Microfluidic single cell culture plate and method based on AI image recognition micropore positioning technology

By using AI image recognition technology and microfluidic single-cell culture plates, the problems of inaccurate microwell positioning and resource occupation of the microscopic system in existing technologies have been solved, realizing efficient single-cell culture and drug screening, and improving drug development efficiency and data analysis accuracy.

CN120924402APending Publication Date: 2025-11-11CYROWAN TEST CO LTD
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Patent Information

Application Number
CN202511041403.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-28
Publication Date
2025-11-11

AI Technical Summary

Technical Problem

Existing technologies are insufficient for high-throughput single-cell culture and drug screening. Furthermore, existing microwell positioning methods are easily affected by environmental factors, leading to inaccurate positioning and an inability to effectively resolve single-cell-level drug efficacy heterogeneity. Additionally, long-term observation of a single cell requires the use of microscopic system resources.

Method used

This microfluidic single-cell culture plate utilizes AI image recognition for microwell localization technology. Combining microfluidics and artificial intelligence image recognition, it achieves rapid and accurate microwell localization through microwell pattern arrangement. It supports cell location identification and drug screening at multiple time points, is compatible with mainstream microscopy systems, and enables global microwell localization and automated data analysis.

Benefits of technology

It enables high-throughput single-cell culture and drug screening, improves accuracy, reduces the time spent on the microscopy system, supports multi-timepoint drug comparison experiments, improves the accuracy and automation of data analysis, and reduces drug development costs.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to a microfluidic single cell culture plate based on AI image recognition and a global positioning method. A micropore array is arranged in the culture plate, a unique code is formed according to an asymmetric micropore pattern in a micropore positioning area, a global positioning method identifies the positioning pattern through a microscope bright field image, rapid and accurate positioning of micropores is realized in combination with an AI algorithm, dynamic tracking of cell positions in multi-time-point sampling is supported, and the positioning accuracy is improved. Drug testing of different drug use sequences is achieved, the problems of low micropore positioning efficiency and long-term occupation under a traditional microscope are solved, and cell-level drug effect data are provided for drug screening (targeted therapy / drug combination) and personalized medical treatment.
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Citation Information

Patent Citations

  • A microfluidic chip for single-cell capture and culture

    CN106497786B

  • Bioanalysis chip capable of realizing cell capture and fixation

    CN111774110A