Broiler chicken staged combined feed additive as well as use method and application of broiler chicken staged combined feed additive

Abstract

The invention discloses a broiler chicken staged combined feed additive, a using method and application, and belongs to the technical field of poultry breeding, the broiler chicken staged combined feed additive comprises a first-stage additive and a second-stage additive; the first-stage additive comprises a bacillus amyloliquefaciens TL preparation, and the second-stage additive comprises a lactobacillus gasseri preparation. Through combined application of the two strains, expression of fat synthesis related genes is promoted, host lipid metabolism is regulated and controlled, the growth performance of the broiler chickens can be remarkably improved, and the method has great significance in improving the breeding efficiency of the broiler chickens.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of poultry breeding, and particularly relates to a broiler feed additive used in different stages, a use method and application. BACKGROUND

[0002] In modern animal husbandry, probiotics are increasingly widely used in broiler breeding as green substitutes for antibiotics. Bacillus amyloliquefaciens TL is a highly efficient probiotic isolated from the body of Tongcheng pigs. Previous studies have shown that it can significantly promote the growth of white-feathered broilers in the early stage (1-21 days old), but the growth promotion effect is significantly reduced in the 22-35 day old stage, which limits its application effect in the whole growth cycle of broilers.

[0003] At present, the application of single probiotics in animal breeding has limitations, especially the insufficient sustained promotion of growth performance in the later growth stage of broilers. The existing research has not yet understood how probiotics regulate the intestinal flora of broilers. How to reasonably match probiotics to make up for the performance defects of single strains has become a key problem to improve the efficiency of broiler breeding. SUMMARY

[0004] Therefore, the application discloses a broiler feed additive used in different stages, a use method and application.

[0005] The application adopts the following technical scheme:

[0006] A broiler feed additive used in different stages, the additive comprising a stage one additive and a stage two additive; the stage one additive comprising a Bacillus amyloliquefaciens TL preparation, and the stage two additive comprising a Lactobacillus gasseri preparation.

[0007] Further, the number of viable bacteria in the Bacillus amyloliquefaciens TL preparation is greater than or equal to 1.0x10 10 CFU / g.

[0008] Further, the number of viable bacteria in the Lactobacillus gasseri preparation is greater than or equal to 1.0x10 9 CFU / kg.

[0009] Further, the addition amount of the Bacillus amyloliquefaciens TL preparation in the feed is 200 mg / kg.

[0010] Further, the addition amount of the Lactobacillus gasseri preparation in the feed is 1000 mg / kg.

[0011] A use method of the above-mentioned feed additive, the Bacillus amyloliquefaciens TL preparation is used for feed addition in the early stage of broilers, and the Lactobacillus gasseri preparation is used for feed addition in the later stage of broilers.

[0012] Further, the early stage comprises 1-21 days of age.

[0013] Further, the late stage comprises 22-35 days of age.

[0014] An application of the above-mentioned feed additive for improving sugar lipid metabolism of broilers.

[0015] Further, the sugar lipid metabolism comprises: significantly improving the levels of very low density lipoprotein, triglyceride, free fatty acid and blood glucose in serum of broilers, and up-regulating the expression of insulin-like growth factor-1; promoting fat synthesis and transport, and accelerating gluconeogenesis.

[0016] The source of the probiotic preparation involved in the technical scheme of the present application is explained that Bacillus amyloliquefaciens TL was sent to the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan City, Hubei Province on June 4, 2015, and the preservation number thereof is CCTCC NO: M 2015359, and the preservation center telephone is 027-68754052, and Bacillus amyloliquefaciens TL can be obtained from the preservation center or purchased from Hubei Huada Rui'er Technology Co., Ltd., and the Lactobacillus gasseri preparation is a conventional preparation and can be obtained through a conventional purchase route.

[0017] The beneficial effects of the present application are:

[0018] Growth performance improvement: the broiler stage-by-stage combined feed additive disclosed in the present application can significantly improve the body weight, daily weight gain and daily weight gain rate of broilers at 22-35 days of age by feeding Bacillus amyloliquefaciens TL preparation and Lactobacillus gasseri preparation to broilers at different stages, adding Bacillus amyloliquefaciens TL in the early stage and adding Lactobacillus gasseri in the late stage, so as to significantly improve the broiler breeding efficiency. As shown in Example 2, compared with the late-stage diet control group without addition, the daily weight gain increased by 33.3%, and the feed-to-weight ratio decreased by 22.1%.

[0019] Metabolic regulation strengthening: the stage-by-stage combination of the two probiotics can regulate the serum sugar lipid metabolism index, significantly improve the levels of very low density lipoprotein (VLDL), triglyceride (TG), free fatty acid (FFA) and blood glucose (GLU) in serum of broilers, and up-regulate the expression of insulin-like growth factor-1 (IGF-1). It can promote fat synthesis and transport, accelerate gluconeogenesis, realize precise regulation of energy metabolism, and has important significance for improving broiler breeding efficiency. DETAILED DESCRIPTION

[0020] In order to better understand the technical scheme of the present application, the examples of the present application are described in detail as follows.

[0021] It should be noted that the embodiments described are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0022] Embodiment 1

[0023] A broiler chicken stage-specific combined feed additive, the additive comprising a stage one additive and a stage two additive; the stage one additive comprising a Bacillus amyloliquefaciens TL preparation, and the stage two additive comprising a Lactobacillus gasseri preparation.

[0024] Further, the viable bacterial count of the Bacillus amyloliquefaciens TL preparation is ≥1.0×10 10 CFU / g.

[0025] Further, the viable bacterial count of the Lactobacillus gasseri preparation is ≥1.0×10 9 CFU / kg.

[0026] Further, the Bacillus amyloliquefaciens TL preparation is added to the feed at an amount of 200 mg / kg.

[0027] Further, the Lactobacillus gasseri preparation is added to the feed at an amount of 1000 mg / kg.

[0028] A method for using the above-mentioned feed additive, the Bacillus amyloliquefaciens TL preparation being used for feed addition in the early stage of broiler chickens, and the Lactobacillus gasseri preparation being used for feed addition in the later stage of broiler chickens.

[0029] Further, the early stage comprises 1-21 days of age.

[0030] Further, the later stage comprises 22-35 days of age.

[0031] An application of the above-mentioned feed additive, the additive being used for improving the glycolipid metabolism of broiler chickens.

[0032] Further, the glycolipid metabolism comprises: significantly increasing the levels of very low density lipoprotein, triglyceride, free fatty acid and blood glucose in the serum of broiler chickens, and up-regulating the expression of insulin-like growth factor-1; promoting fat synthesis and transport, and accelerating gluconeogenesis.

[0033] Embodiment 2

[0034] 1. Effect of stage-specific feeding on the growth performance of white-feathered broiler chickens

[0035] Male white-feathered broilers were selected for the experiment, and there was no statistically significant difference in the initial body weight of each broiler at 1 day old. Each group had 3 replicates, with 8 chickens in each replicate. During the experiment, the standardization cage raising mode was used, with a feeding density of 10-12 chickens per cage, and the feed and water were always sufficient. The daily feed intake was recorded, and the individual body weight and health status were measured every week. The basic diet was produced and processed by Nanchang Economic and Technological Development Zone Faith Feed Store, and the basic diet formula is shown in Table 1. The number of viable bacteria in Bacillus amyloliquefaciens TL preparation was 1.0×10 10 CFU / g; the number of viable bacteria in Lactobacillus gasseri, Pediococcus acidilactici, and Lactobacillus plantarum preparation was 1.0×10 9 CFU / kg. The Pediococcus acidilactici and Lactobacillus plantarum preparations were conventional preparations and could be obtained through conventional purchase channels.

[0036] Table 1 Basic diet formula

[0037]

[0038] The grouping of white-feathered broilers in the experiment is shown in Table 2, and Table 3 shows the weekly body weight of white-feathered broilers from 1-21d, and Table 4 shows the growth performance of white-feathered broilers from 21d-35d.

[0039] Table 2 Feeding situation of experimental groups of chickens

[0040]

[0041] Table 3 Weekly body weight of white-feathered broilers from 1-21d (unit: g)

[0042]

[0043] Table 4 Growth performance of white-feathered broilers from 21d-35d

[0044]

[0045] The body weight of the four groups of broilers in the baseline period (1-21d) showed a typical growth trend, and there was no statistically significant difference between the groups, indicating that the body weight of the broilers remained consistent during this period. During the period of 21-35d, the average weight gain of G group, P group, and Z group was 33.33%, 29.14%, and 32.66% higher than that of C group, respectively; and the feed conversion ratio was reduced by 22.13%, 20.00%, and 17.45%, respectively. Therefore, blood indicators were measured for G group and C group.

[0046] 2. Effect of feeding at different stages on blood indicators

[0047] The indicators of glycolipid metabolism, including very low density lipoprotein (VLDL), insulin (INS), growth hormone (GH) and insulin-like growth factor 1 (IGF-1), were determined by Elisa kit according to the instructions of the kit. The serum glucose (GLU), free fatty acid (FFA) and triglyceride (TG) were determined by the corresponding instruments.

[0048] The serum biochemical test showed that the intervention group of Lactobacillus gasseri (G group) achieved systematic metabolic upgrading compared with the control group (C group): the blood glucose (GLU-YA) level was significantly increased by 36.0%, laying the foundation for the energy supply of the body; the free fatty acid (FFA) and triglyceride (TG) were increased by 57.1% and 31.4%, respectively, and the very low density lipoprotein (VLDL) showed a 16.1% upward trend, confirming the dual enhancement of lipid mobilization and synthesis; the insulin-like growth factor-1 (IGF-1) was increased by 19.8%, directly driving growth and development.

[0049] The effects of Lactobacillus gasseri on the glycolipid metabolism and growth regulation of broilers were evaluated by determining the serum biochemical and growth-related indicators. In terms of lipid metabolism, the second phase of feeding Lactobacillus gasseri additives significantly increased the level of very low density lipoprotein (VLDL), suggesting that it may promote lipid metabolism by enhancing liver lipid synthesis or transport mechanism. In addition, the triglyceride (TG) level of G group was significantly higher than that of the control group, and the concentration of free fatty acid (FFA) also increased significantly, indicating that Lactobacillus gasseri may affect energy metabolism by inhibiting fat decomposition or enhancing lipid accumulation pathway. In terms of sugar metabolism, the blood glucose (GLU-YA) level of G group was significantly higher than that of the control group, but there was no significant difference in insulin (Insulin) concentration between the two groups, suggesting that several probiotics may inhibit glycolysis and promote gluconeogenesis. The analysis of growth-related hormones showed that the insulin-like growth factor-1 (IGF-1) level of G group was significantly higher than that of the control group, while the growth hormone (GH) had no significant difference between the two groups, indicating that Lactobacillus gasseri may promote the growth of broilers by activating the IGF-1 synthesis pathway rather than directly regulating GH secretion.

[0050] Table 5 Serum biochemical indicators at 35 days of age

[0051]

[0052] Example 3

[0053] In order to study the effects of the technical scheme of feeding Bacillus amyloliquefaciens TL at 1-21 days of age and Lactobacillus gasseri at 22-35 days of age on the growth performance of white-feathered broilers, the following tests were conducted in the breeding farm

[0054] 1. Experimental materials and methods

[0055] 1.1 Experimental animals and feeding

[0056] In a certain breeding base in Hubei Province, 9000 male white-feather broilers were selected for the test, and there was no statistically significant difference in the initial body weight of each broiler at 1 day old. Each group had 3 replicates, and each replicate had 500 chickens. During the test period, the standardized cage raising mode was adopted, the feeding density was 10-12 chickens per cage, and the feed and drinking water were always sufficient. The daily feed intake was recorded, and the individual body weight and health status were measured every week. The diet, Bacillus amyloliquefaciens TL, and Lactobacillus gasseri were the same as in Example 2.

[0057] Table 6 Feeding situation of test group chickens

[0058]

[0059] During the test period, the standardized cage raising mode was adopted, the feeding density was 10-12 chickens per cage, and the feed and drinking water were always sufficient. The daily feed intake was recorded, and the individual body weight and health status were measured every week.

[0060] Average daily gain (ADG) = (final weight - initial body weight) / test days

[0061] Average daily feed intake (AFDI) = feed intake / (test days x number of test chickens)

[0062] Feed / gain rate (F / G) = average daily feed intake / average daily gain.

[0063] Table 7 Growth performance of 1-35 day old white-feather broilers

[0064]

[0065] The results in Table 7 show that compared with the control group, the white-feather broilers in test groups 1-5 increased in weight by 29.69%, 28.43%, 14.61%, 9.54%, and 15.70%, respectively; the average daily feed intake increased by 9.94%, 9.88%, 6.34%, 4.77%, and 5.72%, respectively; and the feed / gain ratio decreased by 15.34%, 14.20%, 7.39%, 4.55%, and 8.52%, respectively. The effect of adding Bacillus amyloliquefaciens TL during 1-21 days and Lactobacillus gasseri during 22-35 days was better, and the effect of adding 500 mg / kg and 1000 mg / kg during 22-35 days was less different. This experiment shows that the effect of adding Bacillus amyloliquefaciens TL in the early stage (1-21 d) and Lactobacillus gasseri in the later stage (22-35 d) on the improvement of production performance is better than that of adding Lactobacillus gasseri in the early stage and Bacillus amyloliquefaciens TL in the later stage, or simultaneously adding the mixed preparation of Bacillus amyloliquefaciens TL and Lactobacillus gasseri without staging, indicating that the present application has better effect when the feed is used in stages and in order.

[0066] The above has carried out the detailed introduction to the embodiment of the application, the principle and implementation mode of the application have been described by applying specific examples in this paper, the above embodiment description is only used for helping understanding the method of the application and its core idea; at the same time, for the general technical personnel in the art, according to the idea of the application, there will be changes in specific implementation mode and application range, and the above is described, the content of the specification should not be understood as the limitation of the application.

Claims

1. A staged feed additive for broilers, characterized in that, The additives include a Phase 1 additive and a Phase 2 additive; the Phase 1 additive includes Bacillus amyloliquefaciens TL preparation, and the Phase 2 additive includes Lactobacillus gasseri preparation.

2. The feed additive according to claim 1, characterized in that, The viable count of the Bacillus amyloliquefaciens TL preparation is ≥1.0×10⁻⁶. 10 CFU / g.

3. The feed additive according to claim 1, characterized in that, The viable count of the Lactobacillus gasseri preparation is ≥1.0 × 10⁻⁶. 9 CFU / kg.

4. The feed additive according to claim 2, characterized in that, The dosage of the Bacillus amyloliquefaciens TL preparation added to the feed is 200 mg / kg.

5. The feed additive according to claim 3, characterized in that, The dosage of the Lactobacillus gasseri preparation added to the feed is 1000 mg / kg.

6. A method of using the feed additive as described in any one of claims 1-5, characterized in that, The Bacillus amyloliquefaciens TL preparation is used as a feed additive for broilers in the early stage; the Lactobacillus gasseri preparation is used as a feed additive for broilers in the later stage.

7. The method of use according to claim 6, characterized in that, The early stage includes ages 1-21 days.

8. The method of use according to claim 7, characterized in that, The later stage includes 22-35 days of age.

9. The use of a feed additive as described in any one of claims 1-5, wherein the additive is used to improve glucose and lipid metabolism in broilers.

10. The application according to claim 9, characterized in that, The glucose and lipid metabolism includes promoting fat synthesis and transport, and accelerating gluconeogenesis.

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