Nutritional composition for improving bone health and preparation method thereof

Abstract

The invention discloses a nutritional composition for improving bone health and a preparation method thereof. The composition is prepared from the following raw materials in parts by mass: 16 to 23 parts by mass of calcium citrate, 5 to 10 parts by mass of L-calcium aspartate, 20 to 32.5 parts by mass of marine collagen peptide, 3 to 5 parts by mass of casein phosphopeptides, 3 to 7 parts by mass of vitamin D3, 3 to 6 parts by mass of vitamin K2 and 4 to 8 parts by mass of radix pseudostellariae cyclic peptide B, the traditional Chinese medicine composition is prepared from, by mass, 5-10 parts of coix seed powder, 15-20 parts of black garlic polysaccharide, 10-18 parts of balsam pear saponin, 20-30 parts of eucommia seed oil and 10-15 parts of chondroitin sulfate. The obtained nutritional composition can effectively promote calcium absorption so as to improve the bone calcium content and bone density, prevent and treat osteoporosis and improve bone health.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of bone nutrition, and more particularly to a nutritional composition for improving bone health and a preparation method thereof. BACKGROUND

[0002] Bone health refers to the health of the entire skeletal system, including bones, cartilage, joints, tendons, ligaments, etc. The most common bone health problems are osteoporosis and osteoarthritis. Simple calcium supplementation has poor effect and cannot guarantee the flexibility, flexibility, shock resistance, tensile strength and overall coordination of the skeletal system. In most cases, the concentration of calcium in the blood is increased, but it is difficult to prevent the loss of calcium in the skeleton, but it is difficult to promote strong and healthy bone growth. Therefore, how to convert absorbed calcium into calcium in the skeleton to improve bone health is a problem that needs to be solved at present. SUMMARY

[0003] The present application aims to overcome the shortcomings of the prior art and provide a nutritional composition for improving bone health and a preparation method thereof. The obtained nutritional composition can effectively promote calcium absorption, increase bone calcium content and bone density, prevent and treat osteoporosis, and improve bone health.

[0004] In order to achieve the above-mentioned purpose, the present application adopts the following technical scheme:

[0005] A nutritional composition for improving bone health, which is made of the following raw materials in mass parts: calcium citrate 16-23 parts by mass, L-calcium aspartate 5-10 parts by mass, marine collagen peptide 20-32.5 parts by mass, casein phosphopeptide 3-5 parts by mass, vitamin D3 3-7 parts by mass, vitamin K2 3-6 parts by mass, radix notoginseng cyclic peptide B 4-8 parts by mass, coix seed powder 5-10 parts by mass, black garlic polysaccharide 15-20 parts by mass, bitter gourd saponin 10-18 parts by mass, eucommia seed oil 20-30 parts by mass, and chondroitin sulfate 10-15 parts by mass.

[0006] The present application further provides that the composition is made of the following raw materials in mass parts: calcium citrate 20 parts by mass, L-calcium aspartate 8.5 parts by mass, marine collagen peptide 22 parts by mass, casein phosphopeptide 3.7 parts by mass, vitamin D3 5 parts by mass, vitamin K2 4 parts by mass, radix notoginseng cyclic peptide B 6.1 parts by mass, coix seed powder 8 parts by mass, black garlic polysaccharide 18.6 parts by mass, bitter gourd saponin 15 parts by mass, eucommia seed oil 25 parts by mass, and chondroitin sulfate 12 parts by mass.

[0007] A preparation method of a nutritional composition for improving bone health, comprising the following steps:

[0008] S1, chondroitin sulfate, marine collagen peptide, casein phosphopeptide, calcium L-aspartate, momordica charantia saponin, black garlic polysaccharide, water are mixed to form an aqueous phase mixture;

[0009] S2, vitamin D3, vitamin K2 are added to eucommia seed oil, and stirred and mixed to form an oil phase mixture;

[0010] S3, the oil phase mixture is added to the aqueous phase mixture, and homogenized to obtain a water-oil mixture;

[0011] S4, the yiyiren powder and calcium citrate are added to the water-oil mixture in a proportion, and then the ginseng cyclic peptide B is added and uniformly mixed; the obtained mixture is spray dried and sterilized to obtain a bone health improvement nutritional composition.

[0012] The application is further provided that the aqueous phase mixture in step S1 contains 60-70wt% of water.

[0013] The application is further provided that step S1 is specifically: chondroitin sulfate is dissolved in water to form a chondroitin sulfate solution, marine collagen peptide and casein phosphopeptide are added and uniformly mixed to obtain system A; calcium L-aspartate is dissolved in water, and then momordica charantia saponin and black garlic polysaccharide are added and uniformly mixed to obtain system B; system A and system B are uniformly mixed to form an aqueous phase mixture.

[0014] The application is further provided that system A is prepared at room temperature.

[0015] The application is further provided that calcium L-aspartate is dissolved in water at 30-50℃.

[0016] The application is further provided that, during spray drying, the inlet air temperature is 180-200℃, and the outlet air temperature is 80-90℃.

[0017] In summary, the application has the following beneficial effects:

[0018] The reasonable compounding of calcium citrate, calcium L-aspartate, marine collagen peptide, casein phosphopeptide, vitamin D3, vitamin K2, ginseng cyclic peptide B, yiyiren powder, momordica charantia saponin, black garlic polysaccharide, eucommia seed oil and chondroitin sulfate obtains a nutritional composition, which can effectively promote calcium absorption, increase bone calcium content and bone density, prevent and treat osteoporosis, and improve bone health. DETAILED DESCRIPTION

[0019] The technical solutions of the present application will be clearly and completely described below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0020] The bone health improving nutritional composition is prepared from the following raw materials by mass fraction: the composition is prepared from the following raw materials by mass fraction: 16-23 parts by mass of calcium citrate, 5-10 parts by mass of calcium L-aspartate, 20-32.5 parts by mass of marine collagen peptide, 3-5 parts by mass of casein phosphopeptide, 3-7 parts by mass of vitamin D3, 3-6 parts by mass of vitamin K2, 4-8 parts by mass of radix notoginseng cyclic peptide B, 5-10 parts by mass of coix seed powder, 15-20 parts by mass of black garlic polysaccharide, 10-18 parts by mass of momordica charantia saponin, 20-30 parts by mass of eucommia seed oil, and 10-15 parts by mass of chondroitin sulfate. The preparation method is as follows:

[0021] S1, at room temperature, chondroitin sulfate is dissolved in water to form a chondroitin sulfate solution, marine collagen peptide and casein phosphopeptide are added and uniformly mixed to obtain system A; calcium L-aspartate is dissolved in water, and momordica charantia saponin and black garlic polysaccharide are then added and uniformly mixed to obtain system B; then system A and system B are uniformly mixed to prepare a water phase mixture (the water phase mixture contains 60-70 wt% of water);

[0022] S2, vitamin D3 and vitamin K2 are added to eucommia seed oil, and stirred and mixed to form an oil phase mixture;

[0023] S3, the oil phase mixture is added to the water phase mixture, and uniformly mixed to obtain a water-oil mixture;

[0024] S4, coix seed powder and calcium citrate are added to the water-oil mixture in a proportion, and radix notoginseng cyclic peptide B is then added and uniformly mixed; the obtained mixture is spray dried (the inlet temperature is 180-200℃, and the outlet temperature is 80-90℃ during spray drying), sterilized, and a bone health improving nutritional composition is obtained.

[0025] Example 1

[0026] S1, at room temperature, 12 parts by mass of chondroitin sulfate is dissolved in water to form a chondroitin sulfate solution, 22 parts by mass of marine collagen peptide and 3.7 parts by mass of casein phosphopeptide are added and uniformly mixed to obtain system A; 8.5 parts by mass of calcium L-aspartate is dissolved in water at 40℃, and 15 parts by mass of momordica charantia saponin and 18.6 parts by mass of black garlic polysaccharide are then added and uniformly mixed to obtain system B; then system A and system B are uniformly mixed to prepare a water phase mixture (the water phase mixture contains 65.3 wt% of water);

[0027] S2, 5 parts by mass of vitamin D3 and 4 parts by mass of vitamin K2 were added to 25 parts by mass of the eucommia seed oil, and stirred and mixed to form an oil phase mixture;

[0028] S3, the oil phase mixture was added to the water phase mixture, and homogenously mixed to obtain a water-oil mixture;

[0029] S4, 8 parts by mass of coix seed powder and 20 parts by mass of calcium citrate were added to the water-oil mixture, and 6.1 parts by mass of notoginseng cyclic peptide B was further added and mixed uniformly; the obtained mixture was spray dried (the inlet temperature was 180°C and the outlet temperature was 85°C during spray drying), sterilized, and a bone health improving nutritional composition was obtained.

[0030] Comparative Example 1

[0031] The same mass of calcium citrate, L-calcium aspartate, marine collagen peptide, casein phosphopeptide, vitamin D3, vitamin K2, notoginseng cyclic peptide B, coix seed powder, momordica charantia saponin, black garlic polysaccharide, eucommia seed oil, chondroitin sulfate, and water were added at one time, mixed uniformly, and then spray dried (the inlet temperature was 180°C and the outlet temperature was 85°C during spray drying), sterilized, and a bone health improving nutritional composition was obtained.

[0032] Comparative Example 2

[0033] The bone health improving nutritional composition was prepared according to the method of Example 1, but the same mass of notoginseng cyclic peptide B was replaced by calcium citrate.

[0034] Comparative Example 3

[0035] The bone health improving nutritional composition was prepared according to the method of Example 1, but the same mass of coix seed powder was replaced by calcium citrate.

[0036] Comparative Example 4

[0037] The bone health improving nutritional composition was prepared according to the method of Example 1, but the same mass of momordica charantia saponin was replaced by calcium citrate.

[0038] Comparative Example 5

[0039] The bone health improving nutritional composition was prepared according to the method of Example 1, but the same mass of black garlic polysaccharide was replaced by calcium citrate.

[0040] The bone health improving nutritional composition prepared in Example 1 and Comparative Examples 1-5 was tested for bone mineral density and bone calcium content.

[0041] Subject: 70 SPF female Wistar rats, each weighing 250±10 grams.

[0042] Experimental method: The rats were raised in an environment of room temperature 25℃, relative humidity 50%, the rats were given light for 12 hours per day, and deionized water was drunk, and low-calcium feed (calcium content was 0.15%, purchased from Xiaoshuoyoutai (Beijing) Biotechnology Co., Ltd.) was fed for 1 week. Then the rats were randomly divided into 7 groups, i.e. a low-calcium group, an Example 1 composition group, a Comparative Example 1 composition group to a Comparative Example 5 composition group, 10 rats in each group. Once a day for 12 weeks (i.e. the low-calcium group of rats was given deionized water by gavage, 10 mL / kg·bw each time; the Example 1 composition group, the Comparative Example 1 composition group to the Comparative Example 5 composition group were given the corresponding Example 1 composition, the Comparative Example 1 composition group to the Comparative Example 5 composition group, each time the dosage was 375 mg / kg·bw); during the test, the rats in each group were fed with low-calcium feed (30 g per day), and water was drunk freely. After the end of the 12th week test, the rats were weighed, and then sacrificed, and the bilateral femurs were stripped and placed in an oven at 105℃ to dry to constant weight, and then cooled to room temperature and weighed; after weighing, they were placed in a clean and dry triangular bottle treated with hydrochloric acid, 15 mL of nitric acid: perchloric acid (volume ratio 4:1) was added for digestion, and deionized water was added to 25 mL, and atomic absorption spectrophotometry was used to determine the calcium content in the bone; the bone mineral density (BMD) at the midpoint and distal end of the femur was measured by a bone mineral meter. The average bone density and average bone calcium content test results are shown in Table 1.

[0043] Table 1

[0044]

[0045] The nutritional compositions prepared in Example 1, Comparative Example 1 to Comparative Example 5 were tested for their arthritis treatment effect, and the test method was as follows:

[0046] (1) Test object: SPF female Wistar rats, each rat weighing 250±10 grams.

[0047] (2) Experimental grouping and dosage design: the rats were subjected to FCA modeling, and on the 13th day of modeling, 60 rats with obvious secondary lesions were selected for grouping, 10 rats in each group, and randomly divided into 6 groups: an Example 1 composition group, a Comparative Example 1 composition group to a Comparative Example 5 composition group.

[0048] The modeling method was as follows: the same batch of bacillus calmette guerin (BCG) was inactivated at 80℃ for 1 hour, and a 10 g / L emulsion was prepared with high-pressure sterilized liquid paraffin, and then ground and mixed evenly to obtain Freund's complete adjuvant (FCA), and then 0.1 ml of FCA was injected intradermally into the left hind toe of the rat to cause inflammation.

[0049] (3) The time and method of administration: after two weeks of immunization, the gavage administration was started, once a day, and the administration was continuously performed for 30 days. When gavaging, the composition groups of Example 1, the composition groups of Comparative Example 1 to Comparative Example 5 were gavaged with the composition of Example 1, the composition of Comparative Example 1 to Comparative Example 5, respectively, and the gavaged composition dose was 50 mg / kg / day, and the gavaged drug volume was 25 mL.

[0050] (4) The observation of the joint swelling degree of rats in different test groups: the occurrence and severity of the multiple arthritis of rats in each group were recorded by using the joint score method. Each paw was scored according to the following standard: 0 = normal; 1 = slight swelling and redness of the ankle joint; 2 = slight swelling and redness of the ankle joint to the metatarsal joint or the palm joint; 3 = moderate swelling and redness of the ankle joint to the metatarsal joint or the palm joint; 4 = severe swelling and redness of the ankle joint to the metatarsal joint.

[0051] After 30 days, the average joint swelling degree of rats in the composition group of Example 1, the composition group of Comparative Example 1 to the composition group of Comparative Example 5 was 0.5, 1.7, 2.2, 2.0, 1.9, 2.1, respectively (the average joint swelling degree of rats in each group before gavage was 4).

[0052] From the above results, it can be seen that the nutritional composition of the present application can prevent and treat osteoporosis, improve arthritis, and promote bone health.

[0053] The above only describes the preferred embodiments of the present application, and the protection scope of the present application is not limited to the above-described embodiments only, and any technical solution falling within the idea of the present application belongs to the protection scope of the present application. It should be noted that, for ordinary skilled persons in the art, some improvements and decorations without departing from the principle of the present application should also be considered as the protection scope of the present application.

Claims

1. A nutritional composition for improving bone health, characterized in that, The composition is made of the following raw materials by mass fraction: calcium citrate 16-23 parts by mass, calcium L-aspartate 5-10 parts by mass, marine collagen peptide 20-32.5 parts by mass, casein phosphopeptide 3-5 parts by mass, vitamin D3 3-7 parts by mass, vitamin K2 3-6 parts by mass, radix notoginseng cyclic peptide B 4-8 parts by mass, coix seed powder 5-10 parts by mass, black garlic polysaccharide 15-20 parts by mass, momordica charantia saponin 10-18 parts by mass, eucommia seed oil 20-30 parts by mass, and chondroitin sulfate 10-15 parts by mass.

2. The nutritional composition for improving bone health according to claim 1, wherein The composition is made of the following raw materials by mass fraction: calcium citrate 20 parts by mass, calcium L-aspartate 8.5 parts by mass, marine collagen peptide 22 parts by mass, casein phosphopeptide 3.7 parts by mass, vitamin D3 5 parts by mass, vitamin K2 4 parts by mass, radix notoginseng cyclic peptide B 6.1 parts by mass, coix seed powder 8 parts by mass, black garlic polysaccharide 18.6 parts by mass, momordica charantia saponin 15 parts by mass, eucommia seed oil 25 parts by mass, and chondroitin sulfate 12 parts by mass.

3. A method of preparing the nutritional composition for improving bone health according to any one of claims 1-2, characterized in that, The method comprises the following steps: S1, mixing chondroitin sulfate, marine collagen peptide, casein phosphopeptide, calcium L-aspartate, momordica charantia saponin, black garlic polysaccharide and water to form a water phase mixture; S2, adding vitamin D3 and vitamin K2 to eucommia seed oil, stirring and mixing to form an oil phase mixture; S3, adding the oil phase mixture to the water phase mixture, homogenizing and mixing to obtain a water-oil mixture; S4, adding coix seed powder and calcium citrate to the water-oil mixture in a certain proportion, and then adding radix notoginseng cyclic peptide B and mixing uniformly; the obtained mixture is spray dried and sterilized to obtain the nutritional composition for improving bone health.

4. The method for preparing a nutritional composition for improving bone health according to claim 3, characterized in that, The water phase mixture of step S1 contains 60-70 wt% of water.

5. The method for preparing a nutritional composition for improving bone health according to claim 3, characterized in that, Step S1 is specifically: dissolving chondroitin sulfate in water to form a chondroitin sulfate solution, adding marine collagen peptide and casein phosphopeptide and mixing uniformly to obtain system A; dissolving calcium L-aspartate in water, then adding momordica charantia saponin and black garlic polysaccharide and mixing uniformly to obtain system B; mixing system A and system B uniformly to form a water phase mixture.

6. The method for preparing a nutritional composition for improving bone health according to claim 5, characterized in that, System A is prepared at room temperature.

7. The method for preparing a nutritional composition for improving bone health according to claim 5, characterized in that, Calcium L-aspartate is dissolved in water at 30-50℃.

8. The method for preparing a nutritional composition for improving bone health according to claim 3, characterized in that, During spray drying, the temperature of the air inlet is 180-200℃, and the temperature of the air outlet is 80-90℃.

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