Formula and process of S-methoprene-containing fly attractant for pasture

Abstract

The invention relates to the technical field of mosquito and fly prevention and control, in particular to a formula and a process of an S-methoprene-containing fly attractant for a pasture. The insecticidal composition is prepared from the following raw materials: S-methoprene raw medicine, lure, a saccharide lure base, a protein source, ascorbic acid, titanium dioxide, a thickening suspending agent, a preservative, deionized water and dye. According to the invention, through step-by-step feeding and high-speed shearing, a stable viscous suspension system is constructed, photosensitive and volatile active components are effectively protected, and the lasting time of the drug effect is prolonged; the final product is pasty or gelatinous, so that targeted spot application in a pasture environment is facilitated, and environmental pollution is reduced. According to the technology, sex pheromone attraction, food source attraction and an insect growth regulator killing mechanism are successfully combined, an efficient, lasting and environment-friendly mosquito and fly prevention and control solution is provided, and the problems of drug resistance, drug residues and environmental pollution in traditional chemical agent prevention and control are effectively solved.

Description

Technical Field

[0001] This invention relates to the field of mosquito and fly control technology, and more specifically, to a formulation and process for a fly attractant containing S-acetamiprid for use in pastures. Background Technology

[0002] Mosquito and fly control is a comprehensive process of preventing and controlling the breeding and disease transmission of disease-carrying organisms such as mosquitoes and flies. The core of this approach is to reduce mosquito and fly density through environmental management, chemical and physical methods, thereby blocking the transmission routes of infectious diseases.

[0003] Fly infestation is a significant biological factor hindering the healthy development of the dairy farming industry. Studies have shown that flies can carry more than 70 pathogens, including major animal diseases such as foot-and-mouth disease, anthrax, and bovine nodular dermatitis, while also significantly increasing the incidence of common diseases like mastitis and calf diarrhea. Observations have shown that when the number of flies around a single cow reaches 150, about 1 / 30 of the individuals will exhibit feed-throwing behavior, resulting in daily feed waste of up to 75 kg. More seriously, infestations of houseflies and stable flies can lead to a 3.3% and 5% decrease in milk production, respectively. A 2016 international study showed that stable flies can cause an annual milk production loss of up to 139 kg per cow, and domestic farms have recorded typical cases of a 33% decrease in milk production in September compared to June.

[0004] Currently, mosquito and fly control in dairy farms mainly relies on environmentally residual chemical spraying and feed additives. However, traditional chemical control methods are facing serious challenges: on the one hand, feed additives such as cyprofen have been banned due to their conversion into harmful substances in the body; on the other hand, stable flies are developing increasing resistance to pesticides, leading to pesticide usage increasing to more than 10 times the historical level. Existing environmental disinfection is still limited to traditional chemical agents. This single-mode control not only exacerbates the development of resistance but also causes multiple problems such as drug residues, environmental pollution, and health risks to dairy cows. Summary of the Invention

[0005] The purpose of this invention is to avoid the problems caused by the limitation of environmental disinfection in existing mosquito and fly control to traditional chemical agents, which exacerbates the development of mosquito and fly resistance and causes multiple problems such as drug residues, environmental pollution and health risks to dairy cows.

[0006] The purpose of this invention is to provide a fly attractant formulation and process containing S-tebufenozide for use in ranches. By using the attractant in a relatively good environment, flies are attracted to prevent them from disturbing livestock. Furthermore, S-tebufenozide is used in conjunction with the fly attractant for biological population control.

[0007] To achieve the above objectives, one objective of this invention is to provide a fly attractant formulation containing S-tebufenozide for use in pastures, comprising the following raw materials in weight percentages: S-tebufenozide technical grade 0.8-1.2%, tinder 0.08-0.12%, saccharide attractant 40-50%, protein source 5-10%, ascorbic acid 0.8-1.2%, titanium dioxide 0.4-0.6%, thickening and suspending agent 1-2%, preservative 0.1-0.3%, with the balance being deionized water and dye.

[0008] As a further improvement to this technical solution, the sugar attractant is one or more of molasses or corn syrup, and the protein source is one or more of yeast powder or hydrolyzed protein.

[0009] As a further improvement to this technical solution, the thickening suspending agent is one or more of xanthan gum or magnesium aluminum silicate.

[0010] As a further improvement to this technical solution, the preservative is potassium sorbate and the dye is edible red pigment.

[0011] The second objective of this invention is to provide a process for preparing fly attractants using the aforementioned fly attractant formulation containing S-tebufenozide for ranches, comprising the following steps: Step S1: Weigh the raw materials according to the weight percentage, and then put S-tebufenozide technical, part of the thickening and suspending agent and part of the deionized water into a sand mill for wet grinding to obtain a micro-suspension for later use; Dissolve argentine in 1-2 times its weight of anhydrous ethanol and stir until completely dissolved. Then add ascorbic acid and titanium dioxide in sequence and continue stirring to disperse them evenly to obtain a mixture for later use. Step S2: Add the remaining deionized water to the main preparation vessel, start stirring and heat to 50-60℃; Add the carbohydrate inducer and protein source sequentially, stirring continuously until all components are completely dissolved to form a homogeneous glycoprotein solution; Step S3: Cool the above glycoprotein solution to below 40°C, add the micro-suspending agent, and stir to mix it evenly; Add the remaining thickening and suspending agent evenly, increase the stirring speed to 2000-3000 rpm, and perform high-speed shearing to form a viscous, stable, homogeneous system without visible particles; Step S4: Reduce the stirring speed to 100-200 rpm, add the mixture, then add the preservative and dye in sequence, and continue stirring until all components are evenly dispersed to obtain the attractant; After the attractant passes quality inspection, it is filled into a sealed tubing or a large-diameter squeeze bottle to obtain the finished product.

[0012] As a further improvement to this technical solution, in step S1, the grinding temperature is controlled to be below 40°C during wet grinding until the material particle size D90 < 10μm.

[0013] As a further improvement to this technical solution, in step S2, a glycosyl inducer and a protein source are added, and the stirring speed is maintained at 200-400 rpm until all components are completely dissolved.

[0014] As a further improvement to this technical solution, in step S3, when adding the micro-suspending agent, the stirring rate is 300-500 rpm, and the stirring time is 15-20 min.

[0015] As a further improvement to this technical solution, in step S3, the cutting time is 20-30 minutes.

[0016] As a further improvement to this technical solution, in step S4, after adding the preservative and dye, continue stirring for 10-15 minutes.

[0017] In this invention, wet grinding ensures the particle size of the core insecticidal ingredient, S-tebufenozide, facilitating fly transport. Stepwise feeding and high-speed shearing create a stable, viscous suspension system, effectively protecting photosensitizing and volatile active ingredients and extending the duration of efficacy. The final product is a paste or gel, allowing for targeted application in pasture environments and reducing environmental pollution. This process successfully combines sex pheromone attraction, food source attraction, and insect growth regulator killing mechanisms, providing a highly efficient, long-lasting, and environmentally friendly mosquito and fly control solution that effectively overcomes the problems of drug resistance, drug residues, and environmental pollution associated with traditional chemical pesticides.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: This fly attractant formulation and process for ranches, containing S-tebufenozide, uses high-purity S-tebufenozide as its core ingredient. It inhibits fly larvae pupation by mimicking juvenile hormones, fundamentally blocking population reproduction. A very low dose of pheromone is added as a pheromone attractant component to achieve precise attraction of target flies. The combination of sugars and protein sources mimics natural food sources and oviposition stimuli, significantly enhancing the attraction effect and feeding activity, ensuring sufficient agent delivery. Simultaneously, antioxidant and photostable components are added to ensure the stability of the active substances during processing and storage. Furthermore, S-tebufenozide is prepared into a micro-suspension by wet grinding, increasing the specific surface area to facilitate fly carrying. Then, a stable and viscous suspension system is formed through temperature-controlled shearing, which effectively suspends particles and delays volatilization. Finally, it is mixed at low temperature to form a paste or gel attractant, which can be applied to fly-gathering areas of the pasture. Through the "attraction-carrying-control breeding" mechanism, the use of chemical agents is significantly reduced, thus reducing the impact on dairy cows and the environment. Attached Figure Description

[0019] Figure 1 This is a flowchart of the present invention. Detailed Implementation

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] In the dairy farming industry, mosquito and fly control is often neglected. Traditional control methods mainly involve the extensive use of chemical agents for residual spraying in the farming area, or the addition of substances like cyprofen to the feed to suppress fly populations. However, with increasingly stringent requirements for pesticide residue monitoring and the growing severity of stable fly infestations, the amount of pesticides used has surged, leading to strong resistance. Currently, cyprofen has been banned due to its safety concerns, and environmental pest control remains largely in the traditional chemical control stage.

[0022] Houseflies and stable flies are the main fly species affecting livestock on pastures. Studies have shown that flies can carry more than 70 pathogens, including bacteria, viruses, and parasites. Houseflies have the habit of eating, regurgitating, and defecating simultaneously, spreading pathogens through mucous membranes and food contact; stable flies can pierce the skin of dairy cows to suck blood, spreading diseases as they change blood-sucking hosts. Diseases that flies can transmit include foot-and-mouth disease, anthrax, bovine nodular dermatitis, bovine ephemeral fever, porcine parvovirus, infectious keratoconjunctivitis, and avian influenza. They can also increase the incidence of common diseases such as mastitis, calf diarrhea, and hoof diseases (causing huddling and prolonged standing).

[0023] To avoid harassment from mosquitoes and flies, dairy cows exhibit various repelling behaviors, including tail wagging, head shaking, ear flapping, skin trembling, and leg lifting. Cows find it difficult to repel flies on their backs using these behaviors, so they resort to tossing feed to scare them away. However, this feeding-throwing behavior can also cause losses for the farm. The more flies there are on the farm, the more cows will exhibit this behavior. According to relevant data, when there are 1-150 flies around a cow, 1 / 30 of the cows will learn to throw feed, resulting in a daily feed waste of up to 75 kg.

[0024] The infestation of mosquitoes and flies by dairy cows also affects their milk production. Due to the bites and harassment from mosquitoes and flies, dairy cows experience discomfort and stress, leading to decreased feed intake. Especially when stable fly populations are high, the cows' defensive behavior changes from repelling to gathering, resulting in reduced feeding and resting time. Simultaneously, the stress response caused by mosquitoes and flies depletes the cows' energy, further impacting milk production. Studies have reported a linear correlation between stable fly populations and tail-whipping, skin tremors, head-whipping, and leg-lifting behaviors in dairy cows. Tail-whipping has the highest frequency, followed by skin tremors, with head-whipping and leg-lifting occurring third and fourth most frequently. The more frequent the animal's defensive behavior, the greater the impact on its normal feeding, drinking, resting, standing, and walking behaviors.

[0025] Studies have reported that houseflies reduce dairy production by 3.3%, while stable flies reduce it by up to 5%. A 2016 study in the *Journal of Medical Entomology* estimated the average annual milk production loss per cow due to stable flies to be 139 kg. A 2010 domestic study reported that a dairy farm in Inner Mongolia, affected by houseflies and stable flies, experienced significantly lower average milk production in September compared to June, July, and August; compared to June, milk production in July, August, and September decreased by 1%, 4%, and 33%, respectively.

[0026] Previously, fly control in pastures involved adding cyprofen to feed. However, long-term use rendered cyprofen ineffective, requiring doses 10 times higher or more to be effective. Currently, cyprofen is banned because it transforms into harmful substances in livestock. Environmental disinfection still relies on traditional chemical agents, which will inevitably lead to a surge in drug resistance in the long run.

[0027] Therefore, one of the objectives of this invention is to provide a fly attractant formulation containing S-tebufenozide for use in pastures, comprising the following raw materials in weight percentages: S-tebufenozide technical grade 0.8-1.2%, tinder 0.08-0.12%, saccharide attractant 40-50%, protein source 5-10%, ascorbic acid 0.8-1.2%, titanium dioxide 0.4-0.6%, thickening and suspending agent 1-2%, preservative 0.1-0.3%, with the balance being deionized water and dye.

[0028] Furthermore, the sugar attractant is one or more of molasses or corn syrup, the protein source is one or more of yeast powder or hydrolyzed protein, the thickening and suspending agent is one or more of xanthan gum or magnesium aluminum silicate, the preservative is potassium sorbate, and the dye is edible red pigment.

[0029] In this invention, S-tebufenozide technical grade, as an insect growth regulator, effectively inhibits the pupation and normal development of fly larvae by mimicking juvenile hormones; pheromone, as a highly efficient pheromone attractant, specifically attracts male and female flies to the application site, enhancing the targeted effect. The sugar attractant is molasses or corn syrup, which provides flies with a favorite food source to stimulate feeding and serves as a sticky matrix and humectant for the formulation; the protein source is yeast powder or hydrolyzed protein, which significantly enhances the attraction to female flies during the egg-laying period and prompts them to carry S-tebufenozide to the breeding site. Ascorbic acid acts as an antioxidant to prevent the attractant from oxidizing and becoming ineffective during formulation and storage; titanium dioxide acts as a light stabilizer to effectively block ultraviolet rays and protect the photosensitivity of S-tebufenozide and tebufenozide. The thickening and suspending agent is xanthan gum or magnesium aluminum silicate, which constructs a uniform and stable suspension system, prevents solid particles from settling and ensures that the formulation has suitable adhesion; the preservative is potassium sorbate, which prevents the high sugar system from becoming moldy and extends the shelf life of the product in humid environments. The dye is an edible red pigment, serving as a visual warning and location marker; deionized water, as an environmentally friendly carrier, provides good dispersibility and processing adaptability.

[0030] Please see Figure 1 As shown, a second objective of this invention is to provide a process for preparing a fly attractant using the above-mentioned fly attractant formulation containing S-tebufenozide for ranches, comprising the following steps: Step S1: Weigh the raw materials according to the weight percentage, and then put the S-tebufenozide technical, part of the thickening and suspending agent (accounting for 30-50% of the total amount) and part of the deionized water into a sand mill for wet grinding. During wet grinding, control the grinding temperature to be below 40℃ until the material particle size D90 < 10μm, and obtain the micro-suspension agent for later use.

[0031] Dissolve argentin in 1-2 times its weight of anhydrous ethanol and stir until completely dissolved. Then add ascorbic acid and titanium dioxide in sequence and continue stirring to disperse them evenly to obtain a mixture for later use.

[0032] Step S2: Add the remaining deionized water to the main preparation vessel, start stirring and heat to 50-60℃.

[0033] Add the glycosyl inducer and protein source sequentially, maintaining a stirring speed of 200-400 rpm until all components are completely dissolved, forming a homogeneous glycoprotein solution.

[0034] Step S3: Cool the above glycoprotein solution to below 40°C, slowly add the micro-suspending agent, and stir until it is evenly mixed. It is worth noting that when adding the micro-suspending agent, the stirring speed should be 300-500 rpm, and stirring should be carried out for 15-20 minutes.

[0035] Slowly and evenly add the remaining thickening suspending agent, increase the stirring speed to 2000-3000 rpm, and perform high-speed shearing for 20-30 minutes to ensure that the thickening agent is fully swollen and evenly dispersed to form a viscous, stable, homogeneous system without visible particles.

[0036] Step S4: Reduce the stirring speed to 100-200 rpm, slowly add the mixture, then add the preservative and dye in sequence, and continue stirring at low speed for 10-15 minutes until all components are fully dispersed and there are no excessive bubbles in the system, to obtain a paste or gel attractant.

[0037] The attractant undergoes quality testing, with key indicators including viscosity, pH value, and suspension stability. Once qualified, it is filled into sealed tubing or large-diameter squeeze bottles to obtain the finished product.

[0038] The following specific embodiments further illustrate the formulation and process of a fly attractant containing S-acetamiprid for ranches provided by the present invention.

[0039] Example 1 Step S1: Weigh out the following by weight percentage: 0.8% S-tebufenozide technical grade, 0.12% tectonic ether, 40% saccharide attractant, 10% protein source, 0.8% ascorbic acid, 0.6% titanium dioxide, 1% thickening and suspending agent, 0.3% preservative, with the remainder being deionized water and dye. The sugar attractant is molasses, the protein source is yeast powder; the thickening and suspending agent is xanthan gum; the preservative is potassium sorbate, and the dye is edible red food coloring. Then, S-tebufenozide technical, a portion of thickening and suspending agent (accounting for 30-50% of the total amount) and a portion of deionized water are put into a sand mill for wet grinding. During wet grinding, the grinding temperature is controlled to be below 40℃ until the particle size D90 of the material is <10μm, and a micro-suspension agent is obtained for later use.

[0040] Dissolve argentin in anhydrous ethanol at one times its weight and stir until completely dissolved. Then add ascorbic acid and titanium dioxide in sequence and continue stirring to disperse them evenly to obtain a mixture for later use.

[0041] Step S2: Add the remaining deionized water to the main preparation vessel, start stirring and heat to 50°C.

[0042] Add the glycosyl inducer and protein source sequentially, maintaining a stirring speed of 400 rpm, until all components are completely dissolved to form a homogeneous glycoprotein solution.

[0043] Step S3: Cool the above glycoprotein solution to below 40°C, add the micro-suspending agent, and stir until homogeneous. It is important to note that when adding the micro-suspending agent, the stirring speed should be 300 rpm for 20 minutes.

[0044] Add the remaining thickening and suspending agent evenly, increase the stirring speed to 2000 rpm, and perform high-speed shearing for 30 minutes to form a viscous, stable, homogeneous system without visible particles.

[0045] Step S4: Reduce the stirring speed to 100 rpm, add the mixture, then add the preservative and dye in sequence, and continue stirring for 15 minutes until all components are evenly dispersed to obtain the attractant.

[0046] After the attractant passes quality inspection, it is filled into a sealed tubing or a large-diameter squeeze bottle to obtain the finished product.

[0047] Example 2 Step S1: Weigh out the following by weight percentage: 1.0% of S-tebufenozide technical, 0.10% of tectonic ether, 45% of saccharide attractant, 8% of protein source, 1.0% of ascorbic acid, 0.5% of titanium dioxide, 1% of thickening and suspending agent, 0.2% of preservative, with the remainder being deionized water and dye. The sugar attractants are molasses and corn syrup; the protein sources are yeast powder and hydrolyzed protein; the thickening and suspending agents are xanthan gum and magnesium aluminum silicate; the preservative is potassium sorbate; and the dye is edible red food coloring. Then, S-tebufenozide technical, a portion of thickening and suspending agent (accounting for 30-50% of the total amount) and a portion of deionized water are put into a sand mill for wet grinding. During wet grinding, the grinding temperature is controlled to be below 40℃ until the particle size D90 of the material is <10μm, and a micro-suspension agent is obtained for later use.

[0048] Dissolve argentin in anhydrous ethanol at one times its weight and stir until completely dissolved. Then add ascorbic acid and titanium dioxide in sequence and continue stirring to disperse them evenly to obtain a mixture for later use.

[0049] Step S2: Add the remaining deionized water to the main preparation vessel, start stirring and heat to 55°C.

[0050] Add the glycosyl inducer and protein source sequentially, maintaining a stirring speed of 300 rpm, until all components are completely dissolved to form a homogeneous glycoprotein solution.

[0051] Step S3: Cool the above glycoprotein solution to below 40°C, add the micro-suspending agent, and stir until homogeneous. It is worth noting that when adding the micro-suspending agent, the stirring speed should be 400 rpm for 17 minutes.

[0052] Add the remaining thickening and suspending agent evenly, increase the stirring speed to 2500 rpm, and perform high-speed shearing for 25 minutes to form a viscous, stable, homogeneous system without visible particles.

[0053] Step S4: Reduce the stirring speed to 150 rpm, add the mixture, then add the preservative and dye in sequence, and continue stirring for 12 minutes until all components are evenly dispersed to obtain the attractant.

[0054] After the attractant passes quality inspection, it is filled into a sealed tubing or a large-diameter squeeze bottle to obtain the finished product.

[0055] Example 3 Step S1: Weigh out the following by weight percentage: 1.2% S-tebufenozide technical, 0.08% tectonic ether, 50% saccharide attractant, 5% protein source, 1.2% ascorbic acid, 0.4% titanium dioxide, 2% thickening and suspending agent, 0.1% preservative, with the remainder being deionized water and dye. The carbohydrate attractant is corn syrup, the protein source is hydrolyzed protein, the thickening and suspending agent is magnesium aluminum silicate, the preservative is potassium sorbate, and the dye is edible red food coloring. Then, S-tebufenozide technical, a portion of thickening and suspending agent (accounting for 30-50% of the total amount) and a portion of deionized water are put into a sand mill for wet grinding. During wet grinding, the grinding temperature is controlled to be below 40℃ until the particle size D90 of the material is <10μm, and a micro-suspension agent is obtained for later use.

[0056] Dissolve argentin in anhydrous ethanol at twice its weight and stir until completely dissolved. Then add ascorbic acid and titanium dioxide in sequence and continue stirring to disperse them evenly to obtain a mixture for later use.

[0057] Step S2: Add the remaining deionized water to the main preparation vessel, start stirring and heat to 60°C.

[0058] Add the glycosyl inducer and protein source sequentially, maintaining a stirring speed of 200 rpm, until all components are completely dissolved to form a homogeneous glycoprotein solution.

[0059] Step S3: Cool the above glycoprotein solution to below 40°C, add the micro-suspending agent, and stir until homogeneous. It is important to note that when adding the micro-suspending agent, the stirring speed should be 500 rpm for 15 minutes.

[0060] Add the remaining thickening and suspending agent evenly, increase the stirring speed to 3000 rpm, and perform high-speed shearing for 20 minutes to form a viscous, stable, homogeneous system without visible particles.

[0061] Step S4: Reduce the stirring speed to 200 rpm, add the mixture, then add the preservative and dye in sequence, and continue stirring for 10 minutes until all components are evenly dispersed to obtain the attractant.

[0062] After the attractant passes quality inspection, it is filled into a sealed tubing or a large-diameter squeeze bottle to obtain the finished product.

[0063] The attractant was prepared according to Examples 1-3, and then the attractant was subjected to fly emergence inhibition test.

[0064] The specific testing method is as follows: In a constant-temperature insect rearing room with a temperature of 28±2℃, humidity of 65%±10%, and a photoperiod of 12 hours of light / 12 hours of darkness, construct one or more cubic mesh covers with dimensions of 1m (length) × 1m (width) × 1m (height).

[0065] Prepare two identical, open plastic or glass petri dishes (about 9cm in diameter) as food trays.

[0066] Treatment groups 1-3: Weigh 5.0g of the S-acetyl ester attractant (paste) prepared in Examples 1-3 and place it in a bait tray.

[0067] Control group: Weigh 5.0g of blank glycoprotein matrix containing no active ingredients (S-tebufenozide, tebufenozide) and place it in another feed dish.

[0068] Two hundred healthy and active adult houseflies, 3-5 days old after emergence, were collected from a standard laboratory strain, with a male-to-female ratio of approximately 1:1. These houseflies were starved for 4-6 hours prior to the experiment.

[0069] Two bait trays (treatment groups 1-3 and the control group) were placed in opposite corners of the enclosure, maintaining a straight-line distance of approximately 1.5 meters to avoid odor interference. Two hundred houseflies, subjected to starvation treatment, were gently released into the center of the enclosure through an opening at the top. At 1, 2, 4, 8, and 24 hours after release, the number of flies remaining on each bait tray and within its surrounding area (approximately 3 cm) was observed and recorded. Each count lasted 5 minutes and was recorded using a camera.

[0070] After 24 hours of observation, a small oviposition cup containing a standard oviposition substrate (such as a mixture of moist wheat bran and milk powder) was provided inside the enclosure to induce surviving houseflies to lay eggs. The oviposition cup was removed after 6 hours. The collected eggs (or newly hatched larvae) were transferred to a culture box containing fresh larval feed and reared under standard conditions (28℃, 70-80% RH). Once the larval development period was complete, the number of individuals that successfully emerged as adults was counted, and the inhibition rate was calculated (inhibition rate = (number of adults emerging in the control group - number of adults emerging in the treatment group) / number of adults emerging in the control group × 100%). The results are recorded in Table 1.

[0071] Table 1. Inhibition effect of the attractants prepared in Examples 1-3 on fly emergence.

[0072] As shown in Table 1, the fly emergence inhibition rate of the attractants prepared in Examples 1-3 is not less than 91.8%, indicating that the fly attractant formula and process containing S-tebufenozide for ranches provided by the present invention can prepare attractants with good fly emergence inhibition effect.

[0073] In this invention, high-purity S-tebufenozide technical grade is used as the core active ingredient. It effectively inhibits fly maggot pupation by mimicking insect juvenile hormones, thus blocking population reproduction at the source. A very low dose of pheromone is added as a highly effective pheromone attractant component, achieving precise attraction to target flies and significantly improving the targeting effect. Furthermore, a saccharide attractant, in conjunction with a protein source, mimics the natural food and oviposition stimuli of flies, greatly enhancing the attraction and feeding activity of adult flies (especially female flies that require protein supplementation), ensuring they are fully contaminated and carry the pesticide. Simultaneously, the addition of antioxidants and light-blocking agents effectively protects the chemical and photochemical stability of S-tebufenozide and pheromone during processing, storage, and use, ensuring the product's long-lasting effect. In addition, wet grinding is used to process S-tebufenozide technical into a micro-suspension with a D90 < 10 μm, which greatly increases the specific surface area of ​​the particles, facilitating adhesion and carrying by flies. Then, through temperature-controlled dissolution and high-speed shearing processes, a viscous suspension system with xanthan gum or magnesium aluminum silicate as stabilizers is constructed. This system can not only uniformly suspend active particles and prevent sedimentation and stratification, but also has suitable adhesiveness, delays the volatilization of the attractant, and prolongs the duration of effect. Finally, photostable and antioxidant components are mixed at low temperature to form a uniform and stable paste or gel attractant, which can be applied to areas where flies gather, such as pasture fences and walls. Through the mechanism of "attracting-carrying-inhibiting reproduction", it effectively reduces the use of chemical agents and reduces the negative impact on dairy cows and the environment.

[0074] Test case This experiment was conducted in livestock farming areas such as dairy cow sheds, poultry houses, and pig pens where flies congregate. The bait was applied primarily to vertical surfaces where flies prefer to land, such as support pillars, walls, railings, and the edges of feed troughs (avoiding direct application to feed or areas where animals can lick it). A coin-sized (approximately 3-5 grams) dotted bait was applied every 5-10 meters, or a dedicated attractant was used to place the bait inside the attractant (replenishment is generally required every 4-8 weeks, depending on product durability and fly pressure. Increased inspection frequency is necessary during rainy seasons or in high-temperature environments).

[0075] The following are the attraction test data and progeny emergence inhibition data for different concentrations of the attractant used in this invention.

[0076] Fifty flies were randomly introduced into a sealed connector. One side of the connector contained an attractant prepared according to the formula described above, with the following concentration: Formula 1: S-acetamiprid content 0.1%, chlorpyrifos content 0.01%.

[0077] Formula 2: S-acetamiprid content 0.5%, chlorpyrifos content 0.05%.

[0078] Formula 3: S-acetamiprid content 1%, acetamiprid content 0.1%.

[0079] The above three formulas are identical except for the main ingredient.

[0080] Formulas 4, 5, and 6: These three formulas are independent attractants on the other side of the connector that do not contain S-tebufenozide, but only tebufenozide. They also do not contain protein sources or sugar attractants. The tebufenozide content is 0.01%, 0.05%, and 0.1%, respectively.

[0081] The pesticides were spread evenly on sticky cardboard. The sticky cardboard was used to restrict the flight of flies once they landed, preventing cross-contamination between the two pesticides. The experiment lasted 30 minutes and was repeated 3 times. The observed data are shown in Tables 2 and 3.

[0082] Table 2. Attractant properties of different contents of pheromone

[0083] Table 3. Attraction and feathering inhibition tests of products with different formulations

[0084] Table 2 shows that the higher the attractant content of independent pheromone, the more obvious the effect, with the smallest difference between 0.01% and 0.05%.

[0085] As shown in Table 2, when combined with other attractants, such as protein sources and sugars in the formula, pheromone can greatly enhance the attractiveness of the product.

[0086] After removing the flies from the sticky board in Experiment 2, they were kept in an incubator to observe the emergence and reproduction of their offspring. It was found that the emergence rate of the offspring larvae (maggots) of flies in formulas 1, 2, and 3 was less than 3%, while the emergence rate of the offspring larvae (maggots) of flies in formulas 4, 5, and 6 was over 95%.

[0087] In summary, S-acetamiprid significantly inhibited fly emergence at the concentration gradient with little difference, indicating that S-acetamiprid has extremely high activity.

[0088] The individual attraction of pheromone is not as high as that of mixed attractants.

[0089] The combination of S-tebufenozide and chlorantraniliprole can indeed effectively suppress fly populations and greatly reduce the use of chemical agents.

[0090] In summary, this fly attractant achieves highly efficient attraction and control of houseflies and stable flies through the triple synergistic effect of pheromone (sex pheromone), sugar and protein sources (food source), and S-acetamiprid (insecticide). Its mechanism of action is as follows: first, the pheromone and food odor attract adult flies from a distance to land and feed, causing S-acetamiprid microparticles to adhere to their body surface, making them "mobile pesticide carriers." Then, the pesticide is transferred to the breeding environment through oviposition by female flies or contact between individuals. Finally, S-acetamiprid inhibits the pupation of larvae, blocking population reproduction at the root, significantly reducing the amount of chemical pesticides used. It has both high efficiency and environmental safety, making it suitable for large-scale ranch applications.

[0091] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A process for using an S-acetamiprid-containing fly attractant for ranches, characterized in that, Includes the following steps: Step S1: Weigh out the following by weight percentages: 0.8-1.2% S-tebufenozide technical, 0.08-0.12% tartrate, 40-50% saccharide attractant, 5-10% protein source, 0.8-1.2% ascorbic acid, 0.4-0.6% titanium dioxide, 1-2% thickening suspending agent, and 0.1-0.3% preservative. The remainder is deionized water and dye. Then, put the S-tebufenozide technical, part of the thickening suspending agent, and part of the deionized water into a sand mill for wet grinding to obtain a micro-suspension for later use. Dissolve argentine in 1-2 times its weight of anhydrous ethanol and stir until completely dissolved. Then add ascorbic acid and titanium dioxide in sequence and continue stirring to disperse them evenly to obtain a mixture for later use. Step S2: Add the remaining deionized water to the main preparation vessel, start stirring and heat to 50-60℃; Add the carbohydrate inducer and protein source sequentially, stirring continuously until all components are completely dissolved to form a homogeneous glycoprotein solution; Step S3: Cool the above glycoprotein solution to below 40°C, add the micro-suspending agent, and stir to mix it evenly; Add the remaining thickening and suspending agent evenly, increase the stirring speed to 2000-3000 rpm, and perform high-speed shearing to form a viscous, stable, homogeneous system without visible particles; Step S4: Reduce the stirring speed to 100-200 rpm, add the mixture, then add the preservative and dye in sequence, and continue stirring until all components are evenly dispersed to obtain the attractant; After the attractant passes quality inspection, it is filled into a sealed tubing or a large-diameter squeeze bottle to obtain the finished product.

2. The process for using an S-acetamiprid-containing fly attractant for ranches according to claim 1, characterized in that: In step S1, the sugar attractant is one or more of molasses or corn syrup, and the protein source is one or more of yeast powder or hydrolyzed protein.

3. The process for using an S-tebufenozide fly attractant for ranches according to claim 1, characterized in that: In step S1, the thickening suspending agent is one or more of xanthan gum or magnesium aluminum silicate.

4. The process for using an S-tebufenozide fly attractant for ranches according to claim 1, characterized in that: In step S1, the preservative is potassium sorbate, and the dye is edible red pigment.

5. The process for using an S-tebufenozide fly attractant for ranches according to claim 1, characterized in that: In step S1, the grinding temperature is controlled to be below 40°C during wet grinding until the particle size D90 of the material is less than 10μm.

6. The process for using an S-acetamiprid-containing fly attractant for ranches according to claim 1, characterized in that: In step S2, sugar inducers and protein sources are added, and the stirring speed is maintained at 200-400 rpm until all components are completely dissolved.

7. The process for using an S-tebufenozide fly attractant for ranches according to claim 1, characterized in that: In step S3, when adding the micro-suspending agent, the stirring speed is 300-500 rpm, and the stirring time is 15-20 min.

8. The process for using an S-acetamiprid-containing fly attractant for ranches according to claim 1, characterized in that: In step S3, the cutting time is 20-30 minutes.

9. The process for using an S-acetamiprid-containing fly attractant for ranches according to claim 1, characterized in that: In step S4, after adding the preservative and dye, continue stirring for 10-15 minutes.

10. The fly attractant formulation containing S-tebufenozide for ranches according to any one of claims 1-9, characterized in that, Including the following raw materials: S-tebufenozide technical grade, pheromone, saccharide attractant, protein source, ascorbic acid, titanium dioxide, thickener / suspending agent, preservative, dye, and deionized water, wherein: The S-tebufen technical material, as an insect growth regulator, inhibits fly maggot pupation and normal development by mimicking juvenile hormones; the insect attractant, as a pheromone attractant, specifically attracts target flies to the application area. The glycosyl attractant provides food source stimulation and serves as a sticky matrix for the formulation; the protein source enhances attraction to female flies during the oviposition period, promoting their carrying of the agent to the breeding ground; the ascorbic acid acts as an antioxidant, preventing the active ingredient from oxidizing and becoming ineffective; and the titanium dioxide acts as a light stabilizer, inhibiting the photodecomposition of the active ingredient. The thickening and suspending agent is used to construct a stable suspension system and prevent particle sedimentation; the preservative prevents mold growth in the high-sugar system; the dye provides visual warnings and location markings; and the deionized water serves as an environmentally friendly carrier.

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