Biological fresh-keeping sticker as well as production method and application thereof

Abstract

A sticker includes at least one adhesive component comprising a carrier composition containing a nutritional composition; wherein the carrier composition comprises a food grade binder and the nutritional composition comprises at least one compound adapted to support selective growth of one or more human safe bacteria.

Description

Technical Field

[0001] This disclosure relates to methods and apparatus for preserving food, agricultural products, and other consumer goods. Background Technology

[0002] The following is a list of references considered relevant to the background art of the currently disclosed topic:

[0003] International Patent Application Publication No. WO2021 / 033190

[0004] The acknowledgment of the above references in this document should not be construed as implying that these references are in any way related to the patentability of the currently disclosed subject matter. Background Technology

[0006] The food industry is constantly searching for safe methods to preserve food and agricultural products.

[0007] WO2021 / 033190 describes a protective insert comprising a discrete substrate forming material that contains a nutrient composition comprising a nutrient combination for supporting the selective growth of one or more bacteriocinogenic bacteria on the substrate and is substantially free of externally added microorganisms. This protective insert can be included in consumer product packaging containing the article to be protected and can be contained within a carrier. Summary of the Invention

[0008] This disclosure is based on the development of a food preservation sticker for increasing the shelf life of consumer products, fresh food products, and agricultural products by stimulating the growth of selected bacteria within the packaging containing the article, without using live bacteria added externally to the consumer product, thereby eliminating the difficulties and costs associated with the use of live bacteria in industrial plants.

[0009] Therefore, according to a first aspect of the currently disclosed subject matter, a sticker is provided comprising at least one adhesive component, the at least one adhesive component comprising a carrier composition containing a nutrient composition; wherein the carrier composition comprises a food-grade adhesive, and the nutrient composition comprises at least one compound suitable for supporting the selective growth of one or more human-safe bacteria.

[0010] According to the second aspect of the currently disclosed subject matter, a consumer product or consumer product packaging and a sticker of the first aspect of the currently disclosed subject matter are provided, the sticker being adhered to the packaging element of the consumer product or containing the article.

[0011] According to a third aspect of the currently disclosed subject matter, a method for preserving consumer products is provided, the method comprising adhering a sticker from a first aspect of the currently disclosed subject matter to packaging containing the consumer product, the adhesion being performed in a manner that allows moisture channels between the consumer product and the adhesive component.

[0012] According to a fourth aspect of the currently disclosed subject matter, a method for manufacturing food safety stickers is provided, the method comprising mixing a carrier composition comprising a food-grade adhesive with a nutrient composition comprising at least one compound suitable for supporting the selective growth of one or more human-safe bacteria to form an adhesive assembly, and shaping the adhesive assembly.

[0013] Finally, according to the fifth aspect of the currently disclosed subject matter, a method for manufacturing a food safety sticker is provided, the method comprising: providing an adhesive assembly comprising a carrier composition containing a nutrient composition; wherein the carrier composition comprises a food-grade adhesive and the nutrient composition comprises at least one compound suitable for supporting the selective growth of one or more human-safe bacteria; and disposing at least one of a first backing material and a first removable liner to at least one side of the adhesive assembly, and optionally disposing at least one of a second backing material and a second removable liner to an opposite second side of the adhesive assembly, wherein the first backing material and the second backing material may be the same or different, and the first removable liner and the second removable liner may be the same or different. Attached Figure Description

[0014] To better understand the subject matter disclosed herein and to illustrate how it can be implemented in practice, implementation methods will now be described by way of non-limiting example only, with reference to the accompanying drawings, in which:

[0015] Figures 1A to 1Q These are illustrations of stickers based on some examples of currently available themes.

[0016] Figures 2A to 2E These are schematic diagrams of consumer product packaging based on some examples of currently available topics.

[0017] Figures 3A to 3C An example based on the currently disclosed topic is shown on day zero ( Figure 3A ), 13 days later ( Figure 3B ) and after 20 days ( Figure 3C (A sticker that adheres to bread packaging.)

[0018] Figures 4A to 4C An example based on the currently disclosed topic is shown on day zero ( Figure 4A ), 10 days later ( Figure 4B) and after 25 days ( Figure 4C (A sticker that is attached to the cake packaging.)

[0019] Figure 5 This is a bar graph showing the mold count results from a test conducted at 13°C and 75% RH on a sample identified as Str.1.

[0020] Figures 6A to 6D The images were taken 7 days after the initial follow-up, based on representative images from the Str.1 mold counting plate. These images showed differences in the microbiome of the treatment group compared to the control group, as well as a reduction in mold colonies, including: total control count ( Figure 6A ), control yeast and mold ( Figure 6B ), total number of experiments ( Figure 6C ) and experimental yeasts and molds ( Figure 6D ).

[0021] Figures 7A to 7D These are representative images of packaged strawberries from experiment Str.2 after 7 days of storage at 13°C and 75% RH, including: the control group at T0 ( Figure 7A ) and the control group of T7 ( Figure 7B ) and the experimental group T0 ( Figure 7C ) and the experimental group at T7 ( Figure 7D ).

[0022] Figures 8A to 8D These are representative images of packaged strawberries from experiment Str.3 after storage at ambient room temperature, including: the control group at T0 ( Figure 8A ) and the T3 control group ( Figure 8B ) and the experimental group T0 ( Figure 8C The experimental groups of T3 and T3 ( Figure 8D ).

[0023] Figures 9A to 9D These are representative images of packaged strawberries from the Str.5A test after being stored at 4°C / 75%-90% RH for 5 days and then at 13°C / 75% RH for 4 days, including: the control group at T0 ( Figure 9A ) and the T9 control group ( Figure 9B ) and the experimental group T0 ( Figure 9C ) and the T9 experimental group ( Figure 9D ).

[0024] Figures 10A to 10D These are representative images of packaged strawberries from the Str.5B test after being stored at 4°C / 75%-90% RH for 5 days and then at 13°C / 75% RH for 7 days, including: the control group at T0 ( Figure 10A ) and the control group of T12 ( Figure 10B ) and the experimental group T0 ( Figure 10C) and the experimental group of T12 ( Figure 10D ).

[0025] Figures 11A to 11D These are representative images of packaged strawberries from the Str.5C test after being stored at 4°C / 75%-90% RH for 5 days and then at 13°C / 75% RH for 8 days, including: the control group at T0 ( Figure 11A ) and the T13 control group ( Figure 11B ) and the experimental group T0 ( Figure 11C ) and the experimental group of T13 ( Figure 11D ).

[0026] Figure 12 This is a bar graph showing the mold count results from a test conducted at 13°C and 75% RH, which identified the mold as Tmt.1.

[0027] Figures 13A to 13D Representative images from the mold counting chamber of experiment Tmt.1 were obtained after a 6-day follow-up. These images show the differences in the microbiome of the treatment group compared to the control, as well as the reduction in mold colonies, including: total control count ( Figure 9A ), control yeast and mold ( Figure 9B ), total number of experiments ( Figure 9C ) and experimental yeasts and molds ( Figure 9D ).

[0028] Figure 14 This is a bar graph showing the mold count results from a test conducted at 13°C and 75% RH, identified as Tmt.3.

[0029] Figures 15A to 15D Representative images from the mold counting chamber of experiment Tmt.3 were obtained after a 7-day follow-up. These images show the differences in the microbiome of the treatment group compared to the control, as well as the reduction in mold colonies, including: total control count ( Figure 15A ), control yeast and mold ( Figure 15B ), total number of experiments ( Figure 15C ) and experimental yeasts and molds ( Figure 15D ).

[0030] Figures 16A to 16B The image includes a mango, and the results of a shelf-life study on sticker-protected mangoes were shown in duplicate during follow-up periods of 5, 7, and 10 days, including a control group ( Figure 16A ), compared to the experimental group ( Figure 16B ). Detailed Implementation

[0031] The currently disclosed subject matter relates to a preservation sticker used to extend the shelf life of consumer products (such as fresh food products and agricultural products) by stimulating the growth of selected bacteria within the packaging containing the items, without using live bacteria added externally to the consumer products, thereby eliminating the difficulties and costs associated with the use of live bacteria in industrial plants.

[0032] In the context of this disclosure, food preservation stickers or preservation labels or stickers refer to a unit form used to preserve consumer products from spoilage due to the growth of pathogens and unwanted microorganisms on the article. The article can be any edible material (including industrially produced food, fresh agricultural products, crops, etc.) as well as inedible articles with a tendency to spoil / rot (such as cosmetics, household care products, and pharmaceuticals).

[0033] All of the above are collectively referred to as "items" in this article.

[0034] The preservation stickers are designed to selectively support the growth of human-friendly bacteria that are naturally present in the environment (in the air, i.e., airborne bacteria; in the soil; or on the item itself). Unbound by theory, it is believed that the selective growth of such bacteria on the stickers, and their internal diffusion on or around the item (through water vapor or biofilm formation), allows for preservation by competing with putrefactive or pathogenic bacteria and secreting antimicrobial bacteriocins.

[0035] In some examples, food preservation stickers can be used to prevent the growth of pathogenic and / or spoilage microorganisms in packaged items, thereby increasing the shelf life of the items.

[0036] In some examples, the preservation stickers disclosed herein extend the shelf life of an item by at least 10%, sometimes at least 20%, sometimes at least 30%, sometimes at least 50%, sometimes at least 60%, sometimes at least 70%, sometimes at least 80%, sometimes at least 90%, and sometimes even 100%, compared to the shelf life of the same item packaged under the same conditions without preservation stickers.

[0037] Therefore, the currently disclosed subject provides a sticker comprising at least one adhesive component containing a carrier composition for containing a nutrient composition; wherein the carrier composition contains a food-grade adhesive, and the nutrient composition contains at least one compound suitable for supporting the selective growth of one or more human-safe bacteria.

[0038] In the context of this disclosure, when referring to "sticker," it should be understood to mean a material having an adhesive layer configured to adhere to a specified surface. In some examples, the sticker is in the form of a roll. In some examples, the sticker is in the form of a single pre-cut sheet. In some examples, the pre-cut sheet has a geometry, such as rectangular, triangular, polygonal, circular, elliptical, etc.

[0039] In the context of this disclosure, when references are made to “adhesive layer,” “adhesive composition,” “adhesive assembly,” or “adhesive,” they are to be understood as referring to a compound or composition configured to form an adhesive when adhered to a specified surface.

[0040] In some examples, the adhesive components of the currently disclosed subject matter are configured to support the selective growth of one or more human-safe bacteria and are substantially free of externally added microorganisms. For example, the adhesive component comprises a carrier composition having a food-grade binder that contains a nutrient composition. The nutrient composition comprises at least one compound or nutrient combination for supporting the selective growth of human-safe bacteria while inhibiting or preventing the growth of unwanted microorganisms. In other words, in the presence of selective nutrients, primarily only the desired human-safe bacteria grow effectively, and the proliferation of human-safe bacteria is sufficient to prevent the unwanted growth of putrefactive microorganisms.

[0041] In the context of this disclosure, when referring to selectively promoting the growth of human-safe bacteria, it should be understood as primarily allowing the growth of only the target bacteria (e.g., bacteria that produce bacteriocins), without detectable amounts of other microorganisms (such as yeast, Enterococcus, etc.) or the growth of other microorganisms less than 100 CFU / g or less than 10 CFU / cm³. 2 .

[0042] In this scenario, with the availability of currently disclosed devices featuring selective nutrient compositions, the detectable amount of selectively grown bacteria will be greater than 10 CFU / g, or at least greater than 10. 2 CFU / g, also known as total viable count or total plate count, is a test that measures the total number of viable microorganisms in a sample. Several official and widely accepted methods exist for total microbial count testing. One of the most common methods is the pour plate method using "Nutrient Agar or Standard Methods Agar," as described in ISO 4833.

[0043] The practice of constructing nutrient compositions that selectively support the growth of specific target microorganisms is known from diagnostic microbiology. For example, but not limited to, Bonnet et al. demonstrated selective culture of target microorganisms [M Bonnet et al., “Bacterial culture through selective and none-selective conditions: the evaluation of culture media in clinical microbiology” New Microbe and New Infect 2020; 34:100622]. Furthermore, as a non-limiting example, MRS media are known to be selective for the growth of lactic acid bacteria. Further exemplary literature includes Nwadiuto O. Nwamaioha and Salam A. Ibrahim, “A selective medium for the enumeration and differentiation of Lactobacillus delbrueckii ssp. bulgaricus” J. Dairy Sci. 101:4953–4961.

[0044] In the context of the currently published subject matter, the term "selective growth" should be understood as referring to the specific promotion of the growth of bacteria / human-safe bacteria that produce the target bacteriocin, while inhibiting the growth of other putrefactive bacteria or pathogens.

[0045] In the context of this disclosure, "human-safe bacteria" means any bacteria that is recognized as safe for human consumption and has a long history of use in conventional foods, which are permitted by global regulations for human consumption.

[0046] In some examples, human-safe bacteria include lactic acid bacteria (LAB). These bacteria are known to inhibit the growth of unwanted microorganisms, particularly putrefactive and pathogenic microorganisms. This inhibitory activity results from the fact that metabolites secreted by these LABs act as antimicrobial compounds. These compounds include organic acids, diacetone, hydrogen peroxide, and bacteriocins.

[0047] The stickers disclosed in this article can support the growth of various LABs. Examples of LABs include Lactobacillus Plantarum, Lactobacillus Lactis, and Lactobacillus Brevis.

[0048] In some other examples, the stickers disclosed herein can support the growth of bacteria other than LAB, such as species of the genus Bacillus. These bacteria include, but are not limited to, Bacillus subtilis, Bacillus pumilis, Bacillus safensis, Bacillus thurgiensis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus coagulans, and Bacillus brevis.

[0049] In some examples of currently disclosed topics, human-safe bacteria include probiotics. The nutrient composition contained within the carrier device comprises different types of substances, each acting as a source of different nutrients required for the growth of human-safe (e.g., bacteriocin-producing) bacteria.

[0050] Unbound by theory, selective growth is achieved by providing specific nutritional requirements for the desired bacteria (i.e., the nutritional compositions disclosed herein) and creating conditions that inhibit the growth of other undesirable microorganisms. Such conditions include, for example, any or a combination of preservatives, pH controllers, oxygen management, and temperature control.

[0051] In some examples, selective growth can be supported or achieved by any or a combination of the following: controlling oxygen levels, for example, monitoring or specifically designing the permeability of the packaging containing the article; controlling storage temperature; controlling pH using a specifically chosen pH adjuster, etc.

[0052] The ratio between the different components of the sticker can vary depending on the item to be preserved, the storage conditions (temperature, humidity, etc.), and the type of human-safe microorganisms (to which stimulation is desired).

[0053] In the context of this disclosure, when referring to “substantially free of externally added microorganisms”, it should be understood that no microorganisms were added to the stickers, and the microorganisms found on the stickers are only those that grew during the shelf life.

[0054] In the context of this disclosure, when referring to spoilage microorganisms, it should be understood to encompass microorganisms that cause adverse changes to the quality of articles (causing articles (such as food) to spoil and produce unpleasant odors, tastes, and textures, for example, making fruits and vegetables mushy or sticky, or giving meat an unpleasant odor) but are not toxic to food. Spoilage microorganisms typically metabolize gases, acids, sulfur compounds, and nitrogen compounds that affect the sensory properties of articles (especially when food is involved).

[0055] When referring to pathogenic microorganisms, it should be understood to encompass microorganisms that are harmful to food or that produce harmful or deadly toxins when ingested. Some non-limiting examples of pathogenic microorganisms include Campylobacter, Salmonella, Escherichia coli (E. coli), Clostridium perfringens, Bacillus cereus, Listeria monocytogenes, Shigella spp., Staphylococcus aureus, Streptococcus, and Vibrio bacteria.

[0056] In some examples, the carrier composition contains a food-grade desiccant.

[0057] In the context of this invention, the terms "hygroscopic material" or "hygroscopic agent" refer to materials that readily absorb moisture from the atmosphere. It should be noted that the adhesive component can be in solid, semi-solid, or high-viscosity form. When exposed to the ambient atmosphere, the adhesive composition absorbs a significant amount of moisture. Without being bound by theory, it is currently believed that moisture absorption activates the adhesive composition, thereby enabling the composition to support the growth of human-friendly bacteria.

[0058] In some examples, the hygroscopic material is any one or a combination of polyols, polysaccharides, salts, and silicon compounds.

[0059] Non-limiting examples of hygroscopic polyols include glycerol, sorbitol, propylene glycol, lactitol, xylitol, erythritol, isomaltitol, mannitol, maltitol, and any combination thereof.

[0060] In some examples, hygroscopic polysaccharides include, but are not limited to, maltodextrin, dextrin, polydextrose, and any combination thereof.

[0061] In some examples, hygroscopic salts include, but are not limited to, potassium gluconate, calcium gluconate, sodium gluconate, potassium acetate, sodium acetate, calcium acetate, sodium lactate, sodium citrate, potassium citrate, calcium citrate, sodium maleate, potassium maleate, calcium maleate, potassium aluminum silicate, aluminum silicate, calcium glutamate, monoammonium glutamate, magnesium diglutamate, sodium acetate, sodium chloride, calcium chloride, and any combination thereof.

[0062] In some examples, hygroscopic silicon compounds include silicon dioxide, calcium silicate, sodium aluminosilicate, and magnesium silicate.

[0063] In some examples, the hygroscopic compound includes at least sodium chloride.

[0064] In some examples, the hygroscopic compound includes at least glycerol.

[0065] In some examples, the hygroscopic compounds include a combination of sodium chloride and glycerol.

[0066] In some examples, the amount of the hygroscopic compound (whether a single hygroscopic compound or a combination of several such additives) is in the range of 1% to 15% of the total weight of the adhesive component, sometimes between 1.5% and 3%, sometimes between 2% and 5%, sometimes between 4% and 8%, sometimes between 6% and 12%, sometimes between 10% and 15%, or any other range in the range of 1% to 15%.

[0067] In some examples, the food-grade binder of the carrier composition is selected from the group consisting of polysaccharides, protein-like materials, waxes, or combinations thereof.

[0068] It is worth noting that, in the context of this disclosure, the term "food grade" means any material that is acceptable and approved for human consumption.

[0069] In some examples, the polysaccharide is selected from the group consisting of: starch, modified starch, alginate, pectin, carrageenan, locust bean gum, guar gum, xanthan gum, dextrin, agarose, agar, amylopectin, cellulose, sodium carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, sodium alginate, potassium alginate, ammonium alginate, calcium alginate, 1,2-propanediol alginate, tragacanth gum, chitosan, gum arabic, ark arabic gum, tara gum, gellan gum, and any combination thereof.

[0070] In some examples, the protein or protein-like material is selected from the group consisting of: gelatin, soybean hydrolysate, casein, whey protein, pea protein, mung bean protein, and soybean protein.

[0071] In some examples, the protein or protein-like material is selected from the group consisting of: animal-derived proteins, plant-derived proteins, insect-derived proteins, algal-derived proteins, and any combination thereof.

[0072] In some examples, the protein or protein-like material is selected from the group consisting of: rice, corn, wheat, barley, oats, sorghum, buckwheat, amaranth, quinoa, peas, chickpeas, lentils, lupins, flaxseed, soybeans, peanuts, sunflower seeds, cotton, sesame seeds, chia seeds, pumpkin, almonds, chestnuts, pecans, walnuts, gelatin, casein, whey protein, beef protein, bovine protein, and any combination thereof.

[0073] In some examples, the wax is selected from the group consisting of: beeswax, carnauba wax, candelilla wax, soy wax, shellac, microcrystalline wax, and any combination thereof.

[0074] In some examples, the amount of adhesive is about 40% to about 70% of the total weight of the adhesive component, sometimes about 50% to about 65%, sometimes about 55% to about 65%, sometimes about 65% to about 70%, or any other range within the range of about 50% to about 70%.

[0075] In some examples, the food-grade binder includes dextrin. The amount of dextrin is from about 40% to about 70% by weight, preferably from about 55% to about 65% by weight, of the total weight of the binder components.

[0076] In some examples, the food-grade desiccant includes at least one salt. The salt may be sodium chloride, and its amount is from about 1% to 15% by weight of the total weight of the adhesive component, preferably from about 2% to 10% by weight of the total weight of the adhesive component.

[0077] In some examples, the carrier composition may contain different types of substances (nutrient compositions), each acting as a source of different nutrients required for the growth of human-safe bacteria. The nutrient compositions are blended into the carrier composition.

[0078] In some examples, the nutritional composition comprises a combination of compounds selected from the group consisting of nitrogenous compounds, carbohydrates, inorganic minerals, inorganic salts, fatty acids, and vitamins.

[0079] In some examples, at least one nitrogen-containing compound is selected from the group consisting of at least one amino acid, at least one peptide, at least one polypeptide, and at least one protein hydrolysate. These can be obtained from a variety of sources.

[0080] In some examples, the source of the nitrogenous compound is animal-derived, such as beef extract and / or casein hydrolysate.

[0081] In some examples, the source of nitrogenous compounds is microbial extracts, such as yeast extracts.

[0082] In some examples, the source of nitrogenous compounds is plant extracts, such as legume extracts. In this context, legumes can be any type of bean, such as, but not limited to, peas, chickpeas, potatoes, soybeans, kidney beans, etc.

[0083] In some examples, nitrogen-containing compounds comprise a combination of one or more extracts and one or more hydrolysates from different sources.

[0084] In some examples, the nitrogen-containing compound contains at least protein hydrolysate, sometimes peptone, and sometimes preferably soybean peptone.

[0085] In some examples, the nitrogen source contains at least yeast extract.

[0086] In some examples, the nitrogen source contains at least animal extracts.

[0087] In some examples, the nitrogen source contains at least plant extracts.

[0088] In some examples, the nitrogen source contains at least protein hydrolysate and yeast extract.

[0089] In some examples, the nitrogen source comprises a combination of protein hydrolysates, yeast extracts, and at least one of animal or plant extracts.

[0090] In some examples, the amount of nitrogenous compound / source (whether a single source or a combination of sources) is 0.1% to 20% of the total weight of the nutrient composition, sometimes 0.5% to 10%, sometimes 0.5% to 5%, sometimes 1% to 5%, sometimes 1% to 3%, sometimes 0.5% to 3%, sometimes 0.1% to 3%, or any other range within the range of 0.1% to 20%.

[0091] In some examples, the nutrient composition contains one or more carbon-containing compounds that act as a carbon source for the growth of bacteria that are required to be safe for humans.

[0092] In some examples, the carbon-containing compound is one or more carbohydrates.

[0093] In some examples, carbohydrates are selected from the group consisting of monosaccharides, disaccharides, and oligosaccharides.

[0094] In some examples, carbohydrates are selected from the group consisting of: mannitol, arabinose, xylose, glucose, galactose, maltose, raffinose, sucrose, lactose, fructooligosaccharides (FOS), and sorbitol.

[0095] In some examples, the carbon-containing compound is a sugar or contains sugars, which in particular include any one or a combination of the following: monosaccharides, such as glucose, fructose, galactose, xylose, arabinose; disaccharides, such as sucrose, lactose, maltose, isomaltulose, trehalose, trealulose, and trehalulose; and oligosaccharides, that is, those oligosaccharides that typically contain 3 to 10 monosaccharides, such as raffinose (trisaccharide), fructooligosaccharides (FOS), galactooligosaccharides (GOS), oligodextrose, isomaltulose, maltotriose, etc.

[0096] Other carbohydrates can include sugar alcohols, such as mannitol and sorbitol.

[0097] In some examples, the carbon-containing compound is at least mannitol.

[0098] In some examples, the carbohydrate / carbon-containing compounds contain at least glucose or at least a combination of glucose and FOS.

[0099] In some examples, the amount of carbohydrates (whether from a single source or a combination of carbohydrates) ranges from 0.5% to 5% of the total weight of the nutritional composition, sometimes between 1% and 5%, sometimes between 0.5% and 4%, sometimes between 0.5% and 2.5%, sometimes between 1% and 4%, sometimes between 1% and 3%, sometimes between 1% and 2.5%, or any other range between 0.5% and 5%.

[0100] In some examples, the nutritional composition contains at least one inorganic mineral and / or at least one inorganic salt.

[0101] In some examples, at least one inorganic mineral and / or at least one inorganic salt includes any or a combination of phosphates, sulfates, potassium, calcium, zinc, magnesium, manganese, and iron.

[0102] In some examples, the source of such minerals / salts is yeast extract. It is worth noting that, in the context of this disclosure, yeast extract can be considered to provide several types of nutrients, including minerals, vitamins, and / or digestible nucleic acids.

[0103] In some examples, the nutritional composition contains at least a manganese salt, such as manganese sulfate.

[0104] In some examples, the nutritional composition contains at least a magnesium salt, such as magnesium sulfate.

[0105] In some examples, the nutritional composition contains at least a sodium salt, such as sodium acetate.

[0106] In some examples, the nutritional composition contains at least a potassium salt, such as dipotassium hydrogen phosphate.

[0107] In some examples, the nutritional composition contains at least an ammonium salt, such as triammonium citrate.

[0108] In some examples, the nutritional composition contains at least a combination of manganese and magnesium salts.

[0109] In some examples, the amount of inorganic minerals / inorganic salts (whether a single inorganic component or a combination of inorganic substances) is in the range of 0.005% to 5% of the total weight of the nutrient composition, sometimes between 0.01%-2% and 0.02%-1%, sometimes between 0.005% and 2%, sometimes between 0.05% and 1%, sometimes between 0.01% and 3%, or any other range in the range of 0.005% to 5%.

[0110] In some examples, the nutritional composition contains at least one fatty acid.

[0111] In the context of this invention, the term "fatty acid" refers to simple fatty acids, i.e., those fatty acids having a carboxylic acid head group and a saturated or unsaturated aliphatic tail, and also to complex fatty acids in which the head group is replaced by a macromolecule (such as a polyoxyethylene group). Such fatty acids can also be used as surfactants and / or emulsifiers in nutritional compositions.

[0112] Aliphatic chains can contain any number of carbon atoms, including short-chain fatty acids with up to 5 carbon atoms at the tail, medium-chain fatty acids with 6-12 carbon atoms at the tail, long-chain fatty acids with 13-21 carbon atoms at the tail, and very long-chain fatty acids with 22 or more carbon atoms at the aliphatic tail.

[0113] In some examples, at least one fatty acid is selected from the group consisting of: caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, behenic acid, oleic acid, and linoleic acid.

[0114] In some examples, the nutritional composition contains at least one vitamin. Vitamins are known to be essential for the normal function and metabolism of organisms, and are therefore required even in very low amounts.

[0115] In some examples, at least one vitamin is any or a combination of nicotinic acid (vitamin B13), calcium pantothenate (a calcium salt of vitamin B5), pyridoxine (vitamin B6), and vitamin B12. In some examples, the nutritional composition contains at least calcium pantothenate.

[0116] In some examples, the vitamin component contains at least nicotinic acid.

[0117] In some examples, the vitamin contains a combination of nicotinic acid and calcium pantothenate.

[0118] In some examples, the amount of vitamins ranges from 0.0001% to 5%, sometimes from 0.0001% to 3%, sometimes from 0.005% to 5%, sometimes from 0.008% to 2%, and in any other range between 0.0001% and 5%. It is worth noting that vitamins can be obtained from yeast extracts and can also be added externally.

[0119] In some examples, the nutritional composition contains at least one surfactant / emulsifier.

[0120] In some examples, at least one surfactant is selected from the group consisting of: polyoxyethylene (20) sorbitol monolaurate, polyoxyethylene (40) sorbitol monopalmitate, polyoxyethylene (60) sorbitol monostearate, polyoxyethylene (20) sorbitol monooleate and lecithin.

[0121] In some examples, the surfactant / emulsifier components of the nutritional composition include one or a combination of polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80, and lecithin.

[0122] In some other examples, the surfactant / emulsifier component contains at least polysorbate 80.

[0123] In some examples, the amount of surfactant / emulsifier (whether a single surfactant / emulsifier or a combination of several such additives) is in the range of 0.001% to 5% of the total weight of the nutritional composition, sometimes between 0.005% and 3%, sometimes between 0.01% and 2%, sometimes between 0.01% and 3%, sometimes between 0.05% and 3%, sometimes between 0.05% and 2%, sometimes between 0.01% and 1%, sometimes between 0.05% and 1%, sometimes between 0.06% and 0.11%, or any other range in the range of 0.001% to 5%.

[0124] In some examples, the surfactant / emulsifier is or contains fatty acid esters or any other surfactant / emulsifier acceptable under food regulations (E-number).

[0125] According to some examples, the nutrient composition also contains a buffer. The purpose of the buffer is, in particular, to maintain the pH of the nutrient composition within a pH range that supports the growth of bacteria required to produce bacteriocins. In some examples, the buffer includes any or a combination of phosphoric acid, citric acid, lactic acid, and glycine. In some examples, the nutrient composition has a pH in the range of about 5.5 to about 7.

[0126] In some examples, the buffer contains at least citric acid.

[0127] The amount of buffer will depend on the type of reagent used. In some examples, the amount is chosen to provide a pH in the range of 4.0 to 7.0, sometimes between 4.0 and 6.0, sometimes between 4.2 and 6.0, sometimes between 4.7 and 5.8, and sometimes between 4.7 and 5.8.

[0128] In yet another or additional examples, the nutrient composition contains one or more preservatives (growth inhibitors). Non-limiting examples of preservatives include potassium sorbate, sodium benzoate, sodium chloride, sodium lactate, bacteriocins, long-chain polyphosphates, ammonium citrate, sodium acetate, and maltol.

[0129] In one example, the preservative is potassium sorbate.

[0130] In some examples, the amount of preservative added to the nutrient composition ranges from 0.01% to 0.2% of the total weight of the composition, sometimes between 0.05% and 0.1%, sometimes between 0.02% and 0.08%, or any other range between 0.01% and 0.2%. This amount can vary depending on the preservative used.

[0131] In some examples, the nutrient composition also contains inactivated cell extracts of LAB, which can help support the growth of LAB on the sticker. Examples of such inactivated cell extracts may include, but are not limited to, extracts of Lactobacillus acidophilus and Lactobacillus salivarius.

[0132] Nutritional compositions can be prepared by any method known in the art. In some examples, adhesive components are prepared by mixing the components of the nutritional composition with an adhesive and a hygroscopic agent until a homogeneous mixture is formed.

[0133] In some examples, the nutritional composition comprises: a binder material, preferably dextrin, in an amount of about 50% to 70% by weight; a hygroscopic material, preferably sodium chloride and / or propylene glycol, in an amount of 6% to 12% by weight; a nitrogenous compound, preferably soybean peptone, in an amount of about 1% to about 5% by weight; carbohydrates, preferably mannitol, in an amount of about 0.5% to about 4% by weight; minerals and / or salts, preferably sodium acetate, potassium dihydrogen phosphate, trisodium citrate, magnesium sulfate and / or manganese sulfate, in a total amount of about 0.05% to 3% by weight; optionally a preservative additive, preferably potassium sorbate, in an amount of about 0.01% to 0.2% by weight; and a buffer, preferably citric acid, in an amount that provides a pH of 4 to 7.

[0134] The currently available theme provides several specific implementations of stickers based on various examples, some of which are referenced below. Figures 1A to 1QThe sticker 10 shown in the illustration is described below.

[0135] Figure 1A A sticker 10 including an adhesive component 11 is schematically shown. The adhesive component 11 comprises a carrier composition containing a food-grade adhesive and a nutrient composition suitable for supporting the selective growth of one or more human-safe bacteria. In fact, according to various examples, the adhesive component 11 may include all or some of the features of any combination of the adhesive components described herein. The adhesive component 11 has a first adhesive component side 11A and an opposing second adhesive component side 11B. According to various examples as described later herein, the adhesive component 11 is configured to adhere to packaging containing an article to be preserved via either the first adhesive component side 11A or the second adhesive component side 11B.

[0136] Figure 1B A sticker 10 is schematically shown, comprising a (first) backing material 12 disposed on a first side 11A of the adhesive assembly 11. In the context of this disclosure, when referred to as "backing material," it should be understood to encompass a variety of materials selected from paper (cellulose blends), fabrics, porous metal foils, silicon, synthetic polymers, naturally occurring polymers, and combinations thereof, and which are permeable to moisture and microorganisms. Moisture from the article can permeate through the backing material and activate the adhesive assembly to stimulate the growth of human-safe bacteria.

[0137] The first backing material 12 has a first backing material first side 12A facing and adhering to the adhesive assembly 11 and an opposite first backing material second side 12B.

[0138] In some examples, the adhesive components are evenly distributed across the entire surface of the backing material, thus providing 100% coverage. In other examples, the adhesive components are distributed in a pattern, thus providing a smaller percentage of coverage. Sometimes the coverage is about 95% to about 20%, sometimes about 90% to about 30%, sometimes about 80% to about 40%, sometimes about 70% to about 50%, and sometimes about 50% to about 20%.

[0139] Figure 1C The sticker 10 is schematically shown. The sticker includes an adhesive assembly 11, a first backing material 12, and a first fastening element 13 disposed on a second side 12B of the first backing material.

[0140] In the context of this disclosure, when referring to "fixing element," it should be understood to encompass elements configured to adhere to a specified surface and thus secure a sticker thereon. In some examples, the fixing element is an inert adhesive (i.e., encompassing the properties of an adhesive and free of nutritional compositions or food-grade adhesives).

[0141] In some examples, the first fastening elements are evenly distributed across the entire surface of the backing material, thus providing 100% coverage. In other examples, the first fastening elements are distributed in a pattern to provide a smaller percentage of coverage, depending on the required adhesion strength when the sticker is adhered to packaging containing the item.

[0142] The first fixing element 13 has a first fixing element first side 13A facing and adhering to the first backing material 12 and an opposing first fixing element second side 13B configured for adhering to a consumer product (packaging containing articles), thereby securing the sticker 10 thereto. It should be understood herein that the sticker 10 may be adhered to the packaging containing articles via the fixing element 13 or via the adhesive assembly 11, depending on the desired exposure of the adhesive assembly 11 to water vapor from the articles.

[0143] Figure 1D The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fastening element 13, and a removable first fastening element liner 14 disposed on a second side 13B of the first fastening element.

[0144] In the context of this disclosure, when referring to “removable lining”, it should be understood to encompass a removable layer configured to protect the adhesive properties of the underlying adhesive material and to be easily removed before the application of a sticker.

[0145] In the example, when the sticker 10 is to be applied to the packaging via the first fastening element 13, the removable first fastening element liner 14 is removed and the sticker 10 is applied to the packaging.

[0146] Figure 1E The sticker 10 is schematically shown, and the sticker includes an adhesive component 11, such as... Figure 1A The example shown illustrates a removable adhesive assembly liner 15 disposed on the second side 11B of the adhesive assembly. In the context of this disclosure, when referring to "removable liner," it should be understood to encompass a removable layer configured to protect the adhesive properties of the underlying adhesive material and easily removable before application of the sticker. In the example, when the sticker 10 is to be applied to the packaging via the adhesive assembly 11, the removable adhesive assembly liner 15 is removed and the sticker is applied to the packaging. In some examples, the removable adhesive assembly liner may be disposed on both sides of the adhesive assembly.

[0147] Figure 1F The sticker 10 is schematically shown, which includes an adhesive component 11 and a first backing material 12 (such as...). Figure 1B(as shown in the example) and a removable adhesive assembly liner 15 disposed on the second side 11B of the adhesive assembly.

[0148] Figure 1G The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, and a first fixing element 13 (such as...). Figure 1C (as shown in the example) and a removable adhesive assembly liner 15 disposed on the second side 11B of the adhesive assembly.

[0149] Figure 1H The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fastening element 13, and a removable first fastening element liner 14 (e.g., Figure 1D (as shown in the example) and a removable adhesive assembly liner 15 disposed on the second side 11B of the adhesive assembly.

[0150] Figure 1I The sticker 10 is schematically shown, which includes an adhesive component 11 and a first backing material 12 (such as...). Figure 1B (as shown in the example) and a second backing material 16 disposed on the second side 11B of the adhesive assembly.

[0151] In the context of this disclosure, when referring to "backing material," it should be understood to encompass a variety of materials selected from paper (cellulose blends), fabrics, porous metal foils, silicon, synthetic polymers, naturally occurring polymers, and combinations thereof, and which are permeable to moisture and microorganisms. Moisture from the article can permeate through the backing material and activate the adhesive components to stimulate the growth of human-safe bacteria. In some examples, the second backing material is the same as the first backing material. In some examples, the second backing material is different from the first backing material.

[0152] The second backing material 16 has a first side 16A of the second backing material facing and adhering to the adhesive assembly 11 and a second side 16B of the opposite second backing material.

[0153] In some examples, the adhesive components are evenly distributed across the entire surface of the backing material, thus providing 100% coverage. In other examples, the adhesive components are distributed in a pattern, thus providing a smaller percentage of coverage. Sometimes the coverage is about 95% to about 20%, sometimes about 90% to about 30%, sometimes about 80% to about 40%, sometimes about 70% to about 50%, and sometimes about 50% to about 20%.

[0154] Figure 1J The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, and a first fixing element 13 (such as...). Figure 1C(as shown in the example) and a second backing material 16 disposed on the second side 11B of the adhesive assembly.

[0155] Figure 1K The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fastening element 13, and a removable first fastening element liner 14 (e.g., Figure 1D (as shown in the example) and a second backing material 16 disposed on the second side 11B of the adhesive assembly.

[0156] Figure 1L The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, and a second backing material 16 (e.g., ...). Figure 1I (as shown in the example) and a second fastening element 17 disposed on the second side 16B of the second backing material.

[0157] In the context of this disclosure, when referring to "fixing element," it should be understood to encompass elements configured to adhere to a designated surface (whether it be an item to be preserved or packaging containing the item) and thus secure the sticker thereto. In some examples, the fixing element is an inert adhesive (i.e., encompassing the properties of an adhesive and free of nutritional compositions or food-grade adhesives).

[0158] In some examples, the second fastening elements are evenly distributed across the entire surface of the backing material, thus providing 100% coverage. In other examples, the second fastening elements are distributed in a pattern to provide a smaller percentage of coverage, depending on the required adhesion strength when the sticker is adhered to packaging containing the item.

[0159] The second fixing element 17 has a second fixing element first side 17A facing and adhering to the second backing material 16 and an opposing second fixing element second side 17B configured to adhere to a consumer product (packaging containing articles), thereby fixing the sticker 10 thereon.

[0160] Figure 1M The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fixing element 13, and a second backing material 16 (e.g., ...). Figure 1J (as shown in the example) and a second fastening element 17 disposed on the second side 16B of the second backing material. It should be understood herein that the sticker 10 may be adhered to the packaging containing the article via the first fastening element 13 or via the second fastening element 17.

[0161] Figure 1N The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fastening element 13, a removable first fastening element liner 14, and a second backing material 16 (e.g., ...). Figure 1K (as shown in the example) and a second fastening element 17 disposed on the second side 16B of the second backing material.

[0162] Figure 10 The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a second backing material 16, and a second fixing element 17 (e.g., ...). Figure 1L (as shown in the example) and a removable second fastening element liner 18 disposed on the second side 17B of the second fastening element.

[0163] In the context of this disclosure, when referring to “removable lining”, it should be understood to encompass a removable layer configured to protect the adhesive properties of the underlying adhesive material and to be easily removed before the application of a sticker.

[0164] In some examples, when the sticker 10 is to be applied to the packaging via the second fastening element 17, the removable second fastening element liner 18 is removed and the sticker 10 is applied to the packaging.

[0165] Figure 1P The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fixing element 13, a second backing material 16, and a second fixing element 17 (e.g., ...). Figure 1M (as shown in the example) and a removable second fastening element liner 18 disposed on the second side 17B of the second fastening element.

[0166] Figure 1Q The sticker 10 is schematically shown, which includes an adhesive assembly 11, a first backing material 12, a first fastening element 13, a removable first fastening element liner 14, a second backing material 16, and a second fastening element 17 (e.g., Figure 1N (as shown in the example) and a removable second fastening element liner 18 disposed on the second side 17B of the second fastening element.

[0167] In some examples that include both a removable first fastening element liner 14 and a second fastening element liner 18, the two liners may be the same as or different from each other.

[0168] In some examples, which include either the adhesive component liner 15 or the removable first fastening element liner 14 and the second fastening element liner 18, the two liners may be the same as or different from each other.

[0169] It should be understood herein that the sticker 10 according to any of the examples above can be placed near the item to be preserved, for example, by adhering the sticker to the item or the packaging containing the item, such that the moisture of the item can activate the nutrient composition and microorganisms (bacteria) can grow within the packaging. For example, according to various examples, the sticker can be applied to the inside or outside of the packaging at moisture channels formed between the internal volume of the packaging and the adhesive assembly to activate the nutrient composition by water vapor (moisture from the item), some of which are referred to below. Figures 2A to 2E The description is based on a schematic illustration of consumer product packaging. Therefore, the subject matter disclosed herein relates to consumer product packaging comprising packaging elements for containing a consumer product to be preserved and a sticker adhered to said packaging element according to any of the examples described above.

[0170] Figure 2A A consumer product package 100 is shown, which includes a packaging element 101 for containing a consumer product G to be kept fresh.

[0171] In the context of this disclosure, when referring to "packaging element," it should be understood to encompass sleeves, bags, pouches, etc., configured to contain consumer goods. The sticker 10 is adhered to the inside 102 of the packaging element 101 via the adhesive assembly 11. In some examples, the sticker 10 may be as follows: Figure 1B The sticker shown.

[0172] In some examples, sticker 10 can be like this Figure 1F The sticker shown is in which the adhesive component liner is removed before being applied to the packaging element 101.

[0173] Moisture from article G can penetrate through the first backing material 12 and activate the nutrient composition within the adhesive assembly 11, thereby inducing the growth of human-safe bacteria within the packaging 100.

[0174] Figure 2B A consumer product package 200 is shown, comprising a packaging element 201 for containing a consumer product G to be preserved. The packaging element 201 includes a moisture channel 203 between its internal volume and its external surface. A sticker 10 is adhered to the external surface 205 of the packaging element 201 via an adhesive assembly 11.

[0175] In some examples, sticker 10 can be like this Figure 1B The sticker shown.

[0176] In some examples, sticker 10 can be like this Figure 1FThe sticker shown has its adhesive component liner removed before being applied to the packaging element 201. Moisture from the article G can flow through the moisture channel 203 and activate the nutrient composition within the adhesive component 11, thereby inducing the growth of human-safe bacteria within the packaging 200 via the moisture channel 203.

[0177] Figure 2C A consumer product package 100 is shown, which includes a packaging element 101 for containing a consumer product G to be kept fresh. A sticker 10 is adhered to the inside 102 of the packaging element 101 via a first fixing element 13.

[0178] In some examples, sticker 10 can be like this Figure 1J The sticker shown.

[0179] In some examples, sticker 10 can be like this Figure 1K The sticker shown has its removable first fixing element liner removed before being applied to the packaging element 101. Moisture from the article G can penetrate through the second backing material 16 and activate the nutrient composition within the adhesive assembly 11, thereby initiating the growth of human-safe bacteria within the packaging 100.

[0180] Figure 2D A consumer product package 200 is shown, which includes a packaging element 201 for containing a consumer product G to be preserved. The packaging element 201 includes a moisture channel 203 between its internal volume and its external surface. A sticker 10 is adhered to the external surface 205 of the packaging element 201 via a first fixing element 13.

[0181] In some examples, sticker 10 can be like this Figure 1J The sticker shown.

[0182] In some examples, sticker 10 can be like this Figure 1K The sticker shown is wherein its removable first retaining element liner is removed before being applied to the packaging element 201. Moisture from the article G can flow through the moisture channel 203 and penetrate through the first retaining element 13 and the first backing material 12, activating the nutrient composition within the adhesive assembly 11, thereby inducing the growth of human-safe bacteria within the packaging 200 via the moisture channel 203.

[0183] Figure 2E A consumer product package 100 is shown, which includes a packaging element 101 for containing a consumer product G to be kept fresh. A sticker 10 is adhered to the inside 102 of the packaging element 101 via a first fixing element 13.

[0184] In some examples, sticker 10 can be like this Figure 1C The sticker shown.

[0185] In some examples, sticker 10 can be like this Figure 1D The sticker shown is wherein its removable first fixing element liner is removed before being applied to the packaging element 101.

[0186] In some examples, sticker 10 can be like this Figure 1G The sticker shown is wherein its adhesive component liner is removed before being applied to the packaging element 101. In some examples, sticker 10 may be as follows: Figure 1H The sticker shown has its removable first fixing element liner and adhesive component liner removed before being applied to the packaging element 101. The adhesive component 11 is directly and completely exposed to moisture from the article G to activate the nutrient composition, thereby initiating the growth of human-safe bacteria within the packaging 100.

[0187] In some examples, stickers are adhered to items to be preserved, where, for example, the adhesive component liner is removed before being applied to the item, and the adhesive component is directly and completely exposed to moisture from the item, thereby activating the nutrient composition and inducing the growth of human-safe bacteria near the item.

[0188] In some examples, the items to be preserved include food.

[0189] In some examples, the food to be preserved is meat products, including seafood meat, red meat, poultry meat, and vegan alternatives to meat.

[0190] In some examples, the food to be preserved is harvested crops, such as fruits, vegetables, seeds and grains, as well as fruits and vegetables that have already been peeled.

[0191] In some examples, the food to be preserved is dairy products or vegan alternatives to dairy products.

[0192] In some examples, the food to be preserved is ready-to-cook or ready-to-eat food, such as pre-cooked meals, salads, etc.

[0193] In some examples, the food to be preserved is selected from the group consisting of: vegetables, fruits, herbs, grains, baked goods, meat and poultry, fish, dairy products, sauces and condiments.

[0194] In some examples, consumer products have high water activity and are therefore more susceptible to microbial spoilage. Examples of such products include, but are not limited to, fresh produce, food products such as cheese, meat, salads, sauces, cosmetics and toiletries, and household cleaning products.

[0195] In some examples, consumer goods are items that would otherwise need to be stored in the refrigerator. With the presence of food storage stickers, such products can be stored at room temperature. Examples of such products may include, but are not limited to, salad dressings, meat, fish, cheese, fruits and vegetables, and pre-peeled and pre-cut fruits and vegetables.

[0196] In some examples, the item to be preserved / protected is strawberries.

[0197] In some examples, the item to be preserved / protected is cherry tomatoes.

[0198] In some examples, the item to be preserved / protected is an avocado.

[0199] In some examples, the item to be preserved / protected is grapes.

[0200] In some examples, the items include household care products, such as cleaning solutions that are typically preserved with chemicals.

[0201] In some examples, the items include medicines in the form of ointments, gels, creams, lotions, or solutions, which are typically preserved with chemicals.

[0202] It should be understood in this document that all the above descriptions of sticker 10 and packaging 100 and 200 are illustrative and that the actual size, structure and shape of sticker and / or packaging 100 / 200 may be changed without altering their operational characteristics.

[0203] More specifically, to illustrate its respective sides, sticker 10 and its individual layers are shown as thickened, and in the actual product, these layers may be particularly thin so that sticker 10 looks like a regular sticker for pasting onto a designated surface.

[0204] The currently disclosed subject matter further relates to a method for preserving consumer products, which involves adhering a sticker, according to any of the examples described above, to a package containing the consumer product in a manner that allows moisture channels to be formed between the consumer product and an adhesive component.

[0205] Alternatively, a method for preserving consumer products is provided, the method comprising adhering a sticker according to any of the examples described above to the surface of the article in a manner that allows moisture channels to be formed between the consumer product and an adhesive component.

[0206] The currently disclosed subject matter further relates to a method for manufacturing a food safety sticker (preservation sticker), the method comprising mixing a carrier composition comprising a food-grade adhesive with a nutrient composition comprising at least one compound suitable for supporting the selective growth of one or more human-safe bacteria to form an adhesive assembly, and shaping the adhesive assembly. The sticker may be a sticker according to any of the examples described above. Therefore, the method includes, according to an example of manufacturing a sticker, disposing a removable liner and / or backing material onto at least one face or side of the adhesive assembly. For example, in some examples, the method may include disposing a first backing material onto a first side of the adhesive assembly and disposing a removable liner onto an opposing second side of the adhesive assembly. In some examples, the method may include attaching a fastening element to the second side of the first backing material opposite the side having the adhesive assembly, the fastening element carrying the removable liner on the side opposite the backing material.

[0207] The currently disclosed subject matter further relates to a method for manufacturing food safety stickers (preservation stickers), the method comprising providing an adhesive assembly comprising a carrier composition containing a nutrient composition. The carrier composition comprises a food-grade adhesive, and the nutrient composition comprises at least one compound suitable for supporting the selective growth of one or more human-safe bacteria. In some examples, the method may include disposing at least one of a first backing material and a first removable liner to at least one side of the adhesive assembly, and optionally disposing at least one of a second backing material and a second removable liner to an opposing second side of the adhesive assembly. The first and second backing materials may be the same or different, and the first and second removable liners may be the same or different.

[0208] The method further includes attaching at least one fastening element to at least one of a first backing material and a second backing material. In some examples, the fastening element may have a removable lining on the side opposite to at least one of the first backing material and the second backing material.

[0209] In some examples, the method may include setting a removable adhesive component liner on a first side of the adhesive component and setting a backing material on the opposite second side of the adhesive component.

[0210] It should be understood in this document that the adhesive components are as defined in any of the examples described herein.

[0211] Detailed description of the non-restrictive implementation scheme

[0212] Example 1 – Preservation of Products with Backing

[0213] Preparation of food preservation adhesive composition

[0214] Two food preservation adhesive compositions were prepared according to Tables 1 and 2.

[0215] Table 1: Adhesive Composition No. 1

[0216]

[0217]

[0218] Table 2: Adhesive Composition No. 2

[0219]

[0220] The adhesive composition is prepared by mixing all the ingredients and suspending them in reverse osmosis (RO) water preheated to 85°C to form a mixture. The mixture is mixed for 20 to 40 minutes to ensure complete dissolution, and then allowed to cool to room temperature.

[0221] An adhesive composition is applied to a paper backing material using a sticker paper coating machine to provide food preservation stickers.

[0222] It should be noted that the adhesive composition can be manually applied to the paper backing material, for example, using a simple brush, or any of the following methods: flexographic printing press, roller coater, brush, sprayer, or any other method for dispensing liquid-based adhesives.

[0223] Food preservation

[0224] bread :

[0225] The sticker is prepared according to the above process, but the adhesive composition as detailed in Table 3 is fixed onto the paper backing material.

[0226] Table 3: Adhesive Composition No. 3

[0227]

[0228] Then, after 20 days, the preservation effect of adhesive composition No. 3 (attached to the paper backing material) on the bagged bread was tested.

[0229] Specifically, apply adhesive composition #3 to the inside of both bread bags, facing the top of the breadsticks. Then reseal each bag.

[0230] For the control group, two identical bags of breadsticks were opened and resealed without introducing the adhesive composition.

[0231] Store the bagged breadsticks (test and control breadsticks) under the same conditions (ambient temperature / room temperature). Perform daily visual inspections to check for signs of mold formation and growth.

[0232] The control group showed the first signs of mold after 13 days of storage, and the mold was more pronounced after 20 days, almost completely covering the bread. Figures 3A to 3C It shows the time at zero point (after resealing), Figure 3A ), and after 13 days (the mold that has formed is circled), Figure 3B ) and after 20 days ( Figure 3C The differences between ) Figures 3A to 3C In each group, the two breadsticks on the left represent fresh / treated bread, and the two breadsticks on the right represent untreated (control) breadsticks. Compared to the significant mold formation in the untreated (control) breadsticks, the treated groups (including adhesive composition 3) showed no significant presence of mold. Figure 3C ).

[0233] sponge cake

[0234] The effect of adhesive composition No. 3 (fixed to the paper backing material) on the preservation of sponge cakes was also tested.

[0235] First, prepare the cake according to the manufacturer's instructions (Pillsbury) and let it cool to room temperature, then pack it in a polyethylene zip-lock bag.

[0236] Adhesive composition #3 was applied to the inside of two sponge cake bags (facing the top of the cake), and the bags were resealed. As a control group, a similar opening and resealing process was performed with two other sponge cake bags.

[0237] Then store the four bagged sponge cakes at room temperature for 15 days.

[0238] After 10 days, mold appeared on the cakes in the control group, while the cakes packaged with adhesive composition No. 3 did not show a similar amount of mold until 15 days later. That is, adhesive composition No. 3 increased the shelf life of the cakes by about 50%.

[0239] Figures 4A to 4C It shows the time at zero point (after resealing), Figure 4A ), after 10 days (the formation of mold is circled), Figure 4B ) and 15 days later (the mold was circled, Figure 4C (sponge cake). In Figure 4A and Figure 4C In each pair, the two cakes on the left represent preserved / treated cakes, and the two cakes on the right represent untreated (control) cakes. Figure 4B In the image, the cake on the left is a preservative-preserved cake, while the cake on the right is a comparison.

[0240] Compared to the untreated cake, no mold was found in the treated group after 10 days, which proves the preservation effect of adhesive composition No. 3.

[0241] Mushroom-shaped packaging

[0242] The effect of adhesive composition No. 3 on the preservation of mushrooms packaged in shrink film was also examined.

[0243] Holes were created in the shrink film of two mushroom-shaped packages, and adhesive composition No. 3 was attached to the outside of the shrink film by resealing the mushroom-shaped packages. As a control, mushrooms packaged with two shrink films showed no change.

[0244] Monitoring color changes (discoloration) on packaging is an immediate indicator of microbial activity (bacterial and / or mold growth).

[0245] Three days later, only the untreated / control packaging showed noticeable discoloration (not shown).

[0246] Example 2 – Fruit and Vegetable Preservation

[0247] Making food preservation stickers

[0248] Another sticker based on the currently disclosed theme has been prepared to include the following layers:

[0249] The first backing material comprises a polypropylene layer having a fixing element and a removable fixing liner. The first PP backing material is applied using an adhesive composition as detailed in Table 4, followed by the application of an additional kraft paper layer using an industrial laminator. The resulting stickers are then cut into circular stickers with a diameter of 35 mm.

[0250] Table 4: Adhesive Composition No. 4

[0251]

[0252]

[0253] Use this sticker in the fruit and vegetable storage experiment described below.

[0254] Specifically, the fruits or vegetables wrapped with stickers were called the "experimental" group, while the fruits or vegetables not wrapped with stickers were called the "control" group.

[0255] Extended shelf life of strawberries

[0256] The effects of stickers on pathogen colonization and damage in strawberries were evaluated.

[0257] Material :

[0258] Stickers – according to composition 4 in Table 4

[0259] Strawberries – Fresh strawberries without added fungicides or bactericides are transported under atmospheric conditions and used within 3 hours of harvest.

[0260] method

[0261] Strawberry Packaging: Freshly harvested strawberries weighing between 250g were packaged in designated clamshell containers (PET trays with plastic lids). In the experimental group, a sticker was positioned under the plastic lid.

[0262] The control group consisted of strawberries harvested from the same batch as the experimental group, but without labels. Both experimental and control packages were packaged in the same manner.

[0263] Storage conditions: Strawberries should be stored under different conditions (see also Table 6A):

[0264] -13℃, 75%RH (relative humidity);

[0265] -Ambient room temperature (22℃-25℃);

[0266] -Temperature fluctuations: Strawberries are stored at 4°C and 75%-90% RH and removed from the refrigerator at specified time intervals to 13°C to reflect occasional temperature changes that may occur in the supply chain and store displays.

[0267] The selected storage conditions were designed to promote bacterial growth caused by the stickers, while also replicating standard and extreme storage conditions along the supply chain.

[0268] Trial duration: The trial lasted from 3 to 13 days. The detailed duration of each trial is listed in Table 6A.

[0269] Experimental procedure: Six different experiments were conducted on a total of 10.5 kg of strawberries (250 g / pack), as detailed in Table 6A.

[0270] In both the control and experimental groups, strawberries were evaluated at different sampling intervals to:

[0271] Visual analysis This involves examining for color changes and signs of decay. Qualitative assessment is supplemented by photographic evidence.

[0272] Fruit microbial contamination testThe total microbial count, as well as the yeast and mold counts, were determined according to ISO standard 4833 (ISO 4833-1:2013, Microbiology of the food chain, Horizontal method for the enumeration of microorganisms).

[0273] Bacterial growth identification After fruit incubation, bacteria grown on the nutrient blend were extracted from the sticker and identified using any of the following: Gram staining protocol, catalase test, mannitol test, lechitanase test, and molecular identification using 16S sequencing (according to NCBI (National Center for Biotechnology Information) guidelines).

[0274] The experimental conditions are presented in Table 6A, and the results are presented in Table 6B.

[0275] Table 6A: Test Conditions

[0276]

[0277] *Each package contains 250 grams of product.

[0278] Table 6B: Results

[0279] Test Code Visual / bacterial test Strain identification (16S sequencing) Str.1 After 7 days, the mold count decreased to 1 / 2. Bacillus subtilis closely related species* Str.2 After 7 days, the mold rot decreased to 10 / 37. Bacillus subtilis closely related species* Str.3 After 3 days, the mold rot decreased to 10 / 27. Bacillus subtilis closely related species* Str.5A After 9 days, the mold rot decreased to 10 / 39. Bacillus subtilis closely related species* Str.5B After 12 days, the mold rot decreased to 5 / 17. Bacillus subtilis closely related species* Str.5C After 13 days, the mold rot decreased to 5 / 11. Bacillus subtilis closely related species*

[0280] *Identified through microscopic and biochemical tests.

[0281] Test groups Str.1, Str.2, and Str.3 were designed to test the performance of stickers on strawberries stored under different temperature conditions, and were conducted on the same batch of strawberries. In these tests, each package contained 250 grams of strawberries (per package).

[0282] In Str.1, according to ISO standard 4833, mold counts were performed on strawberries in the experimental and control groups at the starting point (T0), 3 days later (T3), and 7 days later (T7), representing extreme storage conditions. Results were... Figure 5 The average count is presented as 2 strawberry packages (at T0) or 3 strawberry packages (at T3, T7).

[0283] At T0, two packages of strawberries were sampled upon arrival at the laboratory.

[0284] At T3 and T7, three packages from the control group and three packages from the experimental group were removed from storage and sampled.

[0285] Figure 5The results showed that the stickers could inhibit mold growth on strawberries from the experimental group, with mold counts decreasing by 1.3 log and 1.6 log at T3 and T7, respectively. The inhibitory effect was significant starting from T3 and showed a marked extension at T7.

[0286] exist Figures 6A to 6D Mold growth inhibition is more easily observed in [the context of mold growth inhibition]. Figures 6A to 6D The results show the reduction in mold counts and mold colonies on the Str.1 test plate after 7 days compared to the control, including: total count in the control ( Figure 6A ), control yeast and mold ( Figure 6B ), total number of experiments ( Figure 6C ) and experimental yeasts and molds ( Figure 6D When mature mold colonies obtained from the control group ( Figure 6B ) and the inhibited Micrococcus colonies obtained from the experimental group ( Figure 6D When compared, the effect is shown.

[0287] These results indicate that the stickers inhibited the growth of spoilage microorganisms, such as mold, during long-term storage at 13°C. It is speculated that the mold inhibition in the experimental group was a result of microbial population transfer induced by the selective growth of Bacillus species naturally present around fruit on the nutrient blend. To verify Bacillus growth, microscopic, biochemical, and molecular tests were performed on the microbial populations growing inside the stickers in three parallel experiments.

[0288] Preliminary testing of the isolated colonies using Gram staining and microscopic imaging (data not shown) indicated the presence of Bacillus colonies growing from the nutrient blend. 16S sequencing confirmed the presence of a closely related species of Bacillus subtilis in Str.1.

[0289] Microbial growth in the nutrient blend was shown to have the following characteristics:

[0290] -Grow a single type of colony from all 3 triplet samples.

[0291] - At T3 and T7, the same type of single colonies were grown on nutrient blends from a total of 6 different treated strawberry packages.

[0292] - Colony morphology: Large, white and asymmetrical, with wavy edges and opaque texture.

[0293] -Microscopic appearance: Gram-positive staining, with the shape of a spore-forming bacillus.

[0294] - Biochemical tests: Catalase - positive, Lecitinase - negative, Mannitol - positive.

[0295] Biochemical and microscopic analyses showed that the Bacillus isolates of one type from all the test bags had the same characteristics.

[0296] -16S sequencing results confirmed the growth of Bacillus subtilis in the nutrient blend.

[0297] Tests Str.2 and Str.3 were designed to evaluate the effectiveness of the stickers in reducing visual signs of decay. The tests were conducted at different temperature conditions (13°C / 75%RH, 25°C / 75%RH) representing extreme temperature conditions.

[0298] In these experiments, the control and experimental groups each contained 6 packages of strawberries. Each group was divided into 2 subgroups, which were then stored at 13°C (each subgroup contained a total of 6 packages at each temperature).

[0299] Figures 7A to 7D and Figures 8A to 8D Strawberries stored at 13°C and 25°C (ambient temperature) are shown as part of tests Str.2 and Str.3. In these tests, each package contained 250 grams of strawberries.

[0300] Specifically, Figure 7A The control group at T0 of test Str.2 is shown after storage at 13°C and 75% RH; Figure 7B The control group of test Str.2 T7 is shown after storage at 13°C and 75% RH; Figure 7C The experimental group at T0 of test Str.2 is shown after storage at 13°C and 75%RH. Figure 7D The experimental group of T7 in test Str.2 is shown after storage at 13°C and 75% RH.

[0301] More specifically, Figure 7A and Figure 7B The study showed that strawberries stored at 13°C in the control group exhibited significant signs of microbial spoilage after 7 days of storage. Figure 7B Of the 38 strawberries tested, 12 were moldy (31.6% rotten). In the experimental group ( Figure 7C , Figure 7D In this study, the presence of the sticker reduced the spoilage percentage to only 8.5%, with 4 out of 47 strawberries becoming moldy after 7 days of storage (mold spoilage reduced to 10 / 37). Figure 7D ).

[0302] Figures 8A to 8D The same batch of strawberries stored at 25°C is shown, followed by T3. It can be seen that, similar to those stored at 13°C, the strawberries in the control group ( Figures 8A to 8BIt showed significant signs of microbial spoilage after 3 days of storage. Figure 8B Of the 43 strawberries tested, 21 were moldy (48.8% rotten). The sticker reduced the rotten percentage to only 17.7%, with 8 out of 45 strawberries becoming moldy after 3 days of storage (17.7% rotten, mold rot reduced to 10 / 27). Figure 8D ).

[0303] Temperature variations are unavoidable during the transportation of fresh produce and at store displays. Tests 5A, 5B, and 5C were designed to evaluate the effectiveness of stickers under such variable temperature conditions encountered during transportation.

[0304] In each test, the fruit was stored at 4°C for 5 days, and then at 13°C for 4 days (Test Str.5A), 7 days (Test Str.5B), and 8 days (Test Str.5C) at variable time intervals to evaluate the sticker performance during the shelf life.

[0305] In these experiments, both the control and experimental groups contained 12 strawberry packages. Each group was further divided into 3 subgroups.

[0306] Figures 9A to 9D The study showed that strawberries were stored at 4°C for 5 days, then at 13°C for 4 days, for a total of 9 days (T9), during which 16 out of 65 strawberries became moldy (24.8% rotten). In the control group ( Figure 9B In this study, the presence of the sticker reduced the spoilage percentage to only 6.3%, with 4 out of 63 moldy strawberries becoming moldy after a total of 9 days of storage (6.3% spoilage, mold spoilage reduced to 10 / 39). Figure 9D ).

[0307] Figures 10A to 10D The study showed that strawberries were stored at 4°C for 5 days, then at 13°C for 7 days, with a total follow-up of 12 days (T12). Of the 64 strawberries stored, 13 were moldy (20.3% spoilage). In the control group (… Figure 10B In the experimental group, the presence of the sticker reduced the spoilage percentage to only 5.9%, with 4 out of 68 strawberries becoming moldy after a total of 12 days of storage (mold spoilage reduced to 5 / 17), as shown in the experimental group. Figure 10D ).

[0308] Figures 10A to 10D The study showed strawberries stored at 4°C for 5 days, then at 13°C for 7 days, with a follow-up of 12 days (T12). Results showed that 21 out of 54 strawberries were moldy (38.9% rotten). In the control group ( Figure 10BIn this study, the presence of the sticker reduced the spoilage percentage to only 17.5%, with 10 out of 57 moldy strawberries becoming moldy after a total of 12 days of storage (mold spoilage reduced to 5 / 11). Figure 10D ).

[0309] Microscopic and biochemical tests on the bacteria produced on the nutrient blend confirmed the growth of Bacillus subtilis relatives in all storage intervals.

[0310] Extended shelf life of cherry tomatoes

[0311] The study also evaluated the effects of stickers on pathogen colonization and damage to cherry tomatoes. .

[0312] Material :

[0313] Stickers – according to composition 4 in Table 4

[0314] Cherry tomatoes – purchased from local supermarkets, packaged in sealed PET trays, specifically with perforated plastic film, each sealed package weighing 500 grams, and originating from the same harvest batch.

[0315] method

[0316] Cherry tomato packaging: Cherry tomatoes were divided into control and experimental groups. Stickers were affixed to the inside of a sealing plastic film by making small cuts in the film. The control group consisted of cherry tomatoes harvested from the same batch as the experimental group, but without stickers. Both experimental and control packages were packaged in the same manner.

[0317] Storage conditions: For all groups, cherry tomatoes should be stored at 13°C and 75% RH (relative humidity).

[0318] Trial duration: Trials lasted from 6 to 10 days. The detailed duration of each trial is listed in Table 7A.

[0319] Experimental Procedure: A total of 26 kg (52 units) of cherry tomatoes (500 g / pack) were subjected to four different experiments, as detailed in Table 7A. Cherry tomatoes were evaluated at different sampling intervals in both the control and experimental groups to:

[0320] Visual analysis: This involves examining color changes and signs of decay. Qualitative assessment is supplemented by photographic evidence.

[0321] Fruit microbial contamination test: Total microbial count, as well as yeast and mold count, were determined according to ISO standard 4833.

[0322] Bacterial growth identification: After fruit incubation, bacteria grown on the nutrient blend were extracted from the sticker and identified using the following methods: Gram staining protocol, catalase test, mannitol test, lechitanase test, and molecular identification using 16S sequencing (according to NCBI (National Center for Biotechnology Information) guidelines).

[0323] Table 7A: Test Conditions

[0324]

[0325] *Each package contains 500 grams of product.

[0326] Table 7B: Test Results

[0327] Test Code Visual / bacterial test Strain identification (16S sequencing) Tmt.1 The visible mold on the board was reduced to 2 / 3. Bacillus subtilis closely related species Tmt.2 The spoilage rate decreased to 10 / 29 Bacillus subtilis closely related species Tmt.3 Visible mold on the board decreased to 25 / 74 Bacillus subtilis closely related species Tmt.4 The spoilage rate decreased to 10 / 19 Bacillus subtilis closely related species

[0328] *Identified through microscopic and biochemical tests.

[0329] According to ISO standard 4833, mold counts were performed on the experimental and control groups at the start point (T0), after 3 days (T3), and after 6 days (T6). Results were... Figure 12 The average count is presented as 2 cherry tomato packages (at T0) or 3 cherry tomato packages (at T3, T6).

[0330] At T0, two packages of tomatoes were sampled upon arrival at the laboratory.

[0331] At T3 and T6, three packages from the control group and three packages from the experimental group were removed from storage and sampled.

[0332] Figure 12 The results showed that the stickers were able to inhibit mold growth on cherry tomatoes from the experimental group, with the mold count decreasing by 2.7 log at T3 and by 1.5 log at T6.

[0333] Based on the test plates identified as Tmt.1, when comparing the number of mold colonies obtained from the control group ( Figure 13B ) and the number of limited mold colonies obtained from the experimental group ( Figure 13D At that time, Figures 13A to 13D Mold growth inhibition is more easily observed in [the context].

[0334] These results indicate that the stickers inhibited the growth of spoilage microorganisms, such as mold, during long-term storage at 13°C. It is speculated that the mold inhibition in the experimental group was a result of microbial population transfer induced by the selective growth of Bacillus species naturally present around the fruit on the nutrient blend. To verify Bacillus growth, microscopic, biochemical, and molecular tests were performed on the microbial population growing inside the stickers.

[0335] Gram staining and microscopic imaging were used to test the isolated colonies, indicating the presence of Bacillus colonies growing from the nutrient blend.

[0336] Microbial growth in the nutrient blend was shown to have the following characteristics:

[0337] -Grow a single type of colony from all 3 triplet samples.

[0338] - At T3 and T6, the same type of single colonies grew on nutrient blends from a total of 6 different treated cherry tomato packages. Colony morphology: large, white, and asymmetrical.

[0339] It features wavy edges and an opaque texture.

[0340] -Microscopic appearance: Gram-positive staining, with the shape of a spore-forming bacillus.

[0341] - Biochemical tests: Catalase - positive, Lecitinase - negative, Mannitol - positive

[0342] Biochemical and microscopic analyses showed that the Bacillus isolates of one type from all the test bags had the same characteristics.

[0343] -16S sequencing results confirmed the growth of Bacillus subtilis relatives in the nutrient blend in experiment Tmt.1.

[0344] The purpose of Test Tmt.2 is to evaluate the quality of the same cherry tomatoes within their shelf life based on market acceptability for each sales unit.

[0345] Cherry tomatoes were stored at 13°C / 75% relative humidity for 10 days and then classified into subgroups based on their corresponding quality scores compared to the control and experimental groups.

[0346] Images (not shown) were taken to document the visual appearance. Each cherry tomato in each tray was rated on a scale of 1-4, where:

[0347] 1- Signs of severe decay

[0348] 2- Moderate signs of decay

[0349] 3. Slight signs of decay, soft to the touch

[0350] 4. Firm berries with no signs of rot

[0351] The results are also summarized in Table 8, which indicates the number of cherry tomatoes in each box for each rating. Further indications include the total number of tomatoes in each box for each rating (“Total”), the percentage of spoiled tomatoes considering tomatoes at ratings 1 and 2 (“Spoiled%”); the average number of spoiled cherry tomatoes (below ratings 1 and 2) per box (“Average Spoiled”), the percentage of high-quality tomatoes (i.e., rating 4) per box (“High-Quality%”), and the average number of high-quality tomatoes per box (“Average High-Quality”). Table 8: Results of the 10-Day Follow-up of Experiment Tmt.2

[0352]

[0353]

[0354] Table 8 shows that the stickers disclosed in this paper reduced the percentage of corrosion from 22.2% in the control group to 7.6% in the experimental group, a reduction of 2.9 times.

[0355] Furthermore, the currently disclosed stickers enhanced the overall superiority of cherry tomatoes, which was evident in the comparison of fruits rated 4 (the highest score) between the control and experimental groups. This percentage increased from 43.1% in the control group to 59.3% in the experimental group, representing a 1.4-fold improvement (see Table 8 above).

[0356] The purpose of experiments Tmt.3 and Tmt.4 was to demonstrate the reproducibility of the results using the same testing method. The cherry tomatoes used in these experiments came from the same batch of harvest.

[0357] Figure 14 The bar graph shows that the stickers were able to inhibit mold growth on cherry tomatoes from the experimental group, with the mold count decreasing by 1.9 log at T3 and by 2.9 log at T6.

[0358] Based on the test plates identified as Tmt.3, when comparing the number of mold colonies obtained from the control group ( Figure 15B ) and the number of limited mold colonies obtained from the experimental group ( Figure 15D At that time, Figures 15A to 15D Mold growth inhibition is more easily observed in [the context].

[0359] These results indicate that the sticker repeatedly inhibits the growth of putrefactive microorganisms (such as mold) during long-term storage at 13°C.

[0360] Preliminary tests on the isolated colonies using Gram staining and microscopic imaging indicated the presence of Bacillus colonies growing from the nutrient blend.

[0361] The results of Tmt.4 are summarized in Table 9.

[0362] Table 9 – Results of the 9-day follow-up of Experiment Tmt.4 :

[0363]

[0364]

[0365] Table 9 shows that the sticker reduced the percentage of decay from 29.3% to 15.5%, a reduction of 1.89 times.

[0366] Furthermore, the stickers enhanced the overall quality of the cherry tomatoes, which was evident in the comparison of fruits rated 4 (the highest score) between the control and experimental groups. This percentage increased from 34.9% in the control group to 51.1% in the experimental group, representing a 1.5-fold improvement.

[0367] Extended shelf life of mangoes

[0368] The study also evaluated the effects of the stickers on the colonization and damage of non-pathogenic bacteria on mangoes.

[0369] Material :

[0370] Sticker: According to composition 4 in Table 4

[0371] Mangoes – Four units of fresh mangoes purchased from the same batch of harvest from the local market.

[0372] method :

[0373] Experimental group: Stickers were directly attached to the skins of two mangoes, and then they were placed in a polyethylene bag.

[0374] Control group: The remaining mangoes from the same batch were packaged similarly without stickers and used as a control group.

[0375] Storage conditions: Throughout the study, the storage conditions were 25°C / 75%RH.

[0376] Experimental procedure: The color and signs of decay of mangoes were visually assessed at 5 days (T5), 7 days (T7), and 10 days (T10). Control group images were taken at different time points. Figure 16A ) and experimental group ( Figure 16B ) photos for recording ( Figures 16A to 16B ).

[0377] Figures 16A to 16B This indicates that the control group ( Figure 16A Mangoes begin to show signs of discoloration and rotting at T5. This condition worsens at T7 and T10.

Claims

1. A sticker comprising at least one adhesive component, the at least one adhesive component comprising a carrier composition containing a nutrient composition; wherein the carrier composition comprises a food-grade adhesive, and the nutrient composition comprises at least one compound suitable for supporting the selective growth of one or more human-safe bacteria.

2. The sticker according to claim 1, wherein the carrier composition comprises a food-grade desiccant.

3. The sticker according to claim 1 or 2, wherein the food-grade adhesive is selected from the group consisting of polysaccharides, proteins, waxes, and any combination thereof.

4. The sticker according to claim 3, wherein the food-grade adhesive comprises at least one polysaccharide.

5. The sticker according to claim 4, wherein the at least one polysaccharide is selected from the group consisting of: starch, alginate, pectin, carrageenan, locust bean gum, guar gum, xanthan gum, dextrin, agarose, agar, amylopectin, cellulose, sodium carboxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, alginate, 1,2-propanediol alginate, tragacanth gum, chitosan, gum arabic, arabic gum, tara gum, gellan gum, and any combination thereof.

6. The sticker of claim 3, wherein the food-grade adhesive comprises at least one protein.

7. The sticker of claim 6, wherein the at least one protein is selected from the group consisting of: animal-derived proteins, plant-derived proteins, insect-derived proteins, algal-derived proteins, and any combination thereof.

8. The sticker according to claim 6 or 7, wherein the at least one protein is selected from the group consisting of: rice, corn, wheat, barley, oats, sorghum, buckwheat, amaranth, quinoa, peas, chickpeas, lentils, lupins, flaxseed, soybeans, peanuts, sunflower seeds, cotton, sesame seeds, chia seeds, pumpkin seeds, almonds, chestnuts, pecans, walnuts, gelatin, casein, whey protein, beef protein, bovine protein, and any combination thereof.

9. The sticker of claim 3, wherein the food-grade adhesive comprises at least one wax.

10. The sticker of claim 9, wherein the at least one wax is selected from the group consisting of: beeswax, carnauba wax, candelilla wax, soy wax, shellac, microcrystalline wax, and any combination thereof.

11. The sticker according to claim 2 or any one of claims 3 to 10 when dependent on claim 2, wherein the hygroscopic agent is selected from the group consisting of at least one polyol, at least one polysaccharide, at least one salt, and any combination thereof.

12. The sticker of claim 11, wherein the polyol is selected from the group consisting of glycerol, sorbitol, propylene glycol, lactitol, xylitol, erythritol, isomaltitol, mannitol, maltitol, and any combination thereof.

13. The sticker according to claim 11 or 12, wherein the polysaccharide is selected from the group consisting of maltodextrin, dextrin, polydextrose, and any combination thereof.

14. The sticker of claim 13, wherein the at least one food-grade adhesive comprises dextrin.

15. The sticker of claim 14, wherein the amount of dextrin is about 50% to 70% by weight of the total weight of the adhesive component, preferably about 55% to 65% by weight of the total weight of the adhesive component.

16. The sticker according to claim 11, wherein the at least one salt is selected from the group consisting of: potassium gluconate, calcium gluconate, sodium gluconate, potassium acetate, sodium acetate, calcium acetate, sodium lactate, sodium citrate, potassium citrate, calcium citrate, sodium maleate, potassium maleate, calcium maleate, potassium aluminum silicate, aluminum silicate, calcium glutamate, monoammonium glutamate, magnesium diglutamate, sodium acetate, sodium chloride, calcium chloride, and any combination thereof.

17. The sticker according to any one of claims 1 to 16, wherein the food-grade adhesive comprises at least one salt.

18. The sticker according to claim 17, wherein the at least one salt is sodium chloride.

19. The sticker of claim 18, wherein the amount of sodium chloride is about 1% to 15% by weight of the total weight of the adhesive assembly, preferably about 2% to 10% by weight of the total weight of the adhesive assembly.

20. The sticker according to any one of claims 1 to 19, wherein the nutritional composition is blended within the carrier composition.

21. The sticker according to any one of claims 1 to 20, wherein the nutritional composition comprises a combination of compounds selected from the group consisting of nitrogenous compounds, carbohydrates, inorganic minerals, inorganic salts, fatty acids, and vitamins.

22. The sticker of claim 21, wherein the nutritional composition comprises at least one nitrogen-containing compound.

23. The sticker according to claim 21 or 22, wherein the at least one nitrogen-containing compound is selected from the group consisting of at least one amino acid, at least one peptide, at least one polypeptide, and at least one protein hydrolysate.

24. The sticker according to any one of claims 21 to 23, wherein the nutritional composition comprises at least one carbohydrate.

25. The sticker of claim 24, wherein the at least one carbohydrate is selected from the group consisting of monosaccharides, disaccharides, and oligosaccharides.

26. The sticker of claim 25, wherein the at least one carbohydrate is selected from the group consisting of: mannitol, arabinose, xylose, glucose, galactose, maltose, raffinose, sucrose, lactose, fructooligosaccharides (FOS), and sorbitol.

27. The sticker according to any one of claims 21 to 26, wherein the nutritional composition comprises at least one inorganic mineral and / or at least one inorganic salt.

28. The sticker of claim 27, wherein the at least one inorganic mineral and / or at least one inorganic salt comprises any or a combination of phosphates, sulfates, potassium, calcium, zinc, magnesium, manganese, and iron.

29. The sticker according to any one of claims 21 to 28, wherein the nutritional composition comprises at least one fatty acid.

30. The sticker according to claim 29, wherein the at least one fatty acid is selected from the group consisting of: caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, arachidic acid, behenic acid, oleic acid and linoleic acid.

31. The sticker according to any one of claims 21 to 30, wherein the nutritional composition comprises at least one vitamin.

32. The sticker according to claim 31, wherein the at least one vitamin is selected from the group consisting of: nicotinic acid (vitamin B13), calcium pantothenate (calcium salt of vitamin B5), pyridoxine (vitamin B6), and vitamin B12.

33. The sticker according to any one of claims 1 to 32, wherein the nutritional composition comprises at least one surfactant.

34. The sticker according to claim 33, wherein the at least one surfactant is selected from the group consisting of: polyoxyethylene (20) sorbitol monolaurate, polyoxyethylene (40) sorbitol monopalmitate, polyoxyethylene (60) sorbitol monostearate, polyoxyethylene (20) sorbitol monooleate and lecithin.

35. The sticker according to any one of claims 1 to 34, wherein the nutritional composition comprises a buffer.

36. The sticker of claim 35, wherein the buffer comprises any one or a combination of phosphoric acid, citric acid, lactic acid, and glycine.

37. The sticker according to any one of claims 1 to 36, wherein the nutritional composition has a pH in the range of about 5.5 to about 7.

38. The sticker according to any one of claims 1 to 37, wherein the adhesive assembly has an adhesive assembly first side and an opposing adhesive assembly second side.

39. The sticker of claim 38, further comprising a first backing material disposed on a first side of the adhesive assembly, the first backing material having a first backing material first side facing the adhesive assembly and an opposing first backing material second side.

40. The sticker of claim 39, wherein the first backing material comprises materials selected from the group consisting of: paper, fabric, synthetic polymers, and silicone.

41. The sticker of claim 39 or 40, further comprising a first fastening element disposed on a second side of the first backing material, the first fastening element having a first fastening element first side facing the first backing material and an opposing first fastening element second side configured to fasten the sticker to a consumer product.

42. The sticker of claim 41, further comprising a removable first fastening element liner disposed on a second side of the first fastening element, the removable first fastening element liner being configured to be removed before the sticker is applied to a consumer product.

43. The sticker of any one of claims 38 to 42, further comprising a removable adhesive assembly liner disposed on a second side of the adhesive assembly, the removable adhesive assembly liner being configured to be removed before the sticker is applied to a consumer product.

44. The sticker according to any one of claims 39 to 42, the sticker further comprising a second backing material disposed on a second side of the adhesive assembly, the second backing material having a first backing material side facing the adhesive assembly and an opposite second backing material side.

45. The sticker of claim 44, the sticker comprising a second fastening element disposed on a second side of the second backing material, the second fastening element having a second fastening element first side facing the second backing material and an opposing second fastening element second side configured to fasten the sticker to a consumer product.

46. ​​The sticker of claim 45, further comprising a removable second fastening element liner disposed on a second side of the second fastening element, the removable second fastening element liner being configured to be removed before the sticker is applied to a consumer product.

47. A consumer product package comprising a packaging element for containing a consumer product to be preserved and a sticker adhered to the packaging element according to any one of claims 1 to 46.

48. The consumer product packaging of claim 47, wherein the sticker is adhered to the packaging element on the inside of the packaging.

49. The consumer product packaging of claim 47, wherein the sticker is adhered to the outer side of the packaging to the packaging element, and a moisture channel is provided between the inner volume of the packaging and the adhesive assembly for activating the nutritional composition by water vapor.

50. The packaging of a consumer product according to any one of claims 47 to 49, wherein the consumer product includes food.

51. The consumer product packaging of claim 50, wherein the food is selected from the group consisting of: vegetables, fruits, herbs, grains, baked goods, meat and poultry, fish, dairy products, sauces and condiments.

52. The consumer product packaging according to any one of claims 47 to 51, wherein the shelf life of the consumer product is extended by at least 10% compared to the same consumer product packaged and stored under the same conditions, in which the carrier composition does not contain the nutritional composition.

53. A consumer product comprising food to be preserved, wherein a sticker according to any one of claims 1 to 46 is adhered to the surface of the food.

54. The consumer product of claim 53, wherein the food is selected from the group consisting of: vegetables, fruits, baked goods, meat and poultry, and fish.

55. A method for preserving a consumer product, the method comprising adhering a sticker according to any one of claims 1 to 46 to a package containing the consumer product, the adhesion being performed in a manner that allows moisture channels to be formed between the consumer product and the adhesive assembly.

56. A method for manufacturing a food safety sticker, the method comprising mixing a carrier composition comprising a food-grade adhesive with a nutrient composition comprising at least one compound suitable for supporting the selective growth of one or more human-safe bacteria to form an adhesive assembly, and shaping the adhesive assembly.

57. The method of claim 56, the method comprising depositing a removable lining and / or backing material onto at least one side of the adhesive composition.

58. A method for manufacturing food safety stickers, the method comprising: An adhesive assembly is provided, the adhesive assembly comprising a carrier composition that contains a nutrient composition; The carrier composition comprises a food-grade binder, and the nutrient composition comprises at least one compound suitable for supporting the selective growth of one or more human-safe bacteria; and At least one of a first backing material and a first removable liner is disposed on at least one side of the adhesive assembly, and optionally at least one of a second backing material and a second removable liner is disposed on a second opposite side of the adhesive assembly. The first backing material and the second backing material may be the same or different, and the first removable liner and the second removable liner may be the same or different.

59. The method of claim 58, the method comprising disposing at least one fastening element on at least one of the first backing material and the second backing material, the fastening element optionally having a removable lining on a side opposite to the at least one of the first backing material and the second backing material.

60. The method of claim 59, the method comprising disposing a first backing material on a first side of the adhesive assembly and disposing a removable liner on an opposite second side of the adhesive assembly.

61. The method of claim 60, the method comprising attaching a fastening element to a second side of the first backing material opposite to the side having the adhesive assembly, the fastening element carrying a removable lining on the side opposite to the backing material.

62. The method according to any one of claims 58 to 61, wherein the adhesive component is defined as any one of claims 1 to 38.

Images