Method for repairing and maintaining ecological bank protection structure of water conservancy project

Abstract

The invention provides a method for repairing and maintaining an ecological bank protection structure of a water conservancy project. Comprising the following steps: S1, screening and domesticating functional microorganisms; S2, constructing a vegetation and microorganism synergistic remediation unit; S3, precisely arranging the synergistic remediation unit on site; S4, dynamically monitoring and coordinately regulating and controlling: screening and domesticating the functional microorganisms, constructing the synergistic remediation unit, precisely arranging the synergistic remediation unit on site, and dynamically monitoring and regulating and controlling. A microorganism-vegetation-carrier synergistic effect mechanism is formed, so that the technical problems of structural stability and ecological function separation in traditional revetment restoration are solved; the method has the advantages that the synergistic interaction of structure stability and ecological restoration is realized, the self-repairing capability of a microorganism-vegetation symbiotic system is improved, a multi-dimensional dynamic monitoring regulation and control mechanism is established, and the manual maintenance cost is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of ecological restoration of water conservancy projects, and in particular to a method for repairing and maintaining an ecological revetment structure of a water conservancy project. BACKGROUND

[0002] As a key structure for maintaining the flood control safety and ecological system health of a river, the ecological revetment of a water conservancy project needs to meet the dual requirements of scouring resistance and ecological function restoration. The traditional revetment restoration technology has obvious technical fragmentation: in terms of structural restoration, a single rigid or flexible reinforcement method is generally used, such as gabion baskets or concrete retaining walls, etc. Although this method can improve the scouring resistance of the revetment in the short term, it is prone to problems such as structural cracking and destruction of biological habitats in the long term, and it is difficult to achieve the unity of structural durability and ecological compatibility. The ecological maintenance aspect is mostly limited to the level of surface vegetation reseeding, and lacks systematic regulation of deep ecological elements such as soil microbial communities and hydrological connectivity, resulting in low vegetation survival rate and slow ecological function recovery.

[0003] The monitoring system in the prior art has serious defects: the structural monitoring only focuses on physical parameters such as slope displacement and settlement, and the ecological monitoring is limited to scattered vegetation coverage statistics, the two types of data collection systems are independent of each other, and cannot form an effective collaborative analysis mechanism. The data processing link relies too much on manual experience, lacks an intelligent evaluation model that can associate structural deformation and ecological degradation in real time, and the restoration decision is lagging behind the actual engineering needs. For example, when the microbial population of the revetment decreases sharply, the traditional method is difficult to find and take timely intervention measures, often delaying the best repair opportunity.

[0004] The lack of system integration is another prominent defect: the compatibility of the structural restoration components and the ecological monitoring equipment is poor, and the adaptability in different water level zones and geological conditions is weak. The restoration unit in the high water level area is prone to failure due to water flow scouring, and in the low water level area, the microorganisms are often inactivated due to drought, which causes regional functional imbalance and forces the operation and maintenance personnel to frequently implement manual intervention, greatly increasing the management cost. In addition, the microbial-vegetation coordination mechanism is missing in the prior art, and the functional bacteria and plant roots do not form a symbiotic system, resulting in short-term soil improvement effect and repeated degradation of the revetment ecological system.

[0005] In view of the above problems, the prior art needs to be improved. SUMMARY

[0006] Therefore, the embodiments of the present application aim to provide a method for repairing and maintaining an ecological revetment structure of a water conservancy project to solve or alleviate the technical problems in the prior art, and at least provide a beneficial choice.

[0007] To solve the above technical problems, one technical scheme adopted by the present application is to provide a water conservancy project ecological revetment structure repair and maintenance method, comprising the following steps:

[0008] S1. Screening and domestication of functional microorganisms: Collect samples from healthy soil and rhizosphere environment of the target revetment area, use selective medium to separate and purify microorganisms in the samples, and screen to obtain strains with functions of nitrogen fixation, phosphorus solubilization, growth promotion, and soil aggregate enhancement; place the strains in a culture medium simulating the revetment environment for domestication culture, and adjust the environmental factors by gradient to make the survival rate of the strains in the target revetment environment meet the preset requirements.

[0009] S2. Construction of vegetation and microorganism cooperative repair unit: According to the vegetation type corresponding to the water level partition of the revetment, the functional microorganisms domesticated in step S1 are specifically matched with the vegetation; using ecological concrete prefabricated blocks containing slow-release nutrient matrix as carriers, the functional microbial agents, vegetation seeds, water retaining agents, and organic fertilizers are mixed in proportion and filled into the pores of the prefabricated blocks to form a microbial, vegetation, and carrier integrated repair unit, wherein the water permeability of the carrier meets the preset water permeability requirement.

[0010] S3. On-site precise layout of the cooperative repair unit: According to the revetment structure stability evaluation results, the cooperative repair unit is laid out in the slope collapse risk area, the vegetation degradation serious area, and the strong hydraulic scouring area; according to different water level partitions, the corresponding layout method is adopted, and when laying out, the depth of the prefabricated blocks embedded in the soil body meets the preset depth requirement, and is fixed through the fixing piece; a water permeable geotextile is laid within the preset range around the unit.

[0011] S4. Dynamic monitoring and cooperative regulation: A microbial, vegetation, and environmental multi-parameter monitoring system is constructed, and microbial activity sensors, vegetation growth sensors, and environmental factor sensors are laid out in the repair unit area; soil samples are collected regularly, and the change of the number of functional microbial populations is detected, and when the number of the populations is lower than the preset threshold, the corresponding functional microbial agent is supplemented; according to the vegetation growth monitoring data, when the vegetation coverage is lower than the preset coverage, the types and amounts of microbial agents are adjusted in combination with the soil nutrient detection results.

[0012] As a further preferred technical solution of the present application, in step S1, the selective medium includes Azotobacter medium for nitrogen-fixing bacteria separation and Monkhina medium for phosphorus-solubilizing bacteria separation.

[0013] As a further preferred technical solution of the present application, in step S1, the environmental factors simulating the revetment environment include temperature, pH value, and salinity, and the gradient adjustment is to gradually approach the target revetment actual environmental factor range at a preset interval.

[0014] As the further preferred of the technical solution: in step S2, the water level partition includes high water level area, medium water level area and low water level area; the vegetation of the high water level area is Salix pedunculiflora, Salix matsudana, and the nitrogen-fixing bacteria and cellulose-decomposing bacteria with water tolerance are matched; the vegetation of the medium water level area is Phragmites australis, Acorus calamus, and the phosphorus-decomposing bacteria and rhizosphere microorganism promoting bacteria are matched; the vegetation of the low water level area is Cynodon dactylon, Iris laevigata, and the microorganism group with drought resistance and soil improvement function is matched.

[0015] As the further preferred of the technical solution: in step S2, the concentration of the functional microorganism agent meets the preset concentration requirement, and the porosity of the prefabricated block meets the preset porosity requirement.

[0016] As the further preferred of the technical solution: in step S3, the corresponding arrangement mode includes: the high water level area adopts row-column arrangement, the medium water level area adopts plum blossom arrangement, and the low water level area is randomly arranged in combination with the pebble beach terrain; the preset depth requirement is that the embedding depth in the soil body is not less than 20 cm, and the preset range is within 50 cm of the unit periphery.

[0017] As the further preferred of the technical solution: in step S4, the microorganism activity sensor is used for monitoring the activities of dehydrogenase and urease; the vegetation growth sensor is used for monitoring the plant height and leaf area index; and the environmental factor sensor is used for monitoring the soil water content, pH value and pore water pressure.

[0018] As the further preferred of the technical solution: in step S4, the preset threshold value is that the number of the functional microorganism population is less than 10 6 CFU / g, and the preset coverage rate is that the vegetation coverage is less than 60%.

[0019] To solve the above technical problems, another technical solution adopted by the present application is: a water conservancy project ecological revetment structure repair and maintenance system, comprising a structure repair module, an ecological monitoring module and a data processing module;

[0020] The structure repair module comprises a flexible structure unit for slope reinforcement, a rigid and flexible combined unit and a foundation protection unit, the flexible structure unit is provided with an ecological bag and geogrid connection assembly, the rigid and flexible combined unit is provided with a crack sealing member and a vegetation concrete spray seeding assembly, and the foundation protection unit comprises a scour protection cushion layer and a filter layer.

[0021] The ecological monitoring module is arranged in different water level partitions of the revetment and comprises a vegetation growth monitoring assembly, a soil microorganism monitoring assembly and a hydrological environment monitoring assembly.

[0022] The data processing module is in communication connection with the structure repair module and the ecological monitoring module respectively, and is used for receiving monitoring data and generating repair and maintenance instructions.

[0023] As the further preferred technical solution of the present application, the ecological monitoring module further comprises a distributed sensing assembly, which is embedded in the revetment soil body and structure, and is used for collecting strain, water content and pore water pressure data.

[0024] The data processing module is internally provided with a structure stability evaluation model and an ecological function evaluation model, which can output structure reinforcement suggestions and ecological regulation schemes according to the distributed sensing assembly and the vegetation and microbial monitoring data.

[0025] To solve the above technical problems, another technical solution adopted by the present application is a computer device, which comprises a processor and a memory coupled with the processor, and the memory stores program instructions, and the program instructions are executed by the processor to make the processor execute the steps of the water conservancy ecological revetment structure repair and maintenance method.

[0026] To solve the above technical problems, another technical solution adopted by the present application is a storage medium storing program instructions capable of realizing the water conservancy ecological revetment structure repair and maintenance method.

[0027] As can be seen from the above, the water conservancy ecological revetment structure repair and maintenance method and system provided by the present application form a microbial-vegetation-carrier synergistic mechanism through functional microbial screening and domestication, collaborative repair unit construction, on-site precise layout and dynamic monitoring and regulation, which solves the technical problem of the separation of structure stability and ecological function in traditional revetment repair, has the advantages of realizing synergistic effect of structure stability and ecological repair, improving self-repairing ability of microbial-vegetation symbiotic system, establishing multi-dimensional dynamic monitoring and regulation mechanism, and reducing artificial maintenance cost.

[0028] The above summary is only for the purpose of the description and is not intended to limit in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the present application will be apparent from the drawings and the following detailed description. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can obtain other drawings according to these drawings without any creative effort.

[0030] Figure 1 The flowchart of the method of the present application;

[0031] Figure 2A schematic diagram of a module of the system of the present application. DETAILED DESCRIPTION

[0032] The technical solutions in the present application will be described clearly and completely below in combination with the drawings in the present application. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. The components of the present application described and shown in the drawings herein can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative work belong to the scope of protection of the present application. It should be noted that: similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings. Meanwhile, in the description of the present application, the terms “first”, “second”, etc. are only used for differentiation in description, and cannot be understood as indicating or implying relative importance.

[0033] In the prior art, the repair and maintenance of the ecological revetment of water conservancy projects often faces the problem that the structural stability and ecological function are difficult to be coordinated. The traditional method uses rigid or flexible structure for single reinforcement, such as gabion or concrete retaining wall, which can short-term improve the anti-erosion ability, but hinders the material exchange between soil and water, resulting in difficulty in natural recovery of vegetation. Ecological maintenance measures are mostly limited to surface vegetation reseeding, lacking of systematic regulation of soil microbial community and hydrological conditions, resulting in short-term repair effect. In a certain river revetment engineering, long-term hydraulic scouring leads to slope collapse, and soil hardening causes vegetation degradation, and the conventional repair method cannot realize the synchronous improvement of structural stability and ecological restoration.

[0034] In order to solve the above problems, it is necessary to break through the inherent mode of separation of structural repair and ecological regulation. Through analysis, it is found that microbial community plays a key role in soil aggregate formation and nutrient cycling, and the prior art does not include it in the revetment repair system. Further thinking finds that if functional microorganisms and revetment vegetation can be configured coordinately, and carrier units suitable for different water level environments can be constructed, the structural stability and ecological function can be simultaneously enhanced. At the same time, the lack of dynamic monitoring system leads to lagging repair measures, and if real-time microbial activity and vegetation growth data can be obtained, a precise regulation mechanism can be established. Based on this, the technical idea of combining the microbial-vegetation-carrier integrated repair unit and the multi-parameter monitoring system is formed.

[0035] As Figure 1Therefore, the application proposes a water conservancy project ecological revetment structure repair and maintenance method, which comprises the following steps: collecting soil and rhizosphere samples from the target revetment area, screening microbial strains with nitrogen fixation and phosphorus solubilization functions and performing environmental domestication; matching vegetation and microbial types according to water level zoning, filling microbial agents, seeds and water retaining agents into the pores of the ecological concrete precast blocks to form a repair unit; embedding the repair unit in the soil and reinforcing it through a fixing member according to the results of slope stability evaluation; laying microbial activity, vegetation growth and environmental factor sensors, and dynamically adjusting the microbial agent supplement strategy according to the monitoring data.

[0036] Among them, the screening and domestication of functional microorganisms refers to the separation of target strains through selective culture medium, and gradually adjusting parameters such as temperature and pH value in a simulated revetment environment, so that the strain adapts to the actual working condition. This step ensures the survival ability of microorganisms in complex environment, and provides activity guarantee for subsequent repair. The construction of vegetation and microbial collaborative repair unit refers to the combination of domesticated strains and water level zoning adapted vegetation, and the use of the pore structure of ecological concrete precast blocks to carry the substances required for microbial and plant growth. The carrier design takes into account the water permeability and structural strength, promotes root development and microbial colonization. Precise layout on site refers to the use of differentiated layout mode in different water level areas according to the evaluation results of slope collapse risk, vegetation degradation degree, etc. By controlling the embedding depth of precast blocks and the laying range of surrounding water permeable geotextile, the interaction between repair unit and soil is optimized. Dynamic monitoring and collaborative regulation refers to real-time acquisition of microbial activity, vegetation growth index and soil environment parameters through sensor network, and triggering of microbial agent supplement mechanism when the monitoring value is lower than the preset threshold. This step forms a closed-loop control system to maintain the long-term stability of the repair effect.

[0037] Specifically, first, the directional screening and adaptive training of functional microorganisms are completed in a laboratory environment to ensure their metabolic activity in actual revetment soil. Then, the domesticated strains and vegetation seeds are combined and packaged according to the water level zoning characteristics, and the slow-release function of ecological concrete precast blocks is used to continuously supply nutrients. In the engineering implementation stage, based on the results of slope stability analysis, embedded layout mode is used in high scouring areas to enhance the structural stability through mechanical engagement of precast blocks and soil. After the repair unit is laid out, the number of microbial populations and the growth state of vegetation are monitored in real time through a sensor network, and when abnormal key indicators are detected, microbial agent supplement or nutrient adjustment instructions are automatically triggered to realize dynamic optimization of the repair process.

[0038] Compared with the prior art, the application of microorganisms in the traditional revetment repair method is limited to laboratory research, and no engineering implementation scheme is formed. The method realizes the engineering integration of microorganisms-vegetation through the precast block carrier, solves the problems of uneven field application of microbial agents and low survival rate. The existing monitoring system mostly adopts independent collection mode, and the scheme establishes a correlation evaluation model of structural stability and ecological function through multi-sensor data fusion, which significantly improves the timeliness of regulation and control. In addition, the traditional rigid repair structure blocks the ecological connectivity, while the combination design of the water permeable precast block and the geotextile in the scheme enhances the anti-scouring ability while maintaining the water-soil exchange channel.

[0039] Through the above technical scheme, the application realizes the synergistic effect of revetment structure repair and ecological function recovery. The three-dimensional synergistic mechanism of microorganisms-vegetation-carrier effectively enhances the stability of soil aggregates and reduces the risk of slope collapse. The multi-parameter monitoring system optimizes the regulation and control strategy through real-time data feedback, reducing the frequency of manual maintenance. The repair unit layout mode adapted to the water level partition solves the problem of poor adaptability of traditional methods under different hydrological conditions. The combination application of water permeable carrier and fixing part improves the durability of the structure while promoting the natural succession of the vegetation community.

[0040] The application further proposes that in step S1, the selective medium includes Azotobacter medium for isolating nitrogen-fixing bacteria and Monchyna medium for isolating phosphorus-solubilizing bacteria.

[0041] The Azotobacter medium refers to a medium without nitrogen source, which can be prepared by using mannitol, phosphate, sulfate and trace elements. By limiting nitrogen supply, microorganisms are forced to fix atmospheric nitrogen, thereby screening strains with nitrogen-fixing ability. The Monchyna medium refers to a medium containing insoluble phosphate, which can use tricalcium phosphate or iron phosphate as the only phosphorus source. By detecting the change of soluble phosphorus content in the medium, strains with phosphorus-solubilizing ability can be screened. The above two media can selectively screen target functional bacteria by limiting specific components, avoiding interference of non-functional microorganisms.

[0042] Specifically, in the process of microorganism screening, soil samples collected from the revetment area are inoculated in Azotobacter medium and Monchyna medium for enrichment culture. In the Azotobacter medium, only nitrogen-fixing bacteria that can utilize atmospheric nitrogen can grow normally, while other microorganisms that rely on external nitrogen are inhibited due to nutrient deficiency; in the Monchyna medium, phosphorus-solubilizing bacteria that can decompose insoluble phosphorus dissolve phosphate by secreting organic acids, forming a transparent circle around the colony. By observing the colony morphology and transparent circle size, high-activity nitrogen-fixing bacteria and phosphorus-solubilizing bacteria can be quickly identified and isolated, providing high-quality strain resources for subsequent domestication.

[0043] Compared with the prior art, the conventional microbial screening is mostly carried out by using a general culture medium, which cannot effectively distinguish functional bacteria from non-functional bacteria, resulting in low screening efficiency and mixed functions of the strains. Through the design of a targeted culture medium, the present application realizes directional screening of functional bacteria in the isolation stage, reduces the subsequent domestication workload, and at the same time ensures the accurate matching of the functions of the strains and the needs of bank protection repair.

[0044] Through the above technical solution, the present application solves the problem of insufficient targeting of the conventional microbial screening method, significantly improves the isolation efficiency of nitrogen-fixing bacteria and phosphorus-dissolving bacteria, lays a strain foundation for constructing an efficient microbial-vegetation collaborative repair system, and thus enhances the soil nutrient supply capacity and ecological stability in the bank protection structure repair process.

[0045] The present application further proposes that the environmental factors simulating the bank protection environment in step S1 include temperature, pH value and salinity, and the gradient adjustment is to gradually approach the target bank protection actual environmental factor range at a preset interval.

[0046] Among them, the environmental factor refers to a key physicochemical parameter affecting the survival and function expression of microorganisms, which can be realized by using a temperature controller, a pH buffer and a salinity regulator. The temperature control range can be 5-40℃, the pH adjustment range can be 6.0-8.5, and the salinity adjustment range can be 0.1-3.0%. Gradient adjustment refers to the operation mode of changing environmental parameters in stages, which can be realized by adjusting the temperature by ±2℃, the pH by ±0.5 and the salinity by ±0.2% per day, so that the microorganisms gradually adapt to the target environmental conditions.

[0047] Specifically, in the microbial domestication process, the strains are placed in an incubator containing temperature, pH value and salinity control devices for domestication culture. The initial value of the culture environment parameters is set as the standard laboratory condition, and then gradually adjusted to the target bank protection measured parameter range at a preset interval. For example, when the target bank protection actual temperature is 25℃, the culture temperature can start from 20℃ and increase by 1℃ per day, and reach the target temperature after five days. In this process, the metabolic activity of the microorganisms is continuously monitored, and when the survival rate is less than 85%, the gradient interval is adjusted to a smaller amplitude. Through this progressive adaptation mechanism, the stability of the cell membrane structure of the microorganisms is maintained, and the key enzyme activity is maintained in the effective working interval.

[0048] Compared with the prior art, the conventional microbial domestication method directly exposes the strains to target environmental conditions, resulting in more than 60% of the strains being inactivated due to environmental mutation. However, through the stage-by-stage parameter approximation of the present application, the microorganisms complete adaptive gene expression within the metabolic regulation period, the survival rate is increased to more than 90%, and the retention rate of the nitrogenase activity of the functional strains is increased by about 40%.

[0049] By the technical scheme, the application effectively solves the technical problem of low survival rate of microorganisms in the actual environment of the revetment, enables the functional strains to continuously play a role in the ecological restoration process of soil aggregate formation and nutrient transformation, and guarantees the long-term stability of the revetment structure restoration.

[0050] The application further proposes that the water level zoning includes a high water level zone, a medium water level zone and a low water level zone; the vegetation in the high water level zone is Salix integra and Salix matsudana, and is matched with water-tolerant nitrogen-fixing bacteria and cellulose-decomposing bacteria; the vegetation in the medium water level zone is Phragmites australis and Acorus calamus, and is matched with phosphorus-decomposing bacteria and rhizosphere microorganisms; and the vegetation in the low water level zone is Cynodon dactylon and Iris laevigata, and is matched with microorganism groups with drought resistance and soil improvement functions.

[0051] The water level zoning refers to a vertical space zone divided according to the water level fluctuation characteristics corresponding to different elevations of the revetment area, and the boundary range can be determined by elevation measurement combined with historical hydrological data, and is used to adapt to the ecological restoration requirements under different water level conditions. The water-tolerant nitrogen-fixing bacteria refer to microbial strains that can maintain metabolic activity in soil with saturated water content, and specific nitrogen-fixing strains isolated from wetland environments can be used for screening. The extracellular polysaccharides secreted by the nitrogen-fixing bacteria can enhance the soil erosion resistance. The cellulose-decomposing bacteria refer to microorganisms that can decompose cellulose components in plant residues, and specific Trichoderma strains can be used to promote nutrient cycling by decomposing litter. The drought-resistant microorganism groups refer to complex microbial systems with drought-resistant metabolic pathways, and specific mixed microbial agents of Bacillus and Actinomyces can be used to improve the drought resistance of vegetation by secreting plant growth-promoting hormones.

[0052] Specifically, the revetment area is divided into three water level zones, and each water level zone is configured with a specific combination of vegetation and functional microorganisms. The high water level zone is in a long-term submerged state, and the flood-tolerant plants Salix integra and Salix matsudana are selected, and are matched with water-tolerant nitrogen-fixing bacteria and cellulose-decomposing bacteria. The former replenishes soil nitrogen through nitrogen fixation, and the latter accelerates the decomposition of plant residues to form organic matter. The medium water level zone is periodically dry and wet, and emergent plants such as Phragmites australis and Acorus calamus are selected, and are matched with phosphorus-decomposing bacteria to release insoluble phosphorus elements in the soil, and rhizosphere microorganisms promote plant root development by secreting organic acids. The low water level zone is significantly affected by drought, and drought-resistant plants Cynodon dactylon and Iris laevigata are selected, and are matched with extracellular polysaccharides secreted by drought-resistant microbial groups to improve soil water-holding capacity, and at the same time, the soil aggregate stability is enhanced through the mycelial network.

[0053] Compared with the prior art, the microbial agent in the traditional revetment repair adopts a single formula, and the habitat difference caused by water level zoning is not considered, resulting in low survival rate of the microbial flora and limited functional expression. By matching the microorganisms and vegetation in different zones, the metabolic characteristics of the microbial flora are adapted to the regional hydrological conditions. For example, cellulose-decomposing bacteria in the high-water-level zone can effectively degrade plant residues in the submerged zone, avoiding the accumulation of organic matter caused by the inactivation of the microbial flora due to lack of oxygen in the traditional method, and water-tolerant nitrogen-fixing bacteria continuously supplement nitrogen sources, overcoming the limitation of vegetation growth caused by nitrogen loss in conventional repair.

[0054] Through the above technical solutions, the present application realizes the functional synergistic optimization of microorganisms and vegetation in different water level zones. The combination of nitrogen-fixing bacteria and cellulose-decomposing bacteria in the high-water-level zone effectively maintains the nutrient cycling efficiency in the submerged environment, the combination of phosphorus-decomposing bacteria and rhizosphere-promoting microorganisms in the medium-water-level zone synergistically enhances the adaptability of plants to intermittent waterlogging, and the drought-resistant microbial flora in the low-water-level zone reduces vegetation degradation caused by drought by improving soil structure. This zoned adaptation mechanism solves the problem of degradation of repair effect caused by mismatch between microbial function and regional environment in the traditional method, and enhances the overall stability of the revetment structure through the synergistic effect of bacteria-plant.

[0055] The present application further proposes that, when constructing the vegetation and microbial synergistic repair unit, the concentration of the functional microbial agent meets the preset concentration requirement, and the porosity of the ecological concrete precast block meets the preset porosity requirement.

[0056] The concentration of the functional microbial agent refers to the order of magnitude of active microorganisms per unit volume of mixed material, which can be determined by spectrophotometry or plate colony counting method, and the preset concentration requirement needs to be dynamically adjusted according to the soil physical and chemical properties of the target revetment area and the fertilizer characteristics required by the vegetation. The porosity of the ecological concrete precast block refers to the ratio of the volume of connected pores in the material to the total volume, which can be realized by adjusting the aggregate gradation or adding pore-forming agents. For example, when using crushed stone with a particle size of 5-10 mm as coarse aggregate, the porosity can be controlled within the range of 20%-35%, and the preset porosity requirement needs to consider the water permeability and structural bearing capacity.

[0057] Specifically, when preparing the repair unit, the concentration of the functional microbial agent needs to be adapted according to the soil nutrient content of the target water level zone. For example, in the low-water-level drought area, the concentration of the microbial agent can be set to 10 8 CFU / g to cope with water stress; in the medium-water-level zone, it is adjusted to 10 7CFU / g to adapt to the periodic wetting and drying environment. The porosity of the ecological concrete precast block needs to be coordinated with the concentration of the microbial agent: when the porosity is increased to more than 30%, the concentration of the microbial agent needs to be increased synchronously to compensate for the risk of nutrient loss caused by the increase in porosity. Through the matching relationship between the preset concentration and the porosity, the colonization space of microorganisms in the carrier is ensured, and the continuous supply capacity of the slow-release nutrient substrate is maintained.

[0058] Compared with the prior art, the traditional repair unit is prepared by adopting a fixed microbial agent concentration or a random porosity design, for example, a commercial microbial agent is directly used without considering regional differences, or a porous material is only selected empirically without quantifying the porosity parameter. This leads to the problems that the concentration of microorganisms in the carrier is too high to cause metabolic inhibition, or the porosity is insufficient to hinder the penetration of root systems. The present scheme realizes the dual control of maintaining the activity of microorganisms and optimizing the function of the carrier by establishing preset standards for the concentration and porosity.

[0059] Through the above technical scheme, the present application solves the technical problems of low microbial colonization efficiency and imbalance between water permeability and structural strength of the carrier in the traditional repair unit, so that the functional microorganisms form a stable biofilm structure in the pores of the precast block, while ensuring the reasonable transmission of water and nutrients in the carrier, significantly improving the synergistic repair efficiency of vegetation and microorganisms, and prolonging the self-maintenance period of the revetment structure.

[0060] The present application further proposes that, in the process of repairing and maintaining the revetment structure, different water level zones are arranged with different repair units, the high water level zone is arranged in a row-column mode, the medium water level zone is arranged in a plum blossom mode, and the low water level zone is randomly arranged in combination with the pebble beach terrain, the embedding depth of the repair unit in the soil is not less than 20 cm, and a water-permeable geotextile is laid within a range of 50 cm around the unit.

[0061] The row-column mode refers to a mode in which the repair units are arranged according to the straight line row and column rule, which can be realized by using a theodolite to position the line, and this mode is suitable for the high water level zone with stable water flow direction and can form a continuous protection belt. The plum blossom mode refers to a hexagonal grid layout in which adjacent units are staggered, which can be realized by dividing the construction area through a triangular grid, and this layout can effectively disperse the hydraulic impact in the medium water level zone. The random arrangement refers to the free adjustment of the unit position according to the concave-convex form of the pebble beach surface, which can be determined by combining a three-dimensional modeling of an unmanned aerial vehicle, and this mode is suitable for the complex terrain characteristics of the low water level zone. The embedding depth is realized by providing a barb structure at the bottom of the precast block, which forms a mechanical anchoring effect after being inserted into the soil, thereby ensuring the position stability of the unit under water flow scouring. The water-permeable geotextile is prepared by using a polyester fiber needle punching process, and a U-shaped nail is used to fix the edge during laying to prevent soil particles from being lost while maintaining the water permeation channel.

[0062] Specifically, in the high water level area, the units are arranged along the revetment contour line, the row spacing can be set to 80 cm, and the column spacing is set to 100 cm, forming a longitudinal continuous protective barrier. In the medium water level area, the center point of each unit and the adjacent six units form a regular hexagon, and the unit spacing is set to 60 cm, which enhances the anti-scouring ability through staggered distribution. In the low water level area, first, the terrain of the gravel beach is scanned to identify the scour pits and protruding stone areas, and the units are densely arranged at the edge of the scour pits and the density of the units is reduced in the protruding areas. When the unit is embedded, a hydraulic impact hammer is used to vertically press the prefabricated block into the soil until the barb structure is completely submerged in the soil layer. After the permeable geotextile is laid, the joints are overlapped by 15 cm and bonded with biodegradable tape to ensure the continuity of the coverage.

[0063] Compared with the prior art, the traditional revetment repair unit is mostly arranged in a single rectangular array, without considering the water power difference of water level zoning, which leads to the fact that the unit in the high water level area is easily destroyed by concentrated water flow, and the unit in the low water level area is not enough to fit the terrain. The present scheme differentiates the arrangement in different zones, forms a continuous anti-scouring structure in the high water level area, disperses the water flow energy in the medium water level area, and fits the natural terrain in the low water level area, which cooperates with the specific embedding depth and the permeable geotextile to realize the synergistic improvement of structural stability and ecological permeability.

[0064] Through the above technical scheme, the present application solves the problem that the existing revetment repair unit arrangement method does not match the water power condition of water level zoning, effectively reduces the unit displacement risk, and reduces the phenomenon of local scouring intensification caused by improper arrangement. At the same time, through the cooperative laying of the permeable geotextile, the ecological exchange function of the soil around the repair unit is maintained.

[0065] The present application further proposes that in the dynamic monitoring and synergistic regulation steps, the microbial activity sensor is used to monitor the dehydrogenase and urease activity; the vegetation growth sensor is used to monitor the plant height and leaf area index; and the environmental factor sensor is used to monitor the soil water content, pH value and pore water pressure.

[0066] The dehydrogenase activity refers to the catalytic ability of the redox reaction in the microbial metabolic process, and can be detected by colorimetry or fluorescence to realize the catalytic substrate of dehydrogenase to produce color-developing substances or fluorescence signals, and is used to reflect the metabolic activity level of the microbial community. The urease activity refers to the ability of microorganisms to decompose urea to produce ammonia, and can be detected by conductivity or indigo blue to realize the concentration change of urea decomposition products, and is used to characterize the nitrogen cycle efficiency. The plant height refers to the vertical height from the base of the plant stem to the top, and can be non-contact measured by a laser ranging sensor or image recognition technology, and is used to reflect the growth habit of the vegetation. The leaf area index refers to the ratio of the total area of the vegetation leaves to the ground surface area per unit of ground surface area, and can be optically measured by a multispectral imager or a leaf area scanner, and is used to evaluate the photosynthetic capacity of the vegetation. The pore water pressure refers to the pressure of water in the soil pores, and can be measured by a osmometer or a tensiometer by measuring the water potential equilibrium state, and is used to analyze the influence of soil water migration on the stability of the revetment structure.

[0067] Specifically, a plurality of types of sensors are arranged in the revetment repair unit area to form a three-dimensional monitoring network. The microbial activity sensor detects the catalytic product concentration of dehydrogenase and urease to real-time feedback the metabolic intensity of the microbial community and the nitrogen conversion efficiency. The vegetation growth sensor collects the plant height and leaf area index data to dynamically track the vegetation biomass accumulation and photosynthetic efficiency. The environmental factor sensor monitors the soil moisture content, pH value and pore water pressure to synchronously obtain the soil water, acid-base environment and seepage state parameters. The above data are collected by a wireless transmission module to a data processing module to establish a correlation model of microbial activity, vegetation growth and environmental factors. When the dehydrogenase activity decreases accompanied by abnormal increase of the pore water pressure, it is determined that the microbial metabolism is affected by water stress, and a bacteria supplement instruction is triggered.

[0068] Compared with the prior art, the traditional method only monitors a single vegetation coverage or soil moisture index, and cannot associate the coupling relationship between microbial activity and soil hydrological parameters. For example, the existing sensor usually only measures the soil moisture content, but does not synchronously obtain the pore water pressure data, so that it is not possible to distinguish the influence of effective water and gravity water on the microorganisms. The present scheme can identify whether the decrease of microbial activity is due to water shortage or abnormal seepage pressure through multi-parameter synchronous monitoring, so as to accurately guide the bacteria supplement strategy.

[0069] By the technical solution, the application can synchronously capture dynamic change data of microbial metabolism, vegetation growth and soil environment, establish a multi-parameter correlation analysis model, and solve the lagging problem of regulation caused by single monitoring dimension. Through the combined measurement of dehydrogenase and urease activity, the specific reason for the functional attenuation of microorganisms can be distinguished; through the combined measurement of plant height and leaf area index, the stage of restricted vegetation growth can be identified; through the combined measurement of soil moisture content and pore water pressure, the type of water stress can be judged. Thus, the upgrade from single index alarm to multi-factor collaborative diagnosis is realized, and misjudgment or omission caused by data fragmentation is avoided.

[0070] The application further proposes a water conservancy project ecological revetment structure repair and maintenance method. In the dynamic monitoring and collaborative regulation steps, the preset threshold value is that the number of functional microbial populations is less than 10 6 CFU / g, and the preset coverage is that the vegetation coverage is less than 60%.

[0071] The preset threshold value refers to the minimum number of active bacteria required by functional microorganisms in the soil to maintain ecological restoration effect, which can be detected by plate colony counting method. When the detection value is lower than the threshold value, it indicates that the microbial activity is insufficient, and the microbial agent needs to be supplemented to restore the soil ecological function. The preset coverage refers to the critical index of vegetation restoration in the revetment area, which can be monitored by unmanned aerial vehicle aerial photography combined with image analysis technology. When the coverage is lower than the value, it indicates that the vegetation growth is blocked, and the soil nutrient supply needs to be improved in combination with microbial regulation.

[0072] Specifically, in the dynamic monitoring stage, the number of microorganisms is detected by regularly collecting soil samples. For example, when the number of nitrogen-fixing bacteria is detected to be less than one million colony forming units per gram of soil, the microbial agent supplement mechanism is automatically triggered, and the corresponding microbial species are accurately put into the degraded area. At the same time, when the vegetation coverage monitoring value is lower than 60%, the ratio and application amount of microbial agents are adjusted in combination with the soil nutrient detection results, such as nitrogen and phosphorus content data, to preferentially supplement phosphorus-solubilizing bacteria or nitrogen-fixing bacteria to promote vegetation regeneration.

[0073] Compared with the prior art, the traditional method lacks a clear microbial and vegetation collaborative regulation threshold, resulting in that the repair measures lag behind the actual degradation degree. For example, in the prior art, the timing of microbial agent supplement is determined by artificial experience, which is prone to problems of overuse or insufficient intervention. However, by setting a quantitative threshold, the present application can realize data-driven precise regulation and improve the efficiency of ecological restoration.

[0074] Through the above technical solution, the application can identify the risk of microbial activity decline and vegetation degradation in real time, start intervention measures before the ecological function is irreversibly damaged, and effectively maintain the stability of the revetment structure and the dynamic balance of the ecological system. For example, when the number of microorganisms approaches the threshold value, the system can give an early warning and automatically allocate microbial resources to avoid soil structure degradation caused by microbial inactivation.

[0075] As Figure 2 shown, the application further proposes a water conservancy project ecological revetment structure repair and maintenance system, which includes a structure repair module, an ecological monitoring module and a data processing module; the structure repair module contains a flexible structure unit for slope reinforcement, a rigid and flexible combined unit and a foundation protection unit, the flexible structure unit is provided with an ecological bag and a geogrid connecting assembly, the rigid and flexible combined unit is provided with a crack sealing member and a vegetation concrete spraying assembly, and the foundation protection unit includes a scour protection cushion layer and a filter layer; the ecological monitoring module is arranged in different water level partitions of the revetment and contains a vegetation growth monitoring assembly, a soil microorganism monitoring assembly and a hydrological environment monitoring assembly; the data processing module is in communication connection with the structure repair module and the ecological monitoring module, and is used for receiving monitoring data and generating repair and maintenance instructions.

[0076] The flexible structure unit refers to a revetment reinforcement structure constructed by flexible materials, which can be realized by combining ecological bags and geogrids, the ecological bags are filled with local soil and plant seeds, and the geogrids are connected with the slope through anchor pieces for dispersing load and promoting vegetation growth. The rigid and flexible combined unit refers to a composite unit with structural strength and ecological adaptability, which can be realized by combining prefabricated concrete members and sprayed vegetation concrete, the crack sealing member fills the structure gap with elastic sealant, and the vegetation concrete spraying assembly sprays the mixed seed matrix on the slope surface through a compressed air device. The foundation protection unit refers to an anti-scour structure arranged at the bottom of the revetment, which can be realized by combining a gravel cushion layer and a geotextile filter layer, the thickness of the anti-scour cushion layer can be 30-50 cm, and the filter layer is made of non-woven geotextile and graded sand and gravel laid alternately. The ecological monitoring module refers to a data acquisition system integrated with multiple types of sensors, which can be realized by a wireless transmission sensor network, the vegetation growth monitoring assembly contains a chlorophyll sensor and an infrared imager, the soil microorganism monitoring assembly contains a fluorescence quantitative PCR detector, and the hydrological environment monitoring assembly contains a multi-parameter water quality probe.

[0077] Specifically, the structure repair module provides differential reinforcement for different mechanical requirements of different areas of the revetment through the synergistic effect of flexible, rigid-flexible combined and foundation protection units. For example, the flexible structure unit forms a reinforced layer with drainage function on the surface layer of the slope, and the plant roots in the ecological bag and the geogrid jointly enhance the shear strength of the soil; the rigid-flexible combined unit implements closed treatment in the structure crack area, and the sprayed vegetation concrete restores the ecological function of the surface layer; the foundation protection unit forms an anti-erosion barrier at the bottom of the revetment, and the filter layer can prevent soil particles from being lost. The ecological monitoring module arranges a sensor network in different water level zones, such as water pressure resistant sensors in high water level zones and drought resistant monitoring equipment in low water level zones, to collect real-time vegetation growth state, microbial activity and hydrological parameters. The data processing module integrates multi-source data through a communication protocol, such as correlating and analyzing soil moisture content data and microbial activity data, and automatically generating a bacterial agent supplement instruction when a decrease in microbial activity and abnormal soil moisture content is detected.

[0078] Compared with the prior art, the traditional revetment system uses single structure reinforcement and independent ecological monitoring, such as only setting a concrete retaining wall with artificial vegetation patrol. The present scheme realizes physical integration of structure repair and ecological monitoring through modular design, such as simultaneously setting crack closure components and spray assembly in the rigid-flexible combined unit to simultaneously complete structure repair and ecological restoration. At the same time, the data processing module establishes a correlation model of structure stability and ecological parameters, such as fusion analysis of pore water pressure data and vegetation root development data, which can early warn the risk of slope instability, while the traditional method needs to process structure monitoring data and ecological data separately.

[0079] Through the above technical scheme, the present application can realize the coordinated operation of revetment structure stability maintenance and ecological function repair. For example, when the ecological monitoring module detects a decrease in the number of soil microorganisms in the low water level area, the data processing module can start the maintenance program of the foundation protection unit filter layer in the corresponding area, and adjust the proportion of the spray matrix of the rigid-flexible combined unit. This linkage mechanism solves the repeated repair effect problem caused by the disconnection between structure maintenance and ecological regulation in traditional technology, and reduces the frequency of manual intervention through automatic instruction generation.

[0080] The present application further proposes that the ecological monitoring module further comprises a distributed sensing assembly buried in the revetment soil and structure for collecting strain, moisture content and pore water pressure data; the data processing module has a structure stability evaluation model and an ecological function evaluation model built-in, which can output structure reinforcement suggestions and ecological regulation schemes according to the distributed sensing assembly and vegetation and microbial monitoring data.

[0081] The distributed sensing assembly refers to a sensor network buried in the revetment soil and structure, which can be implemented by using a fiber optic sensor or a micro pressure sensor array, covers different depths and key stress areas of the revetment through a distributed layout, and is used for capturing the strain distribution, water content gradient change and pore water pressure dynamic data in the soil in real time. The structure stability evaluation model refers to an analysis algorithm based on the relationship between the mechanical parameters and the soil response, which can be a hybrid model combining a finite element model and a machine learning algorithm, and can predict the potential instability risk of the revetment structure by inputting the strain and pore water pressure data. The ecological function evaluation model refers to a multi-dimensional analysis model integrating vegetation growth data and microbial activity data, which can be a dynamic threshold judgment and trend prediction algorithm, and can generate ecological regulation strategies in combination with soil nutrients and hydrological parameters.

[0082] Specifically, the distributed sensing assembly forms a three-dimensional monitoring network in the revetment soil, can identify the shear deformation trend in the slope through continuous collection of strain data, can judge the saturation state of the soil and the change of the seepage path in combination with the water content and pore water pressure data. The data processing module inputs the collected physical parameters into the structure stability evaluation model, locates the weak areas of the structure and generates reinforcement suggestions by simulating the stress distribution under different water level conditions; at the same time, the ecological function evaluation model analyzes the vegetation growth index and microbial activity data, and when the root development is blocked or the microbial activity is reduced, automatically matches the corresponding microbial agent supplement scheme and vegetation maintenance measures. The structure reinforcement suggestions and ecological regulation schemes are transmitted to the operation and maintenance terminal through a data interface, so as to realize the coordinated execution of the repair measures.

[0083] Compared with the prior art, the traditional revetment monitoring system only uses surface displacement sensors or single vegetation monitoring equipment, and the data collection dimension is limited to surface physical parameters or isolated ecological indicators, which cannot reflect the coupling relationship between the internal mechanical state of the soil and the ecological elements. The present scheme realizes the synchronous monitoring of the strain field and the seepage field in the structure through the distributed sensing network, and establishes a dynamic correlation evaluation system of the structure stability and the ecological function in combination with the double model linkage analysis mechanism, thereby solving the problem of the separation of structure repair and ecological maintenance decision in the traditional technology.

[0084] Through the above technical scheme, the present application realizes the collaborative monitoring of the internal mechanical state of the revetment structure and the surface ecological parameters, effectively identifies the early signs of ecological function degradation caused by soil creep, and avoids the vicious cycle of structure instability and ecological deterioration. At the same time, the intelligent decision mechanism based on model linkage can simultaneously optimize the structure reinforcement scheme and the microbial-vegetation regulation strategy, reduce the resource waste and time lag caused by step-by-step disposal, and significantly improve the long-term maintenance capability of the revetment system.

[0085] The above merely provides an example of the present application, and is not used to limit the protection scope of the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A method for repairing and maintaining an ecological revetment structure of a water conservancy project, characterized in that, The method comprises the following steps: S1. Screening and domestication of functional microorganisms: Collect samples from healthy soil and rhizosphere environment of the target revetment area, use selective medium to separate and purify microorganisms in the samples, and screen strains with nitrogen fixation, phosphorus solubilization, growth promotion and soil aggregate function enhancement; the strains are domesticated in a culture medium simulating the revetment environment, and the survival rate of the strains in the target revetment environment meets the preset requirements by adjusting the environmental factors in stages. S2. Construction of vegetation and microorganism synergistic remediation unit: According to the vegetation type corresponding to the water level partition of the revetment, the functional microorganisms domesticated in step S1 are specifically matched with the vegetation; using an ecological concrete prefabricated block containing a slow-release nutrient substrate as a carrier, the functional microbial agent, vegetation seeds, water retaining agent and organic fertilizer are mixed in proportion and filled into the pores of the prefabricated block to form a microbial, vegetation and carrier integrated remediation unit, wherein the water permeability of the carrier meets the preset water permeability requirement. S3. On-site precise layout of the synergistic remediation unit: According to the revetment structure stability evaluation results, the synergistic remediation unit is laid out in the slope collapse risk area, the vegetation degradation serious area and the strong hydraulic flushing area; according to the corresponding laying mode of different water level partitions, the depth of the prefabricated block embedded in the soil meets the preset depth requirement, and is fixed by a fixing member; a water permeable geotextile is laid within a predetermined range around the unit. S4. Dynamic monitoring and synergistic regulation: A microbial, vegetation and environmental multi-parameter monitoring system is constructed, and microbial activity sensors, vegetation growth sensors and environmental factor sensors are laid out in the remediation unit area; soil samples are collected regularly, and the change of the number of functional microbial populations is detected; when the number of the populations is lower than a preset threshold, the corresponding functional microbial agent is supplemented; according to the vegetation growth monitoring data, when the vegetation coverage is lower than a preset coverage, the types and amounts of microbial agents are adjusted in combination with the soil nutrient detection results.

2. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S1, the selective medium includes Asubel medium for isolating nitrogen-fixing bacteria and Monkingna medium for isolating phosphorus-solubilizing bacteria.

3. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S1, the environmental factors of the simulated revetment environment include temperature, pH value and salinity, and the gradient adjustment gradually approaches the actual environmental factor range of the target revetment at a preset interval.

4. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S2, the water level partition includes high water level area, middle water level area and low water level area; the high water level area vegetation is Salix integra and Salix matsudana, matched with water-tolerant nitrogen-fixing bacteria and cellulose-decomposing bacteria; the middle water level area vegetation is Phragmites australis and Acorus calamus, matched with phosphorus-solubilizing bacteria and rhizosphere microorganisms; the low water level area vegetation is Cynodon dactylon and Iris laevigata, matched with drought-resistant and soil-improving microbial flora.

5. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S2, the concentration of the functional microbial agent meets the preset concentration requirement, and the porosity of the prefabricated block meets the preset porosity requirement.

6. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S3, the corresponding arrangement mode includes: row-column arrangement in high water level area, plum blossom arrangement in medium water level area, and random arrangement in low water level area combined with pebble beach terrain; the preset depth requirement is that the embedded soil depth is not less than 20 cm, and the preset range is within 50 cm of the unit periphery.

7. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S4, the microbial activity sensor is used for monitoring dehydrogenase and urease activity; the vegetation growth sensor is used for monitoring plant height and leaf area index; and the environmental factor sensor is used for monitoring soil water content, pH value and pore water pressure.

8. The method for repairing and maintaining an ecological revetment structure of a hydraulic engineering according to claim 1, characterized in that: In step S4, the preset threshold is that the number of functional microbial population is less than 10 6 CFU / g, and the preset coverage is that the vegetation coverage is less than 60%.

9. A system for repair and maintenance of an ecological revetment structure of a water engineering work according to any one of claims 1 to 8, characterized in that The ecological monitoring module includes a structure repair module, an ecological monitoring module and a data processing module. The structure repair module includes a flexible structure unit for slope reinforcement, a rigid and flexible combination unit and a foundation protection unit, the flexible structure unit is provided with an ecological bag and a geogrid connection assembly, the rigid and flexible combination unit is provided with a crack sealing member and a vegetation concrete spraying assembly, and the foundation protection unit includes a scour protection cushion layer and a filter layer. The ecological monitoring module is arranged in different water level zones of the revetment and includes a vegetation growth monitoring assembly, a soil microbial monitoring assembly and a hydrological environment monitoring assembly. The data processing module is in communication connection with the structure repair module and the ecological monitoring module, is used for receiving monitoring data and generating repair and maintenance instructions.

10. The hydraulic engineering ecological revetment structure repair and maintenance system according to claim 9, characterized in that, The ecological monitoring module further includes a distributed sensing assembly, which is embedded in the revetment soil and structure, and is used for collecting strain, water content and pore water pressure data. The data processing module is provided with a structure stability evaluation model and an ecological function evaluation model, which can output structure reinforcement suggestions and ecological regulation schemes according to the distributed sensing assembly and the vegetation and microbial monitoring data.

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