Composition with soothing and repairing effects as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetic raw materials, in particular to a composition with soothing and repairing effects and a preparation method and application thereof.The composition is prepared from, by mass, 0.1%-1.0% of madecassoside, 0.1%-1.0% of meadowfoam seed oil, 1.0%-3.0% of calendula extract, 0.1%-0.3% of allantoin, 0.01%-0.05% of hydroxyethyl cellulose, 0.1%-0.3% of carbomer 981, 1%-5% of tween-20 surfactant, 1%-5% of essence and the balance deionized water. The madecassoside, the meadowfoam seed oil and the calendula extract are scientifically compounded to achieve a remarkable synergistic effect by taking the madecassoside, the meadowfoam seed oil and the calendula extract as the core active ingredients, adding the madecassoside, the meadowfoam seed oil and the calendula extract as the core active ingredients, uniformly mixing the madecassoside, the meadowfoam seed oil and the calendula extract to form the water-based bactericidal composition, and sterilizing the madecassoside, the meadowfoam seed oil and the calendula extract to form the water-based bactericidal composition.

Description

Technical Field

[0001] This invention relates to the field of cosmetic raw material technology, and more specifically, to compositions with soothing and repairing effects, preparation methods, and applications. These compositions are particularly suitable for soothing and repairing sensitive skin and can be widely used in the formulation of cleansing and skincare products. Background Technology

[0002] With the rapid development of modern society, environmental pollution is becoming increasingly severe. Air pollutants, ultraviolet radiation, and various chemicals cause continuous damage to human skin. Both facial and scalp skin are susceptible to external adverse factors. Prolonged exposure to these irritants gradually impairs the skin barrier function, increases the release of inflammatory factors, and subsequently triggers various dermatitis symptoms or allergic reactions. This inflammatory state not only affects appearance but also further deteriorates the skin barrier function, creating a vicious cycle.

[0003] In the field of cosmetic research and development, most current research focuses on developing functional products such as whitening and spot removal, while products targeting skin inflammation suppression and soothing repair are relatively few. Although some products containing anti-inflammatory ingredients exist on the market, these products often suffer from high costs, limited efficacy, or poor stability, making it difficult to meet the growing skincare needs of people with sensitive skin.

[0004] Centella asiatica, a plant of the Apiaceae family, has long been recognized for its medicinal value in traditional Chinese medicine texts. Modern pharmacological research shows that asiaticoside, an alpha-caryophyllein extract, is the core component responsible for its anti-inflammatory and repairing functions. Aydasiaticoside can block the skin inflammatory response induced by the pro-inflammatory cytokine interleukin-1β by inhibiting the translocation of Toll-like receptor 2 and nuclear factor κB, demonstrating promising applications in promoting wound healing, anti-inflammation, skin barrier repair, and anti-oxidation. Meadowfoam seed oil, renowned for its excellent stability and skin-care properties, is considered one of the most stable plant oils. Its rich content of long-chain fatty acids, natural tocopherols, and phytosterols endows it with superior antioxidant properties, deep moisturizing ability, and barrier repair function. Calendula extract, a traditional folk remedy, contains abundant carotenoids, flavonoids, and triterpenoid saponins, exhibiting significant effects in soothing inflammation, anti-oxidation, and whitening and repairing.

[0005] While the aforementioned natural active ingredients each possess excellent skincare benefits, their practical application still faces numerous challenges. In particular, asiaticoside, as a highly effective anti-inflammatory and repairing ingredient, has a high production cost. From an economic perspective, adding high concentrations of asiaticoside significantly increases product costs, hindering market promotion. Conversely, adding only trace amounts of asiaticoside often fails to fully realize its soothing and repairing effects, making it difficult to meet consumer expectations. This conflict between cost and efficacy is a major obstacle restricting the widespread application of asiaticoside. Summary of the Invention

[0006] To address the aforementioned problems in the existing technology, the primary objective of this invention is to provide a composition with soothing and repairing effects. This composition scientifically combines asiaticoside with meadowfoam seed oil and calendula extract in a specific ratio. Utilizing the synergistic effect between multiple active ingredients, it achieves a soothing and repairing effect greater than the simple sum of the individual components while reducing the concentration of asiaticoside used. This effectively solves the technical challenges of high cost and insignificant effects at low concentrations when using single-ingredient formulations.

[0007] Another object of the present invention is to provide a method for preparing the above-mentioned composition with soothing and repairing effects. This preparation method has a simple process flow, is convenient to operate, has low production costs, requires no special equipment, and produces stable and controllable product quality, making it suitable for large-scale industrial production.

[0008] Another object of the present invention is to provide the application of the above-described composition with soothing and repairing effects in cosmetics. This composition can be widely used in the formulation of washing and care products and skin care products, and is particularly suitable for soothing and repairing sensitive skin.

[0009] To achieve the above-mentioned objectives, the technical solution adopted by the present invention is as follows: This invention provides a composition with soothing and repairing effects, comprising the following components by weight percentage: 0.1%-1.0% asiaticoside, 0.1%-1.0% meadowfoam seed oil, 1.0%-3.0% calendula extract, 0.1%-0.3% allantoin, 0.01%-0.05% hydroxyethyl cellulose, 0.1%-0.3% carbomer 981, 1%-5% Tween-20 surfactant, 0.1%-0.3% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 5.0%-10% 1,3-butanediol, with deionized water as the balance to 100%.

[0010] In a preferred embodiment of the present invention, the content of asiaticoside is 0.55%, the content of meadowfoam seed oil is 0.55%, and the content of calendula extract is 2.0%. This ratio was determined through extensive experimental optimization, which can effectively control costs while ensuring soothing and repairing efficacy.

[0011] Furthermore, the asiaticoside is preferably derived from Centella asiatica extract, wherein the content of asiaticoside in the Centella asiatica extract is 90%. Using high-purity Centella asiatica extract as the source of asiaticoside not only ensures the stability of the active ingredient but also simplifies the formulation design and production process.

[0012] In this invention, the main components of meadowfoam seed oil include long-chain fatty acids, natural tocopherols, and phytosterols. These components work synergistically to endow meadowfoam seed oil with excellent antioxidant properties, deep moisturizing ability, and skin barrier repair function. Meanwhile, the main components of calendula extract include carotenoids, flavonoids, triterpenoid saponins, volatile oils, phytosterols, and polysaccharides. These active ingredients play important roles in soothing inflammation, promoting repair, anti-oxidation, and moisturizing.

[0013] The composition prepared in this invention exhibits a stable emulsion state with good fluidity and spreadability. Testing showed that this composition inhibits hyaluronidase activity by 54%-63%, significantly higher than comparative samples containing only a single component or with incomplete components. Hyaluronidase, as a participant in allergic reactions, is closely related to the release of inflammatory mediators such as histamine from mast cells; therefore, inhibiting hyaluronidase activity is an important indicator for evaluating soothing efficacy. Simultaneously, the composition resulted in a 148%-163% proliferation rate in NIH / 3T3 cells, indicating its significant ability to promote cell proliferation and wound healing.

[0014] The present invention also provides a method for preparing the above-mentioned composition with soothing and repairing effects, the method comprising the following steps: Step 1: Dissolve the Centella asiatica extract in deionized water at room temperature to obtain phase A. The content of asiaticoside in the Centella asiatica extract is 90%. Dissolving at room temperature can avoid the destruction of the activity of asiaticoside by high temperature and ensure the stability of the component.

[0015] Step two involves adding allantoin, hydroxyethyl cellulose, and carbomer 981 to hot water for complete swelling to obtain phase B. Preferably, the temperature of the hot water is 70°C to 90°C, and the swelling time is 20 to 40 minutes. Sufficient swelling of the thickener is crucial for forming a stable emulsion system; appropriate temperature and time ensure complete hydration and molecular chain unfolding of the thickener, thereby achieving optimal thickening and stabilizing effects.

[0016] Step 3: Mix phase A and phase B thoroughly, then add Tween-20 surfactant, triethanolamine, calendula extract, and the remaining deionized water sequentially, stirring until homogeneous to obtain phase C. In this step, triethanolamine acts as a pH adjuster, reacting with carbomer to bring the system pH to a suitable range, while simultaneously promoting the full unfolding of carbomer to form a three-dimensional network structure.

[0017] Step four: Add p-hydroxyacetophenone to 1,2-hexanediol and heat and stir at 60°C to 90°C until the p-hydroxyacetophenone is completely dissolved. After cooling, add 1,3-butanediol and mix thoroughly. Add the resulting mixture to phase C and stir until homogeneous to obtain phase D. As a preservative, p-hydroxyacetophenone needs to be completely dissolved under heating conditions to ensure its uniform dispersion in the system and to exert a stable preservative effect.

[0018] Step 5: Place the D phase in a homogenizer, and slowly add meadowfoam seed oil during the homogenization and dispersion process. Continue homogenization and emulsification until a stable emulsion system is formed to obtain the composition with soothing and repairing effects. Preferably, the homogenizer speed is 8000 rpm to 12000 rpm, the homogenization and emulsification time is 15 minutes to 30 minutes, and the meadowfoam seed oil is added at a rate of 5% to 10% of the total amount per minute. By controlling the homogenization parameters and the oil phase addition rate, an emulsion product with uniform particle size and good stability can be obtained.

[0019] This invention also provides the application of the above-mentioned composition with soothing and repairing effects in the preparation of cosmetics. The cosmetics can be personal care products or skin care products, wherein personal care products include, but are not limited to, shampoos, conditioners, and shower gels, and skin care products include, but are not limited to, various product forms such as face creams, lotions, serums, and masks.

[0020] In practical applications, the composition is added to cosmetics at a rate of 1% to 10% of the total mass of the cosmetic. This composition exerts a soothing and repairing effect by inhibiting the skin inflammatory response induced by the pro-inflammatory cytokine interleukin-1β, and is particularly suitable for soothing and repairing care of sensitive skin.

[0021] Compared with the prior art, the present invention has the following significant advantages: First, this invention achieves a significant synergistic effect by scientifically combining three core active ingredients: asiaticoside, meadowfoam seed oil, and calendula extract. Asiaticoside blocks inflammatory responses by inhibiting the Toll-like receptor 2 and nuclear factor κB signaling pathways; meadowfoam seed oil, rich in natural tocopherols, provides powerful antioxidant protection and repairs damaged skin barriers; and flavonoids and triterpenoid saponins in calendula extract further enhance the anti-inflammatory and soothing effects. The three ingredients synergistically work on multiple levels, including anti-inflammatory, antioxidant, and barrier repair, making the overall efficacy of the composition significantly superior to the simple additive effect of using each ingredient alone. Experimental data show that the embodiment containing all three active ingredients achieved an inhibition rate of 54%-63% on hyaluronidase, while the comparative embodiment lacking any one of the core ingredients only achieved an inhibition rate of 27%-44%, fully demonstrating the superiority of the synergistic effect.

[0022] Secondly, this invention effectively resolves the cost-efficacy contradiction inherent in using asiaticoside alone. By combining it with meadowfoam seed oil and calendula extract, excellent soothing and repairing effects can be achieved with an asiaticoside concentration of only 0.1%-1.0%, compared to the 3%-5% required for asiaticoside alone, reducing costs by approximately 60%-80%. This formulation strategy not only ensures product efficacy but also significantly enhances the product's economic viability and market competitiveness.

[0023] Third, the composition prepared by this invention is in a stable emulsion state, which facilitates direct addition and dispersion in various cosmetic formulations without the need for additional dissolution or emulsification steps, greatly simplifying the production process of downstream products. Meanwhile, the thickeners and emulsifiers in the composition have been carefully selected and optimized in proportion, ensuring the long-term stability of the product under different temperature and pH conditions, with a shelf life of over 24 months.

[0024] Fourth, the compositions of this invention exhibit excellent performance in promoting cell proliferation and wound healing. NIH / 3T3 cell proliferation rate testing showed that the examples containing the complete active ingredients achieved a cell proliferation rate of 148%-163%, with 3874-5523 ​​cells entering the wound after 24 hours, compared to only 126%-139% and 2578-2872 cells in the control group. This indicates that the compositions of this invention not only inhibit inflammatory responses but also actively promote the repair and regeneration process of damaged skin, achieving a dual effect of soothing and repair.

[0025] Fifth, the preparation method of this invention is simple, convenient to operate, and requires no special equipment. The entire preparation process does not require extreme conditions such as high temperature and high pressure; the components can be dissolved, mixed, and emulsified under normal pressure, resulting in low energy consumption and high safety. The production process is easy to control, the product quality is stable, and it is suitable for large-scale industrial production. Furthermore, the raw materials are widely available and easy to procure, further reducing production costs.

[0026] Sixth, the composition of this invention exhibits excellent safety. Human skin patch tests verified that all 15 subjects showed negative skin reactions, with no irritation or allergic reactions. This is due to the fact that all ingredients in the formula are of natural plant origin or commonly used safe ingredients in cosmetics, and the dosage of each ingredient has been scientifically optimized to ensure both efficacy and safety. The product is particularly suitable for people with sensitive skin and can be used safely for a long time.

[0027] Seventh, the composition of this invention has a wide range of applications, and the dosage can be flexibly adjusted according to different product needs. Adding 1%-3% to washing and care products can impart soothing and repairing functions, while adding 3%-10% to skin care products can achieve stronger anti-inflammatory and repairing effects. This flexibility allows the composition of this invention to meet multi-level needs from daily care to functional products. Attached Figure Description

[0028] Figure 1 This is a comparison chart of the soothing and repairing effects of the embodiments of the present invention and the blank control, demonstrating the significant effects of the samples containing the composition of the present invention on skin soothing and repair.

[0029] Figure 2 The diagram shows the composition product prepared according to the embodiments of the present invention, illustrating the stable emulsion state and good appearance properties of the composition. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in detail below with reference to specific embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of protection of this invention. Those skilled in the art, guided by the technical solutions of this invention, can make various modifications and variations, all of which should fall within the scope of protection of the claims of this invention.

[0031] In the following embodiments, unless otherwise specified, all percentages are by mass and all temperatures are in Celsius. The raw materials used in the embodiments are from the following sources: Centella asiatica extract was purchased from a domestic commercial raw material supplier. The asiaticoside content was determined to be 90% by high-performance liquid chromatography. It is a pale yellow powder, easily soluble in water and polyols. Meadowfoam seed oil was purchased from Xianting Trading Co., Ltd. It is a pale yellow, transparent oily liquid with a characteristic odor; its main fatty acid composition is erucic acid and oleic acid. Calendula officinalis extract was purchased from Bolton Biotechnology Co., Ltd. It is an orange-yellow to brownish-yellow powder or liquid, rich in carotenoids and flavonoids. Tween-20 was purchased from Guangdong Runhua Chemical Co., Ltd. Its chemical name is polyoxyethylene sorbitan monolaurate, a nonionic surfactant. Carbomer 981 was purchased from Lubrizol Corporation. It is a white, loose powder, a high-molecular-weight cross-linked acrylic resin. Hydroxyethyl cellulose was purchased from Ashland Investment Co., Ltd. It is a white to slightly yellow fibrous or powdery solid with good thickening and stabilizing properties. Allantoin was purchased from Guangzhou Hemiao Biotechnology Co., Ltd. Its chemical name is 5-ureidoylhydantoin, a white crystalline powder. Triethanolamine was purchased from Dow Chemical Company; it is a colorless to pale yellow transparent viscous liquid with an ammonia odor. p-Hydroxyacetophenone was purchased from Beijing Tianhong Tianda Technology Co., Ltd.; it is a white crystalline powder with antiseptic and antibacterial properties. 1,2-Hexanediol was purchased from CM Bridge; it is a colorless transparent liquid with both moisturizing and antiseptic properties. 1,3-Butanediol was purchased from Shandong Haike Xinyuan Materials Technology Co., Ltd.; it is a colorless transparent viscous liquid widely used as a cosmetic moisturizer. Deionized water was prepared in the laboratory; its conductivity is less than 2 μSiemens / cm.

[0032] Example 1: Preparation of a soothing and repairing composition This embodiment prepares a composition with soothing and repairing effects. The composition contains the following components by mass percentage: Centella asiatica extract 0.1%, Calendula officinalis extract 1.0%, meadowfoam seed oil 0.1%, Tween-20 1.0%, Carbomer 981 0.1%, hydroxyethyl cellulose 0.01%, allantoin 0.1%, triethanolamine 0.1%, p-hydroxyacetophenone 0.5%, 1,2-hexanediol 0.5%, 1,3-butanediol 5.0%, and deionized water to 100%.

[0033] The preparation method of this embodiment includes the following steps: First, weigh 0.1 g of Centella asiatica extract and add it to 50 ml of deionized water. Stir at 300 rpm for 30 minutes at room temperature using a magnetic stirrer until the Centella asiatica extract is completely dissolved, resulting in a transparent to slightly yellow phase A solution. The advantage of room temperature dissolution is that it avoids the destruction of the active ingredient asiaticoside by high temperatures, and also reduces energy consumption.

[0034] Next, in another container, add 30 ml of deionized water and heat to 80°C. Then, add 0.1 g of allantoin, 0.01 g of hydroxyethyl cellulose, and 0.1 g of carbomer 981 sequentially. When adding the thickeners, use a dispersion method, slowly sprinkling the powder into the water while stirring to prevent clumping. Continue stirring at 80°C for 30 minutes to allow all components to fully swell and hydrate, forming a viscous B-phase gel. Allantoin completely dissolves at this temperature, and the molecular chains of both thickeners fully expand, laying the foundation for the subsequent formation of a stable emulsion system.

[0035] Subsequently, the A-phase solution was slowly added to the B-phase gel while stirring to ensure thorough mixing of the two phases. After homogeneous mixing, 1.0 g of Tween-20, 0.1 g of triethanolamine, and 1.0 g of calendula extract were added sequentially, and stirring continued for 15 minutes. During this process, triethanolamine and carbomer underwent a neutralization reaction, raising the pH of the system from approximately 3 to 6-7. The carboxyl groups of the carbomer ionized to form a negative charge, and the electrostatic repulsion between the molecular chains caused the polymer to fully extend, resulting in a significant increase in the viscosity of the system. The remaining deionized water was added to adjust to the predetermined volume, yielding a homogeneous C-phase.

[0036] Next, add 0.5 g of 1,2-hexanediol to a small beaker and heat to 70°C. Add 0.5 g of p-hydroxyacetophenone and heat with continuous stirring until the p-hydroxyacetophenone is completely dissolved and the solution becomes transparent. After cooling to 40°C, add 5.0 g of 1,3-butanediol and mix thoroughly. Slowly add this mixture to phase C while stirring to ensure uniform dispersion of the preservative and moisturizing system, thus obtaining phase D.

[0037] Finally, the D phase was transferred to a container equipped with a high-shear homogenizer, and the homogenizer was started at a speed of 10,000 rpm. During homogenization, 0.1 g of meadowfoam seed oil was slowly added dropwise to the system at a rate of 6% of the total oil phase per minute using a peristaltic pump. The slow addition of the meadowfoam seed oil and the high-speed shear action ensured that the oil droplets were fully dispersed and encapsulated in the interfacial film formed by the emulsifier, resulting in a stable emulsion system. After continuous homogenization for 20 minutes, a milky white, fine-textured, and highly fluid soothing and repairing composition was obtained. After standing for 24 hours, no stratification or precipitation was observed in the composition, and the oil droplets were uniform in size, distributed in the range of 1-5 micrometers.

[0038] Example 2 Preparation of the soothing and repairing composition The composition prepared in this embodiment comprises, by weight percentage: 0.1% Centella asiatica extract, 2.0% Calendula officinalis extract, 1.0% meadowfoam seed oil, 5.0% Tween-20, 0.2% Carbomer 981, 0.05% hydroxyethyl cellulose, 0.3% allantoin, 0.2% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 7.5% 1,3-butanediol, and deionized water to 100%.

[0039] The preparation method of this embodiment is basically the same as that of Example 1, with the main difference being the dosage of each component and the adjustment of some process parameters. In the preparation of phase B, due to the increased amount of thickener, the swelling time was extended to 35 minutes to ensure sufficient hydration. In the emulsification step, because the oil phase content was increased to 1.0%, the homogenization time was correspondingly extended to 25 minutes, and the homogenization speed was adjusted to 11,000 rpm to obtain a finer and more stable emulsion system. The viscosity of the composition prepared in this embodiment is slightly higher than that of Example 1, approximately 8000-12000 mPa·s, making it suitable as a functional ingredient in face creams or lotions.

[0040] Example 3 Preparation of the soothing and repairing composition The composition prepared in this embodiment comprises, by weight percentage: 0.1% Centella asiatica extract, 3.0% Calendula officinalis extract, 0.55% meadowfoam seed oil, 3.0% Tween-20, 0.3% Carbomer 981, 0.03% hydroxyethyl cellulose, 0.2% allantoin, 0.3% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 10.0% 1,3-butanediol, and deionized water to 100%.

[0041] This embodiment uses the maximum addition amount of calendula extract, 3.0%, to fully utilize its anti-inflammatory and soothing effects. During the preparation process, the moisturizing properties of the system are significantly enhanced due to the use of 1,3-butanediol reaching 10.0%, and the stabilizing effect of the polyol on the active ingredients is also more pronounced. The main steps of the preparation method are the same as in Example 1, but the stirring time is appropriately extended to 20 minutes when mixing the calendula extract to ensure sufficient dispersion of the active ingredients. The composition prepared in this embodiment exhibits a deep yellow color, which is due to the high content of carotenoids in the calendula extract; this color is a sign of the presence of natural active ingredients.

[0042] Example 4 Preparation of the soothing and repairing composition The composition prepared in this embodiment comprises, by weight percentage: 0.55% Centella asiatica extract, 1.0% Calendula officinalis extract, 1.0% Meadowfoam seed oil, 5.0% Tween-20, 0.2% Carbomer 981, 0.05% Hydroxyethylcellulose, 0.3% Allantoin, 0.2% Triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 5.0% 1,3-butanediol, and deionized water to 100%.

[0043] This embodiment uses a moderate amount of Centella asiatica extract (0.55%), which represents the midpoint of the range of asiaticoside additions in this invention. At this addition level, the anti-inflammatory and repairing effects of asiaticoside are fully realized, and its synergistic effect with meadowfoam seed oil and calendula extract is more significant. The preparation method is basically the same as in Example 1. In the preparation of phase A, due to the increased amount of Centella asiatica extract, the dissolution time was appropriately extended to 40 minutes, and the stirring speed was appropriately increased to 400 rpm to accelerate the dissolution process.

[0044] Example 5 Preparation of the Soothing and Repairing Composition The composition prepared in this embodiment comprises, by weight percentage: 0.55% Centella asiatica extract, 2.0% Calendula officinalis extract, 0.55% meadowfoam seed oil, 3.0% Tween-20, 0.3% Carbomer 981, 0.03% hydroxyethyl cellulose, 0.2% allantoin, 0.3% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 7.5% 1,3-butanediol, and deionized water to 100%.

[0045] This embodiment employs a moderate ratio of three core active ingredients to achieve an optimal balance between cost and efficacy. During the preparation process, particular attention was paid to the uniformity of mixing each phase. When adding phase A to phase B, a batch addition method was used: first, one-third of phase A was added, thoroughly stirred, and then the remaining portion was added. This avoids clumping of the thickener due to excessively high local concentrations. The composition prepared in this embodiment exhibits excellent overall performance, possessing both good soothing and repairing effects while maintaining a moderate cost, making it suitable as a functional ingredient in daily skincare products.

[0046] Example 6 Preparation of the Soothing and Repairing Composition The composition prepared in this embodiment comprises, by weight percentage: 0.55% Centella asiatica extract, 3.0% Calendula officinalis extract, 0.1% meadowfoam seed oil, 1.0% Tween-20, 0.1% Carbomer 981, 0.01% hydroxyethyl cellulose, 0.1% allantoin, 0.1% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 10.0% 1,3-butanediol, and deionized water to 100%.

[0047] This embodiment uses the highest concentration of calendula extract (3.0%) and a medium concentration of centella asiatica extract (0.55%), while the amount of meadowfoam seed oil is the lowest (0.1%). This formulation focuses on enhancing the efficacy of water-soluble active ingredients and is suitable for preparing a refreshing skincare product. The preparation method is the same as in the previous embodiment. Due to the low oil phase content, the homogenization time in the emulsification step can be appropriately shortened to 18 minutes. The composition prepared in this embodiment has a refreshing texture, a viscosity of approximately 5000-8000 mPa·s, and penetrates quickly after application without being greasy.

[0048] Example 7 Preparation of the Soothing and Repairing Composition The composition prepared in this embodiment comprises, by weight percentage: 1.0% Centella asiatica extract, 1.0% Calendula officinalis extract, 0.55% Meadowfoam seed oil, 3.0% Tween-20, 0.3% Carbomer 981, 0.03% Hydroxyethylcellulose, 0.2% Allantoin, 0.3% Triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 5.0% 1,3-butanediol, and deionized water to 100%.

[0049] This embodiment uses the highest concentration of Centella asiatica extract (1.0%) to fully utilize the core efficacy of asiaticoside. During the preparation of phase A, due to the maximum amount of Centella asiatica extract, more deionized water was needed for dissolution, and the stirring intensity was appropriately increased. The completely dissolved phase A exhibits a deep yellow color, indicating a high concentration of asiaticoside. In subsequent mixing and emulsification steps, careful observation of the viscosity changes is necessary; the amount of thickener can be fine-tuned if required to achieve the desired texture. The composition prepared in this embodiment exhibits the strongest anti-inflammatory and repairing effects, making it particularly suitable for intensive repair of sensitive or damaged skin.

[0050] Example 8 Preparation of the Soothing and Repairing Composition The composition prepared in this embodiment comprises, by weight percentage: 1.0% Centella asiatica extract, 2.0% Calendula officinalis extract, 0.1% meadowfoam seed oil, 1.0% Tween-20, 0.1% Carbomer 981, 0.01% hydroxyethyl cellulose, 0.1% allantoin, 0.1% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 7.5% 1,3-butanediol, and deionized water to 100%.

[0051] This embodiment uses the highest concentration of Centella asiatica extract (1.0%) and a medium concentration of Calendula officinalis extract (2.0%), emphasizing the synergistic effect of water-soluble active ingredients. The preparation method is similar to Example 7; in the A-phase preparation stage, it is necessary to ensure complete dissolution of the Centella asiatica extract to avoid subsequent precipitation. Due to the low content of meadowfoam seed oil, the emulsification process is relatively simple, but it is still necessary to ensure sufficient dispersion of oil droplets. The composition prepared in this embodiment combines high efficacy with a refreshing texture, making it suitable for oily or combination-sensitive skin.

[0052] Example 9 Preparation of the Soothing and Repairing Composition The composition prepared in this embodiment comprises, by weight percentage: 1.0% Centella asiatica extract, 3.0% Calendula officinalis extract, 1.0% meadowfoam seed oil, 5.0% Tween-20, 0.2% Carbomer 981, 0.05% hydroxyethyl cellulose, 0.3% allantoin, 0.2% triethanolamine, 0.5% p-hydroxyacetophenone, 0.5% 1,2-hexanediol, 10.0% 1,3-butanediol, and deionized water to 100%.

[0053] This embodiment employs the highest addition levels of all core active ingredients, representing the high-efficacy formulation of the composition of this invention. During the preparation process, each step requires precise control to ensure that such a high concentration of active ingredients remains stable within the system. Due to the high total solids content, the swelling time needs to be appropriately increased to 40 minutes during phase B preparation, and the homogenization time in the emulsification step is extended to 30 minutes, with the rotation speed increased to 12,000 rpm. The composition prepared in this embodiment exhibits a deep yellow color, a rich texture, and a viscosity of approximately 15,000-20,000 mPa·s, demonstrating excellent soothing and repairing effects, making it suitable as a core ingredient in high-end functional skincare products.

[0054] Comparative Example 1: Composition lacking Centella Asiatica extract The composition prepared in this comparative example does not contain Centella asiatica extract. The remaining components, by mass percentage, are: Calendula officinalis extract 3.0%, meadowfoam seed oil 1.0%, Tween-20 5.0%, Carbomer 981 0.2%, hydroxyethyl cellulose 0.05%, allantoin 0.3%, triethanolamine 0.2%, p-hydroxyacetophenone 0.5%, 1,2-hexanediol 0.5%, 1,3-butanediol 10.0%, and deionized water to 100%.

[0055] The preparation method is basically the same as in Example 9, but the preparation step of phase A is omitted. After directly preparing phase B, the subsequent steps are completed according to the same process flow. Due to the lack of asiaticoside, the core anti-inflammatory component, although this composition still has a certain soothing effect, its anti-inflammatory strength and repair ability are significantly weaker than those of the examples containing the complete active ingredient.

[0056] Comparative Example 2: Composition lacking calendula extract The composition prepared in this comparative example does not contain calendula extract. The remaining components, by mass percentage, are: Centella asiatica extract 1.0%, meadowfoam seed oil 1.0%, Tween-20 5.0%, carbomer 981 0.2%, hydroxyethyl cellulose 0.03%, allantoin 0.1%, triethanolamine 0.2%, p-hydroxyacetophenone 0.5%, 1,2-hexanediol 0.5%, 1,3-butanediol 10.0%, and deionized water to 100%.

[0057] The preparation was carried out according to the method of the embodiment, but calendula extract was not added in step three. Without calendula extract, the composition lost the synergistic antioxidant and anti-inflammatory effects of carotenoids and flavonoids, resulting in a decrease in overall efficacy.

[0058] Comparative Example 3: Composition lacking meadowfoam seed oil The composition prepared in this comparative example does not contain meadowfoam seed oil. The remaining components, by mass percentage, are: Centella asiatica extract 1.0%, Calendula officinalis extract 3.0%, Tween-20 5.0%, Carbomer 981 0.2%, Hydroxyethylcellulose 0.03%, Allantoin 0.1%, Triethanolamine 0.2%, p-hydroxyacetophenone 0.5%, 1,2-hexanediol 0.5%, 1,3-butanediol 10.0%, and deionized water to 100%.

[0059] The oil phase emulsification step was omitted during preparation, and the remaining steps were the same as in the example. This composition is in an aqueous solution or gel state, lacking the fat-soluble antioxidant components and barrier repair function provided by meadowfoam seed oil, and is significantly insufficient in terms of long-lasting moisturizing and deep repair.

[0060] Comparative Example 4: Composition containing only meadowfoam seed oil This comparative example contains only meadowfoam seed oil as the active ingredient, and does not contain centella asiatica extract or calendula extract. The remaining components, by weight percentage, are: meadowfoam seed oil 1.0%, Tween-20 5.0%, carbomer 981 0.2%, hydroxyethyl cellulose 0.03%, allantoin 0.1%, triethanolamine 0.2%, p-hydroxyacetophenone 0.5%, 1,2-hexanediol 0.5%, 1,3-butanediol 10.0%, and deionized water to make up to 100%.

[0061] The preparation method is simplified; phase B is prepared directly followed by oil phase emulsification. While this composition possesses certain moisturizing and antioxidant properties, its anti-inflammatory and repair-promoting effects are extremely limited, failing to meet the requirements for soothing and repairing efficacy.

[0062] Comparative Example 5: Compositions containing only calendula extract This comparative example contains only one active ingredient, Calendula officinalis extract, and does not contain Centella asiatica extract or meadowfoam seed oil. The remaining components, by weight percentage, are: Calendula officinalis extract 3.0%, Tween-20 5.0%, Carbomer 981 0.2%, Hydroxyethylcellulose 0.03%, Allantoin 0.1%, Triethanolamine 0.2%, p-Hydroxyacetophenone 0.5%, 1,2-Hexanediol 0.5%, 1,3-Butanediol 10.0%, and deionized water to make up to 100%.

[0063] The preparation process is relatively simple as it does not require oil-phase emulsification. Although this composition retains the soothing and anti-inflammatory properties of calendula, it lacks the deep anti-inflammatory effects of asiaticoside and the barrier-repairing function of meadowfoam seed oil, resulting in a significant reduction in its overall effectiveness.

[0064] Comparative Example 6: Compositions containing only Centella asiatica extract This comparative example contains only one active ingredient, Centella Asiatica extract, and does not contain Calendula officinalis extract or meadowfoam seed oil. The remaining components, by weight percentage, are: Centella Asiatica extract 1.0%, Tween-20 5.0%, Carbomer 981 0.2%, Hydroxyethylcellulose 0.03%, Allantoin 0.1%, Triethanolamine 0.2%, p-Hydroxyacetophenone 0.5%, 1,2-Hexanediol 0.5%, 1,3-Butanediol 10.0%, and deionized water to make up to 100%.

[0065] The preparation method is similar to that in the examples, but without the emulsification step. Although asiaticoside has good anti-inflammatory and repairing effects, it lacks the synergistic effect of other ingredients when used alone and cannot provide comprehensive skin care benefits.

[0066] Please refer to Figure 1 and Figure 2 To comprehensively evaluate the soothing and repairing efficacy of the compositions of the present invention, systematic performance tests were conducted on the above-mentioned examples and comparative examples. The tests included hyaluronidase inhibition rate testing, cell proliferation and wound healing capacity testing, and safety evaluation.

[0067] Test 1: Hyaluronidase Inhibition Rate Test Hyaluronidase is a specific cleavage enzyme of hyaluronic acid, playing a crucial role in skin inflammation and allergic reactions. This enzyme degrades hyaluronic acid in the extracellular matrix, increases vascular permeability, and promotes the release and diffusion of inflammatory mediators. Hyaluronidase is strongly correlated with the release of inflammatory factors such as histamine from mast cells; therefore, inhibiting hyaluronidase activity is an important indicator for evaluating soothing and anti-inflammatory efficacy. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance.

[0068] This test, following laboratory methods, employed an in vitro enzyme inhibition method to assess the soothing efficacy of the samples. This method quantitatively evaluates the anti-inflammatory and soothing abilities by measuring the degree to which the samples inhibit the degradation of hyaluronidase-catalyzed hyaluronic acid. The test included positive controls, negative controls, and a sample group, and differences between groups were compared through statistical analysis.

[0069] The main instruments used in the test included a BSA224S analytical balance for accurately weighing reagents and samples with an accuracy of 0.1 mg. An L6s UV spectrophotometer was used to determine the absorbance of the reaction products, with a wavelength range of 190-1100 nm and a wavelength accuracy of ±0.5 nm.

[0070] The main reagents used in the test included hyaluronidase, a biochemical reagent grade derived from bovine testis, with an enzyme activity of 300-600 units / mg; sodium hyaluronate, a biochemical reagent grade with a molecular weight of approximately 1 million Daltons and a purity greater than 95%; dipotassium glycyrrhizate, with a purity greater than 98%, diluted with pure water to a mass fraction of 3%; and pure water as the negative control.

[0071] The specific steps of the testing method are as follows: For sample preparation, the compositions prepared in Examples 1 to 9 and Comparative Examples 1 to 6 were used directly as test samples at a concentration of 10%. The positive control, dipotassium glycyrrhizate, was diluted with pure water to a mass fraction of 3%. The negative control was pure water.

[0072] The reaction system was set up with four groups: sample group, sample background group, solvent group, and solvent background group, with three replicates in each group. The sample group contained sample solution, hyaluronidase solution, and sodium hyaluronate substrate solution. The sample background group contained sample solution and sodium hyaluronate substrate solution, but no enzyme solution, used to subtract interference from the sample's own absorbance. The solvent group contained pure water, hyaluronidase solution, and sodium hyaluronate substrate solution, representing normal enzyme activity. The solvent background group contained pure water and sodium hyaluronate substrate solution, but no enzyme solution, used to subtract background absorbance from the substrate.

[0073] After adding the corresponding reagent solutions to each group, mix thoroughly and incubate in a 37°C water bath for 45 minutes to allow the enzymatic reaction to proceed fully. After the reaction is complete, add the colorimetric reagent and incubate at room temperature for 30 minutes to allow the colorimetric reaction to develop. After the colorimetric reaction is complete, use a UV spectrophotometer to measure the absorbance of each reaction system at a wavelength of 528 nm.

[0074] The hyaluronidase inhibition rate is calculated using the following formula: Hyaluronidase inhibition rate (%) = (1- )×100 In the formula: A—is the absorbance of the reaction solution without the sample; B—is the absorbance of the reaction solution without the sample and enzyme. C—Absorbance of the reaction solution containing the sample and enzyme; D—Absorbance of the reaction solution containing the sample and without the enzyme.

[0075] Statistical analysis was performed using SPSS software. Independent samples t-tests were used to compare hyaluronidase inhibition rates among the examples, comparative examples, positive controls, and negative controls. All statistical analyses were two-tailed tests, with a significance level set at α = 0.05. A p-value greater than 0.05 indicated no significant difference between the two groups; a p-value less than 0.05 indicated a significant difference between the two groups.

[0076] The test results are shown in Table 1: Table 1 Results of Hyaluronidase Inhibition Rate Test

[0077] As shown in Table 1, the positive control, dipotassium glycyrrhizate, exhibited a hyaluronidase inhibition rate of 69.151%, significantly higher than 50%, indicating that the reaction system was effective and reliable. The negative control, pure water, showed a negative inhibition rate, which is normal because pure water does not have the ability to inhibit enzyme activity; the slight fluctuation in its value is due to measurement error.

[0078] The hyaluronidase inhibition rates of Examples 1 to 9 ranged from 54.217% to 62.277%, all significantly higher than the negative control, with P values ​​less than 0.05, indicating statistically significant differences. These data fully demonstrate that the composition containing all three core active ingredients has excellent soothing efficacy. Notably, Example 9, using the highest addition amount of all active ingredients, achieved an inhibition rate of 62.277%, the best among all examples, approaching the level of the positive control.

[0079] The hyaluronidase inhibition rates of Comparative Examples 1 to 6 ranged from only 26.785% to 43.522%, significantly lower than those of the Example Group. Comparative Example 4, containing only meadowfoam seed oil, had the lowest inhibition rate at only 26.785%. Comparative Example 5, containing only calendula extract, had an inhibition rate of 30.652%. Comparative Example 6, containing only centella asiatica extract, had an inhibition rate of 36.876%, which, although relatively high among the comparative examples, was still far lower than that of the Examples containing complete components. Comparative Examples 1, 2, and 3, each lacking a core component, had inhibition rates ranging from 35.638% to 43.522%, which, while better than Comparative Examples 4 to 6 containing only a single component, were still significantly lower than those of the Example Group.

[0080] Comparative data from the examples and comparative studies clearly demonstrate that the synergistic effect of the three core active ingredients is crucial for the soothing efficacy. The absence of any one ingredient leads to a significant decrease in overall efficacy, and when all three are present, their inhibitory rates are not simply additive, but exhibit a synergistic effect. This result can be explained at the molecular level as follows: asiaticoside exerts its effect by inhibiting inflammatory signaling pathways; flavonoids in calendula extract scavenge free radicals and stabilize cell membranes; and tocopherols in meadowfoam seed oil provide long-lasting antioxidant protection and repair damaged barriers. These three ingredients work together at different levels to address the inflammatory process, thus achieving a synergistic effect far exceeding that of any single ingredient.

[0081] Tests for two-cell proliferation and wound healing capabilities The skin repair process after injury involves multiple phases, including the inflammatory phase, the proliferative phase, and the remodeling phase. During the proliferative phase, the proliferation and migration of fibroblasts are crucial for wound healing. NIH / 3T3 cells, a mouse embryonic fibroblast cell line, are widely used to evaluate the effects of substances on cell proliferation and wound healing. This assay comprehensively evaluates the repair efficacy of the composition by detecting the effect of the sample on the proliferation rate of NIH / 3T3 cells and their ability to migrate into simulated wound areas.

[0082] Cell proliferation rate was detected using the MTT assay. NIH / 3T3 cells were seeded in 96-well plates at 5000 cells per well and cultured for 24 hours until cell attachment. The culture medium was then replaced with different concentrations of the test sample. The sample concentration was set at 1%, simulating the amount added in practical applications. A blank control group was set up, containing only complete culture medium without the sample. After culturing for another 48 hours, MTT solution was added to each well, and the plates were incubated at 37°C for 4 hours to allow mitochondrial dehydrogenases in the living cells to reduce MTT to purple formazan crystals. The culture medium was carefully aspirated, and dimethyl sulfoxide was added to each well to dissolve the formazan crystals. The plates were shaken for 10 minutes to ensure complete dissolution. The absorbance at 570 nm was measured using a microplate reader. Cell proliferation rate was calculated as the absorbance of the sample group divided by the absorbance of the control group, then multiplied by 100%.

[0083] Wound healing capacity was assessed using a scratch assay. NIH / 3T3 cells were seeded in 6-well plates and cultured until confluence exceeded 90%, forming a dense monolayer. A straight scratch approximately 1 mm wide was made on the cell monolayer using a 200 μL pipette tip to simulate a wound. The cells were gently rinsed three times with phosphate-buffered saline (PFS) to remove cell debris detached during the scratching process. Serum-free culture medium containing the test sample (1% concentration) was added. The initial state of the scratch was photographed under an inverted microscope, and the observation location was marked. The cells were then cultured at 37°C with 5% CO2 for 24 hours. After culture, the same location was photographed again to record cell migration in the scratched area. Image analysis software was used to calculate the number of cells entering the scratched area; this number reflects cell migration capacity and indirectly characterizes the wound healing rate.

[0084] The test results are shown in Table 2: Table 2 Results of cell proliferation and wound healing ability tests

[0085] Table 2 clearly demonstrates the significant differences between the examples and the comparative examples in promoting cell proliferation and wound healing. The NIH / 3T3 cell proliferation rates in Examples 1 through 9 ranged from 148.5% to 162.3%, all significantly higher than the 125.8% to 138.8% in the comparative examples. Example 9 achieved a cell proliferation rate of 162.3%, indicating that the formulation significantly promoted fibroblast proliferation, which is crucial for the repair of skin damage. With increasing Centella asiatica extract content, from 0.1% in Examples 1, 2, and 3 to 1.0% in Examples 7, 8, and 9, the cell proliferation rate showed a gradual upward trend, consistent with the known cell proliferation-promoting function of asiaticoside.

[0086] Regarding wound healing ability, the number of cells entering the wound area in the Example group ranged from 3874 to 5523 after 24 hours, while the number in the Comparative Example group was only 2578 to 2872. Example 9 showed the highest number of cell migrations, reaching 5523, approximately 2.1 times that of Comparative Example 4. This significant difference indicates that the composition containing the complete active ingredient not only promotes cell proliferation but also enhances cell migration ability, thereby accelerating the wound closure process.

[0087] In the comparative examples, Comparative Example 6, containing only Centella asiatica extract, showed a cell proliferation rate of 135.3% and a number of migrating cells of 2791, which was relatively good among the comparative examples, reflecting the core role of asiaticoside in promoting repair. However, even so, its effect was still far inferior to the examples containing all three ingredients. Comparative Example 1 lacked Centella asiatica extract, Comparative Example 2 lacked Calendula officinalis extract, and Comparative Example 3 lacked meadowfoam seed oil; their repair capabilities were all significantly weaker than the examples, further confirming the importance of the synergistic effect of the three ingredients.

[0088] From a mechanistic perspective, asiaticoside can promote the synthesis of collagen and glycosaminoglycans by fibroblasts, which are essential components of the extracellular matrix and indispensable for cell proliferation and tissue repair. The flavonoids in calendula extract have antioxidant effects, protecting cells from oxidative stress damage and maintaining normal cell proliferation and differentiation. The unsaturated fatty acids and phytosterols in meadowfoam seed oil can regulate cell membrane fluidity and promote growth factor receptor signal transduction, thereby enhancing the cell's responsiveness to growth stimuli. These three components work synergistically in multiple stages, including extracellular matrix synthesis, antioxidant protection, and cell signal regulation, jointly promoting cell proliferation and wound healing.

[0089] Test 3 Security Evaluation The safety of cosmetic ingredients is a prerequisite for their widespread application, especially for products claiming soothing and repairing effects and targeting sensitive skin, where safety requirements are even more stringent. This test uses the 2015 "Cosmetic Safety Technical Specifications" as a reference standard to conduct a patch test on the soothing and repairing composition prepared in this invention to evaluate its skin irritation and sensitization.

[0090] The trial recruited 15 healthy volunteers aged 16 to 65 years, regardless of gender, who were required to have no recent history of skin disease, no known cosmetic allergies, and intact, unbroken skin on their backs or the flexor surfaces of their forearms. The 15 participants were randomly divided into three groups of five, and the compositions prepared in Examples 1, 5, and 9 were tested respectively. These three examples were chosen because they represent low, medium, and high levels of the active ingredient, respectively.

[0091] The specific procedure for the test is as follows: Place the test composition into a dedicated plaster applicator, using 0.020 to 0.025 grams per applicator, ensuring the sample evenly covers the entire test area. Secure the plaster applicator containing the test compound to the skin on either side of the spine on the subject's back or on the inner side of the flexor forearm using non-irritating adhesive tape. Avoid bony prominences and choose a relatively smooth area of ​​skin. Gently press the plaster applicator with your palm to ensure it adheres evenly to the skin surface, guaranteeing full contact between the test compound and the skin.

[0092] The patch applicator is applied continuously for 24 hours. During this period, subjects are instructed to avoid strenuous exercise and bathing, and to keep the application site dry. After 24 hours, the patch applicator and adhesive tape are carefully removed. The test site is gently wiped with a cotton swab dipped in saline solution to remove any residual test substance. Thirty minutes after removing the patch applicator, once the skin indentation has disappeared, a professional dermatologist observes the skin reaction at the test site under natural light and records the results. If the initial observation result is negative, repeat observations are required at 24 and 48 hours after the patch test to rule out the possibility of delayed-type allergic reactions.

[0093] The evaluation criteria for skin reaction are divided into 5 levels: Level 0 is a negative reaction with no changes in the skin; Level 1 is a questionable reaction with only weak erythema and indistinct borders; Level 2 is a weak positive reaction with obvious erythema, mild infiltration or edema, and a small number of papules; Level 3 is a strong positive reaction with obvious erythema, significant infiltration and edema, and a large number of papules, and the reaction may extend beyond the tested area; Level 4 is an extremely strong positive reaction with obvious erythema, severe infiltration and edema, confluent herpes, and the reaction significantly extends beyond the tested area.

[0094] The test results showed that all 15 subjects had a grade 0 negative skin reaction during observations at 30 minutes, 24 hours, and 48 hours after the removal of the spot treatment device. No abnormal changes were observed in skin color, texture, moisture, or other indicators at the test site; no erythema, edema, papules, or any discomfort were observed. Subjectively, all subjects reported no itching, stinging, burning, or other discomfort.

[0095] This result fully demonstrates that the soothing and repairing composition provided by this invention is gentle and non-irritating to human skin, will not cause allergic reactions, and is safe and reliable to use. Even in Example 9, which uses the highest content of active ingredients, its safety remains excellent, indicating that the formulation design of this invention is reasonable and the addition amount of each ingredient is controlled within a safe range. The composition is particularly suitable for people with sensitive skin and can be used safely for a long time, providing an ideal choice of functional raw materials for the daily care and damage repair of sensitive skin.

[0096] Taking into account factors such as soothing and repairing efficacy, safety, stability, and cost, Example 5 is the optimal embodiment of the present invention. This embodiment uses three core active ingredients in moderate amounts: Centella asiatica extract 0.55%, Calendula officinalis extract 2.0%, and meadowfoam seed oil 0.55%, achieving effective cost control while ensuring excellent efficacy.

[0097] Example 5 showed a hyaluronidase inhibition rate of 54.653%, an NIH / 3T3 cell proliferation rate of 155.6%, and 4731 cells entering the wound after 24 hours. These indicators were significantly better than the comparative example, fully demonstrating its excellent soothing and repairing effects. Meanwhile, the raw material cost of this formulation was reduced by approximately 30% compared to Examples 7 to 9, making it more suitable for large-scale production and market promotion. Regarding stability, after 12 months of storage at room temperature, the composition prepared in Example 5 showed no significant changes in any of its performance indicators. The pH value remained stable between 6.5 and 7.0, the viscosity remained within the range of 10000 to 12000 mPa·s, and there were no abnormalities in appearance, odor, or color.

[0098] The composition prepared in Example 5 has a fine texture, good spreadability, and a refreshing, non-greasy feel on the skin. It is suitable for addition to washing and care products to provide soothing effects, and also suitable as a core active ingredient in skincare products. In practical application testing, this composition was added to a face cream base at a dosage of 5%. After 30 volunteers with sensitive skin used it continuously for 4 weeks, symptoms such as skin erythema and itching were significantly reduced, skin barrier function was significantly improved, transepidermal water loss rate was reduced by approximately 35%, and the subject satisfaction rate reached 93%.

[0099] The reason why the composition of the present invention can achieve a significant synergistic effect can be explained in detail from the perspective of molecular level and mechanism of action as follows: In terms of anti-inflammatory mechanisms, asiaticoside, as a triterpenoid saponin, contains multiple hydroxyl groups and glycosidic bonds in its molecular structure. This unique structure allows it to bind to Toll-like receptor 2 on the cell membrane, blocking the receptor-mediated inflammatory signaling cascade. Specifically, asiaticoside inhibits downstream signaling molecules following Toll-like receptor 2 activation, including myeloid differentiation factor 88 and interleukin-1 receptor-associated kinase, thereby blocking the translocation of nuclear factor κB from the cytoplasm to the nucleus. Nuclear factor κB is a key transcription factor regulating the expression of various pro-inflammatory cytokine genes. Inhibition of its activity significantly reduces the synthesis and secretion of pro-inflammatory factors such as interleukin-1β, interleukin-6, and tumor necrosis factor-α, effectively controlling the inflammatory response.

[0100] Flavonoids in calendula extract, such as quercetin and kaempferol, possess a conjugated double bond system that can provide reactive hydrogen atoms to quench free radicals. During inflammation, the large amounts of reactive oxygen species and free radicals attack cell membrane lipids, triggering lipid peroxidation and further exacerbating inflammatory damage. Flavonoids break the vicious cycle of inflammation by scavenging these harmful free radicals. Simultaneously, triterpenoid saponins can stabilize mast cell membranes and reduce the release of inflammatory mediators such as histamine, thus exerting an inhibitory effect in the early stages of the inflammatory response.

[0101] Meadowfoam seed oil is rich in α-tocopherol, a lipid-soluble antioxidant that can integrate into the phospholipid bilayer of the cell membrane. Within the membrane structure, it scavenge lipid peroxidation free radicals, protecting the integrity and fluidity of the cell membrane. Cell membrane stability is crucial for maintaining normal cell function, especially under inflammatory conditions where the cell membrane is susceptible to oxidative damage. The protective effect of α-tocopherol extends beyond free radical scavenging; it can also regenerate other antioxidants, such as ascorbic acid, forming an antioxidant network that provides long-lasting protection.

[0102] The synergistic effect of the three components in anti-inflammatory and antioxidant aspects is manifested in the following ways: asiaticoside blocks the initiation and amplification of the inflammatory response at the signaling pathway level; calendula extract reduces the release of inflammatory factors at the inflammatory mediator level; and meadowfoam seed oil maintains the integrity of cell structure and function at the cell membrane protection level. These three components act on different stages of the inflammatory process, forming a three-dimensional anti-inflammatory protection system. This is the fundamental reason why the hyaluronidase inhibition rate of the example is much higher than that of the comparative examples with single components or incomplete components.

[0103] In terms of promoting repair mechanisms, asiaticoside can stimulate fibroblasts to synthesize type I and type III collagen. Collagen is the main supporting component of skin structure, and the deposition of newly formed collagen is crucial for restoring the mechanical strength of the skin during wound healing. asiaticoside activates the transforming growth factor β signaling pathway, upregulates collagen gene expression, promotes the synthesis and hydroxylation modification of procollagen peptide chains, and ultimately increases the formation of mature collagen fibers. Simultaneously, asiaticoside can also promote the synthesis of glycosaminoglycans. These macromolecular polysaccharides constitute the matrix components of the extracellular matrix, providing a scaffold for cell migration and creating a favorable microenvironment for tissue repair.

[0104] The polysaccharides in calendula extract have immunomodulatory effects, moderately activating macrophages and promoting the secretion of growth factors such as epidermal growth factor and vascular endothelial growth factor. These growth factors play an important role in stimulating epidermal cell proliferation, angiogenesis, and granulation tissue formation. Simultaneously, the antioxidant components of calendula extract protect newly formed cells from oxidative stress damage, improve cell survival rate, and accelerate tissue repair.

[0105] The unsaturated fatty acids in meadowfoam seed oil, particularly erucic acid and oleic acid, can integrate into the cell membrane, regulating membrane lipid composition and influencing membrane protein function. The activity of certain growth factor receptors and ion channels depends on cell membrane fluidity and the lipid microenvironment. Meadowfoam seed oil indirectly promotes cell proliferation and differentiation by optimizing the membrane lipid environment and enhancing cell sensitivity to growth signals. Furthermore, phytosterols have hormone-like effects, regulating gene expression, promoting protein synthesis, and supporting cell growth and repair processes.

[0106] The three components exhibit synergistic effects in promoting repair: asiaticoside provides the material basis for tissue reconstruction at the matrix synthesis level; calendula extract stimulates cell proliferation and angiogenesis at the growth factor regulation level; and meadowfoam seed oil enhances the cell's responsiveness to repair signals at the cell membrane optimization level. This multi-level, multi-target synergistic effect enables the composition of this invention to demonstrate outstanding performance in promoting cell proliferation and wound healing.

[0107] It is worth emphasizing that the synergistic effect of the composition of this invention is not a simple additive effect, but rather based on the complementarity of the three active ingredients in terms of molecular structure, mechanism of action, and target localization. The triterpenoid saponin structure of asiaticoside, the flavonoid and polysaccharide structure of calendula extract, and the fatty acid and sterol structure of meadowfoam seed oil endow them with different functional properties, namely water-soluble signal modulation, water-soluble antioxidant, and lipid-soluble membrane protection. The three work together to act on various aspects of skin inflammation and repair, forming a complete efficacy chain from signaling pathways to inflammatory mediators, from free radical scavenging to membrane structure protection, and from matrix synthesis to cell proliferation. This is the scientific basis for the remarkable efficacy of this invention with relatively low concentrations of active ingredients.

[0108] Furthermore, the dosage form design of the composition of this invention also plays a crucial supporting role in its efficacy. The emulsion system allows the fat-soluble meadowfoam seed oil and the water-soluble asiaticoside and calendula extract to coexist stably. Through the interfacial film formed by the emulsifier, the oil and water phase components can fully contact and interact. The three-dimensional network structure formed by the thickener not only stabilizes the emulsion but also provides a sustained-release effect for the active ingredients, enabling them to act continuously on the skin and prolonging the duration of efficacy. The moisturizing effect of the polyol system maintains the skin's hydration state, creating a favorable skin microenvironment for the penetration and efficacy of the active ingredients. This perfect combination of dosage form and ingredients ensures that the composition of this invention can fully exert its designed efficacy in practical applications.

[0109] In summary, the soothing and repairing composition provided by this invention achieves a significant synergistic effect through the scientific combination of three core active ingredients: asiaticoside, meadowfoam seed oil, and calendula extract. Extensive experimental data fully demonstrate that this composition possesses excellent soothing, anti-inflammatory, and repair-promoting efficacy, while exhibiting superior safety, good stability, and reasonable cost, making it suitable for large-scale industrial production and widespread application. This invention provides the cosmetics industry with a highly efficient, safe, and economical functional raw material, possessing significant practical value and broad application prospects for meeting the skincare needs of people with sensitive skin and promoting the development of soothing and repairing products.

[0110] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. For those skilled in the art, the present invention can be modified and varied in various ways. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A composition having a soothing and repairing effect, characterized in that, It consists of the following components by mass percentage: Hydroxyacinthoside 0.1%-1.0%, meadowfoam seed oil 0.1%-1.0%, calendula extract 1.0%-3.0%, allantoin 0.1%-0.3%, hydroxyethyl cellulose 0.01%-0.05%, carbomer 981 0.1%-0.3%, Tween-20 surfactant 1%-5%, triethanolamine 0.1%-0.3%, p-hydroxyacetophenone 0.5%, 1,2-hexanediol 0.5%, 1,3-butanediol 5.0%-10%, deionized water to 100%.

2. The composition with soothing and repairing effects according to claim 1, characterized in that, The content of asiaticoside is 0.55%, the content of meadowfoam seed oil is 0.55%, and the content of calendula extract is 2.0%.

3. The composition with soothing and repairing effects according to claim 1, characterized in that, The asiaticoside is derived from Centella asiatica extract, and the content of asiaticoside in the Centella asiatica extract is 90%.

4. The composition with soothing and repairing effects according to claim 1, characterized in that, The main components of the meadowfoam seed oil include long-chain fatty acids, natural tocopherols, and phytosterols, while the main components of the calendula extract include carotenoids, flavonoids, triterpenoid saponins, volatile oils, phytosterols, and polysaccharides.

5. The composition with soothing and repairing effects according to claim 1, characterized in that, The composition is in the form of an emulsion, and the composition has an inhibition rate of 54%-63% against hyaluronidase. Under the action of the composition, the proliferation rate of NIH / 3T3 cells is 148%-163%.

6. A method for preparing the composition with soothing and repairing effects according to any one of claims 1 to 5, characterized in that, Includes the following steps: Step 1: Dissolve the Centella asiatica extract in deionized water at room temperature to obtain phase A. The content of asiaticoside in the Centella asiatica extract is 90%. Step 2: Allantoin, hydroxyethyl cellulose, and carbomer 981 are added to hot water and allowed to swell completely to obtain phase B; Step 3: Mix phase A and phase B evenly, then add Tween-20 surfactant, triethanolamine, calendula extract and the remaining deionized water in sequence, and stir to mix evenly to obtain phase C; Step 4: Add p-hydroxyacetophenone to 1,2-hexanediol and heat and stir at 60°C to 90°C until p-hydroxyacetophenone is completely dissolved. After cooling, add 1,3-butanediol and mix evenly. Add the resulting mixture to phase C and stir evenly to obtain phase D. Step 5: Place the D phase in a homogenizer, slowly add meadowfoam seed oil during the homogenization and dispersion process, and continue homogenization and emulsification until a stable emulsion system is formed to obtain the composition with soothing and repairing effects.

7. The preparation method according to claim 6, characterized in that, The temperature of the hot water in step two is 70°C to 90°C, and the swelling time is 20 minutes to 40 minutes.

8. The preparation method according to claim 6, characterized in that, In step five, the homogenizer rotates at a speed of 8,000 to 12,000 rpm, the homogenization and emulsification time is 15 to 30 minutes, and the meadowfoam seed oil is added at a rate of 5% to 10% of the total amount per minute.

9. The use of the composition with soothing and repairing effects according to any one of claims 1 to 5 in the preparation of cosmetics, characterized in that, The cosmetics are personal care products or skin care products. The personal care products include shampoo, conditioner, and shower gel, while the skin care products include face cream, lotion, serum, and face mask.

10. The application according to claim 9, characterized in that, The composition is added to the cosmetic at a rate of 1% to 10% of the total mass of the cosmetic. The composition exerts a soothing and repairing effect by inhibiting the skin inflammatory response caused by the pro-inflammatory cytokine IL-1β. The cosmetic is suitable for soothing and repairing care of sensitive skin.

Images