Method for treating knee osteoarthritis by combining PRP with PPP periknee acupoint injection
By combining PRP with PPP injection at acupoints around the knee, local repair and overall regulation of knee osteoarthritis can be achieved, overcoming the shortcomings of existing treatment methods and providing a safe, effective, and economical treatment option.
Patent Information
- Application Number
- CN202511493427.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-20
- Publication Date
- 2025-12-16
AI Technical Summary
Existing treatments for knee osteoarthritis have problems such as significant side effects, poor compliance, high costs, and high risks. Simple PRP intra-articular injection has limited efficacy, while traditional Chinese medicine autologous blood therapy has imprecise components and unclear mechanisms of action.
The method of combining PRP and PPP injection into acupoints around the knee is adopted. PRP is injected into the knee joint cavity, and PPP is injected into acupoints around the knee, including acupoints such as Dubi, Xuehai, and Zusanli. Through the synergistic effect of local repair and overall regulation, cartilage repair and microcirculation improvement are achieved.
It significantly relieves pain, improves joint function, increases cartilage thickness, is highly safe, easy to operate, suitable for promotion in primary hospitals, has low cost, and is more effective than PRP treatment alone.
Smart Images
Figure CN121129897A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The application belongs to the technical field of regenerative medicine, and particularly relates to a method for treating knee osteoarthritis by PRP combined with PPP knee periarthritis acupoint injection. BACKGROUND
[0002] Knee osteoarthritis (KOA) is a common chronic degenerative disease in the elderly, and is clinically manifested as knee joint pain, limited activity and dysfunction. With the aggravation of population aging, the incidence of KOA is increasing year by year, and has become one of the major disabling diseases in the world.
[0003] The existing treatment methods mainly include: Drug treatment: such as non-steroidal anti-inflammatory drugs, but there are problems of large side effects and limited long-term use; Physical therapy: such as rehabilitation training and physiotherapy, but with slow effect and poor compliance; Surgical treatment: such as joint replacement, which is high in cost and high in risk, and is only suitable for patients in the late stage.
[0004] In recent years, platelet-rich plasma (PRP) has been applied to the treatment of osteoarthritis as a regenerative medicine material, which can promote the repair of articular cartilage and reduce inflammatory reactions. However, the effect of simple PRP intra-articular injection is limited, and it is difficult to achieve overall regulation. The traditional Chinese medicine "autologous blood therapy" mainly uses autologous whole blood or serum for acupoint injection, and has problems of inaccurate composition and unclear mechanism of action.
[0005] Therefore, it is urgent to provide a new treatment method combining the advantages of accurate repair of modern regenerative medicine and overall regulation of traditional Chinese medicine meridians to overcome the shortcomings of the prior art. SUMMARY
[0006] To overcome the above technical problems, the application provides a method for treating knee osteoarthritis by PRP combined with PPP knee periarthritis acupoint injection, which realizes the synergistic effect of "local repair + overall regulation", effectively relieves pain, improves knee joint function, delays disease progression, and has the advantages of safety, simplicity and easy promotion.
[0007] The application adopts the following technical scheme: The method for treating knee osteoarthritis by PRP combined with PPP knee periarthritis acupoint injection comprises the following steps: Collecting venous blood of a patient and separating platelet-rich plasma (PRP) and platelet-poor plasma (PPP) by single centrifugation; Injecting the PRP into the knee joint cavity, once a week for 5 times; Injecting the PPP into knee periarthritis acupoints, including the acupoints of Du'niu, Xuehai and Zusanli, 1ml per acupoint, once a week for 5 times; Among them, the platelet concentration of PRP is 1000-1200×10⁻⁶. 9 / L, PPP platelet concentration less than 200×10 9 / L.
[0008] Preferably, the blood collection volume is 10 ml, and blood is collected using a non-anticoagulating screw-in syringe.
[0009] Preferably, the single centrifugation method includes: Centrifuge at 0 degrees Celsius in a low-temperature centrifuge at a speed of 3000 rpm for 10 minutes.
[0010] Preferably, PPP acupoint injection adopts the theory of local acupoint selection and meridian differentiation.
[0011] Preferably, the efficacy is evaluated using VAS pain score, Lysholm functional score, and MRI imaging.
[0012] Compared with the prior art, the beneficial effects of the present invention are: Compared to existing treatments, the biggest advantage of this "PRP+PPP" combined therapy is that it can directly repair cartilage and reduce inflammation within the joint cavity, while also improving microcirculation and regulating the overall condition through acupoints around the knee. This combination of internal and external approaches results in faster pain relief, more significant recovery of joint function, and an observed increase in cartilage thickness.
[0013] This method has excellent safety, few adverse reactions, and simple operation steps, making it suitable for implementation in primary hospitals. Overall, it is more economical and practical than simple drug or surgical treatment. Single-stage low-temperature centrifugation, without the addition of anticoagulants or separation gels, reduces the occurrence of adverse reactions and lowers costs, making it suitable for widespread application. Attached Figure Description
[0014] Figure 1 This is a schematic diagram of the PRP / PPP preparation and injection process of the present invention; Figure 2 This is a schematic diagram of the synergistic mechanism of PRP+PPP in this invention. Detailed Implementation
[0015] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. Unless otherwise specified, the raw materials and equipment used are commercially available or commonly used in the art. The methods in the embodiments, unless otherwise specified, are conventional methods in the art. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.
[0016] I. Blood Collection and Preparation Methods Subjects: Patients with knee osteoarthritis (stage II-III).
[0017] Blood collection: Collect 10ml of venous blood and place it in a 10ml non-anticoagulated screw-in syringe.
[0018] Separation: A low-temperature, high-speed variable-speed centrifuge was used, employing a single-cycle centrifugation method. Centrifuge at 3000 rpm and 0 degrees Celsius for 10 minutes to obtain PRP and PPP.
[0019] Ingredient analysis: Blood routine test results: PRP platelet concentration is 4-5 times that of baseline whole blood, approximately 1000-1200 × 10⁻⁶. 9 / L; PPP platelet concentration less than 200×10 9 / L.
[0020] II. PRP Injection Procedure Injection site: Inside the knee joint cavity.
[0021] Injection method: The patient sits with knees bent at 90° and lower legs hanging naturally. The needle is inserted into the inner / outer knee eye. Disposable sterile syringes and needles are used throughout the procedure, and the area is disinfected with povidone-iodine before the procedure.
[0022] Dosage and course of treatment: PRP 1.5ml, once a week for a total of 5 times.
[0023] III. PPP Acupoint Injection Procedure Acupoint selection: Dubi (ST35), Xuehai (SP10), Zusanli (ST36).
[0024] Injection method: Use straight or oblique insertion, with a needle tip depth of 0.5-1 inch. After insertion, slowly push in PPP and instruct the patient to maintain the needling sensation.
[0025] Dosage and course of treatment: 1 ml of PPP per acupoint, once a week, for a total of 5 times.
[0026] IV. Clinical Study Design Study participants: Sixty female patients with KOA aged 50-70 years were included and randomly divided into two groups: Group A (control): PRP intra-articular injection alone; Group B (Experiment): PRP intra-articular injection + PPP acupoint injection.
[0027] Treatment course: All patients received 4 injections and were followed up for 6 months.
[0028] Observation indicators: VAS pain score, Lysholm functional score, SF-36 quality of life scale, MRI cartilage thickness, and serum inflammatory factor levels (IL-1β, TNF-α).
[0029] V. Test Results
[0030] The results show that: 1. Both groups showed improvement after treatment, but the PRP+PPP combined group was superior to the PRP alone group in terms of pain relief, functional recovery and cartilage thickness improvement (P<0.05).
[0031] 2. The more significant decrease in inflammatory factor levels indicates that PPP acupoint injection has a synergistic anti-inflammatory effect.
[0032] 3. No serious adverse reactions were observed throughout the process, and the incidence of adverse reactions was less than 5%.
[0033] Although embodiments of the present invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and variations can be made to the above embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the claims and their equivalents.
Claims
1. A method for treating knee osteoarthritis using PRP combined with PPP peri-knee acupoint injection, characterized in that, Includes the following steps: Patient venous blood was collected and platelet-rich plasma (PRP) and platelet-poor plasma (PPP) were separated by a single centrifugation method. The PRP is injected into the knee joint cavity once a week for five consecutive weeks. The PPP was injected into acupoints around the knee, including Dubi, Xuehai and Zusanli, 1ml per acupoint, once a week for 5 consecutive weeks. Among them, the platelet concentration of PRP is 1000-1200×10⁻⁶. 9 / L, PPP platelet concentration less than 200×10 9 / L.
2. The method for treating knee osteoarthritis by combining PRP with PPP peri-knee acupoint injection according to claim 1, characterized in that, The blood volume was 10 ml, and blood was collected using a non-anticoagulating screw-in syringe.
3. The method for treating knee osteoarthritis by combining PRP with PPP perineal acupoint injection according to claim 1, characterized in that, Single centrifugation methods include: Centrifuge at 0 degrees Celsius in a low-temperature centrifuge at a speed of 3000 rpm for 10 minutes.
4. The method for treating knee osteoarthritis by combining PRP with PPP peri-knee acupoint injection according to claim 1, characterized in that, PPP acupoint injection adopts the theory of local acupoint selection and meridian differentiation.
5. The method for treating knee osteoarthritis by combining PRP with PPP peri-knee acupoint injection according to claim 1, characterized in that, The efficacy was evaluated using VAS pain scores, Lysholm functional scores, and MRI imaging.