A composition for assisting improvement of memory and use thereof

Abstract

The application discloses a kind of compositions for assisting to improve memory and application thereof, and specifically relates to health product technical field.The composition includes broccoli leaf polypeptide, white mushroom polypeptide, gastrodin protein peptide and N-acetylneuraminic acid, and the mass ratio is 1:(0.5-1.5):(2-3):(0.1-0.3).The application promotes acetylcholine secretion by four components synergistically, reduces acetylcholinesterase content, so that the composition has the efficacy of assisting to improve memory and cognitive ability, and can be applied to health product.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of health products, in particular to a composition for assisting in improving memory and application thereof. BACKGROUND

[0002] In the field of functional products for assisting in improving memory, polypeptide substances are widely studied and applied due to their small molecular weight and high biological activity. However, the compositions in the prior art have the following significant bottlenecks:

[0003] Unclear synergistic mechanism: the existing compositions often use single or simply mixed polypeptide components, lack of scientific compatibility, leading to insufficient synergistic effect between active ingredients, and difficult to fully play the role of assisting in improving memory.

[0004] Poor stability: some polypeptide components are easily affected by environmental factors (such as temperature, pH value), leading to reduced biological activity, affecting the shelf life and efficacy stability of the product.

[0005] Low cost performance: some compositions are limited in large-scale application in the field of health products due to complex components or high raw material cost.

[0006] Therefore, the present application selects specific polypeptide components (broccoli leaf polypeptide, white mushroom polypeptide, and gastrodin protein peptide) and N-acetylneuraminic acid for scientific compounding, constructs a composition with clear synergistic mechanism, and verifies the regulation effect on acetylcholine secretion and acetylcholinesterase content, so as to overcome the defects of the prior art and provide a composition for assisting in improving memory with high efficiency and stability. SUMMARY

[0007] In view of the deficiencies in the prior art, the present application provides a composition for assisting in improving memory and application thereof, which has a significant effect of improving memory.

[0008] In a first aspect, the present application provides a composition for assisting in improving memory, which comprises the following raw materials: broccoli leaf polypeptide, white mushroom polypeptide, gastrodin protein peptide, and N-acetylneuraminic acid, and the mass ratio is 1:0.5-1.5:2-3:0.1-0.3.

[0009] Preferably, the mass ratio of broccoli leaf polypeptide, white mushroom polypeptide, gastrodin protein peptide, and N-acetylneuraminic acid in the composition is 1:0.5-1:2-2.5:0.1-0.2.

[0010] Further preferably, the mass ratio of broccoli leaf polypeptide, white mushroom polypeptide, gastrodin protein peptide, and N-acetylneuraminic acid in the composition is 1:1:2.5:0.2.

[0011] In a second aspect, the present application provides use of the composition for improving memory in the first aspect in the preparation of health products with the effect of improving memory.

[0012] In a third aspect, the present application provides a health product with the effect of improving memory, which comprises 20-30 wt% of the composition for improving memory in the first aspect.

[0013] Preferably, the health product further comprises at least one of a sweetener, a thickening agent, nicotinamide, and milk powder.

[0014] Preferably, the sweetener is at least one of white granulated sugar, brown sugar, black sugar, glucose, fructose, sucralose, acesulfame potassium, chloro sucrose, sorbitol, maltitol, xylitol, mannitol, and erythritol.

[0015] Preferably, the thickening agent is at least one of xanthan gum, tamarind seed polysaccharide gum, resistant dextrin, carrageenan, and gelatin.

[0016] Further preferably, the thickening agent is tamarind seed polysaccharide gum, resistant dextrin, and carrageenan, and the mass ratio is 1:2-4:0.5-1.5.

[0017] Preferably, the milk powder is full-fat milk powder or skimmed milk powder.

[0018] Preferably, the health product with the effect of improving memory comprises the following components: the composition, the sweetener, the thickening agent, nicotinamide, and milk powder, and the preparation method comprises the following steps:

[0019] Mixing the composition, the sweetener, the thickening agent, nicotinamide, and milk powder uniformly, sterilizing by irradiation, and sealing for storage.

[0020] Compared with the prior art, the present application has the following beneficial effects:

[0021] The composition provided by the present application has a significant effect of improving memory. Specifically, the composition of the present application can moderately increase the acetylcholine (ACh) level of a cognitive impairment mouse and reduce the content of acetylcholinesterase, wherein the moderate increase in the acetylcholine level can enhance the communication between neurons, and the reduction in the content of acetylcholinesterase can increase the residence time of acetylcholine in the synaptic cleft, which makes the neural signal transmission more persistent, helps to strengthen the process of memory and learning, and is crucial for the formation of short-term memory and the consolidation of long-term memory. DETAILED DESCRIPTION

[0022] In order to better understand the present application, the present application will be further described below in conjunction with specific examples, wherein the terms used in the composition or test examples are for describing specific embodiments and do not constitute a limitation on the scope of protection of the present application.

[0023] The experimental methods in the following compositions or test examples without specific conditions are generally according to the conventional conditions, or according to the conditions suggested by the manufacturers. Unless otherwise specified, the percentages and parts are calculated by weight.

[0024] Part of the raw materials involved in the present application:

[0025] Broccoli leaf polypeptide: purchased from Lanzhou Waterless Biological Technology Co., Ltd.

[0026] White mushroom polypeptide: purchased from Shaanxi Snort Biological Technology Co., Ltd.

[0027] Gastrodia elata protein peptide: purchased from Fufeng Snort Biological Technology Co., Ltd.

[0028] Acetylneuraminic acid: purchased from Xi'an Haotian Biological Engineering Co., Ltd.

[0029] The total mass fraction of the composition for improving memory in the specific embodiment of the present application is 100 parts, and the raw materials and their mass ratio are as follows:

[0030] Composition 1:

[0031] Consisting of broccoli leaf polypeptide, white mushroom polypeptide, gastrodia elata protein peptide, and N-acetylneuraminic acid with a mass ratio of 1:1:2.5:0.2.

[0032] Composition 2:

[0033] Consisting of broccoli leaf polypeptide, white mushroom polypeptide, gastrodia elata protein peptide, and N-acetylneuraminic acid with a mass ratio of 1:0.5:2:0.1.

[0034] Composition 3:

[0035] Consisting of broccoli leaf polypeptide, white mushroom polypeptide, gastrodia elata protein peptide, and N-acetylneuraminic acid with a mass ratio of 1:1.5:3:0.3.

[0036] Composition ①:

[0037] Consisting of white mushroom polypeptide, gastrodia elata protein peptide, and N-acetylneuraminic acid with a mass ratio of 1:2.5:0.2.

[0038] Composition ②:

[0039] Consisting of broccoli leaf polypeptide, gastrodia elata protein peptide, and N-acetylneuraminic acid with a mass ratio of 1:2.5:0.2.

[0040] Composition ③:

[0041] Consisting of broccoli leaf polypeptide, white mushroom polypeptide, and N-acetylneuraminic acid with a mass ratio of 1:1:0.2.

[0042] Composition 4:

[0043] It is composed of broccoli leaf polypeptide, white mushroom polypeptide and Gastrodia elata protein peptide with a mass ratio of 1:1:2.5.

[0044] Composition 5:

[0045] It is composed of broccoli leaf polypeptide, white mushroom polypeptide, Gastrodia elata protein peptide and N-acetylneuraminic acid with a mass ratio of 1:2:1:0.5.

[0046] The preparation method of the above-mentioned composition for assisting in improving memory comprises the following steps: uniformly mixing the raw materials according to the formula amount, and sealing and storing in the dark.

[0047] The health care product with the effect of assisting in improving memory in the application comprises the following mass percentage components:

[0048] Health care product 1:

[0049] Composition: 25wt%;

[0050] Sweetener: 0.4wt%;

[0051] Thickening agent: 2wt%;

[0052] Nicotinamide: 0.5wt%;

[0053] Milk powder: supplemented to 100wt%;

[0054] The composition is composition 1; the sweetener is sucralose; the thickening agent is composed of tamarind polysaccharide gum, resistant dextrin and carrageenan with a mass ratio of 1:3:1; and the milk powder is full-fat milk powder.

[0055] The preparation method of health care product 1: uniformly mix the composition, sweetener, thickening agent, nicotinamide and milk powder, sterilize by irradiation, and seal and store.

[0056] Health care product 2:

[0057] Composition: 20wt%;

[0058] Sweetener: 0.4wt%;

[0059] Thickening agent: 2wt%;

[0060] Nicotinamide: 0.5wt%;

[0061] Milk powder: supplemented to 100wt%;

[0062] The composition is composition 1; the sweetener is sucralose; the thickening agent is composed of tamarind polysaccharide gum, resistant dextrin and carrageenan with a mass ratio of 1:3:1; and the milk powder is full-fat milk powder.

[0063] The preparation method of health product 2 is the same as that of health product 1.

[0064] Health product 3:

[0065] Composition: 30wt%;

[0066] Sweetener: 0.4wt%;

[0067] Thickening agent: 2wt%;

[0068] Nicotinamide: 0.5wt%;

[0069] Milk powder: supplement to 100wt%;

[0070] The composition is composition 1; the sweetener is sucralose; the thickening agent is composed of tamarind polysaccharide gum, resistant dextrin and carrageenan with a mass ratio of 1:3:1; and the milk powder is full-fat milk powder.

[0071] The preparation method of health product 3 is the same as that of health product 1.

[0072] Health product 4:

[0073] Composition: 25wt%;

[0074] Sweetener: 0.4wt%;

[0075] Thickening agent: 2wt%;

[0076] Nicotinamide: 0.5wt%;

[0077] Milk powder: supplement to 100wt%;

[0078] The composition is composition 2; the sweetener is sucralose; the thickening agent is composed of tamarind polysaccharide gum, resistant dextrin and carrageenan with a mass ratio of 1:3:1; and the milk powder is full-fat milk powder.

[0079] The preparation method of health product 4 is the same as that of health product 1.

[0080] Health product 5:

[0081] Composition: 25wt%;

[0082] Sweetener: 10wt%;

[0083] Thickening agent: 2wt%;

[0084] Nicotinamide: 0.5wt%;

[0085] Full-fat milk powder: supplement to 100wt%;

[0086] The composition is composition 3; the sweetener is white granulated sugar; the thickening agent is composed of tamarind polysaccharide gum, resistant dextrin and carrageenan in a mass ratio of 1:3:1;

[0087] The preparation method of health product 5 is the same as that of health product 1.

[0088] Blank health product:

[0089] Different from health product 1, whole milk powder is used to replace the composition in an equal amount, and the remaining raw materials and mass percentages are the same as those of health product 1.

[0090] The preparation method of the blank health product is to mix the sweetener, the thickening agent, the nicotinamide and the milk powder uniformly, irradiate and sterilize, and seal and store.

[0091] Test example 1: behavioral evaluation

[0092] 1.1. Test sample solution and its preparation method:

[0093] The test sample is composition 1-3 and composition ①-⑤.

[0094] The preparation method of the test sample solution is to prepare the test sample solution to 1 mg / mL using normal saline, and to prepare it immediately before use.

[0095] 1.2. Animal treatment and grouping

[0096] 7-week-old male C57BL / 6 mice (SPF level) are raised in an environment with a temperature of 22±2℃, a humidity of 55±2%, and a 12h:12h light-dark cycle, and are allowed to eat and drink freely. After one week of adaptation, the mice are randomly divided into a control group, a model group, and a test group, a total of 10 groups, 20 mice in each group; 20 mice in each group are randomly divided into A and B groups, which participate in two behavioral evaluation tests, respectively.

[0097] The control group is intraperitoneally injected with normal saline and orally administered with normal saline; the model group is intraperitoneally injected with D-galactose at a dose of 800 mg / Kg of body weight per day and orally administered with normal saline; the test group is intraperitoneally injected with D-galactose at a dose of 800 mg / Kg of body weight per day and orally administered with the test sample solution every day. The intraperitoneal injection amount and the oral administration amount are 0.2 mL per mouse.

[0098] After 12 weeks, a series of behavioral tests are performed on each group, and after the behavioral tests are completed, the mice are sacrificed by decapitation to collect the hippocampus, which is immediately frozen in liquid nitrogen and stored at -80℃.

[0099] 1.3. Nest building test behavioral evaluation

[0100] The mice were placed in a clean cage alone, and 32 pieces of square paper (5 cm x 5 cm) were evenly distributed in the cage, allowing the mice to freely move in the cage for 12 hours. The nest scores were statistically analyzed according to the following criteria (a total of 4 points), and the statistical analysis criteria were as follows:

[0101] No nest: 1 point; no obvious nest, nest paper more than 1 / 3 of the cage space: 2 points; obvious nest building, nest paper accounting for 1 / 4-1 / 3 of the cage space: 3 points; bowl nest or nest paper less than 1 / 4 of the cage space: 4 points.

[0102] The average value (retaining 1 decimal place) of the scores of the mice in each group was shown in Table 1 as follows.

[0103] 1.4. Avoiding dark test behavior evaluation

[0104] The dark avoidance experiment is also called passive avoidance experiment. A test box is designed and manufactured according to the habit of mice avoiding light and seeking darkness. The test box has a small hole door connecting one half of the dark room and one half of the light room. The bottom of the dark room is paved with a copper grid connected to electricity, and the animal will be shocked when entering the dark room.

[0105] The dark avoidance experiment lasted for 3 days. The training experiment started at 9:00 am on the first day, and then the test was conducted at 15:00 on the same day, 9:00 am on the second day, and 9:00 am on the third day, respectively. The number of foot shocks of the mice within 5 minutes was recorded. The average value (retaining 1 decimal place) of the shock times of the mice in each group was shown in Table 1 as follows.

[0106] Table 1. Results of behavior evaluation

[0107] group nesting score shock times control group 3.1 8.3 model group 1.2 24.4 composition 1 2.9 9.7 composition 2 2.8 9.9 composition 3 2.7 10.4 composition ① 2.2 16.3 composition ② 1.8 17.7 composition ③ 1.9 17.9 composition ④ 1.6 19.8 composition ⑤ 2.5 14.1

[0108] Result analysis: In the nest building behavior test, the nest score of the model group was significantly lower than that of the test sample group and the control group, and the nest scores of the compositions 1-3 in the test sample group had no significant difference with the control group, indicating that the use of the composition of the present application can effectively improve the cognitive impairment of mice; by comparing the nest scores of composition 1, composition ①-④ and the model group, although composition 1 and composition ①-④ have certain effect on improving the cognitive impairment of mice, but compared with composition 1, the improvement effect of composition ①-④ is obviously poorer, which indicates that the use of broccoli leaf polypeptide, white mushroom polypeptide, gastrodia elata protein peptide and N-acetylneuraminic acid in the present application has a synergistic effect on improving the cognitive impairment of mice; by comparing the scores of composition 1 and composition ⑤, it can be seen that the composition prepared by using the broccoli leaf polypeptide, white mushroom polypeptide, gastrodia elata protein peptide and N-acetylneuraminic acid in the present application has more obvious effect on improving the cognitive impairment of mice.

[0109] The dark avoidance test of mice has high sensitivity to memory process, especially memory reproduction ability. The foot shock times of the test sample group are significantly lower than those of the model group, indicating that the composition can effectively improve the memory reproduction ability of mice. By comparing the shock times of the composition 1 group, the composition 1-4 group and the model group, it can be seen that although the composition 1 and the composition 1-4 have certain effects on improving the memory reproduction ability of mice, the improvement effect of the composition 1 is more obvious compared with the composition 1-4, which indicates that the broccoli leaf polypeptide, the white beech mushroom polypeptide, the gastrodin protein peptide and N-acetylneuraminic acid used in the present application have a synergistic effect on improving the memory reproduction ability of mice. By comparing the shock times of the composition 1 and the composition 5, it can be seen that the composition prepared by using the broccoli leaf polypeptide, the white beech mushroom polypeptide, the gastrodin protein peptide and N-acetylneuraminic acid in the quality ratio of the present application has a more obvious effect on improving the memory reproduction ability of mice.

[0110] Test Example 2: Physicochemical test

[0111] Acetylcholine (ACh) is a neurotransmitter that is essential for brain and nervous system function, including memory and cognitive function, mood control, and muscle control.

[0112] The hippocampus of the above test mice was taken in equal quality into a centrifugal tube, 0.5 mL of pre-cooled PBS buffer was added, and a tissue grinder (frequency: 500 Hz, time: 300 s, temperature: -30℃) was used for grinding, centrifugation (speed: 8000 rmp, time: 10 min, temperature: 4℃), and the supernatant was taken. The contents of acetylcholine (ACh) and acetylcholinesterase (AchE) were detected by ELISA kit, and the results were expressed as mean ± standard deviation, as shown in Table 2 below.

[0113] Table 2 Effect of composition on the contents of acetylcholine and acetylcholinesterase in the hippocampus of mice

[0114] group ACh (μg / mL) AchE (μg / mL) control group 427.01±10.60* 159.86±11.75* model group 277.21±8.18* 274.18±10.53* composition 1 349.07±15.05 179.69±13.57 composition 2 337.47±11.95 180.47±12.04 composition 3 332.41±10.46 185.79±9.51 composition ① 297.03±10.82* 236.26±9.23* composition ② 288.05±10.78* 243.33±6.88* composition ③ 291.25±11.07* 238.56±7.29* composition ④ 280.73±8.52* 246.59±6.12* composition ⑤ 309.04±7.23* 222.03±6.25*

[0115] Note: “*” indicates that p < 0.05 compared with composition 1

[0116] By comparing the results of composition 1 and composition 1-5 in Table 2, it can be seen that the composition provided by the present application can significantly increase the content of acetylcholine in the hippocampus of mice and reduce the content of acetylcholinesterase, which is consistent with the results of the behavior test.

[0117] Test Example 3: Sensory properties

[0118] Test sample: health products 1-5, blank health product.

[0119] Sample preparation method: Take the health product, mix it with water, stir well, and cool it to room temperature. The mass ratio of health product to water is 1:3. The water is drinking water with a temperature of 90-100℃.

[0120] Sensory evaluators with professional training were invited to conduct sensory evaluations of the color, appearance, taste, dispersibility, texture, and mouthfeel of the health products after brewing. The evaluation results are shown in Table 3.

[0121] Table 3. Sensory characteristics evaluation of health supplements before and after brewing.

[0122]

[0123] As shown in Table 3, the health product prepared by this invention has a uniform texture, no lumps, no off-odors, good dispersibility after being brewed with water, uniform brewing, and a rich milky aroma.

[0124] The embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and these variations still fall within the protection scope of the present invention.

Claims

1. A composition for assisting in improving memory, characterized in that, It is composed of broccoli leaf polypeptide, white lingzhi polypeptide, gastrodia protein peptide and N-acetylneuraminic acid, with a mass ratio of 1:0.5-1.5:2-3:0.1-0.

3.

2. The composition according to claim 1, characterized in that, The mass ratio of broccoli leaf peptide, white lingzhi peptide, gastrodia protein peptide, and N-acetylneuraminic acid in the composition is 1:0.5-1:2-2.5:0.1-0.

2.

3. The composition according to claim 2, characterized in that, The mass ratio of broccoli leaf peptide, white lingzhi peptide, gastrodia protein peptide, and N-acetylneuraminic acid in the composition is 1:1:2.5:0.

2.

4. The use of the composition according to any one of claims 1-3 in the preparation of a health product having the effect of assisting in improving memory.

5. A health product with the effect of assisting in improving memory, characterized in that, A composition comprising 20-30 wt% of any one of claims 1-3 for improving memory.

6. The health product according to claim 5, characterized in that, The health supplement also includes at least one of sweeteners, thickeners, nicotinamide, and milk powder.

7. The health product according to claim 6, characterized in that, The milk powder is whole milk powder or skim milk powder.

8. The health product according to claim 6, characterized in that, The sweetener is at least one of the following: white sugar, brown sugar, black sugar, glucose, fructose, cyclamate, acesulfame potassium, sucralose, sorbitol, maltitol, xylitol, mannitol, and erythritol.

9. The health product according to claim 6, characterized in that, The thickener is at least one of xanthan gum, tamarind gum, resistant dextrin, carrageenan, and gelatin.

10. The health product according to claim 9, characterized in that, The thickener is tamarind polysaccharide gum, resistant dextrin, and carrageenan, with a mass ratio of 1:2-4:0.5-1.5.

Images