Pure natural skin care composition with effects of removing acnes and fading marks as well as preparation method and application of pure natural skin care composition

Abstract

The invention provides a pure natural skin care composition with acne removing and mark fading functions and a preparation method and application thereof, and relates to the technical field of daily necessities. The pure natural skin care composition is prepared from the following components in parts by weight: 20 to 30 parts of alfalfa extract, 10 to 20 parts of tilia latifolia flower extract, 2 to 4 parts of pachysandra repens fruit extract, 2 to 4 parts of flos eriocauli flower / stem extract, 2 to 4 parts of porphyra yezoensis polysaccharide and 2 to 4 parts of polianthes tuberosa polysaccharide. The composition disclosed by the invention is safe, mild and non-irritating, has better anti-inflammatory and acne-removing effects, is not easy to relapse, solves the problem of skin inflammation caused by acnes, lightens acne marks, does not cause skin allergy, and reduces adverse reactions in a use process.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of daily necessities, in particular to a pure natural skin care composition with acne removal and mark lightening, and a preparation method and application thereof. BACKGROUND

[0002] Acne vulgaris, also known as acne or acne, is collectively referred to as acne in medicine, which is a chronic inflammatory skin disease occurring in the sebaceous gland, and is one of the most common skin problems, which is prone to occur in adolescents during puberty. Its pathological mechanism is complex, which is closely related to factors such as excessive sebum secretion, blockage of hair follicle sebaceous duct, bacterial infection and inflammatory reaction. If not treated in time or irregularly, it will leave acne marks, acne pits and acne scars, which will have varying degrees of impact on the life, work and psychological confidence of the patient. Therefore, the treatment of acne and the repair of skin is a problem that cannot be ignored.

[0003] At present, a variety of cosmetics or skin care products with anti-acne effect have been developed for acne skin. However, these products may contain a large amount of antibiotics and other irritating ingredients to the skin, although they can quickly inhibit bacterial growth, but long-term or excessive use may lead to damage to the skin barrier function, imbalance of microecology, and even induce drug resistance, and the repair effect on pigmentation and scars left by acne is limited, and it cannot fundamentally solve the problem of acne recurrence.

[0004] Therefore, it is necessary to develop a skin care product with pure natural source, safety and no irritation, high efficiency in acne removal and mark lightening, and not easy to recur, which has practical significance. SUMMARY

[0005] Therefore, the present application provides a pure natural skin care composition with acne removal and mark lightening, and a preparation method and application thereof.

[0006] The technical scheme of the present application is as follows: The pure natural skin care composition with acne removal and mark lightening comprises, by weight: 20-30 parts of alfalfa extract, 10-20 parts of Tilia japonica extract, 2-4 parts of red-leafed acerola fruit extract, 2-4 parts of hairy vetch flower / stem extract, 2-4 parts of porphyra dentata polysaccharide and 2-4 parts of tuberose polysaccharide.

[0007] Further, the composition comprises, by weight: 25 parts of alfalfa extract, 15 parts of Tilia japonica extract, 3 parts of red-leafed acerola fruit extract, 3 parts of hairy vetch flower / stem extract, 3 parts of porphyra dentata polysaccharide and 3 parts of tuberose polysaccharide. Alternatively, the composition comprises, by weight: 20 parts of alfalfa extract, 10 parts of Tilia japonica extract, 2 parts of red-leafed acerola fruit extract, 2 parts of hairy vetch flower / stem extract, 2 parts of porphyra dentata polysaccharide and 2 parts of tuberose polysaccharide. Or, according to parts by weight, including: alfalfa extract 30 parts, broadleaf linden flower extract 20 parts, red leaf Acalypha fruit extract 4 parts, semen astragali complanati flower / stem extract 4 parts, porphyra dentata polysaccharide 4 parts, tuberose polysaccharide 4 parts.

[0008] Further, the preparation method of the alfalfa extract comprises: After the alfalfa is crushed, the extraction liquid is added, first extracted at 30-40 DEG C for 2-4h, then extracted at 50-70 DEG C for 1-2h, and finally extracted at 75-85 DEG C for 30-60min, filtered, vacuum dried to obtain the alfalfa extract; The extraction liquid is composed of polylactic acid, mannitol, polyethylene glycol and water with a mass ratio of 1-2:3-5:1-1.5:10; the mass ratio of the alfalfa to the extraction liquid is 1:8-10.

[0009] Further, the preparation method of the broadleaf linden flower extract comprises: crushing the broadleaf linden flower, adding the eutectic solvent according to the solid-liquid ratio of 1:5-8g / mL, ultrasonic low-oxygen extraction for 1-2h, filtering, freeze-drying to obtain the broadleaf linden flower extract; The eutectic solvent is betaine and lactic acid with a molar ratio of 1:1-2; the oxygen content of the ultrasonic low-oxygen is 1%-3%, the temperature is 40-50 DEG C, the frequency is 15-25 kHz, and the power is 150-250 W.

[0010] Further, the preparation method of the red leaf Acalypha fruit extract comprises: crushing the red leaf Acalypha fruit, adding water, adding compound strains for fermentation, sterilization, and vacuum concentration to obtain the red leaf Acalypha fruit extract; The solid-liquid ratio of the red leaf Acalypha fruit to water is 1:10-20g / mL; the compound strains are fermented by Lactobacillus plantarum, Lactobacillus rhamnosus and Bacillus subtilis with a mass ratio of 1:1-3:0.5-0.8, and the addition amount of the compound strains is 1%-2% of the mass of the red leaf Acalypha fruit; the fermentation is carried out at 25-30 DEG C for 24-36h.

[0011] Further, the preparation method of the semen astragali complanati flower / stem extract comprises: crushing the flower and stem parts of semen astragali complanati, adding ethanol solution according to the solid-liquid ratio of 1:8-15g / mL for extraction, filtering, and drying to obtain the semen astragali complanati flower / stem extract; The concentration of the ethanol solution is 40%-60%v / v; the extraction is carried out at 50-70 DEG C for 5-7h.

[0012] The application also provides a preparation method of a pure natural skin care composition with acne removal and scar lightening, comprising: Mixing the alfalfa extract, broadleaf linden flower extract, red-leaf acerola fruit extract, semen astragali complanati flower / stem extract, porphyra yezoensis glycan and tuberose glycan to obtain a pure natural skin care composition with acne removal and mark lightening.

[0013] The application also provides application of the pure natural skin care composition with acne removal and mark lightening in preparation of a product for inhibiting inflammatory factors.

[0014] Further, the inflammatory factors include IL-10 and IL-12.

[0015] Compared with the prior art, the application has the following beneficial effects: 1. The application extracts plants by using respective specific extraction methods to extract respective active ingredients, and the pure natural skin care composition with acne removal and mark lightening is obtained after combination, and the composition includes alfalfa extract, broadleaf linden flower extract, red-leaf acerola fruit extract and semen astragali complanati flower / stem extract, and the composition can resist inflammation and remove acne, solve the skin inflammation problem caused by acne, lighten acne marks, and is supplemented by porphyra yezoensis glycan and tuberose glycan to moisturize and relieve dry skin caused by inflammation.

[0016] 2. The pure natural skin care composition is safe, mild and non-irritating, and does not cause skin allergy and reduce adverse reactions in the use process. It can be seen that the pure natural skin care composition with acne removal and mark lightening has good industrial application prospect in the product for inhibiting inflammatory factors. DETAILED DESCRIPTION

[0017] In order to better understand the technical content of the application, the following specific examples are provided to further illustrate the application.

[0018] The experimental methods used in the embodiments of the application are conventional methods unless otherwise specified.

[0019] The materials, reagents and the like used in the embodiments of the application can be obtained from commercial channels unless otherwise specified.

[0020] The alfalfa extract of the application is derived from alfalfa, and the Latin name is Medicago sativa L. MEDICAGO SATIVA The broadleaf linden flower extract of the application is derived from broadleaf linden, and the Latin name is Tilia japonica. TILIA PLATYPHYLLOS The red-leaf acerola fruit extract of the application is derived from red-leaf acerola, and the Latin name is Malpighia emarginata DC. MALPIGHIA PUNICIFOLIA The semen astragali complanati flower / stem extract of the application is derived from semen astragali complanati, and the Latin name is Astragalus complanatus R. Br. ERIOCAULON BUERGARIANUM .

[0021] The concentration of the pure natural skin care composition with acne removal and mark lightening of the application in preparation of a product for acne removal and mark lightening is 5wt%, and the product includes but is not limited to water, milk, cream and paste.

[0022] The viable cell count of the bacterial strain of the present application is greater than 0.5 x 10 7 cfu / g.

[0023] Example 1 The pure natural skin care composition with acne removal and mark lightening of the present embodiment is composed of the following components: 25 g of alfalfa extract, 15 g of linden flower extract, 3 g of acerola cherry fruit extract, 3 g of herba leucophaeae flower / stem extract, 3 g of porphyra yezoensis polysaccharide, and 3 g of tuberose polysaccharide.

[0024] The preparation method of the alfalfa extract comprises the following steps: after the alfalfa is crushed, the crushed alfalfa is added into an extraction solution (polylactic acid, mannitol, polyethylene glycol and water with a mass ratio of 1:4:1.2:10) at a mass ratio of 1:9, and then extracted at 35℃ for 3 h, extracted at 60℃ for 1.5 h, and finally extracted at 80℃ for 45 min, filtered, and vacuum dried to obtain the alfalfa extract.

[0025] The preparation method of the linden flower extract comprises the following steps: the linden flower is crushed, and then added into a deep eutectic solvent (containing water in an amount of 50%, and betaine and lactic acid in a molar ratio of 1:2) at a solid-liquid ratio of 1:8 g / mL, extracted at 45℃ for 2 h under the conditions of 3% oxygen content, a frequency of 20 kHz, and a power of 200 W, filtered, and freeze-dried to obtain the linden flower extract.

[0026] The preparation method of the acerola cherry fruit extract comprises the following steps: the acerola cherry fruit is crushed, and then added into water at a solid-liquid ratio of 1:15 g / mL, and a composite bacterial strain (lactobacillus plantarum, lactobacillus rhamnosus and bacillus subtilis with a mass ratio of 1:2:0.7) is added in an amount of 2% of the mass of the acerola cherry fruit, and then fermented at 25℃ for 24 h, sterilized, and concentrated under reduced pressure to obtain the acerola cherry fruit extract.

[0027] The preparation method of the herba leucophaeae flower / stem extract comprises the following steps: the flower and stem parts of herba leucophaeae are crushed, and then added into a 50% v / v ethanol solution at a solid-liquid ratio of 1:10 g / mL, extracted at 60℃ for 6 h, filtered, and dried to obtain the herba leucophaeae flower / stem extract.

[0028] The preparation method of the pure natural skin care composition with acne removal and mark lightening of the present embodiment comprises the following steps: The alfalfa extract, the linden flower extract, the acerola cherry fruit extract, the herba leucophaeae flower / stem extract, the porphyra yezoensis polysaccharide and the tuberose polysaccharide are mixed and uniformly stirred to obtain the skin care composition with acne removal and mark lightening.

[0029] Example 2 The pure natural skin care composition with acne removal and mark lightening of the embodiment is composed of the following components: Medicago officinalis extract 20g, Tilia japonica extract 10g, Malpighia emarginata fruit extract 2g, Desmodium caudatum flower / stem extract 2g, Porphyra yezoensis polysaccharide 2g, Polysaccharide from tuberose 2g.

[0030] The preparation method of the Medicago officinalis extract comprises the following steps: after the Medicago officinalis is crushed, the Medicago officinalis is added into an extraction solution (polylactic acid, mannitol, polyethylene glycol and water with a mass ratio of 1:3:1:10) at a mass ratio of 1:8, and then extracted at 30 ℃ for 2 h, extracted at 50 ℃ for 1 h and finally extracted at 75 ℃ for 30 min, filtered, vacuum dried, and the Medicago officinalis extract is obtained.

[0031] The preparation method of the Tilia japonica extract comprises the following steps: the Tilia japonica is crushed, added into a deep eutectic solvent (containing 50% of water, and betaine and lactic acid at a molar ratio of 1:1) at a solid-liquid ratio of 1:5 g / mL, extracted at 10 ℃ for 1 h under the condition of 3% of oxygen content, 15 kHz of frequency and 150 W of power, filtered, freeze-dried, and the Tilia japonica extract is obtained.

[0032] The preparation method of the Malpighia emarginata fruit extract comprises the following steps: the Malpighia emarginata fruit is crushed, added into water at a solid-liquid ratio of 1:10 g / mL, and then added with a composite strain (Lactobacillus plantarum, Lactobacillus rhamnosus and Bacillus subtilis at a mass ratio of 1:1:0.5) at an amount of 1% of the Malpighia emarginata fruit, fermented at 25 ℃ for 24 h, sterilized, and concentrated under reduced pressure, and the Malpighia emarginata fruit extract is obtained.

[0033] The preparation method of the Desmodium caudatum flower / stem extract comprises the following steps: the flower and stem parts of Desmodium caudatum are crushed, added into 40% v / v ethanol solution at a solid-liquid ratio of 1:8 g / mL, extracted at 50 ℃ for 5 h, filtered, dried, and the Desmodium caudatum flower / stem extract is obtained.

[0034] The preparation method of the pure natural skin care composition with acne removal and mark lightening of the embodiment comprises the following steps: The Medicago officinalis extract, the Tilia japonica extract, the Malpighia emarginata fruit extract, the Desmodium caudatum flower / stem extract, the Porphyra yezoensis polysaccharide and the Polysaccharide from tuberose are mixed and stirred uniformly, and the skin care composition with acne removal and mark lightening is obtained.

[0035] Example 3 The pure natural skin care composition with acne removal and mark lightening of the embodiment is composed of the following components: Medicago officinalis extract 30g, Tilia japonica extract 20g, Malpighia emarginata fruit extract 4g, Desmodium caudatum flower / stem extract 4g, Porphyra yezoensis polysaccharide 4g, Polysaccharide from tuberose 4g.

[0036] The preparation method of alfalfa extract includes the following steps: after pulverizing alfalfa, add it to the extraction solution (polylactic acid, mannitol, polyethylene glycol and water in a mass ratio of 1:5:1.5:10) at a mass ratio of 1:10. First, extract at 40℃ for 4 hours, then extract at 70℃ for 2 hours, and finally extract at 85℃ for 60 minutes. Filter and vacuum dry to obtain alfalfa extract.

[0037] The preparation method of broadleaf linden flower extract includes the following steps: pulverize broadleaf linden flowers, add them to a eutectic solvent (betaine and lactic acid with a water content of 50% and a molar ratio of 1:2) at a material-to-liquid ratio of 1:8 g / mL, extract for 2 hours at an oxygen content of 3%, a temperature of 50℃, a frequency of 25kHz, and a power of 250W, filter, and freeze-dry to obtain broadleaf linden flower extract.

[0038] The preparation method of the extract of *Gynostemma pentaphyllum* includes the following steps: crush *Gynostemma pentaphyllum* into powder, add water at a solid-liquid ratio of 1:20 g / mL, add compound microbial strains (*Lactobacillus plantarum*, *Lactobacillus rhamnosus* and *Bacillus subtilis* in a mass ratio of 1:3:0.8), the amount of compound microbial strains added is 2% of the mass of *Gynostemma pentaphyllum*, ferment at 30℃ for 36 h, sterilize, concentrate under reduced pressure to obtain the extract of *Gynostemma pentaphyllum*.

[0039] The preparation method of Eriocaulon flower / stem extract includes the following steps: crush the flowers and stems of Eriocaulon, add them to a 60% v / v ethanol solution at a solid-liquid ratio of 1:15 g / mL, extract at 70℃ for 7 h, filter, and dry to obtain Eriocaulon flower / stem extract.

[0040] The preparation method of the all-natural skin care composition for acne removal and fading of acne scars in this embodiment includes: Alfalfa extract, broadleaf linden flower extract, red-leaf golden tamarisk fruit extract, Eriocaulon flower / stem extract, Porphyra stripe polysaccharide and tuberose polysaccharide are mixed and stirred evenly to obtain a skin care composition for acne removal and fading of acne scars.

[0041] Comparative Example 1 The composition of this comparative example differs from that of Example 1 in that it only includes extracts of broadleaf linden flowers, extracts of red-leaf golden tamarisk fruit, extracts of Eriocaulon buergerianum flowers / stems, polysaccharides of Porphyra yezoensis, and polysaccharides of Tuberose. The amount of alfalfa extract missing is allocated to the corresponding components according to the proportions of the other substances; otherwise, it is the same as in Example 1.

[0042] Comparative Example 2 The composition of this comparative example differs from that of Example 1 in that it only includes alfalfa extract, redbud fruit extract, Eriocaulon flower / stem extract, Porphyra yezoensis polysaccharide, and tuberose polysaccharide. The amount of Tilia amurensis flower extract missing is allocated to the corresponding components according to the proportions of the other substances; otherwise, it is the same as in Example 1.

[0043] Comparative Example 3 The composition of this comparative example differs from that of Example 1 in that it only includes alfalfa extract, broadleaf linden flower extract, eriocaulon flower / stem extract, Porphyra yezoensis polysaccharide, and tuberose polysaccharide. The amount of the missing *Gynostemma pentaphyllum* extract is allocated to the corresponding components according to the proportions of the other substances; otherwise, it is consistent with Example 1.

[0044] Comparative Example 4 The composition of this comparative example differs from that of Example 1 in that it only includes alfalfa extract, broadleaf linden flower extract, red-leaf golden tamarisk fruit extract, striped laver polysaccharide, and tuberose polysaccharide. The amount of missing Eriocaulon flower / stem extract is allocated to the corresponding components according to the proportions of the other substances; otherwise, it is consistent with Example 1.

[0045] Comparative Example 5 The composition of this comparative example differs from that of Example 1 in that it only includes alfalfa extract, broadleaf linden flower extract, red-leaf golden tamarisk fruit extract, and Eriocaulon flower / stem extract. The amounts of Porphyra yezoensis polysaccharide and tuberose polysaccharide that are missing are allocated to the corresponding components according to the proportions of other substances; otherwise, it is the same as in Example 1.

[0046] Experimental Example 1 The all-natural skin care compositions for acne removal and fading of acne scars prepared in Examples 1-3 and Comparative Examples 1-5 were subjected to antibacterial tests. Methods: First, sterile circular filter paper with a diameter of 6 mm was immersed in the all-natural skin care compositions for acne removal and fading of acne scars prepared in Examples 1-3 and Comparative Examples 1-5, respectively. After soaking for 30 minutes, the samples were removed, air-dried, and drug sensitivity tablets were obtained. A sterile inoculation loop was used to pick up bacteria from Propionibacterium acnes colonies and placed them in sterile physiological saline to adjust the turbidity to 0.5 MCF. Then, a sterile cotton swab was used to evenly spread the bacterial solution on the surface of an anaerobic brain and heart infusion plate. After drying for 5 minutes, the drug sensitivity tablets from Examples 1-3 and Comparative Examples 1-5 were respectively attached to the plates and placed in an anaerobic jar. The plates were incubated at a constant temperature of 37°C for 48 hours. After removal, the diameter of the inhibition zone of the drug sensitivity tablet was measured using the cross-sectional method. The diameter of the inhibition zone was the measured diameter minus 6 mm. In addition, add 20 mL of nutrient culture medium to a sterile petri dish, blow off any condensation on the surface, and then use a sterile cotton swab to take 10 mL of the culture medium. 5CFU / mL Staphylococcus aureus bacterial suspension was evenly spread on the surface of the agar plate, ensuring that no liquid flowed onto the plate. Then, the drug sensitivity test strips from Examples 1-3 and Comparative Examples 1-5 were applied to the plates, and the plates were incubated at 37°C for 24 hours. The diameter of the inhibition zone was measured using the cross-sectional method. The results are shown in Table 1.

[0047] Table 1

[0048] As shown in Table 1, the compositions prepared in Examples 1-3 of this invention have good inhibitory effects on Propionibacterium acnes and Staphylococcus aureus, effectively inhibiting bacteria. Data from Comparative Examples 1-5 show that the antibacterial effect decreases if any component of the composition is missing, indicating that the components work synergistically.

[0049] Experimental Example 2 The anti-inflammatory effects of the all-natural skin care compositions for acne removal and fading of blemishes prepared in Examples 1-3 and Comparative Examples 1-5 were tested. Methods: Blood samples were mixed with an equal proportion of hematologic separation solution and centrifuged at 20°C for 40 min. Human peripheral blood mononuclear cell layer was removed and the cells were washed with buffer solution. Then, the cells were suspended in RPMI-1640 medium. CD14+ mononuclear cells from human peripheral blood mononuclear cells were purified using CD14+ microspheres (MiniMACS system). Cells were stimulated with cytokine IL-4 and growth hormone GM-CSF to induce differentiation into dendritic cells. After differentiation, the dendritic cells were collected and seeded into well plates. The all-natural skin care compositions for acne removal and fading of acne scars provided in Examples 1-3 and Comparative Examples 1-5 were added to well plates at a concentration of 2 μg / mL. A blank control group (with an equal volume of culture medium) was also set up. The cells were co-cultured at 37°C and 5% CO2 for 48 h. After the culture was completed, the cell culture supernatant was collected.

[0050] The levels of interleukins IL-10 and IL-12 in the supernatant were detected by enzyme-linked immunosorbent assay (ELISA). The level of anti-inflammatory cytokine IL-10 in the blank control group was defined as 0, and the level of pro-inflammatory cytokine IL-12 was defined as 100%.

[0051] The test results are shown in Table 2.

[0052] Table 2

[0053] As can be seen from Table 2, the all-natural skin care compositions for acne removal and fading of acne scars prepared in Examples 1-3 of this invention have good anti-inflammatory effects.

[0054] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A pure natural skin care composition for acne removal and fading of acne scars, characterized in that, By weight, it includes: 20-30 parts of alfalfa extract, 10-20 parts of broadleaf linden flower extract, 2-4 parts of red-leaf golden tiger tail fruit extract, 2-4 parts of Eriocaulon flower / stem extract, 2-4 parts of Porphyra stripes polysaccharide, and 2-4 parts of tuberose polysaccharide.

2. The all-natural skin care composition for acne removal and fading of acne scars as described in claim 1, characterized in that, By weight, it includes: 25 parts alfalfa extract, 15 parts broadleaf linden flower extract, 3 parts red-leaf golden tiger tail fruit extract, 3 parts eriocaulon flower / stem extract, 3 parts striped laver polysaccharide, and 3 parts tuberose polysaccharide. Alternatively, by weight, it includes: 20 parts alfalfa extract, 10 parts broadleaf linden flower extract, 2 parts red-leaf golden tiger tail fruit extract, 2 parts eriocaulon flower / stem extract, 2 parts striped laver polysaccharide, and 2 parts tuberose polysaccharide. Alternatively, by weight, it includes: 30 parts alfalfa extract, 20 parts broadleaf linden flower extract, 4 parts red-leaf golden tiger tail fruit extract, 4 parts eriocaulon flower / stem extract, 4 parts striped laver polysaccharide, and 4 parts tuberose polysaccharide.

3. The all-natural skin care composition for acne removal and fading of acne scars as described in claim 1, characterized in that, The preparation method of the alfalfa extract includes: After crushing the alfalfa, add it to the extract. First, extract at 30-40℃ for 2-4 hours, then at 50-70℃ for 1-2 hours, and finally at 75-85℃ for 30-60 minutes. Filter and vacuum dry to obtain alfalfa extract. The extract is composed of polylactic acid, mannitol, polyethylene glycol and water in a mass ratio of 1-2:3-5:1-1.5:10; the mass ratio of alfalfa to the extract is 1:8-10.

4. The all-natural skin care composition for acne removal and fading of acne scars as described in claim 1, characterized in that, The preparation method of the broadleaf linden flower extract includes: crushing the broadleaf linden flowers, adding them to a eutectic solvent at a material-to-liquid ratio of 1:5-8 g / mL, performing ultrasonic low-oxygen extraction for 1-2 hours, filtering, and freeze-drying to obtain the broadleaf linden flower extract. The eutectic solvent is betaine and lactic acid in a molar ratio of 1:1-2; the ultrasonic hypoxia has an oxygen content of 1%-3%, a temperature of 40-50℃, a frequency of 15-25kHz, and a power of 150-250W.

5. The all-natural skin care composition for acne removal and fading of acne scars as described in claim 1, characterized in that, The preparation method of the red-leaf golden tiger tail fruit extract includes: crushing the red-leaf golden tiger tail fruit, adding water, adding compound bacteria for fermentation, sterilizing, and concentrating under reduced pressure to obtain the red-leaf golden tiger tail fruit extract. The solid-liquid ratio of the red-leaf golden tiger tail fruit to water is 1:10-20 g / mL; the compound microbial strain is Lactobacillus plantarum, Lactobacillus rhamnosus, and Bacillus subtilis fermented in a mass ratio of 1:1-3:0.5-0.8, and the amount of compound microbial strain added is 1%-2% of the mass of the red-leaf golden tiger tail fruit; the fermentation is carried out at 25-30℃ for 24-36 hours.

6. The all-natural skin care composition for acne removal and fading of acne scars as described in claim 1, characterized in that, The preparation method of the Eriocaulon flower / stem extract includes: pulverizing the flowers and stems of Eriocaulon, adding them to an ethanol solution at a solid-liquid ratio of 1:8-15 g / mL for extraction, filtering, and drying to obtain the Eriocaulon flower / stem extract. The concentration of the ethanol solution is 40%-60% v / v; the extraction is performed at 50-70℃ for 5-7 hours.

7. A method for preparing the all-natural skin care composition for acne removal and fading acne scars according to any one of claims 1-6, characterized in that, include: A mixture of alfalfa extract, linden flower extract, redbud fruit extract, Eriocaulon flower / stem extract, Porphyra yezoensis polysaccharide, and tuberose polysaccharide yields a pure natural skin care composition with acne-reducing and blemish-fading properties.

8. The use of the all-natural skin care composition with acne-reducing and acne-fading properties according to any one of claims 1-6 in the preparation of a product that inhibits inflammatory factors.

9. The application as described in claim 8, characterized in that, The inflammatory factors include IL-10 and IL-12.

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