Composition for calendula callus culture and application thereof

By optimizing the calendula callus culture system and using specific hormone combinations and culture media, the problems of long extraction cycles and poor stability of calendula active ingredients have been solved, achieving efficient and stable calendula callus culture and extraction, which is suitable for multifunctional skin products.

CN121100799APending Publication Date: 2025-12-12GUANGDONG COOPERATE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202511253771.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2025-07-03
Filing Date
2025-09-03
Publication Date
2025-12-12

AI Technical Summary

Technical Problem

The lack of a mature suspension cell culture system for calendula callus in current technology leads to a long extraction cycle for active ingredients from calendula, and the content is greatly affected by environmental fluctuations. In addition, there is little research on calendula leaf-induced callus formation, making it difficult to produce high-activity calendula extracts on a large scale.

Method used

By employing a specific combination of induction medium, subculture medium, and suspension medium, including 6-benzylaminopurine and naphthaleneacetic acid, combined with MS, WPM, and DCR media, and optimizing culture conditions such as pH and culture time, a stable suspension culture system was established to improve the quality and proliferation efficiency of callus tissue.

Benefits of technology

It significantly improves the yield and purity of flavonoids, triterpenoids, and polyphenols in calendula callus tissue, exhibits excellent batch stability, is not limited by natural environment, is suitable for industrial production, and provides highly effective skin active ingredients for multifunctional skin products.

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Abstract

The invention provides a composition for calendula callus culture and application thereof. The composition comprises an induction culture medium, a subculture medium and a suspension culture medium, the induction culture medium is prepared from the following components according to final concentration: 0.1 to 0.5 mg / L of 6-benayl aminopurine and 2 to 4 mg / L of naphthylacetic acid; the subculture medium is prepared from the following components according to final concentration: 0.1 to 0.5 mg / L of 6-benayl aminopurine and 2 to 4 mg / L of naphthylacetic acid; the suspension culture medium is prepared from the following components according to final concentration: 0.1 to 0.2 mg / L of 6-benayl aminopurine and 1 to 3 mg / L of naphthylacetic acid; the basic culture medium is at least one of an MS culture medium, a WPM culture medium and a DCR culture medium. By exploring a proper callus induction hormone combination and constructing a mature suspension culture system, the callus quality and the suspension cell proliferation efficiency are remarkably improved, and a reliable technical basis is provided for large-scale production of active ingredients of calendula.
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