Screening method for transparent bottled health-care wine containers

Abstract

The invention discloses a screening method for transparent bottled health-care wine containers, and aims to solve the problem that active ingredients of transparent bottled health-care wine are degraded due to illumination. According to the method, 200-700nm is taken as a research range, and the 200-700nm is divided into a plurality of equal-width sub-wavebands; detecting the absorbance sum Ak of the health-care wine in each sub-wave band and the average light transmittance Tk of the container in the corresponding sub-wave band; the equivalent light transmittance Te is calculated through a formula, the lower the Te value is, the better the protection effect of the containers on the light stability of the wine body is, and the containers with Te smaller than or equal to a preset threshold value are screened. According to the method, the light transmission characteristic of the container and the light sensitivity characteristic of the wine body are coupled, the evaluation accuracy is superior to the traditional single wavelength / average light transmittance index, the stability of active ingredients of the health wine in storage and display can be ensured, and the container practicability and the product quality are both considered.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of optimization of wine packaging, and specifically provides a screening method for a transparent bottle health-care wine container. BACKGROUND

[0002] With the increasing demand of consumers for a healthy life, plant health-care wine, as a functional and sensory product, has a growing market demand. Such health-care wine usually uses high-quality base wine as the base material, and adds extraction juice of herbal medicines such as angelica, cistanche and medlar. The functional value of the health-care wine mainly depends on active ingredients in the wine body, including flavonoids, polysaccharides, saponins, verbascoside, echinacoside, ferulic acid, ligustilide and other photosensitive trace components.

[0003] However, in the offline sales link of health-care wine, merchants generally use transparent glass bottles for product display and consumer observation. Although the transparent glass bottle has the advantages of low cost, excellent physical and chemical stability, and good ornamental value, it cannot effectively isolate environmental light, especially sunlight with a wavelength of 200nm-700nm (the light energy of this wavelength band can cause chemical bond rupture), which can cause photo-degradation of flavonoids and photosensitive trace components in the wine body. Under long-term light, the content of these active ingredients will significantly decrease, not only weakening the functional value of the health-care wine, but also possibly affecting the taste and safety of the product due to the generation of degradation products, which seriously restricts the quality stability of the transparent bottle health-care wine.

[0004] In the prior art, the protection scheme for photosensitive substances is mostly based on the experience of the pharmaceutical industry: in the pharmaceutical field, deep-colored non-transparent packaging such as brown bottles is used to reduce the impact of light on the active ingredients of medicines; the health-care wine industry continues this idea and selects transparent glass bottles with low single-wavelength light transmittance (such as ultraviolet light 365nm, visible light 550nm, infrared light 940nm) or 200nm-700nm average light transmittance (T0) as the core screening index.

[0005] However, the above method has significant defects: on the one hand, the wavelength distribution of light in the actual environment is extensive (not single wavelength or average distribution), and the light transmittance of glass for different wavelengths of light is different. Only using single wavelength or average light transmittance to evaluate the light shielding effect of the container, the detection value deviates greatly from the actual light transmittance under the light, and the data representativeness is insufficient; on the other hand, different health wine bodies have essential differences in sensitivity to specific wavelength light due to the difference in base wine degree and the proportion of medicinal material extract juice added - for example, the wine body with high medicinal material addition amount is more sensitive to 300nm-350nm waveband light, and the low degree wine body reacts more significantly to 400nm-450nm waveband light. The existing method only focuses on the light transmittance characteristics of the container itself, completely ignores the light absorption difference of the wine body, leading to a serious mismatch between the "container light transmittance ranking" and the "actual decay rate of active ingredients in the wine body", and cannot accurately screen out containers with both protection effect and practicality.

[0006] In summary, the existing screening scheme with "container single light transmittance" as the core cannot solve the core problem of "poor light stability" of transparent bottle health wine, and an integrated evaluation method combining "container light transmittance characteristics" and "sensitivity characteristics of wine body to light" is urgently needed to provide a scientific basis for the screening of transparent bottle health wine containers. SUMMARY

[0007] Therefore, the present application provides a screening method for transparent bottle health wine containers, which aims to overcome the defects of the existing screening method for transparent bottle health wine containers and solve the following core technical problems: The existing method only uses single wavelength light transmittance or average light transmittance of the container as an index, does not cover the key light waveband of 200nm-700nm in the actual environment, resulting in a large deviation between the light transmittance detection result and the actual protection effect of the container under real light, and insufficient evaluation accuracy; The existing method separates the "container" and "wine body", only focuses on the light shielding ability of the container itself, ignores the sensitivity difference of different wine bodies to specific wavelength light, and leads to a mismatch between the container screening result and the actual decay of active ingredients in the wine body, which cannot achieve targeted protection; The existing method often excessively relies on dark bottles (such as brown bottles) in pursuit of light shielding effect, which is difficult to balance the ornamental and cost control of the container, and limits the market display and enterprise production efficiency of transparent bottle health wine.

[0008] The technical solution of the present application is implemented as follows: the present application provides a screening method for transparent bottled health care wine containers, and the core idea of the present application is that the “transparent container” and “health care wine body” are regarded as an organic whole, the “wavelength range of 200-700 nm that can cause chemical bond breakage in sunlight” is taken as the research object, the sensitivity (absorbance characteristic) of the wine body to different sub-band light and the light shielding ability (transmittance characteristic) of the container to the corresponding sub-band are combined, the “equivalent transmittance (Te)” evaluation index is constructed, and the precise quantification of the protection effect of the container is realized, and the specific technical solution is as follows: Wavelength range and sub-band division The research wavelength range is determined to be 200-700 nm (this range is the key band leading to photo-degradation of active ingredients of the wine body), and the range is evenly divided into n continuous and non-overlapping sub-bands (n≥2, preferably n=10), wherein k is the sub-band index, and the value is 1-n (when n=10, the wavelength range of each sub-band is determined according to the formula (150+50k) nm-(200+50k) nm, and the sub-bands are 200-250 nm, 250-300 nm, 300-350 nm, 350-400 nm, 400-450 nm, 450-500 nm, 500-550 nm, 550-600 nm, 600-650 nm, and 650-700 nm).

[0009] Detection of total absorbance (A k ) of wine body 5 ml of health care wine sample to be evaluated is taken into a 50 ml volumetric flask, and the sample is diluted with pure water as a diluent to 50 mL to prepare a sample to be measured; using pure water as a blank control, the sample to be measured is scanned at a wavelength range of 200-700 nm with an interval of 1 nm by using a UV-visible spectrophotometer (preferably model TU-1901), and the absorbance data at each wavelength is recorded; the absorbance data of all wavelengths in each sub-band range is accumulated according to the sub-band range divided in step 1, and the total absorbance (A k ) corresponding to the sub-band is obtained. The higher the A k value, the stronger the sensitivity of the wine body to the light of the sub-band.

[0010] Detection of average transmittance (T k ) of container A glass sheet with the same thickness as the bottle body, no scratches, no bubbles and impurities is cut from the transparent container (such as a transparent glass bottle) to be screened; using air as a blank control, the glass sheet is scanned at a wavelength range of 200-700 nm with an interval of 1 nm by using a UV-visible spectrophotometer, and the transmittance data at each wavelength is recorded; the average value of the transmittance data of all wavelengths in each sub-band is calculated according to the sub-band range divided in step 1, and the average transmittance (Tk T k The lower the value, the stronger the container's ability to block light in that sub-band.

[0011] Calculation of equivalent transmittance (Te) and container selection Based on A obtained in step 2 k T obtained in step 3 k The equivalent transmittance (Te) of the container to be screened is calculated using the following formula:

[0012] The value of Te ranges from 0 to 100%. The lower the Te value, the better the protection effect of the container on the photosensitive active ingredients in the wine. According to the quality requirements of health wine (such as the active ingredient decay rate not exceeding the preset threshold), containers with Te ≤ target threshold are selected as the final transparent bottled health wine containers.

[0013] The present invention has the following advantages over the prior art: The accuracy of the evaluation has been significantly improved. The equivalent transmittance index proposed in this invention is the first to couple the calculation of "the transmittance of the container to different sub-wavelengths" with "the sensitivity of the wine to the corresponding sub-wavelengths," directly linking "the amount of light passing through the container" with "the degree to which the wine is affected by that light." This solves the problem of existing methods focusing only on the single transmittance of the container, which is disconnected from the actual protective effect. Experimental data shows that the ranking of Te is completely consistent with the actual attenuation rate of flavonoids and photosensitive trace components in the wine, providing a precise quantitative basis for container selection.

[0014] Balancing protective effectiveness with practical needs Compared to existing solutions that "simply pursue low light transmittance (such as brown bottles)," this invention can flexibly adapt to the actual needs of enterprises while ensuring that Te meets the protection threshold: for scenarios that pursue cost control, thin containers with the same bottle shape and Te compliance can be selected to reduce glass raw material consumption and production costs; for scenarios that emphasize market display, containers with Te compliance can be selected from bottle colors preferred by consumers (such as yellow, green, and blue), taking into account both product aesthetics and quality stability.

[0015] It has a wide range of applications and is highly operable. The technical solution of this invention does not rely on special equipment; it can be completed using only a conventional ultraviolet-visible spectrophotometer. k With T kThe detection is simple in operation process and good in repeatability; meanwhile, by adjusting the number (n>=2) of sub-bands and the target threshold of Te, the plant health care wine with different base liquor degrees and different medicinal material adding ratios can be adapted, and a general solution for container screening of various transparent bottled health care wines is provided, and the application prospect is wide.

[0016] Guarantee product quality and industry development The container screened by the present application can effectively delay the photodegradation of active ingredients of transparent bottled health care wine in the display and storage process, ensure the functional value and taste stability of the product from production to consumption end, improve the consumer experience, promote the market competitiveness of the transparent bottled health care wine category, and help the healthy development of the industry. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0018] Sample preparation Base liquor and medicinal material extract: The base liquor is 28-50 degree clear type liquor; the medicinal material extract is extracted from angelica, cistanche, jujube kernel, Chinese wolfberry, cassia bark and clove according to the following formula: Specific weight ratio: angelica 20%+cistanche 25%+jujube kernel 15%+Chinese wolfberry 20%+cassia bark 10%+cloves 10%, extraction process: after the medicinal materials are crushed, 60% ethanol is used for reflux extraction for 2h, and concentrated to relative density 1.05 (25℃).

[0019] Container samples: 5 kinds of 125ml transparent glass bottles (colorless, yellow, green, blue and brown), the bottle thickness is 2.0mm.

[0020] Detection instrument: TU-1901 type ultraviolet-visible spectrophotometer.

[0021] Experimental steps: (1) Wavelength range: 200nm-700nm, divided into 10 sub-bands (k=1-10, corresponding to 200-250nm to 650-700nm, divided according to (150+50k)nm-(200+50k)nm); (2) Ak detection: take 5ml wine body, add pure water to 50ml, take pure water as blank, scan at 1nm interval, calculate the sum of absorbance of each sub-band (Ak); k ​(3) Tk detection: intercept the container slide, air as blank, 1 nm interval scanning, calculate the average transmittance of each sub-band (T k (4) Te calculation: according to the original formula

[0022] (5) Light experiment: simulate natural light irradiation for 7 days, temperature 25±1℃, ultraviolet light (280-400nm) intensity 300±50μW / cm², visible light (400-700nm) intensity 7000±2000Lux.

[0023] Example 1 The basic parameters of the sample are as follows: The basic parameters of the sample are as follows:

[0024] The transmittance data of the container used are as follows: The basic parameters of the sample are as follows:

[0025] The sample absorbance weight data are as follows:

[0026] The sample Te value and decay rate data are as follows:

[0027] The data clearly show that the Te value and the decay rate of the active ingredient are strictly negatively correlated (such as the brown bottle of sample 1# Te=0.35%, the decay rate is the lowest; the colorless bottle Te=19.12%, the decay rate is the highest); The traditional indicators (T0, single-wavelength T) and the decay rate are not consistent (such as the blue bottle T0=20.08%<green bottle T0=25.80%, but the decay rate of the blue bottle of sample 1# is higher than that of the green bottle), while the Te value completely matches the decay rate ranking, proving the accuracy of the method.

[0028] Specifically: Average transmittance T0-brown (15.72%)<blue (20.08%)<green (25.80%)<yellow (31.56%)<colorless (68.67%) Single-wavelength transmittance T-brown (18.87%)<blue (34.80%)<green (44.63%)<yellow (53.10%)<colorless (85.27%) 1# sample ​Equivalent transmittance Te — Brown (0.39%) < Yellow (2.24%) < Green (2.42%) < Blue (2.69%) < Colorless (19.61%) Total flavone attenuation rate — Brown (4.29%) < Yellow (7.75%) < Green (9.29%) < Blue (10.60%) < Colorless (16.84%) Trace component average attenuation rate — Brown (14.53%) < Yellow (25.10%) < Green (29.01%) < Blue (29.74%) < Colorless (52.24%) 2# sample Equivalent transmittance Te — Brown (0.39%) < Yellow (2.24%) < Green (2.42%) < Blue (2.69%) < Colorless (19.61%) Total flavone attenuation rate — Brown (4.29%) < Yellow (7.75%) < Green (9.29%) < Blue (10.60%) < Colorless (16.84%) Trace component average attenuation rate — Brown (14.53%) < Yellow (25.10%) < Green (29.01%) < Blue (29.74%) < Colorless (52.24%) 3# sample Equivalent transmittance Te — Brown (0.45%) < Green (2.25%) < Blue (2.32%) < Yellow (2.38%) < Colorless (19.71%) Total flavone attenuation rate — Brown (4.65%) < Green (8.03%) < Blue (9.07%) < Yellow (9.89%) < Colorless (17.21%) Trace component average attenuation rate — Brown (12.15%) < Green (23.45%) < Blue (24.51%) < Yellow (26.48%) < Colorless (46.98%) 4# sample Equivalent transmittance Te — Brown (0.47%) < Green (2.44%) < Blue (2.55%) < Yellow (2.61%) < Colorless (22.05%) Total flavone attenuation rate — Brown (3.91%) < Green (7.82%) < Blue (9.00%) < Yellow (9.72%) < Colorless (14.64%) Trace component average attenuation rate — Brown (10.97%) < Green (22.69%) < Blue (24.99%) < Yellow (28.18%) < Colorless (48.37%) 5# sample Equivalent transmittance Te — brown (0.46%) < yellow (2.67%) < green (2.80%) < blue (3.04%) < colorless (22.64%) Total flavonoids attenuation rate — brown (3.10%) < yellow (6.66%) < green (7.72%) < blue (8.74%) < colorless (13.82%) Trace component average attenuation rate — brown (7.94%) < yellow (19.67%) < green (24.30%) < blue (26.05%) < colorless (44.77%) From the above experimental data, compared to the average transmittance (T0) or single wavelength transmittance (T) as the screening index of wine bottle, the equivalent transmittance (Te) can more accurately predict the degree of the influence of light transmittance through the container on the light stability of the wine components, and provide an effective basis for the screening of health wine bottles.

[0029] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A screening method of a transparent bottle health care wine container, characterized by, The protective effect of the container on the photostability of the wine is evaluated by calculating the equivalent transmittance Te of the wine container. The Te value is based on the absorbance weight A of the wine in the 200-700nm wavelength range. k The transmittance T of the container in the corresponding wavelength band k The equivalent transmittance Te is obtained, and a lower Te value indicates a better protective effect. The formula for calculating the equivalent transmittance Te is: Where n is the average number of sub-bands divided within the wavelength range of 200-700nm, n≥2, A k T represents the total absorbance of the wine in the k-th sub-band. k This represents the average transmittance of the container in the k-th sub-band.

2. The method of claim 1, wherein, The sub-wave bands are equally wide, and each sub-wave band has a width of 50 nm.

3. The method of claim 1, wherein, the wine body absorbance weight A k by the following steps: The health-care wine sample is diluted with pure water to constant volume; Using pure water as a blank control, using a UV-visible spectrophotometer to scan in a wavelength range of 200-700 nm at an interval of 1 nm, and recording absorbance data; Sum of absorbance of each band A is calculated by sub-band range k .

4. The method of claim 1, wherein, The container has a light transmission T k By the following steps: A glass sheet sample is cut from the container; Using air as a blank control, using a UV-visible spectrophotometer to scan in a wavelength range of 200-700 nm at an interval of 1 nm, and recording transmittance data; The average transmittance T of each waveband is calculated in the sub-waveband range k .

5. The method of claim 1, wherein, The n=10, and the specific wavelength ranges of the 10 continuous sub-wave bands are as follows: 200-250 nm when k=1, 251-300 nm when k=2, 301-350 nm when k=3, 351-400 nm when k=4, 401-450 nm when k=5, 451-500 nm when k=6, 501-550 nm when k=7, 551-600 nm when k=8, 601-650 nm when k=9, and 651-700 nm when k=10.

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