Sensitive skin face moisturizing essence and preparation method thereof

Abstract

The invention discloses sensitive skin face moisturizing essence and a preparation method thereof, and belongs to the technical field of essence. Comprising a phase A and a phase B, wherein the phase A comprises lychee seed extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lens culinaris seed extract, trollius chinensis bunge extract, prinsepia utilis royle extract, deionized water, a thickening agent, a humectant, allantoin and butanediol; a phase B: squalane, silicone oil, an emulsifier, an emollient and a vetiver extract; phase C: antioxidant, preservative and (daily use) essence; the lychee seed extract, the giant salamander collagen freeze-dried powder, the aronia melanocarpa extract, the lens culinaris seed extract, the trollius brachypetalus extract, the prinsepia utilis royle extract and the vetiver extract are added in the preparation of the essence, and through the synergistic compatibility effect of all the components, the essence has a good moisturizing effect, achieves the effect of moisturizing the skin, and is free of toxic and side effects. The sensitive skin repairing problem is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of essences, and particularly relates to a sensitive skin facial moisturizing essence and a preparation method thereof. BACKGROUND

[0002] As a kind of high-efficiency skin care product, the formula design and preparation technology of essence have made remarkable progress in recent years. At present, the essence products existing in the market mainly include lotion type, water type and the like. These products play an important role in moisturizing, antioxidant, anti-aging and the like, and greatly meet the user groups with different skin qualities and needs.

[0003] Sensitive skin is a sub-health state. The natural barrier of the skin is damaged, and the self-defense ability is decreased, so that external irritants are easily penetrated, the sensory nerve signal transmission of the skin is enhanced, the tolerance threshold is reduced, the skin overreacts to weak external stimuli, the immune response of the skin is activated, the blood vessels are expanded, the inflammatory factors are released, and the skin is prone to subjective symptoms such as burning, stinging, itching and tightness, accompanied by or not accompanied by objective signs such as erythema, scales and telangiectasia. In recent years, in order to improve the efficacy of skin care products, various chemical additives such as emollients, moisturizers and skin conditioners are often added to the preparation of essence. The addition of these irritating additives to the essence will increase the burden of the skin of sensitive skin people, and cause the skin sensitive symptoms to be further deteriorated. Therefore, the research and development of skin care products for sensitive skin people has become a technical problem to be solved. SUMMARY

[0004] The purpose of the present application is to provide a sensitive skin facial moisturizing essence and a preparation method thereof, which is used for improving the skin conditioning effect of sensitive skin people.

[0005] The purpose of the present application can be achieved by the following technical solutions.

[0006] A sensitive skin facial moisturizing essence, comprising:

[0007] A phase: litchi kernel extract, giant salamander collagen protein freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Prinsepia uniflora extract, deionized water, thickening agent, moisturizer, allantoin and butanediol;

[0008] B phase: squalane, silicone oil, emulsifier, emollient and miscanthus sinensis extract;

[0009] C phase: antioxidant, preservative and (daily) fragrance.

[0010] As a preferred technical scheme of the present application, in the A phase, the mass ratio of the litchi seed extract, the Hachi-fushi collagen freeze-dried powder, the Aronia melanocarpa extract, the Lens culinaris seed extract, the Gypsophila oldhamiana extract, the Prinsepia uniflora fruit extract, the deionized water, the thickening agent, the humectant, allantoin, and butanediol is 1.2-2.4:1.8-3.6:1.4-1.8:1.2-1.4:1.8-2.2:1.8-2.6:74.7-81.9:0.3-0.4:0.4-0.6:0.2-0.3:8-10.

[0011] As a preferred technical scheme of the present application, in the B phase, the mass ratio of the squalane, the silicone oil, the emulsifier, the emollient, and the Helictotrichon arundinaceum extract is 0.7-0.9:0.4-0.5:0.02-0.04:0.04-0.06:1.2-1.4.

[0012] As a preferred technical scheme of the present application, in the C phase, the mass ratio of the antioxidant, the preservative, and the (daily use) perfume is 3-7:1-3:2-4.

[0013] As a preferred technical scheme of the present application, the preparation method of the litchi seed extract comprises the following steps:

[0014] Fresh litchi is peeled and the pulp is removed to obtain litchi seeds, which are cleaned, crushed, and passed through an 80-mesh screen to obtain a litchi seed powder. The litchi seed powder is added to deionized water, and the pH is adjusted to 4.5 with an acid solution. A surfactant and cellulase are added, and stirring is performed at 45°C for 40-80 min. Then, water extraction is performed at 80°C while stirring for 2 h. The solution is concentrated to 10 mL under vacuum using a rotary evaporator. Then, 80 mL of an ethanol solution with a volume fraction of 75% is added, and the mixture is sealed and allowed to stand for 24 h. Filtration is performed, and the filter cake is washed three times with an ethanol solution with a volume fraction of 75%. The ethanol is removed by water bath evaporation at 65°C, and the litchi seed extract is obtained.

[0015] The surfactant is selected from any one of Tween-80, sodium dodecyl sulfate, and glycerol monostearate;

[0016] The litchi seed powder, deionized water, surfactant, and cellulase are mixed in a ratio of 15 g:200 mL:0.4 g:300000 U.

[0017] As a preferred technical scheme of the present application, the preparation method of the Hachi-fushi collagen freeze-dried powder comprises the following steps:

[0018] The healthy artificially cultivated giant salamanders are selected, the skin tissues are obtained, the redundant muscle tissues and pigments are removed, then the skin tissues are washed with deionized water, air-dried, weighed, then cut into small pieces with a size of 2*2 cm; the air-dried small pieces are added into a clean beaker with a capacity of 2 L, anhydrous ether is added, so that the material-liquid ratio is 1:10 (g / mL), the beaker opening is sealed with a preservative film, and magnetic stirring is carried out for 24 h; after the first defatting, filtration is carried out, and the solid phase is reserved; after the residual solvent is volatilized dry, 10% n-butanol by volume fraction is added, so that the material-liquid ratio is 1:10 (g / mL), the beaker opening is sealed with a preservative film, and magnetic stirring is carried out for 24 h; after the second defatting, filtration is carried out, and the solid phase is reserved; the solid phase is washed with deionized water until neutral, air-dried, then 0.1 mol / L sodium hydroxide solution is added, so that the material-liquid ratio is 1:20 (g / mL), then the removal of impurities is carried out, the beaker opening is sealed with a preservative film, and magnetic stirring is carried out for 24 h; the solvent is replaced every 8 h; separation is carried out, the solid phase is washed with deionized water until neutral, drained, then air-dried; after the surface is dry, acid dissolution is carried out, 0.5 mol / L acetic acid solution is added, so that the material-liquid ratio is 1:60 (g / mL), magnetic stirring is carried out for 24 h, filtration is carried out, the filtrate is taken, sodium chloride is added to the filtrate until the concentration of sodium chloride in the filtrate is 2.5 mol / L, salting-out is carried out for 12 h, filtration is carried out, and the precipitate is reserved; the obtained precipitate is dissolved with 0.1 mol / L acetic acid, then the solution is filled into a dialysis bag, placed in a large beaker, and dialyzed; first, 0.1 mol / L acetic acid is used for dialysis, and the solvent is replaced every 8 h until the chloride ions are removed (tested with AgNO3 solution); then, ultrapure water is used for dialysis, and the solvent is replaced every 8 h until the outside liquid is neutral (tested with pH test paper); the dialyzed collagen is poured into a flat dish, sealed with a negative preservative film, and perforated with a plurality of small holes, and placed in a refrigerator at-18℃ for pre-freezing for 24 h; then, the pre-frozen collagen is placed in a vacuum freeze dryer and freeze-dried at-45℃ for 20 h; the freeze-dried crude extract is dissolved in 0.2 mol / L acetic acid solution, and a collagen protein solution with a concentration of 0.5% is prepared; neutral protease is added to the collagen protein solution for enzymolysis; the enzymolysis conditions are as follows: the enzymolysis time is 5 h, the temperature is 45℃, the pH is 6.8, the enzyme dosage is 5000 U / g, and the material-liquid ratio is 1:10 (g / mL); after the enzymolysis is completed, the reaction liquid is placed in a 90℃ water bath for 10 min to inactivate the enzyme activity; the enzyme-inactivated enzymolysis product is centrifuged and separated, the supernatant is collected, filtered through a 0.45 mu m filter membrane, dialyzed and purified for 10 h, then transferred to a freeze dryer, and freeze-dried under the condition of-45℃, so that the giant salamander collagen freeze-dried powder is obtained.

[0019] As a preferred technical scheme of the present application, the preparation method of the black chokeberry extract comprises the following steps:

[0020] Fresh fruits of A. melanocarpa were washed with deionized water for 3 times, and then cut into small pieces of 1-2 cm in size. The small pieces were put into a freeze-drying machine (-50℃, vacuum degree 10 Pa) and freeze-dried for 24 h until completely dried (water content ≤5%). The dried fruits were ground and passed through a 40-mesh sieve. The powder was collected and put into a sealed bag, which was stored in a desiccator for later use.

[0021] Determination of total phenolic content: Soxhlet extraction: Take qualitative filter paper (diameter 12 cm), weigh 10 g of dried Aronia melanocarpa powder in the center, fold into a cylindrical filter paper package (height lower than 2 / 3 of the height of the Soxhlet extractor extraction tube), tie the opening end with cotton thread, mark the sample information, add 100 mL of diethyl ether (analytical pure) to a 250 mL round-bottom flask, put the filter paper package into the extraction tube, make sure the filter paper package is completely immersed in diethyl ether, connect the extraction tube, spherical condenser in turn, the condenser is connected with cooling water, place the device in a constant temperature water bath, set the temperature to 54°C, extract for 5 h, after extraction, take out the filter paper package, dry naturally until there is no diethyl ether smell, put the dried filter paper package into a 500 mL three-necked flask, add 400 mL of deionized water preheated to 77°C according to the liquid-solid ratio of 1:40 (g / mL), extract for 60 min, after extraction, pour the mixture into a funnel while hot, open the vacuum pump to filter, collect the filtrate in a 500 mL beaker, wash the inner wall of the three-necked flask and the filter paper package with 50 mL of hot water at 77°C, the washing liquid is added to the filtrate, the filter residue (together with the filter paper package) is put back into the three-necked flask, add 400 mL of hot water at 77°C according to the same liquid-solid ratio, repeat the above extraction and filtration steps, collect the second filtrate, combine the two filtrates, transfer into a 1 L beaker, stir uniformly with a glass rod, transfer the combined filtrate to a 500 mL evaporation flask of a rotary evaporator, set the parameters: water bath temperature 55°C, rotation speed 60 r / min, vacuum degree 0.09MPa, concentrated to a volume of 200 mL, and after the end of the concentration, the concentrated solution was transferred to a 250 mL beaker, the evaporation flask was rinsed with a small amount of deionized water, and the rinse was added to the beaker (total volume 210 mL), a 93% ethanol solution with a volume of 630 mL (3 times the volume of the concentrated solution) was prepared and placed in a 4°C refrigerator for pre-cooling for 30 min, under magnetic stirring, the pre-cooled 93% ethanol solution was slowly added to the concentrated solution, after the addition was completed, stirring was continued for 10 min to ensure uniform mixing of the solution, the mixed solution was transferred to a 500 mL conical flask with a stopper, the mouth of the flask was sealed, and the flask was placed in a 4°C refrigerator overnight to allow the polysaccharides to fully precipitate, the mixed solution after standing was divided into 50 mL centrifuge tubes, centrifuged, and the precipitate at the bottom of the tube was retained, 20 mL of a 93% ethanol solution was added to each centrifuge tube, the precipitate was gently stirred with a glass rod to disperse it, and ultrasonic treatment was performed for 2 min (power 300 W), the centrifugation operation was repeated 2-3 times until the supernatant was colorless, 20 mL of ether (analytical pure) was used instead of ethanol, and the above dispersion-ultrasonic- centrifugation steps were repeated 2-3 times to remove residual fat-soluble impurities, after the last centrifugation, the precipitate was transferred to a petri dish and placed in a fume hood to dry for 30 min until there was no ether smell, the dried precipitate was weighed, 80°C deionized water was added according to the solid-liquid ratio of 1:20 (g / mL), and magnetic stirring was performed for 30 min to obtain a polysaccharide solution, which was transferred to a 250 mL separatory funnel, and a mixture of chloroform:n-butanol=4:1 (volume ratio) was added, the volume of the mixture was 1 / 5 of the volume of the polysaccharide solution, the stopper of the separatory funnel was tightly covered, and the separatory funnel was inverted and shaken for 5 min, then placed in a reciprocating shaker and set to rotate at 180 r / min for 30 min, the separatory funnel was taken out and allowed to stand for 30 min until the layers were clear (the upper layer was the aqueous phase containing polysaccharides, the middle layer was the protein layer, and the lower layer was the organic phase), the stopcock was slowly opened, and the upper aqueous phase was retained, the above operation (addition of mixture-vibration- standing-separation) was repeated 5 times until the middle protein layer disappeared, the deproteinized aqueous phase was transferred to a pretreated dialysis bag (MD44-10000 dialysis bag, molecular weight cut-off 10000 Da, cut into 20 cm long sections, boiled in 0.5 mol / L EDTA solution (pH 8.0) for 10 min, then boiled in deionized water for 10 min, and soaked in deionized water after cooling for standby), the two ends were tied with cotton thread (1 / 3 space was left to prevent swelling and rupture), placed in a 5 L beaker, 4 L of deionized water was added (the liquid level reached the dialysis bag), and the mixture was slowly stirred with a magnetic stirrer (stirring speed 50 r / min), and placed in a 4°C refrigerator for dialysis for 12 h, during which the deionized water was replaced every 4 h, after dialysis, the polysaccharide solution in the dialysis bag was transferred to a freeze-drying bottle, placed in a -80°C ultra-low temperature refrigerator for pre-freezing for 4 h until completely frozen, transferred to a freeze-drying machine, and the parameters were set as follows: vacuum degree 10 Pa, cold trap temperature -55°C, and freeze-drying for 24 h until the sample was in the form of loose powder, the freeze-dried powder was taken out and gently ground with a mortar, and passed through an 80 mesh sieve to obtain the blackcurrant extract.

[0022] As a preferred technical scheme of the present application, the preparation method of the lentil seed extract comprises the following steps:

[0023] The washed and dried lentil seeds are crushed, passed through an 80-mesh screen, and 10 g of the undersize powder is collected and placed in a 250-mL round-bottom flask. A 70% ethanol solution is added at a solid-to-liquid ratio of 1:15 (g / mL), and the mixture is extracted in a 78℃ water bath for 3 times, with each single extraction lasting 2 h. After extraction, the mixture is cooled to room temperature, pre-filtered through double-layered gauze, and then filtered through a 0.45-μm microporous filter. The filtrate is collected and concentrated to 1 / 5 of the original volume in a rotary evaporator. The concentrated solution is frozen at -45℃ for 24 h, and then freeze-dried to obtain the lentil seed extract.

[0024] As a preferred technical scheme of the present application, the preparation method of the short-petaled Trollius extract comprises the following steps:

[0025] The short-petaled Trollius flowers (with intact petals and calyces) are washed, dried, crushed, and sieved through an 80-mesh screen. 10 g of the undersize powder is collected and placed in a 2-L conical flask with a stopper. A 60% ethanol solution is added at a solid-to-liquid ratio of 1:30 (g / mL), and the mixture is stirred uniformly. The mixture is then placed in a constant-temperature water bath shaker (set at 60℃ and 180 r / min) for timed extraction for 140 min. After extraction, the mixture is filtered at normal pressure using double-layered qualitative filter paper, and the filtrate is collected in a 500-mL beaker. The inner wall of the conical flask and the filter residue are rinsed with 20 mL of a 60% ethanol solution, and the rinse is added to the filtrate. A rotary evaporator is started to concentrate the mixture under reduced pressure (water bath temperature 45℃, vacuum degree 0.09 MPa, and rotation speed 50 r / min). The mixture is concentrated to 1 / 5 of the original volume, and the concentrated solution is transferred to a vacuum drying oven (set at 50℃ and 0.09 MPa) for drying for 6 h to obtain the short-petaled Trollius crude extract.

[0026] 50 g of macroporous adsorption resin AB-8 is placed in a 500-mL beaker, and 200 mL of a 95% ethanol solution is added. The mixture is soaked for 24 h. Then, the mixture is washed by reduced-pressure filtration with a 95% ethanol solution for 3 times until the eluate is clear after dilution with deionized water. The mixture is then soaked with a 5% hydrochloric acid solution for 2 h, and then filtered and washed with deionized water until the pH is neutral. Finally, the mixture is soaked with a 5% sodium hydroxide solution for 2 h, and then filtered and washed with deionized water until the pH is neutral. The pretreated macroporous adsorption resin is obtained and ready for use.

[0027] The pretreated AB-8 resin was loaded into a glass chromatography column (specification: diameter 2.5 cm, height 30 cm) by wet loading method, and the bed height was controlled at 20 cm; the resin column was washed with 5 mL / min flow rate of acetic acid-sodium acetate buffer solution (preparation: 0.1 mol / L acetic acid solution and 0.1 mol / L sodium acetate solution were mixed at a volume ratio of 15:1, and the pH was calibrated by a pH meter) with pH 4.0 until the effluent pH was stable at 4.0, and the equilibrium was completed;

[0028] 1 g of short-petal Trollius chinensis Bunge crude extract was weighed, 100 mL of acetic acid-sodium acetate buffer solution with pH 4.0 was added, and magnetic stirring was performed for 30 min to obtain a sample solution; the sample solution was pumped into the chromatography column at a constant flow rate of 10 mL / min; during the sample loading process, 5 mL of effluent was collected every 10 min, and when the target spot (consistent with the control) appeared in the effluent detected by thin layer chromatography (TLC), the sample loading was stopped; after the sample loading was completed, the chromatography column was washed with deionized water at a flow rate of 10 mL / min until the effluent was colorless and transparent, and the water-soluble impurities were removed; the part of the eluent was discarded, and 50% ethanol solution was used to elute at a flow rate of 8 mL / min; the eluent was collected in sections (20 mL each time), and the effective eluent was combined; the combined effective eluent was transferred to a 500 mL rotary evaporator flask, and the rotary evaporator was started with the following parameters: water bath temperature 45℃, vacuum degree 0.08 MPa, and rotation speed 55 r / min; the eluent was concentrated to a volume of 30 mL; after the concentration was completed, a small amount of deionized water (5 mL) was used to rinse the inner wall of the evaporator flask; the concentrated eluent was combined into a 50 mL freeze-drying flask, which was placed in a-80℃ ultra-low temperature refrigerator for 5 h until the concentrated eluent was completely frozen; the pre-frozen freeze-drying flask was transferred to a freeze-drying machine, and the freeze-drying was performed at a cold trap temperature of-55℃ and a vacuum degree of 10 Pa for 24 h; the freeze-dried powder was taken out, ground, and passed through an 80-mesh sieve to obtain the short-petal Trollius chinensis Bunge extract.

[0029] As a preferred technical scheme of the present application, the preparation method of the Prinsepia uniflora extract comprises the following steps:

[0030] The residue after extracting oil from Prinsepia uniflora seed is selected, the shells, impurities and un-extracted seed kernels are removed, the loose seed meal debris is retained, dried in a 50℃ air-drying oven for 5h (water content is reduced to ≤8%), crushed, passed through an 80 mesh sieve, 100g of the sieved powder is collected and poured into a 2L microwave extraction beaker; deionized water is added at a solid-liquid ratio of 1:10 (g / mL), microwave treatment is performed (microwave power is set to 250W, magnetic stirring speed is 200r / min, microwave time is 60min in total), the mixed solution after microwave treatment is transferred to a 2L round-bottom flask, a reflux condenser is connected, the top of the condenser is connected to a vacuum line (connected to a vacuum pump) and a thermometer interface, the flask is placed in a constant temperature water bath, the vacuum degree is adjusted to 0.05MPa, the water bath temperature is 40℃, and extraction is performed for 90min; after extraction, the hot solution is filtered through a 200 mesh filter cloth, the filtrate is collected in a 1L beaker, the filter residue is washed with 50mL of 40℃ distilled water for 2 times, the washing liquid is combined with the filtrate, a rotary evaporator is started (water bath temperature is 45℃, vacuum degree is 0.08MPa, and rotation speed is 60r / min), and the concentration is stopped when the volume is reduced to 1 / 4 of the original volume, and a concentrated solution is obtained;

[0031] The concentrated solution is placed in a 5L beaker, a constant flow pump is used to add a 95% ethanol solution to the concentrated solution at a speed of 140mL / min under magnetic stirring, after the addition is completed, stirring is continued for 10min, the solution is placed in a 4℃ refrigerator for 2h, and then the filter cake is collected by vacuum filtration (vacuum degree is 0.06MPa) through a 200 mesh filter cloth, transferred to a freeze-drying bottle, pre-frozen in a-80℃ ultra-low temperature refrigerator for 4h, and then freeze-dried in a freeze-drying machine at a cold trap temperature of-55℃ and a vacuum degree of 10Pa for 24h, ground and passed through an 80 mesh sieve to obtain the Prinsepia uniflora extract;

[0032] As a preferred technical solution of the present application, the preparation method of the Phlox subulata extract comprises the following steps:

[0033] Fresh Phlox subulata roots are selected, washed, dried, crushed, passed through a 20 mesh sieve, and the sieved powder is collected;

[0034] A 1000 mL round bottom flask is added with 500 mL of deionized water, a distillation support with a sieve plate (sieve plate aperture 5 mm) is connected, 300 g of an orchid root powder is evenly spread on the sieve plate, a layer of defatted cotton is covered on top, a condenser tube is connected in turn, a tail pipe is connected, the tail pipe is inserted into a separatory funnel, the whole device is sealed, after the water in the flask boils to generate stable water vapor, the heating power is adjusted to stabilize the vapor generation rate (the drop speed of the distillate at the outlet of the condenser tube is 1-2 drops per second), the distillation time is 5 h, the distillation is stopped, the distillate is transferred to the separatory funnel, and after standing for 30 min, the lower layer of the crude essential oil is slowly released, the crude essential oil is taken into a conical flask, anhydrous sodium sulfate (the amount is 10% of the mass of the essential oil) is added, it is sealed and placed on a shaking bed for 30 min, and then the dehydrated essential oil is filtered with a qualitative filter paper to remove the anhydrous sodium sulfate particles, and the preliminary purified essential oil is obtained, the preliminary purified essential oil is frozen in a refrigerator at-5℃ for 24 h, the wax is crystallized and precipitated, then a pre-cooled to 0℃ Burdick & Jackson funnel is used for suction filtration, and the filtrate is collected, and the orchid extract is obtained.

[0035] As a preferred technical solution of the present application, the thickening agent is selected from at least one of xanthan gum, carbomer and hydroxyethyl cellulose.

[0036] As a preferred technical solution of the present application, the humectant is selected from at least one of sodium hyaluronate and 1,2-hexanediol.

[0037] As a preferred technical solution of the present application, the emulsifier is selected from at least one of PEG-10 dimethicone and PEG-3 dimethicone.

[0038] As a preferred technical solution of the present application, the emollient is selected from at least one of ethylhexylglycerin, cetearyl alcohol stearate and cetyl palmitate.

[0039] The preparation method of the sensitive skin facial moisturizing essence liquid comprises the following steps:

[0040] S1, the lychee kernel extract, giant salamander collagen protein freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Chinese olive fruit extract, deionized water, thickening agent, humectant, allantoin and butanediol are mixed, heated to 40-50℃, and stirred for 8-10 min to obtain phase A;

[0041] S2, the squalane, silicone oil, emulsifier, emollient and orchid extract are mixed, heated to 70-80℃, and stirred for 5-10 min to obtain phase B;

[0042] S3. Add phase B to phase A, and homogenize and stir at 8000-12000 r / min for 5-10 min at 45°C. Add phase C and homogenize and stir at 8000 r / min for 3-5 min to obtain the sensitive skin facial moisturizing essence.

[0043] As a preferred embodiment of the present invention, the mass ratio of phase A, phase B, and phase C is 73-79:21-27:0.3-0.5.

[0044] As a preferred embodiment of the present invention, the antioxidant is selected from at least one of tocopherol acetate and 3-o-ethyl ascorbic acid.

[0045] As a preferred embodiment of the present invention, the preservative is potassium sorbate.

[0046] The beneficial effects of this invention are:

[0047] This invention incorporates lychee seed extract, giant salamander collagen freeze-dried powder, black chokeberry extract, lentil seed extract, short-petaled golden lotus extract, sea buckthorn fruit extract, and vetiver extract into the preparation of the essence. Through the synergistic effect of these components, it achieves excellent moisturizing effects, resulting in hydrated skin. It also reduces the content of chemical additives and introduces a variety of natural extracts that are more compatible with human skin to improve and repair sensitive skin problems. Detailed Implementation

[0048] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0049] Preparation Example 1

[0050] The preparation method of litchi seed extract includes the following steps:

[0051] Peel and remove the pulp from fresh lychees to obtain lychee seeds. After cleaning, crush the seeds and pass them through an 80-mesh sieve to obtain seed powder. Add the powder to deionized water and adjust the pH to 4.5 with an acid solution. Add surfactant and cellulase, stir at 45℃ for 60 min, and then reflux at 80℃ for 2 h with stirring. Concentrate the powder to 10 mL using a rotary evaporator. Add 80 mL of 75% ethanol solution, seal and let stand for 24 h. Filter the powder, wash the filter cake three times with 75% ethanol solution, and evaporate the ethanol in a 65℃ water bath to obtain the lychee seed extract.

[0052] The surfactant is sodium dodecyl sulfate;

[0053] The dosing ratio of the core powder, deionized water, surfactant and cellulase is 15 g: 200 mL: 0.4 g: 300000 U.

[0054] Preparation Example 2

[0055] The preparation method of the Andrias davidianus collagen protein freeze-dried powder comprises the following steps:

[0056] The healthy artificial breeding giant salamanders are selected, the skin tissue is obtained, the redundant muscle tissue and pigment are removed, then the skin tissue is washed with deionized water, air-dried, weighed, cut into small pieces with a size of 2*2 cm, the air-dried small pieces are added into a clean beaker with a capacity of 2 L, anhydrous ether is added, so that the material-liquid ratio is 1:10 (g / mL), the beaker opening is sealed with a fresh-keeping film, magnetic stirring is carried out for 24 h, after the first defatting, filtration is carried out, the solid phase is reserved, after the residual solvent is volatilized, 10% n-butanol by volume fraction is added, so that the material-liquid ratio is 1:10 (g / mL), the beaker opening is sealed with a fresh-keeping film, magnetic stirring is carried out for 24 h, after the second defatting, filtration is carried out, the solid phase is reserved, the solid phase is washed with deionized water until neutral, air-dried, 0.1 mol / L sodium hydroxide solution is added, so that the material-liquid ratio is 1:20 (g / mL), then the removal of impurities is carried out, the beaker opening is sealed with a fresh-keeping film, magnetic stirring is carried out for 24 h, the solvent is replaced every 8 h, separation is carried out, the solid phase is washed with deionized water until neutral, drained, air-dried, after the surface is dry, acid dissolution is carried out, 0.5 mol / L acetic acid solution is added, so that the material-liquid ratio is 1:60 (g / mL), magnetic stirring is carried out for 24 h, filtration is carried out, the filtrate is taken, sodium chloride is added to the filtrate until the concentration of sodium chloride in the filtrate is 2.5 mol / L, salting-out is carried out for 12 h, filtration is carried out, the precipitate is reserved, the obtained precipitate is dissolved with 0.1 mol / L acetic acid, then the solution is filled into a dialysis bag, placed in a large beaker for dialysis, first, 0.1 mol / L acetic acid is used for dialysis, the solvent is replaced every 8 h, until the chloride ions are removed (tested with AgNO3 solution), then, ultrapure water is used for dialysis, the solvent is replaced every 8 h, until the outside liquid of dialysis is neutral (tested with pH test paper), the dialyzed collagen is poured into a flat dish, sealed with a fresh-keeping film, and a plurality of small holes are punched, placed in a refrigerator at-18℃ for pre-freezing for 24 h, then the pre-frozen collagen is placed in a vacuum freeze dryer and freeze-dried at-45℃ for 20 h, the freeze-dried crude extract is dissolved in 0.2 mol / L acetic acid solution, prepared into a collagen protein solution with a concentration of 0.5%, neutral protease is added to the collagen protein solution for enzymolysis, the enzymolysis conditions are as follows: the enzymolysis time is 5 h, the temperature is 45℃, the pH is 6.8, the enzyme amount is 5000 U / g, and the material-liquid ratio is 1:10 (g / mL); after the enzymolysis is completed, the reaction liquid is placed in a 90℃ water bath for 10 min to inactivate the enzyme activity, the enzyme-inactivated enzymolysis product is centrifuged and separated, the supernatant is collected, filtered through a 0.45 μm filter membrane, after the filtration, the filtered solution is dialyzed and purified for 10 h, then transferred to a freeze dryer, freeze-dried under the condition of-45℃, and the giant salamander collagen freeze-dried powder is obtained.

[0057] Preparation Example 3

[0058] The preparation method of the black chokeberry extract comprises the following steps:

[0059] Fresh fruits of A. melanocarpa were washed with deionized water for 3 times, and then cut into small pieces of 1-2 cm in size. The small pieces were put into a freeze-drying machine (-50℃, vacuum degree 10 Pa) and freeze-dried for 24 h until completely dried (water content ≤5%). The dried fruits were ground and passed through a 40-mesh sieve. The powder was collected and put into a sealed bag, which was stored in a desiccator for later use.

[0060] Determination of total phenolic content: Soxhlet extraction: Take qualitative filter paper (diameter 12 cm), weigh 10 g of dried Aronia melanocarpa powder in the center, fold into a cylindrical filter paper package (height lower than 2 / 3 of the height of the Soxhlet extractor extraction tube), tie the opening end with cotton thread, mark the sample information, add 100 mL of diethyl ether (analytical pure) to a 250 mL round-bottom flask, put the filter paper package into the extraction tube, make sure the filter paper package is completely immersed in diethyl ether, connect the extraction tube, spherical condenser in turn, the condenser is connected with cooling water, place the device in a constant temperature water bath, set the temperature to 54°C, extract for 5 h, after extraction, take out the filter paper package, dry naturally until there is no diethyl ether smell, put the dried filter paper package into a 500 mL three-necked flask, add 400 mL of deionized water preheated to 77°C according to the liquid-solid ratio of 1:40 (g / mL), extract for 60 min, after extraction, pour the mixture into a funnel while hot, open the vacuum pump to filter, collect the filtrate in a 500 mL beaker, wash the inner wall of the three-necked flask and the filter paper package with 50 mL of hot water at 77°C, the washing liquid is added to the filtrate, the filter residue (together with the filter paper package) is put back into the three-necked flask, add 400 mL of hot water at 77°C according to the same liquid-solid ratio, repeat the above extraction and filtration steps, collect the second filtrate, combine the two filtrates, transfer into a 1 L beaker, stir uniformly with a glass rod, transfer the combined filtrate to a 500 mL evaporation flask of a rotary evaporator, set the parameters: water bath temperature 55°C, rotation speed 60 r / min, vacuum degree 0.09MPa, concentrated to a volume of 200 mL, and after the end of the concentration, the concentrated solution was transferred to a 250 mL beaker, the evaporation flask was rinsed with a small amount of deionized water, and the rinse was added to the beaker (total volume 210 mL). A 93% ethanol solution was prepared by measuring 630 mL (3 times the volume of the concentrated solution) and placing it in a 4°C refrigerator for 30 minutes. Under magnetic stirring, the pre-cooled 93% ethanol solution was slowly added to the concentrated solution. After the addition was complete, stirring was continued for 10 minutes to ensure uniform mixing of the solution. The mixture was then transferred to a 500 mL conical flask with a stopper, the opening was sealed, and the flask was placed in a 4°C refrigerator overnight to allow the polysaccharides to fully precipitate. The mixture after standing was divided into 50 mL centrifuge tubes, centrifuged, and the precipitate at the bottom of the tubes was retained. To each centrifuge tube, 20 mL of a 93% ethanol solution was added, and the precipitate was gently stirred with a glass rod to disperse it. Ultrasonic treatment was performed for 2 minutes (power 300 W), and the centrifugation operation was repeated 3 times until the supernatant was colorless. 20 mL of ether (analytical pure) was used instead of ethanol, and the above dispersion-ultrasonic- centrifugation steps were repeated 2-3 times to remove residual fat-soluble impurities. After the last centrifugation, the precipitate was transferred to a petri dish and placed in a fume hood to dry for 30 minutes until there was no ether smell. The dried precipitate was weighed and added to 80°C deionized water at a ratio of 1:20 (g / mL) under magnetic stirring for 30 minutes to obtain a polysaccharide solution. The solution was transferred to a 250 mL separatory funnel and mixed with a chloroform:n-butanol=4:1 (volume ratio) mixture, with the volume of the mixture being 1 / 5 of the volume of the polysaccharide solution. The stopper of the separatory funnel was tightly closed, inverted and shaken for 5 minutes, then placed in a reciprocating shaker set at 180 r / min for 30 minutes. The separatory funnel was removed and allowed to stand for 30 minutes until the layers were clear (the upper layer was the water phase containing polysaccharides, the middle layer was the protein layer, and the lower layer was the organic phase). The stopcock was slowly opened, and the upper water phase was retained. The above operations (addition of mixture-shaking- standing-separation) were repeated 5 times until the middle protein layer disappeared. The deproteinized water phase was transferred to a pretreated dialysis bag (MD44-10000 dialysis bag, molecular weight cutoff 10000 Da, cut into 20 cm long sections, boiled in 0.5 mol / L EDTA solution (pH 8.0) for 10 minutes, then boiled in deionized water for 10 minutes, and cooled in deionized water for use). The two ends were tied with cotton thread (leaving 1 / 3 space to prevent swelling and breaking), placed in a 5 L beaker, and added with 4 L deionized water (the liquid level reached the dialysis bag). Slow stirring was performed with a magnetic stirrer (stirring speed 50 r / min), and the dialysis was carried out in a 4°C refrigerator for 12 hours. The deionized water was replaced every 4 hours during the dialysis. After the dialysis was completed, the polysaccharide solution in the dialysis bag was transferred to a freeze-drying bottle, placed in a -80°C ultra-low temperature refrigerator for 4 hours until it was completely frozen, and then transferred to a freeze-drying machine. The parameters were set as follows: vacuum degree 10 Pa, cold trap temperature -55°C, and freeze-drying time 24 hours until the sample was in the form of loose powder. The freeze-dried powder was gently ground with a mortar and sieved through an 80 mesh sieve to obtain the blackcurrant extract.

[0061] Preparation Example 4

[0062] The preparation method of the lentil seed extract comprises the following steps:

[0063] The washed and dried lentil seeds were ground and passed through an 80-mesh screen. 10 g of the sieved powder was placed in a 250-mL round-bottom flask, and 70% ethanol solution was added at a solid-liquid ratio of 1:15 (g / mL). The mixture was extracted in a water bath at 78°C for 3 times, with each single extraction lasting for 2 h. After extraction, the mixture was cooled to room temperature, pre-filtered with double-layer gauze, and then filtered with a 0.45-μm microporous filter membrane. The filtrate was collected and concentrated to 1 / 5 of the original volume in a rotary evaporator. The concentrated solution was frozen at -45°C and freeze-dried for 24 h to obtain the lentil seed extract.

[0064] Preparation Example 5

[0065] The preparation method of the short-petaled Trollius extract comprises the following steps:

[0066] The short-petaled Trollius flowers (with intact petals and calyxes) were washed, dried, ground, and sieved with an 80-mesh screen. 10 g of the sieved powder was placed in a 2-L conical flask with a stopper, and 60% ethanol solution was added at a solid-liquid ratio of 1:30 (g / mL) and stirred uniformly. The mixture was extracted in a constant-temperature water bath shaker (set at 60°C and 180 r / min) for 140 min. After extraction, the mixture was filtered at normal pressure with double-layer qualitative filter paper, and the filtrate was collected in a 500-mL beaker. The inner wall of the conical flask and the filter residue were washed with 20 mL of 60% ethanol solution, and the washing solution was added to the filtrate. The rotary evaporator was started to concentrate the mixture under reduced pressure (water bath temperature 45°C, vacuum degree 0.09 MPa, and rotation speed 50 r / min). The mixture was concentrated to 1 / 5 of the original volume, and the concentrated solution was transferred to a vacuum drying oven (set at 50°C and 0.09 MPa) for drying for 6 h to obtain the short-petaled Trollius crude extract.

[0067] 50 g of macroporous adsorption resin AB-8 was placed in a 500-mL beaker, and 200 mL of 95% ethanol solution was added for soaking for 24 h. Then, the macroporous adsorption resin was washed with 95% ethanol solution by reduced-pressure filtration for 3 times until the eluate was clear after dilution with deionized water. The macroporous adsorption resin was then soaked with 5% hydrochloric acid solution for 2 h, filtered, and washed with deionized water until the pH was neutral. Finally, the macroporous adsorption resin was soaked with 5% sodium hydroxide solution for 2 h, filtered, and washed with deionized water until the pH was neutral. The pretreated macroporous adsorption resin was obtained and reserved for use.

[0068] The pretreated AB-8 resin was loaded into a glass chromatography column (specification: diameter 2.5 cm, height 30 cm) by wet loading, and the bed height was controlled at 20 cm; the resin column was washed with 5 mL / min flow rate of acetic acid-sodium acetate buffer solution (preparation: 0.1 mol / L acetic acid solution and 0.1 mol / L sodium acetate solution were mixed at a volume ratio of 15:1, and the pH was calibrated with a pH meter) with pH 4.0 until the effluent pH was stable at 4.0, and the equilibrium was completed;

[0069] 1 g of short-petaled Trollius chinensis Bunge crude extract was weighed, added into 100 mL of acetic acid-sodium acetate buffer solution with pH 4.0, and magnetically stirred for 30 min to obtain a sample solution; the sample solution was pumped into the chromatography column at a constant flow rate of 10 mL / min; during the sample loading process, 5 mL of effluent was collected every 10 min, and when the target spot (consistent with the control) appeared in the effluent detected by thin layer chromatography (TLC), the sample loading was stopped; after the sample loading was completed, the chromatography column was washed with deionized water at a flow rate of 10 mL / min until the effluent was colorless and transparent, and the water-soluble impurities were removed; the part of the eluent was discarded, and 50% ethanol solution was used to elute at a flow rate of 8 mL / min; the eluent was collected in sections (20 mL each time), and the effective eluent was combined; the combined effective eluent was transferred to a 500 mL rotary evaporator flask, and the rotary evaporator was started with the following parameters: water bath temperature 45°C, vacuum degree 0.08 MPa, and rotation speed 55 r / min; the eluent was concentrated to a volume of 30 mL; after the concentration was completed, a small amount of deionized water (5 mL) was used to rinse the inner wall of the evaporator flask; the concentrated eluent was combined into a 50 mL freeze-drying flask, which was placed in a -80°C ultra-low temperature refrigerator for 5 h until the concentrated eluent was completely frozen; the pre-frozen freeze-drying flask was transferred to a freeze-drying machine, and the freeze-drying was performed at a cold trap temperature of -55°C and a vacuum degree of 10 Pa for 24 h; the freeze-dried powder was taken out, ground, and passed through an 80-mesh sieve to obtain the short-petaled Trollius chinensis Bunge extract.

[0070] Preparation Example 6

[0071] The preparation method of the Prinsepia uniflora extract includes the following steps:

[0072] The residue after extracting oil from Prinsepia uniflora seed is removed from the shell, impurities and un-extracted seed kernel hard block, and the loose seed meal debris is retained, dried in a 50°C air drying oven for 5h (water content is reduced to ≤8%), crushed, passed through an 80 mesh screen, and 100g of the powder collected under the screen is poured into a 2L microwave extraction beaker; deionized water is added at a solid-liquid ratio of 1:10 (g / mL), and microwave treatment is performed (microwave power is set to 250W, magnetic stirring speed is 200r / min, and the total microwave time is 60min), the mixture after microwave treatment is transferred to a 2L round-bottom flask, connected to a reflux condenser, the top of the condenser is connected to a vacuum line (connected to a vacuum pump) and a thermometer interface, the flask is placed in a constant temperature water bath, the vacuum degree is adjusted to 0.05MPa, and the water bath temperature is 40°C, and extraction is performed for 90min; after extraction, the hot filter is filtered with a 200 mesh filter cloth, the filtrate is collected in a 1L beaker, the filter residue is washed with 50mL of 40°C distilled water for 2 times, the washing liquid is combined with the filtrate, and a rotary evaporator is started (water bath temperature is 45°C, vacuum degree is 0.08MPa, and rotation speed is 60r / min); when the volume is concentrated to 1 / 4 of the original volume, the concentration is stopped, and a concentrated solution is obtained;

[0073] The concentrated solution is placed in a 5L beaker, and a constant flow pump is used to add a 95% ethanol solution to the concentrated solution at a speed of 140mL / min under magnetic stirring; after the addition is completed, stirring is continued for 10min, and the solution is placed in a 4°C refrigerator for 2h; then, the solution is filtered under reduced pressure (vacuum degree is 0.06MPa) with a 200 mesh filter cloth, the filter cake is collected and transferred to a freeze-drying bottle, and placed in a-80°C ultra-low temperature refrigerator for pre-freezing for 4h; then, the solution is transferred to a freeze dryer and freeze-dried at a cold trap temperature of-55°C and a vacuum degree of 10Pa for 24h; then, the solution is ground and passed through an 80 mesh screen to obtain the Prinsepia uniflora extract.

[0074] Preparation Example 7

[0075] The preparation method of the Ophiopogon japonicus extract includes the following steps:

[0076] Fresh Ophiopogon japonicus roots are selected, washed, dried, crushed, passed through a 20 mesh screen, and the powder collected under the screen is collected;

[0077] A 1000 mL round bottom flask was added with 500 mL of deionized water, a distillation support with a sieve plate (sieve plate aperture 5 mm) was connected, 300 g of an orchid root powder was evenly spread on the sieve plate, a layer of defatted cotton was covered on top, a condenser tube was connected in sequence, a tail pipe was connected, the tail pipe was inserted into a separatory funnel, the whole device was ensured to be sealed, after the water in the flask boiled to generate stable water vapor, the heating power was adjusted to stabilize the vapor generation rate (the drop rate of the distillate at the outlet of the condenser tube was 1-2 drops per second), the distillation time was 5 h, the distillation was stopped, the distillate was transferred to the separatory funnel, and was left to stand for 30 min; after the oil-water interface was clear, the lower layer of the crude essential oil was slowly released, the crude essential oil was taken into a conical flask, anhydrous sodium sulfate (the amount was 10% of the mass of the essential oil) was added, it was sealed and placed on a shaking bed to shake for 30 min, the dehydrated essential oil was filtered with a qualitative filter paper to remove the anhydrous sodium sulfate particles, and the preliminary purified essential oil was obtained; the preliminary purified essential oil was frozen in a refrigerator at-5 ℃ for 24 h to make the wax crystallize and precipitate, and then a pre-cooled to 0 ℃ Buchner funnel was used to perform suction filtration, and the filtrate was collected, thereby the orchid extract was obtained.

[0078] Example 1

[0079] A preparation method of a sensitive skin facial moisturizing essence, comprising the following steps:

[0080] S1, mix litchi kernel extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Prinsepia uniflora extract, deionized water, thickening agent, humectant, allantoin and butanediol, heat to 40 DEG C, stir for 8 min, get A phase; the mass ratio of litchi kernel extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Prinsepia uniflora extract, deionized water, thickening agent, humectant, allantoin and butanediol is 1.2:1.8:1.4:1.2:1.8:1.8:81.9:0.3:0.4:0.2:8;

[0081] S2, mix squalane, 5 viscosity silicone oil, emulsifier, emollient, orchid extract, heat to 70 DEG C, stir for 10 min, get B phase; the mass ratio of squalane, 5 viscosity silicone oil, emulsifier, emollient and orchid extract is 0.7:0.4:0.02:0.04:1.2;

[0082] S3, the B phase is added to the A phase, homogenously stirred at 45 DEG C and 8000 r / min for 10 min, C phase is added, homogenously stirred at 8000 r / min for 3 min, the sensitive skin facial moisturizing essence is obtained.

[0083] The C phase comprises antioxidant, preservative and (daily) essence; the mass ratio of antioxidant, preservative and (daily) essence is 3:1:2.

[0084] The mass ratio of the A phase, the B phase and the C phase is 72:17:1;

[0085] The thickening agent is selected from xanthan gum.

[0086] The humectant is selected from sodium hyaluronate.

[0087] The emulsifier is selected from PEG-10 dimethicone.

[0088] The emollient is selected from ethylhexylglycerin.

[0089] The antioxidant is selected from tocopheryl acetate.

[0090] The preservative is potassium sorbate.

[0091] Example 2

[0092] A preparation method of a sensitive skin facial moisturizing serum, comprising the following steps:

[0093] S1, take litchi kernel extract, giant salamander collagen protein freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Prinsepia uniflora extract, deionized water, thickening agent, humectant, allantoin and butanediol, heat to 45 DEG C, stir for 9 min, get A phase; the mass ratio of litchi kernel extract, giant salamander collagen protein freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Prinsepia uniflora extract, deionized water, thickening agent, humectant, allantoin and butanediol is 1.8:2.7:1.6:1.3:2.0:2.2:78.3:0.35:0.5:0.25:9;

[0094] S2, take squalane, 5 viscosity silicone oil, emulsifier, emollient, verbena extract, heat to 75 DEG C, stir for 8 min, get B phase; the mass ratio of squalane, 5 viscosity silicone oil, emulsifier, emollient and verbena extract is 0.8:0.45:0.03:0.05:1.3;

[0095] S3, the B phase is added to the A phase, and is homogenously stirred at 45 DEG C and 10000 r / min for 8 min, and the C phase is added and is homogenously stirred at 8000 r / min for 4 min, to obtain the sensitive skin facial moisturizing serum.

[0096] The C phase comprises antioxidant, preservative and (daily) essence; the mass ratio of the antioxidant, preservative and (daily) essence is 5:2:3;

[0097] The mass ratio of the A phase, the B phase and the C phase is 77:22:1.5;

[0098] The thickener is selected from carbomer.

[0099] The humectant is selected from 1,2-hexanediol.

[0100] The emulsifier is selected from PEG-3 polydimethylsiloxane.

[0101] The emollient is selected from cetearyl stearate.

[0102] The antioxidant is selected from 3-o-ethyl ascorbic acid.

[0103] The preservative is potassium sorbate.

[0104] Example 3

[0105] A method for preparing a facial moisturizing serum for sensitive skin includes the following steps:

[0106] S1. Take litchi seed extract, giant salamander collagen freeze-dried powder, black chokeberry extract, lentil seed extract, short-petaled golden lotus extract, green prickly pear fruit extract, deionized water, thickener, moisturizer, allantoin and butylene glycol, heat to 50℃, stir for 10 min to obtain phase A; the mass ratio of litchi seed extract, giant salamander collagen freeze-dried powder, black chokeberry extract, lentil seed extract, short-petaled golden lotus extract, green prickly pear fruit extract, deionized water, thickener, moisturizer, allantoin and butylene glycol is 2.4:3.6:1.8:1.4:2.2:2.6:74.7:0.4:0.6:0.3:10;

[0107] S2. Mix squalane, 5 viscosity silicone oil, emulsifier, emollient, and vetiver extract, heat to 80°C, and stir for 5 minutes to obtain phase B; the mass ratio of squalane, 5 viscosity silicone oil, emulsifier, emollient, and vetiver extract is 0.9:0.5:0.04:0.06:1.4.

[0108] S3. Add phase B to phase A, and homogenize and stir at 12000 r / min for 5 min at 45°C. Add phase C and homogenize and stir at 8000 r / min for 5 min to obtain the sensitive skin facial moisturizing essence.

[0109] Phase C comprises antioxidants, preservatives, and (daily-use) fragrances; the mass ratio of the antioxidants, preservatives, and (daily-use) fragrances is 7:3:4;

[0110] The mass ratio of phase A, phase B, and phase C is 81:27:2;

[0111] The thickener is selected from hydroxyethyl cellulose.

[0112] The humectant is selected from sodium hyaluronate.

[0113] The emulsifier is selected from PEG-10 dimethicone.

[0114] The emollient is selected from cetyl palmitate.

[0115] The antioxidant is selected from 3-o-ethyl ascorbic acid.

[0116] The preservative is potassium sorbate.

[0117] Comparative Example 1

[0118] This comparative example provides a sensitive skin facial moisturizing serum, which is only different from Example 2 in that the component does not contain achachair extract, and the reduced amount is proportionally distributed to andrewsarchus collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, prunus armeniaca extract, and sedum lineare extract, and the rest of the components and the amount remain unchanged.

[0119] Comparative Example 2

[0120] This comparative example provides a sensitive skin facial moisturizing serum, which is only different from Example 2 in that the component does not contain andrewsarchus collagen freeze-dried powder, and the reduced amount is proportionally distributed to achachair extract, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, prunus armeniaca extract, and sedum lineare extract, and the rest of the components and the amount remain unchanged.

[0121] Comparative Example 3

[0122] This comparative example provides a sensitive skin facial moisturizing serum, which is only different from Example 2 in that the component does not contain aronia melanocarpa extract, and the reduced amount is proportionally distributed to achachair extract, andrewsarchus collagen freeze-dried powder, lentil seed extract, short-petaled gypsophila extract, prunus armeniaca extract, and sedum lineare extract, and the rest of the components and the amount remain unchanged.

[0123] Comparative Example 4

[0124] This comparative example provides a sensitive skin facial moisturizing serum, which is only different from Example 2 in that the component does not contain lentil seed extract, and the reduced amount is proportionally distributed to achachair extract, andrewsarchus collagen freeze-dried powder, aronia melanocarpa extract, short-petaled gypsophila extract, prunus armeniaca extract, and sedum lineare extract, and the rest of the components and the amount remain unchanged.

[0125] Comparative Example 5

[0126] The comparative example provides a sensitive skin facial moisturizing serum, which is only different from example 2 in that the component does not contain short-petaled primula extract, and the reduced amount is proportionally distributed to litchi kernel extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, Chinese dwarf cherry extract, and sedum lineare extract, and the rest of the components and the amount remain unchanged.

[0127] Comparative example 6

[0128] The comparative example provides a sensitive skin facial moisturizing serum, which is only different from example 2 in that the component does not contain Chinese dwarf cherry extract, and the reduced amount is proportionally distributed to litchi kernel extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled primula extract, and sedum lineare extract, and the rest of the components and the amount remain unchanged.

[0129] Comparative example 7

[0130] The comparative example provides a sensitive skin facial moisturizing serum, which is only different from example 2 in that the component does not contain sedum lineare extract, and the reduced amount is proportionally distributed to litchi kernel extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled primula extract, and Chinese dwarf cherry extract, and the rest of the components and the amount remain unchanged.

[0131] Performance test

[0132] 1) Moisturizing performance test

[0133] A multi-probe skin test system is used to test the water content of the stratum corneum of the skin of the subjects with a skin moisture probe, and the high and low values of the measured values directly reflect the high and low of the water content of the stratum corneum of the skin.

[0134] Fifty healthy volunteers aged 38-48 years old, without skin diseases, no allergy to skin care products, no use of hormones or immunosuppressants in the past month, no serious systemic diseases or immune deficiencies or autoimmune diseases. Every 5 volunteers form a group, and are randomly divided into 10 groups, corresponding to the use of the serum prepared in examples 1-3 and comparative examples 1-7.

[0135] Do not touch water 2 hours before the test, and clean uniformly before the test, i.e. wipe with a dry paper towel. Sit still for 30 minutes in a laboratory with a temperature of (21±1) ℃ and a humidity of 50%±10%. The left and right forearms of each subject are marked with control and test areas, respectively, and the test skin moisture baseline value (T0) is measured, then 2.0±0.1) mg / cm 2The amount of each of the above-mentioned components was used to apply an equal amount of deionized water and serum, and the skin moisture value after 8 hours of using the serum was measured, the data was recorded and the skin water content change rate (skin improvement degree) was calculated, the average value of each group was taken and summarized, and the test results are shown in Table 1.

[0136] The calculation formula is as follows:

[0137] Skin improvement degree = (test area skin moisture value - control area skin moisture value) / control area skin moisture value x 100%

[0138] Table 1

[0139]

[0140] From Table 1, it can be seen that the serum prepared in Examples 1-3 has good moisturizing effect, can improve the water content of the stratum corneum, and achieve the effect of moisturizing the skin. The serum prepared in Comparative Examples 1-7 lacks the synergistic effect between components, resulting in a decrease in moisturizing effect compared to each example.

[0141] 2) Sensitivity skin repair performance test

[0142] Female volunteers aged 25-35 years old who met the sensitivity skin diagnosis standard were selected and randomly divided into 10 groups, 5 people in each group. The serum prepared in Examples 1-3 and Comparative Examples 1-7 was taken in an appropriate amount and evenly applied to the red and swollen parts, and then gently massaged for 3-5 min, once in the morning and once in the evening, for 28 consecutive days. During the whole process, the diet and rest should be standardized, and the affected area should not be squeezed and frequently touched. VISIA-CR was used to collect facial images before and after treatment, and skin a* value analysis was performed. The smaller the value, the lighter the skin redness. The test results are shown in Table 4.

[0143] Table 4

[0144]

[0145] From the above experimental results, it can be seen that the serum prepared in Examples 1-3 has good sensitive skin repair and soothing effect, which is better than each comparative example.

[0146] In summary, by adding litchi kernel extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lentil seed extract, short-petaled gypsophila extract, Prinsepia uniflora extract, and sedum lineare extract, the synergistic compatibility of each component has good moisturizing effect, achieves the effect of moisturizing the skin, and improves the problem of sensitive skin repair:

[0147] Litchi seed extract is rich in litchi seed polysaccharides, which can bind water molecules through hydrogen bonds to increase the water content of the skin surface layer, and promote the synthesis of lipids between keratinocytes to strengthen the skin barrier function. This property helps to alleviate the redness, tingling and other problems caused by barrier damage in sensitive skin.

[0148] Andoum Collagen Freeze-dried powder is rich in small molecular peptide active ingredients, which can penetrate into the deep layer of the skin, promote cell metabolism to improve water retention capacity, reduce water loss, maintain skin moisture, stimulate keratinocyte proliferation, strengthen skin barrier structure, indirectly improve the water retention capacity of the epidermis, improve the sensitive problems caused by dryness, accelerate the repair of damaged tissue, reduce the discomfort such as redness and tingling caused by external stimulation, balance the skin metabolism cycle, repair the sensitive problems caused by barrier damage, and enhance the tolerance of sensitive skin.

[0149] The polysaccharides in Aronia melanocarpa extract (such as pyranose, furanose, α-type glycosidic bond, β-type glycosidic bond, etc., and mostly in α configuration) have good water solubility and film-forming property, which can form a physical barrier on the skin surface to block external stimuli and prevent water loss. Polysaccharides with α-type glycosidic bond are more easily recognized by skin keratinocytes, promoting barrier regeneration. It can also regulate inflammatory pathways, relieve redness and itching of sensitive skin, reduce inflammation from the source, promote the secretion of anti-inflammatory factors, and inhibit histamine release, which can quickly relieve acute symptoms of sensitive skin. It can also extend the antioxidant effect of polysaccharides in the skin, activate the skin's own antioxidant system, and reduce the recurrence frequency of sensitive skin.

[0150] Lentil seed extract is rich in lentil polysaccharides, phytosterols, polyphenols and other active ingredients, which synergistically act on four dimensions of "barrier repair, inflammation inhibition, moisture retention and antioxidant protection" to solve the core problems of sensitive skin, such as barrier fragility, easy irritation, rapid water loss and repeated inflammation.

[0151] The short-petaled Campanula medium extract can regulate the balance of capillary vasomotion, enhance the stability of blood vessel wall, repair the vascular endothelial barrier, activate antioxidant enzymes, improve capillary dysfunction, synergistically repair the stratum corneum barrier, reduce oxidative stress, and reduce the frequency of sensitive recurrence, especially for red blood vessels and sensitive skin with redness and heat.

[0152] The improvement and repair of sensitive skin by Prinsepia uniflora fruit extract are mainly due to its rich active ingredients such as unsaturated fatty acids, phytosterols and polyphenols, which can promote ceramide synthesis, improve epidermal hydration and supplement surface lipids, repair the skin barrier and improve sensitive skin.

[0153] The stress hormone cortisol can be effectively eliminated by the extract of erigeron canus, which can induce the secretion of β-endorphin, promote nerve relaxation, and has excellent inflammation relieving ability. It can effectively inhibit the inflammatory cascade reaction, relieve skin stress, and reduce skin discomfort.

[0154] The extract of Prinsepia uniflora and the extract of Litchi chinensis can simultaneously promote the synthesis of lipids between keratinocytes, fill the gap of ceramide, and perfect the lipid structure of the stratum corneum. The freeze-dried powder of Megacalam collagen further penetrates into the superficial dermis, stimulates keratinocyte proliferation, increases the cell density of the stratum corneum, and reduces the penetration of external stimuli.

[0155] The fatty acid composition of the extract of Prinsepia uniflora is highly matched with human sebum, which can instantly supplement the missing surface lipids of sensitive skin. The extract of Lens culinaris can achieve long-term balance of the sebum film by regulating the activity of sebaceous glands and reducing the compensatory excessive secretion of sebum due to barrier damage. The two components work together to make the sebum film more stable.

[0156] The extract of Trollius nanus can improve the abnormal vasomotor function by repairing the vascular endothelial barrier and enhancing the stability of the vascular wall. At the same time, the activated antioxidant enzymes can reduce the oxidative damage of the vascular endothelium, forming a double protection from the inside to the outside with the effects of the extract of Prinsepia uniflora and the extract of Litchi chinensis on the repair of the stratum corneum barrier.

[0157] The extract of Aronia melanocarpa has excellent water solubility and film-forming properties, forming a physical barrier on the skin surface to directly block water evaporation. The extract of Litchi chinensis can bind water molecules through hydrogen bonds, and the two components work together to improve the water content of the surface layer while avoiding a heavy film feeling.

[0158] After the extract of Prinsepia uniflora and the extract of Litchi chinensis perfect the lipid structure of the stratum corneum, the extract of Lens culinaris can increase the content of epidermal mucopolysaccharides to enhance the water retention capacity of the middle layer of the skin. The three components work together to reduce the loss of water from the epidermis to the outside. After the freeze-dried powder of Megacalam collagen penetrates into the dermis, it can stimulate fibroblast metabolism and increase the synthesis of hyaluronic acid in the dermis, thereby improving the water storage capacity of the skin from the deep layer. At the same time, the stimulated keratinocyte proliferation can enhance the water absorption capacity of the epidermis, completely solving the problem of dryness and repeated problems of sensitive skin.

[0159] In addition, the extract of Aronia melanocarpa can directly regulate the inflammatory pathway and inhibit the release of histamine and other core mediators of acute irritation, quickly relieving acute discomfort such as redness and stinging. The secretion of anti-inflammatory factors promoted by it can further enhance the acute relief effect. The extract of Lens culinaris can achieve multi-dimensional anti-inflammatory by eliminating inflammatory mediators and inhibiting the inflammatory cascade reaction. The extract of Prinsepia uniflora and the extract of Trollius nanus can reduce inflammation induced by oxidative stress through antioxidant, and the components work together to reduce the frequency of inflammation recurrence and avoid repeated stimulation of sensitive skin.

[0160] The sedum extract relieves stress-induced sensitive discomfort by eliminating stress hormone cortisol and inducing secretion of anti-inflammatory neurotransmitter β-endorphin; its inhibited inflammatory cascade complements the anti-inflammatory mechanisms of the black chokeberry and the lentil seed, making anti-inflammation more comprehensive;

[0161] The black chokeberry extract prolongs the duration of antioxidant effect and can continuously remove epidermal ROS; the lentil seed extract and the greenbrier fruit extract directly neutralize free radicals through phenolic hydroxyl groups, especially for oxidative damage induced by ultraviolet light, and the three synergistically reduce epidermal oxidative stress;

[0162] The short-petaled gloxinia extract and the sedum extract can regulate cell metabolism and enhance the activity of skin's own antioxidant enzymes, and the two form an external-internal antioxidant synergy with direct antioxidant ingredients, reducing long-term damage to the barrier by oxidative stress and reducing the frequency of sensitive recurrence;

[0163] The synergistic effect of the seven extracts can simultaneously meet the specific needs of different sensitive skin types, ultimately forming a complete closed loop of protection-repair-maintenance-soothing, achieving a synergistic effect of short-term soothing and long-term repair.

[0164] The above is only the preferred embodiment of the present application, and does not limit the present application in any form. Although the present application has been disclosed as above with the preferred embodiment, it is not intended to limit the present application. Any person skilled in the art can make some changes or modifications to the above disclosed technical content without departing from the scope of the technical solution of the present application, and any equivalent embodiments with equivalent changes and modifications made on the basis of the technical essence of the present application to the above embodiments are still within the scope of the technical solution of the present application.

Claims

1. A facial moisturizing essence for sensitive skin, characterized in that, include: Phase A: Litchi seed extract, giant salamander collagen freeze-dried powder, black chokeberry extract, lentil seed extract, short-petaled golden lotus extract, green prickly pear fruit extract, deionized water, thickener, moisturizer, allantoin and butylene glycol; Phase B: Squalane, silicone oil, emulsifiers, emollients, and vetiver extract; Phase C: Antioxidants, preservatives, and (daily) fragrances.

2. The facial moisturizing essence for sensitive skin according to claim 1, characterized in that, In phase A, the mass ratio of litchi seed extract, giant salamander collagen freeze-dried powder, black chokeberry extract, lentil seed extract, short-petaled golden lotus extract, green prickly pear fruit extract, deionized water, thickener, humectant, allantoin, and butylene glycol is 1.2-2.4:1.8-3.6:1.4-1.8:1.2-1.4:1.8-2.2:1.8-2.6:74.7-81.9:0.3-0.4:0.4-0.6:0.2-0.3:8-10.

3. The facial moisturizing essence for sensitive skin according to claim 1, characterized in that, In phase B, the mass ratio of squalane, silicone oil, emulsifier, emollient and vetiver extract is 0.7-0.9:0.4-0.5:0.02-0.04:0.04-0.06:1.2-1.

4.

4. The facial moisturizing essence for sensitive skin according to claim 1, characterized in that, In phase C, the mass ratio of the antioxidant, preservative and (daily) fragrance is 3-7:1-3:2-4.

5. A facial moisturizing essence for sensitive skin according to claim 1, characterized in that, The thickener is selected from at least one of xanthan gum, carbomer, and hydroxyethyl cellulose.

6. A facial moisturizing essence for sensitive skin according to claim 1, characterized in that, The moisturizer is selected from at least one of sodium hyaluronate and 1,2-hexanediol.

7. A facial moisturizing essence for sensitive skin according to claim 1, characterized in that, The emulsifier is selected from at least one of PEG-10 polydimethylsiloxane and PEG-3 polydimethylsiloxane.

8. A facial moisturizing essence for sensitive skin according to claim 1, characterized in that, The emollient is selected from at least one of ethylhexylglycerin, cetearyl stearate, and cetearyl palmitate.

9. The method for preparing the facial moisturizing essence for sensitive skin according to any one of claims 1-8, characterized in that, Includes the following steps: S1. Take lychee seed extract, giant salamander collagen freeze-dried powder, black chokeberry extract, lentil seed extract, short-petaled golden lotus extract, green prickly pear fruit extract, deionized water, thickener, moisturizer, allantoin and butylene glycol, heat to 40-50℃, stir for 8-10 minutes to obtain phase A. S2. Mix squalane, silicone oil, emulsifier, emollient, and vetiver extract, heat to 70-80℃, and stir for 5-10 minutes to obtain phase B; S3. Add phase B to phase A, and homogenize and stir at 8000-12000 r / min for 5-10 min at 45°C. Add phase C and homogenize and stir at 8000 r / min for 3-5 min to obtain the sensitive skin facial moisturizing essence.

10. The method for preparing the facial moisturizing essence for sensitive skin according to claim 9, characterized in that, The mass ratio of phase A, phase B, and phase C is 72-81:17-27:1-2.

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