Step 4: Add 45g of purified water to the mixture obtained in Step 3, mix thoroughly, and then granulate using a granulator to obtain bio-based bottom-modified granules. These granules are pale yellow in color and contain 1.0 × 10⁻⁶ probiotics. 8 CFU / g - 5.0 × 10 8 CFU / g has a wide range of applications.

[0049] Comparative Example 1

[0050] A type of bio-based bottom-modifying granule has the following formula (i.e., the raw materials required to produce 1 kg of bio-based bottom-modifying granules):

[0051] 10g of commercially available 100 billion Bacillus subtilis (purchased from Dayu Biotechnology) bacterial powder, 15g of commercially available 100 billion Bacillus licheniformis (purchased from Dayu Biotechnology) bacterial powder, 450g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 40g of zeolite powder, 10g of raw fish bone powder, and 45g of purified water.

[0052] The preparation method of biological bottom-modified particles is carried out according to the following steps:

[0053] Step 1: Mix 10g of commercially available 100 billion Bacillus subtilis (purchased from Dayu Biotechnology) bacterial powder and 15g of commercially available 100 billion Bacillus licheniformis (purchased from Dayu Biotechnology) bacterial powder evenly to obtain a mixture.

[0054] Step 2: Spray the mixture obtained in Step 1 onto 400g of oyster shell powder with a diameter of 2-3mm, and mix evenly.

[0055] Step 3: Add 450g bentonite, 40g zeolite powder, 10g raw fish bone powder and 30g attapulgite to the mixture obtained in step 2, and stir until well mixed.

[0056] Step 4: Add 45g of purified water to the mixture obtained in Step 3, mix thoroughly, and then granulate using a granulator to obtain bio-based bottom-modified granules. These granules are pale yellow in color and contain 1.0 × 10⁻⁶ probiotics. 8 CFU / g - 5.0 × 10 8 CFU / g.

[0057] Example 1: Experimental study on the effect of the biological bottom-modifying particles prepared in Example 1 and Comparative Example 1 on the water quality of Litopenaeus vannamei culture.

[0058] The pond information processed is shown in Table 1.

[0059] Table 1. Processed pond information

[0060] The treatment cycle is 2 months. It is applied starting from the 30th day of Litopenaeus vannamei farming, once every 5 days, for a total of 6 applications. The dosage of biological bottom improvement granules is 1 kg / acre each time.

[0061] The fecal content and nitrite content of water samples were determined before and after treatment, and the results are shown in Table 2. The method for detecting fecal content in the water was as follows: Water from the lower layer of the pond (5 cm above the bottom) was taken and filtered with neutral filter paper. Ten times the volume of distilled water was added to the filter residue for rinsing (shaking at 80 rpm for 10 minutes). After rinsing, the residue was filtered again with neutral filter paper, and this rinsing process was repeated three times. The fecal residue was dried in a ventilated drying oven at 55°C until constant weight. The method for detecting nitrite was based on GB / T 2367-2016.

[0062] Table 2. Survey Results After Processing

[0063]

[0064] As shown in Table 2, compared with the bio-bottom-modifying particles prepared in Comparative Example 1, the treatment with the bio-bottom-modifying particles prepared in Example 1 can significantly reduce the nitrite content and fecal content in the water used for Litopenaeus vannamei farming.

[0065] Example 2

[0066] A type of bio-based bottom-modifying granule has the following formula (i.e., the raw materials required to produce 1 kg of bio-based bottom-modifying granules):

[0067] 10g of Bacillus subtilis YXSY-01 bacterial powder, 15g of Sphingomonas aquaticis HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 55g of zeolite powder, 15g of raw fish bone powder, and 45g of purified water.

[0068] Bacillus subtilis YXSY-01 bacterial powder and Sphingomonas aquaticis HD-01 bacterial suspension were prepared in Example 1.

[0069] The preparation method of biological bottom-modified particles is carried out according to the following steps:

[0070] Step 1: Mix 10g of Bacillus subtilis YXSY-01 bacterial powder and 15g of Sphingomonas aquaticis HD-01 bacterial suspension evenly to obtain a mixture.

[0071] Step 2: Spray the mixture obtained in Step 1 onto 440g of oyster shell powder with a diameter of 2-3mm, and mix evenly.

[0072] Step 3: Add 430g bentonite, 40g zeolite powder, 10g raw fish bone powder and 30g attapulgite to the mixture obtained in step 2, and stir until well mixed.

[0073] Step 4: Add 45g of purified water to the mixture obtained in Step 3, mix thoroughly, and then granulate using a granulator to obtain bio-based bottom-modified granules. These granules are pale yellow in color and contain 1.0 × 10⁻⁶ probiotics. 8 CFU / g - 5.0 × 10 8 CFU / g has a wide range of applications.

[0074] Example 2: Experimental study on the effect of the biological bottom-modifying particles prepared in Example 2 on the water quality of California bass aquaculture.

[0075] The information on the fishponds processed is shown in Table 3.

[0076] Table 3. Processed fishpond information

[0077]

[0078] The treatment cycle is 3 months. It is used starting from the 100th day of breeding, once every 5 days, for a total of 18 times. The dosage of biological bottom improvement granules is 1 kg / mu each time.

[0079] The fecal content and nitrite content of water samples were determined before and after treatment, and the results are shown in Table 4. The method for detecting fecal content in the water was as follows: Water from the lower layer of the pond (5 cm above the bottom) was taken and filtered with neutral filter paper. The filtered residue was then rinsed with 10 times its volume of distilled water (shaking at 80 rpm for 10 minutes). After rinsing, the residue was filtered again with neutral filter paper, and this rinsing process was repeated three times. The fecal residue was dried in a ventilated drying oven at 55°C until constant weight. The method for detecting nitrite was based on GB / T 2367-2016.

[0080] Table 4. Survey Results After Processing

[0081]

[0082] As shown in Table 4, the biological bottom-improving particles provided by this invention can significantly reduce the content of nitrite and feces in water.

[0083] Example 3

[0084] Experimental Group 1: 10g of Bacillus subtilis YXSY-01 bacterial powder, 15g of Sphingomonas aquaticis HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 55g of zeolite powder, 15g of raw fish bone powder, and 45g of purified water.

[0085] Experimental Group 2: 25g of Bacillus subtilis YXSY-01 bacterial powder, 0g of aquatic sphingomyelin-monosporium HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 55g of zeolite powder, 15g of raw fish bone powder, and 45g of purified water.

[0086] Experimental Group 3: 0g of Bacillus subtilis YXSY-01 bacterial powder, 25g of aquatic sphingomyelin-monosporium HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 55g of zeolite powder, 15g of raw fish bone powder, and 45g of purified water.

[0087] Experimental Group 4: 20g of Bacillus subtilis YXSY-01 bacterial powder, 5g of Sphingomonas aquaticis HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 30g of attapulgite, 55g of zeolite powder, 15g of raw fish bone powder, and 45g of purified water.

[0088] Experimental Group 5: 10g of Bacillus subtilis YXSY-01 bacterial powder, 15g of aquatic sphingomyelin-monosporium HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 35g of attapulgite, 55g of zeolite powder, 10g of raw fish bone powder, and 45g of purified water.

[0089] Experimental Group 6: 10g of Bacillus subtilis YXSY-01 bacterial powder, 15g of Sphingomonas aquaticis HD-01 bacterial solution, 430g of bentonite, 400g of oyster shell powder with a diameter of 2-3mm, 25g of attapulgite, 55g of zeolite powder, 20g of raw fish bone powder, and 45g of purified water.

[0090] Bacillus subtilis YXSY-01 bacterial powder and Sphingomonas aquaticis HD-01 bacterial suspension were prepared in Example 1.

[0091] Granulation was performed according to the granulation method of Example 2.

[0092] Bottom water samples (5cm above the bottom of the pond) from different California bass ponds were collected and dispensed into 1000mL beakers, with 800mL of liquid in each beaker. After standing for 30 minutes, 1g of bottom conditioner granules was added to each group, and the samples were left to stand for 72 hours. The nitrite content in the water was then measured, with three replicates per group.

[0093] The information on the fishponds processed is shown in Table 5.

[0094] Table 5. Processed fishpond information

[0095]

[0096] The treatment cycle is 3 months. It is used starting from the 100th day of breeding, once every 5 days, for a total of 18 times. The dosage of biological bottom improvement granules is 1 kg / mu each time.

[0097] The fecal content and nitrite content of water samples were determined before and after treatment, and the results are shown in Table 6. The method for detecting fecal content in the water was as follows: filtration with neutral filter paper, adding 10 times the volume of distilled water to the filtered residue for rinsing (shaking at 80 rpm for 10 min), followed by filtration with neutral filter paper, and repeating the rinsing process three times. The fecal residue was dried in a ventilated drying oven at 55℃ until constant weight. The method for detecting nitrite was based on GB / T 2367-2016.

[0098] Table 6. Survey Results After Processing

[0099]

[0100] As shown in Table 6, experimental group 1 had the best overall effect, followed by experimental group 4 with varying amounts of the two strains. Then came experimental groups 2 and 3, where one strain was omitted, and experimental groups 5 and 6, where the amount of excipients was varied. This demonstrates that the amount and combination of the two strains, as well as the amount of excipients, all affect the treatment effect. However, within the scope of this invention, the effect is optimal.

[0101] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A biological bottom modification composition, characterized in that, The components include the following parts by weight: 0.3-1.7 parts Bacillus subtilis YXSY-01 bacterial powder, 0.2-2 parts Sphingomonas aquatilis HD-01 bacterial solution, 30-50 parts bentonite, 30-40 parts oyster shell powder, 1-3 parts attapulgite, 2-6 parts zeolite powder, 1-1.5 parts raw fish bone meal, and 1.5-4.5 parts water; The preservation number of Bacillus subtilis YXSY-01 is GDMCC No: 64078; the preservation number of Sphingomonas aquaticus HD-01 is GDMCC No: 64330.

2. The biological bottom modification composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: 1 part Bacillus subtilis YXSY-01 bacterial powder, 1.5 parts aquatic sphingomonas HD-01 bacterial solution, 45 parts bentonite, 40 parts oyster shell powder, 3 parts attapulgite, 4 parts zeolite powder, 1 part raw fish bone powder and 4.5 parts water. Alternatively, 1 part Bacillus subtilis YXSY-01 bacterial powder, 1.5 parts aquatic sphingomonas HD-01 bacterial solution, 43 parts bentonite, 40 parts oyster shell powder, 3 parts attapulgite, 5.5 parts zeolite powder, 1.5 parts raw fish bone powder, and 4.5 parts water.

3. The biological bottom-modifying composition according to claim 1, characterized in that, The viable count of Bacillus subtilis YXSY-01 in the Bacillus subtilis YXSY-01 powder was 1.0 × 10⁻⁶. 10 ~5.0×10 10 CFU / g; The viable count of *Sphingomonas aquaticus* HD-01 in the bacterial culture was 1.0 × 10⁻⁶. 7 ~2.0×10 8 CFU / g; The oyster shell powder is oyster shell powder with a diameter of 2~3mm.

4. The use of the bio-bottom modification composition according to any one of claims 1 to 3 in the preparation of bio-bottom modification formulations.

5. A biological bottom-modifying agent, characterized in that, Includes the biomodified composition according to any one of claims 1 to 3.

6. The biological bottom-modifying agent according to claim 5, characterized in that, The biological bottom-modifying agent is in granule form.

7. The method for preparing the biological bottom-modifying agent according to claim 5, characterized in that, Includes the following steps: The mixture of Bacillus subtilis YXSY-01 bacterial powder and Sphingomonas aquaticis HD-01 bacterial solution is mixed with the oyster shell powder to obtain a mixture; The mixture is combined with the bentonite, the zeolite powder, the raw fish bone powder, the attapulgite clay, and the water to obtain the biological bottom modifier.

8. The use of the biological bottom-modifying composition according to any one of claims 1 to 3 or the biological bottom-modifying agent according to claim 5 or 6 in the degradation of feces and / or reduction of nitrite content in water bodies.

9. A method for degrading feces and / or reducing the nitrite content in water, characterized in that, The step includes applying the biological bottom modifier as described in claim 5 or 6 into the water body.

10. The method according to claim 9, characterized in that, The application rate of the biological bottom improvement agent is 1 kg / time / acre; the application interval is 5 days.

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