Bird's nest acid mild acid brushing composition and application thereof

Abstract

The invention discloses a sialic acid mild brushing acid composition and application thereof, and relates to the technical field of cosmetics. The sialic acid mild brushing acid composition comprises sialic acid and a solvent, the mass concentration of the sialic acid is 2%-15%, and the solvent is selected from at least one of 1, 3-propylene glycol, butanediol, dipropylene glycol and water. The sialic acid is creatively used as one of the mild brushing acid components, can soften old and waste cutin mildly and promote cutin exfoliation and permeation and absorption of the sialic acid, so that the sialic acid can fully play a skin protection role, has an excellent mild cutin exfoliation effect and has the effects of improving hyperpigmentation and coarse pores; and a dynamic balance skin care strategy of breaking while standing and breaking while standing can be realized. The prepared cosmetics have better moisture retention and safety, repair barriers through moisture retention, improve pigmentation through oxidation resistance, stimulate collagen to remodel a supporting structure to improve coarse pores, and finally consolidate the health state of the skin through immunoregulation.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more specifically, to a mild exfoliating composition containing sialic acid and its application. Background Technology

[0002] In recent years, with the increasing demand for skin care, chemical peels have gained widespread attention as an effective skin management method. "Acid peeling," as an important component of chemical peeling systems, primarily uses substances containing specific chemical components to exfoliate the stratum corneum and promote skin regeneration. Currently, common acid peeling ingredients include salicylic acid, fruit acids (such as citric acid, mandelic acid, and malic acid), and retinoic acid.

[0003] Chemical peeling systems face the following challenges: (1) Destructive metabolism of single acids - Single-component mechanisms, mainly fruit acids and salicylic acid, achieve skin peeling by forcibly exfoliating the stratum corneum and dissolving sebum, but at the same time, they are more irritating to the skin, and are also prone to causing stinging, dryness, itching, redness, and even allergies and peeling. (2) Lack of repair system - Existing acid peeling ingredients mostly adopt the strategy of "only destroying without building" or "destroying first and then building"; for people with sensitive skin and thin stratum corneum, using single-component acid peeling in (1) may result in the skin barrier being damaged and not being soothed and repaired in time, resulting in problems such as redness, stinging, and peeling.

[0004] How to achieve gentle exfoliation and immediate repair of dead skin cells, and improve skin condition by improving pigmentation and pore management, thus realizing skin resurfacing care, is one of the technical problems that urgently need to be solved in this field.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] The purpose of this invention is to provide a mild acid-exfoliating composition for sialic acid and its application, in order to solve the above-mentioned technical problems.

[0007] This invention is implemented as follows: In a first aspect, embodiments of the present invention provide a mild sialic acid exfoliating composition comprising sialic acid and a solvent, wherein the mass concentration of sialic acid is 2%-15% and the solvent is selected from at least one of 1,3-propanediol, butanediol, dipropylene glycol and water.

[0008] Secondly, embodiments of the present invention provide a toner comprising component A and component B, with the remainder being water, wherein component B comprises the sialic acid mild exfoliating composition as described in the foregoing embodiments. By weight percentage, component A includes 3%-10% humectant, 0.5%-1.5% preservative, and 0.3%-0.8% thickener; component B includes 0.01%-20% composition, 0.2%-1.5% pH adjuster, and 0.5%-1.5% preservative.

[0009] Thirdly, embodiments of the present invention provide a method for preparing a toner as described in the foregoing embodiments, comprising the following preparation steps: Mix component A in the specified proportions, heat to 80℃-85℃, stir until completely dissolved, add premixed component B, and stir evenly to obtain toner.

[0010] Fourthly, embodiments of the present invention provide a cosmetic product comprising a mild acid-exfoliating composition of sialic acid as described in the foregoing embodiments.

[0011] Fifthly, embodiments of the present invention provide the application of a mild exfoliating composition of sialic acid as described in the foregoing embodiments, or a toner as described in the foregoing embodiments, or a toner prepared by the preparation method as described in the foregoing embodiments, or a cosmetic as described in the foregoing embodiments, in improving skin condition.

[0012] The present invention has the following beneficial effects: This invention creatively uses sialic acid as one of the gentle exfoliating ingredients, which can gently soften dead skin cells, promote exfoliation and the penetration and absorption of sialic acid, so that sialic acid can fully play its role in skin protection, with excellent gentle exfoliation effect, and can improve pigmentation and enlarged pores; it is conducive to achieving a dynamic balance skin care strategy of "breaking and building simultaneously".

[0013] Cosmetics made from a gentle exfoliating composition of sialic acid have better moisturizing properties and safety. After gently exfoliating dead skin cells, they simultaneously moisturize and repair the skin barrier, improve pigmentation through anti-oxidation, stimulate collagen remodeling to improve enlarged pores, and ultimately consolidate the skin's health through immune regulation. Attached Figure Description

[0014] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 The toner prepared using Example 1; Figure 2 The results are valid examples of the pigmentation test; Figure 3The results are valid examples of the pore size test. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0017] The outermost layer of skin, the stratum corneum, is composed of tightly packed dead keratinocytes, connected by structures called desmosomes, which act like "glue." The stability of desmosomes largely depends on calcium ions (Ca). 2+ The concentration of ) is crucial. The natural shedding process of the stratum corneum depends on the activity of a series of proteolytic enzymes, such as desmosome degrading enzymes.

[0018] Based on scientific research, the research team of this invention discovered that sialic acid is an acidic component with multiple skin-protective effects and possesses an α-hydroxy acid structure. This makes sialic acid an effective calcium ion chelating agent. When sialic acid penetrates the stratum corneum, it "captures" and reduces calcium levels in the environment surrounding desmosomes. 2+ Concentration, Ca 2+ The loss of sialic acid destabilizes the cadherin structure that makes up desmosomes, significantly weakening or even destroying their connectivity. This directly leads to decreased cohesion between keratinocytes, causing tightly connected dead skin cells to loosen and detach more easily from the skin surface. Sialic acid, a weak organic acid, further lowers the pH of the skin when applied to the surface. This acidified environment increases the activity of proteolytic enzymes, accelerating the breakdown of desmosomes between cells.

[0019] Sialic acid, scientifically known as N-acetylneuraminic acid, although its name contains the word "acid," is a non-carbon monosaccharide containing α-hydroxyl groups in biochemical classification. Its structure contains multiple bioactive groups that other skin care ingredients do not have, which theoretically endow it with multiple effects of exfoliation, soothing, and repair: (1) Carboxyl group (-COOH): The carboxyl group at the end of the molecule makes sialic acid acidic and hydrophilic, which can dissolve the protein bonds between keratinocytes, making it easier to exfoliate. (2) The presence of acetylamino group (-NHCOCH3) improves the stability and permeability of sialic acid, promotes the normalization of skin hydration, normalizes the metabolism of glycoproteins on the surface of keratinocytes, and maintains the normal metabolic function of surface keratinocytes. (3) The presence of glycerol side chain (-CH(OH)CH2OH) increases lipophilicity, which makes sialic acid have good skin affinity. Even after rinsing, it can form a film and be adsorbed on the skin, exerting the effects of enhanced penetration and continuous hydration, moisturizing and softening.

[0020] Sialic acid is the core active ingredient in bird's nest. The national standard GH / T 1092-2014, "Quality Grades of Bird's Nest," stipulates that the sialic acid content in premium-grade bird's nest must be ≥10%. As an endogenous bioactive substance, sialic acid exists on the cell membrane surface in the form of oligosaccharides, glycolipids, and glycoproteins, participating in cell signal reception and transmission, and is known as the "cell antenna." Based on multiple in vitro (antioxidant efficacy evaluation, type I collagen content, tyrosinase activity inhibition experiment, PGE-2 inflammatory factor content determination) and human efficacy tests, the research team of this invention has verified that sialic acid has moisturizing, firming, and soothing effects. Furthermore, it can scavenge free radicals through multiple pathways, reducing oxidative damage, and also has anti-inflammatory effects, protecting the skin from external stimuli. This is especially important for fragile skin after exfoliation, and can prevent post-inflammatory hyperpigmentation.

[0021] Based on the aforementioned theoretical facts, the research and development team of this invention further discovered through exploratory experiments that sialic acid has an excellent and gentle exfoliating effect, enabling a dynamic balance skincare strategy of "breaking down and rebuilding simultaneously." Even with a certain concentration of sialic acid, it can gently soften dead skin cells, promote exfoliation and the penetration and absorption of sialic acid, allowing it to fully exert its skin-protecting function and solving the pain points of traditional acid-based products that either "only break down but don't rebuild" or "break down first and then rebuild."

[0022] In a first aspect, embodiments of the present invention provide a mild sialic acid exfoliating composition comprising sialic acid and a solvent, wherein the mass concentration of sialic acid is 2%-15% and the solvent is selected from at least one of 1,3-propanediol, butanediol, dipropylene glycol and water.

[0023] It should be noted that the sialic acid gentle exfoliation composition has a simple formula. The sialic acid in the composition is minimally irritating to the skin. It can gently soften dead skin cells, promote exfoliation and the absorption of sialic acid, allowing sialic acid to fully exert its skin-protecting function. It has excellent gentle exfoliation effect and can improve pigmentation and enlarged pores. It is conducive to achieving a dynamic balance skincare strategy of "breaking down and rebuilding simultaneously".

[0024] In an optional embodiment, the sialic acid mild acid-exfoliating composition consists of sialic acid at a mass concentration of 2%-10% and a solvent; Preferably, the mass concentration of sialic acid is 5%-10%. For example, the mass concentration of sialic acid can be selected from any one of 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 9% and 10%, or other values ​​within the range of 5%-10%.

[0025] Secondly, embodiments of the present invention provide a toner comprising component A and component B, with the remainder being water, wherein component B comprises the composition as described in the foregoing embodiments. By weight, component A includes 3%-10% humectant, 0.5%-1.5% preservative, and 0.3%-0.8% thickener; component B includes 0.01%-20% sialic acid mild exfoliating composition, 0.2%-1.5% pH adjuster, and 0.5%-1.5% preservative.

[0026] Furthermore, the pH adjuster is 0.2%-1.2% by weight.

[0027] It should be noted that there are many applicable substances for moisturizers, preservatives, thickeners, compositions, pH adjusters, and preservatives, and the specific types of substances can be reasonably replaced according to actual needs. In the embodiments of the present invention, the raw materials for preparing toner include water, thickener (bentonite), moisturizer (glycerin, butylene glycol), preservative (p-hydroxyacetophenone, 1,2-hexanediol), and pH adjuster (arginine).

[0028] Thirdly, embodiments of the present invention provide a method for preparing a toner as described in the foregoing embodiments, comprising the following preparation steps: Mix component A in the specified proportion, heat to 80℃-85℃, stir until completely dissolved, add premixed component B, stir evenly, and cool to room temperature to obtain toner.

[0029] It should be noted that the preparation method of toner is simple, and the raw materials used in the preparation process are single. The toner made will not cause skin irritation, redness, dryness, peeling, or damage to the skin barrier during use. It is beneficial to improve pigmentation and pore management, thereby improving skin condition.

[0030] In an optional embodiment, the preparation method further includes: heating the mixed component A to 80℃-85℃ and holding it at that temperature for 8min-20min, then cooling it to 40℃-45℃, and then adding component B.

[0031] It should be noted that the thickener in component A needs to swell and form micelles in hot water. Heating reduces the intermolecular forces between the thickener, preservative, and other materials, increasing solubility and allowing them to dissolve fully, resulting in a homogeneous and clear mixed solution of component A. The initial heating treatment can temporarily destroy some microorganisms in component A during the high-temperature stage. After cooling, the system viscosity increases, and with the appropriate amount of preservative, the growth of residual microorganisms can be inhibited. The subsequent cooling treatment is to stabilize the component A system and effectively prevent the active ingredient of sialic acid in component B from being deactivated at high temperatures, thus avoiding loss of function; it also prevents overheating reactions or the formation of byproducts.

[0032] Fourthly, embodiments of the present invention provide a cosmetic product comprising a mild acid-exfoliating composition of sialic acid as described in the foregoing embodiments.

[0033] It should be noted that cosmetics, because they include compositions, are all types of cosmetics that gently exfoliate and regulate the stratum corneum. Furthermore, the cosmetics provided in the embodiments of the present invention have the following characteristics: clear and refreshing, exfoliating, softening stratum corneum, pore management, and improvement of pigmentation.

[0034] In an optional embodiment, the method for preparing the cosmetic, using the raw materials of the cosmetic described in the aforementioned embodiment, includes: mixing the raw materials evenly.

[0035] In an optional embodiment, the type of cosmetic is selected from at least one of aqueous solutions, emulsions, creams, masks, gels, and serums; Preferably, the aqueous solution includes the toner as described in the foregoing embodiments or the toner prepared by the foregoing preparation method.

[0036] Preferably, by weight percentage, the cosmetic comprises: a mild sialic acid exfoliating composition, at least one of a thickener, emulsifier, emollient, preservative, active ingredient, and pH adjuster.

[0037] Preferably, by mass percentage, the mild acid-exfoliating composition contains 0.1%-20% sialic acid, 0.1%-2% thickener, 1%-2% emulsifier, 5%-20% emollient, and 0.1%-1.5% preservative; and the pH adjuster is 0.25-0.6 times the total mass of sialic acid.

[0038] Preferably, the active ingredients include at least one of antioxidants, moisturizers, anti-wrinkle agents, whitening agents, soothing agents, skin conditioning agents, exfoliants, and penetration enhancers.

[0039] In optional embodiments, the emulsifier is selected from polyglycerol-6 stearate, polyglycerol-6 behenate, PEG-100 stearate, glyceryl stearate, cetearyl oleate, sorbitan oleate, sorbitan oleate, glyceryl stearate citrate, cetyl phosphate potassium, sodium stearoyl glutamate, cetearyl glucoside, C12-20 alkyl glucoside, stearyl alcohol polyether-2, stearyl alcohol polyether-21, sorbitan stearate, polysorbate-20, polysorbate-60, polysorbate-80, tridecyl alcohol polyether-6, and PEG-60 hydrogenated castor oil. The oil, PEG-9 polydimethylsiloxane, sorbitan olive oil ester, PPG-13-decyltetradecyl alcohol polyether-24, dehydrated sorbitan monoisostearate, C10-C22 fatty acids, glyceryl monocitrate, glyceryl stearate, glyceryl stearate SE, isostearyl glyceryl ether, cetyl phosphate potassium, lecithin, methyl glucose sesquistearate, glyceryl monolactate, glyceryl monoacetate, glyceryl monocitrate, glyceryl monocitrate, diacetyl tartaric acid monoglyceride, glyceryl monolactate, sodium salt of lauryl sulfate and sodium cetyl phosphate.

[0040] Preferably, the emollient is selected from petrolatum, mineral oil, paraffin, microcrystalline wax, ceresin, squalane, dimethicone, cyclomethicone, cetyl alcohol, stearyl alcohol, lanolin, beeswax, candelilla wax, carnauba wax, shea butter, cocoa butter, mango butter, coconut oil, sodium hyaluronate, panthenol, trehalose, mannitol, sodium pyrrolidone carboxylate, sodium lactate, urea, amino acids, lactic acid, urea, inorganic salts, caprylic / capric triglycerides, isononyl isononanoate, isopropyl palmitate, isopropyl myristate, isodecyl neopentanoate, dioctyl carbonate, isopropyl isostearate, decyl oleate, isostearate... The following are included: lipid esters, cetearyl alcohol ethylhexanoate, pentaerythritol tetraisostearate, myristyl myristate, ethylhexyl palmitate, isocetyl stearate, cetyl lactate, polydimethylsiloxane, cyclodimethylsiloxane, phenyl polytrimethylsiloxane, polydimethylsiloxane alcohol, ammonia-terminated polydimethylsiloxane, various silicone elastomers / gels, oleic acid, linoleic acid, stearic acid, cetyl alcohol, stearyl alcohol, oleyl alcohol, jojoba seed, almond oil, avocado oil, sunflower seed oil, grape seed oil, olive oil, rosehip oil, squalane, hydrogenated polyisobutylene, hydrogenated polydecene, isododecane, and isohexadecane.

[0041] Preferably, the antioxidant is selected from at least one of vitamin C and its derivatives, vitamin E and its derivatives, coenzyme Q10 (ubiquinone), glutathione, superoxide dismutase, green tea extract (tea polyphenols), ferulic acid, resveratrol, astaxanthin, idebenone, ubiquinone, and ergothione; ergothione is preferred; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0042] Preferably, the anti-wrinkle agent is selected from at least one of retinol and its derivatives, peptides, serotonin, collagen, elastin and silk core protein; preferably collagen; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0043] Preferably, the whitening agent is selected from at least one of nicotinamide, glycyrrhizin, hexylresorcinol, phenylethylresorcinol, kojic acid, and tranexamic acid; preferably nicotinamide; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0044] Preferably, the soothing agent is selected from at least one of ectoine, allantoin, panthenol, dipotassium glycyrrhizate, asiaticoside, oat extract, patchouli extract, gentian extract, cynanchum atratum extract, purslane extract, and willowherb extract; ectoine is preferred; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0045] Preferably, the skin conditioning agent is selected from at least one of bird's nest extract, pearl extract, carnosine, hexapeptide-9, zinc PCA, and ceramide; preferably carnosine; in other embodiments of the present invention, other substances may be selected as needed.

[0046] Preferably, the exfoliating agent is selected from at least one of fruit acid, salicylic acid, hydroxyethylpiperazine ethanesulfonic acid, papain and bromelain; preferably hydroxyethylpiperazine ethanesulfonic acid; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0047] Preferably, the fruit acid is selected from at least one of glycolic acid, lactic acid, citric acid and mandelic acid.

[0048] Preferably, the penetration enhancer is selected from at least one of ethoxydiethylene glycol, azone, tetrahydropiperine, inositol, isosorbide dimethyl ether, and ethanol, with ethoxydiethylene glycol being the most preferred; in other embodiments of the present invention, other substances may be selected as needed.

[0049] In optional embodiments, the moisturizer is selected from at least one of glycerin, dipropylene glycol, butylene glycol, 1,3-propanediol, propylene glycol, 1,2-pentanediol, betaine, glyceryl glucoside, methyl glucetide 20, panthenol (vitamin B5), sodium pyrrolidone carboxylate, glyceryl polyether 26, trehalose, sucrose, mannitol, rhamnose, xylitol, hyaluronic acid, sodium hyaluronate, polyethylene glycol-8, methyl glucetide-10, PEG / PPG-17 / 6 copolymer, hydrolyzed sclerotium gum, budding stalk enzyme polysaccharide, tremella polysaccharide, sodium polyglutamate, PPG-10 methyl glucose ether, panthenol, and PPG-20 methyl glucose ether; preferably at least one of glycerin, betaine, glyceryl glucoside, trehalose, and sodium hyaluronate.

[0050] Preferably, the preservative is selected from at least one of capryloyl hydroxamic acid, 1,2-hexanediol, ethylhexylglycerin, sodium benzoate, 1,2-pentanediol, methylparaben, p-hydroxyacetophenone, phenoxyethanol, and chlorphenesin; more preferably, at least one of 1,2-hexanediol and p-hydroxyacetophenone; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0051] The thickener is selected from at least one of bentonite, xanthan gum, hydroxyethyl cellulose, acrylate / alkyl acrylate crosspolymer with 10-30 carbon atoms, acrylate / stearyl polyoxyethylene ether-20 itaconic acid copolymer, acrylate / hexadecyl polyoxyethylene ether-20 itaconic acid copolymer, acrylate / amino acrylate / alkyl PEG-20 itaconic acid copolymer with 10-30 carbon atoms, acrylate copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, ammonium acryloyl dimethyl taurate / behenol polyether-25 methacrylate crosspolymer, polyacrylate-1 crosspolymer, and acrylate / stearyl polyoxyethylene ether-20 methacrylate crosspolymer; preferably at least one of bentonite, xanthan gum, and hydroxyethyl cellulose; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0052] The pH adjuster is selected from at least one of triethanolamine, arginine, sodium hydroxide, potassium hydroxide, tromethamine, citric acid, and sodium citrate; preferably arginine; in other embodiments of the present invention, other substances may be selected according to actual needs.

[0053] Fifthly, embodiments of the present invention provide the application of a mild exfoliating composition of sialic acid as described in the foregoing embodiments, or a toner as described in the foregoing embodiments, or a toner prepared by the preparation method as described in the foregoing embodiments, or a cosmetic as described in the foregoing embodiments, in improving skin condition.

[0054] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0055] Example 1 This embodiment provides a mild sialic acid exfoliating composition, which is composed of sialic acid and water; the mass concentration of sialic acid is 2% by mass percentage, and it is referred to as the mild sialic acid exfoliating composition (I).

[0056] Example 2 This embodiment provides a mild sialic acid exfoliating composition, which is composed of sialic acid and water; the mass concentration of sialic acid is 5% by mass percentage, and it is referred to as mild sialic acid exfoliating composition (II).

[0057] Example 3 This embodiment provides a mild sialic acid exfoliating composition, which is composed of sialic acid and water; the mass concentration of sialic acid is 10% by mass percentage, and it is referred to as mild sialic acid exfoliating composition (III).

[0058] Comparative Example 1 This comparative example provides a conventional acid-scraping composition consisting of glycolic acid and water; the glycolic acid concentration is 5% by mass percentage.

[0059] Comparative Example 2 This comparative example provides a conventional acid-scraping composition consisting of lactic acid and water; the mass concentration of lactic acid is 5% by mass percentage.

[0060] Comparative Example 3 This comparative example provides a conventional acid-scraping composition consisting of citric acid and water; the mass concentration of lactobionic acid is 5% by mass percentage.

[0061] Application Example 1 This application example provides a toner whose components include the sialic acid mild exfoliating composition prepared in Example 2. The total volume of the toner is 100 mL, comprising component A, component B, and the remainder water, as shown in Table 1. In other application examples of this invention, the total volume of the toner can be reasonably adjusted according to actual needs, and the corresponding components A, B, and remainder water can also be adjusted accordingly.

[0062] The preparation method of the toner is as follows: Place component A in a mixing container according to the specified ratio, then add deionized water, heat to 83℃, stir until completely dissolved, keep warm for 10 minutes, then cool to 43℃. Mix component B evenly according to the specified ratio, then add component A, and continue stirring to dissolve. After stirring evenly, cool to room temperature to obtain the toner. It should be noted that the mixing container can be reasonably selected according to the actual amount of raw materials being processed; the actual amount of sialic acid in the mild sialic acid exfoliating composition (II) is 0.5%.

[0063] The resulting toner has a pH range of ≥3.5. The toner product is stable during storage, has a uniform appearance, and is clear and transparent. See details... Figure 1 As shown.

[0064] Table 1 Toner Formula

[0065] Test Example 1 This test case evaluates the safety of the mild acid-exfoliating compositions prepared in Examples 1-3 and the conventional acid-exfoliating compositions prepared in Comparative Examples 1-3.

[0066] Specific methods: Using qualified patch testing equipment and a closed patch test method, the sample was diluted tenfold with pure water to serve as the test substance. 0.020mL-0.025mL of the sample was placed in the patch testing device, and then applied externally with hypoallergenic adhesive tape to the flexor side of the subject's forearm. The test substance was removed after 24 hours. Skin reactions were observed at 0.5h, 24h, and 48h after removal, and the results were recorded according to the skin reaction grading standards in the *Cosmetic Safety Technical Specifications* (2015 edition). The mild sialic acid peeling compositions of Examples 1-3 and the traditional peeling compositions prepared in Comparative Examples 1-3 were used as samples. The skin reaction grading standards are shown in Table 2.

[0067] The subject information is as follows: 33 volunteers were selected as subjects, aged 20-45 years, with healthy skin, no history of skin diseases or allergies, and meeting the subject volunteer inclusion criteria. They were randomly divided into 9 groups for testing, with each group using one sample.

[0068] Table 2 Skin Reaction Grading Standards

[0069] Safety test results showed that all 33 subjects in Examples 1-3 had negative skin reactions, with a score of 0, indicating that sialic acid, as a novel exfoliating ingredient, is milder, safer, and non-irritating than traditional fruit acid exfoliating ingredients. The skin reactions of subjects with the mild sialic acid exfoliating compositions of Examples 1-3 and the traditional exfoliating compositions prepared in Comparative Examples 2-3 were all negative, with a score of 0, indicating no irritation. However, the subjects in Comparative Example 1 showed a slight erythema, with a score of 1, indicating that glycolic acid, a fruit acid component in traditional exfoliating ingredients, has a certain degree of irritation.

[0070] Test Example 2 This test example demonstrates the in vitro exfoliation efficacy of the sialic acid mild exfoliation compositions of Examples 1-2 and the conventional exfoliation compositions prepared in Comparative Examples 1-3.

[0071] It should be noted that under normal circumstances, as epidermal keratinocytes differentiate and migrate upwards, the outermost layer of keratinocytes gradually sheds. However, with increasing age, external stress, and environmental factors such as ultraviolet radiation can slow down skin metabolism, leading to abnormal keratinocyte shedding or renewal, resulting in adverse effects such as dull skin, roughness, lack of luster, and deepened wrinkles. Since protein is a major component of the stratum corneum, the amount of shed keratinocytes is directly proportional to the amount of shed protein. Therefore, this test uses isolated pig skin as a test model, employing quantification of shed protein and cell counting methods to comprehensively evaluate the exfoliating efficacy of the product composition.

[0072] (1) Experimental reagents and equipment (1.1) Experimental reagents: physiological saline (Sichuan Kelun Pharmaceutical Co., Ltd.), salicylic acid (Shanghai Aladdin Biochemical Technology Co., Ltd.), BCA protein concentration assay kit (Shanghai Beyotime Biotechnology Co., Ltd.). It should be noted that the manufacturers of the experimental reagents can make reasonable adjustments according to actual needs.

[0073] (1.2) Experimental equipment: Biosafety cabinet (Sujing Antai, BSC-1604ⅡA2), D-Squame cuticle patch (CuDerm), D-Squame D500 pressure device (CuDerm), KQ5200B ultrasonic cleaner (Kunshan Ultrasonic Instruments), hemocytometer (Shanghai Qiujing Biochemical Instruments), inverted microscope (Leica, DMi8), ELISA reader (Tecan, Spark). It should be noted that the manufacturers of the experimental equipment can make reasonable adjustments according to actual needs.

[0074] (2) Experimental methods (2.1) Model preparation: The isolated pig skin was taken out from the -20℃ freezing condition, placed in 0.9% physiological saline for 30 min to recover, and then the pig skin was removed with tweezers and dried at room temperature; (2.2) Group loading: According to the experimental design, take equal amounts of sample and apply them evenly to the surface of pigskin, and leave them for 10 minutes; (2.3) Patch sampling: D-Squame keratin patches were placed on the surface of pigskin. The sampler held the pressure device and pressed one end of the pressure device against the patch with a fixed pressure for 30 seconds. After removing the patch, it was inserted into a 1.5mL centrifuge tube without contamination. (2.4) Extraction of exfoliated keratin: Add 1.5 mL of physiological saline to soak the keratin patch, and then sonicate for 15 min; (2.5) BCA protein concentration test: Take the extract of keratinocytes shed from each group of keratinocyte patches after ultrasound, and perform quantitative test of keratinocyte exfoliation protein BCA protein concentration according to the kit instructions; Among them, the blank control group is the number of keratinocytes shed from isolated pig skin after treatment with deionized water, and the groups of Example 1, Example 2, Comparative Example 1, Comparative Example 2 and Comparative Example 3 are the number of keratinocytes shed from isolated pig skin after treatment with 10% aqueous solution prepared for the corresponding sample groups.

[0075] Based on the above experimental methods, the exfoliated keratin extract was collected, and the BCA protein concentration was detected. The results are shown in Table 3.

[0076] Table 3 Results of BCA protein concentration test

[0077] Note: "**" indicates P≤0.01, meaning there is a significant difference compared to the blank control.

[0078] As shown in Table 3, compared with the blank control, the content of exfoliated protein in the sample groups of Examples 1-2 and Comparative Examples 1-3 increased significantly, indicating that both the sialic acid in the mild sialic acid exfoliating composition and the fruit acid components in the traditional exfoliating composition have the effect of exfoliation. However, the exfoliation effect of Examples 1-2 is better than that of Comparative Examples 1-3, indicating that sialic acid can play an exfoliating role in a lower concentration range and has the potential for safety and high efficiency.

[0079] It should be noted that the sialic acid mild exfoliating composition from Example 2 was subsequently used to prepare a toner for human efficacy verification.

[0080] Test Example 3 This test example demonstrates the transdermal moisture loss test of the compositions prepared in Examples 1-2 and Comparative Examples 1-3.

[0081] It's important to note that water and barrier function are crucial aspects of skin barrier function. Reducing transepidermal water loss (TEWL) helps maintain adequate skin hydration. Lipid components in the stratum corneum, particularly ceramides, are essential for maintaining the skin's water-retention capacity. A healthy skin barrier keeps TEWL at a low level (typically <30 g / m²). 3 •h). The standard TEWL is measured under conditions of dry, sweat-free skin, at an ambient temperature of 24°C and an ambient humidity of 50%. All test data in this study were obtained under these conditions.

[0082] The formula for calculating the TEWL value decline rate is as follows: TEWL value decrease rate (%) = (A0 - A n) / A0×100%, where A0 is the TEWL value in week 0, A n This is the TEWL value for week n.

[0083] A positive TEWL value decrease rate indicates that the skin barrier has been repaired, while a negative TEWL value decrease rate indicates that the skin barrier has not been repaired. Furthermore, the greater the TEWL value decrease rate, the better the skin barrier repair effect.

[0084] The formula for calculating the average rate of decline of the TEWL value is as follows: The average TEWL value decline rate (%) = the sum of the TEWL value decline rates of each group / the number of people in that group.

[0085] Thirty-three volunteers, aged 20-45 years, with healthy skin and no history of skin allergies, met the inclusion criteria for volunteer participation. They were randomly divided into nine groups for testing, with each group using one sample. The mild sialic acid peeling compositions of Examples 1-2 and the traditional peeling compositions prepared in Comparative Examples 1-3 were used as samples. Participants applied an equal amount of sample (0.5 g) evenly to the inner forearm twice daily, morning and evening. The same tester then used a TM300 analyzer from CK (Germany) to measure transepidermal water loss (TEWL) on the inner forearm at week 0 and after continuous use until week 2 and week 4. The test results are shown in Table 4.

[0086] Table 4 Results of Skin Barrier Function Test

[0087] As shown in Table 4, compared with Comparative Examples 1-3, the average decrease rate of TEWL values ​​in Examples 1-2 was higher, with Example 2 showing the highest average decrease rate, indicating that Example 2 had the best skin barrier repair effect. Therefore, this invention uses Example 2 as the preferred formulation of the skin barrier repair composition with synergistic skin resurfacing. The average decrease rate of TEWL values ​​in Comparative Examples 1-3 was significantly lower than that in Examples 1-3, indicating that traditional acid-based exfoliating ingredients, such as fruit acids, are not as effective as sialic acid in repairing the skin barrier.

[0088] Test Example 4 This test case compares the skincare effects of the toner prepared in Case 1 before and after human efficacy testing, focusing on skin pigmentation and pore size.

[0089] The specific method is as follows: Qualified subjects are selected according to inclusion and exclusion criteria to ensure that the final number of valid cases is no less than 30. A single-center, single-blind clinical trial design is adopted. Objective indicator tests and subjective assessments are conducted using a full-face before-and-after control method, and full-face / partial photographs are taken. The trial results are compared using statistical tests to determine whether there are statistically significant differences.

[0090] Inclusion criteria: Healthy men / women aged 18-50; have needs for exfoliation, pore refinement, and pigmentation improvement; can cooperate well with the participants and maintain a regular lifestyle during the study; can read and understand all contents of the informed consent form and voluntarily sign the informed consent form; agree not to use any cosmetics, drugs, or health products that may affect the results during the study.

[0091] Indicator Testing: This invention tested skin-related indicators at different time points (day 0 and day 28). The tests used T / CAB 0152-2022, "Test Methods for Seven Efficacy Items of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing, and Soothing," to measure facial skin redness (a*) value, percentage of red area, skin pigmentation, and degree of redness. Valid results for the pigmentation test are shown below. Figure 2 2a-2b represent the forehead area, and 2c-2d represent the corners of the eyes. 2a and 2c are results from day 0, and 2b and 2d are results from day 28. See below for effective examples of pore size testing. Figure 3 Among them, 3a and 3c are the test results after 0 days, and 3b and 3d are the test results after 28 days.

[0092] Combination Figure 2 and Figure 3 The results of the effective cases show that after the subjects used the toner in Case 1 for 28 consecutive days, their pigmentation improved by 25.68% and their total pore volume decreased by 20.50%.

[0093] Test Example 5 This test case involved subjective evaluation of consumer use of the toner in Application Example 1 for 28 days. The evaluation indicators and results are shown in Table 5. Table 5. Subjective evaluation results of consumers after 28 days of using the toner in test application example 1.

[0094] Note: Evaluation criteria: 1 point for "Strongly Disagree", 2 points for "Disagree", 3 points for "Neutral", 4 points for "Agree", and 5 points for "Strongly Agree"; Sample size: 33. "***" indicates P≤0.001, meaning there is a significant difference before and after using the toner.

[0095] As shown in Table 5, after 28 days of continuous use of the toner prepared in Example 2 containing 5%, compared with the baseline on day 0, 87.88% of the subjects agreed that the product gently cleansed the stratum corneum, 87.88% agreed that the product improved the health of the skin barrier, 90.91% agreed that the product increased the smoothness and delicacy of the skin, and 93.94% agreed that the product improved closed comedones and acne caused by keratin buildup. Therefore, it is believed that the product has the effects of keratin regulation, pore conditioning, and skin refinement.

[0096] In summary, compared with traditional exfoliating ingredients, the sialic acid gentle exfoliating composition provided by this invention, as a novel gentle exfoliating agent, has better moisturizing properties and safety. It can form a three-dimensional system of "exfoliation-repair-defense" by exerting the effects of multiple active groups. After gently exfoliating dead skin cells, it simultaneously repairs the skin barrier through moisturizing, improves pigmentation through anti-oxidation, improves enlarged pores by stimulating collagen remodeling and supporting structure, and finally consolidates the healthy state of the skin through immune regulation.

[0097] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A mild acid-exfoliating composition containing sialic acid, characterized in that, The mild acid-exfoliating composition comprises sialic acid and a solvent, wherein the mass concentration of the sialic acid is 2%-15%, and the solvent is selected from at least one of 1,3-propanediol, butanediol, dipropylene glycol, and water.

2. The mild acid-exfoliating composition for bird's nest acid according to claim 1, characterized in that, The mild acid-exfoliating composition for bird's nest acid consists of bird's nest acid at a mass concentration of 2%-10% and the solvent; Preferably, the mass concentration of sialic acid is 5%-10%.

3. A toner, characterized in that, The toner comprises component A and component B, with the remainder being water, wherein component B comprises the sialic acid mild exfoliating composition as described in any one of claims 1-2. By weight percentage, component A comprises 3%-10% humectant, 0.5%-1.5% preservative, and 0.3%-0.8% thickener; component B comprises 0.01%-20% sialic acid mild exfoliating composition, 0.2%-1.5% pH adjuster, and 0.5%-1.5% preservative.

4. A method for preparing the toner as described in claim 3, characterized in that, The preparation steps include the following: Mix component A in the specified proportions, heat to 80℃-85℃, stir until completely dissolved, add premixed component B, and stir evenly to obtain toner.

5. The preparation method according to claim 4, characterized in that, The preparation method further includes: heating the mixed component A to 80℃-85℃ and holding it at that temperature for 8min-20min, then cooling it to 40℃-45℃, and then adding component B.

6. A cosmetic product, characterized in that, The cosmetic product includes the sialic acid mild exfoliating composition as described in any one of claims 1-2.

7. The cosmetic product according to claim 6, characterized in that, The cosmetics are selected from at least one of the following: aqueous solutions, emulsions, creams, masks, gels, and serums. Preferably, the aqueous solution comprises the toner as described in claim 3 or the toner prepared by the preparation method as described in any one of claims 4-5; Preferably, the cosmetic comprises: a mild exfoliating composition containing sialic acid, and at least one of a thickener, emulsifier, emollient, preservative, active ingredient, and pH adjuster; preferably, by mass percentage, the mild exfoliating composition containing sialic acid comprises 0.1%-20%, the thickener 0.1%-2%, the emulsifier 1%-2%, the emollient 5%-20%, and the preservative 0.1%-1.5%; based on the total mass of sialic acid, the mass of the pH adjuster is 0.25-0.6 times the total mass of sialic acid; Preferably, the active ingredient includes at least one of antioxidants, moisturizers, anti-wrinkle agents, whitening agents, soothing agents, skin conditioning agents, exfoliants, and penetration enhancers.

8. The cosmetic product according to claim 7, characterized in that, The emulsifier is selected from polyglycerol-6 stearate, polyglycerol-6 behenate, PEG-100 stearate, glyceryl stearate, cetearyl oleate, sorbitan oleate, sorbitan oleate, glyceryl stearate citrate, cetyl phosphate potassium, sodium stearoyl glutamate, cetearyl glucoside, C12-20 alkyl glucoside, stearyl alcohol polyether-2, stearyl alcohol polyether-21, sorbitan stearate, polysorbate-20, polysorbate-60, polysorbate-80, tridecyl alcohol polyether-6, and PEG-60 hydrogenated castor oil. At least one of the following: sesame oil, PEG-9 polydimethylsiloxane, PPG-13-decyltetradecyl alcohol polyether-24, sorbitan monoisostearate, C10-C22 fatty acids, glyceryl monocitrate, glyceryl stearate, glyceryl stearate SE, isostearyl glyceryl ether, cetyl phosphate potassium, lecithin, methylglucose sesquistearate, glyceryl monolactic acid, glyceryl monoacetate, glyceryl monocitrate, diacetyl tartaric acid, glyceryl monolactic acid, sodium salt of lauryl sulfate, and sodium cetyl phosphate; Preferably, the emollient is selected from petrolatum, mineral oil, paraffin wax, microcrystalline wax, ceresin wax, squalane, dimethicone, cyclomethicone, cetyl alcohol, stearyl alcohol, lanolin, beeswax, candelilla wax, carnauba wax, shea butter, cocoa butter, mango butter, coconut oil, sodium hyaluronate, panthenol, trehalose, mannitol, sodium pyrrolidone carboxylate, sodium lactate, urea, amino acids, lactic acid, urea, inorganic salts, caprylic / capric triglycerides, isononyl isononanoate, isopropyl palmitate, isopropyl myristate, isodecyl neopentanoate, dioctyl carbonate, isopropyl isostearate, decyl oleate, isostearate... Stearyl ester, cetearyl alcohol ethylhexanoate, pentaerythritol tetraisostearate, myristyl myristate, ethylhexyl palmitate, isocetyl stearate, cetyl lactate, polydimethylsiloxane, cyclodimethylsiloxane, phenyl polytrimethylsiloxane, polydimethylsiloxane alcohol, ammonia-terminated polydimethylsiloxane, various silicone elastomers / gels, oleic acid, linoleic acid, stearic acid, cetyl alcohol, stearyl alcohol, oleyl alcohol, jojoba seed, almond oil, avocado oil, sunflower seed oil, grape seed oil, olive oil, rosehip oil, squalane, hydrogenated polyisobutylene, hydrogenated polydecene, isododecane, and isohexadecane; Preferably, the antioxidant is selected from at least one of vitamin C and its derivatives, vitamin E and its derivatives, coenzyme Q10, glutathione, superoxide dismutase, green tea extract, ferulic acid, resveratrol, astaxanthin, idebenone, ubiquinone, and ergothione; preferably ergothione. Preferably, the anti-wrinkle agent is selected from at least one of retinol and its derivatives, peptides, styroxin, collagen, elastin, and silk core protein; preferably, collagen. Preferably, the whitening agent is selected from at least one of nicotinamide, glycyrrhizin, hexylresorcinol, phenylethylresorcinol, kojic acid, and tranexamic acid; preferably, nicotinamide; Preferably, the soothing agent is selected from at least one of ectoine, allantoin, panthenol, dipotassium glycyrrhizate, asiaticoside, oat extract, patchouli extract, gentian extract, cynanchum atratum extract, purslane extract, and willowherb extract; preferably ectoine; Preferably, the skin conditioning agent is selected from at least one of bird's nest extract, pearl extract, carnosine, hexapeptide-9, zinc PCA, and ceramide; preferably carnosine. Preferably, the exfoliating agent is selected from at least one of fruit acid, salicylic acid, hydroxyethylpiperazine ethanesulfonic acid, papain, and bromelain; preferably, hydroxyethylpiperazine ethanesulfonic acid; preferably, the fruit acid is selected from at least one of glycolic acid, lactic acid, citric acid, and mandelic acid; Preferably, the penetration enhancer is selected from at least one of ethoxydiethylene glycol, azone, tetrahydropiperine, inositol, isosorbide dimethyl ether, and ethanol, and is preferably ethoxydiethylene glycol.

9. The toner according to claim 3 or the cosmetic according to claim 7, characterized in that, The moisturizer is selected from at least one of glycerin, dipropylene glycol, butylene glycol, 1,3-propanediol, propylene glycol, 1,2-pentanediol, betaine, glyceryl glucoside, methyl glucetide 20, panthenol, sodium pyrrolidone carboxylate, glyceryl ether 26, trehalose, sucrose, mannitol, rhamnose, xylitol, hyaluronic acid, sodium hyaluronate, polyethylene glycol-8, methyl glucetide-10, PEG / PPG-17 / 6 copolymer, hydrolyzed sclerotium gum, budding stalk enzyme polysaccharide, tremella polysaccharide, sodium polyglutamate, PPG-10 methyl glucose ether, panthenol, and PPG-20 methyl glucose ether; preferably at least one of glycerin, betaine, glyceryl glucoside, trehalose, and sodium hyaluronate. Preferably, the preservative is selected from at least one of capryloyl hydroxamic acid, 1,2-hexanediol, ethylhexylglycerin, sodium benzoate, 1,2-pentanediol, methylparaben, p-hydroxyacetophenone, phenoxyethanol, and chlorphenesin; more preferably, at least one of 1,2-hexanediol and p-hydroxyacetophenone. The thickener is selected from at least one of bentonite, xanthan gum, hydroxyethyl cellulose, acrylate / 10-30 carbon alkyl acrylate crosspolymer, acrylate / stearyl polyoxyethylene ether-20 itaconic acid copolymer, acrylate / hexadecyl polyoxyethylene ether-20 itaconic acid copolymer, acrylate / amino acrylate / 10-30 carbon alkyl PEG-20 itaconic acid copolymer, acrylate copolymer, ammonium acryloyl dimethyl taurate / VP copolymer, ammonium acryloyl dimethyl taurate / behenol polyether-25 methacrylate crosspolymer, polyacrylate-1 crosspolymer, and acrylate / stearyl polyoxyethylene ether-20 methacrylate crosspolymer; preferably at least one of bentonite, xanthan gum, and hydroxyethyl cellulose. The pH adjuster is selected from at least one of triethanolamine, arginine, sodium hydroxide, potassium hydroxide, tromethamine, citric acid, and sodium citrate; preferably arginine.

10. The use of a mild exfoliating composition of sialic acid as described in claim 1, or a toner as described in claim 3, or a toner prepared by the preparation method described in any one of claims 4-5, or a cosmetic as described in any one of claims 6-9, in improving skin condition.

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