Fat-soluble composition containing sesame seed extract as well as preparation method and application of fat-soluble composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a fat-soluble composition containing a sesame seed extract as well as a preparation method and application of the fat-soluble composition. The sesame seed extract is compounded with at least two synergists in the tocopherol, the oryzanol and the rice germ extract, and a complementary synergistic mechanism can be formed among the components, so that the capabilities of scavenging free radicals and inhibiting lipid oxidation of the composition can be remarkably improved; the antioxidant effect of the composition is far better than that of a sesame seed extract or a single synergist which is independently used, the problems that a traditional fat-soluble antioxidant component is single in effect and insufficient in action strength are effectively solved, and more comprehensive oxidation protection is provided for a fat-soluble system; moreover, isosorbide dimethyl ether with a specific content is specifically selected as a solvent, and the dissolution characteristic of the isosorbide dimethyl ether can accurately adapt to the physicochemical properties of the sesame seed extract and the selected synergist, so that all active ingredients are ensured to be completely dissolved in the solvent, the precipitation phenomenon caused by the difference of the solubility of the ingredients is avoided, and the stability of the composition is ensured.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and particularly relates to a fat-soluble composition containing sesame seed extract, its preparation method, and its application. Background Technology

[0002] Sesame seed extracts have multiple sources and can be prepared by purification and enrichment from sesame seeds, sesame oil, and sesame cakes. The composition varies greatly depending on the source. According to the main research direction of the product, they can be divided into major categories such as fats, lignin compounds, proteins, carbohydrates, dietary fiber, vitamins, and minerals. The most common fields are health foods, pharmaceuticals, cosmetics, and other health-related fields.

[0003] In recent years, with the development of interdisciplinary and functional research, lignin compounds unique to sesame seed extract have been used in research. Common classifications of lignin include: sesamol, sesamin, isoseasaemin, sesaminlin, and sesaminlinol. Sesamol can be used as an antioxidant in oils but is a prohibited ingredient in cosmetics. Sesaminlin and sesaminlinol are derivatives of sesamin in sesame. Lignin is an important structural component of plant cell walls, forming lignin fibers together with cellulose. It is widely used in the field of biodegradable materials. Common uses of lignin in cosmetics include: ① effectively scavenging free radicals (such as DPPH and OH free radicals), inhibiting lipid peroxidation, and protecting cells from oxidative damage; ② enhancing BDNF (brain-derived neurotrophic factor) levels, supporting neuronal repair and growth, and improving cognitive function; ③ addressing issues such as lip aging, sagging lip skin, and increased lip lines; ④ improving hair color brightness and reducing frizz and other hair problems when applied to hair.

[0004] The academic community has identified fourteen major markers of aging, which include: (1) genomic instability; (2) telomere wear; (3) epigenetic changes; (4) loss of protein homeostasis; (5) autophagy dysfunction; (6) nutrient sensing disorder; (7) mitochondrial dysfunction; (8) cellular senescence; (9) changes in extracellular matrix; (10) stem cell depletion; (11) changes in intercellular communication; (12) chronic inflammation; (13) dysbiosis; and (14) psychosocial isolation. Among them, the core issues directly related to skin aging are concentrated on "cellular aging" (i.e., epidermal aging) and "mitochondrial dysfunction" (i.e., mitochondrial oxidative damage): Epidermal aging is mainly manifested as incomplete differentiation of adipocytes and abnormal keratinization of stratum corneum cells, which leads to disordered accumulation of epidermal layer structure, causing problems such as fine lines, skin aging, dull skin tone, and dryness; Mitochondrial oxidative damage will cause mitochondrial dysfunction, excessive consumption of endogenous oxidase SOD, and excessive oxidative damage of ROS (reactive oxygen species) will have a cumulative effect, further aggravating skin aging, specifically including promoting the production of matrix degradation enzymes, inducing brown spots, and causing the accumulation of lipofuscin, ultimately aggravating skin pigmentation and inflammatory response.

[0005] Although lignin compounds in sesame seed extract have shown certain antioxidant and repairing functions, and have been preliminarily applied in cosmetic lip care and hair care, existing technologies still have significant shortcomings: First, research and application targeting the core mechanisms of skin aging are limited—currently, the application of sesame seed extract in the cosmetic field is mostly concentrated on single functions (such as anti-aging for lips and hair care), without forming a systematic solution for the two key causes of skin aging, namely "epidermal aging (cellular senescence)" and "mitochondrial oxidative damage (mitochondrial dysfunction)," lacking specialized compositions that can synergistically address these two problems, making it difficult to achieve comprehensive anti-aging effects; Second, application areas... Insufficient Expansion – Existing research has not fully explored the potential of sesame seed extract as an anti-aging ingredient in cosmetics. Its application in developing ingredients for complex skin aging problems such as fine lines, dullness, pigmentation, and inflammation remains unexplored, resulting in a narrow range of applications for sesame seed extract in the field of cosmetic anti-aging and preventing it from entering the mainstream list of usable anti-aging ingredients. Thirdly, lack of research on fat-soluble compatibility – Some functional components in sesame seed extract (such as lignin compounds) are fat-soluble, but existing technologies have not designed specific compositions to address their fat-soluble characteristics. This prevents the full utilization of the advantages of fat-soluble components in skin penetration and long-lasting efficacy release, limiting the practical application of its anti-aging effects.

[0006] In summary, the current research and application of sesame seed extract in the field of cosmetic anti-aging still suffers from problems such as insufficient targeting, limited application scenarios, and incomplete release of efficacy. There is an urgent need to develop a fat-soluble composition of sesame seed extract that targets epidermal aging and mitochondrial dysfunction, in order to expand its application scope in the field of cosmetic anti-aging, supplement the list of anti-aging raw materials, and possess significant scientific research value and creativity. Summary of the Invention

[0007] The purpose of this invention is to overcome the shortcomings of the prior art and provide a fat-soluble composition containing sesame seed extract, its preparation method and application, with the aim of using a specific product composition to synergistically enhance the functional research of sesame seed extract on the surface of skin aging, mainly focusing on the overall impact of epidermal aging and mitochondrial oxidative damage on skin aging.

[0008] To achieve the above objectives, the technical solution adopted by the present invention includes: In a first aspect, the present invention provides a fat-soluble composition comprising sesame seed extract, a synergist, and a solvent, wherein the synergist is at least two of tocopherol, oryzanol, and rice germ extract; and the solvent comprises isosorbide dimethyl ether.

[0009] This invention utilizes sesame seed extract as the core active ingredient, combined with specific synergists to construct a fat-soluble composition system. Oryzanol effectively enhances the antioxidant capacity of sesame seed extract, thereby increasing its ability to scavenge ROS and reducing inflammation and cumulative oxidative damage. Rice germ extract, as a natural oil-soluble active ingredient that combats photoaging damage, effectively reduces the rate of epidermal aging and maintains skin homeostasis. Tocopherol (vitamin E), a fat-soluble antioxidant, effectively maintains the homeostasis of active ingredients in the oil-soluble system, improving product stability. Experimental studies have shown that when at least two of the aforementioned specific synergists are combined with sesame seed extract, the components synergistically enhance each other. Regarding epidermal aging, the prepared composition can significantly improve adipocyte differentiation, generate structural fat, reduce facial fine lines, and repair the epidermal barrier. Regarding mitochondrial dysfunction, the composition can significantly increase the types of free radicals scavenged and the antioxidant capacity, providing a novel technical pathway for fat-soluble compositions in the field of skin repair and anti-aging.

[0010] In addition, the present invention uses isosorbide dimethyl ether as a solvent, rather than a conventional fat-soluble solvent, which can better match the solubility characteristics of sesame seed extract and the above-mentioned synergists, ensuring that each component is uniformly dispersed in the system, further enhancing the stability and efficacy of the active ingredients, and avoiding the problems of component release and efficacy reduction caused by insufficient compatibility of conventional solvents.

[0011] Preferably, it includes at least one of the following (1)-(4): (1) The synergist is tocopherol and oryzanol, and the mass ratio of sesame seed extract to tocopherol and oryzanol is (2.5-5):(0.2-0.5):(0.1-0.2); more preferably, the mass ratio is 2.5:0.35:0.15; (2) The synergist is tocopherol and rice germ extract, and the mass ratio of sesame seed extract to tocopherol and rice germ extract is (2.5-5):(0.2-0.5):(0.25-0.75); more preferably, the mass ratio is 2.5:0.35:0.5; (3) The synergist is oryzanol and rice germ extract, and the mass ratio of sesame seed extract to oryzanol and rice germ extract is (2.5-5):(0.1-0.2):(0.25-0.75); more preferably, the mass ratio is 2.5:0.15:0.5; (4) The synergist is tocopherol, oryzanol and rice germ extract, and the mass ratio of sesame seed extract to tocopherol, oryzanol and rice germ extract is (2.5-5):(0.2-0.5):(0.1-0.2):(0.25-0.75); more preferably, the mass ratio is 2.5:035:0.15:0.5.

[0012] Experimental studies have shown that when at least two of the above-mentioned specific synergists are combined with sesame seed extract within the preferred range of this invention, a highly efficient synergistic effect can be formed among the components, thereby significantly improving the antioxidant capacity of the composition. Compared with the scheme of combining any one of the above three synergists with sesame seed extract alone, the "combination scheme of at least two specific synergists" defined in this invention has a significant advantage in antioxidant performance.

[0013] More preferably, the synergist is tocopherol, oryzanol and rice germ extract, and the mass ratio of sesame seed extract to tocopherol, oryzanol and rice germ extract is (2.5-5):(0.2-0.5):(0.1-0.2):(0.25-0.75).

[0014] When the above three synergistic components are combined with sesame seed extract, the optimal synergistic effect between the components can be achieved, resulting in the best antioxidant properties of the prepared fat-soluble composition.

[0015] Preferably, the mass ratio of isosorbide dimethyl ether to hemp seed extract is ≥8:1.

[0016] Experimental studies have revealed that the ratio of isosorbide dimethyl ether to hemp seed extract significantly impacts the stability and efficacy of the final composition. Only when the mass ratio of isosorbide dimethyl ether to hemp seed extract is ≥8:1 can the active ingredient, sesame seed extract, be uniformly dispersed in the system, preventing problems with active ingredient separation due to insufficient solubility and dispersibility. This ensures the stability and effective efficacy of the product, and also guarantees the efficacy studies based on sesame seed extract as a cosmetic ingredient.

[0017] Preferably, the mass ratio of the hemp seed extract to the solvent is (2.5-5):(40-97.4).

[0018] Preferably, the solvent further includes caprylic / capric triglyceride.

[0019] Secondly, the present invention provides the use of the aforementioned fat-soluble composition in the preparation of personal care products.

[0020] Preferably, the product includes cosmetics.

[0021] Thirdly, the present invention provides a method for preparing the aforementioned fat-soluble composition, comprising the following steps: S1. Mix the synergist with the solvent and stir to dissolve at 90-100℃ to obtain solution A; S2. Mix the sesame seed extract with the solvent until homogeneous to obtain solution B; S3. Mix solution A and solution B evenly, filter, and the fat-soluble composition is obtained.

[0022] Fourthly, the present invention provides an anti-wrinkle cosmetic, the cosmetic comprising the aforementioned fat-soluble composition; the fat-soluble composition having a mass percentage content of 0.5-5% in the cosmetic.

[0023] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) This invention combines sesame seed extract with at least two synergists from tocopherol, oryzanol, and rice germ extract, breaking through the antioxidant limitations of single-component or simple mixed systems. The components can form a complementary and synergistic mechanism, which can not only significantly enhance the ability of the composition to scavenge free radicals and inhibit lipid oxidation, but also has an antioxidant effect far superior to the use of sesame seed extract or a single synergist alone. It effectively solves the problem of single efficacy and insufficient intensity of traditional fat-soluble antioxidant components, and provides more comprehensive oxidative protection for fat-soluble systems. (2) The present invention specifically selects a specific amount of isosorbide dimethyl ether as a solvent. Its solubility characteristics can be precisely matched with the physicochemical properties of sesame seed extract and the selected synergist, ensuring that each active ingredient is completely dissolved in the solvent, avoiding precipitation caused by differences in component solubility, and ensuring the uniformity of the composition system. Attached Figure Description

[0024] Figure 1 The graph shows the stability test results of the fat-soluble compositions prepared in Examples 1-9 and Comparative Examples 1-7. Figure 2 A schematic diagram of lipid droplet staining in adipose stem cells using the lipid-soluble composition prepared in Example 1 (10*); PC is the positive group, and BC is the normal group; Figure 3 This is a schematic diagram (40*) of the staining of lipid droplets in adipose stem cells by the lipid-soluble composition prepared in Example 1; PC is the positive group, and BC is the normal group; Figure 4 A before-and-after facial comparison of a subject using the anti-aging face creams D0, D14, and D28 prepared in Example 10. Detailed Implementation

[0025] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0026] Unless otherwise specified, all raw materials used in the following examples and comparative examples are commercially available general-purpose materials.

[0027] Examples 1-9 Examples 1-9 provide a fat-soluble composition, the formulation of which is shown in Table 1. The preparation method of the composition includes the following steps: (1) Dissolve and disperse the synergist in a solvent. Set the water bath temperature to 90-100℃ and stir until completely dissolved and transparent. Set aside as solution A. (2) Under room temperature stirring conditions, sesame seed extract was added to the solvent and stirred until evenly dispersed. This solution is denoted as solution B. (3) Add solution A, which is kept at a constant temperature (temperature controlled at 60-70℃), to solution B, which is stirred, cool down to room temperature, and filter with 400 mesh gauze to obtain the fat-soluble composition.

[0028] Table 1 shows the formulation of the fat-soluble composition (mass percentage). Example 10 This embodiment provides an anti-aging face cream, which includes the fat-soluble composition prepared in Example 2. The formula of the face cream is shown in Table 2.

[0029] Table 2 The preparation process of the anti-aging face cream is as follows: 1. Accurately weigh phase A, heat in an 80℃ water bath, and rapidly homogenize for 3 minutes until uniform; set aside. 2. Accurately weigh phase B, heat and dissolve phase B until it is uniform, add phase B to phase A, stir until uniform, and homogenize for 3-5 minutes; 3. Add the dissolved C phase to neutralize, cool to room temperature, add the D phase and stir well, then discharge.

[0030] The above test formulations, phases A, B, and C, mainly involve commonly used thickeners in cosmetics (sodium hyaluronate, xanthan gum, carbomer), moisturizers (glycerin), neutralizers (arginine), emulsifiers (polyglycerol-10-dipalmitate, sucrose palmitate, cetearyl alcohol), oils (dimethicone, caprylic / capric triglyceride, squalane), preservatives (p-hydroxyacetophenone, 1,2-hexanediol), and chelating agents (sodium gluconate); phase D is the functional component. Based on the efficacy test in Example 2, phase D is the protective phase. Other components can be selected from functional components defined in cosmetics, as long as they do not affect the functionality of the active phase. Therefore, the above test formulations include, but are not limited to, the above components, and any raw materials that comply with cosmetic quality and safety regulations can be used.

[0031] Comparative Examples 1-7 Comparative Examples 1-7 provide a fat-soluble composition, the formulation of which is shown in Table 3. The preparation method of the composition is the same as that of the examples, except for the formulation.

[0032] Table 3 shows the formulation of the fat-soluble compositions (mass percentage). Example 1 This example uses the lipid-soluble compositions prepared in Examples 1-9 and Comparative Examples 1, 5-7 as test samples to test their stability. The specific methods are as follows: The samples were placed in high temperature (40℃) and low temperature (8℃) environments for 30 days to observe whether there was precipitation or sedimentation, in order to test the physicochemical stability of the samples. The specific test results are shown in Table 4.

[0033] Table 4 Table 4 shows that the fat-soluble composition prepared by the technical solution of the present invention has good physicochemical stability. The active ingredients remain uniformly dispersed even after long-term storage under high and low temperature conditions, without any solid precipitation, layering, or sedimentation, thus providing a basic guarantee for the sustained efficacy of the product. However, a comparison between Comparative Example 1 and Example 2 shows that when the mass ratio of isosorbide dimethyl ether to sesame seed extract is less than 8:1, the composition exhibits precipitation of insoluble substances that are insoluble upon heating. In Comparative Examples 5-7, when the synergist is only one of rice germ extract, tocopherol, and oryzanol combined with sesame seed extract and the amount of the component is relatively high, precipitation problems also occur under different test environments, indicating poor stability.

[0034] Example 2 This effect example uses the fat-soluble compositions prepared in Examples 1-9 and Comparative Examples 1-7 as test samples to determine their antioxidant properties. The specific methods are as follows: (1) Linoleic acid peroxidation test: 1. Experimental Principle Ferric thiocyanate method (FTC): The ferric thiocyanate (FTC) colorimetric method is based on the fact that under acidic conditions, peroxides formed by lipid oxidation can detect Fe... 2+ Oxidized to Fe 3+ Then Fe 3+ The absorbance can be measured by forming a red complex with thiocyanate ions, which has maximum absorption in the range of 480-515 nm.

[0035] 2. Testing Methods 2.1 Reagents The water used was laboratory-grade water; Methanol solution: Dilute 80 mL of methanol with water to 100 mL; Linoleic acid solution (2.5%): Linoleic acid (Maclean, content ≥99.0%, CAS: 60-33-3), prepared with anhydrous ethanol; anhydrous ethanol; 75% ethanol; PBS buffer (pH 7.0): Mix 39.0 mL of 0.05 mol / L NaH2PO4 and 61.0 mL of 0.05 mol / L Na2HPO4; 0.05 mol / L Na2HPO4: Weigh 17.911 g of Na2HPO4·12H2O and dissolve it in 1000 mL of water; 0.05 mol / L NaH2PO4 4 Weigh 7.8015g of NaH2PO4·2H2O and dissolve it in 1000mL of water; Ammonium thiocyanate solution (30%): Weigh 30g of ammonium thiocyanate and dissolve it in 100mL of water; Hydrochloric acid solution (3.5%): Measure 8.8 mL of reagent hydrochloric acid and dilute with water to 100 mL; 2.2 Preparation of reference solution Take 2.0 mL of methanol solution, add 2 mL of linoleic acid solution and 4 mL of PBS buffer, and then perform the measurement.

[0036] 2.3 Sample solution preparation Weigh the sample and add methanol to prepare a 10 mg / mL solution, then dilute to obtain a 2.5 mg / mL test sample solution. Take 2 mL of the above sample solution, add 2 mL of linoleic acid solution and 4 mL of PBS buffer, and perform a blank test using methanol solution. During the sample solution preparation process, due to the crystal precipitation problem in the comparative sample, the comparative sample was shaken thoroughly before sampling to ensure relatively accurate experimental data.

[0037] 2.4 Operation The control solution and sample solution were placed in a 40℃ constant temperature incubator for 24h, 72h, and 120h before measurement.

[0038] 2.5 Measurement Take 0.1 mL of the sample solution, add 4.7 mL of 75% ethanol solution, 0.1 mL of 30% ferric thiocyanate solution, and 0.1 mL of ferrous chloride solution, mix well; let stand for 3 min, and measure the absorbance of the mixture at a wavelength of 500 nm.

[0039] 2.6 Calculation Linoleic acid peroxidation inhibition rate (%) = 1 - (OD 样品 / OD 对照 )×100% Table 5 shows the inhibition of linoleic acid peroxide in samples with the same test concentration (0.25 mg / mL) after the same number of test days (1, 3, 5). The results can be used to determine the antioxidant capacity of different fat-soluble compositions.

[0040] Table 5 (2) DPPH clearance rate test: The lipid-soluble compositions prepared in Examples 1-9 and Comparative Examples 1-7 were diluted with 95% ethanol to obtain samples of varying concentrations, according to the different samples. During sample solution preparation, due to crystal precipitation issues in the comparative examples, the comparative examples were shaken thoroughly before sampling to ensure relatively accurate experimental data.

[0041] According to the standard test method of TSHRH006-2018 "Cosmetics - Experimental Method for Free Radical (DPPH) Scavenging", the relationship curve between concentration and DPPH free radical scavenging rate was obtained, and the IC50 value (the sample concentration value corresponding to the free radical half-maximum inhibition rate) was finally obtained. The specific test results are shown in Table 6.

[0042] Table 6 As shown in Tables 5-6, the fat-soluble composition prepared by the present invention using a specific technical solution exhibits excellent antioxidant capacity in inhibiting linoleic acid peroxide and scavenging DPPH free radicals. However, Comparative Examples 2-4 show that when only one of rice germ extract, tocopherol, or oryzanol is used as a single synergist in combination with sesame seed extract, the antioxidant performance of the resulting composition significantly decreases. In contrast, Examples 2 and 5-7, when using two or three of the above synergists in combination with sesame seed extract, show a significant synergistic effect among the components, resulting in a substantial improvement in the antioxidant performance of the composition. The antioxidant effect is optimal, especially when rice germ extract, tocopherol, and oryzanol coexist. Furthermore, even when the amount of a single synergist in Comparative Examples 5-6 is increased to the same total amount as in Example 2, its antioxidant effect is still far inferior to that of Example 2, fully demonstrating the synergistic effect of "1+1>2" between the multiple synergists used in the present invention and sesame seed extract.

[0043] Example 3 This effect example uses the lipid-soluble composition prepared in Example 1 as the test sample. Through functional studies on its promotion of primary adipocyte differentiation, it is used to evaluate its ability to maintain the positive differentiation of adipocytes and avoid skin wrinkles caused by insufficient differentiation, so as to evaluate the anti-wrinkle function of the sample.

[0044] I. Experimental Principle Human primary adipose-derived stem cells (AMSc) are a type of stem cell with multi-directional differentiation potential isolated from adipose tissue. They can be induced to differentiate into adipocytes and are often used in the study of mechanisms related to fat deposition.

[0045] II. Instruments and Equipment Microbalance, clean bench, CO2 incubator, microplate reader, water bath, biological microscope.

[0046] III. Reagents and Materials 3.1 Reagents Cell Counting Kit-8 reagents; mesenchymal stem cell culture system; adipocyte induction differentiation culture system; 0.25% Trypsin-EDTA; Oil Red O staining kit.

[0047] 3.2 Cell lines Human primary adipose-derived stem cells (AMSc).

[0048] IV. Operating Procedures 4.1 Pretreatment of test substance The stock solution from Example 1 was subjected to sterile filtration pretreatment using a 0.22 μm filter, and then diluted to the required concentration using culture medium.

[0049] 4.2 Cytotoxicity Test: After cell counting, the cells were diluted to the required concentration, and the cell suspension was added to 96-well microplates to ensure that the culture medium volume in each well was 100 μL and the cell count was 1 × 10⁶ cells / well. 4 Cells. After 24 hours of plating, the test substance was added using a multi-channel pipette to achieve the set concentration. A solvent control was set up, and at least three parallel test wells were prepared. 48 hours after sample addition, the waste liquid was removed, and medium containing 10% CCK-8 was added. After incubation for 1-1.5 hours, the cells were detected using a microplate reader with an OD of 450 nm.

[0050] 4.3 Cell seeding for drug administration: Dilute cells to the seeding density with cell culture medium, then seed them into 24-well plates and incubate in a CO2 incubator for 24h ± 2h.

[0051] Lipidification induction and sample loading inhibition in cells: The BC control group was set up, and the medium was changed continuously using the mesenchymal stem cell culture system to maintain the original growth state of the cells. The PC control group was set up, and the medium was changed continuously using the adipocyte induction differentiation culture system to induce adipogenesis in human primary adipocyte stem cells, thereby changing their growth state and physiological characteristics and causing them to secrete a large number of lipid droplets. The induction process lasted for two weeks, and the medium was changed every 2-3 days.

[0052] The experimental results, based on the cytotoxicity test procedure in 4.3, are shown in Table 7: Table 7. Cytotoxicity results of Example 1 on human primary adipose stem cells. Concentration (%) Cell viability (%) SD (%) 0.00 100.00 3.46 0.0020 103.21 2.65 0.0039 101.66 3.33 0.0078 103.70 4.32 0.0156 107.09 2.77 0.0313 107.48 6.46 0.0625 110.00 5.01 0.1250 107.96 3.18 0.2500 97.16 5.10 0.5000 83.42 4.07 Based on the results in Table 7, 0.3% of Example 1 was selected as the safe concentration for testing. The differentiation morphology and lipid droplet distribution of human adipocytes were recorded according to the experimental procedures described above. The results are shown in Table 7. Figure 2-3 The image shows that the lipid-soluble composition prepared by the technical solution of the present invention has an inducing effect on lipidization of human primary adipose stem cells, and can effectively protect and repair the facial fat structure, thereby achieving the effects of facial fat differentiation correction and structural anti-aging.

[0053] Example of effect 4 This efficacy example uses the anti-aging face cream prepared in Example 10 as the test sample. The anti-aging efficacy of the sample is evaluated by comparing the face before and after the human trial. After cleansing, the faces of the test volunteers were photographed using the VISIA facial image analyzer. The information obtained from the VISIA facial image analyzer at 0-14-28 days was compared with the information before the test to analyze the improvement of facial wrinkles and pigmentation. The higher the value, the more obvious the pigmentation and texture.

[0054] (1) Target number of test cases: 35 people to be included, and ≥30 people to be completed; Evaluation: Investigator assessment, image acquisition, instrument measurement methods, and self-assessment by subjects after using the product; Test environment: constant environmental conditions with a temperature of 20℃~22℃ and a relative humidity of 40%-60%RH; Testing instrument: VISIA 7 Testing period: 28 days; Time points: D0, D14 and D28 (2) Inclusion and exclusion criteria for volunteers 1. Selection criteria: a) Those aged 30-60.

[0055] b) Trained personnel who have passed the wrinkle level assessment will rate the wrinkles on the left and right outer corners of the eyes of volunteers, with the wrinkles on both sides rated between 1 and 7.

[0056] c) Those with the same level of wrinkles at the outer corners of their eyes on both the left and right sides.

[0057] 2. Exclusion criteria: a) Individuals who have undergone cosmetic surgery or other cosmetic procedures that have affected the appearance of wrinkles at the outer corners of their eyes within the past six months.

[0058] b) Individuals who have used muscle relaxants within the past six months.

[0059] c) Anyone who has used any topical medication on the test site within the past two months.

[0060] d) Individuals who have used a preparation that claims to affect the state of skin wrinkles on the test site within the past month.

[0061] e) Those who have used antihistamines within the past week or immunosuppressants within the past month;

[0062] f) Individuals with inflammatory skin diseases that have not yet healed clinically.

[0063] g) Patients with insulin-dependent diabetes mellitus.

[0064] h) Patients with asthma or other chronic respiratory diseases who are currently receiving treatment; i) Patients who have received anti-cancer chemotherapy within the past six months.

[0065] j) Patients with immunodeficiency or autoimmune diseases.

[0066] k) Lactating or pregnant women.

[0067] l) Those who have undergone bilateral mastectomy and bilateral axillary lymph node dissection.

[0068] m) Those in whom the test result determination is affected due to scars, pigmentation, atrophy, nevus flammeus, or other defects at the skin test site.

[0069] n) Those who participate in other clinical trials.

[0070] o) Those with highly sensitive constitutions.

[0071] p) Non-voluntary participants or those who cannot complete the specified content according to the test requirements.

[0072] After data collection and analysis, the specific pre- and post-use change rates are shown in Table 8-9. The facial comparison diagrams of the subjects using the product at D0, D14, and D28 are shown in Figure 4 .

[0073] Table 8 Change Rates of Freckles Before and After Use Table 9 Change Rates of Wrinkles Before and After Use Note: P>0.05 indicates no significant difference; 0.01<P<0.05 indicates significant difference; P<0.01 indicates extremely significant difference. Experimental data show that the total number of freckles, the number of brown freckles, and the number of wrinkles on the subjects' faces have all significantly decreased, proving that the 2% liposoluble composition can significantly improve the skin condition and reduce the deposition of lipofuscin, and has a significant anti-aging effect.

[0074] Finally, it should be noted that the above embodiments are used to illustrate the technical solutions of the present invention rather than limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A fat-soluble composition, characterized in that, The composition comprises sesame seed extract, a synergist, and a solvent, wherein the synergist is at least two of tocopherol, oryzanol, and rice germ extract; and the solvent comprises isosorbide dimethyl ether.

2. The fat-soluble composition according to claim 1, characterized in that, Includes at least one of the following (1)-(4): (1) The synergist is tocopherol and oryzanol, and the mass ratio of sesame seed extract to tocopherol and oryzanol is (2.5-5):(0.2-0.5):(0.1-0.2); (2) The synergist is tocopherol and rice germ extract, and the mass ratio of sesame seed extract to tocopherol and rice germ extract is (2.5-5):(0.2-0.5):(0.25-0.75); (3) The synergist is oryzanol and rice germ extract, and the mass ratio of sesame seed extract to oryzanol and rice germ extract is (2.5-5):(0.1-0.2):(0.25-0.75); (4) The synergist is tocopherol, oryzanol and rice germ extract, and the mass ratio of sesame seed extract to tocopherol, oryzanol and rice germ extract is (2.5-5):(0.2-0.5):(0.1-0.2):(0.25-0.75).

3. The fat-soluble composition according to claim 2, characterized in that, The synergist is tocopherol, oryzanol and rice germ extract, and the mass ratio of sesame seed extract to tocopherol, oryzanol and rice germ extract is (2.5-5):(0.2-0.5):(0.1-0.2):(0.25-0.75).

4. The fat-soluble composition according to claim 1, characterized in that, The mass ratio of isosorbide dimethyl ether to hemp seed extract is ≥8:

1.

5. The fat-soluble composition according to claim 1, characterized in that, The mass ratio of the hemp seed extract to the solvent is (2.5-5):(40-97.4).

6. The fat-soluble composition according to claim 1, characterized in that, The solvent also includes caprylic / capric triglyceride.

7. The use of the fat-soluble composition according to any one of claims 1-6 in the preparation of personal care products.

8. The method for preparing the fat-soluble composition according to any one of claims 1-6, characterized in that, Includes the following steps: S1. Mix the synergist with the solvent and stir to dissolve at 90-100℃ to obtain solution A; S2. Mix the sesame seed extract with the solvent until homogeneous to obtain solution B; S3. Mix solution A and solution B evenly, filter, and the fat-soluble composition is obtained.

9. An anti-wrinkle cosmetic, characterized in that, The cosmetic product comprises the fat-soluble composition according to any one of claims 1-6; the fat-soluble composition is present in the cosmetic product at a mass percentage of 0.5-5%.

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