Method for synthesizing calcifediol by using photocatalysis device

CN121087136APending Publication Date: 2025-12-09TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Application Number
CN202511160822.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-19
Publication Date
2025-12-09

AI Technical Summary

Technical Problem

The existing chemical synthesis route for calcidiol is long, has poor selectivity, and requires harsh reaction conditions. In addition, the enzyme stability in the biosynthesis method is poor and heterologous expression is difficult, resulting in high production costs and limited raw materials, making it difficult to meet the needs of large-scale applications.

Method used

A photocatalytic device was used to catalyze the formation of 25-hydroxy-7-dehydrocholesterol from 7-dehydrocholesterol using AaeUPO nonspecific peroxidase, and then to generate precalcidiol through a light-induced ring-opening reaction. Finally, calcidiol crystals were obtained through thermal isomerization and low-temperature crystallization.

Benefits of technology

This method improves the synthesis efficiency of calcidiol, reduces impurities, simplifies the operation steps, and reduces the amount of organic solvent used, thus achieving efficient calcidiol production and possessing industrial application value.

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Abstract

The invention belongs to the technical field of biological catalysis, and particularly discloses a method for synthesizing calcifediol by using a photocatalytic device. According to the method, 7-dehydrocholesterol is used as a substrate, and 25-hydroxy-7-dehydrocholesterol is obtained through the steps of non-specific oxide enzyme catalysis, extraction, reduced pressure distillation and the like. Dissolving the 25-hydroxy-7-dehydrocholesterol in a polar solvent, a non-polar solvent or a mixed solvent system of the polar solvent and the non-polar solvent, adding an anti-oxidation reagent, and carrying out an illumination isomerization reaction; and under the protection of inert gas, carrying out thermal isomerization reaction on the reaction liquid after photochemical treatment, then carrying out low-temperature crystallization to obtain part of calcifediol crystals, collecting the filtrate after crystallization, and carrying out thermal isomerization reaction and low-temperature crystallization for multiple times to obtain the residual calcifediol crystals. The method has the advantages of simple operation steps, small usage amount of the organic solvent, high synthesis efficiency and cost saving, and is beneficial to large-scale industrial production of calcifediol.
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