Cell reaction bag with double-gas-path ventilation device

Abstract

The invention provides a cell reaction bag with a double-gas-path ventilation device, the cell reaction bag comprises a reaction bag body, a ventilation device and a stirring device, the ventilation device comprises a ventilation disc, the ventilation disc is provided with a first hole group and a second hole group, and the ventilation disc is internally provided with a first channel and a second channel which are respectively communicated with the first hole group and the second hole group; the bottom of the ventilation disc is provided with a first air inlet and a second air inlet which are communicated with the first channel and the second channel respectively. According to the ventilation device disclosed by the invention, two groups of holes with different sizes and corresponding channels are arranged, so that double-gas-path ventilation is formed, different gases can be respectively introduced, large bubbles are generated on the outer side of the ventilation disc, and micro bubbles are generated on the inner side of the ventilation disc; the gas dissolving efficiency is improved, uniform gas distribution is ensured, and uniform growth of cells is facilitated; different requirements on gases such as oxygen and carbon dioxide in the cell culture process can be accurately controlled, and a more suitable gas environment is provided for cell growth.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell culture equipment, in particular to a cell reaction bag with a double-gas-path aeration device. BACKGROUND

[0002] With the rapid development of the biopharmaceutical industry, cell culture technology, as one of the core links of biopharmaceutical production, its improvement and innovation of the culture equipment are particularly important. Cell reaction bag, as a kind of disposable cell culture device, has been widely used in the field of biopharmaceuticals due to its convenience, no need for cleaning and sterilization, and reduced risk of cross contamination.

[0003] In the process of cell culture, the aeration system is a key component to ensure the supply of oxygen required for cell growth. However, the existing cell reaction bag has the following shortcomings: Firstly, the aeration system of the existing cell reaction bag is mostly single aeration mode, lacking a simple design scheme that can provide both micro-bubbles and macro-bubbles, making it difficult to meet the differentiated needs of cell culture at different stages in terms of bubble size and aeration volume; Secondly, the integration of the aeration system and the stirring system is not high, making it difficult to achieve the synergistic effect of aeration and stirring, affecting the gas mass transfer efficiency; Thirdly, if the gas introduced into the reaction bag is contaminated with microorganisms, it will affect the quality of cell culture, and the existing cell reaction bag has deficiencies in gas purification treatment.

[0004] Therefore, it is urgent to develop a cell reaction bag with simple structure, multiple aeration modes, high integration with stirring system and gas purification function to meet the efficient and flexible needs of cell culture equipment in biopharmaceutical production. SUMMARY

[0005] In view of the above-mentioned shortcomings of the prior art, the present application aims to provide a cell reaction bag with a double-gas-path aeration device to solve the problems of single aeration system in the existing reaction bag, low integration of the aeration system and the stirring system, and contamination risk of the introduced gas.

[0006] To achieve the above-mentioned purposes and other related purposes, the present application provides a cell reaction bag with a double-gas-path aeration device, comprising a reaction bag body, an aeration device and a stirring device; The reaction bag body has an internal space for containing cell culture solution, providing a place for cell culture; The ventilation device comprises a ventilation disc arranged at the bottom of the inner space of the reaction bag body; two groups of holes are arranged on the ventilation disc, namely a first hole group and a second hole group; a first channel and a second channel are arranged in the ventilation disc and are in communication with the first hole group and the second hole group respectively; a first air inlet and a second air inlet are arranged at the bottom of the ventilation disc and are in communication with the first channel and the second channel respectively; the first air inlet and the second air inlet are connected with a first connecting pipeline and a second connecting pipeline respectively; the first connecting pipeline and the second connecting pipeline are connected with a first air inlet pipeline and a second air inlet pipeline outside respectively. The stirring device comprises a stirring shaft and a paddle; the upper end of the stirring shaft is connected with an external driving mechanism through a sealing bearing; the lower end of the stirring shaft extends into the reaction bag body and is rotationally matched with the ventilation disc.

[0007] As a preferred embodiment, the first hole group is located at the center of the ventilation disc, and the second hole group surrounds the first hole group; the diameter of the holes in the first hole group is smaller than the diameter of the holes in the second hole group.

[0008] Further, the ventilation disc is circular, the first channel and / or the second channel are composed of a plurality of annular grooves arranged in a concentric manner, and a slot is arranged between adjacent annular grooves for communication.

[0009] Further, the holes of the first hole group and / or the second hole group are distributed in a concentric circular manner.

[0010] Further, the holes of the first hole group are micro-bubble holes with a diameter of 100-300 μm; the holes of the second hole group are large-bubble holes.

[0011] As a preferred embodiment, a disposable plasma generator is installed on the first air inlet pipeline and / or the second air inlet pipeline.

[0012] Specifically, the disposable plasma generator comprises a high-voltage electrode copper pipe, a ground electrode copper rod, an electrode fixing sheet and a shell; two electrode fixing sheets are arranged at the two ends of the high-voltage electrode copper pipe respectively; the two ends of the ground electrode copper rod are fixed at the centers of the electrode fixing sheets respectively; a through hole is further arranged on the electrode fixing sheet; the shell is arranged outside the high-voltage electrode copper pipe; electrode insertion holes are arranged at the two ends of the shell and are electrically connected with the two ends of the ground electrode copper rod respectively.

[0013] By inserting the electrode of the external controller into the electrode insertion hole of the disposable plasma generator, plasma can be excited in the air passing through this place, so that the active substances in the plasma state and the generated micro-bubbles are introduced into the culture medium together, thereby achieving the effect of promoting high expression of cells and stabilizing cell viability.

[0014] Further, the distance between the outlet of the disposable plasma generator and the liquid outlet connector is not more than 200 mm, so that the plasma can contact the culture medium as soon as possible after being excited, stimulate the expression of cells, and avoid quenching during air transmission.

[0015] As a preferred embodiment, air filters are arranged at the air inlet ends of the first and second air inlet pipelines, so that the air entering the pipelines is filtered to remove particulate matter and achieve sterilization.

[0016] As a preferred embodiment, a fixing connector is arranged at the center of the aeration disc, and the lower end of the stirring shaft is rotatably connected to the fixing connector through a bearing assembly.

[0017] Further, the bottom of the reaction bag body is provided with a liquid outlet connector, and the first and second connecting pipelines are connected to the first and second air inlet pipelines outside the reaction bag body through the liquid outlet connector; a liquid outlet is arranged on the liquid outlet connector and connected to a liquid outlet pipeline outside the reaction bag body; the fixing connector and the liquid outlet connector are connected through vertically arranged stand columns, and a liquid outlet channel is formed between the stand columns and connected to the liquid outlet.

[0018] The liquid outlet connector and the fixing connector are connected through the stand columns, and the gap between the stand columns can be used for liquid flow, ensuring the normal liquid outlet of the liquid outlet on the liquid outlet connector. The bubble function and the liquid outlet function are ingeniously integrated at the bottom of the reaction bag, ensuring that aeration and liquid outlet can be independently performed at the bottom, which not only improves the efficiency of gas exchange and liquid management during cell culture, but also optimizes the operation process of cell culture and reduces the risk of operation failure caused by functional conflicts.

[0019] Further, the first and second connecting pipelines are S-shaped, and one end of each of the first and second connecting pipelines is connected to the first and second air inlets, respectively, and the other end of each of the first and second connecting pipelines is connected to the first and second air inlet pipelines outside the reaction bag body through the channel in the liquid outlet connector. The S-shaped first and second connecting pipelines are convenient to connect to the liquid outlet connector from the gap between the stand columns, and are convenient to manufacture, disassemble and assemble.

[0020] Further, the bearing assembly includes a bearing outer tube, a bearing inner clamping piece and a bearing, a third annular groove is arranged on the fixing connector, the bearing inner clamping piece is connected to the center of the fixing connector, the lower end of the bearing outer tube is rotatably connected to the third annular groove, and the upper end of the bearing outer tube is connected to the lower end of the stirring shaft; the bearing is sleeved between the bearing inner clamping piece and the bearing outer tube.

[0021] Further, the bearing outer tube is detachably connected with the stirring shaft. Specifically, the bearing outer tube and the stirring shaft are detachably connected through a connecting piece, the lower end of the stirring shaft is provided with a mounting groove matched with the connecting piece, the stirring shaft and the connecting piece are provided with a first insertion hole penetrating therethrough, and the stirring shaft and the connecting piece are detachably connected through a first insertion pin; the bearing outer tube and the connecting piece are provided with a second insertion hole penetrating therethrough, and the bearing outer tube and the connecting piece are detachably connected through a second insertion pin.

[0022] As a preferred embodiment, the paddle is a fan-shaped paddle, and the coverage area of the fan-shaped paddle is greater than the upper surface area of the aeration disc.

[0023] As a preferred embodiment, the top of the reaction bag body is provided with a liquid inlet pipeline, the lower end of the liquid inlet pipeline extends into the reaction bag body and extends to the bottom of the reaction bag body; and the middle pipeline section of the liquid inlet pipeline is fixed to the side wall of the reaction bag body through a double-end inner extension pipe clamp. By extending the liquid inlet pipeline from the top to the bottom of the reaction bag, when the culture medium and the inoculated cells are input, the problems such as foaming of the culture medium and death of the cells under the impact force can be avoided.

[0024] Further, the top of the reaction bag body is further provided with a large-flow pipeline and other pipelines.

[0025] As a preferred embodiment, the side wall of the reaction bag body is further provided with a sampling pipeline assembly, a pH electrode connector, a dissolved oxygen electrode connector and a temperature control pipeline assembly.

[0026] Further, the side wall of the reaction bag body is further provided with at least two corrugated pipe pipeline assembly interfaces.

[0027] As described above, the cell reaction bag with a double-air-path aeration device has the following beneficial effects: 1. The aeration device of the present application forms a double-air-path aeration by setting two groups of holes with different sizes and corresponding channels, can respectively pass in different gases, and can produce large bubbles on the outside and micro-bubbles on the inside of the aeration disc; not only improves the gas dissolution efficiency, ensures the uniform distribution of the gas, and is beneficial to the uniform growth of the cells; but also can accurately control the different needs of oxygen, carbon dioxide and other gases in the cell culture process, and provide a more suitable gas environment for cell growth.

[0028] 2. The stirring device and the aeration device are designed in an integrated manner, and the two devices work together to promote the diffusion of the gas in the culture solution during the stirring process, so that the cells are in full contact with the culture solution and the gas, the absorption and metabolic efficiency of the cells to the nutrients are improved, and the cell culture effect is improved.

[0029] 3. By installing a disposable plasma generator on the air inlet pipeline, the air passing through the plasma generator can be excited to plasma, so that the active substances in the plasma state and the microbubbles generated together are introduced into the culture medium, thereby achieving the effects of promoting cell high expression and stabilizing cell activity.

[0030] 4. The detachable electrode connection mode of the external controller and the disposable plasma generator ensures flexible control, realizes the disposable use of the generator, ensures complete isolation of the plasma generator from the external environment, avoids cross-contamination risk, meets the strict sterile requirements of cell culture, and balances economy and safety.

[0031] 5. The high air flow rate design of the disposable plasma generator combined with the short distance transmission path ensures sufficient gas supply and maximally reduces the loss of plasma in the transmission process, thereby ensuring that the plasma of sufficient concentration reaches the cell culture environment.

[0032] 6. By extending the liquid inlet pipeline from the top to the bottom of the reaction bag, the problems of culture medium foaming and cell death caused by liquid impact force can be avoided when the culture medium and cells are inoculated. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1 FIG. 1 is a structural schematic diagram of a cell reaction bag with a double-air-path ventilation device disclosed in Embodiment 1 of the present application.

[0034] Figure 2 FIG. 2 is a cross-sectional schematic diagram of the cell reaction bag with the double-air-path ventilation device disclosed in Embodiment 1 of the present application.

[0035] Figure 3 FIG. 3 is a structural schematic diagram of the ventilation device disclosed in Embodiment 1 of the present application. Figure 2 FIG. 4 is an enlarged schematic diagram of position A in FIG. 3.

[0036] Figure 4 FIG. 5 is a structural schematic diagram of the ventilation device disclosed in Embodiment 1 of the present application.

[0037] Figure 5 FIG. 6 is a disassembled schematic diagram of the ventilation device disclosed in Embodiment 1 of the present application.

[0038] Figure 6 FIG. 7 is a structural schematic diagram of a cell reaction bag with a double-air-path ventilation device disclosed in Embodiment 2 of the present application.

[0039] Figure 7 FIG. 8 is a structural schematic diagram of a disposable plasma generator disclosed in Embodiment 2 of the present application.

[0040] Figure 8 FIG. 9 is a cross-sectional schematic diagram of the disposable plasma generator disclosed in Embodiment 2 of the present application.

[0041] Figure 9 Figure 2 is a disassembled view of the disposable plasma generator disclosed in Example 2 of the present application.

[0042] Element Number Description 100, reaction bag body; 110, liquid inlet pipeline; 120, double-end inner extension pipe clamp; 130, large flow pipeline; 140, sampling pipeline assembly; 150, pH electrode connector; 160, dissolved oxygen electrode connector; 170, temperature control pipeline assembly; 180, corrugated pipe pipeline assembly interface; 200, aeration device; 210, aeration disc; 211, first hole group; 212, second hole group; 213, first channel; 214, second channel; 215, first annular groove; 216, second annular groove; 217, slotted; 218, first air inlet; 220, first connecting pipeline; 230, second connecting pipeline; 240, fixed connector; 241, third annular groove; 250, stand; 251, liquid outlet channel; 260, liquid outlet connector; 261, liquid outlet; 270, first air inlet pipeline; 280, second air inlet pipeline; 300, stirring device; 310, stirring shaft; 320, paddle; 330, driving mechanism; 340, bearing assembly; 341, bearing outer tube; 342, bearing inner clamp; 343, connecting piece; 344, first jack; 345, second jack; 400, disposable plasma generator; 410, shell; 420, high-voltage electrode copper pipe; 430, ground electrode copper rod; 440, electrode fixing sheet; 450, electrode jack. DETAILED DESCRIPTION

[0043] The present application is herein described, by way of example only, with reference to certain embodiments thereof. It is to be understood that variations and modifications will be apparent to those skilled in the art and that the scope of the application encompasses all such obvious variations and modifications. Where specific details are given, it is to be understood that this is for the purpose of illustration only and that the present application is not limited thereto.

[0044] Example 1 Please refer to Figures 1-2 The present embodiment provides a cell reaction bag with a double-air-path aeration device, which comprises a reaction bag body 100, an aeration device 200, and a stirring device 300.

[0045] The reaction bag body 100 has a bottom, a top, and a sidewall, so that an internal space for accommodating cell culture solution is formed inside, providing a place for cell culture.

[0046] Reference Figures 3-5The ventilation device 200 comprises a ventilation disc 210, which is circular and composed of two upper and lower discs, and is located at the bottom of the inner space of the reaction bag body 100. Two groups of holes are formed on the upper surface of the ventilation disc 210, which are a first hole group 211 and a second hole group 212. The first hole group 211 is located at the center of the ventilation disc 210, and the second hole group 212 is arranged around the outer periphery of the first hole group 211. The holes in the first hole group 211 are micro-bubble holes with a diameter of 100-300 μm, and the holes in the second hole group 212 are macro-bubble holes with a diameter larger than that of the holes in the first hole group 211. The holes in the first hole group 211 and the second hole group 212 are arranged in a concentric circular manner, which can ensure that the gas enters the inner space of the reaction bag body uniformly.

[0047] The ventilation disc 210 is internally provided with a first channel 213 and a second channel 214, and the first hole group 211 and the second hole group 212 are vertically communicated with the first channel 213 and the second channel 214, respectively. The first channel 213 is composed of a plurality of first annular grooves 215 arranged at intervals in a concentric manner, and the slits 217 for communication are formed between adjacent first annular grooves 215. This structure design can make the gas uniformly distributed in the first channel 213, so as to form micro-bubbles with uniform size through the first hole group 211. The second channel 214 is a second annular groove 216 surrounding the outermost first annular groove 215, which is used for conveying gas to the second hole group 212 to form larger bubbles.

[0048] The bottom of the ventilation disc 210 is provided with a first gas inlet 218 and a second gas inlet, which are communicated with the first channel 213 and the second channel 214, respectively. The first gas inlet 218 and the second gas inlet are connected with a first connecting pipeline 220 and a second connecting pipeline 230, respectively. The first connecting pipeline 220 and the second connecting pipeline 230 are connected with a first gas inlet pipeline 270 and a second gas inlet pipeline 280 outside, respectively. The gas inlet end (i.e. the top end) of the first gas inlet pipeline 270 and the second gas inlet pipeline 280 is provided with an air filter (not shown in the figure), and the air entering the pipeline is filtered by the filter to achieve the effects of removing particulate matter and achieving sterility.

[0049] The center of the aeration disc 210 is provided with a fixing joint 240, and the bottom of the reaction bag body 100 is provided with a liquid outlet joint 260, and a liquid outlet 261 is formed in the liquid outlet joint 260, and the liquid outlet joint 260 is connected with an external liquid outlet pipeline; the fixing joint 240 and the liquid outlet joint 260 are connected through the stand columns 250 arranged at intervals, and the stand columns 250 form a liquid outlet channel 251 communicating with the liquid outlet 261, so as to facilitate the discharge of the culture solution. The design of the stand column on the liquid outlet joint 260 ingeniously solves the conflict problem of the bubble function and the liquid discharge function when they are implemented at the bottom. The unique structure ensures that the aeration and the liquid discharge can be independently carried out at the bottom, not only improves the efficiency of gas exchange and liquid management in the cell culture process, but also optimizes the operation process of cell culture, and reduces the risk of operation failure caused by function conflict.

[0050] The first connecting pipeline 220 and the second connecting pipeline 230 are respectively connected with the first gas inlet pipeline 270 and the second gas inlet pipeline 280 outside through the liquid outlet joint 260. One end of the first connecting pipeline 220 and the second connecting pipeline 230 is respectively connected with the first gas inlet 218 and the second gas inlet 228, and the other end of the first connecting pipeline 220 and the second connecting pipeline 230 is respectively connected with the first gas inlet pipeline 270 and the second gas inlet pipeline 280 outside through the channel in the liquid outlet joint 260. The first connecting pipeline 220 and the second connecting pipeline 230 are both S-shaped pipes, which are convenient for connecting to the liquid outlet joint 260 from the gap between the stand columns, and are also convenient for manufacturing, dismounting and mounting.

[0051] The stirring device 300 comprises a stirring shaft 310 and a paddle 320. The upper end of the stirring shaft 310 is connected with an external driving mechanism 330 through a sealing bearing, and the lower end of the stirring shaft 310 extends into the reaction bag body 100 and is rotationally matched with the aeration disc 210. The stirring shaft 310 drives the paddle 320 to rotate, so as to fully mix the culture solution in the reaction bag, improve the mass transfer efficiency of the gas, and promote the growth of cells.

[0052] The paddle 320 is a fan-shaped paddle 320, and the coverage area of the fan-shaped paddle 320 is greater than the upper surface area of the aeration disc 210. In the stirring process, the culture solution can be more fully and uniformly stirred, the gas in the culture solution can be promoted to diffuse, the cells can be fully contacted with the culture solution and the gas, the absorption and metabolic efficiency of the cells to the nutrients can be improved, and thus the cell culture effect can be significantly improved.

[0053] The lower end of the stirring shaft 310 is rotationally matched with the fixing joint 240 through a bearing assembly 340. Referring to Figure 3The bearing assembly 340 comprises a bearing outer tube 341, a bearing inner card 342 and a bearing. The fixing joint 240 is provided with a third annular groove 241, the bearing inner card 342 is connected to the center of the fixing joint 240, the lower end of the bearing outer tube 341 is rotatably connected in the third annular groove 241, and the upper end of the bearing outer tube 341 is connected with the lower end of the stirring shaft 310; the bearing is sleeved between the bearing inner card 342 and the bearing outer tube 341.

[0054] The bearing outer tube 341 is detachably connected with the stirring shaft 310. The bearing outer tube 341 and the stirring shaft 310 are detachably connected through a connecting piece 343. The lower end of the stirring shaft 310 is provided with a mounting groove matched with the connecting piece 343, the stirring shaft 310 and the connecting piece 343 are provided with a first insertion hole 344 penetrating through, and the stirring shaft 310 and the connecting piece 343 are detachably connected through a first insertion pin; the bearing outer tube 341 and the connecting piece 343 are provided with a second insertion hole 345 penetrating through, and the bearing outer tube 341 and the connecting piece 343 are detachably connected through a second insertion pin. The detachable design facilitates the installation, maintenance and cleaning of the equipment.

[0055] The top of the reaction bag body 100 is provided with a liquid inlet pipeline 110, the lower end of the liquid inlet pipeline 110 extends into the reaction bag body 100 and extends to the bottom of the reaction bag body 100. The pipe section of the liquid inlet pipeline 110 is fixed to the side wall of the reaction bag body 100 through a double-end inner extension pipe card 120, which can ensure the stability of the liquid inlet pipeline 110 and prevent displacement during the cultivation process. The top of the reaction bag body 100 is also provided with a large flow pipeline 130 and other pipelines.

[0056] The side wall of the reaction bag body 100 is also provided with a sampling pipeline assembly 140, a pH electrode joint 150, a dissolved oxygen electrode joint 160 and a temperature control pipeline assembly 170, which can monitor and control various parameters of the cultivation environment in real time to ensure the best conditions for cell culture. The side wall of the reaction bag body 100 is also provided with at least two corrugated pipe pipeline assembly interfaces 180 for connecting external equipment or adding other substances required for cultivation.

[0057] The cell reaction bag can produce bubbles of different sizes through two groups of holes of different sizes in the aeration device, which can improve the gas dissolution efficiency. The design of the stirring device can fully mix the culture solution and enhance the material transfer. The application of disposable plasma generator can pass the active substances in plasma state and the generated microbubbles into the culture medium together, so as to promote the high expression of cells and stabilize the cell viability. The overall structure is reasonable, and each component is detachably connected, which is convenient for installation and maintenance, and is suitable for large-scale cell culture production.

[0058] Example 2 See Figure 6The embodiment provides a cell reaction bag with a double-air-path ventilation device, and the difference from the embodiment 1 is that a disposable plasma generator 400 is installed on the first air inlet pipeline 270 and / or the second air inlet pipeline 280.

[0059] Reference Figures 7-9 The disposable plasma generator 400 comprises a high-voltage electrode copper pipe 420, a ground electrode copper rod 430, an electrode fixing sheet 440 and a shell 410. Two electrode fixing sheets 440 are arranged at two ends of the high-voltage electrode copper pipe 420 respectively, two ends of the ground electrode copper rod 430 are fixed at the centers of the electrode fixing sheets 440 respectively, and a through hole is further arranged on the electrode fixing sheet 440. The shell 410 is arranged outside the high-voltage electrode copper pipe 420, electrode insertion holes 450 are arranged at two ends of the shell 410 respectively, and the electrode insertion holes 450 are electrically connected with the two ends of the ground electrode copper rod 430 through wires respectively.

[0060] Through the external controller, the electrode is inserted into the electrode insertion hole 450 of the disposable plasma generator 400, the air passing through the hole can be excited into plasma, the active substance in the plasma state and the generated microbubbles are introduced into the culture medium together, and the functions of promoting cell high expression and stabilizing cell activity are realized.

[0061] The distance between the outlet of the disposable plasma generator 400 and the liquid outlet connector 260 is not more than 200 mm, and the distance design can ensure that the plasma does not inactivate before entering the reaction bag, and the plasma treatment effect is guaranteed; the plasma directly bubbles into the culture medium with a short transmission distance, and stimulates cell expression.

[0062] The disposable plasma generator adopts an air excitation mode and does not need inert gas; low-temperature plasma is generated, the outlet temperature is lower than 37 DEG C; a disposable sealed structure is adopted, and the environment is not communicated, so that the sterility is guaranteed; a high air flow rate can be realized, reaches 12.5 L / min; a high plasma density can be generated, reaches 10 5 / cm 3 .

[0063] In summary, the ventilation device of the application forms double-air-path ventilation by arranging two groups of holes with different sizes and corresponding channels, can introduce different gases respectively, and can generate large bubbles outside the ventilation disc and microbubbles inside the ventilation disc; not only improves the gas dissolution efficiency, guarantees the uniform distribution of the gas, and is beneficial to the uniform growth of cells; but also can accurately control the different needs of oxygen, carbon dioxide and other gases in the cell culture process, and provides a more suitable gas environment for cell growth. Therefore, the application effectively overcomes the various shortcomings in the prior art and has high industrial utilization value.

[0064] Wherein, the terms such as "upper", "lower", "left", "right", "front", "back", "middle" and "one" cited in the specification are only for the convenience of clear description, and are not used to limit the scope of the application, and the change or adjustment of the relative relationship is also considered as the scope of the application without substantial change of the technical content.

[0065] The above embodiments only exemplarily illustrate the principles and effects of the application, and are not used to limit the application. All equivalent modifications or changes made by those skilled in the art without departing from the spirit and technical thought disclosed by the application should be covered by the claims of the application.

Claims

1. A cell reaction bag with a dual-channel ventilation device, characterized in that, include: The reaction bag body; A ventilation device, the ventilation device including a ventilation disc, the ventilation disc being disposed at the bottom of the internal space of the reaction bag body; The vent plate has two sets of holes, namely a first set of holes and a second set of holes. The vent plate has a first channel and a second channel inside, which are connected to the first set of holes and the second set of holes, respectively. The bottom of the vent plate has a first air inlet and a second air inlet, which are connected to the first channel and the second channel, respectively. The first air inlet and the second air inlet are connected to a first connecting pipe and a second connecting pipe, respectively. The first connecting pipe and the second connecting pipe are connected to an external first air inlet pipe and a second air inlet pipe, respectively. The stirring device includes a stirring shaft and a paddle. The upper end of the stirring shaft is connected to an external drive mechanism through a sealed bearing, and the lower end of the stirring shaft extends into the reaction bag body and rotates with the venting disc.

2. The cell reaction bag with a dual-channel ventilation device according to claim 1, characterized in that, The first group of holes is located in the center of the vent plate, and the second group of holes surrounds the first group of holes; the diameter of the holes in the first group of holes is smaller than the diameter of the holes in the second group of holes.

3. The cell reaction bag with a dual-channel ventilation device according to claim 1, characterized in that, The ventilation disc is circular, and the first channel and / or the second channel consists of multiple concentrically arranged annular grooves, with slots for communication between adjacent annular grooves.

4. The cell reaction bag with a dual-channel ventilation device according to claim 3, characterized in that, The holes in the first hole group and / or the second hole group are distributed in a concentric circle.

5. The cell reaction bag with a dual-channel ventilation device according to any one of claims 1 to 4, characterized in that, A disposable plasma generator is installed on the first air intake pipe and / or the second air intake pipe.

6. The cell reaction bag with a dual-channel ventilation device according to claim 5, characterized in that, The disposable plasma generator includes a high-voltage electrode copper tube, a ground electrode copper rod, electrode fixing plates, and a housing. Two electrode fixing plates are respectively disposed at both ends of the high-voltage electrode copper tube, and both ends of the ground electrode copper rod are respectively fixed to the center of the electrode fixing plates. The electrode fixing plates are also provided with through holes. The housing covers the high-voltage electrode copper tube, and electrode insertion holes are respectively provided at both ends of the housing. The electrode insertion holes are electrically connected to both ends of the ground electrode copper rod.

7. The cell reaction bag with a dual-channel ventilation device according to claim 5, characterized in that, The distance between the outlet of the disposable plasma generator and the liquid outlet connector shall not exceed 200 mm.

8. The cell reaction bag with a dual-channel ventilation device according to claim 1, characterized in that, A fixed joint is provided at the center of the venting disc, and the lower end of the stirring shaft is rotatably engaged with the fixed joint through a bearing assembly.

9. The cell reaction bag with a dual-channel ventilation device according to claim 8, characterized in that, The bottom of the reaction bag body is provided with a liquid outlet connector. The first connecting pipe and the second connecting pipe are respectively connected to the first air inlet pipe and the second air inlet pipe outside through the liquid outlet connector. The liquid outlet connector is provided with a liquid outlet and is connected to the external liquid outlet pipe. The fixed connector and the liquid outlet connector are connected by columns that are spaced apart, and the columns form a liquid outlet channel that connects to the liquid outlet.

10. The cell reaction bag with a dual-channel ventilation device according to claim 1, characterized in that, The top of the reaction bag body is provided with several liquid inlet pipes, the lower end of which extends into the reaction bag body and extends to the bottom of the reaction bag body; the middle section of the liquid inlet pipe is fixed to the side wall of the reaction bag body.

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