Anti-aging primordial qi-consolidating and life-prolonging pill and preparation method thereof

Abstract

The invention discloses an anti-aging primordial qi-consolidating and life-prolonging pill and a preparation method thereof. The preparation method of the anti-aging primordial qi strengthening and life prolonging pill comprises the following steps: heating radix ophiopogonis, radix asparagi, radix rehmanniae, radix rehmanniae preparata, Chinese yam, rhizoma alismatis, poria cocos, radix achyranthis bidentatae, fructus corni, eucommia ulmoides, radix salviae miltiorrhizae, fructus lycii, raspberry, cortex lycii radicis, swimming bladder beads and pearls by adopting a solvent and an auxiliary agent; heating ginseng, polygala tenuifolia, platycladi seed, morinda officinalis (salted), cistanche, wolfberry, deer-horn glue, semen cuscutae and dog penis by adopting an enzyme preparation, an enzyme protective agent and a regulatory factor; performing extraction treatment on schisandra chinensis, radix aucklandiae, pepper, rhizoma acori graminei and fructus lycii by adopting supercritical CO2; finally, respectively sterilizing the extract and the filter residues, and uniformly mixing to obtain a mixed material; and mixing the mixed material with auxiliary materials, refining honey and pelleting to obtain the anti-aging primordial qi-consolidating and life-prolonging pill. Differentiated extraction is carried out on hydrophilic, enzymolysis sensitive and fat-soluble components in a compound respectively, and conflicts of extraction conditions among the components are avoided.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, and in particular to an anti-aging tonic pill and its preparation method. Background Technology

[0002] Gubenyanling Pills are a nationally approved medicine. The main ingredients include ginseng, ophiopogon japonicus, schisandra chinensis, asparagus cochinchinensis, rehmannia glutinosa, prepared rehmannia glutinosa, yam, alisma plantago-aquatica, poria cocos, achyranthes bidentata, cornus officinalis, eucommia ulmoides, costus root, polygala tenuifolia, arborvitae seed, morinda officinalis (salt-processed), zanthoxylum bungeanum, acorus tatarinowii, salvia miltiorrhiza, cistanche deserticola, wolfberry, raspberry, lycium chinense root bark, deer antler glue, cuscuta chinensis, fish maw pearl, pearl, and dog penis. The excipient is honey. After drying, pulverizing, sterilizing, and mixing the above medicinal materials, refined honey is added to make large honey pills. Functions and indications: Strengthens the body's foundation, nourishes yin, replenishes essence, and strengthens bones and muscles. Used for weakness and injury, lower back pain and fatigue, palpitations and insomnia, haggard skin, premature graying of hair, irregular menstruation, and loss of appetite.

[0003] The existing technology for making Gubenyanling pills involves grinding the raw materials of traditional Chinese medicine into powder and then directly forming them into pills. However, this method easily retains unpleasant sensory factors such as coarse fiber, bitterness, and astringency. When raw Chinese medicine powder is directly used in medicine, the release of active ingredients depends on gastrointestinal digestion and absorption, which can lead to insufficient absorption and slow onset of action. Furthermore, some raw materials of traditional Chinese medicine are rich in viscous polysaccharides; directly grinding them and then adding them to pills may result in pills that are not properly formed or that absorb excessive moisture.

[0004] CN119632935A discloses an anti-aging tonic pill and its preparation method. The method involves pulverizing raw materials using dual-frequency ultrasonic extraction combined with solvent extraction to obtain highly active, high-antioxidant components. The extract and pulverized filter residue are then mixed in a specific ratio through microfiltration, sterilized, and mixed with modified microcrystalline cellulose and other excipients. The mixture is then refined with honey and formed into pills, resulting in a highly stable, high-quality, and highly antioxidant tonic pill. However, this method suffers from significant loss of active ingredients. Summary of the Invention

[0005] In view of the above-mentioned deficiencies of the prior art, the technical problem to be solved by the present invention is to provide a traditional Chinese medicine for strengthening the body and prolonging life with anti-aging function.

[0006] Some components of traditional Chinese medicine, such as fat-soluble flavonoids, sterols, small protein peptides, and trace elements, are difficult to fully dissolve during water extraction. Directly using the extract to make pills will result in the loss of these substances. However, remixing the extract with the residue can retain trace active components in the residue, achieving full utilization of the components and synergistic enhancement of efficacy. The concentrated paste after water extraction is generally viscous and lacks compressibility and formability. The residue, as a natural adsorbent carrier and structural framework, can significantly improve the fluidity, mechanical strength, and drying properties of pills, helping to form pills with intact shape and strong stability. Using concentrated extracts alone to make pills usually requires the addition of large amounts of enzyme protectants such as honey, syrup, and dextrin, which may affect the dissolution rate or the taste of the finished product. The residue can act as a physical filler and binder, reducing the proportion of added excipients. At the same time, some residues contain natural cellulose, hemicellulose, and polysaccharides, which have a certain water swelling capacity. When mixed with concentrated extracts to form pills, they can form a slow disintegration and segmented release system, which helps to prolong the duration of efficacy and improve intestinal absorption.

[0007] Existing technologies lack precision in extraction processes. Traditional decoction methods have low extraction efficiency for volatile and fat-soluble components. When multiple Chinese herbal raw materials are mixed for extraction, there are issues such as component degradation, incompatibility, or encapsulation and inactivation.

[0008] To achieve the above objectives, the present invention provides an anti-aging and longevity-enhancing pill, characterized by comprising: ginseng, ophiopogon japonicus, schisandra chinensis, asparagus cochinchinensis, rehmannia glutinosa, prepared rehmannia glutinosa, yam, alisma plantago-aquatica, poria cocos, achyranthes bidentata, cornus officinalis, eucommia ulmoides, costus root, polygala tenuifolia, arborvitae seed, morinda officinalis (salt-processed), zanthoxylum bungeanum, acorus tatarinowii, salvia miltiorrhiza, cistanche deserticola, wolfberry, raspberry, lycium chinense root bark, deer antler glue, cuscuta chinensis, fish maw pearl, pearl, and dog penis.

[0009] A further preferred anti-aging tonic pill includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0010] This invention also discloses a method for preparing an anti-aging tonic pill;

[0011] A method for preparing an anti-aging and longevity-enhancing pill includes the following steps:

[0012] (1) Weigh out the Chinese medicinal materials according to the formula;

[0013] (2) The following herbs were dried and pulverized: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum, Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. The above-mentioned Chinese herbal powders were then mixed evenly. The above-mentioned Chinese herbal powders were mixed evenly with water and auxiliary agents, and the pH was adjusted by phosphate buffer solution. The mixture was then heated to obtain extract 1 and filter residue 1.

[0014] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum, deer antler glue, Cuscuta chinensis and dog penis are dried and pulverized respectively. Then the above Chinese medicine powders are mixed evenly. The above Chinese medicine powders are added to water, and then enzyme preparation, enzyme protectant and regulatory factor are added. The mixture is heated and the enzyme is inactivated after the treatment. The mixture is filtered to obtain extract 2 and residue 2.

[0015] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum respectively, then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3;

[0016] (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0017] (6) Mix the above mixture with the auxiliary materials, refine the honey and make pills to obtain anti-aging and longevity pills.

[0018] Furthermore, a method for preparing an anti-aging and longevity-enhancing pill includes the following steps:

[0019] (1) Weigh out the Chinese medicinal materials according to the formula;

[0020] (2) Dry and pulverize the following herbs: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum, Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. Pass them through an 80-120 mesh sieve. Then mix the above-mentioned Chinese herbal powders evenly. Mix the above-mentioned Chinese herbal powders evenly with water and auxiliary agents. Adjust the pH with phosphate buffer solution and heat to 70-90℃ for 1-3 hours. After the treatment, filter to obtain extract 1 and filter residue 1. Evaporate extract 1 by rotary evaporation and dry to obtain extract 1. Dry filter residue 1.

[0021] The ratio of the Chinese herbal powder to water is 1g:(10-20)mL; the adjuvant is mannitol.

[0022] The mass ratio of water to auxiliary agent is (5-10):1;

[0023] The phosphate buffer solution is used to adjust the pH to 6.8-7.2;

[0024] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum, deer antler glue, Cuscuta chinensis, and dog penis are dried and pulverized separately, and passed through an 80-120 mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation, enzyme protectant, and regulatory factor are added. The mixture is heated to 40-50℃ and reacted for 1-3 hours. After the reaction is completed, the enzyme is inactivated, and the mixture is filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.10-1.3 to obtain extract 2. Residue 2 is dried.

[0025] The ratio of Chinese herbal powder to water is 1g:(10-20)mL; the amount of enzyme preparation added is 1.5-3%w / v; wherein the enzyme preparation is at least one of cellulase, protease, and pectinase.

[0026] The enzyme protectant is at least one of mannitol, sorbitol, and trehalose; the amount of enzyme protectant added is 20-80 wt% of the enzyme preparation mass; more preferably 40-60 wt%, used to improve the enzyme's activity retention rate and environmental adaptability during the reaction process.

[0027] Furthermore, the regulatory factor is at least one of quercetin-7-O-glucoside and ectoine; the amount of the regulatory factor added is 1.0-4.0 wt% of the enzyme preparation mass, more preferably 1.5-3.0 wt%; more preferably, the regulatory factor is a mixture of quercetin-7-O-glucoside and ectoine in a mass ratio of 1:(10-20).

[0028] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum respectively, pass them through an 80-120 mesh sieve, and then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; evaporate extract 3 by rotary evaporation and dry to obtain extract 3; dry residue 3.

[0029] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200-220 bar, extraction temperature of 40-45℃, extraction time of 60-120 min, and CO2 flow rate of 1-6 mL / min. The entrainer for supercritical CO2 extraction was ethanol, with a flow rate of 1-2 mL / min. The flow ratio of entrainer to CO2 was 1:(3-6).

[0030] (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0031] (6) Mix the above mixture with the auxiliary materials, refine the honey and make pills to obtain anti-aging and longevity pills.

[0032] The primary purpose of adding phosphate buffer solution during the water extraction of traditional Chinese medicine (TCM) is to maintain the pH stability of the extraction environment. TCM raw materials often contain heat-sensitive components such as esters, glycosides, polysaccharides, and proteins. These components are extremely sensitive to pH during extraction; fluctuations in the system's pH can easily lead to adverse reactions such as hydrolysis, oxidation, and polymerization. Adjusting the pH effectively suppresses significant pH fluctuations caused by the release of organic acids or alkaline substances during extraction, protecting the structural integrity of active ingredients and improving their stability. Furthermore, the buffer solution can also help improve the dissolution efficiency of some target components such as flavonoids and saponins. Mannitol, a natural polyol with good water solubility and biocompatibility, can be added during water extraction to form multi-point hydrogen bonds between its hydroxyl groups and polar groups such as proteins, polysaccharides, and flavonoids, thereby forming a protective layer on the outside of the molecule. This effectively inhibits aggregation and precipitation, enhancing the structural stability of the active components.

[0033] Some traditional Chinese medicine raw materials are treated with enzyme preparations. Introducing enzyme protectants during this process is beneficial for increasing the content of active ingredients and protecting easily degradable substances during extraction. Enzyme protectants, as natural polyols rich in multiple hydroxyl groups, can form hydrogen bonds with amino acid residues on the enzyme surface, reducing enzyme activity loss caused by temperature fluctuations or pH changes. Furthermore, enzyme protectants have a certain osmotic regulation ability, which can alleviate the inhibition of enzyme activity by substrate concentration, improving enzyme adaptability and reaction efficiency. In addition, enzyme protectants can increase the solution viscosity and buffering capacity of the system, preventing non-selective or excessive hydrolysis of active ingredients due to excessively rapid reactions. The regulatory factors quercetin-7-O-glucoside and ectoine exhibit a significant synergistic effect. As a regulatory factor, quercetin-7-O-glucoside can form hydrogen bonds with the enzyme's active site through its glycosidic structure. Simultaneously, its aromatic ring structure inserts into the hydrophobic region of the enzyme, constructing a non-active, stable complex that effectively blocks the entry of non-substrate and the non-selective degradation of key substrates. Ectoyline, by forming a stable hydrated shell microenvironment, protects the enzyme protein conformation and enhances its resistance to adverse factors such as temperature fluctuations and oxidative stress. The combined application of these two agents helps stabilize the enzyme's spatial structure, prolonging its action time, and enhances the specific protection of the target substrate, thereby improving the overall efficiency and precision of the reaction system.

[0034] The beneficial effects of this invention are:

[0035] 1. Compared with existing technologies, introducing phosphate buffer during water extraction effectively maintains the acid-base stability of the extraction environment, preventing degradation reactions such as hydrolysis, polymerization, and precipitation of heat-sensitive components like saponins, flavonoids, polysaccharides, and proteins due to pH drift, thereby improving the extraction efficiency and stability of the target components. The addition of mannitol further forms a protective layer through hydrogen bonding, effectively inhibiting thermal denaturation and adverse antagonistic reactions between components, enhancing the structural integrity and dissolution effect of the components during extraction.

[0036] 2. Compared with existing technologies, this method uses water extraction, enzymatic hydrolysis, and supercritical CO2 extraction of Chinese medicine to extract hydrophilic, enzymatically sensitive, and lipid-soluble components in the compound, thus avoiding conflicts in extraction conditions between components.

[0037] 3. The extracts are concentrated and dried under reduced pressure. The filter residues from each stage are dried and then mixed and reused, resulting in a high comprehensive utilization rate of materials. The moisture content of the final product is controlled to be no more than 6% and meets the microbial limit requirements, ensuring the safety and stability of Gubenyanling Pills during long-term storage. Attached Figure Description

[0038] Figure 1 This is a significant analysis chart of the general appearance of rats in each group (Note: ## p < 0.01, compared with the normal group; * p < 0.05 (compared with the model group).

[0039] Figure 2 This is a graph showing the significance of tail suspension resting time in each group of rats (Note: ## p < 0.01, compared with the normal group; ** p < 0.01 (compared with the model group).

[0040] Figure 3 This is a graph showing the significance of swimming time in each group of rats (Note: ## p < 0.01, compared with the normal group; ** p < 0.01 (compared with the model group).

[0041] Figure 4 This is a comparison of HE staining of kidney tissue from the normal group, model group, Jin Kui Shen Qi Wan group, and vitamin E group.

[0042] Figure 5 This is a comparison of HE staining of kidney tissue from the high-dose group, medium-dose group, and low-dose group of Gubenyanling Pills. Detailed Implementation

[0043] The endpoints and any values ​​of the ranges disclosed in this invention are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of the various ranges, the endpoint values ​​of the various ranges and individual point values, and individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this invention.

[0044] Description of some of the raw materials used in the embodiments of this invention:

[0045] Rehmannia glutinosa, manufactured by Jiangxi Jiangzhong Chinese Herbal Medicine Pieces Co., Ltd.; production batch number 2411043;

[0046] Rehmannia glutinosa (processed), manufactured by Jiangxi Jiangzhong Chinese Herbal Medicine Pieces Co., Ltd.; production batch number 241119;

[0047] Morinda officinalis (salt-processed), produced by Jiangxi Jiangzhong Chinese Herbal Medicine Pieces Co., Ltd.; production batch number 240826;

[0048] Cellulase, product number: S10041, purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0049] The protease was papain, product number: S27241, purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0050] Pectinase, product number: R096759, purchased from Shanghai E. En Chemical Technology Co., Ltd.

[0051] The specific method for preparing refined honey used in the embodiment is as follows: take raw honey, add water and boil it to melt it, then pass it through a 40-mesh sieve, continue heating to a temperature of 115°C, water content of 14%, relative density of 1.38, and fine bubbles that are uniformly bubbling appear.

[0052] Other raw materials not mentioned are all common raw materials. The above content is only for the purpose of illustrating the present invention and should not be construed as a strict limitation of the present invention. Those skilled in the art can directly purchase the same / similar raw materials from the market or prepare them themselves.

[0053] The technical solutions of the present invention will be clearly and completely described below with reference to specific embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0054] Example 1

[0055] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0056] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0057] (1) Weigh out the Chinese medicinal materials according to the formula;

[0058] (2) Ginseng, Ophiopogon japonicus, Schisandra chinensis, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Aucklandia lappa, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Zanthoxylum bungeanum, Acorus tatarinowii, Salvia miltiorrhiza, Cistanche deserticola, Lycium barbarum, Rubus idaeus, Lycium chinense root bark, deer antler glue, Cuscuta chinensis, fish maw pearl, pearl, and dog penis were dried, pulverized, and passed through an 80-mesh sieve. The above Chinese herbal powders were then mixed evenly. The above Chinese herbal powders were mixed evenly with water and mannitol, and the pH was adjusted to 7.2 using phosphate buffer. The mixture was heated to 75°C for 2 hours. After the treatment, the mixture was filtered to obtain the extract and the residue. The extract was evaporated by rotary evaporation and dried to obtain the extract. The residue was dried.

[0059] The ratio of Chinese medicine powder to water is 1g:15mL;

[0060] The mass ratio of water to mannitol is 9:1.

[0061] (3) After sterilizing the above extract and filter residue separately, mix them evenly to obtain a mixture;

[0062] (4) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills. The excipient is refined honey, the temperature of which is 60°C, and 110g of refined honey is added for every 100g of mixture.

[0063] Example 2

[0064] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0065] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0066] (1) Weigh out the Chinese medicinal materials according to the formula;

[0067] (2) Ginseng, Ophiopogon japonicus, Schisandra chinensis, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Aucklandia lappa, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Zanthoxylum bungeanum, Acorus tatarinowii, Salvia miltiorrhiza, Cistanche deserticola, Lycium barbarum, Rubus idaeus, Lycium chinense root bark, deer antler glue, Cuscuta chinensis, fish maw pearl, pearl, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation, enzyme protectant, and regulatory factor are added. The mixture is heated to 50°C and reacted for 3 hours. After the reaction, the enzyme is inactivated, and the mixture is filtered to obtain the extract and residue. The extract is concentrated to a relative density of 1.15 to obtain the extract. The residue is dried.

[0068] The ratio of Chinese herbal powder to water is 1g:15mL; the amount of enzyme preparation added is 2%w / v.

[0069] The enzyme preparation mentioned above is composed of cellulase, protease, and pectinase mixed in a mass ratio of 1:1:1.

[0070] The enzyme protectant is trehalose; the amount of enzyme protectant added is 50 wt% of the enzyme preparation mass; the amount of regulatory factor added is 2.0 wt% of the enzyme preparation mass; the regulatory factor is a mixture of quercetin-7-O-glucoside and ectoine at a mass ratio of 1:10.

[0071] (3) After sterilizing the above extract and filter residue separately, mix them evenly to obtain a mixture;

[0072] (4) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0073] Example 3

[0074] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0075] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0076] (1) Weigh out the Chinese medicinal materials according to the formula;

[0077] (2) Ginseng, Ophiopogon japonicus, Schisandra chinensis, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Aucklandia lappa, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Zanthoxylum bungeanum, Acorus tatarinowii, Salvia miltiorrhiza, Cistanche deserticola, Lycium barbarum, Rubus idaeus, Lycium chinense root bark, deer antler glue, Cuscuta chinensis, fish maw pearl, pearl, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the powders of the above Chinese herbs are mixed evenly. Then, supercritical CO2 extraction is performed. After the extraction is completed, the extract and residue are filtered. The extract is evaporated by rotary evaporation and dried to obtain the extract. The residue is dried.

[0078] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0079] (3) After sterilizing the above extract and filter residue separately, mix them evenly to obtain a mixture;

[0080] (4) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0081] Example 4

[0082] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0083] The preparation method of anti-aging and longevity-enhancing pills includes the following steps:

[0084] (1) Weigh out the Chinese medicinal materials according to the formula;

[0085] (2) The following herbs were dried and pulverized separately: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (30g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, pearl, Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, and Acorus tatarinowii. They were then passed through an 80-mesh sieve. The above-mentioned herbal powders were then mixed evenly. The above-mentioned herbal powders were mixed evenly with water and mannitol. The pH was adjusted to 7.2 using phosphate buffer solution. The mixture was heated to 75°C for 3 hours. After the treatment, the mixture was filtered to obtain extract 1 and residue 1. Extract 1 was evaporated by rotary evaporation and dried to obtain extract 1. Residue 1 was then dried.

[0086] The ratio of Chinese medicine powder to water is 1g:15mL;

[0087] The mass ratio of water to mannitol is 9:1.

[0088] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum (15g), deer antler glue, Cuscuta chinensis, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation and enzyme protectant are added. The mixture is heated to 50°C and reacted for 3 hours. After the reaction is completed, the enzyme is inactivated and filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.15 to obtain extract 2. Residue 2 is dried.

[0089] The ratio of Chinese herbal powder to water is 1g:15mL; the amount of enzyme preparation added is 2%w / v.

[0090] The enzyme preparation is composed of cellulase, protease, and pectinase mixed in a mass ratio of 1:1:1; the enzyme protectant is trehalose; and the amount of enzyme protectant added is 50 wt% of the enzyme preparation.

[0091] (4) After sterilizing the above extract 1, extract 2, filter residue 1, and filter residue 2 respectively, mix them evenly to obtain a mixture.

[0092] (5) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0093] Example 5

[0094] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0095] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0096] (1) Weigh out the Chinese medicinal materials according to the formula;

[0097] (2) The following herbs were dried and pulverized: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (15g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. They were then passed through an 80-mesh sieve. The above-mentioned herbal powders were then mixed evenly. The above-mentioned herbal powders were mixed evenly with water and mannitol. The pH was adjusted to 7.2 using phosphate buffer, and the mixture was heated to 75°C for 3 hours. After the treatment, the mixture was filtered to obtain extract 1 and residue 1. Extract 1 was evaporated by rotary evaporation and dried to obtain extract 1. Residue 1 was then dried.

[0098] The ratio of Chinese medicine powder to water is 1g:15mL;

[0099] The mass ratio of water to mannitol is 9:1.

[0100] (3) The following herbs were dried, pulverized, and passed through an 80-mesh sieve: Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii, Lycium barbarum (30g), Panax ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, deer antler glue, Cuscuta chinensis, and Cibotium barometz. The powders were then mixed evenly and subjected to supercritical CO2 extraction. After extraction, the extract was filtered to obtain extract 3 and residue 3. Extract 3 was evaporated by rotary evaporation and dried to obtain extract 3. Residue 3 was dried.

[0101] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0102] (4) After sterilizing the above extract 1, extract 3, filter residue 1 and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0103] (5) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0104] Example 6

[0105] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0106] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0107] (1) Weigh out the Chinese medicinal materials according to the formula;

[0108] (2) The following herbs were dried and pulverized separately: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (15g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. The powders were then mixed evenly. The powders were mixed evenly with water and mannitol. The pH was adjusted to 7.2 using phosphate buffer. The mixture was heated to 75°C for 2 hours. After the treatment, the mixture was filtered to obtain extract 1 and residue 1. Extract 1 was evaporated by rotary evaporation and dried to obtain extract 1. Residue 1 was dried.

[0109] The ratio of Chinese medicine powder to water is 1g:15mL;

[0110] The mass ratio of the aqueous solution to mannitol is 9:1.

[0111] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum (15g), deer antler glue, Cuscuta chinensis, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation, enzyme protectant, and regulatory factor are added. The mixture is heated to 50°C and reacted for 3 hours. After the reaction is completed, the enzyme is inactivated, and the mixture is filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.15 to obtain extract 2. Residue 2 is dried.

[0112] The ratio of Chinese herbal powder to water is 1g:15mL; the amount of enzyme preparation added is 2%w / v.

[0113] The enzyme preparation is composed of cellulase, protease, and pectinase mixed in a mass ratio of 1:1:1; the enzyme protectant is trehalose; the amount of enzyme protectant added is 50 wt% of the enzyme preparation; the amount of regulatory factor added is 2.0 wt% of the enzyme preparation; the regulatory factor is composed of quercetin-7-O-glucoside and ectoine mixed in a mass ratio of 1:10.

[0114] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum (15g) separately, pass them through an 80-mesh sieve, and then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; evaporate extract 3 by rotary evaporation and dry to obtain extract 3; dry residue 3.

[0115] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0116] (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0117] (6) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0118] Example 7

[0119] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0120] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0121] (1) Weigh out the Chinese medicinal materials according to the formula;

[0122] (2) Dry and pulverize the following herbs: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (15g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. Then, mix the above-mentioned Chinese herbal powders evenly. Mix the above-mentioned Chinese herbal powders evenly with water and heat to 75°C for 2 hours. After the treatment, filter to obtain extract 1 and residue 1. Evaporate extract 1 by rotary evaporation and dry to obtain extract 1. Dry residue 1.

[0123] The ratio of Chinese medicine powder to water is 1g:15mL;

[0124] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum (15g), deer antler glue, Cuscuta chinensis, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation and enzyme protectant are added. The mixture is heated to 50°C and reacted for 3 hours. After the reaction is completed, the enzyme is inactivated and filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.15 to obtain extract 2. Residue 2 is dried.

[0125] The ratio of Chinese medicine powder to water is 1g:15mL;

[0126] The enzyme preparation is added at a rate of 2% w / v; wherein the enzyme preparation is a mixture of cellulase, protease and pectinase in a mass ratio of 1:1:1.

[0127] The enzyme protectant is trehalose; the amount of enzyme protectant added is 50 wt% of the enzyme preparation mass.

[0128] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum (15g) separately, pass them through an 80-mesh sieve, and then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; evaporate extract 3 by rotary evaporation and dry to obtain extract 3; dry residue 3.

[0129] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0130] (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0131] (6) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0132] Example 8

[0133] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0134] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0135] (1) Weigh out the Chinese medicinal materials according to the formula;

[0136] (2) The following herbs were dried and pulverized separately: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (15g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. The powders were then mixed evenly. The powders were mixed evenly with water and mannitol. The pH was adjusted to 7.2 using phosphate buffer. The mixture was heated to 75°C for 2 hours. After the treatment, the mixture was filtered to obtain extract 1 and residue 1. Extract 1 was evaporated by rotary evaporation and dried to obtain extract 1. Residue 1 was dried.

[0137] The ratio of Chinese medicine powder to water is 1g:15mL;

[0138] The mass ratio of water to mannitol is 9:1.

[0139] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum (15g), deer antler glue, Cuscuta chinensis, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water and heated to 50°C for 3 hours. After the reaction is completed, the mixture is filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.15 to obtain extract 2. Residue 2 is dried.

[0140] The ratio of Chinese medicine powder to water is 1g:15mL;

[0141] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum (15g) separately, pass them through an 80-mesh sieve, and then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; evaporate extract 3 by rotary evaporation and dry to obtain extract 3; dry residue 3.

[0142] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0143] (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0144] (6) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0145] Example 9

[0146] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0147] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0148] (1) Weigh out the Chinese medicinal materials according to the formula;

[0149] (2) The following herbs were dried and pulverized separately: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (15g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. The powders were then mixed evenly. The powders were mixed evenly with water and mannitol. The pH was adjusted to 7.2 using phosphate buffer. The mixture was heated to 75°C for 2 hours. After the treatment, the mixture was filtered to obtain extract 1 and residue 1. Extract 1 was evaporated by rotary evaporation and dried to obtain extract 1. Residue 1 was dried.

[0150] The ratio of Chinese medicine powder to water is 1g:15mL;

[0151] The mass ratio of water to mannitol is 9:1.

[0152] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum (15g), deer antler glue, Cuscuta chinensis, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation, enzyme protectant, and regulatory factor are added. The mixture is heated to 50°C and reacted for 3 hours. After the reaction is completed, the enzyme is inactivated, and the mixture is filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.15 to obtain extract 2. Residue 2 is dried.

[0153] The ratio of Chinese herbal powder to water is 1g:15mL; the amount of enzyme preparation added is 2%w / v.

[0154] The enzyme preparation mentioned above is composed of cellulase, protease, and pectinase mixed in a mass ratio of 1:1:1.

[0155] The enzyme protectant is trehalose; the amount of enzyme protectant added is 50 wt% of the enzyme preparation mass; the amount of regulatory factor added is 2.0 wt% of the enzyme preparation mass; and the regulatory factor is ectoine.

[0156] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum (15g) separately, pass them through an 80-mesh sieve, and then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; evaporate extract 3 by rotary evaporation and dry to obtain extract 3; dry residue 3.

[0157] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0158] (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture.

[0159] (6) Mix the above mixture with the excipients, refine the honey and make pills to obtain anti-aging and longevity pills; the excipient is refined honey, the temperature of the refined honey is 60℃, and 110g of refined honey is added for every 100g of mixture.

[0160] Example 10

[0161] An anti-aging tonic pill for strengthening the body and prolonging life includes the following ingredients: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis;

[0162] The preparation method of the anti-aging and longevity-enhancing pill includes the following steps:

[0163] (1) Weigh out the Chinese medicinal materials according to the formula;

[0164] (2) The following herbs were dried and pulverized separately: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum (15g), Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. The powders were then mixed evenly. The powders were mixed evenly with water and mannitol. The pH was adjusted to 7.2 using phosphate buffer. The mixture was heated to 75°C for 2 hours. After the treatment, the mixture was filtered to obtain extract 1 and residue 1. Extract 1 was evaporated by rotary evaporation and dried to obtain extract 1. Residue 1 was dried.

[0165] The ratio of Chinese medicine powder to water is 1g:15mL;

[0166] The mass ratio of water to mannitol is 9:1.

[0167] (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum (15g), deer antler glue, Cuscuta chinensis, and dog penis are dried, pulverized, and passed through an 80-mesh sieve. Then, the above Chinese herbal powders are mixed evenly. The above Chinese herbal powders are added to water, and then enzyme preparation, enzyme protectant, and regulatory factor are added. The mixture is heated to 50°C and reacted for 3 hours. After the reaction is completed, the enzyme is inactivated, and the mixture is filtered to obtain extract 2 and residue 2. Extract 2 is concentrated to a relative density of 1.15 to obtain extract 2. Residue 2 is dried.

[0168] The ratio of Chinese herbal powder to water is 1g:15mL; the amount of enzyme preparation added is 2%w / v.

[0169] The enzyme preparation mentioned above is composed of cellulase, protease, and pectinase mixed in a mass ratio of 1:1:1.

[0170] The enzyme protectant is trehalose; the amount of enzyme protectant added is 50 wt% of the enzyme preparation mass; the amount of regulatory factor added is 2.0 wt% of the enzyme preparation mass; and the regulatory factor is quercetin-7-O-glucoside.

[0171] (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum (15g) separately, pass them through an 80-mesh sieve, and then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; evaporate extract 3 by rotary evaporation and dry to obtain extract 3; dry residue 3.

[0172] The conditions for supercritical CO2 extraction were as follows: extraction pressure of 200 bar, extraction temperature of 45℃, extraction time of 100 min, and CO2 flow rate of 5 mL / min. The entrainer used in the supercritical CO2 extraction was ethanol, with a flow rate of 1.5 mL / min. The flow ratio of entrainer to CO2 was 1:6.

[0173] (5) Sterilize the above-mentioned Extract 1, Extract 2, Extract 3, Filter Residue 1, Filter Residue 2, and Filter Residue 3 respectively, and then mix them evenly to obtain a mixed material;

[0174] (6) Mix the above-mentioned mixed material with auxiliary materials, refine honey and make pills to obtain the Guben Yanling Pills for anti-aging; the auxiliary material is refined honey, the temperature of the refined honey is 60°C, and 110 g of refined honey is added to every 100 g of the mixed material.

[0175] Test Example 1

[0176] 1 Experimental Animals

[0177] Male SD rats, with a body weight of (200±20) g and 6 weeks old, were purchased from the Animal Science and Technology Center of Jiangxi University of Traditional Chinese Medicine, and the animal license number: SCXK (Gan) 2023-0001. The animals were raised in an environment with a temperature of 25.0±1.5°C and a relative humidity of 45±15%, and the day-night light-dark cycle was 12 / 12 h. After one week of adaptive feeding, the experiment was carried out, and the experimental process followed the relevant regulations on animal welfare and the 3R principle.

[0178] 2 Experiment

[0179] 2.1 Preparation of Drugs

[0180] Guben Yanling Pills solution: Take an appropriate amount of the Guben Yanling Pills prepared in Example 6, cut them into pieces, pulverize them in a pulverizer, and then grind them into a uniform liquid medicine with physiological saline in a mortar. Then, prepare three kinds of liquid medicines with concentrations of 0.8 g / mL, 0.4 g / mL, and 0.2 g / mL respectively, and store them for later use.

[0181] Jinkui Shenqi Pills solution: Take an appropriate amount of Jinkui Shenqi Pills, grind them into a uniform liquid medicine with physiological saline in a mortar, and make a constant volume to prepare a liquid medicine with a concentration of 0.2 g / mL, and store it for later use; among them, the production company of Jinkui Shenqi Pills: Beijing Tongrentang Technology Development Co., Ltd. Pharmaceutical Factory; production batch number: 23031208.

[0182] Vitamin E solution: Take an appropriate amount of water-soluble vitamin E solid powder, stir it with physiological saline in a beaker until there is no solid precipitation and foreign matter, and make a constant volume to 13.5 mg / mL after full dissolution, and store it for later use; among them, water-soluble vitamin E, production company: Beijing Bo'ao Tuoda Technology Co., Ltd.; production batch number: V6010B.

[0183] 2.2 Animal Modeling and Drug Administration

[0184] After 7 days of adaptive feeding, 56 rats were randomly divided into a normal group (Normol), a model group (Model), a Jinkui Shenqi Pills group (JKSQW), and a vitamin E group (Vitamin E 27mg•kg -1), low-dose group of Gubenyanling pills (GBYLW 2g•kg) -1 ), the medium-dose group of Gubenyanling pills (GBYLW 4g•kg) -1 ), High-dose group of Gubenyanling Pills (GBYLW 8g•kg) -1 Eight animals were included in each group. Except for the control group, the other six groups received an intraperitoneal injection of D-galactose (0.5 g / kg). -1 The normal control group was simultaneously injected with an equal volume of physiological saline once daily for 45 consecutive days. Simultaneously with modeling, except for the blank control group and the model group which were given an equal volume of physiological saline by gavage, all other groups were given the corresponding drug solution by gavage at a dose of 10 mL / kg once daily for 60 consecutive days.

[0185] 2.3 Observation and Testing of Indicators

[0186] 2.3.1 General Appearance

[0187] Compared with the normal group, the mental state, hair condition, skin condition, grasping ability, and sensitivity of the model group and each drug administration group were semi-quantitatively scored.

[0188] Table 1. Rats General Condition Assessment Scale

[0189] 2.3.2 Behavioral Experiment Testing

[0190] (1) Tail suspension test

[0191] The rats were suspended 2 cm from the tip of their tails and fixed to a standardized experimental setup, maintaining a vertical distance of 30 cm between their heads and the experimental table surface. A light-blocking baffle was used to isolate the rats' vision. The first 2 minutes were the rats' adaptation period, after which the resting time (in seconds) was continuously recorded for the next 4 minutes.

[0192] (2) Exhaustion Swimming Experiment

[0193] One week before dissection and tissue collection, an exhaustive swimming test was conducted. Each rat was given a lead weight of 10% of its own body weight to swim under load. The lead weight was tied with medical tape 1 cm from the base of the rat's tail and placed in water at a depth of 32 cm and a temperature of (25±1)℃. The exhaustive swimming time (s) was recorded as the rat's inability to float to the surface for 10 seconds.

[0194] 2.3.3 Histopathological examination

[0195] The tissues preserved during the experiment were subjected to histopathological examination using HE (hematoxylin-eosin) staining.

[0196] Tissue embedding and sectioning

[0197] 1) Trimming: Remove each group of kidney tissues from the fixative and trim them in a fume hood. Then rinse them in running water overnight and dehydrate them.

[0198] 2) Dehydration: After rinsing, place the tissue in 70% alcohol for 1 hour, 80% alcohol for 1 hour, 90% alcohol for 1 hour, 90% alcohol for 1 hour, and anhydrous ethanol I and II for 1 hour each.

[0199] 3) Clearing: Immerse the dehydrated tissue in xylene: Xylene I and II for 10 min each.

[0200] 4) Wax impregnation: The transparent tissue was impregnated in paraffin I, II and III at 60℃ for 1 hour each.

[0201] 5) Embedding: Pour liquid paraffin into the embedding mold, then place the fully infiltrated tissue block flat at the bottom, making sure the cut surface is facing down. Then transfer the whole block to a cooling stage to cool. After the paraffin has fully solidified, remove the embedding mold, trim the paraffin block, and store it at room temperature for subsequent sectioning.

[0202] 6) Sectioning and Spreading: Adjust the angle between the section and the blade to be parallel. Then, fix the tissue paraffin block in the sectioning groove. Hold a brush in your left hand and turn the microtome handle with your right hand. After cutting the section, use the brush to support it. The section thickness should be 4μm. Then, gently pick it up with tweezers and place it in a slide spreader at a water temperature of 37℃. After the section is fully spread, hold one end of a glass slide and vertically immerse it in water to attach the section. Then, place the slide on a 60℃ slide oven for 30 minutes to bake.

[0203] HE staining procedure

[0204] 1) Dewaxing to water: After baking the paraffin sections in an oven at 60°C for 1 hour, quickly immerse them in xylene I and II for 5 minutes to dewax. Then hydrate the sections by immersing them in anhydrous ethanol I and II for 5 minutes each, 90% ethanol for 5 minutes, 80% ethanol for 5 minutes, and 70% ethanol for 5 minutes.

[0205] 2) Hematoxylin staining of cell nuclei: The above-mentioned hydrated kidney tissue sections were immersed in hematoxylin staining solution for 5 minutes, then rinsed and soaked with tap water for 5 minutes, then placed in 1% hydrochloric acid alcohol for differentiation for 30 seconds, and then rinsed and soaked with tap water for 5 minutes.

[0206] 3) Eosin staining of cytoplasm: Soak in 70% alcohol for 10 min, soak in 80% alcohol for 10 min, and stain the sections in eosin staining solution for 5 min.

[0207] 4) Dehydration and clearing: Place the slices in 90% alcohol and anhydrous ethanol I for 5 minutes each to remove moisture, then transfer them to xylene I and II for 5 minutes each to clear them.

[0208] Cover photo

[0209] The sections were mounted with neutral resin and preserved. The changes in tissue morphology were observed under a microscope and photographed for preservation.

[0210] 2.4 Animal handling and material collection

[0211] After the experiment, the rats were fasted for 12 hours but allowed free access to water. They were then anesthetized, and blood was drawn from their abdominal aorta to euthanize them.

[0212] Carefully open the abdominal and thoracic cavities, cut open the auricles, and use a syringe to infuse cold saline solution into the apex of the heart until the liver changes from dark red to yellowish-brown or pale white. Then carefully dissect the kidneys and other tissues, removing the surface mucosa and adipose tissue. Wash away blood with saline solution, blot dry with filter paper, and photograph. Take tissue samples from the same location in each group of animals and fix them in 4% paraformaldehyde for histopathological examination.

[0213] 2.5 Statistical Analysis of Data

[0214] Data were processed using SPSS statistical methods, and the results are expressed as mean ± standard deviation (Mean ± SD). One-way ANOVA was used for significance analysis; P > 0.05 indicated no statistical significance, P < 0.05 indicated a statistically significant difference, and P < 0.01 indicated a statistically significant difference.

[0215] 3 Experimental Results

[0216] 3.1 General Appearance

[0217] Observe the mental state, coat condition, skin condition, grasping behavior, and sensitivity of each group of animals daily at regular intervals. For the last 1-2 weeks of the experiment, observe each group of animals using a semi-quantitative scoring scale. Results are detailed in Table 2. Figure 1 Compared with the normal group, the aging degree of the model group was significantly increased (P<0.01); compared with the model group, the appearance of rats in the vitamin E group, the high-dose group of Gubenyanling pill, the medium-dose group of Gubenyanling pill, and the low-dose group of Gubenyanling pill were significantly improved (P<0.05), and the Jinkui Shenqi pill group showed a positive trend.

[0218] Table 2 Comparison of semi-quantitative scores of general appearance of rats in each group

[0219]

[0220] 3.2 Behavioral Experiment Testing

[0221] Table 3 Results of each behavioral experiment

[0222] project Tail suspension test Exhaustion Swimming Experiment normal group 161.3±18.0 398.4±108.2 Model group <![CDATA[188.4±20.8 ## ]]> <![CDATA[253.1±38.0 ## ]]> Jin Kui Shen Qi Wan group <![CDATA[154.1±23.7 ** ]]> 285.7±37.6 Vitamin E group 189.8±8.2 337.8±68.9 High-dose group of Gubenyanling Pills <![CDATA[156.5±11.5 ** ]]> <![CDATA[441.8±137.4 ** ]]> Medium-dose group of Gubenyanling Pill <![CDATA[162.3±19.6 ** ]]> 342.3±55.5 Low-dose group of Gubenyanling Pills 175.1±14.1 188.2±39.3

[0223] 3.2.1 Suspended Tail Experiment

[0224] Table 3 Figure 2 The analysis results showed that, compared with the normal group, the tail suspension rest time of rats in the model group was significantly prolonged (P<0.01), indicating that the aging degree of the animals in the model group was obvious. Compared with the model group, the physical condition of rats in the Jin Kui Shen Qi Wan group, the high-dose Gu Ben Yan Ling Wan group, and the medium-dose Gu Ben Yan Ling Wan group was significantly improved (P<0.01), and the low-dose Gu Ben Yan Ling Wan group showed a positive trend, indicating that the aging process was delayed.

[0225] 3.2.2 Exhaustion Swimming Experiment

[0226] Table 3, Figure 3 The analysis results showed that, compared with the normal group, the exhaustion swimming time of rats in the model group was significantly shortened (P<0.01), indicating that the aging degree of the animals in the model group was obvious; compared with the model group, the physical condition of rats in the high-dose Gubenyanling pill group was significantly improved (P<0.01), and the Jinkui Shenqi pill group, vitamin E group, and medium-dose Gubenyanling pill group showed a positive trend, indicating that the aging process was delayed.

[0227] 3.3 Histopathological examination

[0228] Compared with the normal group, the model group showed significant dilation of Bowman's capsule and marked atrophy and damage to the glomeruli, indicating that the renal tissue of the aging model rats was damaged and their function declined significantly. Compared with the model group, the Jin Kui Shen Qi Wan group, the Vitamin E group, and the high, medium, and low dose groups of Gu Ben Yan Ling Wan all showed significant improvement, indicating that the aging process was delayed. See details. Figure 4-5 (The black arrow points to the renal capsule).

[0229] Test Example 2

[0230] Evaluation of DPPH free radical scavenging ability: The ability to scavenge DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radicals reflects, to some extent, a substance's antioxidant capacity. A higher free radical scavenging rate indicates stronger antioxidant and anti-aging abilities. Therefore, the anti-aging effects of traditional Chinese medicine extracts can be assessed through research on their ability to scavenge DPPH free radicals.

[0231] The herbal extracts prepared in the examples (Extract 1, Extract 2, Extract 3, Extract 4: the sum of extracts 1 and 2 after thorough mixing, Extract 5: the sum of extracts 1 and 3 after thorough mixing, Extract 6: the sum of extracts 1, 2, and 3 after thorough mixing, Extract 7: the sum of extracts 1, 2, and 3 after thorough mixing, Extract 8: the sum of extracts 1, 2, and 3 after thorough mixing, Extract 9: the sum of extracts 1, 2, and 3 after thorough mixing, Extract 10: the sum of extracts 1, 2, and 3 after thorough mixing) were diluted with DMSO to a 2% (w / w) sample for testing. The following test was then performed: 2.0 mL of DPPH test solution (46.7 mg / L) and the sample were added to a test tube. The total volume was increased to 3 mL with anhydrous ethanol. The mixture was shaken well and reacted in the dark for 30 min. The absorbance was then measured at 517 nm using a 1 cm cuvette and recorded as A. 10 Add 2 mL of anhydrous ethanol and the corresponding volume of the sample to be tested to the test tube, and make up the total volume to 3 mL with anhydrous ethanol. Record the measured absorbance as A. 11 Add 2 mL of DPPH test solution and 1 mL of anhydrous ethanol to a test tube, and record the absorbance as A. 12 Calculate the DPPH free radical scavenging rate (Y') of the test solution;

[0232] The formula for calculating the DPPH free radical scavenging rate is:

[0233] In the formula A 10 A represents the absorbance value of the system after DPPH removal from the sample. 11 A represents the absorbance value of the system after DPPH removal from the sample blank control. 12 The absorbance value is the absorbance of the reaction system before the addition of the reagent.

[0234] Table 5

[0235] DPPH free radical scavenging rate / % Example 1 42.5 Example 2 47.4 Example 3 40.2 Example 4 51.3 Example 5 50.1 Example 6 64.7 Example 7 58.8 Example 8 53.6 Example 9 62.1 Example 10 60.9

[0236] As shown in Table 5, the extract prepared in Example 6 exhibits excellent DPPH free radical scavenging performance. This is likely because the medicinal materials were treated separately according to their main active components and content. The introduction of phosphate buffer during water extraction effectively maintains the acid-base stability of the extraction environment, preventing degradation reactions such as hydrolysis, polymerization, and precipitation of heat-sensitive components like saponins, flavonoids, polysaccharides, and proteins due to pH drift, thereby improving the extraction efficiency and stability of the target components. Enzyme protectants were introduced into some raw materials during the enzymatic hydrolysis process to improve the enzyme's activity retention and environmental adaptability during the reaction. Simultaneously, the regulatory factors quercetin-7-O-glucoside and ectoine showed a significant synergistic effect. As a targeted protectant, quercetin-7-O-glucoside can form hydrogen bonds with the enzyme's active site through its glycosidic structure, while its aromatic ring structure inserts into the hydrophobic region of the enzyme, constructing an inactive and stable complex that effectively blocks the entry of non-substrate and the non-selective degradation of key substrates. Ectoyline, by forming a stable hydrated shell microenvironment, protects the enzyme protein conformation and enhances its resistance to adverse factors such as temperature fluctuations and oxidative stress. The combined application of these two agents helps stabilize the enzyme's spatial structure, prolonging its action time, and enhances the specific protection of the target substrate, thereby improving the overall efficiency and precision of the reaction system.

[0237] The preferred embodiments of the present invention have been described in detail above. It should be understood that those skilled in the art can make numerous modifications and variations based on the concept of the present invention without creative effort. Therefore, all technical solutions that can be obtained by those skilled in the art based on the concept of the present invention through logical analysis, reasoning, or limited experimentation on the basis of existing technology should be within the scope of protection defined by the claims.

Claims

1. A method for preparing an anti-aging, longevity-enhancing pill, characterized in that, Includes the following steps: (1) Weigh out the Chinese medicinal materials according to the formula; (2) The following herbs were dried and pulverized: Ophiopogon japonicus, Asparagus cochinchinensis, Rehmannia glutinosa, Rehmannia glutinosa (processed), Dioscorea opposita, Alisma plantago-aquatica, Poria cocos, Achyranthes bidentata, Cornus officinalis, Eucommia ulmoides, Salvia miltiorrhiza, Lycium barbarum, Rubus idaeus, Lycium chinense root bark, fish maw pearl, and pearl. The above-mentioned Chinese herbal powders were then mixed evenly. The above-mentioned Chinese herbal powders were then mixed evenly with solvent and auxiliary agent and heated to obtain extract 1 and filter residue 1, respectively. (3) Ginseng, Polygala tenuifolia, Platycladus orientalis seed, Morinda officinalis (salt-processed), Cistanche deserticola, Lycium barbarum, deer antler glue, Cuscuta chinensis and dog penis are dried and pulverized respectively. Then the above Chinese medicine powders are mixed evenly. The above Chinese medicine powders are added to water, and then enzyme preparations are added. The mixture is heated and the enzymes are inactivated after the treatment. The mixture is filtered to obtain extract 2 and residue 2. (4) Dry and pulverize Schisandra chinensis, Aucklandia lappa, Zanthoxylum bungeanum, Acorus tatarinowii and Lycium barbarum respectively, then mix the above Chinese herbal powders evenly; then perform supercritical CO2 extraction treatment, and after the treatment, filter to obtain extract 3 and residue 3; (5) After sterilizing the above extract 1, extract 2, extract 3, filter residue 1, filter residue 2, and filter residue 3 respectively, mix them evenly to obtain a mixture. (6) Mix the above mixture with the auxiliary materials, refine the honey and make pills to obtain anti-aging and longevity pills.

2. The preparation method of the anti-aging and longevity-enhancing pill as described in claim 1, characterized in that: The solvent is phosphate buffer.

3. The preparation method of the anti-aging and longevity-enhancing pill as described in claim 1, characterized in that: The auxiliary agent is mannitol.

4. The preparation method of the anti-aging and longevity-enhancing pill as described in claim 1, characterized in that: Step (3) also includes enzyme protectants and regulatory factors.

5. The preparation method of the anti-aging and longevity-enhancing pill as described in claim 1, characterized in that: The enzyme preparation is at least one of cellulase, protease, and pectinase.

6. The preparation method of the anti-aging and longevity-promoting pill as described in claim 4, characterized in that: The enzyme protectant is at least one of mannitol, sorbitol, and trehalose.

7. The preparation method of the anti-aging and longevity-promoting pill as described in claim 1, characterized in that: The dosage of each ingredient is as follows: 60g ginseng, 60g ophiopogon japonicus, 60g schisandra chinensis, 60g asparagus cochinchinensis, 60g rehmannia glutinosa, 60g prepared rehmannia glutinosa, 60g yam, 30g alisma plantago-aquatica, 60g poria cocos, 60g achyranthes bidentata, 60g cornus officinalis, 60g eucommia ulmoides, 60g costus root, 30g polygala tenuifolia, 60g arborvitae seed, 60g morinda officinalis (salt-processed), 30g zanthoxylum bungeanum, 30g acorus tatarinowii, 120g salvia miltiorrhiza, 120g cistanche deserticola, 45g lycium barbarum, 45g raspberry, 45g lycium chinense root bark, 30g deer antler glue, 120g cuscuta chinensis, 30g fish maw pearl, 9g pearl, and 25g dog penis.

8. The preparation method of the anti-aging and longevity-promoting pill as described in claim 2, characterized in that: The pH of the phosphate buffer solution is 6.8-7.

2.

9. The preparation method of the anti-aging and longevity-enhancing pill as described in claim 4, characterized in that: The regulatory factor is at least one of quercetin-7-O-glucoside and ectoine.

10. An anti-aging tonic pill for strengthening the body and prolonging life, characterized in that: Prepared by the preparation method according to any one of claims 1-9.

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