Application of polygonatum cyrtonema cyclase PcAS1 in promotion of hematoxylin A synthesis

By mining the PcAS1 gene of Polygonatum cyclase and constructing a plant expression system, the biosynthesis of hematoxylin A in Polygonatum cyclase was successfully realized, solving the problem of unclear synthesis pathway of high isoflavones and promoting the industrial utilization of high isoflavone natural products.

CN120989129APending Publication Date: 2025-11-21ZHEJIANG FORESTRY UNIVERSITY +1
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Patent Information

Application Number
CN202511092689.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-05
Publication Date
2025-11-21

AI Technical Summary

Technical Problem

The existing synthetic pathway of high isoflavone hematoxylin A is unclear, and the key catalytic enzyme is unknown, which limits the biosynthesis and industrial utilization of high isoflavones.

Method used

The PcAS1 gene was discovered using high-throughput resequencing, transcriptomics, and metabolomics techniques in Polygonatum multiflorum. A transient transformation system for tobacco and a stable genetic transformation system for Polygonatum multiflorum root development were constructed to express the PcAS1 gene and promote the synthesis of hematoxylin A.

Benefits of technology

The biosynthesis of high isoflavone hematoxylin A was achieved, breaking through the bottleneck of synthetic research, providing an efficient synthetic system, and laying the technical foundation for the industrial application of high isoflavone natural products.

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Abstract

The invention discloses an application of polygonatum cyrtonema cyclase PcAS1 in promoting synthesis of hematoxylin A. The cyclase PcAS1 is derived from polygonatum cyrtonema, a high isoflavone compound hematoxylin A is synthesized in a plant body, and according to the application, a recombinant plant expression vector for expressing a PcAS1 gene is constructed and transformed into the plant to realize synthesis of the hematoxylin A. Based on multiple omics data such as whole genome, re-sequencing and transcriptome of polygonatum cyrtonema, key cyclase PcAS1 closely related to homoisoflavone biosynthesis is systematically mined. Through a tobacco transient expression system and a polygonatum cyrtonema hairy root transformation system, it is proved for the first time that cyclase PcAS1 can significantly promote synthesis of high isoflavone hematoxylon A, and biosynthesis of hematoxylon A is achieved in polygonatum cyrtonema for the first time.
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